Nuaire NU-677-400E, NU-677-500E, NU-677-600E User guide

LABGARD® ES Energy Saver Class II,

Animal Handling

Biosafety Cabinet

Models

NU-677-400E/500E/600E

Console

Operation & Maintenance Manual

November, 2021

Revision 1

Manufactured by:

NuAire, Inc.

2100 Fernbrook Lane

Plymouth, Minnesota USA 55447

Toll Free: 1-800-328-3352

In MN: 763-553-1270

Fax: [phone]

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Congratulations!

www.hc-sc.gc.ca

www.cdc.gov/od/ohs/

www.absa.org

www.absa-canada.org

www.ebsa.be

www.inspection.gc.ca

www.who.int

www.biosafety.be

www.hse.gov.uk

www.nsf.org

www.cetainternational.org

www.osha.gov/dts/osta/

www.nuaire.com

You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care,
maintenance (certification), and laboratory procedure, this cabinet will give you years of product and personnel
protection from particulate contaminants as prescribed in NSF/ANSI 49. Please read this manual carefully to familiarize
yourself with proper installation, maintenance and operation of the cabinet.

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ABOUT THIS OPERATION & MAINTENANCE MANUAL

The information contained in this manual is intended to reflect our current production standard configuration
model along with the more frequently purchased options. Any unique additions/modifications/shop drawings are
appended in the back flap of this manual, along with any modifications and/or additions to procedures as outlined in this
manual. A copy of the original factory test report is also appended to this manual. In case this manual and/or test
report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be obtained by calling or writing
NuAire, Inc. stating the model number and serial number and a brief description of the information desired.

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LABGARD® ES Energy Saver Class II,

Animal Handling Biosafety Cabinet

Models NU-677-400E/500E/600E

Operation & Maintenance Manual

TABLE OF CONTENTS

Section No. 1 ........................................................ General Description

Section No. 2 ........................................................ Models & Features

Section No. 3 ........................................................ Warranty

Section No. 4 ........................................................ Shipments

Section No. 5 ........................................................ Installation Instructions

5.1 ................................................................... Location

5.2 ................................................................... Set-up Instructions

5.3 ................................................................... Certification Testing Methods and Equipment

Section No. 6 ........................................................ Operating the NU-677E

6.1 ................................................................... Operator Controls & Indicators

6.2 ................................................................... Operating Guidelines

6.3 ................................................................... Operating Sequence

6.4 ................................................................... Ergonomics

6.5 ................................................................... Cleaning Procedure

Section No. 7 ........................................................ General Maintenance

7.1 ................................................................... Decontamination

7.2 ................................................................... LED Lamp Bulb Replacement

7.3 ................................................................... HEPA Filter/Motor Replacement

7.4 ................................................................... Sliding Window Replacement & Adjustment

7.5 ................................................................... Airflow Calibration

7.6 ................................................................... HEPA Filter Leak Test

7.7 ................................................................... Airflow Smoke Pattern Test

7.8 ................................................................... Site Installation Assessment Tests

7.9 ................................................................... Main Control Board Description & Replacement

Section No. 8 ........................................................ Error Indicators, Troubleshooting, Option-Diagnostics and Airflow

8.1 ................................................................... Error Indicators

8.2 ................................................................... Option Parameters

Section No. 9 ........................................................ Remote Contacts

Section No. 10 ...................................................... Optional Equipment

10.1 ................................................................. Ultraviolet Light

Section No. 11 ...................................................... Electrical/Environmental Requirements

Section No. 12 ...................................................... Disposal and Recycle

Insert .................................................................... Replacement Parts

MANUAL DRAWINGS

ACD-15280 .................................................... NU-677 Airflow Schematic

BCD-19494 .................................................... NU-677-400E Specification Drawing

BCD-19495 .................................................... NU-677-500E Specification Drawing

BCD-19496 .................................................... NU-677-600E Specification Drawing

BCD-16521 .................................................... Front Panel

ASSEMBLY DRAWINGS

BCD-16301 .................................................... Control Center & Front Decorative Panel Assembly

BCD-16376 .................................................... Sliding Window Assembly & Adjustment

BCD-16299 .................................................... Base Cabinet Assembly

ELECTRICAL SCHEMATICS

CD-000080..................................................... NU-677-400E/500E/600E Electrical Schematic

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Sensor Performance Verifications

NuAire, Inc. - Plymouth, Minnesota, U.S.A.

1.0 General Description

1.1 Description

The LABGARD® ES Model NU-677E Animal Handling Biosafety Cabinet (LFBSC) is a bench/table top model, with
an integral castored support stand for operation as a console model. The LABGARD® ES model NU-677E utilizes
an Energy Saver DC ECM motor optimally determined forward curved fan for each model size/width to maximize
both energy efficiency and filter loading capacity. The Energy Saver ECM motor is controlled via a solid-state DC
motor controller that provides automatic compensation (constant volume control) for both filter loading and
line voltage variances.

The Laminar Flow Biosafety Cabinet, (LFBSC) is a product resulting from the development of the "laminar flow"
principle (see airflow schematic) and the application of environmental controls as required in the field of
biological research or chemical containment. The LFBSC, when used with proper technique, is an effective
laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure
of both product and personnel to airborne biological or particulate chemical agents in low to moderate risk­hazard research and drug preparation or product operations, as prescribed by the Center for Disease Control
(CDC) Atlanta, Georgia.

The NU-677E bench LFBSC is known as a Class II, Biosafety Cabinet. This is possible since NuAire's cabinet
conforms to the following requirements:

LABGARD® ES Energy Saver Class II,

Animal Handling Biosafety Cabinet

Models

NU-677-400E/500E/600E

MANUFACTURED BY:

- Maintain a minimum average s inflow velocity of 100 ft/min (0.51 m/s) through the work access

opening;

- Have HEPA/ULPA filtered downflow air that is a portion of the mixed downflow and inflow air from a

common exhaust plenum;

- May exhaust HEPA/ULPA filtered air back into the laboratory or to the environment through an external

exhaust system connected to the cabinet with a canopy connection; and

- Have all biologically contaminated ducts and plenums under negative pressure or surrounded by

negative pressure ducts and plenums.

If working with volatile chemicals, the unit must be connected to an external exhaust system. Cabinets may be
used for work with volatile chemicals if permitted by a chemical risk assessment.

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1.2 Safety Instructions

NOTE:

CAUTION used without the safety alert symbol

not avoided, may result in property damage.

Potential electrical hazard, only qualified person
to access.

Used for important information.

Biohazard

Ground, Earth

CAUTION



Lead Free

Chemical Hazard
Flammable Hazard

Hazardous Gases! Personal

Safety alert symbol indicates a potentially

result in death of serious injury.

Safety alert symbol indicates a potentially hazardous situation which,

WARNING

!

CAUTION

!

These safety instructions describe the safety features of the LABGARD® ES Model NU-677E LFBSC.

The safety cabinet has been manufactured using the latest technological developments and has been thoroughly
tested before delivery. It may, however, present potential hazards if it is not used according to the intended
purpose or outside of operating parameters. Therefore, the following procedures must always be observed:

• The safety cabinet must be operated only by trained and authorized personnel.

• For any operation of this unit, the operator must prepare clear and concise written instructions for

operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical
regulations, in particular.

o which decontamination measures are to be applied for the cabinet and accessories
o which protective measures apply while specific agents are used
o which measures are to be taken in the case of an accident

• Repairs to the device must be carried out only by trained and authorized expert personnel.

• Keep these operating instructions close to the unit so that safety instructions and important information

are always accessible.

• Should you encounter problems that are not detailed adequately in the operating instructions, please

contact your NuAire Representative of NuAire technical Services.

1.3 Explanation of Symbols

hazardous situation which, if not avoided, could

if not avoided, may result in minor or moderate injury.

indicates a potentially hazardous situation which, if

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Protection Equipment Required.

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2.0 Models & Features

The model NU-677E, LABGARD® ES Class II Laminar Flow Biosafety Cabinet is manufactured in three sizes: 4 ft.,
5 ft., and 6 ft.

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3.0 Warranty

Details regarding product warranties can be found in the published warranty data separate from this manual

and included within the data packet sent with the unit.

4.0 Shipments

NuAire takes every reasonable precaution to assure that your LABGARD® ES cabinet arrives without damage.
Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase.
However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a
NuAire LABGARD® ES cabinet to be sure that if damage has occurred, the proper claims and actions are taken
immediately.

4.1 Damaged Shipments

4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before

acceptance.

4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the
consignee's copy of the freight bill. Then an inspection must be made to verify the claim against the
carrier. This inspection is the basis of your filing the claim against the carrier.

4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and
request an inspection. Without this inspection, the transportation company may not accept a claim for
loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that
he was contacted on a certain date and that he failed to comply with the request. This along with other
papers in the customer's possession will support the claim.

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5.0 Installation Instructions



5.1 Location

Within the laboratory, pharmacy, etc., the ideal location of the Biosafety cabinet is away from personnel traffic
lanes, air vents (in or out), doors and/or any other source of disruptive air currents.

If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening,
the potential exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the
severity of the air current. Since this cabinet is mobile, disruptive air currents should be evaluated in each
potential cabinet location.

REMEMBER: A BIOSAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.

Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for maintenance
purposes. The electrical outlet into which the cabinet is connected should be readily accessible for maintenance
purposes. Do not position the cabinet to prevent access to the power cord. The power cord plug serves as the
disconnect and should remain readily accessible. If the outlet is inaccessible, such as a conduit (hardwired)
connection, then an appropriate warning label should be applied near the cabinets on/off switch to indicate the
circuit breaker on the power distribution panel to be used. A MINIMUM CLEARANCE OF 6" (152MM) IS
REQUIRED FROM THE TOP OF THE CABINET TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST
EFFLUX.

In addition, if this cabinet is used in microbiological application with volatile chemicals and radionuclides, Per
CDC/NIH and NSF it is strongly recommended that the cabinet be exhausted to the outside. NuAire offers a
canopy type of exhaust transition, which will capture the exhaust efflux from the cabinet.

NOTE: THE EXHAUST SYSTEM SHOULD BE FITTED WITH A BACKDRAFT
DAMPER TO PREVENT REVERSING OF AIRFLOW IN THE SYSTEM.

NuAire strongly recommends a canopy or thimble exhaust for most applications. See separate instruction

sheets for a discussion of exhaust transitions and installation requirements.

5.2 Set-Up Instructions

Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually
the best procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate
ease in handling. It can then be removed from the skid by removing the banding, bolts and screws holding the
cabinet to the skid. The castors may then be attached. The cabinet should be pushed off the skid attaching the
castors on each corner as the castor base plates hang over the skid. IT IS RECOMMENDED THAT NO LESS THAN
TWO PEOPLE PERFORM THE SKID REMOVAL AND CASTOR ATTACHMENT PROCESS.

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5.2.1 Gas Service

CAUTION

!

