Page 1
OM0318 Page 1 of 63
LabGard® ES Energy Saver
Class II Laminar Flow
Biosafety Cabinet
Models
NU-540-300E/400E/500E/600E
Bench/Console
Operation and Maintenance Manual
October, 2021
Revision 1
NU-540-300E/400E/500E/600E
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
Page 2
OM0280 Page 2 of 63
Rev 1 October/2021
Congratulations!
You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care,
maintenance (certification), and laboratory procedure, this cabinet will give you years of product and
personnel protection from particulate contaminants as prescribed in EN 12469 and NSF/ANSI 49. Please read
this manual carefully to familiarize yourself with proper installation, maintenance and operation of the
cabinet. Other reference and guideline materials are available through the following web sites;
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.cabs-acsb.ca
www.ebsaweb.eu
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.nuaire.com
Page 3
OM0280 Page 3 of 63
Rev 1 October/2021
About this Operation and Maintenance Manual
The information contained in this manual is intended to reflect our current production model along
with the more frequently purchased options. Any unique additions, modifications, or shop drawings are
appended in the back flap of this manual, along with any modifications and/or additions to procedures as
outlined in this manual. A copy of the original factory test report is also appended to this manual. In case this
manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be
obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief description
of the information desired.
Page 4
OM0280 Page 4 of 63
Rev 1 October/2021
LabGard® ES Energy Saver
Class II Laminar Flow Biosafety Cabinet
Models NU-540-300E/400E/500E/600E
Operation and Maintenance Manual
Table of Contents
Section No. 1 ..................................................... General Information
Section No. 2 ..................................................... Models and Features
Section No. 3 ..................................................... Warranty
Section No. 4 ..................................................... Shipments
Section No. 5 ..................................................... Installation Instructions
5.1 ..................................................................... Location
5.2 ..................................................................... Set-up Instructions
5.3 ..................................................................... Testing Methods and Equipment
Section No. 6 ..................................................... Operating the NU-540E
6.1 ..................................................................... Aeromax™ Control System
6.2 ..................................................................... Operating Guidelines
6.3 ..................................................................... Operating Sequence
6.4 ..................................................................... Ergonomics
6.5 ..................................................................... Cleaning Procedures
Section No. 7 ..................................................... General Maintenance
7.1 ..................................................................... Decontamination
7.2 ..................................................................... LED Lamp Bulb Replacement
7.3 ..................................................................... HEPA Filter/Motor Replacement
7.4 ..................................................................... Sliding Window Replacement and Manual Adjustment
7.5 ..................................................................... Airflow Control System Setup and Calibration
7.6 ..................................................................... HEPA Filter Leak test
7.7 ..................................................................... Airflow Smoke Pattern Test
7.8 ..................................................................... Site Installation Assessment Test
7.9 ..................................................................... Cleanliness Classification Test for Pharmacy Application
7.10 ................................................................... Main Control Board Description and Replacement
Section No. 8 .................................................... Error Messages, Troubleshooting and Option-Diagnostics
Section No. 9 ..................................................... Remote Contacts
Section No. 10 ................................................... Optional Equipment
10.1 ................................................................... Ultraviolet Light
Section No. 11 ................................................... Electrical/Environmental Requirements
Section No. 12 ................................................... Disposal and Recycle
Manual Drawings
ACD-16512 ...................................... NU-540E Airflow Schematic
BCD-16887 ...................................... NU-540-300E Specification Drawing
BCD-16888 ...................................... NU-540-400E Specification Drawing
BCD-16889 ...................................... NU-540-500E Specification Drawing
BCD-16890 ...................................... NU-540-600E Specification Drawing
BCD-16882 ...................................... Drain Valve Installation
BCD-16521 ...................................... Front Label
Assembly Drawings
BCD-16633 ...................................... Base Stand Assembly
BCD-16301 ...................................... Control Center and Front Decorative Panel Assembly
BCD-16376 ...................................... Sliding Window Assembly and Adjustment
BCD-16299 ...................................... Base Cabinet Assembly
Electrical Schematics
CD-0000104 (sheet 1 and 2)............ NU-540-300E/400E/500E/600E Electrical Schematic
Page 5
OM0280 Page 5 of 63
Rev 1 October/2021
LabGard® ES Energy Saver
Class II Laminar Flow
Biosafety Cabinet
Models
NU-540-300E/400E/500E/600E
Manufactured by:
NuAire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The LabGard® ES Model NU-540E Laminar Flow Biosafety Cabinet (LFBSC) is a bench/table top model, optionally available
with a base support stand, for operation as a console model. The LabGard® ES model NU-540E utilizes an Energy Saver DC
ECM motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and
filter loading capacity. The Energy Saver ECM motor is controlled by the Aeromax™ Control System to nominal airflow
setpoints via an integrated solid-state DC motor controller based on feedback algorithm to provide automatic
compensation (constant volume control) for both filter loading and line voltage variances. Airflow velocity is monitored by
the PresurFlow™ system with a digital pressure sensor.
The Laminar Flow Biosafety Cabinet, (LFBSC) is a product resulting from the development of the "laminar flow" principle
(see Airflow Schematic) and the application of environmental controls as required in the field of biological research or
chemical containment. The LFBSC, when used with proper technique, is an effective primary engineering control in
obtaining the optimum product quality while reducing the potential for exposure of both product and personnel to airborne
biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product
operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU-540E bench LFBSC meets the requirements of a Class II, since the cabinet conforms to the following requirements:
1. Maintains a minimum inflow velocity of 100 LFPM (.51mps) through the work access opening.
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum.
3. Discharges a percentage of air to the outside atmosphere after HEPA filtration.
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure.
Cabinets used for work with minute quantities of non-flammable or explosive volatile toxic chemicals and tracer amounts of
radionuclides required as an adjunct to microbiological studies must be exhausted through properly functioning exhaust
canopies.
Page 6
OM0280 Page 6 of 63
Rev 1 October/2021
1.2 Safety Instructions
These safety instructions describe the safety features of the LabGard® ES Model NU-540E LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly tested
before delivery. However, the cabinet may present potential hazards if it is not installed and used as instructed for its
intended purpose or is used outside of operating parameters. Therefore, the following procedures must always be
observed:
• The safety cabinet must be operated only by trained and authorized personnel.
• For any operation of this cabinet, the operator must prepare clear and concise written instructions for operating
and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular.
o which decontamination measures are to be applied for the cabinet and accessories
o which protective measures apply while specific agents are used
o which measures are to be taken in the case of an accident
• Repairs to the device must be carried out only by trained and authorized expert personnel.
• Keep these operating instructions close to the cabinet so that safety instructions and important information are
always accessible.
• Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.
1.3 Explanation of Symbols
NOTE:
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation
which, if not avoided, may result in property
Potential electrical hazard, only qualified person
to access.
Used for important information.
Biohazard
Ground, Earth
CAUTION
Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment Required.
Lead Free
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death
of serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION
!
Page 7
OM0280 Page 7 of 63
Rev 1 October/2021
Page 8
OM0280 Page 8 of 63
Rev 1 October/2021
2.0 Models and Features
The model NU-540E, LabGard® ES, Class II Laminar Flow Biosafety Cabinet is manufactured in four
sizes: 3 ft. (.9m), 4 ft. (1.2m) , 5 ft. (1.5m) , and 6 ft. (1.8m).
Page 9
OM0280 Page 9 of 63
Rev 1 October/2021
Page 10
OM0280 Page 10 of 63
Rev 1 October/2021
Page 11
OM0280 Page 11 of 63
Rev 1 October/2021
Page 12
OM0280 Page 12 of 63
Rev 1 October/2021
Page 13
OM0280 Page 13 of 63
Rev 1 October/2021
3.0 Warranty
Details regarding product warranties can be found in the published warranty data separate from this manual and included
Within the data packet sent with the unit.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your LabGard® ES cabinet arrives without damage. Motor carriers
are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can
occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LabGard® ES cabinet to
be sure that if damage has occurred, the proper claims and actions are taken immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.
Page 14
OM0280 Page 14 of 63
Rev 1 October/2021
5.0 Installation Instructions
5.1 Location
Within the laboratory, pharmacy, etc., the ideal location of the Biosafety cabinet is away from personnel traffic lanes, air
vents (in or out), doors and/or any other source of disruptive air currents.
Suggested Cabinet Location in Laboratory
THE EXHAUST FILTER AREA IS ESPECIALLY SUSCEPTIBLE TO DISRUPTIVE AIR CURRENTS FROM AIR VENTS. The exhaust
sensor for the Electronic Airflow Control System is located just above the exhaust HEPA filter and if disruptive air currents
are present, the exhaust sensor could be influenced by them, and indicate disruptive readings on the front panel display.
If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening, the potential
exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the severity of the air current.
REMEMBER: A BIOSAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.
Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for maintenance purposes.
The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes. Do not
position the cabinet to prevent access to the power cord. The power cord plug serves as the disconnect and should
remain readily accessible. If the outlet is inaccessible, such as a conduit (hardwired) connection, then an appropriate
warning label should be applied near the cabinets on/off switch to indicate the circuit breaker on the power distribution
panel should be used. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE CABINET TO THE
CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX.
If this cabinet is used in a pharmacy application, it is strongly recommended (Per OSHA, NIOSH and ASHP) that the cabinet
be exhausted to the outside. In addition, if this cabinet is used in microbiological application with minute quantities of
volatile toxic chemicals and trace amounts of radionuclides, per CDC/NIH and NSF, it is strongly recommended that the
cabinet be exhausted to the outside. NuAire offers a canopy type of exhaust transition, which will capture the exhaust
efflux from the cabinet.
Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan)
Note: The Exhaust System should be fitted with a backdraft damper to prevent reversing of airflow in the system.
NuAire only recommends a canopy or thimble transitions for exhausting applications. See separate instruction sheets for a
discussion of exhaust transitions and installation requirements.
Page 15
OM0280 Page 15 of 63
Rev 1 October/2021
5.2 Set-Up Instructions
Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually the best
procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling. It
can then be removed from the skid by removing the banding, bolts and screws holding the cabinet to the skid. It may be
necessary to remove the control center in order to gain passage through a doorway. It can easily be removed by following
the instructions on drawing BCD-16301.
It is recommended that no less than two people are present using a
lifting system for placement of the cabinet onto the base stand.
It is not recommended to manually lift the cabinet onto the base stand.
5.2.1 Base Stand Assembly
The base stand is typically shipped knocked down in a separate carton and is assembled per drawing BCD-16385
Once assembled; place the stand in approximate position (ready for cabinet placement onto the base stand). Now
lift the cabinet on top of the base stand and bolt the base stand to the cabinet using two 3/8" - 16 x 3/4" bolts and
washers provided for the front base stand tabs and two 1/4" acorn nuts for the rear weld studs. Place the cabinet
in the desired location.
5.2.2 Leveling
Using a level placed on the work tray, first adjust the leg levelers end to end and then front to back. The NSF
approved leg levelers provide a 3/4" (20mm) adjustment.
5.2.3 Bench Installation (BCD-16882)
Place the cabinet on the bench with approximately a 2" (51mm) overhang clearance for installation of the drain
valve. Place the cabinet in desired location and using RTV caulk, seal all around the base of the cabinet and the
bench. This provides a tight seal to prevent bench spills from migrating under the cabinet.
If the drain valve is desired, (NOTE, CHECK WITH YOUR SAFETY PERSONNEL FOR REGULATORY REQUIREMENTS (i.e.
locking type) OF DRAIN VALVE INSTALLATION) remove the handle from the valve stem to gain clearance for valve
body rotation. Add Loctite 242 (furnished) to the threads and rotate valve body until secure, with the valve stem
(for handle) on the left side. Re-install handle to valve stem. Adjust the cabinet on bench to provide a 2" (51mm)
overhang and seal the interface of the bench and cabinet, using RTV caulk as above.
5.2.4 Gas Service
NuAire doesn't recommend the use of natural gas within the LFBSC, but if gas service is determined to be
necessary for the application, appropriate safety measures must take place. All NuAire LFBSC's have precautionary
warning labels that say the following:
Use of explosive or flammable substances in this cabinet
should be evaluated by your appropriate safety personnel.
Once the determination has been made by the appropriate safety personnel, the application of natural gas must
be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY RECOMMENDED THAT AN
EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE LFBSC ON THE GAS SUPPLY LINE.
The gas valve, when this option is installed, will only operate or flow gas when the cabinet blower is on and no
alarm is present. A solenoid valve is installed on the gas supply line for this purpose.
