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Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Olympus AU 400 - 600 - 640 instructions
rev 422.0.2 - 2010-08-01
Warning!
These programs should be used only as guideline.
To check the reagent correct setting on the
instrument, use only good quality control sera
and work accordingly to with good laboratory
practice. Instrumental factor must be checked.
Chema Diagnostica will not be responsible for
instrumental bad programming.
IUS.422.0.2 Directions for use Edition 2010-08-01 page 3 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: Olympus AU 400 - 600 - 640
Application: ACID PHOSPHATASE - Codes AC 0120 TC
Preparation: POWDER SINGLE REAGENT. FOLLOW PREPARATION PROGRAM INDICATED IN INSERT
SHEET. INSTALL AS R1
Storage: REFRIGERATE AT 2-8°C
TEST NAME: ACP
Sample: Volume 20 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 200 ml Dilution 0 ml R2 volume 0 ml Dilution 0 ml
Wavelenght: Pri. 410 Sec. 800 Min OD Max OD
Method: RATE L - 0.1 H 2.5
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.3
Last L - 0.1 First H 0.3
Measuring point 1: First 12 Last 27
Measuring point 2: First Last
Linearity: 20% Dynamic range:
No Lag Time: NO L 1 H 60
Correlation factor:
A 1 B 0
On-board stability period: 30
Unit: U/L
Factor: 853
IUS.422.0.2 Directions for use Edition 2010-08-01 page 4 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: Olympus AU 400 - 600 - 640
Application: ALBUMIN - Codes BC 0100 / 0500 / 1000 / 1500 CH
Preparation: R1 - LIQUID READY TO USE SINGLE REAGENT
Storage: REFRIGERATE (2-8°C)
Stability: AS INDICATED IN THE LABEL
TEST NAME: ALB
Sample: Volume 2 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 295 ml Dilution 0 ml R2 volume 0 ml Dilution 0 ml
Wavelenght: Pri. 600 Sec. 800 Min OD Max OD
Method: END L H
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.5
Last L - 0.1 First H 0.5
Measuring point 1: First 0 Last 4
Measuring point 2: First Last
Linearity: Dynamic range:
No Lag Time: YES L 0.1 H 6.0
Correlation factor:
A 1 B 0
On-board stability period: 90
Unit: g/dl
IUS.422.0.2 Directions for use Edition 2010-08-01 page 5 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Preparation: R1 - INSTALL LIQUID READY TO USE REAGENT A
R2 - INSTALL LIQUID READY TO USE REAGENT B
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: ALP
Sample: Volume 5 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 200 ml Dilution 0 ml R2 volume 50 ml Dilution 0 ml
Wavelenght: Pri. 410 Sec. 480 Min OD Max OD
Method: RATE L -0.1 H 2.5
Reaction slope: + Reagent OD limit:
First L -0.1 First H 1.5
Last L -0.1 First H 1.5
Measuring point 1: First 13 Last 19
Measuring point 2: First Last
Linearity: 20% Dynamic range:
No Lag Time: NO L 5 H 3000
Correlation factor:
A 1 B 0
On-board stability period: 60
Unit: U/L
Factor: 2757
IUS.422.0.2 Directions for use Edition 2010-08-01 page 6 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Preparation: R1 - INSTALL LIQUID READY TO USE REAGENT A
R2 - INSTALL LIQUID READY TO USE REAGENT B
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: ALP
Sample: Volume 5 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 200 ml Dilution 0 ml R2 volume 50 ml Dilution 0 ml
Wavelenght: Pri. 410 Sec. 480 Min OD Max OD
Method: RATE L - 0.1 H 2.0
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 1.2
Last L - 0.1 First H 1.2
Measuring point 1: First 20 Last 24
Measuring point 2: First Last
Linearity: 20% Dynamic range:
No Lag Time: NO L 7 H 3000
Correlation factor:
A 1 B 0
On-board stability period: 30
Unit: U/L
Factor: 2757
IUS.422.0.2 Directions for use Edition 2010-08-01 page 7 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: Olympus AU 400 - 600 - 640
