Olympus Ah-rfl-lb User Manual

VERTICAL

FLUORESCENCE

ILLUMINATOR

ATTACHMENT

This instruction manual has been written for use of the Vertical Fluorescence Illuminator Model

AH-RF

Therefore,
well

L-LB in conjunction with the Universal Research Microscope Model

it

is

recommended that you read the instruction manual for the VANOX AHB-LB as

as

this manual carefully in order

to

obtain optimum performance from this attachment, in

VANOX

AHB-LB.

conjunction with the microscope.

-

Note: This attachment is specially designed for use with

pieces,

LB

photo eyepieces, objectives, condensers,

etc.;

optics will result in unsatisfactory performance of the unit.

Observe the following points carefully:

8

Operation

1.

Use a D.C.

super pressure mercury burner, designated by OLYMPUS, i.e. HB0200Wl2

(manufactured by Osram) or USH200MB (manufactured by USHlO 1NC.lJapan).

2.

Ascertain that the burner

is

correctly inserted

tions are properly made.
Always handle the instrument with the care it deserves, and avoid abrupt motions.

3.

4.

Avoid exposure of the instrument to direct sunlight, high humidity and temperature, dust
and vibrations.

For protection of the observer's eyes from UV radiation, never look

5.

directly. Even when handling the specimen slides, be sure to look through the UV pro­tective shade, which blocks harmful UV radiation emitted from the mercury burner.

6.

Do not open the lamp housing while the mercury burner

is

minutes after the burner

switched off.

LB

series

use

of

optical components other than

and

clamped and that

optics,

is

lighted. Wait for about 10

including eye-

all

electric connec-

at

the exciting light

7.

Make sure that voltage and frequency selector switches are set to conform with the local

line voltage and frequency.

8.

Note that the Model AH-RFL-LB has a magnification factor of

magnification

is

the product of objective magnification times eyepiece magnification

1,2X,

thus the total visilal

times 1.2.

Maintenance

Be sure that no dirt, fingerprints, etc. are left on the lens and bulb surfaces.

1.

a

soiled, wipe them clean with
ether mixture

Never disassemble the attachment for repair by yourself. Only authorized OLYMPUS serv-

2.

(7:3)

or benzine.

cotton gauze. If necessary,

use

a

small amount of alcohol-

If

ice personnel should make repairs.

Replace the mercury burner after

3.

400

hours of use. Do not touch the burner for about 10

minutes after switching off.

4.

Disconnect the line cord from

5.

The instrument should be covered after use with the vinyl dust cover provided.

the

AC

power outlet for fuse replacement.

they are

CONTENTS

STANDARD

NOMENCLATURE

lDENTlFlCATtON

ASSEMBLY

OPERATION

B

.

Burner

C

.

Fluorescence

D

.

Burner

E

.

Use

EQUIPMENT

...............................................

...............................................

Centration

Replacement

of

Filters

.....................................

..........................................

AND

FUNCTION

OF

VARIOUS

COMPONENTS

........

.......................................

Microscopy

..................................

.....................................

..........................................

2

3

4

7

8

9

10

10

11

OPTICALDATA

SPECTRAL

CHARACTERISTICS

............................................

OF

FILTERS

......................

12

13

-

I.

STANDARD

EQUIPMENT

Vertical

fluorescence

revolving nosepiece)

Barr~er f ~lters

Non-f

luorescent

objectives

illuminator

(with

quintuple

AH-RFL-&-LB

11.

SPECIFICATIONS

1

.

Revolving

2.

Exciter

nosepiece:

filters

built

Quintuple

fication

in

turret:

on

of

objective

Code

U

V

B

G

ball

bearing;

all 5 positions

powers.

Exciter filter

UG

-

1

BG-3

BG

-12

1

F-545

+

6-36

letter

coded

for

easy

identi-

1

)

Dichroic

mirrors:

Mounted

in

slide

and

combined

with

--

built-in

barrier filters.

2)

Magnification

3.

Fluorescent

1)

D.C.

super pressure

mercury

2)

Power

111.