Note: Some countries (i.e. Germany) only allow certain types of certified valves to be used for natural gas
(i.e. Germany DVGW Certified). Verify requirements per Local, State and Federal codes/laws.
As previously stated NuAire doesn't recommend the use of natural gas within the LFBSC and ASSUMES NO
RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the LFBSC not only
contributes to heat build-up; it also disrupts the laminar air stream, which must be maintained for maximum
efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS
STRONGLY RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. During use, the Bunsen burner
should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect.
CAUTION
!
CAUTION
!
Page 16
OM0280 Page 16 of 63
Rev 1 October/2021
Page 17
OM0280 Page 17 of 63
Rev 1 October/2021
5.2.5 Plumbing Services
Service ball valves with the type of service specified by the removable button on the handle are located in the
work zone. The service ball valves are not recommended for pressure over 75 p.s.i. (5.2 BAR). Reducing valves
should be installed external to the cabinet if necessary. Service ball valves should never be used for flammable
gasses or oxygen service. A special needle valve for oxygen service or certified valve is required and available upon
request.
External connection is to 3/8 inch NPT coupling in the inner sidewalls. Connection to plant utilities should be made
with proper materials for the individual service and according to national and/or local codes. Observe all labels
pertaining to the type of service and operating pressure.
5.2.6 Electrical Services
The NU-540E series Biosafety Cabinets may be "hardwired" (optional) or plugged into an outlet with protective
earthing connection with the standard power cord. The cabinet requires 230 VAC, 50/60 Hz, single phase (current
rating varies per cabinet size, reference Electrical/Environmental Requirements). It is recommended that power to
the cabinet, whether hardwired or plug connected, be on its own branch circuit, protected with a circuit breaker at
the distribution panel near the cabinet.
5.2.7 Final Assembly
Remove the protective cardboard cover over the exhaust HEPA filter located under the protective screen if in
place. The exterior surface and viewing glass are easily cleaned with any mild household detergent cleaner using a
soft cloth. Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used.
Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any
mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for 60 minutes before
using it as a LFBSC.
5.3 Testing Methods and Equipment
After installation and prior to use, NuAire recommends that the cabinet be tested or commissioned to factory standards.
As part of testing, the certifier should go through the following initial checklist to assure all aspects of the LFBSC installation
are complete and ready for testing.
• Review product installation
- Exhaust connection, if present
- Damper valve installed correctly with label toward front, if present
- LFBSC base stand level
• Perform LFBSC certification
- At a minimum, the following tests should be performed:
• HEPA filter leak test
• Downflow velocity test
• Inflow velocity test
• Airflow smoke patterns
• Site installation assessment tests
The testing methods and equipment required are specified on the factory inspection report included with this manual (see
insert in back cover).
NOTE: IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR
WITH THE METHODS AND PROCEDURES FOR TESTING BIOSAFETY CABINETS (SEE INSERT).
NOTE: AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A
MINIMUM OF AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE, MAINTENANCE ACTION, OR ANY
TIME THE OPERATOR FEELS IT IS NECESSARY.
Note that the LabGard® ES cabinets, filters and seals provide premium performance; Quality Control in both design and
manufacturing assure superior reliability. However, protection to both product and operator is so vital that installation
testing to the performance requirements should be accomplished as stated to ensure Biosafety established by the factory
standards.
Page 18
OM0280 Page 18 of 63
Rev 1 October/2021
LabGard™ ES Energy Saver Class II Laminar Flow Biosafety Cabinet
Models NU-540-300E/400E/500E/600E
Catalog Number
Catalog Number
NU-540-300E
Nominal 3 foot (0.9m)
NU-540-400E
Nominal 4 foot (1.2m)
NU-540-500E
Nominal 5 foot (1.5m)
NU-540-600E
Nominal 6 foot (1.8m)
Performance Specifications
1. Personal Protection
2. Product Protection
NSF/ANSI 49
EN 12469
NSF/ANSI 49
EN 12469
NSF/ANSI 49
EN 12469
NSF/ANSI 49
EN 12469
NSF/ANSI 49
Class II
Class II
Class II
Class II
Style of Cabinet
Bench top/console
w/base stand/storage
cabinet
Bench top/console
w/base stand/storage
cabinet
Bench top/console
w/base stand/storage
cabinet
Bench top/console
w/base stand/storage
cabinet
Cabinet Construction
All welded stainless steel
16/18 GA, Type 304
pressure tight design
All welded stainless steel
16/18 GA, Type 304
pressure tight design
All welded stainless steel
16/18 GA, Type 304
pressure tight design
All welded stainless steel
16/18 GA, Type 304
pressure tight design
Diffuser for Air Supply (Metal)
Non-flammable
Non-flammable
Non-flammable
Non-flammable
HEPA Filter Seal Type:
Supply Filter-99.99% Eff. on 0.3 microns
Exhaust Filter-99.99% Eff. on 0.3 microns
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
HEPEX Seal
Neoprene, Spring-loaded
Fumigation : per NIH/NSF Procedures
Yes
Yes
Yes
Yes
Standard Services:
Service Coupling (3/8 inch NPT)
Service Coupling (3/8 inch NPT)
Gas Valve/Service Coupling (3/8inch NPT)
Outlet
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
One Right Sidewall
Two Left Sidewall
One Right Sidewall
Two Backwall
Optional Services:
Gas Cocks 3/8" NPT
Ultraviolet Light
Standard/Cup Sinks
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Up to 3 ea. Sidewall
One, Backwall
Left or Right Work
Surface
Cabinet Size Inches (mm):
Height (Fully Assembled)
Height (Minimum for Transport)
Width
Depth with Armrest Removed
60 7/8 (1546)
59 (1499)
41 5/8 (1057)
31 7/16 (799)
60 7/8 (1546)
59 (1499)
53 5/8 (1362)
31 7/16 (799)
60 7/8 (1546)
59 (1499)
65 5/8 (1669)
31 7/16 (799)
60 7/8 (1546)
59 (1499)
77 5/8 (1972)
31 7/16 (799)
Work Access Opening Inches (mm):
Standard Opening Height
Standard Inflow Velocity
10 (254)
105 FPM (.53 m/s)
10 (254)
105 FPM (.53 m/s)
10 (254)
105 FPM (.53 m/s)
10 (254)
105 FPM (.53 m/s)
Work Zone Inches (mm):
Height
Width
Depth measured at 10 inches (254mm)
window height
28 1/2 (724)
34 3/8 (873)
25 3/4 (654)
28 1/2 (724)
46 3/8 (1178)
25 3/4 (654)
28 1/2 (724)
58 3/8 (1483)
25 3/4 (654)
28 1/2 (724)
70 3/8 (1788)
25 3/4 (654)
Viewing Window Inches (mm):
Standard is safety plate sliding glass
Fully closed to
21 (533) open
Fully closed to
21 (533) open
Fully closed to
21 (533) open
Fully closed to
21 (533) open
Required Exhaust CFM/CMH
Standard/Optional:
Canopy Variable Flow Thimble (NU-911)
Canopy Fixed Flow Thimble (NU-907)
10(254)
CFM (CMH)
276-501 (649-851)
320 (544)
10(254)
CFM (CMH)
363-588 (617-1000)
426 (724)
10(254)
CFM (CMH)
451-676 (766-1149)
531 (902)
10(254)
CFM (CMH)
538-763 (915-1297)
634 (1077)
Plant Duct Static Pressure Eng./Metric
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
0.05-0.1"/1.27-2.54mm
H2O
Heat Rejected, BTU, Per Hour
(non-vented)
(vented)
10(254) opening
903
120
10(254) opening
1140
157
10(254) opening
1768
198
10(254) opening
1884
198
Electrical: CE marked:
Volts, AC 50/60 Hz
+Amps: Blower/Lights (10 openings)
Amps: Outlet
Rated Amps:
12 ft. Power Cord (one)
230
2.3
3
8
14 GA - 3 Wire, 15A
230
2.9
3
10
14 GA - 3 Wire, 15A
230
4.5
3
11
14 GA-3 Wire, 15A Std.
230
4.8
3
11
14 GA-3 Wire, 15A Std.
Crated Shipping Weight:***
Net Weight
450 lbs. /204 kg.
400 lbs. /181 kg.
515 lbs. /234 kg.
465 lbs. /211 kg.
600 lbs. /272 kg.
550 lbs. /249 kg.
670 lbs. /304 kg.
620 lbs. /281 kg.
Sound Pressure Level per ISO 4871****
Not to Exceed 55 dbA ■
Not to Exceed 56 dbA ■
Not to Exceed 58 dbA ■
Not to Exceed 60 dbA ■
***Crated shipping weight does not include weight for accessories or options
+ Based on cabinet with new filters running at 230VAC.
****Uncertainty is K = 2 dbA, measurement performed per ISO 11201 in normal running mode.
■Reference the customer test report for procedure and results.
Page 19
OM0280 Page 19 of 63
Rev 1 October/2021
6.0 Operating the NU-540E
6.1 Aeromax™ Control System
6.1.1 Overview
The Aeromax™ control system is designed to service the control requirements of the LabGard® ES NU-540E
Biosafety Cabinet. The Aeromax™ control system consists of an electronic module that will perform the following
functions:
• Easy user interface via LED’s and function keys
• Control blower via solid state switch.
• Control lights via solid state switch.
• Control outlets via solid state switch.
• Disable audible alarm switch with ring back function.
• Control blower DC ECM motor with solid-state DC Motor Controller that provides automatic
compensation (constant volume control) for both filter loading and line voltage variances.
• Monitor and display airflow system performance via PresurFlow™ monitor.
The LabGard® ES NU-540E offers the latest digital microprocessor design technology for improved cabinet
performance and safety. The Aeromax™ control system integrates a digital pressure sensor (PresurFlow™) to
monitor the cabinet’s airflow performance. The Aeromax™ control system also integrates a DC motor controller
that provides automatic compensation for both filter loading and line voltage variances. There is additional on/off
control of blower, LED light, ultraviolet light (optional) and outlets. Lastly the Aeromax™ control system monitors
the sliding window position with micro switches. All the above functions are shown in a system block diagram (see
figure 1).
Figure 1
Page 20
OM0280 Page 20 of 63
Rev 1 October/2021
Page 21
OM0280 Page 21 of 63
Rev 1 October/2021
6.1.2 Front Panel
The control system front panel contains the following functions described in detail (see Drawing BCD-16521).
6.1.2.1 Blower Keys
The blower key controls the ON/OFF power to the blower.
LED above key indicates: full green for blower on,
blinking green for blower pending and
full red for blower alarm.
6.1.2.2 Hidden Key
The hidden key is located just above the blower LED indicator centered in the airflow symbol. The hidden key
is used for various functions including the blower password 3 key sequence if the option is activated.
6.1.2.3 LED Light Key
The LED light key controls the on/off power to the LED light.
LED above the key indicates full blue for LED light on.
6.1.2.4 Ultraviolet (UV) Light Key
The UV light key controls the on/off power to the UV light (if optionally installed).
LED above indicates full yellow for UV light on.
6.1.2.5 Outlet Keys
The outlet key controls the ON/OFF power to the outlets.
LED above indicates full blue for outlets on.
6.1.2.6 Red Alarm LED
The red alarm LED will indicate any alarm condition and remain indicating until the alarm condition is cleared.
6.1.2.7 Audible Alarm Silence
The audible alarm silence key allows user interaction to silence an audible alarm for a period of 15 minutes.
After 15 minutes if the alarm condition still exists, the audible alarm will again sound.
The audible alarm silence key also is used to exit all FlowGard™ user interaction menus.
6.1.2.8 Arrow Adjustment Keys
The arrow adjustment keys allow user interaction for various functions.
Page 22
OM0280 Page 22 of 63
Rev 1 October/2021
6.1.3 Aeromax™ Control System Power
After the LabGard® ES NU-540E is plugged into the appropriate facility line power the control system will power
up. The control panel will also indicate the power up status by blinking the red alarm LED. Pressing any key will
acknowledge the power up status and turn off the blinking red alarm LED.
If a power interruption occurs, all control system functions, calibrations and parameters will be maintained and
continue upon restoration of power. Just as the initial power up, the red alarm LED will blink to indicate power up
status.
6.1.4 Standby Mode
When the BSC is not in use any of the function keys except the blower that initiates run mode may be turned on
and off in standby mode.
