Palm MicroBeam User Manual
PALM
®
MicroBeam
User Manual
PALM® MicroBeam
User Manual - 0202 Z
2 PALM® MicroBeam User Manual - 0202 Z
This document is delivered only to persons who are trained and authorized by P.A.L.M. Microlaser Technologies AG.
No part of this document may be reproduced or distributed in any form or by any means without the prior
written consent of P.A.L.M. Microlaser Technologies AG.
The information in this document is subject to change without notice. P.A.L.M. Microlaser Technologies AG
assumes no responsibility for any errors that may appear.
Printed June 2002
Second Edition
The following technologies have been patented by P.A.L.M. Microlaser Technologies:
- Laser catapult technology (Laser Pressure Catapulting LPCpat)
Patents: US 5,998,129, EP 879408 B1 and others.
- Three-dimensional laser beam positioning system
Patents: US 5,689,109, EP 679325 B1 and others.
- Additional patents pending.
P.A.L.M. Microlaser Technologies AG
Am Neuland 9+12
82347 Bernried / Germany
Phone: +49-(0)8158-9971-0
Fax: +49-(0)8158-9971-249
E-mail: info@palm-microlaser.com
www.palm-microlaser.com
©P.A.L.M. Microlaser Technologies AG, 2001. All rights reserved.
PALM® MicroBeam User Manual - 0202 Z 3
List of Contents
Content
Chapter Page
1 Important Basic Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Legal Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Conventions for this User Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 How You Can Contact Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3 Operating Room . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3.1 Admissible Ambient Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
4 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
4.1 What You Should Do in an Emergency Case . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
4.2 Observe the User Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
4.3 Laser Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
4.4 Safety Markings and Type Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.5 Qualification of the Staff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.6 Points that must strictly be observed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.7 Pay Attention to Remaining Risks and Preventive Measures . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.7.1 Remaining Risks and Protective Measures – Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.7.2 Remaining Risks – Electricity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.7.3 Remaining Risks – Infectious Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4.7.4 Remaining Risks – EMV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.7.5 Remaining Risks – Fire Extinction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.8 How to Ensure a Safe and Ecological Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5The PALM
®
MicroBeam - Survey. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
5.1 PALM
®
ControlUnit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.2 Laser Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5.3 Laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
5.4 Microscope Adapter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
5.5 Microscope Zeiss Axiovert 200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5.5.1 Fluorescence attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.6 PALM
®
RoboStage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.7 PALM
®
CapMover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.7.1 Control Apparatus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.8 Mechanical Manipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.9 CCD Video Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.10 Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6 How to Prepare the PALM
®
MicroBeam for the Application . . . . . . . . . . . . . . . . . . . . . . . 18
6.1 Switch On/Off the Device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.1.1 Switch On the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.1.2 Switch Off the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.2 CCD Video Camera Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.3 Check of the Laser in the Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.4 Laser energy and focus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.4.1 Laser Energy Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.4.2 Laser Focus Correction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.5 Adjust Laser Focus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.6 Cutting speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4 PALM® MicroBeam User Manual - 0202 Z
List of Contents
7PALM® MicroBeam with Fluorescence Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.1 Operate the Microscope with Fluorescence Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.1.1 Simultaneous Fluorescence Observation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
8 Trouble Shooting List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
8.1 Laser is not cutting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
8.2 No video image . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.3 Shift between drawing and cutting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
9 Maintenance of the PALM
®
MicroBeam . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.1 Laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.2 Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.2.1 Cleaning of the Optical Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
9.2.2 Cleaning When Actually Dirty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
10 Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
10.1 Electricity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
10.2 Nitrogen Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
PALM® MicroBeam User Manual - 0202 Z 5
Important Basic Information Legal Notes
1 Important Basic Information
Congratulations! With your new PALM® MicroBeam you obtained the State-of-the-Art Technology in microdissection and micromanipulation. The
PALM
®
MicroBeam is a high precision instrument,
which allows to perform microdissection in nearly
every kind of research as well as in routine examination with a high throughput.
To meet a very high safety standard and to use all
the benefits of your PALM
®
MicroBeam you should
read this user´s manual carefully and observe all
instructions given in this manual. Please follow
also the instructions for the single components of
your PALM
®
MicroBeam especially for the microscope, the fluorescence attachment (option), the
video camera and the laser. P.A.L.M. Microlaser
Technologies will not be responsible for mistakes
and possible consequences caused by non-observance of the user´s manual.
Please consider also the manuals provided for your
periphery devices!
1.1 Legal Notes
P.A.L.M. Microlaser Technologies AG is not liable
for damages resulting from the fact that this user
manual was not or only partly observed.
The warranty extinguishes in case the customer
opens the laser housing or uses the device for
other than the specified purposes.
If used parts are replaced by similar parts which
are, however, not identical to those recommended
by the manufacturer in this manual, P.A.L.M.
Microlaser Technologies AG is only liable if the
product supplied has already been faulty before
the parts were replaced.
