Nikon eclipse Ti-U/B, eclipse Ti-U, eclipse Ti-S, eclipse Ti-S/L100, Ti-U Instructions Manual

...

Download

Page 1

Inverted Microscope

Instructions

M449 E 07.9.NF.1

Page 2

Page 3

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of Nikon Inverted Microscope Eclipse Ti-U, Ti-U/B, Ti-S, and Ti-S/L100.

To ensure correct usage, read this manual carefully before operating the product.

• No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

• The contents of this manual are subject to change without notice.

• Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may

remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon representative.

• Some of the equipment described in this manual may not be included in the set you have purchased.

• If you intend to use any other equipment with this product, read the manual for that equipment too.

• When using TI-HUBC/B Hub Controller B with the microscope, also refer to the instructions manual

provided with the hub controller.

• If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Page 4

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

Warning and Caution Symbols in this Manual

Although this product is designed to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. Do not discard this manual and keep it handy for easy reference.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols.

Symbol Description

Warning

Disregarding instructions marked with this symbol may lead to serious injury or death.

Caution

Disregarding instructions marked with this symbol may lead to injury or property damage.

Symbols on the Product

The symbols on the product indicate the need for caution at all times during use. Before operating a part labeled with the following symbols, refer to the instruction manual and read the relevant instructions.

Symbol Meaning

CAUTION: HOT

This symbol can be found on the top of the dia pillar illuminator and on the 12V 100W lamphouse, and cautions the following:

• The lamp and the lamphouse will be extremely hot while and immediately after using the lamp.

• To avoid the risk of burns, do not touch the lamp and the lamphouse while or immediately after using the lamp.

• When replacing the lamp, wait for the lamp and the lamphouse to cool sufficiently.

Biohazard

This symbol can be found on the upper part of the microscope, and cautions the following:

• The product may become biohazardous if a specimen is spilled onto the product.

• To avoid exposure to biohazard, do not touch contaminated parts with your bare

hands.

• Decontaminate the contaminated parts according to the standard procedures for your facility.

Page 5

Safety Precautions

Warning

1. Intended application of the product

The product is intended mainly for microscopic observations and micromanipulations of living cells and tissues under diascopic or episcopic illumination. It is designed for the purposes of experimentations and observations at hospitals or laboratories in the fields of genetics, immunology, physiology, pharmacology, neurology, cellular biology, and molecular biology.

The product is classified as an in-vitro diagnostic medical device.

2. Do not disassemble.

Disassembling this product may result in electric shock or malfunction. Malfunctions and damage due to such mishandlings will not be warranted. Do not disassemble any part that is not indicated in this manual. If you experience problems with the product, contact Nikon.

3. Read the instructions thoroughly.

To ensure safety, thoroughly read this manual and the manuals for other equipment to be used with this product. In particular, be sure to follow the warnings and cautions at the beginning of the manuals.

4. Check the input voltage.

The dia illumination lamp uses one of two types of power supply devices.

• TI-PS100W Power Supply:

The TI-PS100W Power Supply device can be used with 100 to 240 VAC at 50/60 Hz, and can be used with most wall outlets in the world. Under normal use, you will not need to pay particular attention to the supplied power voltage.

• TE-PS30W Power Supply A or TE-PSE30 Power Supply A:

The input voltage ratings are indicated on the rear panel of the power supply device. Before connecting the power cord, check that the indicated input voltage matches the voltage of the wall outlet. If the indicated input voltage does not match your regional voltage supply, do not use the power supply device, and contact Nikon. Use of a power supply device with the inappropriate voltage rating may result in overheating or fire due to overcurrent, and may also cause damage the power supply device and connected devices.

5. Cautions on the power cord

Be sure to use specified power cord for the power supply device. Use of other power cords may result in malfunction, overheat, or fire.

• See Chapter 7, “Specifications” for the specified power cord.

• To prevent electric shock, turn off the POWER switch (press the “O” side of the POWER

switch) on the power supply device before connecting or disconnecting the power cord.

• Note that the power supply device is classified as Class I for electric shock protection. Be sure to connect it to a protective ground terminal.

Page 6

Safety Precautions

Warning

6. Check the combination of dia pillar illuminator, lamp, and power supply device.

The combination of the dia pillar illuminator and the power supply device is specified based on the lamp ratings (12V/100W or 6V/30W) and the power voltage. Use them in the correct combination according to the instructions on page 55. Use of the devices in a wrong combination may result in malfunction, overheat, or fire.