6.1.5 Run Mode
Any time the blower run key is pressed with the sliding window at its correct operational height, the RUN MODE
screen will be initiated. The Run Mode will start with the PresurFlow™ entering and approximate 3 minute warm
up period. The PresurFlow™ LED indicators will blink and indicate the following sequence:
• 1st minute – Left and right Red LED’s will blink
• 2nd minute – Left and right Green LED’s will blink
• 3rd minute – Center 3 Green LED’s will blink
Once the warm up period is complete, only one LED will indicate cabinet airflow status.
During the warm up period the aseptic cleaning process may begin. If the sliding window is raised, an audible and
visual alarm will occur, but may be silenced by pressing the alarm silence key.
6.1.6 Nite Care Mode
The NU-540E may be configured to allow the DC ECM motor to continue to run at a lower rate with the sliding
window closed allowing the workzone interior to be continually HEPA filtered. If the Nite Care mode is configured,
the blower must be on (green LED above blower key will blink) and the window closed for it to be activated. Once
activated, blower LED indicator will blink fast and the PresurFlow™ will indicate 3 Green LED indicators will blink.
In addition, the LED light will be disabled. The UV light may be turned on if installed.
6.1.7 Standby/Run Mode Alarms
If present, standby/run mode alarms will be both visual and audible, the red alarm LED oval will turn on. Audible
alarms will produce an alarm tone for 30 seconds, then ring back for 2 seconds of every 5 seconds. Pressing the
alarm silence key will silence the audible alarm for 15 minutes initially then will start the ring back function again.
The list below represents alarm types and their respective priority from the highest to lowest priority.
1) New Firmware Loaded
2) Internal Board Failure
3) Power on Reset
4) Airflow Pressure Alarm
5) Blower RPM Failure
6) Window High
7) Window Low
8) Replace UV Light
Note: The above messages are described in greater detail in section 8.
Page 23
OM0280 Page 23 of 63
Rev 1 October/2021
6.1.8 Operator Accessible Functions
6.1.8.1 Access and Navigation
To access the operator accessible functions,
• Press and hold the key, then enter the 3 key sequence for the desired function, then release
the key and follow each instruction set.
Note: Pressing the key at any time will abort and exit the process without saving any changes
made. Pressing the hidden key will accept all changes and exit.
6.1.8.2 Auto Timer Duration
Auto timer duration timers are countdown timers for the functions displayed once time is entered into a
function. The timer will begin to countdown upon the start of that function (i.e. press UV light key to start
timing the UV light). The LED indicator above the function key will start to blink indicating the timer function.
If the LED indicator was full on, no timer function is present. As the timer expires the function will turn off.
• Select auto timer duration function
o Outlets
Press and hold key, then press hidden – outlet – outlet keys sequentially.
LED indicator above outlet will blink fast. Adjust desired time as described below.
o Lights
Press and hold key, then press hidden – light – light keys sequentially.
LED indicator above light will blink fast. Adjust desired time as described below.
o UV Light
Press and hold key, then press hidden – UV light – UV light keys sequentially.
LED indicator above UV light will blink fast.
Adjust desired time as described below.
o Nite Care Blower
Press and hold key, then press hidden-blower-blower keys sequentially.
LED indicator above Blower key will blink fast. Adjust desired time as described below.
o Adjust countdown time
Press ↑ or ↓ keys to adjust time.
Time will change in 15 minute increments as shown on the PresurFlow™ LED segments
below.
15 minutes for Red LED 30 Minutes for Yellow LED
1 hour for green LED Represents 8 hours (maximum time)
• Press hidden key to accept time and exit.
• Press key at any time to abort and exit.
Page 24
OM0280 Page 24 of 63
Rev 1 October/2021
6.1.8.3 Blower Password
The blower on/off password allows the cabinet user to place a 3 key sequence requirement to turn the blower
on or off.
The 3 key sequence for the blower password will be a combination of the hidden and blower keys.
• Select blower password
Press and hold key, then press hidden – blower – hidden keys sequentially.
Red LED indicator above blower will blink fast.
• Select password
Press ↑ or ↓ key to scroll through the code choices below,
No Password
B-B-H password
B-H-B password (default)
B-H-H password only
H-B-B password
H-B-H password
H-H-B password
• Press hidden key to accept time and exit.
• Press key at any time to abort and exit.
Note: If the required blower password option is selected in the blower airflow option menu (see section 8.2.2).
Then the “No password” choice above is not available and the default remains B-H-B.
Page 25
OM0280 Page 25 of 63
Rev 1 October/2021
6.2 Operating Guidelines
The intent herein is to present general operational guidelines that will aid in the use of the Laminar Flow Biosafety Cabinet
(LFBSC) to control airborne contaminants of low to moderate risk as stated in Technical Report No.
FPS 56500000001, prepared by Dow Chemical U.S.A. for the National Cancer Institute, May 1, 1972.
Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be developed in order to
obtain a maximum potential for safety and protection. The pre-planning necessary to develop these protocols is based on
several fundamental considerations, each of which will contribute to optimum benefits from the equipment:
a. Know your “Safe Work Area”
b. Minimize penetration of “air curtain”
c. Minimize room activity
d. Utilize unidirectional airflow
e. Employ aseptic techniques
6.2.1 Know your "Safe Working Area"
The LFBSC safe working area is basically the worktray or depressed area. All work should be performed on or
above the worktray. The area on or above the front grill is a non-safe working area.
NOTE: It is important to maintain an air gap on both sides of the worktray before fastening in place.
The work tray as being part of the cabinet system has been designed to load up to 100 lbs. (45.4kg) of work
materials. Any additional loading should be evaluated by appropriate safety personnel.
6.2.2 Minimize Penetration of "Air Curtain"
The minimum number of items necessary should be placed into the cabinet to prevent overloading, but the work
should also be planned to minimize the number of times an operator's hands and arms must enter and leave the
air curtain at the open face. The ideal situation is to have everything needed for the complete procedure placed in
the hood before starting, so that nothing need pass in or out through the air barrier at the face until the procedure
is completed. This is especially important in working with moderate risk agents.
Avoid lifting arms/hands inside the cabinet above the level of the work opening. This presents an inclined plane
from hands to elbows along which the downflow of air may run to, and possibly out, the open face.
NOTE: When working with agents of lower risk, it is not as important for all materials to be placed in the cabinet before
starting or for the procedure to be completely finished before materials are removed.
Also, the time period for the cabinet may be continued over a longer extended period, during which entries and
withdrawals from the cabinet may be made.
6.2.3 Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air currents as
well as interfere with the work of the operator. A person walking past the front of a cabinet can cause draft
velocities up to 175 fpm (.89 m/s), which are sufficient to disrupt the air balance of the LFBSC.
Page 26
OM0280 Page 26 of 63
Rev 1 October/2021
6.2.4 Utilize Unidirectional Air Flow
The operator must keep two important facts in mind: (1) The air, as supplied to the work area through filters from
the top, is contaminant free and (2) Airborne contamination generated in the work area is controlled by the
unidirectional flow of parallel air streams in a top-to-bottom direction.
A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability of
controlling lateral movement of airborne particulates. A cone of turbulence extends below the object and
laminarity of the air stream is not regained until a point is reached downstream, approximately equal to three to
six times the diameter of the object. Within the parameters of this cone, particles may be carried laterally by
multidirectional eddy currents.
Transfer of viable materials and manipulations, which may generate aerosols, should not be performed above
sterile or uninoculated materials. Items should be localized on the work surface in "clean" and "dirty" groups.
6.2.5 Employ Aseptic Technique
The operator must not assume an attitude of "let the cabinet do it" when performing procedures within a LFBSC.
Properly balanced and properly used cabinets will do an excellent job of controlling airborne contamination and
containing viable agents, but the cabinet will not eliminate contact transmission of contamination. Normal
laboratory contamination control procedures and basic aseptic techniques are necessary to obtain maximum
benefit from the cabinet. For example, open bottle, tube or flask mounts should be kept as parallel as possible to
the downflow to minimize capture of chance particulates. This precaution is merely an extension of good aseptic
technique as practiced on open bench tops. The standard laboratory practices designed to minimize creation
and/or release of aerosols to the environment should not be discontinued.
Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or until
surface-decontaminated. Trays of discard pipettes must be covered before removal from the cabinet (aluminum
foil may substitute for fabricated covers).
If an accident occurs which spills or splatters suspensions of etiologic agent around the work area, all surfaces and
items in the cabinet must be surface-decontaminated before being removed.
Applying a burner flame to flask and tube necks when mating surfaces of sterile assemblies is a conventional
method of minimizing chance contamination. However, the efficiency of this operation is usually related to the
removal of airborne contamination occurring while the item is uncovered. If the manipulation is carried out in an
environment free of airborne particulates, then the need for the flaming operation is essentially removed. This is
one of the additional advantages of the LFBSC - use of the gas burner is seldom necessary. The gas burner flame in
one of these cabinets not only contributes significantly to the heat build-up, it also disrupts the laminar air streams
which must be maintained for maximum efficiency. If the procedure demands use of a flame, A BUNSEN BURNER
WITH ON DEMAND IGNITION IS RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. It should also be
only used from the center of the work surface to the right rear where resulting air turbulence will have a minimal
effect. DO NOT USE GAS BURNER ON THE LEFT SIDE OF THE WORK SURFACE DUE TO ITS INFLUENCE ON THE
ELECTRONIC AIRFLOW CONTROL SYSTEM. If cabinet air is inadvertently turned off, the flame could damage the
HEPA filters.
Page 27
OM0280 Page 27 of 63
Rev 1 October/2021
6.3 Operating Sequence
6.3.1 Start Up
Turn on cabinet blower and lights and check air intake and exhaust portals of the cabinet to make sure they are
unobstructed. The electronic airflow control system will automatically control airflows to specified setpoints.
However, upon filter loading, the cabinet may be required to be re-balanced or filters replaced. Only a qualified
maintenance technician should perform cabinet balancing and filter replacement.
NOTE: Some cabinets are equipped with ultraviolet (UV) lights. Good procedure includes the decontamination or wipe
down of cabinet surfaces with chemical disinfectant before work commences. This practice eliminates the need
for UV lights, whose primary utility in this application is inactivation of surface contamination since the filters
effectively remove all airborne contaminants. UV lights, therefore, are not recommended in the LFBSC.
Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun in the cabinet. If
the filtered air exhausted from the cabinet is discharged into the room, as in some installations, an additional
advantage is obtained from purification (filtration) of the room air circulated through the equipment. Because of
this characteristic contributing to the quality of the laboratory environment, some owners of LFBSC’s leave them in
operation beyond the time of actual use.
6.3.2 Wipe Down
The interior surfaces of the workspace should next be disinfected (see Cleaning Procedures) by wiping them
thoroughly with 70% alcohol or similar non-corrosive anti-microbial agents. USE OF CHLORINATED OR HALOGEN
MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL.
6.3.3 Materials and Equipment
The apparatus and materials should next be placed into the cabinet. Care must be exercised that no items be
placed over the front intake grills. Materials should be arranged so that clean, dirty (used), and virus materials are
well separated. Passage of contaminated materials over uninoculated cultures or clean glassware should be
avoided and transfer of viable materials should be performed as deeply into the cabinet (away from open face) as
possible.
6.3.4 Air Purge
Additional purging of the workspace without user activity should be allowed for 2-3 minutes after materials and
apparatus have been placed in it. This will rid the area of all "loose" contamination that may have been introduced
with the items.
6.3.5 Perform Work
The work can now be performed. The technician performing the work is encouraged to wear a long-sleeved gown
with knit cuffs and rubber gloves. This will minimize the shedding of skin flora into the work area and concurrently
protect the hands and arms from viable agent contamination. At a minimum, the hands and arms should be washed
well with germicidal soap before and after work in the cabinet. For the preparation of antineoplastic drugs, the
following procedures summarize those contained in OSHA’s guidelines to Controlling Occupational Exposure to
Hazardous Drugs. The OSHA document should be thoroughly studied and reviewed prior to drug preparation in the
cabinet. It may be found at this website:
https://www.osha.gov/SLTC/hazardousdrugs/controlling_occex_hazardousdrugs.html
a. A sterile plastic-backed absorbent drape should be placed on the work surface during mixing procedures.
The drape should be exchanged whenever significant spillage occurs, or at the end of each production
sequence.
b. Vials should be vented with a filter needle to eliminate internal pressure or vacuum.
c. Before opening ampoules, care should be taken to insure that no liquid remains in the tip of the ampoule.