NuAire doesn't recommend the use of natural gas within the BSC, but if gas service is determined to be
necessary for the application, appropriate safety measures must take place. All NuAire BSC's have
precautionary warning labels that say the following:

Use of explosive or flammable substances in this cabinet

should be evaluated by your appropriate safety personnel.

Once the determination has been made by the appropriate safety personnel, the application of natural
gas must be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY

RECOMMENDED THAT AN EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE BSC ON
THE GAS SUPPLY LINE.

All NuAire BSC's meet the safety requirements of UL and CSA for Laboratory Equipment. To comply with
these safety requirements, NuAire uses only certified gas valves. In addition, if external piping is
required, only black pipe is used for this application.

As previously stated; NuAire doesn't recommend the use of natural gas within the BSC and ASSUMES
NO RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the BSC
not only contributes to heat build-up; is also disrupts the laminar air stream, which must be maintained
for maximum efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON

DEMAND IGNITION IS STRONGLY RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS.

During use, the Bunsen burner should be placed to the rear of the workspace where resulting air
turbulence will have a minimal effect.

5.2.2 Plumbing Services
Service ball valves with the type of service specified by the removable button on the handle are located
in the work zone. The service ball valves are not recommended for pressure over 75 p.s.i. (5.2 BAR).
Reducing valves should be installed external to the cabinet if necessary. Service ball valves should never
be used for flammable gasses or oxygen service. A special needle valve for oxygen service or certified
valve is required and available upon request.

External connection is to 3/8 inch NPT coupling in the inner sidewalls. Connection to plant utilities
should be made with proper materials for the individual service and according to National and/or Local
codes. Observe all labels pertaining to the type of service and operating pressure.

5.2.3 Electrical Services

The NU-677E series Laminar Flow Biosafety Cabinets may be "hardwired" (optional) or connected via an
electrical power cord which is standard. The unit requires 230 VAC, 50/60 Hz, single phase (current
rating varies per cabinet size, reference Electrical/Environmental Requirements). It is recommended
that power to the unit be on its own branch circuit, protected with a circuit breaker or fuse at the
distribution panel. A surge protector is strongly recommended if you are experiencing power related
faults.

PLEASE NOTE: THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE LIGHTING. ELECTRONIC BALLASTS
OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH
GROUND FAULT CIRCUIT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO
TRIP.

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5.2.4 Final Assembly



REMOVE THE PROTECTIVE CARDBOARD COVER OVER THE EXHAUST HEPA FILTER, located under the
protective screen if in place. The exterior surface and viewing glass are easily cleaned with any mild
household detergent cleaner using a soft cloth. Harsh chemicals, solvent-type cleaners and abrasive
cleaners should not be used.

Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned
with any mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for
60 minutes before using it as a LFBSC.

5.3 Certification Testing Methods and Equipment

After installation and prior to use, NuAire recommends that the cabinet be certified or commissioned to factory
standards. At a minimum, the following tests should be performed.

1. HEPA filter leak test

2. Downflow velocity test

3. Inflow velocity test

4. Airflow smoke patterns*

5. Site installation assessment tests

The testing methods and equipment required are specified on the factory inspection report included with this
manual (see insert in back cover).

IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR
WITH THE METHODS AND PROCEDURES FOR CERTIFYING BIOSAFETY CABINETS (SEE INSERT).

AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A
MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME
THE OPERATOR FEELS IT IS NECESSARY.

Note that the LABGARD® ES cabinets, filters and seals provide premium performance; Quality Control in both
design and manufacturing assure superior reliability. However, protection to both product and operator is so
vital that certification to the performance requirements should be accomplished as stated to ensure Biosafety
established by the factory standards.

Note: If using BSC in more than one location, follow the NSF/ANSI 49 guidelines below.

* NSF/ANSI 49 Annex E.3.3.6 Mobile Installations

Mobile Base stands with and without lift capability have been used when the BSC is operated in multiple

locations in the same or adjoining laboratories. Proper cabinet operation should be confirmed by airflow smoke
pattern tests at each site of use. If the cabinet is relocated to another facility, or subjected to excessive shock
and/or vibration during moving, the BSC should be recertified to ensure it is functioning in a proper manner.

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LABGARD® ES Energy Saver Class II,

Catalog Number

NU-677-400E

NU-677-500E

NU-677-600E

Performance Specifications

2. Product Protection

EN12469

EN12469

EN12469

NSF Std. No. 49 Class

Class II

Class II

Class II

Style of Cabinet

Bench top/console
w/basestand

Bench top/console
w/basestand

Bench top/console
w/basestand

Cabinet Construction

All welded stainless steel

tight design

All welded stainless steel

tight design

All welded stainless steel

tight design

Diffuser for Air Supply (Metal)

Non-flammable

Non-flammable

Non-flammable

HEPA Filter Seal Type:

Exhaust Filter-99.99% Eff. on 0.3 microns

Neoprene, Spring-loaded

Neoprene, Spring-loaded

Neoprene, Spring-loaded

Fumigation per NIH/NSF Procedure

Yes

Yes

Yes

Standard Services: Outlet

Two, Backwall

Two, Backwall

Two, Backwall

Optional Services:

Ultraviolet Light

One, Backwall

One, Backwall

One, Backwall

Cabinet Size Inches (mm):

Depth (with Control Center and Armrest)

32-1/8 (817)

32-1/8 (817)

32-1/8 (817)

Work Access Opening Inches (mm):

Work surface Height Min/Max

24-1/2 (662) / 43 (1092)

24-1/2 (662) / 43 (1092)

24-1/2 (662) / 43 (1092)

Work Zone Inches (mm):

Window Height

Viewing Window Inches (mm):
Standard is tempered sliding glass

Fully closed to
21 (533) open

Fully closed to
21 (533) open

Fully closed to
21 (533) open

Required Exhaust CFM/CMH Standard/

Fixed Flow Canopy (NU-907)

526 (894)

651 (1106)

796 (1352)

Plant Duct Static Pressure Eng./Metric

0.05-0.1"/1.27-2.54mm

0.05-0.1"/1.27-2.54mm

0.05-0.1"/1.27-2.54mm

Heat Rejected, BTU, Per Hour (non-

(vented)

2551

2669

2787

Electrical: (CE)

12 ft. Power Cord (one)

16A

16A

16A

* Crated Shipping Weight:
Net Weight

800 lbs. /362 kg.
750 lbs. /340 kg.

865 lbs. /392 kg.
815 lbs. /369 kg.

930 lbs. /421 kg.
880 lbs. /398 kg.

Sound Pressure Level per ISO 4871****

Not to exceed 58 dbA 

Not to exceed 60 dbA 

Not to exceed 62 dbA 

Animal Handling Biosafety Cabinet

Models NU-677-400E/500E/600E

Catalog Number

1. Personal Protection

Supply Filter-99.99% Eff. on 0.3 microns

Gas Cocks 3/8" NPT

Height Maximum
Height Minimum
Width

Nominal 4 foot (1.2m)

NSF/ANSI 49

16/18GA, Type 304 pressure

HEPEX Seal

Up to 2 ea. Sidewall

97-1/2 (2477)
79 (2007)
53-1/2 (1358)

Nominal 5 foot (1.5m)

NSF/ANSI 49

16/18GA, Type 304 pressure

HEPEX Seal

Up to 2 ea. Sidewall

97-1/2 (2477)
79 (2007)
65 1/2 (1663)

Nominal 6 foot (1.8m)

NSF/ANSI 49

16/18GA, Type 304 pressure

HEPEX Seal

Up to 2 ea. Sidewall

97-1/2 (2477)
79 (2007)
77 1/2 (1968)

Standard Opening Height/Optional
Standard Inflow Velocity

Height
Width
Depth Measured at 12 inches (305)

Optional:
Variable Flow Canopy (NU-911)

vented)

Volts, AC 50/60 Hz
+Amps: Blower/Lights/Autolift
Amps: Duplex
Amps: Total

12 (305)
105 FPM (.53 m/s)

28 1/2(724)
46 3/8 (1178)
25 1/2(642)

486-656 (626-1115)

157

230

4.5
3
11

12 (305)
105 FPM (.53 m/s)

28 1/2(724)
58 3/8 (1483)
25 1/2(642)

611-761 (1038-1293)

178

230

5.5
3
11

12 (305)
105 FPM (.53 m/s)

28 1/2(724)
70 3/8 (1788)
25 1/2(642)

758-868 (1288-1475)

198

230

5.8
3
11

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* Crated shipping weight does not include weight for accessories or options

+ Based on cabinet with new filters running at 230VAC

.

****Uncertainty is K = 2 dbA, measurement performed per ISO 11201 in normal running mode.

Figure 1

Reference the customer test report for procedure and results.

6.0 Operating the NU-677E

6.1 Aeromax™ Control System

6.1.1 Overview

The Aeromax™ control system is designed to service the control requirements of the LABGARD® ES NU­677E Biosafety Cabinet. The Aeromax™ control system consists of an electronic module that will
perform the following functions:

• Easy user interface via LED’s and function keys

• Control blower via solid state switch.

• Control lights via solid state switch.

• Control outlets via solid state switch.

• Disable audible alarm switch with ring back function.

• Control blower DC ECM motor with solid-state DC Motor Controller that provides automatic

compensation (constant volume control) for both filter loading and line voltage variances.

• Monitor and display airflow system performance via PresurFlow™ monitor.

The LabGard® ES NU-677E offers the latest digital microprocessor design technology for improved
cabinet performance and safety. The Aeromax™ control system integrates a digital pressure sensor
(PresurFlow™) to monitor the cabinet’s airflow performance. The Aeromax™ control system also
integrates a DC motor controller that provides automatic compensation for both filter loading and line
voltage variances. There is additional on/off control of blower, LEDLED light, ultraviolet light (optional)
and outlets. Lastly the Aeromax™ control system monitors the sliding window position with micro
switches. All the above functions are shown in a system block diagram (see figure 1).

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6.1.2 Front Panel

The control system front panel contains the following functions described in detail (see Drawing BCD-

16521).

6.1.2.1 Blower Keys

The blower key controls the ON/OFF power to the blower.
LED above key indicates : full green for blower on, blinking green for blower pending and
full red for blower alarm.

6.1.2.2 Hidden Key
The hidden key is located just above the blower LED indicator centered in the airflow symbol. The

hidden key is used for various functions including the blower password 3 key sequence if the option
is activated.

6.1.2.3 LEDLED Light Key
The LED light key controls the on/off power to the LED light.

LED above the key indicates full blue for LED light on.

6.1.2.4 Ultraviolet (UV) Light Key
The UV light key controls the on/off power to the UV light (if optionally installed).

LED above indicates full yellow for UV light on.

6.1.2.5 Outlet Keys

The outlet key controls the ON/OFF power to the outlets.
LED above indicates full blue for outlets on.

6.1.2.6 Red Alarm LED

The red alarm LED will indicate any alarm condition and remain indicating until the alarm condition

is cleared.