NOMENCLATURE

Model

Eyepiece

supply

AH-RFL-LB

7

Provided

factor:

light

source

burner:

unit:

on

with

a

1.2X

HB0200WJ2

factured

l

nput

(in

the

the

Universal

slot

for

by

voltage

U.S.A.,

Research

insertion

(manufactured

USHlO

AC

the

of

INC./Japan).

100V-3

power

Microscope

barrier

10V-120V

supply specifications

by

Osram)

Model

Standard

filters,

in

slide.

or

USH

or

220V-240V,

VANOX AHB-LB

observation

b

200MB

are

different.)

(manu-

50160

tube

Hz.

Fluorescence

Power

supply unit

vertical

Fluorescent

w

lamp

IDENTIFICATION

IV.

A.

Vertical

Fluorescence

AND

Illuminator

FUNCTION

OF

VARIOUS

COMPONENTS

@

Revolving

@

Observation tube clamping

@I

Barrier filter insertion slot:

@

UV

@

Dichroic

@

Field

@

Aperture iris diaphragm ring:

@

Exciter filter turret:

nosepiece:

protective

iris diaphragm ring:

shade:

mirror selector lever: Dichroic

rn

screw:

Provided

tives.
This ilfuminato~ is attached to the circular dovetail

of

the

Place

filter

Make

from fluorescent light.
tion

the dichroic mirror selector lever.

can

Incorporates filters

BG-3

IF-545

tional aperture

with

5

observation tube

the

dust

slide

is

used.

it a rule

of

a

be

selected

(V

+

to

barrrer

mirrors

with this lever.

for violet),

BC-36

(C

(0).

threaded apertures

and

clamped

l@

into this

use

this

shade

Can

fllter

in

mount

combined

UG-1

BG-12

for green violet), with

sfot

to protect your

be

swung

or

with4 built-in barrier filters

(*See

{coded

U

(6

for blue violet) and

to

accept

with

when

out for inser-

when

NOTE

for ultraviolet),

objec-

this

screw.

no

barrier

eyes

operatrng

below.)

an

addi-

@)

Filter mount:

Dust

slide

NOTE:

The

Model

length radiation
long wavelength.

AH-RFL-LB

towards

Accepts

incorporates dichroic mirrors,

the

two

objective

exciter filters

to

illuminate

the

in

mount.

which

reflect short

specimen, while

wave-

passing

B.

Fluorescent

Lamp

Hausing

"a

0

7

I

'.

-/

I-.

A'

Ij)

Knurled

a

Shutter

Collector lens
Burner centering

Socket

$I

Reflector centering

Reflector

@I

Connectors:

C.

Power

Supply

iing:

lever:

clamping screw

focusing knob

Attaches

Pull

focusing

knobs

knobs

Accept

Unit

out

knob

the

the

the

lamp

shutter

plugs

housing

lever

of

the

to

the

microscope

to

block

connecting

the

cord.

stand

light.

7:

.

,

Pilot lamp

('2)

Start

8%

Main

5$

Fuse

'3

Connecting

@

Frequency

button

switch

holder

cords

selector

switch

7~

Line

(@

Power

5)

Groundinq

%'

Grounding

Power

voltage

selector

cord connector

terminal

cord

cord

switch

D. Auxiliary

F.

Barrier

Collector

Filters

in

Lens

Slide

(10

pcs.)

E.

Exciter

G.

Gntering

Filters

Mirror

in

Mount

(6

pcs.)

V.

ASSEMBLY

Prior to

with your fingers.

1.

2.

3.

assembly,

Attach the lamp housing. (Fig.

Place the lamp housing next to the flange of the

opening provided on the microscope limb, with

positioning groove aligned with positioning pin,

and lock by turning the knurled ring clockwise.

Mount: the auxiliary collector lens. (Fig.

Insert the lens into the front of the opening on

the microscope limb.

Attach

1)

2)

3)

the observation tube.

Remove

the

microscope stand, and turn the selector

turret

on

tion

"M.P.".

Check

hand

(levers

Insert

mount

tubs

that

side

pointing upwards).

the

as

remove all dust caps and

the

standard observation

top

of

the microscope stand to posi-

(Fig.