A sterile gauze sponge should be wrapped around the neck of the ampoule while opening.
d. Final drug measurement should be performed prior to removing the needle from the stopper of the vial.
e. A non-splash collection vessel should be available in the LFBSC to discard excess drug solutions.
Page 28
OM0280 Page 28 of 63
Rev 1 October/2021
6.3.6 Terminal Purging and Wipe Down
Following completion of work, allow the cabinet to run for 2-3 minute period without personnel activity to purge
the cabinet. A surface disinfection of the interior surfaces (see Cleaning Procedures section) should be repeated
after removal of all materials, cultures, apparatus, etc. A careful check of grills and diffuser grids should be made
for spilled or splashed nutrients which may support fungus growth and resulting spore liberation that
contaminates the protected work environment.
6.3.7 Paper Catch/Prefilter
A permanent paper catch is installed behind the rear divider panel of the work zone. This area forms the return air
path to the motor/blower; and if the airflow is blocked, it could seriously affect the performance of the cabinet.
Therefore, THE PAPER CATCH SHOULD BE CHECKED AND CLEANED ON A MINIMUM OF A WEEKLY BASIS. A daily
basis if procedures dictate the use of paper products. Any paper removed must be properly disposed of as
Contaminated Hazardous Waste. The above procedures also apply to all cabinets configured with a prefilter.
6.3.8 Shut Down
Turn off blowers and lights. Do not use cabinet as a depository for excess lab equipment during periods of nonoperation. If antineoplastic agents are being prepared in the cabinet, it is recommended to let the cabinet run 24
hours per day. This lessens the possibility that contaminants may escape.
6.4 Ergonomics
Ergonomics, the study or accommodation of work practices is extremely important for proper cabinet usage and user
health and safety. An evaluation of normal work practices should be performed with each user when working in a cabinet.
Evaluation criteria should be at a minimum:
a. Proper user posture
b. Effective work zone layout for work practice
c. Vision or sightlines
For each of the above evaluation criterion, several work aids may be supplied to accommodate the user.
• Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to assure proper user
posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure back is fully supported
with proper chair adjustments.
• Forearm/elbow support - The cabinet is provided with a non-metallic forearm support on the work access opening.
Periodic mini-breaks during work practice should be taken resting forearm to avoid stress and fatigue. Elbow rests
that can provide support for particular work practices, such as pipetting are optional. Also available as an option,
closed cell foam disposable forearm pads to reduce pressure points and add comfort.
• Effective workzone layout - Always prepare your work procedure to minimize reach to avoid neck and shoulder
stress and fatigue. Rotating tables used to maximum workzone and minimize reach are optional.
• Vision and sightline - Always prepare your work procedure to eliminate glare and bright reflections on the window.
Keep your window clean and sightlines clear to your effective workzone.
6.5 Cleaning Procedures
6.5.1 General
Cleaning laboratory equipment is important in terms of both functionality and general good housekeeping. The
information provided below is intended to aid the development of facility Standard Operating Procedures (SOP’s)
for cleaning the equipment. It is strongly recommended that all cleaning materials used be tested and verified in
terms of both effectiveness and material compatibility before they are written into the cleaning SOP
documentation.
a. The airflow blower should be operating during the cleaning process to maintain sterility and/or containment
during the cleaning process.
b. Raise window to gain additional access if desired.
c. Apply appropriate cleaning material or surface disinfectant to surfaces. Most surface disinfectants require a
specific contact time depending the materials used within the work zone. CONSULT APPROPRIATE DISINFECTANT
DOCUMENTATION FOR PROPER APPLICATION AND SAFETY PRECAUTIONS.
c-1. Stainless steel (type 304) has noted material compatibility concerns with Acids, Chlorides and Halogens. IF
THESE MATERIALS ARE USED AND ALLOWED TO BE LEFT ON THE STAINLESS STEEL SURFACE, OXIDATION AND
DEGRADATION WILL OCCUR. Only by re-wiping surfaces with either sterile water or 70% IPA will remove harmful
materials from the stainless steel surface.
Page 29
OM0280 Page 29 of 63
Rev 1 October/2021
Further information is available at the following: http://www.parrinst.com/wpcontent/uploads/downloads/2011/07/Parr_Stainless-Steels-Corrosion-Info.pdf
NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR
OWN RISK! The information provided above is from raw material suppliers and known general source documents
for use to develop application cleaning SOP’s.
NOTE: When cleaning the work area for the first several times, the new metal surfaces may produce some dark
discoloration on the white cleaning wipes. Repeated cleaning will continuously reduce the amount of the
discoloration material on the cleaning wipes over time.
6.6 Hazardous Drug Decontamination Procedures
This procedure is intended to provide guidance following a spillage and/or periodic maintenance, testing or relocation of
the cabinet. Additional guidance can be provided by the CETA document CAG-005-2007 found at the CETA website:
www.CETAinternational.org.
6.6.1 Preparation
Prior to beginning decontamination activity, personnel should wear proper personnel protection equipment (PPE)
i.e. Tyvek1 isolation gown, 2 pair of Nitrile gloves and a full-faced HEPA filtered respirator. All protective garments
should be contained in 4 mil plastic bags and labeled for disposal as chemotherapy waste after completion of the
procedure. For the purpose of this procedure, detailed procedures for cleaning a Class II LFBSC can be found in the
2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs2.
6.6.2 Procedure
a. Make sure that the cabinet remains in operational mode with internal blower on.
b. Open the hinged or sliding view screen and secure in the full open position.
With the view screen in the full open position, personnel protection is
compromised and a full faced HEPA filtered respirator must be worn.
c. Clean all readily accessible surfaces of the cabinet.
d. Remove perforated metal diffuser screen from the underside of the supply HEPA filter and place on the
cabinet work tray.
Note: Depending on the model, the diffuser screen is secured to the cabinet by
#8-32 screws or 1/4" - 20 acorn nuts, 3 places. It is purposely a tight fit
and is secured to the back wall with projecting threadless studs.
e. Clean both sides of the perforated metal diffuser screen and remove it from the cabinet.
f. Lift the cabinetwork tray, clean both sides and remove it from the cabinet.
g. Remove the front perforated grill, place on the cabinet floor and clean both sides.
Remove from cabinet.
h. Clean work tray supports.
i. Working from top to bottom, clean all inside surfaces of the cabinet.
Take care not to wet the HEPA filter.
If liquid has collected in the plenum drain, aspirate it using IV tubing into an evacuated container.
Label the evacuated container for disposal as chemotherapy waste.
j. Clean the plenums drain area and wipe dry.
k. If the cabinet requires maintenance and/or replacement of the HEPA filters, the operation should be
halted at this point to allow trained personnel to complete replacement of the HEPA and/or maintenance
action required.
CAUTION
!
Page 30
OM0280 Page 30 of 63
Rev 1 October/2021
6.6.3 Assembly
a. Replace front grill (if removed).
b. Replace the work tray and carefully tighten the thumbscrews.
c. Replace perforated metal diffuser screen over the underside of the supply HEPA filter.
d. Wipe down all exposed surfaces of the work area with 70% isopropyl alcohol.
e. Prepare for aseptic operation.
¹
Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributors.
²American Society of Hospital Pharmacists. 2006. ASHP Guidelines on Handling Hazardous Drugs Am. J. Hosp. Pharm. 63:1172-1193.
7.0 General Maintenance
All maintenance actions on this equipment must be performed by a qualified
technician who is familiar with the proper maintenance procedures required
including both certification and repair.
7.1 Decontamination
No maintenance should be performed on the interior of the LabGard® ES cabinet (area behind access panels) unless the
cabinet has been microbiologically decontaminated, is known to be biologically clean, or known to be chemically inert.
Surface disinfection is performed as specified in the Cleaning Procedures section.
Hazardous Gases! Personal Protection Equipment Required.
Decontamination using hazardous gas, vapor or mist must be performed in accordance with the
specifications of NSF/ANSI 49, Annex G, EN12469, Annex J or applicable national, state, province
or local regulations.
This procedure presents considerable risks and must be performed only by specially trained and authorized service
personnel in accordance with applicable national safety regulations. (i.e. US – OSHA/NIOSH, Germany TRGS-522).
The decontaminate is generated either external or internal of the sealed cabinet. The quantity of decontaminate should
follow standard or manufacturer’s recommendations based on cabinet volume. The decontaminate process should follow
standards or manufacturer’s recommendations based on the decontaminate used.
All decontaminate materials are hazardous (chemical-liquid, gas and vapor)
(Flammable – process) and are required to be handled properly. Follow all
product and process documentation and labelling.
CAUTION
!
CAUTION
!
CAUTION
!
Page 31
OM0280 Page 31 of 63
Rev 1 October/2021
7.1.1 Manual Decon
1. Disconnect power to the cabinet. Remove screws at each upper side of the control center and allow the
control center to rotate down, resting on the safety straps. Disconnect electrical connectors on left side.
Disconnect electrical from right side. (Be sure to note the location of the supply and exhaust sensor wires
before disconnecting them from the main board). Remove control center by disconnecting safety straps
lifting the control center up and away from cabinet. Fold and pinch tubing to seal.
2. Remove the front decorative panel via top/front fasteners.
3. Remove window assembly (both frame and window, either being manual, sliding, or automatic) via the
upper and lower fasteners (Refer to BCD-16376). At this point, the sliding window assembly can be
removed.
4. Remove exhaust sensor shrouds.
5. Remove the diffuser screen and gasket around perimeter of workzone, if present.
6. Prepare decontamination equipment. Reference decontamination procedure and use the following chart
to calculate chemical requirements.
Note: The outlets in the work area are energized as long as the cabinet is plugged in and
switched on the front panel. Unplug the cabinet before decontamination equipment is
plugged into these outlets. The control centers electrical connectors may be re-attached
to utilize the cabinet's interior outlets and fan during the decontamination.
7. Set control center on its side resting on the left side of cabinet. Then plug in the 16-pin connector cable
from the cabinet to the control center. Reconnect power to the cabinet. The outlets and blower can then
be activated.
8. Seal front and top openings using plastic and tape.
BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY
LABORATORY EXPOSURE TO DECONTAMINATION GAS.
9. Perform decontamination procedure.
Please consult with NuAire, Inc. about any unique contamination problems.
Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area behind the
access panel containing the HEPA filters and motor (blower assembly). All required adjustments in order
to maintain proper cabinet airflows are external to the cabinet interior. The motor is lubricated for life
and is thermally protected with automatic reset.
7.2 LED Lamp Replacement
The two (T8) LED bulbs are cool white and placed external to the cabinet to aid maintenance and minimize heat build-up
within the cabinet. The life rating of the bulb is 50,000 hours for LED based on three-hour burning cycles.
To replace a lamp, it is necessary to remove the lamp assembly.
1. Switch cabinet light switch off.
2. Remove the screws at each upper side of the control center and allow the control center to rotate down, resting
on the safety straps.
3. The lamp is now directly exposed for replacement.
4. The lamp is removed by displacing the bulb to one side against the compressible bulb holder and lifting out the
lamp.
5. Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps, cable or tubing
during closure of the control center.
¹
Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributor
Cabinet Size
300E
400E
500E
600E
Cabinet
Dimensions
58-7/8 x 28 x 34-3/8 in.
(1.50 x .711 x .873 m)
58-7/8 x 28 x 46-3/8
(1.50 x .711 x 1.18 m)
58-7/8 x 28 x 58-3/8
(1.50 x .711 x 1.48m)
58-7/8 x 28 x 70-3/8
(1.50 x .739 x 1.88 m)
Cabinet
Volume
32.79 cu. ft.
(.929 cu. m)
44.24 cu. ft.
(1.25 cu. m)
55.69 cu. ft.
(1.58 cu. m)
67.14 cu. ft.
(1.90 cu. m)
CAUTION
!
Page 32
OM0280 Page 32 of 63
Rev 1 October/2021
7.3 HEPA Filter/Motor Replacement
The HEPA Filters do not need replacement under normal usage and barring an accident (a puncture), until the efflux
velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM (.51 m/s) (min.). This may
permit the average downflow velocity to be as low as 55 LFPM (.28 m/s) as long as no point falls below 20% of the average
downflow velocity.
The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to be biologically
"clean".
7.3.1 Procedure
Disconnect electrical power from the cabinet before attempting any
maintenance action.
Step 1: Remove screws at each upper side of the control center and allow the control center to rotate down,
resting on the safety straps. Second, remove the front decorative panel which is held into position by
(3) knurled nuts on the top edge and snap fit bullet catches on the bottom.