7. Cautions on heat from the light source

The lamp and the lamphouse become extremely hot when the lamp is turned on. Follow the cautions below to prevent burns and fire.

• To avoid burns, do not touch the lamp and the lamphouse while the lamp is on or for approximately thirty minutes after it has been turned off.

• To avoid the risk of fire, do not place fabric, paper, or highly flammable volatile materials (i.e. gasoline, petroleum benzine, paint thinner, and alcohol) near the lamphouse while the lamp is on or for about thirty minutes after it has been turned off.

• Do not block the air vents on the lamphouse. If the lamphouse becomes covered or items are placed on the lamphouse, heat dissipation may be hindered, causing the lamphouse to become abnormally hot.

• The bottom of the power supply device becomes hot during use. Do not cover the air vents on the side of the power supply device.

8. Cautions on lamp replacement

• When replacing the lamp, wait approximately thirty minutes after turning off the lamp, and

make sure that the lamp and the lamphouse have cooled sufficiently.

• To prevent electric shock and product damage, turn off the power switch (set the switch to the "O" side) and unplug the power cords from the wall outlets before replacing the lamp.

• After replacing the lamp, close and secure the lamphouse cover. Never use the product with the lamphouse cover left open.

• Do not break the used lamp. It should be disposed of as an industrial waste, according to the local regulations and rules.

9. Notes on handling hazardous specimens

The product is intended mainly for microscopic observations and micromanipulations of specimens such as living cells and tissues in a Petri dish. Note the following when handling the specimens:

• Before handling a specimen, check whether it is hazardous. Wear rubber gloves when handling hazardous (i.e. potentially infectious) specimens.

• Be careful not to spill the specimen. If a specimen is spilt onto the microscope, decontaminate according to the standard procedures for your facility.

Page 7

Safety Precautions

Warning

10. Notes on handling flammable solvents

The following flammable solvents are used with the product:

• Immersion oil (Nikon Immersion Oil for oil immersion objectives)

• Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

• Petroleum benzine (for wiping off the immersion oil)

• Medical alcohol (for disinfecting the microscope)

Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions provided by the manufacturer, and handle correctly and safely. Note the following precautions when using solvents with the product.

• Keep solvents away from the lamp, the lamphouse, the power supply device, and any other parts that may become hot.

• Keep solvents away from the product and its surroundings when turning on/off the power switch or plugging/unplugging the power cord.

• Be careful not to spill the solvents.

Page 8

Safety Precautions

Caution

1. Turn off the power during installation, assembly, connection/disconnection of cables, and maintenance.

To prevent electric shock and fire, be sure to turn off the POWER switch on the power supply device (press the “O” side of the POWER switch) and unplug the power cords before installing or assembling the product, connecting or disconnecting cables, replacing the lamp, or performing maintenance tasks such as cleaning of the lenses.

2. Do not wet the product or allow intrusion of foreign matters.

Do not wet or spill liquids onto the product, as it may result in malfunction, overheat, or electric shock. If water or other liquids are accidentally spilled onto the product, immediately turn off the POWER switch (press the “O” side of the POWER switch) and unplug the power cord from the wall outlet. Then, wipe off the liquid with a piece of dry cloth. Intrusion of foreign matters into the product may also result in malfunctions. If liquids or foreign matters enter the product, do not use the product, and contact Nikon.

3. Weak electromagnetic waves

The product emits weak electromagnetic waves. Do not install the product near precision electronic devices to avoid affecting their performance. If signal reception by a TV or radio is affected, move the TV or radio set away from the product.

4. Cautions on moving the product

• When carrying the product, hold the product firmly by the bottom front recess and the bottom

rear.

• When moving the product, do not hold by the focusing knobs, eyepiece tube, stage, or dia

pillar illuminator. The parts may become detached and cause the product to fall, and may also result in malfunctions and loss of precision.

5. Cautions on assembling the product

• Take care to avoid pinching your fingers and hands.

• Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will

degrade the microscope image. When assembling the product, be careful not to scratch or directly touch the optical components.

6. Cautions on the protruding rack of the rectangular stage

The stage rack of TI-SR Rectangular Mechanical Stage will protrude when the stage is operated. When operating the focus knobs or the nosepiece, be careful not to strike your hands against the rack. Contact with the edges of the rack may result in injury.

