CUV-ALL 4-Way Cuvette Holder
Installation Instructions
The CUV-ALL 4-way cuvette holder for 1-cm path length cuvettes has four collimating lenses that
couple to optical fibers, light sources, and spectrometers to measure absorbance, fluorescence,
scattering, or any combination of these optical phenomena.
Parts Included
CUV-ALL assembly for holding 1-cm cuvettes
Black cover for eliminating ambient light
Two barbed fittings for connecting a temperature stabilizing water source
Screwdriver for adjusting the fit of the cuvette
Allen wrench for adjusting the collimating lenses
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CUV-ALL Cuvette Holders Installation Instructions
Installation
Follow the installation instructions that correspond to your type of measurement.
Attaching Fibers for Absorbance Measurements
For UV Measurements
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV
label and a red lens cap.
2. Attach the other end of this fiber (the illumination fiber) to a UV light source.
3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first
fiber. This fiber also has a red lens cap.
4. Attach the other end of this fiber to the spectrometer.
For VIS Measurements
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the
VIS label and a black lens cap.
2. Attach the other end of this fiber (the illumination fiber) to a light source.
3. Attach another SMA-terminated optical fiber to a collimating lens directly opposite of the first
fiber. This fiber also has a black lens cap.
4. Attach the other end of this fiber to the spectrometer.
Attaching Fibers for Fluorescence Measurements
► Procedure
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses with the UV
label and a red lens cap.
2. Attach the other end of this fiber to a light source or an excitation source. Typically for
fluorescence measurements, the illumination fiber connects to a UV lens.
3. Attach another SMA-terminated optical fiber to the collimating lens immediately to the left or
right of the first fiber. The two collimators must be positioned at a 90° angle for fluorescence
measurements.
4. Attach the other end of this fiber to the spectrometer. Typically for fluorescence
measurements, the read fiber connects to a VIS lens.
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CUV-VIS, CUV-UV Cuvette Holders Installation Instructions
Installing the Fiber Supports
► Procedure
1. Snap the clamps around the fibers after the fibers are screwed into the light source and
spectrometer.
2. Lift the clamps until they support the fibers.
3. Unsnap the clamps to remove the fibers.
Adjusting the Fit of the Cuvette
The CUV-ALL is preset for use with a 1-cm quartz cuvette. When properly adjusted, the cuvette
should fit snugly in the holder.
► Procedure
1. Locate the two ball plunger screws. They are under two of the four collimating lenses.
2. Remove the fiber clamps for easier access to the screws.
3. Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the
screws is just visible in the cell holder.
4. Insert your cuvette into the holder.
5. Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress. Do
not over tighten.
Installing Filters
► Procedure
1. Loosen the filter clamping screw with the provided screwdriver.
2. Insert the filter into the filter slot. The filter slot can accommodate filters up to 6 mm thick.
3. Clamp the filter in place by gently tightening the clamping screw finger tight.
Using the Temperature Stabilization Feature
This feature is used to heat or cool the cuvette holder base and cuvette.
► Procedure
1. Remove the two plugs from the top side of the base. (The plug on the right side of the base
should stay in place but might require thread tape.)
2. Replace the plugs with the two barbed fittings (or any 1/8” NPT pipe thread fittings). Thread
tape might be required on the fittings.
3. Connect the fittings to a water source. Water will circulate through the base.
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CUV-ALL Cuvette Holders Installation Instructions
Specifications
Specification Value
Path length 1 cm
Collimating lenses (VIS-NIR)
Collimating lenses (UV-VIS-NIR)
Collimating lens termination SMA 905
Fluorescence option Position 2 collimators at 90°
Filter slot accepts filters up to ¼" (6 mm) in thickness
Base material aluminum
Water input fittings
* Though the VIS-NIR lens is optimized for use to 2 m, it can be configured to "see" only to 1100 nm with
an Ocean Optics spectrometer.
BK 7 glass (~360 nm – 2
Dynasil 1100 quartz (200 nm-2
diameter, f/2
1/8” NPT pipe thread
m*), 5 mm diameter, f/2
m), 5 mm
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