Application: AMYLASE FL - Codes AM F060 / F120 / F245 CHPreparation: R1 - LIQUID READY TO USE SINGLE REAGENT
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: AMY
Sample: Volume 6 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 240 ml Dilution 0 ml R2 volume 0 ml Dilution 0 ml
Wavelenght: Pri. 410 Sec. 480 Min OD Max OD
Method: RATE L - 0.1 H 2.0
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.3
Last L - 0.1 First H 0.3
Measuring point 1: First 5 Last 10
Measuring point 2: First Last
Linearity: 20% Dynamic range:
No Lag Time: NO L 10 H 3000
Correlation factor:
A 1 B 0
On-board stability period: 30
Unit: U/L
Factor: 3178
IUS.422.0.2 Directions for use Edition 2010-08-01 page 8 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Preparation: R1 - INSTALL LIQUID READY TO USE REAGENT A
R2 - INSTALL LIQUID READY TO USE REAGENT B
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: AMY
Sample: Volume 8 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 192 ml Dilution 0 ml R2 volume 48 ml Dilution 0 ml
Wavelenght: Pri. 410 Sec. 480 Min OD Max OD
Method: RATE L - 0.1 H 2.0
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.3
Last L - 0.1 First H 0.3
Measuring point 1: First 20 Last 27
Measuring point 2: First Last
Linearity: 20% Dynamic range:
No Lag Time: NO L 6 H 1500
Correlation factor:
A 1 B 0
On-board stability period: 30
Unit: U/L
Factor: 3480
IUS.422.0.2 Directions for use Edition 2010-08-01 page 9 of 41
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Preparation: R1 - INSTALL LIQUID READY TO USE REAGENT A
R2 - INSTALL LIQUID READY TO USE REAGENT B
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: AMYP
Sample: Volume 5 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 200 ml Dilution 0 ml R2 volume 50 ml Dilution 0 ml
Wavelenght: Pri. 410 Sec. 480 Min OD Max OD
Method: RATE L - 0.1 H 2.0
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.3
Last L - 0.1 First H 0.3
Measuring point 1: First 20 Last 27
Measuring point 2: First Last
Linearity: 20% Dynamic range:
No Lag Time: NO L 2 H 2500
Correlation factor:
A 1 B 0
On-board stability period: 30
Unit: U/L
Factor: 3480
IUS.422.0.2 Directions for use Edition 2010-08-01 page 10 of 4110 of 4141
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Preparation: R1 - INSTALL LIQUID READY TO USE REAGENT A
R2 - INSTALL LIQUID READY TO USE REAGENT B
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: BILT
Sample: Volume 10 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 200 ml Dilution 0 ml R2 volume 50 ml Dilution 0 ml
Wavelenght: Pri. 520 Sec. 800 Min OD Max OD
Method: END L H
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.5
Last L - 0.1 First H 0.5
Measuring point 1: First Last 27
Measuring point 2: First Last 10
Linearity: Dynamic range:
No Lag Time: L 0.05 H 20
Correlation factor:
A 1 B 0
On-board stability period: 60
Unit: mg/dl
IUS.422.0.2 Directions for use Edition 2010-08-01 page 12 of 4112 of 4141
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Preparation: R1 - INSTALL LIQUID READY TO USE REAGENT A
R2 - INSTALL LIQUID READY TO USE REAGENT B
Storage: REFRIGERATE AT 2-8°C
Stability: AS INDICATED IN THE LABEL
TEST NAME: BILD
Sample: Volume 10 ml Dilution 0 ml Pre-dilution Rate: 1
Reagents: R1 volume 200 ml Dilution 0 ml R2 volume 50 ml Dilution 0 ml
Wavelenght: Pri. 540 Sec. 660 Min OD Max OD
Method: END L H
Reaction slope: + Reagent OD limit:
First L - 0.1 First H 0.5
Last L - 0.1 First H 0.5
Measuring point 1: First Last 27
Measuring point 2: First Last 10
Linearity: Dynamic range:
No Lag Time: L 0.05 H 20
Correlation factor:
A 1 B 0
On-board stability period: 60
Unit: mg/dl
IUS.422.0.2 Directions for use Edition 2010-08-01 page 13 of 4113 of 4141
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