7. Disposal

To avoid biohazard risks, dispose of the product as contaminated equipment, according to the standard procedures for your facility.

Page 9

Safety Precautions

Notes on Handling the Product

1. Handle with care.

The product is a precision optical instrument. Handle the product with care and avoid physical shocks and vibrations. In particular, the precision of objectives may be lost by even weak physical shocks.

2. Installation location and storage location

The product is a precision optical instrument. Use or storage of the product under inappropriate conditions may result in malfunctions or loss of precision. The following conditions must be considered for the installation location and the storage location.

• Install the product in a location with a temperature of 0 to 40ºC and a relative humidity of 85% or less (no condensation). Store the product in a location with a temperature of -20 to 60ºC and a relative humidity of 90% or less (no condensation). Use or storage of the product in a hot or humid location may result in molding of or condensation on the lenses, loss of precision, and malfunctions.

• Install the product in a place with little dust and dirt. When storing the product, place a cover over the product to protect it from dust.

• Install the product in a place with little vibration.

• Install and store the product on a level and sturdy table or stage that can bear the weight of the

product. Install the product in a location with minimal exposure to hazards in the event of earthquakes and other potential disasters. If necessary, secure the product to the working desk or other heavy and stable items with a strong rope or other means, so as to prevent it from falling.

• Avoid placing the product in direct sunlight or immediately under the room lights. The image quality is degraded in a bright environment due to the extraneous light entering the objective. A room light immediately above the microscope may also enter the objective as extraneous light, especially when using a condenser lens with a longer working distance (i.e. ELWD and LWD). In this case, it is recommended that you turn off the room light above the microscope.

• Install the product at least 10cm away from the surrounding walls. When using TI-DH Dia Pillar Illuminator 100W, install the product at least 15cm away from the wall, so that the caution labels on the dia pillar illuminator and the lamphouse are visible. Note that TI-DH Dia Pillar Illuminator 100W can be inclined backward to secure working space. To utilize this function, install the product so that there is enough space between the product and the wall for the dia pillar illuminator to be inclined.

• Do not install the product in a closed space such as a locker or a cabinet.

• Do not place items on the product.

• Install the product so that the power cord to the power supply can be unplugged immediately in case

of an emergency.

3. Notes on handling optical parts

Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will degrade the microscope image. Handle the optical components with care, so as not to damage them. If they require cleaning, refer to Chapter 6, “Daily Maintenance”.

4. Notes on handling the lamp

Do not touch the lamp glass with your bare hands. Fingerprints and other dirt on the lamp may result in uneven illumination and reduce the service life of the lamp. Wear gloves or use other pieces of clothes when handling the lamp.

Page 10

Safety Precautions

5. Notes on using the focus knobs

• Never rotate the left and right focus knobs in the opposite directions at the same time. Doing so may

damage the product.

• Do not rotate the focus knobs past their limit. Doing so may damage the product.

6. Protecting the ports.

The product has multiple ports. To keep out extraneous light and dust, attach the provided caps to the ports that are not in use.

Page 11

Contents

Introduction...................................................................................................................................................... 1

Safety Precautions .......................................................................................................................................... 2

Warning and Caution Symbols in this Manual............................................................................................................ 2

Symbols on the Product .............................................................................................................................................2

Notes on Handling the Product ..................................................................................................................................7

Contents ........................................................................................................................................................... 9

1. Part Names ..............................................................................................................................................11

1.1 Microscope Configuration .............................................................................................................................12

1.1.1 Ti-U, Ti-U/B.....................................................................................................................................12

1.1.2 Ti-S, Ti-S/L100 ............................................................................................................................... 13

1.2 Microscope Base ..........................................................................................................................................14

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces.................................................................................... 16

1.4 Stage.............................................................................................................................................................18

1.5 Dia Pillar Illuminator ...................................................................................................................................... 19

1.5.1 TI-DH Dia Pillar Illuminator 100W................................................................................................... 19

1.5.2 TI-DS Dia Pillar Illuminator 30W..................................................................................................... 19

1.6 Condenser ....................................................................................................................................................20

1.6.1 TI-C Condenser Turret (System Condenser).................................................................................. 20

1.6.2 ELWD-S Condenser....................................................................................................................... 21