6.1.2.7 Audible Alarm Silence

The audible alarm silence key allows user interaction to silence an audible alarm for a period of 15

minutes. After 15 minutes if the alarm condition still exists, the audible alarm will again sound.

The audible alarm silence key also is used to exit all FlowGard™ user interaction menus.

6.1.2.8 Arrow Adjustment Keys

The arrow adjustment keys allow user interaction for various functions.

6.1.3 Aeromax™ Control System Power

After the LabGard® ES NU-677E is plugged into the appropriate facility line power the control system will

power up. The control panel will also indicate the power up status by blinking the red alarm LED.
Pressing any key will acknowledge the power up status and turn off the blinking red alarm LED.

If a power interruption occurs, all control system functions, calibrations and parameters will be

maintained and continue upon restoration of power. Just as the initial power up, the red alarm LED will
blink to indicate power up status.

6.1.4 Standby Mode

When the BSC is not in use any of the function keys except the blower that initiates run mode may be
turned on and off in standby mode.

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6.1.5 Run Mode





Any time the blower run key is pressed with the sliding window at its correct operational height, the
RUN MODE screen will be initiated. The Run Mode will start with the PresurFlow™ entering and
approximate 3 minute warm up period. The PresurFlow™ LED indicators will blink and indicate the
following sequence:

st

• 1

minute – Left and right Red LED’s will blink

nd

• 2

• 3

minute – Left and right Green LED’s will blink

rd

minute – Center 3 Green LED’s will blink

Once the warm up period is complete, only one LED will indicate cabinet airflow status.

During the warm up period the aseptic cleaning process may begin. If the sliding window is raised, an
audible and visual alarm will occur, but may be silenced by pressing the alarm silence key.

6.1.6 Nite Care Mode

The NU-677E may be configured to allow the DC ECM motor to continue to run at a lower rate with the
sliding window closed allowing the workzone interior to be continually HEPA filtered. If the Nite Care
mode is configured, the blower must be on (green LED above blower key will blink) and the window
closed for it to be activated. Once activated, blower LED indicator will blink fast and the PresurFlow™
will indicate 3 Green LED indicators will blink. In addition, the LED light will be disabled. The UV light
may be turned on if installed.

6.1.7 Standby/Run Mode Alarms

If present, standby/run mode alarms will be both visual and audible, the red alarm LED oval will turn on.
Audible alarms will produce an alarm tone for 30 seconds, then ring back for 2 seconds of every 5
seconds. Pressing the alarm silence key will silence the audible alarm for 15 minutes initially then will
start the ring back function again.

The list below represents alarm types and their respective priority from the highest to lowest priority.

1) New Firmware Loaded

2) Internal Board Failure

3) Power on Reset

4) Airflow Pressure Alarm

5) Blower RPM Failure

6) Window High

7) Window Low

8) Replace UV Light

Note: The above messages are described in greater detail in section 8.

6.1.8 Operator Accessible Functions

6.1.8.1 Access and Navigation
To access the operator accessible functions,

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6.1.8.2 Auto Timer Duration

Auto timer duration timers are countdown timers for the functions displayed once time is entered
into a function. The timer will begin to countdown upon the start of that function (i.e. press UV light
key to start timing the UV light). The LED indicator above the function key will start to blink
indicating the timer function. If the LED indicator was full on, no timer function is present. As the
timer expires the function will turn off.

• Select auto timer duration function
o Outlets

LED in

o Lights

indicator above light will blink fast. Adjust desired time as described below.

LED

o UV Light

ED indicator above UV light will blink fast.

L
Adjust desired time as described below.

o Nite Care Blower

dicator above outlet will blink fast. Adjust desired time as described below.

L

ED indicator above Blower key will blink fast. Adjust desired time as described

below.

o Adjust countdown time

Press ↑ or ↓ keys to adjust time.
Time will change in 15 minute increments as shown on the PresurFlow™ LED
segments below.

15 minutes for Red LED 30 Minutes for Yellow LED

1 hour for green LED Represents 8 hours (maximum time)

• Press hidden key to accept time and exit.

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November/2021

6.1.8.3 Blower Password



The blower on/off password allows the cabinet user to place a 3 key sequence requirement to turn
the blower on or off.

The 3 key sequence for the blower password will be a combination of the hidden and blower keys.

• Select blower password

Re

d LED indicator above blower will blink fast.

• Select password

Press ↑ or ↓ key to scroll through the code choices below,

No Password

B-B-H password

B-H-B password (default)

B-H-H password only

H-B-B password

H-B-H password

H-H-B password

• Press hidden key to accept time and exit.

Note: If the required blower password option is selected in the blower airflow option menu (see

section 8.2.2). Then the “No password” choice above is not available and the default remains
B-H-B.

OM0329 Page 22 of 57
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

6.2 Operating Guidelines

The intent herein is to present general operational guidelines that will aid in the use of the Laminar Flow
Biosafety Cabinet (LFBSC) to control airborne contaminants of low to moderate risk as stated in Technical Report
No. FPS 56500000001 prepared by Dow Chemical U.S.A. for the National Cancer Institute, May 1, 1972.

Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be developed in
order to obtain a maximum potential for safety and protection. The pre-planning necessary to develop these
protocols is based on several fundamental considerations, each of which will contribute to optimum benefits
from the equipment:

a. Know your "safe working area"
b. Minimize disruption of "air curtain"
c. Minimize room activity
d. Utilize unidirectional air flow
e. Employ aseptic techniques

6.2.1 Know Your "Safe Working Area"
The LFBSC safe working area is basically the worktray or depressed area. All work should be performed on
or above the worktray. The area on or above the front grill is a non-safe working area.

6.2.2 Minimize Penetration of "Air Curtain"

The minimum number of items necessary should be placed into the cabinet to prevent overloading, but
the work should also be planned to minimize the number of times an operator's hands and arms must
enter and leave the air curtain at the open face. The ideal situation is to have everything needed for the
complete procedure placed in the hood before starting, so that nothing need pass in or out through the air
barrier at the face until the procedure is completed. This is especially important in working with moderate
risk agents.

Unnecessary raising of the hands inside the cabinet above the level of the work opening should be
avoided. This presents an inclined plane from hands to elbows along which the downflow of air may run
to, and possibly out, the open face.

Note: When working with agents of lower risk, it is not as important for all materials to be placed in

the cabinet before starting, or for the procedure to be completely finished before materials are
removed. Also, the time period for a unit may be continued over a more extended period
during which entries and withdrawals from the cabinet may be made.

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6.2.3 Minimize Room Activity

Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air
currents as well as interfere with the work of the operator. A person walking past the front of a cabinet
can cause draft velocities up to 175 fpm (.89 m/s), which are sufficient to disrupt the air balance of the
laminar flow unit.

6.2.4 Utilize Unidirectional Air Flow

The operator must keep two important facts in mind: (1) The air, as supplied to the work area through
filters from the top, is contaminant free and (2) Airborne contamination generated in the work area is
controlled by the unidirectional flow of parallel air streams in a top-to-bottom direction.

A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability
of controlling lateral movement of airborne particulates. A cone of turbulence extends below the object
and laminarity of the air stream is not regained until a point is reached downstream, approximately equal
to three to six times the diameter of the object. Within the parameters of this cone, particles may be
carried laterally by multidirectional eddy currents.

Transfer of viable materials and manipulations which may generate aerosols should not be performed
above sterile or uninoculated materials. Items should be localized on the work surface in "clean" and
"dirty" groups.

6.2.5 Employ Aseptic Technique

The operator must not assume an attitude of "let the cabinet do it" when performing procedures within a
LFBSC. Properly balanced and properly used cabinets will do an excellent job of controlling airborne
contamination and containing viable agents, but the cabinet will not eliminate contact transmission of
contamination. Normal laboratory contamination control procedures and basic aseptic techniques are
necessary to obtain maximum benefit from the cabinet. For example, open bottle, tube or flask mounts
should be kept as parallel as possible to the downflow to minimize capture of chance particulates. This
precaution is merely an extension of good aseptic technique as practiced on open bench tops. The good
laboratory practices designed to minimize creation and/or release of aerosols to the environment should
not be discontinued.

Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or
until surface-decontaminated. Trays of discard pipettes must be covered before removal from the cabinet
(aluminum foil may substitute for fabricated covers).

If an accident occurs which spills or splatters suspensions of etiologic agent around the work area, all
surfaces and items in the cabinet must be surface-decontaminated before being removed.

Applying a burner flame to flask and tube necks when mating surfaces of sterile assemblies is a
conventional method of minimizing chance contamination. However, the efficiency of this operation is
usually related to the removal of airborne contamination occurring while the item is uncovered. If the
manipulation is carried out in an environment free of airborne particulates, then the need for the flaming
operation is essentially removed. This is one of the additional advantages of the LFBSC - use of the gas
burner is seldom necessary.

The gas burner flame in one of these units not only contributes significantly to the heat build-up, it also
disrupts the laminar air streams which must be maintained for maximum efficiency. IF THE PROCEDURE

DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS RECOMMENDED. DO
NOT USE CONSTANT FLAME GAS BURNERS. It should also be placed to the rear of the work space where

resulting air turbulence will have a minimal effect. If cabinet air is inadvertently turned off, the flame could
damage the HEPA filters.

OM0329 Page 24 of 57
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6.3 Operating Sequence

6.3.1 Start Up

Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet to make sure they
are unobstructed.

Note: Some cabinets are equipped with ultraviolet (UV) lights. Good procedure includes the decontamination

or wipe down of cabinet surfaces with chemical disinfectant before work commences. This practice
eliminates the need for UV lights, whose primary utility in this application is inactivation of surface
contamination since the filters effectively remove all airborne contaminants. UV lights, therefore, are
not recommended in the LFBSC.

Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun in the
cabinet. If the filtered air exhausted from the unit is discharged into the room, as in some installations,
an additional advantage is obtained from purification (filtration) of the room air circulated through the
equipment. Because of this characteristic contributing to the quality of the laboratory environment,
some owners of LFBSC leave them in operation beyond the time of actual use.

6.3.2 Wipe down The interior surfaces of the work space should next be disinfected (see Cleaning Procedures) by wiping them thoroughly with 70% alcohol or similar non-corrosive anti-microbial agents. USE OF CHLORINATED

OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL.

6.3.3 Materials & Equipment

The apparatus and materials should next be placed into the cabinet. Care must be exercised that no items
be placed over the front intake grills. Materials should be arranged so that clean, dirty (used), and virus
materials are well separated. Passage of contaminated materials over uninoculated cultures or clean
glassware should be avoided and transfer of viable materials should be performed as deeply into the
cabinet (away from open face) as possible.

6.3.4 Air Purge Additional purging of the work space without user activity should be allowed for 2-3 minutes after materials and apparatus have been placed in it. This will rid the area of all "loose" contamination that may have been introduced with the items.