3)

the

two

clamping levers on the right

of

the

dovetail mount are unclamped

tube

dovetail slide into the dovetail

on

the

microscope

far

as

pmibte.

-

1

)

2)

tube

stand and lower the

be

careful not to

-

from

I

1

touch

the optical elements

I-.,

Fig.

1

Fig.

2

-<

-

.

-.

4)

Firmly lock the
lever.

4.

Attaeh

1

2)

5.

Mount objectives and eyepieces.

the

)

Clamp the

edge

@

Clamp

tion tube in

revolving

the

vertical fluorescence

of

nosepiece.

tube

UV

protective

the vertical illuminator. (Fig.

vertical

illuminator to

the

same manner as the standard

with

shade

the

upper clamping

illuminator.

@

to the upper

the

4)

observa-

Fig.

Fig.

3

4

6.

Install the mercury burner.

Wipe the surface of the mercury burner clean with an alcohol%ther mixture, benzine, etc,

Use great

and when installing,

1)

Loosen the

in

Fig.

care

to make sure that no dirt, fingerprints, etc., are left on the bulb surface,

be

carefull not to touch the bulb portion.

socbt

5.

At this time, pay attention to the following:

clamping screw

0,

and lift up the socket

as

shown by the arrow

VI.

OPERATION

A.

Ignition of

(1) Be sure to

(2)

Note that the connecting cord prevents socket removal

2)

Remove

3)

Insert the lower electrode (marked with
with clamping nut
mounting terminal, and lock with clamping nut
burner envelope

the

use

a

DC

type mercury burner (HB0200W/2 or USH200MB).

the

retainer of the socket terminals, used for transportation.

0,

then insert the upper electrode into the slot of the upper

@

90'

away from the optical axis. (Fig.

Burner

unless

"+")

into the bottom terminal and tighten

@.

Be sure to turn the pearl on the

it

is

6)

Fig.

6

unplugged.

1)

Ascertain that the line voltage selector switch on the power supply unit is set to conform
with the local mains voltage. (This switch can

set to the following voltages: 100V-110V-120V or 220V-240V.)

2)

Ascertain that the freqnency selector switch on the power supply unit is set to conform
with the focal mains frequency (60Hz or 60Hz). If ypu find the switch is not correctly
positioned, unscrew the transparent cover and set the switch correctly.

3)

Check complete electric connection.

4)

Turneon the main switch of the power supply unit.

light.

5)

Press the start button, and the burner will ignite.

*

If

the line voltage is lower than

6)

Do not switch

*

Repeated on-off switching considerably shortens the burner life. After the burner is

switched

off,

off

the burner within

do

not re-ignite for 3 minutes or more in order

10%

of the rated voltage,

15

8

be

turned with a screwdriver, and can be

At

the

same

the

minutes after ignition.

time,

the pilot lamp will

arc will sometimes flicker.

to

give

it

time to cool.

-

Disconnect the power cord from

the power

B.

Burner Centration

After the arc has stabilized (2-4min.). center the burner as follows:

1) Rotate the exciter filter turret until the turret
click stops at the

2)

Open the shutter by pushing the shutter knob
all the

3)

Rotate the knurled rings

iris

iris

4)

swing' the

5)

Slide the dichroic mirror selector lever to the

"6"

6)

Insert the barrier filter 0-570 into the slot

supply

way.

diaphragm) and

diaphragm) to the

-

position.

unit.

"G"

position.

"f"

"Br"

@

MAX.

UV

protective shade to the left.

the

AC

@

(for the field
(for the aperture
position. (Fig.

*

\

outlet, and remove the fuse holder from

1

I

7)

of

the vertical illuminator.

Fig.

7

7) Place the burner centering mirror on the stage, and focus

8)

Pull out the light path selector lever built-in the observation tube
(yellow-green line).

9)

Remove the

As

tube.
of brightness) at the back of the objective.

NOTE:

If

reflected
manipulating the reflector centering knobs, because the reflected image moves

you

a) Bring the real image into focus with the collector lens focusing knob.

b)

c)

d)

e) Superimpose the real and reflected images.