Step 2: Place sliding window into lowest position * and remove front filter panel, which is held into position
by Phillip pan head screws. Once the screws are removed, the panel is held into position by smooth
weld studs located on the top corner of the front filter panel. Use the window stop brackets as
handles to remove the panel.
Note: Screws are used in lieu of acorn nuts, and lock washers.
The screws have O-rings and should be replaced if damaged or badly deformed.
The interior of the cabinet is now fully exposed for replacement of the filters and motor/blower.
* The lowest window position will require the armrest to be removed.
Step 3: Filter Removal
It is not always necessary to replace both the supply and exhaust filters at the same time. If during
the course of certifications, the downflow always falls off while the exhaust increases the supply filter
is "loading" faster than the exhaust filter and only the supply filter may need replacement. The
opposite might also happen depending upon many factors.
a. To remove the supply HEPA filter:
1. Remove the plenum screws in front of the supply HEPA filter.
2. Lift the permanent plenum and hold up with wire strap.
3. Carefully remove the supply filter. Direct exposure should be avoided.
Dispose of spent HEPA filters properly. Avoid direct contact to "dirty
side" of the filters. Place in sealed bag and label all waste
containers/cartons based on type of hazard. Follow all Local, State
and Federal guidelines for disposal of HEPA filter solid waste.
b. To remove the exhaust HEPA filter:
1. Relax the exhaust filter seal loading mechanism by turning the four threaded bolts
counterclockwise until one can see a definite release of the loading springs.
2. Pull the exhaust choke tray free and remove the filter. It is not necessary to remove
the tray, although it is free to move forward several inches, if necessary, to free the
HEPA filter.
CAUTION
!
CAUTION
!
Page 33
OM0280 Page 33 of 63
Rev 1 October/2021
Step 4: Filter Installation
When installing new filters, USE ONLY NUAIRE SPECFIED FILTERS FOR REPLACEMENT.
Description:
Supply HEPA Filter
Exhaust HEPA Filter
Efficiency:
99.99% @ 0.3 Micron
99.99% @ 0.3 Micron
Airflow Rating:
100 fpm @ .55 .05" w.g. per sq. ft.
250 fpm @ .23 .05" w.g. per sq. ft.
Frame Type:
Metal
Metal
NU-540-300E
NuAire Part Number:
A-980979-04
A-980957-17
Filter Size:
21" (533mm) x 32" (813mm) x 3" (76mm)
20" (508mm) x 14" (366mm) x 6" (152mm)
Filter Manufacturer:
Camfil Farr
Camfil Farr
NU-540-400E
NuAire Part Number:
A-980979-01
A-980957-18
Filter Size:
21" (533mm) x 44" (1118mm) x 3" (76mm)
20" (508mm) x 22” (559mm) x 6" (152mm)
Filter Manufacturer:
Camfil Farr
Camfil Farr
NU-540-500E
NuAire Part Number:
A-980979-02
A-980957-19
Filter Size:
21" (533mm) x 56" (1422mm) x 3" (76mm)
20" (508mm) x 30" (762mm) x 6" (152mm)
Filter Manufacturer:
Camfil Farr
Camfil Farr
NU-540-600E
NuAire Part Number:
A-980979-03
A-980957-20
Filter Size:
21" (533mm) x 68" (1727mm) x 3" (76mm)
20" (508mm) x 36" (914mm) x 6" (152mm)
Filter Manufacturer:
Camfil Farr
Camfil Farr
Page 34
OM0280 Page 34 of 63
Rev 1 October/2021
a. To install the supply filter, simply reverse the procedure outlines in Step 3a, above.
Note: Be sure to open the choke plate fully before inserting the filter into the tray.
This will assist in adjusting the airflow.
b. To install the exhaust filter, apply a thin layer of silicone grease to the top and bottom
gaskets of the filter and carefully insert into the exhaust choke tray.
Position the filter frame within the outside walls of the exhaust opening on the top of the hood.
Tighten the spring loaded bolts, 4 places, depressing the gasket material by 1/8 inch (3mm)
Step 5: Motor/Blower Assembly Removal
a. It is recommended that the motor/blower to be removed as a single unit. To remove,
disconnect electrical connections to the motor, remove the HEPEX pressure plenum and
unbolt the motor/blower assembly from the roof of the cabinet (4 places). Always inspect
the rubber isolation motor mounts and replace those that are cracked or visibly show stress.
b. Replace the motor exactly as originally installed in the blower housing, paying particular
attention to the correct electrical connections (see Electrical Schematic).
c. Re-install the new motor/blower assembly.
Page 35
OM0280 Page 35 of 63
Rev 1 October/2021
7.4 Sliding Window Replacement and Manual Adjustment
The sliding window replacement is accomplished by removing the front decorative panel, control center, and window glide
assemblies. The sliding window adjustment may be required due to everyday use over the life of the cabinet. Both window
glides are adjustable by a set screw and tension screw method. When adjusting the sliding window, be sure to verify
proper micro switch operation. If the sliding window is too loose, it will not properly activate the micro switches, thus
causing potential operational malfunctions to occur. If the window counter-balance experiences a fault, window function
must be verified (the sliding window retention or ability to slow the rate of fall). See Sliding Window Retention Verification
in the Inspection Report).
7.5 Airflow Control System Setup and Calibration
7.5.1 General
The operation of the NU-540E cabinet requires that the setup and calibration procedures be performed in order to
certify or commission the cabinet for usage. The setup and calibration procedures performed ONLY BY THE
CABINET CERTIFIER ensure that cabinet's setpoints are verified and that the airflow monitor sensor is calibrated to
the correct values.
Page 36
OM0280 Page 36 of 63
Rev 1 October/2021
7.5.2 Configuration Parameters
Configuration parameters identify cabinet motor type and size for proper performance characteristics.
• Select/Verify cabinet model and size (Bold items represent default parameters)
o Press and hold key, then press blower – blower – blower keys sequentially.
LED indicates above both blower (red) and UV light keys will blink fast.
Review cabinet model size and change if desired as described below
o Press ↑ or↓ keys to scroll through the LED segment choices associated with model/size.
Class II, 3 foot
Class II, 4 foot
Class II, 5 foot
Class II, 6 foot
Laminar, 3/4 foot
Laminar, 5/6 foot
o Press hidden key to accept model/size and exit
o Press key at any time to abort and exit
• Select motor control function/type
o Press and hold key, then press light – light – light keys sequentially.
LED indicators above both blower (red) and light keys will blink fast.
Review motor control function/type and change it desired as described below.
o Press ↑ or ↓ keys to scroll through the LED segment choices associated with motor control
function/type.
Auto DC ECM motor
Fixed duty DC ECM motor
Fixed duty EC Impeller motor
o Press hidden key to accept motor control function/type and exit
o Press key at any time to abort and exit.
Page 37
OM0280 Page 37 of 63
Rev 1 October/2021
7.5.3 Airflow Calibrations
Failure to calibrate airflow to the specified requirements may
result in unsafe conditions of performance (i.e. product and/or
personnel protection, noise and vibration)
The NU-540E airflow calibration consists of adjustments to balance the airflow within the cabinet. THIS WORK
SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN MEASURE THE AIRFLOW FROM THE FILTERS
WITH A SUITABLE VELOMETER. NuAire provides two adjustments to balance the airflow within the cabinet.
These are:
a. PWM signal adjust via calibration parameter menu
b. exhaust filter choke
The PWM signal or blower speed adjustment establishes the motor speed controls curve starting point of the
programmed internal reference performance curve while the choke adjusts or balances the exhaust airflow as well
as makes up for filter resistance tolerances. Since it has been NuAire's experience that the filters may not "load"
evenly, choke adjustments may be necessary for proper cabinet airflow balance and performance.
DC ECM motor PWM signal DC voltage should also be monitored and recorded upon final calibration. The DC
voltage may be measured using a digital voltmeter. The two test points to measure DC ECM motor voltage are
located on the DC motor connector on the main control board.
The cabinet is considered to be certifiable if the following airflow measurements are present:
a. Downflow average: 60 LFPM 5 LFPM (.30 m/s .025 m/s).
b. Inflow average: 105 LFPM 5 LFPM (.53 m/s .025 m/s) using the direct inflow measurement
method or constricted 3 inch (76mm) high access opening measurement method.
Note: It is recommended to perform the airflow calibration process at least annually to optimize both the
airflow control and PresurFlow™ monitor systems.
CAUTION
!
Page 38
OM0280 Page 38 of 63
Rev 1 October/2021
7.5.3.1 Downflow Calibration
Step 1: Place a velometer in the cabinet workzone on the horizontal plane 4 inches (102mm) above the
bottom edge of the viewing window. Spot check several points on the recommended downflow
velocity test grid in table 7.0
Step 2: If necessary, enter active blower speed adjustment.
• Press and hold key, then press hidden – blower - ↑ keys sequentially.
LED indicator above blower (green) key will blink fast.
Step 3: Press ↑ or ↓ keys to adjust blower speed.
• LED segments will indicate blower speed percentage and active blower speed adjustment
o Right end red LED indicates active blower speed adjust
The red LED will blink as soon as any adjustments are made and will continue to blink as the
motor rpm settles. Once the red LED stops blinking, the motor will run steady state at the
new percentage.
Note: The red LED must be non-blinking to save or exit
o Green LED’s indicate percentage on of scale (0-100%)
o Yellow LED’s indicate minimum (left/maximum (right) blower speed has been achieved
Note: At any time during the process
• Press hidden key to accept and enter the blower speed calibration point (confirmed with 3 audible
beeps).
(If the blower speed calibration point was not successfully entered with the 3 audible beeps. The
calibration process must then be repeated for successful entry of blower speed calibration point.)
• Press key to abort and exit
Step 4: Proceed to inflow calibration leaving in active blower speed adjustment
7.5.3.2 Inflow Calibration
Step 1: Measure the inflow velocity using the recommended procedure found in Table 7.0. If necessary,
adjust the exhaust filter choke, located under the front decorative panel, to achieve the correct
average inflow velocity within the stated range of 105 ± 5 LFPM (.53 ± .025 m/s).
• Less than 100 LFPM (.51 m/s);
First open the choke plate or make sure it is open.
If this is insufficient, then increase blower speed.
• Greater than 110LFPM (.56 m/s)
First decrease blower speed to achieve ½ the exhaust excess, then close the choke plate to
achieve the balance. In this fashion, the downflow should remain nearly constant (i.e. what
the reduced speed took away, the choke plate restores).
Note: The choke plate adjustment requires a standard blade screwdriver.
To adjust, loosen the liquid-tight fitting around the choke adjustment shaft.
While monitoring the exhaust flow to check position, turning the choke adjustment shaft
clockwise will open the choke while turning counter clockwise closed the choke.
Step 2: Once exhaust adjustment is complete, return to the downflow calibration process and measure
average downflow velocity. If the downflow average remains within the correct range, the
calibration is complete. Press hidden key to accept the blower speed calibration point. If not,
readjust as necessary to obtain the correct calibration range. Once entire cabinet has been
balanced, tighten liquid-tight fastener around choke adjustment shaft.
Note: Upon exit from the active blower speed adjustment, the PresurFlow™ will be calibrated to the
center green LED indicator. High/low alarm limits will be based on the default values established
from the calibration point.
Page 39
OM0280 Page 39 of 63
Rev 1 October/2021
7.5.3.3 PresurFlow™ Alarm Set Points
The PresurFlow™ alarm setpoints are preset based on the calibration setpoint. Once the calibration setpoint is
entered, based on a nominal inflow velocity of 105fpm (.53mls) the associated pressure sensor value is
entered as the nominal pressure value High and low alarm setpoints are factory verified and set if needed at
120fpm (.61mls) and 95fpm (.48mls).
However, if specific use alarm setpoints or re-entry of recommended alarm setpoints is desired during the
calibration process, the alarm setpoints may be adjusted by performing the following:
Low Alarm Setpoint
• Press and hold the hidden and ↓ key for 3 seconds.
(The left red LED will blink and the green LED’s indicate blower speed)
• Press ↑ or ↓ keys to adjust blower speed to the desired airflow velocity low alarm setpoint value.
• Left end red LED indicates active low limit blower speed adjust
The red LED will blink as soon as any adjustments are made and will continue to blink as the motor
rpm settles. Once the red LED stops blinking, the motor will run steady state ate the new percentage.
If the low alarm setpoint value is not within an acceptable range, the left end red LED will blink at a
very fast rate.
Note: The red LED must be non-blinking to save or exit.