6.3.5 Perform Work

The work can now be performed. The technician performing the work is encouraged to wear a long­sleeved gown with knit cuffs and rubber gloves. This will minimize the shedding of skin flora into the work
area and concurrently protect the hands and arms from viable agent contamination. At a minimum, the
hands and arms should be washed well with germicidal soap before and after work in the cabinet.

OM0329 Page 25 of 57
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6.3.6 Terminal Purging & Wipe down

Following completion of work, allow the cabinet to run for 2-3 minute period without personnel activity to
purge the unit. The decontamination of the interior surfaces should be repeated after removal of all
materials, cultures, apparatus, etc. A careful check of grills and diffuser grids should be made for spilled or
splashed nutrients which may support fungus growth and resulting spore liberation that contaminates the
protected work environment.

6.3.7 Paper Catch/Prefilter

A permanent paper catch is installed behind the rear divider panel of the work zone. This area forms the
return air path to the motor/blower; and if the airflow is blocked, it could seriously affect the performance
of the cabinet. Therefore, THE PAPER CATCH SHOULD BE CHECKED AND CLEANED NO LESS THAN ON A
WEEKLY BASIS, DAILY BASIS if procedures dictate the use of paper products. Any paper removed must be
properly disposed of as Contaminated Hazardous Waste. The above procedure also applies to all units
configured with a prefilter.

6.3.8 Shut Down

Turn off blowers and lights. Do not use cabinet as a depository for excess lab equipment during periods of
non-operation. If antineoplastic agents are being prepared in the cabinet, it is recommended to let the
cabinet run 24 hours per day. This lessens the possibility that contaminants may escape.

6.4 Ergonomics

Ergonomics, the study or accommodation of work practices is extremely important for proper cabinet usage and
user health and safety. An evaluation of normal work practices should be performed with each user when

working in a cabinet. Evaluation criteria should be at a minimum:
a. Proper user posture
b. Effective workzone layout for work practice
c. Vision or sightlines

For each of the above evaluation criterion, several aids may be supplied to accommodate the user.

• Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to assure
proper user posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure
back is fully supported with proper chair adjustments.

• Forearm/armrest support - The cabinet is provided with a forearm support on the work access opening.
Periodic mini-breaks during work practice should be taken resting forearm to avoid stress and fatigue.

• Effective workzone layout - Always prepare your work procedure to minimize reach to avoid neck and
shoulder stress and fatigue. Rotating tables are optional to maximum workzone and minimize reach.

• Vision and sightline - Always prepare your work procedure to eliminate glare and bright reflections on
the window. Keep your window clean and sightlines clear to your effect workzone.

OM0329 Page 26 of 57
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6.5 Cleaning Procedures



6.5.1 General
Cleaning laboratory equipment is important in terms of both functionality and general good
housekeeping. The information provided below is intended to aid the development of facility Standard
Operating Procedures (SOP’s) for cleaning the equipment. It is strongly recommended that all cleaning
materials used be tested and verified in terms of both effectiveness and material compatibility before
they are written into the cleaning SOP documentation.

a. The airflow blower should be operating during the cleaning process to maintain sterility and/or
containment during the cleaning process.

b. Raise window to gain additional access if desired.

c. Apply appropriate cleaning material or surface disinfectant to surfaces. Most surface disinfectants
require a specific contact time depending the materials used within the work zone. CONSULT

APPROPRIATE DISINFECTANT DOCUMENTATION FOR PROPER APPLICATION AND SAFETY
PRECAUTIONS.

c-1. Stainless steel (type 304) has noted material compatibility concerns with Acids, Chlorides and
Halogens. IF THESE MATERIALS ARE USED AND ALLOWED TO BE LEFT ON THE STAINLESS STEEL
SURFACE, OXIDATION AND DEGRADATION WILL OCCUR. Only by re-wiping surfaces with either sterile
water or 70% IPA will remove harmful materials from the stainless steel surface.

Further information is available at the following:
content/uploads/downloads/2011/07/Parr_Stainless-Steels-Corrosion-Info.pdf

NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE
AT YOUR OWN RISK! The information provided above is from raw material suppliers and known general
source documents for use to develop application cleaning SOP’s.

NOTE: When cleaning the work area for the first several times, the new metal surfaces may produce
some dark discolorization on the white cleaning wipes. Repeated cleaning will continuously reduce the
amount of the discolorization material on the cleaning wipes over time.

1

Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributors.

2 American Society of Hospital Pharmacists. 1990. ASHP Guidelines on Handling Cytotoxic and Hazardous Drugs Am. J. Hosp. Pharm. 47:1033-4

http://www.parrinst.com/wp-

9

OM0329 Page 27 of 57
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7.0 General Maintenance

Cabinet Size

400E

500E

600E

Cabinet

Dimensions

58-7/8 x 28 x 46-3/8

(1.50 x .711 x 1.18 m)

58-7/8 x 28 x 58-3/8

(1.50 x .711 x 1.48m)

58-7/8 x 28 x 70-3/8

(1.50 x .739 x 1.88 m)

Cabinet

44.24 cu. ft.

55.69 cu. ft.

67.14 cu. ft.

CAUTION

!

CAUTION

!

CAUTION

!

All maintenance actions on this equipment must be performed by a
qualified technician who is familiar with the proper maintenance
procedures required including both certification and repair.

7.1 Decontamination

No maintenance should be performed on the interior of the LabGard® ES cabinet (area behind access panels)
unless the cabinet has been microbiologically decontaminated, is known to be biologically clean, or known to be
chemically inert. Surface disinfection is performed as specified in the Cleaning Procedures section.

Hazardous Gases! Personal Protection Equipment Required.

Decontamination using hazardous gas, vapor or mist must be performed in accordance with

the specifications of NSF/ANSI 49, Annex G, EN12469, Annex J or applicable national, state,
province or local regulations.

This procedure presents considerable risks and must be performed only by specially trained and authorized
service personnel in accordance with applicable national safety regulations. (i.e. US – OSHA/NIOSH, Germany
TRGS-522).

The decontaminate is generated either external or internal of the sealed cabinet. The quantity of
decontaminate should follow standard or manufacturer’s recommendations based on cabinet volume. The
decontaminate process should follow standards or manufacturer’s recommendations based on the
decontaminate used.

All decontaminate materials are hazardous (chemical-liquid, gas and

vapor) (Flammable – process) and are required to be handled properly. Follow
all product and process documentation and labelling.

7.1.1 Manual Decon

1. Disconnect power to the cabinet. Remove screws at each upper side of the control center and
allow the control center to rotate down, resting on the safety straps. Disconnect electrical
connectors on left side. Disconnect electrical from right side. (Be sure to note the location of
the supply and exhaust sensor wires before disconnecting them from the main board). Remove
control center by disconnecting safety straps lifting the control center up and away from
cabinet. Fold and pinch tubing to seal.

2. Remove the front decorative panel via top/front fasteners.

3. Remove window assembly (both frame and window, either being manual, sliding, or automatic)
via the upper and lower fasteners (Refer to BCD-16376). At this point, the sliding window
assembly can be removed.

4. Remove exhaust sensor shrouds.

5. Remove the diffuser screen and gasket around perimeter of workzone, if present.

6. Prepare decontamination equipment. Reference decontamination procedure and use the
following chart to calculate chemical requirements.

OM0329 Page 28 of 57
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Volume

(1.25 cu. m)

(1.58 cu. m)

(1.90 cu. m)



Note:

interior outlets and fan during the decontamination.

CAUTION

!

7. Set control center on its side resting on the left side of cabinet. Then plug in the 16-pin
connector cable from the cabinet to the control center. Reconnect power to the cabinet. The
outlets and blower can then be activated.

8. Seal front and top openings using plastic and tape.

BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY

LABORATORY EXPOSURE TO DECONTAMINATION GAS.

9. Perform decontamination procedure.

Please consult with NuAire, Inc. about any unique contamination problems.

Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area

behind the access panel containing the HEPA filters and motor (blower assembly). All required
adjustments in order to maintain proper cabinet airflows are external to the cabinet interior.
The motor is lubricated for life and is thermally protected with automatic reset.

The outlets in the work area are energized as long as the cabinet is plugged
in and switched on the front panel. Unplug the cabinet before
decontamination equipment is plugged into these outlets. The control
centers electrical connectors may be re-attached to utilize the cabinet's

7.2 LED Lamp Replacement

The two (T8) LED lamps are cool white and placed external to the cabinet to aid maintenance and minimize heat
build-up within the cabinet. The life rating is 50,000 hours for LED based on three-hour burning cycles.

To replace a lamp, it is necessary to remove the lamp assembly.

1. Switch cabinet light switch off.

2. Remove the screws at each upper side of the control center and allow the control center to rotate
down, resting on the safety straps.

3. The lamp is now directly exposed for replacement.

4. The lamp is removed by displacing the bulb to one side against the compressible bulb holder and lifting
out the lamp.

5. Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps, cable
or tubing during closure of the control center.

¹

Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributor

OM0329 Page 29 of 57
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7.3 HEPA Filter/Motor Replacement (Drawing BCD-16299)

CAUTION

!

The HEPA Filters under normal usage and barring an accident (a puncture), do not need replacement until the
efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM (.51 m/s)
(min.). This may permit the average downflow velocity to be as low as 55 LFPM (.28 m/s) as long as no point
falls below 20% or +/- 16fpm of the average downflow velocity, whichever is greater.

The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to be
biologically "clean".

7.3.1 Procedure (see Drawing BCD-16299)

Disconnect electrical power from the unit before attempting any maintenance action.

Step 1: Remove screws at each upper side of the control center and allow the control center to rotate

down, resting on the safety straps. Second, remove the front decorative panel which is held into
position by (3) knurled nuts on the top edge and (6) knurled screws on the front.

Step 2: Place sliding window into lowest position and remove front filter panel, which is held into position

by Phillip pan head screws. Once the screws are removed, the panel is held into position by
smooth weldstuds located on the top corner of the front filter panel. Use the window stop
brackets as handles to remove the panel.

CAUTION: Screws are used in lieu of acorn nuts, and lock washers.
The screws have O-rings and should be replaced if damaged or badly deformed.

The interior of the cabinet is now fully exposed for replacement of the filters and/or motor/blower.

Step 3: Filter Removal

It is not always necessary to replace both the supply and exhaust filters at the same time. If
during the course of certifications, the downflow falls off while the exhaust increases (i.e. greater
than 110 LFPM) (.56 m/s), the supply filter is "loading" faster than the exhaust filter, and only the
supply filter may need replacement. The opposite might also happen depending upon many
factors.

a. To remove the supply filter:

1. Remove the plenum screws in front of the supply HEPA filter.

2. Lift the permanent plenum and hold up with wire strap.

3. Carefully remove the supply filter. Direct exposure should be avoided.

Dispose of spent HEPA filters properly.
Avoid direct contact to "dirty side" of the filters.
Label toxic waste.

b. To remove the Exhaust HEPA:

1. Relax the exhaust filter seal loading mechanism by turning the four threaded bolts
counterclockwise until one can see a definite release of the loading springs.