*

cap

from

the photo eyepiece attachment hole on top of

you look through the opening, you can

the burner

operate the reflector centering knobs, while the real image does not.

Bring the real image to position
knobs.

Bring the real

by means of the reflector centering knobs. (Fig.

Equalize the sizes of the reflected and real
knobs.

As

a

is

out of center, four spots of brightness

images.

rule, this procedure

The reflected image can

and

reflected images into symmertrical positions with each other

is

required only after burner replacement.

see

be

(Fig.

8b) by means

the arc images of the burner (spots

identified from the real image,

8c)

images

on

it

with the

up

can

be

of

with the reflector focusing

10X

objective.

to the

CV

position

the

obsemtion

seen

as

the

real

the burner centering

and

by

as

w

Reflscted

image

(b)

Fig.

8

C.

FEuoresoence

1

)

Bring the area
mitted
manual

2)

Switch

Microscopy

of

the specimen to

light,

emitted

for

the

Universal Research

off

the transmitted light

from

exciter filter and dichroic mirror for

be

observed

the halogen

Microscope

socrrm,

your

into

the field of

or

tungsten filament

Model

and

select

the

specimen.

view,

and

focus

bulb.

(See

the instruction

VANOX-AHB-LB.

most suitable combination

with trans-

of

NOTE:

3)

4)

*

5)

Additional exciter filters in mount
used

as

desired. For details read

Stop

down

the field iris diaphragm

Stop down the aperture

Use

non-fluorescing

1OOX

After

gauze

(immersion

use,

moistened with xylene (no alcohol

carefully wipe

silicone

type)

Never leave immersion liquid

the

seriously impair

6)

The objectives

performance

UVFL40X

vided with iris diaphragms. It

to

increase contrast

7)

The

objective

spherically

glass.

For use

correct

of

the correction collar,

and

UVFC40X

for a thinner or thicker cover glass,

while looking through the

be

seen in

best

definition.

and

iris

diaphragm

immersion

objectives.

off

the

on

the

of

{immersion

is

recommended

barrier fitters in

E.

"Use of Filters".

("Fa')

until it is within the field

("A")

to ensure proper contrast.

oil

for

UVFL4OX

immersion liquid deposited on

or

ether should

lens

the

objective.

type)

surfaces

and

after

UVFL

to

stop

slide,

both provided,

of

(immersion type)

the

be

used,)

use

as

remnants of

lOOX

down

(immersion

the iris diaphragm slightly

image definition.

(dry) is provided with a correction collar which

as

well

as

a

0.1

set

it

at

microscope

and

0.17rnm

focusing

and

then

turn it in either direction

on

the specimen unti

view.

lens

the

type)

7mm

t

the

can

and

UVFL

surfaces

liquid will

are

can

be

set to

thick cover

image

be

with

pro-

can

8)

D.

Burner

1

2)

3)

When

fluorescence

it

is

good practice

to

turn

off

useful life

the mercury burner, since repeated on-off switching considerably shortens the

of

Replacement

)

The

average

be

kept

longer

It

is recommended to

at

the

end

of

Do

not

touch

the

main switch

observation

to

cut off

the

the burner.

life

of

burner

than

-2

hours.

keep

is

about

a

its life expectancy.

the burner

is

off,

for

and disconnect the lower connecting

is

to

beam

record

about

be

interrupted briefly

of

light

by

means

400

hours, provided

of

the

operating time of

10

minutes after switching

of

that

(for

the

each

cord

about

30

minutes or less),

opaque

shutter rather than

lighting duration should

each

off.

from

burner,

Then,

and

ascertain

the

lamp housing.

replace

it

that

E.

Use

of

Filters

Excitation
method

Ultraviolet

Violet

Blue

Green

Excitation methods

1)

Ultraviolets:

Exciter filter

and

applications

Exciter filter

turret

(UG-1)

U

V

{BG-3)

B

(BG-12)
None
G

(

I

F-545

BG-36)

Spectral
band

Wide
Narrow

Wide

Narrow

Wide

Narrow
Narrow

The line spectrum at bright lines 334nm and 365nm.