Note: At any time during the process
• Press hidden key to accept low alarm setpoint value
• Press and hold outlet key for three seconds to remove any previous offsets
• Press key to abort and exit
• Upon exiting, the blower will go back to actual airflows.
High Alarm Setpoint
• Press and hold the hidden and ↑ key for 3 seconds.
(The right red LED will blink and the green LED’s indicate blower speed)
• Press ↑ or ↓ keys to adjust blower speed to the desired airflow velocity high alarm setpoint value.
• Red end Red LED indicates active high limit blower speed adjust.
The red LED will blink as soon as any adjustments are made and will continue to blink as the motor
rpm settles. Once the red LED stops blinking, the motor will run steady state at the new percentage.
If the high alarm setpoint value is not within an acceptable range, the right end red LED will blink at a
very fast rate.
Note: The red LED must be non-blinking to save or exit.
Note: At any time during the process
• Press hidden key to accept high alarm setpoint value
• Press and hold outlet key for three seconds to remove any previous offsets
• Press key to abort and exit
• Upon exiting, the blower will go back to actual airflows.
Note: Specific use alarm setpoints or the offset pressure value from the nominal calibration point will
be maintained with a new nominal calibration value. It is not necessary to re-enter the alarm
setpoints after a nominal calibration.
Page 40
OM0280 Page 40 of 63
Rev 1 October/2021
7.5.3.4 PresurFlow Alarm Verification
The PresurFlow Alarm setpoints are based on the calibration setpoint. Once the calibration setpoint is
entered, the Alarm setpoint offset pressure values will align from the calibration pressure value. The high or
low alarm setpoint can be verified by measuring inflow volume/velocity while adjusting blower up or down
within the Alarm Verification menu.
• Press and hold key, then press ↑ ↓ ↑ sequentially releasing the key after the 3 key
sequence.
• Note: If blower was off while entering into the Alarm Verification Menu, the low alarm limit will
immediately activate. Turn on blower; once airflow is above the low alarm limit, the alarm will turn
off.
• LED segments will indicate blower speed percentage.
• Press ↑ or ↓ key to raise or lower blower speed. Alarm is active so yellow and red LED’s will activate
if pressure reaches the low or high alarm limit.
• Press to exit (blower should turn off and not go through normal start up procedure).
7.5.3.5 Nite Care Calibration (See Section 8.2.2 to Activate the Nite Care Function)
The Nite Care mode is defaulted to operate the blower at approximately 600 rpm or a 14% duty cycle.
However, if desired the Nite Care blower speed can be adjusted higher or lower by performing the following:
• Press and hold key, then press hidden – blower - ↓ keys sequentially.
LED indicator above blower (green) key will blink fast.
Press ↑ or ↓ keys to adjust blower speed.
• LED segments will indicate Nite Care blower speed percentage and active blower speed adjustment
o Left end red LED indicates active blower speed adjust
The red LED will blink as soon as any adjustments are made and will continue to blink as the motor
rpm settles. Once the red LED stops blinking, the motor will run steady state at the new percentage.
Note: The red LED must be non-blinking to save or exit
o Green LED’s indicate percentage on of scale (0-100%)
o Yellow LED’s indicate minimum (left/maximum (right) blower speed has been achieved
Note: At any time during the process
• Press hidden key to accept Nite Care blower speed calibration point
• Press key to abort and exit
Page 41
OM0280 Page 41 of 63
Rev 1 October/2021
Table 7.0
Recommended Measurement Methods for Cabinet Downflow & Inflow
A. Downflow Measurement
a. Recommended Instruments: TSI 8355 Thermo anemometer
b. Procedure:
Supply filter efflux is measured on a grid, in a horizontal plane 4 inches (102mm) above the bottom edge of the
window. No reading should be taken closer than 6 inches (152mm) from the inside perimeter.
c. Test Data - Inches (mm):
Model Size
Window Access Height
300E
10
6.25
(159)
13.542
(344)
20.834
(529)
28.125
(714)
400E
10
7.0
(178)
12.396
(315)
17.792
(452)
23.188
(589)
28.584
(726)
33.980
(863)
39.375
(1000)
500E
10
7.25
(184)
13.518
(343)
19.786
(503)
26.054
(662)
32.322
(821)
38.590
(980)
44.858
(1139)
51.125
(1229)
600E
10
7.5
(191)
15.410
(391)
23.320
(592)
31.230
(793)
39.140
(994)
47.050
(1195)
54.960
(1396)
62.870
(1597)
6.25
(159)
7.0
(178)
7.250
(184)
7.5
(191)
12.5
(318)
12.5
(318)
12.5
(318)
12.5
(318)
18.750
(476)
18.0
(457)
17.750
(451)
17.5
(445)
Number of Readings:
Average Velocity ft./min.(m/s)
d. Acceptance Criteria:
1. Average downflow velocity = 55 to 65 fpm (.28 to .33 m/s)
2. Individual readings must be within + 20% or + 16fpm (+ 0.08m/s) whichever is greater (factory test)
or + 25% or + 16fpm (+ 0.08m/s) whichever is greater (field test) from the average downflow velocity.
B. Inflow Measurement
a. Recommended Instrument: Shortridge Flowhood ADM-870 or TSI 8355 Thermo anemometer.
b. Primary Procedure:
The primary procedure to determine inflow velocity uses a Direct Inflow Measurement (DIM) Instrument (i.e.
Shortridge flowhood).
The DIM Instrument can be used directly on the cabinet with NO CORRECTION FACTORS REQUIRED if operated in
the local density default mode.
NSF has tested the cabinet and established listed air velocities expressed in local density.
The DIM Instrument should also be duct taped to the cabinet to prevent any sneak air paths from occurring.
The DIM Instrument will read inflow volume (i.e. CFM).
Use the window access opening area to calculate inflow velocity.
Page 42
OM0280 Page 42 of 63
Rev 1 October/2021
Alternate Procedure:
The alternative procedure to determine inflow velocity uses a Thermo anemometer in a constricted window access opening
of 3 inches (76mm) with the armrest removed. Inflow air velocity is measured in the center of the constricted opening
1-1/2 inches (38mm) blow the top of the work access opening on the following specified grid.
Use the correction factor table to calculate the inflow velocity.
c. Test Data - Inches (mm):
1. Dim Measurement
Inflow Volume ft.3/min.(m3/s)
Access Opening ft.2(m2)
Inflow Velocity fpm(mps)
2. Constricted 3 inch (76mm) high access opening measurement - Inches (mm):
300E
4
8.396
12.792
17.188
21.584
25.980
30.375 (102)
(213)
(325)
(437)
(548)
(660)
(771)
400E
4
8.264
12.528
16.792
21.056
25.320
29.584
33.848
38.112
42.375
(102)
(210)
(318)
(426)
(535)
(643)
(751)
(860)
(968)
(1076)
500E
4
8.198
12.396
16.594
20.792
24.990
29.188
33.386
37.584
41.782
45.980
50.178
54.375
(102)
(208)
(315)
(421)
(528)
(635)
(741)
(848)
(955)
(1061)
(1168)
(1274)
(1381)
600E
4
8.158
12.316
16.474
20.632
24.790
28.948
33.106
37.264
41.422
45.580
49.738
53.896
58.054
62.212
66.375
(102)
(207)
(313)
(418)
(524)
(630)
(735)
(841)
(946)
(1052)
(1158)
(1263)
(1369)
(1475)
(1580)
(1686)
Number of Readings:
Average Velocity of Constricted Area fpm(mps)
1. Average Velocity of Constricted Area
fpm (m/s)
2. X Constricted Access Area
ft2 (m2)
3. = Constricted Area Volume
CFM(m3/s)
4.
Constricted Area Volume
CFM(m3/s)
5. 10”(254) Access Window Area
ft2(m2)
6. = Average Velocity of 10”(254) Access Window Area
fpm (m/s)
7.
Average Velocity of 10”(254) Access Window Area
fpm (m/s)
8. X Correction Factor for Window Height
9. = Average Inflow Velocity
fpm (m/s)
d. Acceptance Criteria: Access Opening Inflow Velocity = 100 to 110 fpm (.51 to .56 m/s)
Areas/Correction Factors for Calculations
Cabinet
Size
3" (76mm) Constricted
Window Access Area
10" (254mm)
Window Access
Opening
Correction Factor for
10" (254mm) Window
300E
.72
(.067)
2.39
(.222)
1.01
400E
.97
(.090)
3.22
(.299)
1.0
500E
1.22
(.113)
4.05
(.376)
1.0
600E
1.47
(.137)
4.89
(.454)
1.02
Page 43
OM0280 Page 43 of 63
Rev 1 October/2021
7.6 HEPA Filter Leak Test
In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly accessible by the measuring
instrument. The challenge material (i.e. PAO) should be supplied in the rear center of the workzone over the intake slots.
The upstream challenge port being common for both filters is located under the work surface with a red cap.
7.6.1 Supply Filter
The diffuser plate, placed below the HEPA to protect the filter during normal usage, may be removed as follows:
The diffuser is secured to the cabinet shell by #1/4-20 acorn nuts located immediately behind the front viewing
window. After removing the fasteners, drop the front of the diffuser plate several inches and pull forward gently.
Note that the diffuser is purposely a tight fit - it is held to the back wall of the cabinet interior by a light push - fit with
projecting studs. To avoid the window high alarm during the filter integrity check, it is desirable to enter the Service
menu and turn on the blower.
Air currents in the room can sometimes contribute to exhaust entrainment on top of the cabinet. This can lead to
false leak readings. In order to reduce the probability of false leak readings, it is recommended to cover half of the
exhaust HEPA filter while scanning the uncovered section of the exhaust HEPA filter.
7.6.2 Exhaust Filter
The exhaust filter is typically more difficult to check since protective grills, charcoal filters, or exhaust transitions
could cover the filter. Access panels are usually provided and should be removed. If an air gap exhaust transition is
provided, the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from
migrating into the exhaust efflux. All exhaust blowers/fans should be turned off during the check.
NOTE: To avoid the window high alarm during the filter integrity check, it is desirable to enter into the
Blower speed adjustment menu and turn the blower on. To accomplish this, perform the
following:
• Press and hold key,
then press ENT ↑ and ↓ keys sequentially releasing the key after the 3 key sequence.
LED segments will indicate blower speed percentage. However no adjustment is needed, do not adjust!
• Once filter integrity test is completed,
• Press key to abort and exit.
Note: If the upstream challenge port is deemed contaminated and not accessible, use both downflow
and exhaust volume for determining challenge concentrations. Use following area information
below with average downflow velocity and spot-check exhaust velocities as measured to
determine volume (CFM) (CMH).
Model
Size
*Supply Area
(ft2)(m2)
Exhaust Area
(ft2)(m2)
Model
Size
*Supply Area
(ft2)(m2)
Exhaust Area
(ft2)(m2)
300E
6.33 (.588)
1.61 (.150)
500E
10.74 (.998)
4.17 (.387)
400E
8.53 (.792)
3.06 (.284)
600E
12.95 (1.203)
5.00 (.465)
* Measured 4 inches above the bottom edge of the window.
Laskin Nozzle Concentration Formula
# Nozzles x 135 CFM x 100 ug/L Challenge
Downflow (CFM) + Exhaust (CFM) Concentration (ug/L)
# Nozzles x 229 CMH x 100 ug/L Challenge
Downflow (CMH) + Exhaust (CMH) Concentration (ug/L)
=
=
Page 44
OM0280 Page 44 of 63
Rev 1 October/2021
7.7 Airflow Smoke Pattern Test
The airflow smoke pattern test is performed using a smoke source (i.e. smoke tubes) in and around the cabinet workzone
and access opening to determine a visual representation of the cabinet’s containment performance. To perform the test,
the smoke source should be passed through the following areas:
A smoke source shall be passed:
1. From one end of the cabinet to the other, along the center line of the work surface, at a height of 4 inches
(102mm) above the top of the access opening.
2. From one end of the cabinet to the other, 1 inch (25mm) just inside the view screen, at a height 6 inches
(152mm) above the top of the access opening.
3. Along the edges of the entire perimeter of the work opening approximately 1.5 inches (38mm) outside the
cabinet, with particular attention paid to corners and vertical edges.
4. 2 inches (51mm) from the sides up inside of the window at the side channel seals, and along inside of the
cabinet along the top of the work area or immediately below the wiper gasket.