2. Pull the exhaust choke tray free and remove the filter. It is not necessary to remove
the tray, although it is free to move forward several inches, if necessary, to free the
HEPA filter.

OM0329 Page 30 of 57
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Step 4: Filter Installation

Description:

Supply HEPA Filter

Exhaust HEPA Filter

Efficiency:

99.99% @ 0.3 Micron

99.99% @ 0.3 Micron

Airflow Rating:

100 fpm @ .55 ± .05" w.g. per sq. ft.

250 fpm @ .45 ± .05" w.g. per sq. ft.

Frame Type:

Metal

Metal

NU-677E-400

NuAire Part Number:

A-980979-01

A-980957-12

Filter Size:

21" (533mm) x 44" (1118mm) x 3" (76mm)

20" (508mm) x 22” (508mm) x 11 1/2" (292mm)

Filter Manufacturer:

Camfil Farr

Camfil Farr

NU-677E-500

NuAire Part Number:

A-980979-02

A-980957-13

Filter Size:

21" (533mm) x 56" (1422mm) x 3" (76mm)

20" (508mm) x 30" (660mm) x 11 1/2" (292mm)

Filter Manufacturer:

Camfil Farr

Camfil Farr

NU-677E-600

NuAire Part Number:

A-980979-03

A-980957-14

Filter Size:

21" (533mm) x 68" (1727mm) x 3" (76mm)

20" (508mm) x 36" (813mm) x 11 1/2" (292mm)

Filter Manufacturer:

A-980957-06

A-980957-09





When installing new filters, USE ONLY NUAIRE SPECFIED FILTERS FOR REPLACEMENT.

a. To install the supply filter, simply reverse the procedure outlines in Step 3a, above.

Note: Be sure to open the choke plate fully before inserting the filter into the tray.
This will assist in adjusting the airflow.

b. To install the exhaust filter, apply a thin layer of silicone grease to the top and bottom

gaskets of the filter and carefully insert into the exhaust choke tray.

Position the filter frame within the outside walls of the exhaust opening on the top of the hood.
Tighten the spring loaded bolts, 4 places, depressing the gasket material by 1/8 inch (3mm).

Step 5: Motor/Blower Assembly Removal

Note: Removal and replacement of the motor/blower may best be performed with the supply HEPA

filter removed to reduce the chance of damage to the fragile HEPA filter media.
Perform step 3.a. and place the supply in a secure location where it will not get damaged.

a. It is recommended that the motor/blower to be removed as a single unit.
To remove, disconnect electrical connections to the motor, remove the HEPEX pressure

plenum and unbolt the motor/blower assembly from the roof of the cabinet (4 places).
Always inspect the rubber isolation motor mounts and replace those that are cracked or
visibly show stress.

b. Replace the motor exactly as originally installed in the blower housing, paying particular

attention to the correct electrical connections (see Electrical Schematic).

c. Re-install the new motor/blower assembly.

OM0329 Page 31 of 57
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7.4 Sliding Window Replacement & Adjustment

The sliding window replacement is accomplished by removing the front decorative panel, control center, and
window glide assemblies. The sliding window adjustment may be required due to everyday use over the life of
the cabinet. The left window glide is stationary since it contains the micro switches that monitor window height.
Window adjustment is accomplished following instructions (see Drawing BCD-16376). When adjusting the
sliding window, be sure to verify proper micro switch operation. If the sliding window is too loose, the sliding
window will not properly activate the micro switches, thus causing potential operational malfunctions to occur.

7.5 Airflow Control System Setup and Calibration

7.5.1 General

The operation of the NU-677E cabinet requires that the setup and calibration procedures be performed
in order to certify or commission the cabinet for usage. The setup and calibration procedures
performed ONLY BY THE CABINET CERTIFIER ensure that cabinet's setpoints are verified and that the
airflow monitor sensor is calibrated to the correct values.

7.5.2 Configuration Parameters

Configuration parameters identify cabinet motor type and size for proper performance characteristics.

• Select/Verify cabinet model and size (Bold items represent default parameters)

LE

D indicates above both blower (red) and UV light keys will blink fast.

Review cabinet model size and change if desired as described below

o Press ↑ or↓ keys to scroll through the LED segment choices associated with

model/size.

Class II, 3 foot

Class II, 4 foot

Class II, 5 foot

Class II, 6 foot

Laminar, 3/4 foot

Laminar, 5/6 foot

o Press hidden key to accept model/size and exit

OM0329 Page 32 of 57
November/2021

Select motor control function/type

CAUTION

!

LE

D indicators above both blower (red) and light keys will blink fast.

Review motor control function/type and change it desired as described below.

o Press ↑ or ↓ keys to scroll through the LED segment choices associated with motor

control function/type.

Auto DC ECM motor

Fixed duty DC ECM motor

Fixed duty EC Impeller motor

o Press hidden key to accept motor control function/type and exit

7

.5.3 Airflow Calibrations
Failure to calibrate airflow to the specified requirements may
result in unsafe conditions of performance (i.e. product and/or
personnel protection, noise and vibration)

The NU-677E airflow calibration consists of adjustments to balance the airflow within the cabinet. THIS
WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN MEASURE THE AIRFLOW
FROM THE FILTERS WITH A SUITABLE VELOMETER. NuAire provides two adjustments to balance the

airflow within the cabinet.
These are:
a. PWM signal adjust via calibration parameter menu
b. exhaust filter choke

The PWM signal or blower speed adjustment establishes the motor speed controls curve starting point
of the programmed internal reference performance curve while the choke adjusts or balances the
exhaust airflow as well as makes up for filter resistance tolerances. Since it has been NuAire's
experience that the filters may not "load" evenly, choke adjustments may be necessary for proper
cabinet airflow balance and performance.

DC ECM motor PWM signal DC voltage should also be monitored and recorded upon final calibration.
The DC voltage may be measured using a digital voltmeter. The two test points to measure DC ECM
motor voltage are located on the DC motor connector on the main control board.

OM0329 Page 33 of 57
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The cabinet is considered to be certifiable if the following airflow measurements are present:
a. Downflow average: 60 LFPM ± 5 LFPM (.30 m/s ± .025 m/s).

b. Inflow average: 105 LFPM ± 5 LFPM (.53 m/s ± .025 m/s) using the direct inflow measurement

method or constricted 3 inch (76mm) high access opening measurement method.

Note: It is recommended to perform the airflow calibration process at least annually to optimize both

the airflow control and PresurFlow™ monitor systems.

OM0329 Page 34 of 57
November/2021

7.5.3.1 Downflow Calibration





Step 1: Place a velometer in the cabinet workzone on the horizontal plane 4 inches (102mm)

above the bottom edge of the viewing window. Spot check several points on the recommended
downflow velocity test grid in table 7.0

Step 2: If necessary, enter active blower speed adjustment.

ED indicator above blower (green) key will blink fast.

L

Step 3: Press ↑ or ↓ keys to adjust blower speed.

• LED segments will indicate blower speed percentage and active blower speed
adjustment

o Right end red LED indicates active blower speed adjust

The red LED will blink as soon as any adjustments are made and will continue to
blink as the motor rpm settles. Once the red LED stops blinking, the motor will run
steady state at the new percentage.

Note: The red LED must be non-blinking to save or exit

o Green LED’s indicate percentage on of scale (0-100%)
o Yellow LED’s indicate minimum (left/maximum (right) blower speed has been

achieved

Note: At any time during the process

• Press hidden key to accept and enter the blower speed calibration point (confirmed with 3
audible beeps).
(If the blower speed calibration point was not successfully entered with the 3 audible
beeps. The calibration process must then be repeated for successful entry of blower speed
calibration point.)

Step 4: Proceed to inflow calibration leaving in active blower speed adjustment

7.5.3.2 Inflow Calibration
Step 1: Measure the inflow velocity using the recommended procedure found in Table 7.0. If

necessary adjust the exhaust filter choke, located under the front decorative panel, to achieve the
correct average inflow velocity within the stated range of 105 ± 5 LFPM (.53 ± .025 m/s).

• Less than 100 LFPM (.51 m/s);
First open the choke plate or make sure it is open.
If this is insufficient, then increase blower speed.

• Greater than 110LFPM (.56 m/s)

First decrease blower speed to achieve ½ the exhaust excess, then close the choke
plate to achieve the balance. In this fashion, the downflow should remain nearly
constant (i.e. what the reduced speed took away, the choke plate restores).

Note: The choke plate adjustment requires a standard blade screwdriver.

To adjust, loosen the liquid-tight fitting around the choke adjustment shaft.
While monitoring the exhaust flow to check position, turning the choke adjustment shaft
clockwise will open the choke while turning counter clockwise closed the choke.

OM0329 Page 35 of 57
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Step 2: Once exhaust adjustment is complete, return to the downflow calibration process and





measure average downflow velocity. If the downflow average remains within the correct range, the
calibration is complete. Press hidden key to accept the blower speed calibration point. If not,
readjust as necessary to obtain the correct calibration range. Once entire cabinet has been
balanced, tighten liquid-tight fastener around choke adjustment shaft.

Note: Upon exit from the active blower speed adjustment, the PresurFlow™ will be calibrated
to the center green LED indicator. High/low alarm limits will be based on the default values
established from the calibration point.

7.5.3.3 PresurFlow™ Alarm Set Points

The PresurFlow™ alarm setpoints are based on the calibration setpoint. Once the calibration
setpoint is entered, based on a nominal inflow velocity of 105fpm (.53mls) the associated pressure
sensor value is entered as the nominal pressure value. High and low alarm setpoints are factory
verified and set if needed at 125fpm (.63mls) and 85fpm (.43mls).

However, if specific use alarm setpoints or re-entry of recommended alarm setpoints is desired during the calibration process, the alarm setpoints may be adjusted by performing the following:

Low Alarm Setpoint

• Press and hold the Hidden and ↓ key for 3 seconds. (The left red LED will blink and the green LED’s indicate blower speed)

• Press ↑ or ↓ keys to adjust blower speed to the desired airflow velocity low alarm setpoint

value.

• Left end red LED indicates active low limit blower speed adjust
The red LED will blink as soon as any adjustments are made and will continue to blink as the
motor rpm settles. Once the red LED stops blinking, the motor will run steady state ate the
new percentage.
If the low alarm setpoint value is not within an acceptable range, the left end red LED will
blink at a very fast rate.

Note: The red LED must be non-blinking to save or exit.

Note: At any time during the process

• Press hidden key to accept low alarm setpoint value

• Press and hold outlet key for three seconds to remove any previous offsets

• Up

OM0329 Page 36 of 57
November/2021

on exiting, the blower will go back to actual airflows.