1.7 Power Supply................................................................................................................................................ 22

1.7.1 TI-PS100W Power Supply.............................................................................................................. 22

1.7.2 TE-PS30W Power Supply A (for 100-120V), TE-PSE30 Power Supply A (for 220-240V) .............. 23

2. Microscopy............................................................................................................................................. 24

2.1 Introduction to Microscopy ............................................................................................................................ 25

2.2

Sample Configuration 1:

Ti-U, TI-DH Dia Pillar Illuminator 100W, System Condenser, and TI-TD Eyepiece Tube B

................................. 26

2.2.1 Bright-Field (BF) Microscopy.......................................................................................................... 27

2.2.2 Phase Contrast (Ph) Microscopy.................................................................................................... 34

2.2.3 External Phase Contrast Microscopy .............................................................................................40

2.3 Sample Configuration2:

Ti-S, TI-DH Dia Pillar Illuminator 30W, ELWD-S Condenser, and TI-TS Eyepiece Tube B ...........................45

2.3.1

Bright-Field (BF) Microscopy.......................................................................................................... 46

2.3.2 Phase Contrast (Ph) Microscopy.................................................................................................... 51

3. Operation................................................................................................................................................ 54

3.1 Dia Illumination On/Off.................................................................................................................................. 55

3.2 Brightness Adjustment .................................................................................................................................. 56

3.2.1 Brightness Adjustment with the Brightness Control Knob............................................................... 56

3.2.2 Brightness Adjustment with ND Filters ...........................................................................................57

3.3 Optical Path Selection................................................................................................................................... 58

3.3.1 Ti-U, Ti-U/B Optical Path Selection ................................................................................................ 58

3.3.2 Ti-S, Ti-S/L100 Optical Path Selection ...........................................................................................59

3.3.3 Eyepiece Base Unit Options........................................................................................................... 60

3.4 Filter Operation .............................................................................................................................................61

3.5 Field Diaphragm Operation ........................................................................................................................... 62

3.6 Aperture Diaphragm Operation..................................................................................................................... 63

Page 12

Contents

3.7 Condenser Operation.................................................................................................................................... 65

3.7.1 TI-C Condenser Turret (System Condenser).................................................................................. 65

3.7.2 ELWD-S Condenser....................................................................................................................... 66

3.8 Eyepiece Tube Operation .............................................................................................................................67

3.8.1 Diopter Adjustment ......................................................................................................................... 67

3.8.2 Interpupillary Distance Adjustment .................................................................................................67

3.8.3 Eyepiece Tube Shutter Operation .................................................................................................. 68

3.8.4 Bertrand Lens Operation ................................................................................................................ 68

3.9 Focusing Mechanism Operation ...................................................................................................................69

3.9.1 Coarse Focus Knob and Fine Focus Knob..................................................................................... 69

3.9.2 Torque Adjustment for Coarse Focus Knob .................................................................................... 70

3.9.3 Coarse Focus Stopper Ring (Ti-U, Ti-U/B Only) ............................................................................. 71

3.10 Objective Operation ...................................................................................................................................... 72

3.10.1 Phase Contrast Objectives............................................................................................................. 72

3.10.2 Cover Glass Thickness .................................................................................................................. 72

3.10.3 Objectives with Correction Ring ..................................................................................................... 73

3.10.4 Oil Immersion Objectives ............................................................................................................... 74

3.10.5 Water Immersion Objectives ..........................................................................................................76

3.11 TI-DH Dia Pillar Illuminator 100W Operation ................................................................................................ 77

3.11.1 Condenser Refocusing Clamp ....................................................................................................... 77

3.11.2 Condenser Mount Rotation ............................................................................................................ 77

3.11.3 Pillar Inclination ..............................................................................................................................78

3.11.4 Device Attachment Screw Holes ....................................................................................................78

3.12 TI-SR Rectangular Mechanical Stage........................................................................................................... 79

4. Assembly................................................................................................................................................ 80

5. Troubleshooting .................................................................................................................................. 103

5.1 Image Viewing ............................................................................................................................................103

5.2 Operation .................................................................................................................................................... 105

5.3 Electrical System ........................................................................................................................................105

6. Daily Maintenance ............................................................................................................................... 106

6.1 Cleaning Optical Components ....................................................................................................................106

6.2 Cleaning the Microscope Body ...................................................................................................................106