Fluorescence antibody method

Congo red test

+

(F

ITC)

Exciter filter

in mount
None

UG-1

UG-5

I F-405

BG-12

I

F-490

---

None

(2

pcs.)

Dichroic mirror
selector knob

U

(DM

400

+

L-420)

V

(DM

455

+

Y-455)

B

(DM

500

+

0-51

5)

G

(DM 580

0-590)

+

Barrier filter
in mount

L-420 and

UP

Y-475

and

UP

0-530

and

UP

R-610

2)

Violet: The line spectrum at bright lines 405nm and 435nm.

Catecholamine

3)

Blue:

Fluorescence antibody method

Acridine yellow and acridine orange

Auramine

Tetracycline

4)

Green: The line spectrum

Fluorescence antibody (TRITC)

Feulgen

Rhodamine

The line spectrum at bright lines 405nm
trum at 490nm.

B

(FITC)

at

bright line 546nm.

and

435nm,

and continuous spec-

Fuchsin

VII. OPTICAL DATA

Magnif ieation

'OX

20X

*40X

UVFL

(immersion

40X

type)

1 OOX

(immersion

type)

Focal length (mm)

Total magnif.

N

K5X

(Field

21

No.

WHK'OX

(20)

WHK1

5X

(14)

*

The resolving power

**

Optionally available.

Focal depth

.

Field
Total magnif.
Focal depth
Field of
Total magnif.
Focal depth

Field

of

of

view

view

view

0.4

1.16

15.84

0.84

50X

(w)

(mm)

(MI

(mm)

(p)

(mm)

is

28.83

2.1

lWX

15.7

2.0

150X

11.33

1.4

obtained with fully opened aperture diaphragm.

0.65

1.03

8.1 1

0.52

lOOX

9-05

1.05

2WX

5.02

.O

1

300X

3.67

0.7

0.85

0.25

4.59

0.395

Collection

collar

200X

3.66

0.53
4WX

2.1 2

0.5
600X

1.60

0.35

lris diaphragm

200X

4WX

600X

1

.30

0.1

4.56

0.26

2.28

0.53

1.25

0.5

0.92

0.35

1.30

1

0.14

1.91

0.26

lris diaphragm

500X

1.05

0.21

1,OOOX

0.65

0.2

1,500X

0.51

0.14

L

Technical terms:

Working distance:

(W.

D)

Numerical aperture:

A.)

(N.

Resolving power:

Focal depth:

Field number:

Field

of

view diameter: The actual size of the field of view in mm

The distance from cover glass to the closest point

of

tive when focused on the specirnan.

N.

A.

The

represents a performance number which

pared to the relative aperture (f-number)

N.

A.

values can

be

used for directly comparing the resolutions of

all types of objectives. The larger the N.

of

a camera lens. The

A.,

the higher the resolv-

can

ing power.
The

ability of a lens to register small details. The resolving power

of a lens

The distance between the upper and lower limits of
the image formed by an optical system.

aperture iris diaphragm, the focal
the

A

number that represents the diameter

field diaphragm that

is

measured

N.

A.

of an objective, the shallower

by

is

formed

its

ability to separate two points.

sharpness

As

you stop down the

depth

becomes

the

in

by

the lens in front of

larger, The larger

focal depth.

mm of the image of the

it.

on

the object surface.

the

objec-

be com-

in

VIII.

SPECTRAL

1.

Barrier filters

2.

Ultraviolet exciter filters

CHARACTERISTICS

in

slide

m

OF

FILTERS

If

400

Ill

I"'"'

IJ

III

WO

700

War.len#ll~

am

181)

3.

Violet exciter filters

4.

Blue

exciter

5.

Green exciter

filters

filters

*

+--&q

.

-

"

i

OLYMPUS

7

P

v;

i

h

*

I.

.

-1.

'

r

OPTIC

,

CO.,

-7

C-

LTC).

1

12-2,

WISHISHINIUKU I -CHOME,

SHINJUKU-KU

TOKYO,

JAPAN.