High Alarm Setpoint

• Press and hold the Hidden and ↑ key for 3 seconds. (The right red LED will blink and the green LED’s indicate blower speed)

• Press ↑ or ↓ keys to adjust blower speed to the desired airflow velocity high alarm setpoint

value.

• Red end Red LED indicates active high limit blower speed adjust.
The red LED will blink as soon as any adjustments are made and will continue to blink as the
motor rpm settles. Once the red LED stops blinking, the motor will run steady state at the
new percentage.
If the high alarm setpoint value is not within an acceptable range, the right end red LED will
blink at a very fast rate.

Note: The red LED must be non-blinking to save or exit.

Note: At any time during the process

• Press hidden key to accept high alarm setpoint value

• Press and hold outlet key for three seconds to remove any previous offsets

• Up

on exiting, the blower will go back to actual airflows.

Note: Specific use alarm setpoints or the offset pressure value from the nominal calibration

point will be maintained with a new nominal calibration value. It is not necessary to
re-enter the alarm setpoints after a nominal calibration.

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7.5.3.4 PresurFlow Alarm Verification



The PresurFlow Alarm setpoints are based on the calibration setpoint. Once the calibration setpoint

is entered, the Alarm setpoint offset pressure values will align from the calibration pressure value.
The high or low alarm setpoint can be verified by measuring inflow volume/velocity while adjusting
blower up or down within the Alarm Verification menu.

• Note:

If blower was off while entering into the Alarm Verification Menu, the low alarm limit
will immediately activate. Turn on blower; once airflow is above the low alarm limit, the
alarm will turn off.

• LED segments will indicate blower speed percentage.

• Press ↑ or ↓ key to raise or lower blower speed. Alarm is active so yellow and red LED’s

will activate if pressure reaches the low or high alarm limit.

7.5

.3.5 Nite Care Calibration (See Section 8.2.2 to Activate the Nite Care Function)

The Nite Care mode is defaulted to operate the blower at approximately 600 rpm or a 14% duty cycle. However, if desired the Nite Care blower speed can be adjusted higher or lower by performing the following:

indicator above blower (green) key will blink fast.

LED
Press ↑ or ↓ keys to adjust blower speed.

• LED segments will indicate Nite Care blower speed percentage and active blower speed
adjustment

o Left end red LED indicates active blower speed adjust

The red LED will blink as soon as any adjustments are made and will continue to blink as the
motor rpm settles. Once the red LED stops blinking, the motor will run steady state at the
new percentage.
Note: The red LED must be non-blinking to save or exit

o Green LED’s indicate percentage on of scale (0-100%)
o Yellow LED’s indicate minimum (left/maximum (right) blower speed has been achieved

Note: At any time during the process

• Press hidden key to accept Nite Care blower speed calibration point

OM0329 Page 38 of 57
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Table 7.0

Model Size

Window Access Height

7.0

(178)

12.396
(315)

17.792
(452)

23.188
(589)

28.584
(726)

33.980
(863)

39.375

(1000)

7.25

(184)

13.518
(343)

19.786
(503)

26.054
(662)

32.322
(821)

38.590
(980)

44.858

(1139)

51.125

(1229)

7.5

(191)

15.410
(391)

23.320
(592)

31.230
(793)

39.140
(994)

47.050

(1195)

54.960

(1396)

62.870

(1597)

7.0

(178)

7.250
(184)

7.5

(191)

12.5

(318)

12.5

(318)

12.5

(318)

18.0

(457)

17.750
(451)

17.5

(445)

A. Downflow Measurement

a. Recommended Instruments: TSI 8355 Thermo anemometer
b. Procedure: Supply filter efflux is measured on a grid, in a horizontal plane 4 inches (102mm) above the bottom edge
of the window. No reading should be taken closer than 6 inches (152mm) from the inside perimeter.
c. Test Data - Inches (mm):

Recommended Measurement Methods for Cabinet Downflow and Inflow

400E

500E

600E

Number of Readings: Average Velocity ft./min.(m/s)

d. Acceptance Criteria:

1. Average downflow velocity = 55 to 65 fpm (.28 to .33 m/s)

2. Individual readings must be within + 20% or + 16fpm (+ 0.08m/s) whichever is greater (factory test) or
+ 25% or + 16fpm (+ 0.08m/s) whichever is greater (field test) from the average downflow velocity.

B. Inflow Measurement

a. Recommended Instrument: Shortridge Flowhood ADM-870 or TSI 8355 Thermo anemometer.
b. Primary Procedure:

The primary procedure to determine inflow velocity uses a Direct Inflow Measurement (DIM) Instrument (i.e.
Shortridge flowhood). The DIM Instrument can be used directly on the cabinet with NO CORRECTION FACTORS
REQUIRED if operated in the local density default mode. NSF has tested the cabinet and established listed air
velocities. Use duct tape to secure the DIM Instrument to the cabinet preventing any sneak air paths from occurring.

The DIM Instrument will read inflow volume (i.e. CFM). Use the window access opening area to calculate inflow
velocity.

OM0329 Page 39 of 57
November/2021

Inflow Volume
ft.3/min.(m3/s)

Access Opening
ft.2(m2)

Inflow Velocity
ft./min(m/s)

4

8.264

12.528

16.792

21.056

25.320

29.584

33.848

38.112

42.375

4

8.198

12.396

16.594

20.792

24.990

29.188

33.386

37.584

41.782

45.980

50.178

54.375

Average Velocity of Constricted Area
ft./min.(ms)

3" (76mm)

Constricted

12" (305mm)

12"(305mm)

Window

.97

(.090)

3.86

(.358)

1.22

(.113)

4.86

(.451)

1.47

(.137)

5.86

(.544)

300E

Alternate Procedure:
The alternative procedure to determine inflow velocity uses a thermo anemometer in a constricted window access
opening of 3 inches (76mm) with the armrest removed. Inflow air velocity is measured in the center of the constricted
opening 1-1/2 inches (38mm) above the work access opening on the following specified grid. Use the correction factor
table to calculate the inflow velocity.

c. Test Data - Inches (mm):

1. DIM Measurement

2. Constricted 3 inch (76mm) high access opening measurement - Inches (mm):

4

8.3966

(102)

(213)

12.792
(325)

17.188
(437)

21.584
(548)

25.980
(660)

30.375
(771)

400E

500E

600E

(102)

(102)

4

(102)

(210)

(208)

8.158
(207)

(318)

(315)

12.316
(313)

(426)

(421)

16.474
(481)

(535)

(528)

20.632
(524)

(643)

(635)

24.790
(630)

(751)

(741)

28.948
(735)

(860)

(848)

33.106
(841)

(968)

(955)

37.264
(946)

(1076)

(1061)

41.422
(1052)

(1168)

45.580
(1158)

(1274)

49.738
(1263)

(1381)

53.896
(1369)

58.054
(1475)

62.212
(1580)

Number of Readings:

1. Average Velocity of Constricted Area fpm (m/s)

2. X Constricted Access Area ft2 (m2)

3. = Constricted Area Volume CFM(m3/s)

4. Constricted Area Volume CFM(m3/s)

5.

12" (305mm) Access Window Area ft2(m2)

÷

6. = Average Velocity of 12" (305mm) Access Window Area fpm (m/s)

7. Average Velocity of 12" (305mm) Access Window Area fpm (m/s)

8. X Correction Factor for Window Height

66.375
(1686)

9. = Average Inflow Velocity fpm (m/s)

d. Acceptance Criteria: Access Opening Inflow Velocity = 100 to 110 fpm (.51 to .56 m/s)

Cabinet Size

400E

500E

600E

OM0329 Page 40 of 57
November/2021

Areas/Correction Factors for Calculations

Window Access Opening Correction Factor For

1.03

1.03

1.0

7.6 HEPA Filter Leak Test

Model Size

*Supply Area

(ft2)(m2)

Exhaust Area

(ft2)(m2)

Model Size

*Supply Area

(ft2)(m2)

Exhaust Area

(ft2)(m2)

500E

10.74 (.998)

4.17 (.387)

400E

8.53 (.792)

3.06 (.284)

600E

12.95 (1.203)

5.00 (.465)

=

=



In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly accessible by the
measuring instrument. The challenge material (i.e. PAO) should be supplied in the rear center of the workzone
over the intake slots. The upstream challenge port being common for both filters is located under the work
surface with a red cap.

7.6.1 Supply Filter

The diffuser plate, placed below the HEPA to protect the filter during normal usage, may be removed as
follows:
The diffuser is secured to the cabinet shell by #1/4-20 acorn nuts located immediately behind the front
viewing window. After removing the fasteners, drop the front of the diffuser plate several inches and pull
forward gently.
Note that the diffuser is purposely a tight fit - it is held to the back wall of the cabinet interior by a light
push - fit with projecting studs. To avoid the window high alarm during the filter integrity check, it is
desirable to enter the Service menu and turn on the blower.

Air currents in the room can sometimes contribute to exhaust entrainment on top of the cabinet. This can
lead to false leak readings. In order to reduce the probability of false leak readings, it is recommended to
cover half of the exhaust HEPA filter while scanning the uncovered section of the exhaust HEPA filter.

7.6.2 Exhaust Filter

The exhaust filter is typically more difficult to check since protective grills, charcoal filters, or exhaust
transitions could cover the filter. Access panels are usually provided and should be removed. If an air gap
exhaust transition is provided, the air gap must be sealed with duct tape or other suitable means to
prevent contaminated air from migrating into the exhaust efflux. All exhaust blowers/fans should be
turned off during the check.

NOTE: To avoid the window high alarm during the filter integrity check, it is desirable to enter

into the Blower speed adjustment menu and turn the blower on. To accomplish this, perform
the following:

LE

D segments will indicate blower speed percentage. However no adjustment is needed, do not adjust!

• Once filter integrity test is completed,

• Press key to abort and exit.

Note: If the upstream challenge port is deemed contaminated and not accessible, use both

downflow and exhaust volume for determining challenge concentrations. Use following
area information below with average downflow velocity and spot-check exhaust velocities
as measured to determine volume (CFM) (CMH).

* Measured 4 inches above the bottom edge of the window.

Laskin Nozzle Concentration Formula

# Nozzles x 135 CFM x 100 ug/L Challenge
Downflow (CFM) + Exhaust (CFM) Concentration (ug/L)

# Nozzles x 229 CMH x 100 ug/L Challenge
Downflow (CMH) + Exhaust (CMH) Concentration (ug/L)

OM0329 Page 41 of 57
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7.7 Airflow Smoke Pattern Test



The airflow smoke pattern test is performed using a smoke source (i.e. smoke tubes) in and around the cabinet workzone and access opening to determine a visual representation of the cabinet’s containment performance. To perform the test, the smoke source should be passed through the following areas: A smoke source shall be passed:

1. From one end of the cabinet to the other, along the center line of the work surface, at a height of 4 inches (102mm) above the top of the access opening.

2. From one end of the cabinet to the other, 1 inch (25mm) just inside the view screen, at a height 6 inches (152mm) above the top of the access opening.