6.3 Disinfecting the Microscope ........................................................................................................................106

6.4 Storage ....................................................................................................................................................... 106

6.5 Periodic Inspections (Paid Service) ............................................................................................................ 106

7. Specifications ...................................................................................................................................... 107

7.1 Microscope (Ti-U, Ti-U/B, Ti-S, or Ti-S/L100) with TI-DH Dia Pillar Illuminator 100W................................. 107

7.2 Microscope (Ti-U, Ti-U/B, Ti-S, or Ti-S/L100) with TI-DS Dia Pillar Illuminator 30W ................................... 108

7.3 Power Cord (for Power Supply Device) ......................................................................................................109

7.4 Safety Standards Compliance..................................................................................................................... 109

System Diagram............................................................................................................................................110

Ti-U, Ti-U/B System Diagram ................................................................................................................................. 110

Ti-S, Ti-S/L100 System Diagram............................................................................................................................ 112

Page 13

Part Names

This chapter describes the name of each part of the product.

When using the product for the first time, refer to this chapter and check the name and the position of each part. Also refer to this chapter for names and positions of the controls whenever necessary.

• The components of the microscope can be selected to suit the application. However, the lamp, the dia pillar illuminator, and the power supply device must be used in specific combinations. Do not use these devices in an inappropriate combination. For combinations of the lamp, the dia pillar illuminator, and the power supply device, refer to page 55.

• For details on microscopy procedures, see Chapter 2, “Microscopy”. For details on the operation of each part, see Chapter 3, “Operation”.

• If the microscope has not been assembled yet, first refer to Chapter 4, “Assembly”.

Page 14

Chapter 1 Part Names

1.1 Microscope Configuration

1.1.1 Ti-U, Ti-U/B

The illustrations below show the Ti-U microscope body with the following accessories:

TI-DH Dia Pillar Illuminator 100W, TI-C Condenser Turret, D-LH/LC Precentered Lamphouse LC, 12V 100W halogen lamp, TI-PS100W Power Supply, TI-SR Rectangular Mechanical Stage, TI-T-B Eyepiece Base Unit, TI-TD Eyepiece Tube B, CFI 10x eyepieces, TI-ND6 Sextuple DIC Nosepiece, objectives, etc.

PORT

Figure 1-1 Ti-U

OFF

6V30W

MAX.

12V100W

Figure 1-2 Ti-U (Left View)

* Attach a protective cap on the side port when the side port is not in use.

Dia illumination brightness control knob

Dia illumination ON/OFF switch

Left side port*

Focus knob

(Coarse/Fine)

Torque adjustment ring for coarse focus knob

Lamphouse for dia illumination (Fig.: D-LH/LC)

Filter sliders (both sides, 4 total)

Dia pillar illuminator

(Fig

.: TI-DH)

Condenser mount

Stage (Fig.: TI-SR)

Nosepiece (Fig.: TI-ND6)

Stage knob

Right side port

Focus knob (Coarse/Fine)

Coarse focus stopper ring

Intermediate magnification

selector knob

(1.5x <

---

> 1x)

Microscope base

(Fig.: Ti-U)

Power supply device

(Fig.: TI-PS100W)

Eyepiece base unit (Fig.: TI-T-B)

Eyepiece tube (Fig.: TI-TD)

Eyepieces (both sides)

Field diaphragm knob

Condenser lens

Condenser turret (Fig.: TI-C)

Condenser centering knob (both sides)

Aperture diaphragm open/close leve

Condenser focus knobs (both sides)

Objectives Stage ring (concentric ring)

Optical path

selector knob

Page 15

Chapter 1 Part Names

1.1 Microscope Configuration

1.1.2 Ti-S, Ti-S/L100

The illustrations below show the Ti-S microscope body with the following accessories:

TI-DS Dia Pillar Illuminator 30W, ELWD-S Condenser, TE-PS30 30W Power Supply, TI-SR Rectangular Mechanical Stage, TI-T-B Eyepiece Base Unit, TI-TS Eyepiece Tube B, CFI 10x eyepieces, TI-N6 Sextuple Nosepiece, objectives, etc.

PORT

SIDE

EYE

DS 30W

6V 30W HALOGEN

Figure 1-3 Ti-S

OFF

6V30W

MAX.

12V100W

Figure 1-4 Ti-S (Left View)

* Attach a protective cap on the side port when the side port is not in use.