The criteria used to evaluate the smoke patterns is the following:
1. The smoke inside the cabinet shall show smooth downward flow with no dead spots or reflux.
2. No smoke shall escape from inside the cabinet.
3. No smoke refluxes out of the cabinet once drawn in, nor does smoke billow over the worksurface or penetrate
onto it.
4. No smoke shall escape from the cabinet.
7.8 Site Installation Assessment Tests
These tests are performed to verify the sash position, airflow or pressure setpoint where an audible and/or visual alarm will
activate to signify unfavorable operating conditions within the Biosafety cabinet and/or the remote exhaust blower, and
canopy connection performance.
7.8.1 Sash Alarm
Step 1: With sash alarm switch enabled, raise the sliding sash 1” (2.5cm) above the manufacturer’s
designated sash height for normal operation. Verify that the audible/visual alarm activates/sounds.
Step 2: Return the sash to its normal operating height.
Note, if necessary, adjustment of the sash alarm switches can be accomplished by:
Behind the right side faring there is a cover panel that needs to be removed.
Then you can access the microswitches and adjust the trip point. Adjustment
is not just vertical, but as you see in the following picture, we have allowed tolerance for rotational
adjustment too. Sometimes how the window switch roller is positioned
will affect its activation point.
Use a nut driver to loosen the switch plate, adjust and re-tighten
Page 45
OM0280 Page 45 of 63
Rev 1 October/2021
7.8.2 Airflow or Pressure Alarm (when installed)
Step 1: Measure and record the speed control operating voltage at the speed control test points using a
voltmeter.
Step 2: Using the primary or secondary inflow test method, lower the speed control voltage to reduce the
inflow by 20% from the certified testing value.
Step 3: Verify that the alarm activates when the inflow is dropped to this point.
Step 4: Adjust alarm setpoint as necessary as instructed by the alarm manufacturer procedures.
Step 5: Return the speed control to its certified operating voltage as measured by the voltmeter.
7.8.3 Exhaust System Performance
Canopy Exhaust Transitions
Step 1: Introduce a visible medium source into the canopy air intake(s) while slowly reducing the exhaust
volume until there is a loss of capture of the visible medium into the canopy air intake(s).
The audible and visual canopy alarms shall respond within 15 seconds, and the cabinet fan(s) will
continue to operate.
Step 2: Return exhaust volume to original value.
NOTE: Positive pressure ductwork conditions are strongly discouraged. Positive pressure ductwork conditions
should be reviewed and evaluated by a cognizant Safety Officer of Industrial Hygienist as part of their
Laboratory Ventilation Management Program and Chemical Hygiene Plan.
Page 46
OM0280 Page 46 of 63
Rev 1 October/2021
7.9 Cleanliness Classification Test for Pharmacy Application
If this cabinet is going to be used within pharmacy, per USP7971, the cabinet must be tested to assure compliance to ISO
14644-1:2015, Cleanrooms and Associated Controlled Environments, Part 1: Classification of Air Cleanliness2. The
cleanliness classification test is performed using a particle counter to measure particle counts within the cabinet workzone.
Turn on cabinet and let warm up for several minutes. Turn on particle counter and flush out sample tubing line to remove
latent particles. Set the particle counter to measure 0.5 micron or larger particles at the appropriate measuring rate.
“Operational Particle Count Test3”
Position the particle counter isokinetic probe at a point 6 inches (152mm) upstream of the aseptic manipulation area (hand
convergence point) and mounted so as not to interfere with the operator’s hand movement. The pharmacy operator will
simulate IV manipulation during the particle count test using non-hazardous materials. A minimum of three (3) 1-minute
particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations.
“At Rest Particle Count Test”
Take 5 test points in 1-minute intervals on a grid, in a horizontal plane as measured approximately 6 inches (152mm) above
the worksurface. The grid location is designed as the workzone center point and each corner measured 6 inches (152mm)
from the inside perimeter.
Record the 5 particle count values for each of the test points over the 1-minute sample time.
All final count particle concentrations and calculated 95% upper confidence limit shall not exceed 3520 particles per cubic
meter (ppcm) or 100 particles per cubic feet (ppcf).
1
USP28-NF23: United Stated Pharmacopeial Convention, Inc., 12601 Twinbrook Parkway, Rockville, MD
20852, USA, www.usp.org.
2
ISO 14644-1:2015 Cleanrooms and Associated Controlled Environments-Classification of Air Cleanliness, International Organization for Standardization,
Case Postale 56, CH-1211 Geneve 20, Switzerland
3
CAG-002-2006: CETA Compounding Isolator Testing Guide, Controlled Environment Testing Association, 1500 Sunday Drive, Suite 102, Raleigh, NC
27607, USA, www.cetainternational.org
Page 47
OM0280 Page 47 of 63
Rev 1 October/2021
7.10 Main Control Board Description and Replacement
To access the main control board for fuse or board replacement, remove screws at each upper side of the control center
and allow the control center to rotate down, resting on the safety straps. Now the main control board is exposed for
service.
7.10.1 Main Control Board Replacement
The main control board consists of one Printed Circuit Board (PCB) assembly.
The PCB contains the power supply, configuration switch, sensor inputs/outputs and control inputs/outputs
components and display.
7.10.2 Main Control Board Fuse Replacement
Disconnect electrical power from cabinet before fuse replacement.
All AC circuits are fuse protected and when replacement is necessary, USE ONLY FUSES OF SAME TYPE AND RATING
FOR PROTECTION AGAINST RISK OF FIRE.
DESCRIPTION:
BLOWER FUSE
OUTLET FUSE
ACCESSORY OUTPUT FUSE
LIGHT FUSES
FUSE TYPE:
TIME-LAG
TIME-LAG
TIME-LAG
TIME-LAG
FUSE SIZE:
1/4 X 1-1/4 INCH
5 X 20MM
5 X 20MM
5 X 20MM
NU-540-300E
6.25 AMPS
3 AMPS
2 AMPS
1 AMP (2)
NU-540-400E
6.25 AMPS
3 AMPS
2 AMPS
1 AMP (2)
NU-540-500E
8 AMPS
3 AMPS
2 AMPS
1 AMP (2)
NU-540-600E
8 AMPS
3 AMPS
2 AMPS
1 AMP (2)
7.10.3 Main Control Board Replacement
Note: All setup and calibration data will be lost, the memory reinitialized to the default values and all control
functions reset to an initial cabinet power condition.
If possible, before the main control board replacement, it would be preferred to know the operational
parameters of the cabinet, (i.e. blower speed/PWM signal DC voltage setpoints if modified and airflow data
from previous certification).
The main control board is fastened to the control center with (6) 6-32 studs/nuts. All electrical connections are
made with removable terminals and/or Faston connectors except for the motor/blower connector which uses a
screw terminal. Remove all electrical connections and fasteners and then remove the main control board from the
control center.
Install new main control board by reattaching all electrical connections and fasteners. Once installed, rotate
control center to normal position and fasten in place.
Reconnect power to cabinet.
CAUTION
!
CAUTION
!
Page 48
OM0280 Page 48 of 63
Rev 1 October/2021
7.10.4 Aeromax™ Control System Reset
The Aeromax™ control board has a reset function available for Qualified Service Personnel.
Master Reset-Resets all calibration, cabinet size, motor type/function and option settings back to their default
condition. It is recommended to perform a master reset upon installation of replacement control board or if there
seems to be intermittent functional abnormalities.
Perform the following sequence to accomplish a master reset:
1. Turn off all functions i.e. blower, lights, etc.
2. Press and hold alarm silence key for 10 seconds until alarm LED flashes, then release.
3. Press hidden key within 5 seconds of the alarm LED flashing. There will be a (3) beep confirmation that
the reset has occurred.
4. Turn off cabinet (either unplug cabinet or use power switch on left side within control panel) then turn
back on to reinitialize the Aeromax™ control board. Just as in the initial power up, the RED alarm LED will
blink to indicate power up status.
5. Press key to clear the power on reset message.
6. Enter into service parameters and input configuration for calibration and option settings as required.
8.0 Error Messages, Troubleshooting, Option-Diagnostics and Airflow Sensor Performance Verification
Audible alarms and error messages occur for a variety of reasons. Whenever an alarm condition has been present for a
period of at least 10 seconds, the audible alarm/error message will be presented and stay on until the error is cleared. The
audible alarm will be on for 30 seconds upon initial alarm condition, then once every ten seconds. When presented with an
error message, please perform the following:
Step 1: NOTE ALL ERROR MESSAGES.
Error message will appear on the control panel with red LED’s.
Step 2: VERIFY ERROR MESSAGES.
Error messages can be verified by clearing the error function by either turning the blower or the cabinet on
and off.
Step 3: MONITOR RE-OCCURRENCE OF ERROR MESSAGES.
If re-occurrence of the error message is immediate or daily, use the following guide to correct the situation.
Page 49
OM0280 Page 49 of 63
Rev 1 October/2021
8.1 Error Message Troubleshooting Guide
Error Message
Error Description
Correction
Window Alarm
(Window High)
Sliding window is above standard working height
or micro switch is not operating properly.
Verify standard working height and window micro
switch operation.
Window Alarm
(Window Low)
Window Closed
Sliding window is below standard working height
or micro switch is not operating properly.
Indicates the window is in the full down position
Verify standard working height and window micro
switch operation.
Cabinet LED lights
won’t Turn on
Blue LED above light key indicates the lamp should
be on.
Check light fuse on main control board.
Check LED lamps.
Check voltage coming out of main control board to
light ballasts.
Check light starters, if present. Check ballast.
Cabinet blower won't
turn on.
Green LED above blower key indicates the blower
should be on.
Airflow Alarm.
Check blower fuse on main control board.
Check AC voltage coming out of main control board.
Check wiring to blower.
Check blower motor.
Check DC motor PWM signal on main control board.
Red alarm LED blinks
Indicates a power interruption has occurred.
Press any key to clear.
Cabinet outlets won't
turn on.
Blue LED above outlet key indicates the outlets
should be on.
Check outlet fuse located on main control board.
Check voltage coming out of main control board.
Cabinet ultraviolet
light
won't turn on.
Yellow LED above UV light key indicates the UV
lamp should be on.
Check sliding window position- should be fully
closed.
Check blower/lights fuse on main control board.
Check voltage coming out of the main control board
to ultraviolet light ballast.
Check ballast.
Blower or light fuse
continues to blow
after replacement.
N/A
Check for short on output of fuse.
Isolate output of fuse by disconnecting control
center
connectors, light circuit, AC or DC blower circuit,
etc. to isolate the short.
UV LED blinks fast and
red LED alarm
Indicates that the UV light needs replacement
Replace UV light and clear UV run time clock.
Blower green LED
blinks fast and
PresurFlow™ 3 green
LED’s indicators on
Indicates that the Nite Care is activated,
preventing the usage of the cabinet.
N/A
PresurFlow™ left red
LED indicator on and
red LED alarm
PresurFlow™ reading low flow (pressure)
Check airflow values.
Check blower function.
Recalibrate PresurFlow™ system.
PresurFlow™ right red
LED indicator on and
red LED alarm
PresurFlow™ reading high flow (pressure)
Check airflow values.
Recalibrate PresurFlow™ system.
All PresurFlow™ LED’s
blink
Message acknowledges new firmware was
loaded into microprocessor
N/A
Blower red LED blinks
and red LED alarm
Indicates that the motor rpm signal has been
interrupted
Check connectors and wires from main control
board
to the motor
Replace motor if required
Page 50
OM0280 Page 50 of 63
Rev 1 October/2021
8.2 Option Parameters
The option parameter menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters per the
menu as described below.
8.2.1 Sync Function with Active Blower
To access the option parameter menu, perform the following:
• Press and hold key, then press hidden - Blower – LED light keys sequentially.
Red LED indicator above the blower key will blink fast
The PresurFlow™ blinking green LED segments will indicate seven optional parameters as shown and described
below. The UV Light key (move lefts) and outlet key (move right) allows selection of the option parameter desired.
Once the desired option parameter is indicated, press ↑ or ↓ key to turn on or off. A slow blinking green LED
indicator means off and a fast blinking green LED indicator means on. Multiple option changes can be selected.
• Pressing the hidden key will accept all changes and exit
• Pressing the key will abort the process and exit
Sync Fan Relay with Active Blower -
Normally the fan relay will activate when the blower switch is pressed. Blower can
either be actively running or pending. If the fan relay sync is active the blower must
be actively running for the relay to change state.
Sync Accessary Outlet with Active Blower –
Normally the accessary outlet is on all the time. If the accessary outlet sync is active,
the blower must be actively running for the accessary outlet to turn on.