3. Along the edges of the entire perimeter of the work opening approximately 1.5 inches (38mm) outside the cabinet, with particular attention paid to corners and vertical edges.

4. 2 inches (51mm) from the sides up inside of the window at the side channel seals, and along inside of the cabinet along the top of the work area or immediately below the wiper gasket.

The criteria used to evaluate the smoke patterns is the following:

1. The smoke inside the cabinet shall show smooth downward flow with no dead spots or reflux.

2. No smoke shall escape from inside the cabinet.

3. No smoke refluxes out of the cabinet once drawn in, nor does smoke billow over the worksurface or penetrate onto it.

4. No smoke shall escape from the cabinet.

Note: If using BSC in more than one location, follow the NSF/ANSI 49 guidelines below.

* NSF/ANSI 49 Annex E.3.3.6 Mobile Installations

Mobile Base stands with and without lift capability have been used when the BSC is operated in multiple locations
in the same or adjoining laboratories. Proper cabinet operation should be confirmed by airflow smoke pattern tests at
each site of use. If the cabinet is relocated to another facility, or subjected to excessive shock and/or vibration during
moving, the BSC should be recertified to ensure it is functioning in a proper manner

7.8 Site Installation Assessment Tests

These tests are performed to verify the sash position, airflow or pressure setpoint where an audible and/or
visual alarm will activate to signify unfavorable operating conditions within the Biosafety cabinet and/or the
remote exhaust blower, and canopy connection performance.

7.8.1 Sash Alarm Step 1: With sash alarm switch enabled, raise the sliding sash 1” (2.5cm) above the manufacturer’s

designated sash height for normal operation. Verify that the audible/visual alarm
activates/sounds.

7.8.2 Airflow or Pressure Alarm (when installed)

7.8.3 Exhaust System Performance

OM0329 Page 42 of 57
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Step 2: Return the sash to its normal operating height.

Step 1: Measure and record the speed control operating voltage at the speed control test points

using a voltmeter.

Step 2: Using the primary or secondary inflow test method, lower the speed control voltage to

reduce the inflow by 20% from the certified testing value.
Step 3: Verify that the alarm activates when the inflow is dropped to this point.
Step 4: Adjust alarm setpoint as necessary as instructed by the alarm manufacturer procedures.
Step 5: Return the speed control to its certified operating voltage as measured by the voltmeter.

Canopy Exhaust Transitions

Step 1: Introduce a visible medium source into the canopy air intake(s) while slowly reducing the

exhaust volume until there is a loss of capture of the visible medium into the canopy air

intake(s).
The audible and visual canopy alarms shall respond within 15 seconds, and the cabinet

fan(s) will continue to operate.
Step 2: Return exhaust volume to original value.

CAUTION

!

CAUTION

!

NOTE: Positive pressure ductwork conditions are strongly discouraged. Positive pressure ductwork
conditions should be reviewed and evaluated by a cognizant Safety Officer of Industrial Hygienist as part of their
Laboratory Ventilation Management Program and Chemical Hygiene Plan.

7.9 Main Control Board Description and Replacement

To access the main control board for fuse or board replacement, remove screws at each upper side of the
control center and allow the control center to rotate down, resting on the safety straps. Now the main control
board is exposed for service.

7.9.1 Main Control Board Replacement

The main control board consists of one Printed Circuit Board (PCB) assembly.
The PCB contains the power supply, configuration switch, sensor inputs/outputs and control
inputs/outputs components and display.

7.9.2 Main Control Board Fuse Replacement

Disconnect electrical power from cabinet before fuse replacement.

All AC circuits are fuse protected and when replacement is necessary, USE ONLY FUSES OF SAME TYPE
AND RATING FOR PROTECTION AGAINST RISK OF FIRE.

DESCRIPTION: BLOWER FUSE OUTLET FUSE ACCESSORY OUTPUT FUSE LIGHT FUSES

FUSE TYPE: TIME-LAG TIME-LAG TIME-LAG TIME-LAG

FUSE SIZE: 1/4 X 1-1/4 INCH 5 X 20MM 5 X 20MM 5 X 20MM

NU-677E-400 8 AMPS 3 AMPS 2 AMPS 1 AMP (2)

NU-677E-500 10 AMPS 3 AMPS 2 AMPS 1 AMP (2)

NU-677E-600 10 AMPS 3 AMPS 2 AMPS 1 AMP (2)

7.9.3 Main Control Board Replacement

Note: All setup and calibration data will be lost, the memory reinitialized to the default values and all

control functions reset to an initial cabinet power condition.

If possible, before the main control board replacement, it would be preferred to know the

operational parameters of the cabinet, (i.e. blower speed/PWM signal DC voltage setpoints if
modified and airflow data from previous certification).

The main control board is fastened to the control center with (6) 6-32 studs/nuts. All electrical
connections are made with removable terminals and/or Faston connectors except for the motor/blower
connector which uses a screw terminal. Remove all electrical connections and fasteners and then
remove the main control board from the control center.

Install new main control board by reattaching all electrical connections and fasteners. Once installed,
rotate control center to normal position and fasten in place.

Reconnect power to cabinet.

OM0329 Page 43 of 57
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7.9.4 Aeromax™ Control System Reset
The Aeromax™ control board has a reset function available for Qualified Service Personnel.

Master Reset-Resets all calibration, cabinet size, motor type/function and option settings back to their

default condition. It is recommended to perform a master reset upon installation of replacement
control board or if there seems to be intermittent functional abnormalities.

Perform the following sequence to accomplish a master reset:

1. Turn off all functions i.e. blower, lights, etc.

2. Press and hold alarm silence key for 10 seconds until alarm LED flashes, then release.

3. Press hidden key within 5 seconds of the alarm LED flashing. There will be a (3) beep

confirmation that the reset has occurred.

4. Turn off cabinet (either unplug cabinet or use power switch on left side within control panel)

then turn back on to reinitialize the Aeromax™ control board. Just as in the initial power up, the
RED alarm LED will blink to indicate power up status.

6. E

nter into service parameters and input configuration for calibration and option settings as

required.

8.0 Error Messages, Troubleshooting, Option-Diagnostics and Airflow Sensor Performance
Verification

Audible alarms and error messages occur for a variety of reasons. Whenever an alarm condition has been
present for a period of at least 10 seconds, the audible alarm/error message will be presented and stay on until
the error is cleared. The audible alarm will be on for 30 seconds upon initial alarm condition, then once every
ten seconds. When presented with an error message, please perform the following:

Step 1: NOTE ALL ERROR MESSAGES.
Error message will appear on the control panel with red LED’s.
Step 2: VERIFY ERROR MESSAGES.

Error messages can be verified by clearing the error function by either turning the blower or the

cabinet on and off.
Step 3: MONITOR RE-OCCURRENCE OF ERROR MESSAGES.
If re-occurrence of the error message is immediate or daily, use the following guide to correct the

situation.

OM0329 Page 44 of 57
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8.1 Error Message Troubleshooting Guide

Error Message

Error Description

Correction

Window Alarm
(Window High)

Sliding window is above standard working height
or micro switch is not operating properly.

Verify standard working height and window micro
switch operation.

Window Alarm

Window Closed

Sliding window is below standard working height

Indicates the window is in the full down position

Check light fuse on main control board.

Check light starters, if present. Check ballast.

Check blower fuse on main control board.

Check DC motor PWM signal on main control board.

Red alarm LED blinks

Indicates a power interruption has occurred.

Press any key to clear.

Cabinet outlets won't
turn on.

Blue LED above outlet key indicates the outlets
should be on.

Check outlet fuse located on main control board.
Check voltage coming out of main control board.

Check sliding window position- should be fully

Check ballast.

Check for short on output of fuse.

etc. to isolate the short.

UV LED blinks fast and
red LED alarm

Blower green LED

LED’s indicators on

PresurFlow™ left red

red LED alarm

Check airflow values.

Recalibrate PresurFlow™ system.

PresurFlow™ right red

red LED alarm

All PresurFlow™ LED’s
blink

Message acknowledges new firmware was
loaded into microprocessor

Check connectors and wires from main control

Replace motor if required

(Window Low)

Cabinet LED lights
won’t Turn on

Cabinet blower won't
turn on.

Cabinet ultraviolet
light
won't turn on.

Blower or light fuse
continues to blow
after replacement.

or micro switch is not operating properly.

Blue LED above light key indicates the lamp
should be on.

Green LED above blower key indicates the
blower should be on.
Airflow Alarm.

Yellow LED above UV light key indicates the UV
lamp should be on.

N/A

Verify standard working height and window micro
switch operation.

Check LED lamps.
Check voltage coming out of main control board to
light ballasts.

Check AC voltage coming out of main control board.
Check wiring to blower.
Check blower motor.

closed.
Check blower/lights fuse on main control board.
Check voltage coming out of the main control board
to ultraviolet light ballast.

Isolate output of fuse by disconnecting control
center
connectors, light circuit, AC or DC blower circuit,

Indicates that the UV light needs replacement Replace UV light and clear UV run time clock.

blinks fast and
PresurFlow™ 3 green

LED indicator on and

LED indicator on and

Blower red LED blinks
and red LED alarm

Indicates that the Nite Care is activated,
preventing the usage of the cabinet.

PresurFlow™ reading low flow (pressure)

PresurFlow™ reading high flow (pressure)

Indicates that the motor rpm signal has been
interrupted

OM0329 Page 45 of 57
November/2021

N/A

Check blower function.

Check airflow values.
Recalibrate PresurFlow™ system.

N/A

board
to the motor

8.2 Option Parameters

The option parameter menu allows A QUALIFIED TECHNICIAN to configure several different optional
parameters per the menu as described below.

8.2.1 Sync Function with Active Blower
To access the option parameter menu, perform the following:

d LED indicator above the blower key will blink fast

Re

The PresurFlow™ blinking green LED segments will indicate seven optional parameters as shown and

described below. The UV Light key (move lefts) and outlet key (move right) allows selection of the option
parameter desired.

Once the desired option parameter is indicated, press ↑ or ↓ key to turn on or off. A slow blinking green

LED indicator means off and a fast blinking green LED indicator means on. Multiple option changes can be
selected.

• Pressing the hidden key will accept all changes and exit

Sync Fan Relay with Active Blower -
Normally the fan relay will activate when the blower switch is pressed. Blower can
either be actively running or pending. If the fan relay sync is active the blower must
be actively running for the relay to change state.

Sync Accessary Outlet with Active Blower –
Normally the accessary outlet is on all the time. If the accessary outlet sync is active,

the blower must be actively running for the accessary outlet to turn on.

Sync Outlet Power with Active Blower –

Normally the outlet power is turned on via the outlet key. If the outlet power sync is
active, the outlet power will turn on and off with the blower or may be turned on and
off independently if the blower is active.