Lamphouse

Dia pillar illuminator (Fig.: TI-DS)

Filter sliders

Stage (Fig.: TI-SR)

Nosepiece (Fig.: TI-N6)

Stage knob

Optical path selector knob

Main body (Fig.: Ti-S)

Power supply device

(Fig.: TI-PS30W)

Eyepiece base unit

(Fig.: TI-T-B)

Eyepiece tube (Fig.: TI-TS)

Eyepieces (both sides)

Condenser lens

Condenser turret (Fig.: ELWD-S)

Condenser holde

Objectives Stage ring (concentric ring)

Field diaphragm slider

Focus knob (Coarse/Fine)

Dia illumination brightness control knob

Dia illumination ON/OFF switch

Left side port*

Torque adjustment ring for coarse focus knob

Focus knob

(Coarse/Fine)

Dustproof slider

Page 16

Chapter 1 Part Names

1.2 Microscope Base

To keep out extraneous light and dust, be sure to attach the provided caps to all ports not in use.

PORT

Figure 1-5 Microscope Base

Optical Path Selector Knob Operation

Ti series microscopes have multiple output ports. Use the optical path selector knob to select the output port for the microscope image.

Ti-U Ti-U/B Ti-S Ti-S/L100

EYE Visual observation

100%

R Right side port

100%

AUX Prism attachment

position

L Left side port 100%

EYE Visual observation

100%

R Right side 100%

B Bottom port 100%

L Left side 100%

EYE Visual 100%

SIDE Visual observation

20%, Left side port 80%

EYE Visual observation

100%

SIDE Left side port 100%

Dia pillar illuminator mount (A carrying handle is attached for transportation)

Connector panel (rear)

Right side port (Ti-U and Ti-U/B only)

Fine focus knob

Bottom port (on the bottom, Ti-U/B only)

Intermediate magnification

selector knob

(1.5x <

---

> 1x)

(Ti-U and Ti-U/B only)

Ti-U/B has a front optical

path selector knob fo

the bottom port

Optical path

selector knob

Eyepiece base unit

mount

Nosepiece mount

Cove

nalyzer slot

dummy slider is attached

for trans

ortation.

Coarse focus knob

Coarse focus stopper ring (Ti-U and Ti-U/B only)

Filter turret mount

Page 17

Chapter 1 Part Names

1.2 Microscope Base

Left View

6V30W

MAX.MIN.

12V100W

OFF

Figure 1-6 Microscope Base (Left View)

Rear View (Connector Panel)

INTERLOCK

LAMP CTRL

Gepruftes Medizinprodukt Approved medical device

Freiwillige Produktprufung

5 3 2 0 0 1

This device complies with Part 15 of the FCC Rules.Operation is subject to the following two conditions: (1) this device may not cause harmful interference,and (2)

EQUIPMENT 4N75 MODEL : Ti-U

INSPECTION

TUV Rheinland

Product Safety

JAPAN

this device must accept any interference received, including interference that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numØrique de la classe A est conforme  la norme NMB-003 du Canada.

Figure 1-7 Microscope Base (Rear View)

Epi-fl illumination field

diaphragm slide

mount

Fine focus knob, Coarse focus knob, Torque adjustment ring for coarse focus knob

Left side port

Dia illumination lamp ON/OFF switch

Dia illumination brightness control knob

Epi-fl illumination mount

LAMP CTRL connector (for dia illumination power supply)

INTERLOCK connecto

(for external laser controller)

(Ti-U and Ti-U/B only)

Hub Controller B attachment

screw holes (x4)

Power cable groove

(both sides)

Epi-fl illumination attachment screw holes (x4)

Page 18

Chapter 1 Part Names

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces

The following eyepiece base units, eyepiece tubes, and eyepieces can be mounted on the observation port of the microscope.

TI-T-B Eyepiece Base Unit

Figure 1-8 TI-T-B Eyepiece Base Unit

Basic eyepiece base unit.

circular dovetail joint is provided on the top surface for eyepiece tube attachment. Attach an eyepiece tube for visual observation.

If you will not be performing visual observation, you do not need to attach an eyepiece tube. In this case, be sure to attach a cover onto the circular dovetail joint.

TI-T-BS Eyepiece Base Unit (with Side Port)

Figure 1-9 TI-T-BS Eyepiece Base Unit

Eyepiece base unit with a camera port.

