
CUV-ALL 4-Way Cuvette Holder
Installation Instructions
The CUV-ALL 4-way cuvette holder for 1-cm path length cuvettes has four collimating lenses that
couple to optical fibers, light sources, and spectrometers to measure absorbance, fluorescence,
scattering, or any combination of these optical phenomena.
Parts Included
CUV-ALL assembly for holding 1-cm cuvettes
Black cover for eliminating ambient light
Two barbed fittings for connecting a temperature stabilizing water source
Screwdriver for adjusting the fit of the cuvette
Allen wrench for adjusting the collimating lenses
043-00000-UV-01-201010 1

CUV-ALL Cuvette Holders Installation Instructions
Installation
Follow the installation instructions that correspond to your type of measurement.
Attaching Fibers for Absorbance Measurements
For UV Measurements
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV
label and a red lens cap.
2. Attach the other end of this fiber (the illumination fiber) to a UV light source.
3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first
fiber. This fiber also has a red lens cap.
4. Attach the other end of this fiber to the spectrometer.
For VIS Measurements
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the
VIS label and a black lens cap.
2. Attach the other end of this fiber (the illumination fiber) to a light source.
3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first
fiber. This fiber also has a black lens cap.
4. Attach the other end of this fiber to the spectrometer.
Attaching Fibers for Fluorescence Measurements
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV
label and a red lens cap.
2. Attach the other end of this fiber to a light source or an excitation source. Typically for
fluorescence measurements, the illumination fiber connects to a UV lens.
3. Attach another SMA-terminated optical fiber to the collimating lens immediately to the left or
right of the first fiber. The two collimators must be positioned at a 90° angle for fluorescence
measurements.
4. Attach the other end of this fiber to the spectrometer. Typically for fluorescence
measurements, the read fiber connects to a VIS lens.
2 043-00000-UV-01-201010