Sync LED Light with Active Blower –

Normally the LED light is turned on via the LED light key. If the LED light sync is
active, the LED light will turn on and off with the blower or may be turned on and off
independently if the blower is active.

Sync 15 Volt DC output with active blower normally the 15 Volt DC output located on

the control board is on when power is applied to the system. If the 15 Volt DC
output sync is active, the blower must be actively running for the 15 Volt DC output
to turn on.

8.2.2 Blower/Airflow Options

To access the option parameter menu, perform the following:

Red LED indicator above the blower key will blink fast

The PresurFlow™ blinking green LED segments will indicate seven optional parameters as shown and
described below.
The UV Light key (moves left) and outlet key (moves right) allows selection of the option parameter
desired.

Once the desired option parameter is indicated, press ↑ or ↓ key to turn on or off. A slow blinking green
LED indicator means off and a fast blinking green LED indicator means on. Multiple option changes can be
selected.

• Pressing the hidden key will accept all changes and exit

OM0329 Page 46 of 57
November/2021



Require Password –
Normally it is not required to use a password (i.e. 3 key press sequence of the blower
and hidden key). If the option is turned on, it would be required to use the correct
password to turn on the blower. The default password once turned on is blower­hidden-blower keys in sequence. The password can be changed in the blower
password option menu.

Allow UV light anytime –

Normally the UV light is interlocked with the window being in the closed position.
For service purposes only, if the function is active, the UV light may be turned on at
any window height.

Note: In addition to the Allow UV light anytime system function, there is a double redundant UV

light window interlock relay. To override the UV light window interlock relay, the relay
itself must also be shorted. (See electrical schematic for reference).

Manual Blower Restart –

Normally when the blower is actively running and a power interruption occurs.

The blower will automatically come back on when power is restored. If this function
is turned off, the blower will not automatically come back after a power interruption,
but would require the user to press the blower key to restart the blower.

Nite Care –

Normally the Nite Care function is turned off. If selected and turned on, once the
blower is actively running. Upon closure, the blower will continue to run at a
calibrated lower speed level to keep the workzone interior sterile. The PresurFlow™
will indicate(s) blinking green LEDs along with green LED above blower key. The LED
light will turn off (blue LED above light key will blink if window closure turned it off)
and become inoperable; however the UV light can be used.

Disable PresurFlow™ -
If this function is active, the digital pressure sensor and alarm function are turned

off.

Temporary Nite Care –
When this option parameter is turned on and the other requirements below are
met, the blower key (when held for 5 seconds) will toggle blower between normal
and Nite Care blower speed. The Nite Care blower will time out, based on the Auto
Timer duration for Nite Care without a blower key press and the blower speed will
revert back to normal. Closing the window will automatically terminate.

In addition to turning this option on the following requirements must also be met

• The Nite Care must be selected

• Password must be selected

• Window is at normal height and blower is running

• Nite Care blower auto timer must be set for a minimum of 15 minutes.

Disable audible alarms / audible key feedback

Normally audible alarms from the PresurFlow™ monitor and audible key feedback
are present to provide an audible sound to the user. If this function is active, all
audible sound will be silenced when the control system is in normal run mode.
Audible sound will still occur in any service/calibration menu function.

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9.0 Remote Contacts

The NU-677E has several contact closures for remote sensing of various functions.

9.1 Fan Relay

The fan relay contacts are normally open and closed contact closure outputs that are activated whenever the
blower key is pressed and the blower key LED indicator is on or blinking. Contact ratings are 250 VAC maximum
at 2 Amps.

9.2 Alarm Relay

The alarm relay contacts are normally open and closed contact closure outputs which are activated whenever an
airflow alarm condition occurs. Contact ratings are 250 VAC maximum at 2 Amps.

9.3 15VDC Output

The 15VDC (100mA) output is generated if the blower is actively running.

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

10.0 Optional Equipment

CAUTION

!

10.1 Ultraviolet Light

10.1.1 Overview

The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms
in the air or on directly exposed surfaces. Approximately 95% of the ultraviolet radiations from
germicidal tubes are in the 253.7 manometer region. This is a region in the ultraviolet spectrum which is
near the peak of germicidal effectiveness. The exposure necessary to kill bacteria is the product of time
and intensity. High intensities for a short period of time, or low intensities for a longer period are
fundamentally equal in lethal dosage on bacteria (disregarding the life cycle of bacteria). The intensity
of light falling on a given area is governed by the inverse law; that is the killing intensity decreases as the
distance increases from the tube.

The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per
square centimeter (for a new tube) falling on a horizontal plane defined by the bottom of the work
surface. The minimum requirement per paragraph 5.12 of NSF Standard 49 is 40 microwatts per
square centimeter (ref. NSF Std. #49, June, 1976).

Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be
closed while the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.

10.1.2 Operation

The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV
switch location on the front panel. The sliding window is interlocked to the ultraviolet light, so when the
sliding window is raised, the ultraviolet light will turn off.

10.1.3 Precaution

The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening
of the skin, if of sufficiently high intensity, or if exposure covers a prolonged period of time. For this
reason, one should avoid direct eye and skin exposure to ultraviolet light. If exposure cannot be
avoided, it is necessary for personnel to wear eye goggles or face shields, and long sleeve gowns with
rubber gloves.

Ultraviolet light will injure your eyes.
Avoid direct viewing at all times.
Personnel should not be present when ultraviolet lamp is on.

Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be
closed while the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.

10.1.4 Maintenance

The output of an ultraviolet light deteriorates with burning age.
The useful life of the light is approximately 7000 hours under specific test conditions.

Note: Before testing with lamp off, the light may be cleaned with a lint-free cloth
dampened with alcohol or ammonia and water

It is recommended that either a time schedule be established or the tube's output be measured
periodically and the tube replaced when its output falls below 40 microwatts per square centimeter or
exceeds 7000 hours of operation. Lights should be allowed to operate approximately 5 to 10 minutes
(longer when the light is in low temperatures) to warm up sufficiently and wiped clean of dust or dirt
before reading the output with a meter. Even minute amounts of dust will absorb ultraviolet energy.
The light may be cleaned with a lint-free cloth dampened with alcohol or ammonia and water.

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Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations (e)

Microwatt

per cm/2

Microwatt

per cm/2

Penicillum roqueforti

26,400

Paramecium

200,000(a)

Penicillium expansum

22,000

Penicillium digitiatum

88,000

Nematode Eggs

40,000(b)

Aspergillus glaucus

88,000

Aspergillus flavus

99,000

Algae

22,000(c)

Aspergillus niger

330,000

Rhizopus nigricans

220,000

Virus

Mucor racemosus A

35,200

Baceriophage (E. Coli)

6,600

Mucor racemosus B

35,200

Tobacco Masaic

440,000

Oospora lactis

11,000

Influenze

3,400(d)

Yeasts

Saccharomyces

13,200

ellipsoideus

17,600

Saccharomyces cerevisiae

13,200

Brewers' yeast

6,600

Baker's yeast

8,800

Common yeast cake

13,200

Bacteria

Streptococcus lactis

8,800

Strep. hermolyticus (alpha type)

5,500

Staphylococcus aureus

6,600

Staphylococcus albus

5,720

Micrococcus sphaeroides

15,400

Sarcina lutea

26,400

Pseudomonas fluorescens

7,040

Escherichia coli

7,040

Proteus vulgaris

7,480

Serratia marcescens

6,160

Bacillus subtilis

11,000

Bacillus subtilis spores

22,000

Spirillum rubrum

6,160

Mold Spores

seconds

Protozoa

seconds

References:

(a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy, D. Van Nostrand o.,
New York, New York, pp 253
(b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162
(c) Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp., pp. 713-714
(d) Hollaender, A., Oliphant, J.W. (1944) The inactivation effect of monochromatic ultraviolet.
Radiation on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454
(e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations" comes from

Westinghouse brochure entitled - "Westinghouse Sterilamp Germicidal Ultraviolet Tubes"

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11.0 Electrical/Environmental Requirements

WARNING

!

11.1 Electrical (Supply voltage fluctuations not to exceed +/- 10%)

*NU-677-400E 115V 60Hz 1 Phase 15 Amps

*NU-677-500E 115V 60Hz 1 Phase 16 Amps
*NU-677-600E 115V 60Hz 1 Phase 16 Amps

*CE

11.2 Operational Performance (for indoor use only)

Environment Temperature Range: 60°F-85°F (15°C - 30°C)
Environment Humidity: Maximum relative humidity 80% for temperatures up to 31°C

decreasing linearly to 50% relative humidity at 40°C

Environment Altitude: 6562 Feet (2000 meters) Maximum

11.3 Light Exposure

Standard LED Lighting @ 150 ft. candles (1614 LUX) maximum intensity.

11.4 Installation Category: 2.0

Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is
designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection
means. For example, in CAT II, which is the category used for instruments in installations supplied from a supply
comparable to public mains such as hospital and research laboratories and most industrial laboratories, the
expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply.

11.5 Pollution Degree: 2.0

Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution
degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of
occasional conductivity caused by condensation.

11.6 Chemical Exposure

Chemical exposure should be limited to antibacterial surface disinfectants used for cleaning and disinfecting. USE OF
CHLORINATED OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL. Equipment decontamination

can be accomplished by non-condensing gas or vapor Paraformaldehyde, Hydrogen Peroxide or Chlorine Oxide following
NSF/ANSI 49, Annex G.

11.7 EMC Performance (classified for light industrial)

Emissions: EN61326
Immunity: EN61326

Class A equipment is intended for use in an industrial environment.
In the documentation for the user, a statement shall be included drawing
attention to the fact that there may be potential difficulties in ensuring
electromagnetic compatibility in other environments, due to conducted as
well as radiated disturbances.

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12.0 Disposal and Recycle

Component

Material

Base Cabinet

Stainless Steel

Front Grill

Stainless Steel

Worksurface

Stainless Steel

Window Faring

Stainless Steel

Window Glides

HDPE

Window

Safety Glass

Window Frame

Stainless Steel

Front Service Panel

Painted Steel

Front Decorative Panel

Painted Steel

Control Center

Painted Steel

Supply Diffuser

Aluminum

Exhaust Filter

Aluminum

HEPA Filter Frames

Painted Steel

Hepex Bag

PVC

Blower Wheel & Housing

Steel

Motor

Various Steel/Copper

Printed Wiring Assembly

Lead Free Electronic

Wire

PVC Coated Copper

Ballasts

Various Steel, Electronic

Armrest

Stainless Steel

Connectors

Nylon

Hardware

Stainless Steel and Steel

NOTE:



BIOHAZARD
RECYCLE



LEAD FREE

CAUTION

!

Cabinets that are no longer in use and are ready for disposal contain reusable materials. ALL components with
the exception of the HEPA filters may be disposed and/or recycled after they are known to be properly
disinfected.

Follow all local, state and federal guidelines for disposal of HEPA filter solid waste.

Prior to any disassembly for disposal the cabinet must be decontaminated

Note: Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic.

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