For details concerning the manufacturers warranty, please refer to the contractual conditions.
1.2 Conventions for this User Manual
Special markings on the left margin point out
information you should observe by all means:
1.3 How You Can Contact Us
Warning
This sign warns you of possible most serious,
irreversible injuries to persons even going as
far as to death.
Caution
This sign signalizes possible moderate personal
damage and possible damage of the device.
Notice
This sign signalizes that information is given
demanding your special attention.
Info:
This indicates tips on correct use and offers
useful notes for getting the best use of the software.
Business hours 9 h - 15.00 h, Mo - Fr
Phone +49 (0) 81 58 99 71-100
Fax +49 (0) 81 58 99 71-249
E-mail Info@palm-microlaser.com
support@palm-microlaser.com
Service Address P.A.L.M. Microlaser Technolo-
gies AG
Am Neuland 9 + 12
82347 Bernried
Germany
Homepage www.palm-microlaser.com
6 PALM® MicroBeam User Manual - 0202 Z
Installation
2Installation
Your PALM® MicroBeam is only to be installed by
Palm service engineers or their representatives. In
case you want to move an already installed unit
please contact P.A.L.M. Microlaser Technologies
for assistance.
The PALM
®
Laser Systems basically are maintenance-free. Necessary service actions are the
exchange of the laser cartridge which has a garanteed lifetime of two years or 20·10
6
pulses.
In case of any malfunction of the device, please contact the PALM
®
Service (see section 1.3, page 5).
3 Operating Room
In order to guarantee a correct function and comfortable work with your new PALM
®
MicroBeam
System, the following requirements has to be paid
attention:
- The system should be installed in a room, which
is adequately large (about 6 square meters)
and well aired.
- There should be a free space available about
90cm above the table and all shelves, wall cupboards etc. should be removed
Figure 1 Dimensions
- Nature of the floor: vibration free (if not, spe-
cial working table recommended) and even
- Load carrying capacity for steel or granite
table: < 400 kg
- Minimum space needed in total: for steel or
granite table about 140 x 90 cm or for the aluminum plate a table measuring at least 120 x
90 cm on which the system will be set up and
with a load carrying capacity of 90 kg.
- Power supply: 115/230VAC / 16VA safety fuse
3.1 Admissible Ambient Conditions
The divice may be operated at the following ambient conditions:
- Ambient Temperature of the air:
+15°C to +25°C
- Air humidity: 30% to 80%, not condensing.
Notice
Please consider also vibrations raising from
your routine lab equipmemt!
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PALM® MicroBeam User Manual - 0202 Z 7
Safety What You Should Do in an Emergency Case
4Safety
4.1 What You Should Do in an
Emergency Case
• Switch off the device immediately and make
sure that it cannot be switched on again.
• Unplug the multiple socket.
• Call the service staff in charge.
4.2 Observe the User Manual
This user manual contains important information
concerning the safe operation of the device. Only
when strictly observing the user manual, accidents
can be avoided and a trouble-free operation of the
device can be guaranteed. By observing the user
manual, downtimes and repair costs will be
reduced and the service life of the device
increased.
This user manual must be stored close to the
device at a place accessible and known to the staff,
where it is available at any time.
This user manual must be read, understood and
observed in all points by the responsible and operating staff.
Please observe all safety instructions and other
information given in the user manual and the
attached manuals of the accessories.
4.3 Laser Safety
The PALM® MicroBeam is a laser device of Class 1 M
with no special safety requirements if operated
according to the instructions given in this user manual.
Built in is a Laser of class 3B. It bears a potential
risk. A direct or reflected beam must not strike the
unprotected eye (do not look directly into the
beam).
In service case, the device has to be operated by
the service staff or an authorized representative of
P.A.L.M. Microlaser Technologies AG even with the
laser casing open.
The customer must take the following safety measures in order to prevent any direct looking into
the beam or any uncontrolled mirrored reflections:
- The laser may only be operated in a surveyed
area.
- In case there are other work places located in
the operating room, the device must be
shielded by laser protective walls during service
works.
Only staff instructed in laser safety is allowed to
stay in the hazardous area when the laser casing is open.
Caution
Any use other than the ones defined in this user
manual might lead to personal injuries or
material damage which P.A.L.M. Microlaser
Technologies AG is not liable for.
Warning
The housing of the laser interface may only be
removed by the service staff or an authorized
representative of P.A.L.M. Microlaser Technologies AG.
8 PALM® MicroBeam User Manual - 0202 Z
Safety Safety Markings and Type Plate
4.4 Safety Markings and Type Plate
Make sure that the following safety markings and
the type plate exist and are legible. Replace missing or illegible safety markings or type plate.
Figure 2 Safety Markings and Type Plate
Caution
The safety markings on the device must always
be clearly visible and legible and must strictly
be observed.
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Class M1
Identification label
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