Sync Outlet Power with Active Blower –
Normally the outlet power is turned on via the outlet key. If the outlet power sync is
active, the outlet power will turn on and off with the blower or may be turned on and
off independently if the blower is active.
Sync LED Light with Active Blower –
Normally the LED light is turned on via the LED light key. If the LED light sync is active,
the LED light will turn on and off with the blower or may be turned on and off independently if the blower is active.
Sync 15 Volt DC output with active blower normally the 15 Volt DC output located on
the control board is on when power is applied to the system. If the 15 Volt DC output
sync is active, the blower must be actively running for the 15 Volt DC output to turn
on.
8.2.2 Blower/Airflow Options
To access the option parameter menu, perform the following:
• Press and hold key, then press hidden - ↑ and ↓keys sequentially.
Red LED indicator above the blower key will blink fast
The PresurFlow™ blinking green LED segments will indicate seven optional parameters as shown and described
below.
The UV Light key (moves left) and outlet key (moves right) allows selection of the option parameter desired.
Once the desired option parameter is indicated, press ↑ or ↓ key to turn on or off. A slow blinking green LED
indicator means off and a fast blinking green LED indicator means on. Multiple option changes can be selected.
• Pressing the hidden key will accept all changes and exit
• Pressing the key will abort the process and exit
Page 51
OM0280 Page 51 of 63
Rev 1 October/2021
Require Password –
Normally it is not required to use a password (i.e. 3 key press sequence of the blower
and hidden key). If the option is turned on, it would be required to use the correct
password to turn on the blower. The default password once turned on is blowerhidden-blower keys in sequence. The password can be changed in the blower
password option menu.
Allow UV light anytime –
Normally the UV light is interlocked with the window being in the closed position.
For service purposes only, if the function is active, the UV light may be turned on at
any window height.
Note: In addition to the Allow UV light anytime system function, there is a double redundant UV light
window interlock relay. To override the UV light window interlock relay, the relay itself must
also be shorted. (See electrical schematic for reference).
Manual Blower Restart –
Normally when the blower is actively running and a power interruption occurs.
The blower will automatically come back on when power is restored. If this function
is turned off, the blower will not automatically come back after a power interruption,
but would require the user to press the blower key to restart the blower.
Nite Care –
Normally the Nite Care function is turned off. If selected and turned on, once the
blower is actively running. Upon closure, the blower will continue to run at a
calibrated lower speed level to keep the workzone interior sterile. The PresurFlow™
will indicate(s) blinking green LEDs along with green LED above blower key. The LED
light will turn off (blue LED above light key will blink if window closure turned it off)
and become inoperable; however the UV light can be used.
Disable PresurFlow™ -
If this function is active, the digital pressure sensor and alarm function are turned off.
Temporary Nite Care -
When this option parameter is turned on and the other requirements below are met,
the blower key (when held for 5 seconds) will toggle blower between normal and Nite
Care blower speed. The Nite Care blower will time out, based on the Auto Timer
duration for Nite Care without a blower key press and the blower speed will revert
back to normal. Closing the window will automatically terminate.
In addition to turning this option on the following requirements must also be met
• The Nite Care must be selected
• Password must be selected
• Window is at normal height and blower is running
• Nite Care blower auto timer must be set for a minimum of 15 minutes.
Disable audible alarms / audible key feedback Normally audible alarms from the
PresurFlow™ monitor and audible key feedback are present to provide an audible
sound to the user. If this function is active, all audible sound will be silenced when the
control system is in normal run mode. Audible sound will still occur in any
service/calibration menu function.
Page 52
OM0280 Page 52 of 63
Rev 1 October/2021
9.0 Remote Contacts
The NU-540E has several contact closures for remote sensing of various functions.
9.1 Fan Relay
The fan relay contacts are normally open and closed contact closure outputs that are activated whenever the blower key is
pressed and the blower key LED indicator is on or blinking. Contact ratings are 250 VAC maximum at 2 Amps.
9.2 Alarm Relay
The alarm relay contacts are normally open and closed contact closure outputs which are activated whenever an airflow
alarm condition occurs. Contact ratings are 250 VAC maximum at 2 Amps.
9.3 15VDC Output
The 15VDC (100mA) output is generated if the blower is actively running.
Page 53
OM0280 Page 53 of 63
Rev 1 October/2021
10.0 Optional Equipment
10.1 Ultraviolet Light
Ultraviolet light will injure your eyes. Avoid direct viewing at all times.
Personnel should not be present when ultraviolet lamp is on
10.1.1 Overview
The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms in the air
or on directly exposed surfaces. Approximately 95% of the ultraviolet radiations from germicidal tubes are in the
253.7 nanometer region. This is a region in the ultraviolet spectrum which is near the peak of germicidal
effectiveness. The exposure necessary to kill bacteria is the product of time and intensity. High intensities for a
short period of time, or low intensities for a longer period are fundamentally equal in lethal dosage on bacteria
(disregarding the life cycle of bacteria). The intensity of light falling on a given area is governed by the inverse law;
that is the killing intensity decreases as the distance increases from the tube.
The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per centimeter (for
a new tube) falling on a horizontal plane defined by the bottom of the work surface. The minimum requirement
per paragraph 5.12 of NSF Standard 49 is 40 microwatts per square centimeter (ref. NSF Std. #49, June, 1976).
Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be closed while
the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.
10.1.2 Operation
The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch
located on the front panel. The sliding window is interlocked to the ultraviolet light so, when the sliding window is
raised, the ultraviolet light will turn off. If operational time duration is known, the timer can be used in
conjunction with the ultraviolet light to time out the ultraviolet light operation. This can be accomplished by first
turning on the ultraviolet light. Then, set the timer to the desired length of ultraviolet light operation time. Upon
timer expiration, the ultraviolet light will turn off.
10.1.3 Precaution
The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening of the skin,
if of sufficiently high intensity, or if exposure covers a prolonged period of time. For this reason, one should avoid
direct eye and skin exposure to ultraviolet light. If exposure cannot be avoided, it is necessary for personnel to
wear eye goggles or face shields, and long sleeve gowns with rubber gloves.
Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be closed while
the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.
CAUTION
!
Page 54
OM0280 Page 54 of 63
Rev 1 October/2021
10.1.4 Maintenance
The output of an ultraviolet light deteriorates with burning age. The useful life of the light is approximately 5000
hours under specific test conditions.
The control system monitors UV light usage hours and when the 5000 hour point is reached. The UV light LED
indicator will blink fast along with the red LED alarm indicating the UV light should be replaced. To reset the timer,
perform the following:
• Press and hold key the press UV light – UV light – UV light key sequentially and the reset will occur
removing the above alarm condition.
•
It is also possible to approximate the UV light usage by the following:
• Press and hold key and UV light key together for three seconds. The PresurFlow™ LED’s will then
indicate usage of approximately 450 hours per LED light from left to right.
Indicates approximately 450 hours used
Indicates approximately 900 Hours used
Indicates approximately 3600 hours used
Note: Before testing with lamp off, the light may be cleaned with a lint-free cloth dampened with alcohol
or ammonia and water.
Page 55
OM0280 Page 55 of 63
Rev 1 October/2021
Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation (e)
Mold Spores
Microwatt
seconds
per cm/2
Protozoa
Microwatt
seconds
per cm/2
Penicillium roqueforti
26,400
Paramecium
200,000(a)
Penicillium expansum
22,000
Penicillium digitatum
88,000
Nematode Eggs
40,000(b)
Aspergillus glaucus
88,000
Aspergillus flavus
99,000
Algae
22,000(c)
Aspergillus niger
330,000
Rhizopus nigricans
220,000
Virus
Mucor racemosus A
35,200
Baceriophage (E. Coli)
6,600
Mucor racemosus B
35,200
Tobacco Masaic
440,000
Oospora lactis
11,000
Influenze
3,400(d)
Yeasts
Saccharomyces
13,200
ellipsoideus
17,600
Saccharomyces cerevisiae
13,200
Brewers' yeast
6,600
Baker's yeast
8,800
Common yeast cake
13,200
Bacteria
Streptococcus lactis
8,800
Strep. hermolyticus (alpha type)
5,500
Staphylococcus aureus
6,600
Staphylococcus albus
5,720
Micrococcus sphaeroides
15,400
Sarcina lutea
26,400
Pseudomonas fluorescens
7,040
Escherichia coli
7,040
Proteus vulgaris
7,480
Serratia marcescens
6,160
Bacillus subtilis
11,000
Bacillus subtilis spores
22,000
Spirillum rubrum
6,160
References:
(a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy,
D. Van Nostrand o., New York, New York, pp 253
(b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162
(c) Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp., pp. 713-714
(d) Hollaender, A., Oliphant, J.W. (1944)
The inactivation effect of monochromatic ultraviolet. Radiation on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454
(e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation's" comes from Westinghouse brochure
entitled - "Westinghouse Sterilamp Germicidal Ultraviolet Tubes"
Page 56
OM0280 Page 56 of 63
Rev 1 October/2021
11.0 Electrical/Environmental Requirements
11.1 Electrical (Supply Voltage Fluctuations Not to Exceed +/- 10%)
*NU-540-300E 230 VAC, 50/60 Hz, 1 Phase, 8 Amps
*NU-540-400E 230 VAC, 50/60 Hz, 1 Phase, 10 Amps
*NU-540-500E 230 VAC, 50/60 Hz, 1 Phase, 11 Amps
*NU-540-600E 230 VAC, 50/60 Hz, 1 Phase, 11 Amps
11.2 Operational Performance (for indoor use only)
Environment Temperature Range: 60F-85F (15C - 30C)
Environment Humidity: Maximum relative humidity 80% for temperatures up to 31C decreasing linearly to
50% relative humidity at 40C.
Environment Altitude: 6562 Feet (2000M)
11.3 Light Exposure
Standard LED Lighting @ 150 ft. candles (1614 LUX) maximum intensity.
11.4 Installation Category: 2.0
Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to
withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example,
in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public mains
such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500 V
for a 230 V supply and 1500 V for a 120 V supply.
11.5 Pollution Degree: 2.0
Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2
assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity
caused by condensation.
11.6 Chemical Exposure
Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting. USE OF CHLORINATED
OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL. Equipment decontamination can be
accomplished by non-condensing gas or vapor paraformaldehyde, Hydrogen Peroxide or Chlorine Dioxide following
NSF/ANSI Annex G or EN 12459 Annex J.
11.7 EMC Performance (classified for light industrial)
Emissions: EN61326
Immunity: EN61326
Class A equipment is intended for use in an industrial environment.
In the documentation for the user, a statement shall be included drawing
attention to the fact that there may be potential difficulties in ensuring
electromagnetic compatibility in other environments, due to conducted
as well as radiated disturbances.
WARNING
!
Page 57
OM0280 Page 57 of 63
Rev 1 October/2021
12.0 Disposal and Recycle
Cabinets that are no longer in use and are ready for disposal contain reusable materials. ALL components with the
exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected.
Note: Follow all local, state and federal guidelines for disposal of HEPA filter solid waste.
Prior to any disassembly for disposal, the cabinet must be decontaminated.
Component
Material
Base Cabinet
Stainless Steel
Front Grill
Stainless Steel
Worksurface
Stainless Steel
Window Faring
Stainless Steel
Window Glides
HDPE
Window
Safety Glass
Window Frame
Stainless Steel
Front Service Panel
Painted Steel
Front Decorative Panel
Painted Steel
Control Center
Painted Steel
Supply Diffuser
Aluminum
Exhaust Filter
Aluminum
HEPA Filter Frames
Painted Steel
Hepex Bag
PVC
Blower Wheel and Housing
Steel
Motor
Various Steel
Printed Wiring Assembly
Lead Free Electronic
Wire
PVC Coated Copper
Ballasts
Various Steel, Electronic
Armrest
PVC
Connectors
Nylon
Hardware
Stainless Steel and Steel
Note: Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic.
BIOHAZARD
RECYCLE
LEAD FREE
CAUTION
!
Page 58
OM0280 Page 58 of 63
Rev 1 October/2021
Page 59
OM0280 Page 59 of 63
Rev 1 October/2021
Page 60
OM0280 Page 60 of 63
Rev 1 October/2021
Page 61
OM0280 Page 61 of 63
Rev 1 October/2021
Page 62
OM0280 Page 62 of 63
Rev 1 October/2021
Page 63
OM0280 Page 63 of 63
Rev 1 October/2021
Loading...