Rotate the optical path selector knob on the right to distribute the light between the eyepiece tube and the side port.

The side port is equipped with a C-mount adapter for attaching microscopy cameras.

TI-T-BPH External Phase Contrast Eyepiece Base Unit

Figure 1-10 TI-T-BPH Eyepiece Base Unit

Eyepiece base unit for external phase contrast microscopy.

A turret for phase contrast rings is provided on the front of the eyepiece base unit. The turret has three positions (A, B, and C) that can each hold an external phase contrast phase plate. The focus can be adjusted independently for each position. The turret has screws on its sides for centering the phase contrast rings, and clamp screw on its back for fixing the adjusted position.

On the left side, a side port with a C-mount adapter is provided. On the right side, an optical path selector knob is provided for light distribution between the eyepiece tube and the side port.

When performing photomicroscopy during external phase contrast microscopy, use this side port.

Circular dovetail

oint

Eyepiece tube locking screw

Centering screws

(2 positions)

Optical path

selector knob

Focusing screws

Circular dovetail

oint Eyepiece tube locking screw

Circular dovetail joint

Eyepiece tube locking screw

Side port

(with C-mount adapter)

Optical path selector knob

Stage mount

Eyepiece tube

mount

Microscope moun

(bottom)

Stage mount

Eyepiece tube

mount

Microscope mount

(bottom)

Stage mount

Eyepiece tube mount

Side port

(with C-mount adapter)

Centering clamp screw (back, 2 positions)

Microscope mount (bottom)

Turret

Page 19

Chapter 1 Part Names

1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces

TI-TS Eyepiece Tube B

Figure 1-11 TI-TS Eyepiece Tube B

Simple and basic eyepiece tube.

TI-TD Eyepiece Tube B

Figure 1-12 TI-TD Eyepiece Tube B

Eyepiece tube with a manual shutter and a Bertrand lens.

The shutter can be closed to cut off the optical path to the eyepieces, for example when observing with a camera.

The Bertrand lens can be moved into the optical path for observation of the objective pupil plane.

TI-TERG Ergonomic Eyepiece Tube

Figure 1-13 TI-TERG Ergonomic Eyepiece Tube

Eyepiece tube with an adjustable binocular that can be inclined to fit the user.

Like TI-TD Eyepiece Tube B, this eyepiece tube is equipped with a Bertrand lens. The optical path to the eyepieces can be cut off, for example when observing with a camera. The Bertrand lens can be moved into the optical path for observation of the objective pupil plane.

Eyepieces

(both sides,

sold separately)

Binocular part

Eyepieces

(both sides,

sold separately)

Binocular part

Shutter operation lever

Bertrand lens focusing knob

Bertrand lens

in/out leve

Eyepieces

(both sides, sold

separately)

Binocular part

Shutter operation lever

Bertrand lens focusing knob

Bertrand lens

in/out leve

Page 20

Chapter 1 Part Names

1.4 Stage

The following stages can be attached to the product.

TI-SR Rectangular Mechanical Stage

Figure 1-14 Rectangular Mechanical Stage

The specimen can be moved in the X and Y directions by operating the stage knob.

The rectangular mechanical stage comes with two stage clips for culture vessels, and the following two concentric rings.

25 mm bore

(with oiling notch)

40 mm bore

TI-SP Plain Stage

Figure 1-15 Plain Stage

Simple, fixed stage for easy operation of specimens.

The plain stage comes with the following two concentric rings.

25 mm bore

(with oiling notch)

40 mm bore

TI-SAM Attachable Mechanical Stage

Figure 1-16 Attachable Mechanical Stage

(on Plain Stage)

This stage is attached to the plain stage when using specimen holders. The specimen can be moved in the X and Y directions by operating the stage knob.

Various specimen holders can be used by attaching the following adapters to the provided microplate.

• MA60 microplate holder

• MA60 Petri dish holder

• MA glass slide holder

• 35 Petri dish holder

Stage knob

・Y-axis direction

・X-axis direction

Plain stage

ttachable mechanical stage

Stage ring (concentric ring)

Rack

Stage knob

・Y-axis direction ・X-axis direction

Stage ring (concentric ring)

Page 21

Chapter 1 Part Names

1.5 Dia Pillar Illuminator

Ti series microscopes can be used with the following two types of dia pillar illuminators. The two dia pillar illuminators differ in lamp rating (12V 100W or 6V 30W) and support different microscopy methods. Select the dia pillar illuminator to suit your application.

1.5.1 TI-DH Dia Pillar Illuminator 100W

The TI-DH Dia Pillar Illuminator 100W is used with a separate lamphouse and condenser. The following illustration shows the TI-DH Dia Pillar Illuminator 100W with the D-LH/LC Precentered Lamphouse and the TI-C Condenser Turret (System Condenser) attached.

Figure 1-17 TI-DH Dia Pillar Illuminator 100W

(with Lamphouse and System Condenser)

1.5.2 TI-DS Dia Pillar Illuminator 30W

The TI-DS Dia Pillar Illuminator 30W has a built-in lamphouse, but requires a separate condenser. The following illustration shows the TI-DS Dia Pillar Illuminator 30W with the ELWD-S Condenser.

DS 30W

30W

HALOGEN

Figure 1-18 TI-DS Dia Pillar Illuminator 30W

(with ELWD-S Condenser)

D-LH/LC Precentered Lamphouse LC Requires TI-PS100W power supply

Lamp cable

Condenser mount

(rotatable)

Condenser mount

rotation clamp scre

Condenser centering

knob

Condenser lens

TI-C System Condenser Turret

Condenser clamp scre

Field diaphragm knob

Pillar locking clamp knob

Filter sliders (both sides, 4 total)

Condenser refocusing clamp

Condenser focus knobs (both sides)

Condenser holder (detachable)

Condenser holder clamp screw

Field diaphragm slider

Lamphouse (6V 30W lamp, sold separately) (Requires TE-PS30W or TE-PSE30 power supply.)

Filter sliders (max. 3)

Dustproof slide

Device attachment screw holes (M4, x8)

Condenser mount

Condenser holde

ELWD-S Condense

Page 22

Chapter 1 Part Names

1.6 Condenser

1.6.1 TI-C Condenser Turret (System Condenser)

The TI-C Condenser Turret allows you to attach various optical elements to a turret and select them as necessary for different microscopy methods. This type of condenser is referred to as a “system condenser”.

System Condenser (Mounted on TI-DH Dia Pillar Illuminator 100W)

Figure 1-19 TI-C Condenser Turret

(mounted on TI-DH Dia Pillar Illuminator 100W)

System Condenser (Mounted on TI-DS Dia Pillar Illuminator 30W)

Figure 1-20 TI-C Condenser Turret

(mounted on TI-DS Dia Pillar Illuminator 30W)

* The system condenser can be mounted on the TI-DS Dia Pillar Illuminator 30W for HMC observation only.

Condenser focus knob

Condenser centering knob

Condenser lens

(4 types: ELWD, LWD,

HMC, and CLWD)

Condenser cassette clamp screws

(

both sides of cassette)

Condenser turret

Condenser cassettes

(Ph condenser cassettes have phase contrast ring centering screws on the sides.)

Aperture diaphragm

open/close leve

Condenser holde

Condenser lens

(for HMC only)

Aperture diaphragm

en/close leve

Condenser cassette clamp screws (both sides of cassette)

Condenser turret

Condenser cassettes

Page 23

Chapter 1 Part Names

1.6 Condenser

1.6.2 ELWD-S Condenser

Figure 1-21 ELWD-S Condenser

The ELWD-S Condenser supports bright-field microscopy and phase contrast microscopy. It can be used with both 100W and 30W dia pillar illuminators.

Condenser lens

Turret

Centering knob

Clamp screws

(both sides)

Condense

Centering knobs

(

both sides

)

Page 24

Chapter 1 Part Names

1.7 Power Supply

1.7.1 TI-PS100W Power Supply

Warning

The bottom of the power supply device becomes hot during use. Do not block the air vents on the side of the product.

Figure 1-22 TI-PS100W Power Supply

POWER switch

This is the power switch for the power supply. Press the “I” side of the switch to turn on the power supply and output DC power from the 12VDC output on the rear. Press the “O” side of the switch to turn off the power supply.

POWER indicator

Lit when the power supply is on.

Brightness control knob

When the EXTERNAL switch is turned off, the knob controls the brightness of the lamp by adjusting the voltage supplied from the 12VDC output connector.

AC inlet

This is the connector for connecting the power supply device to a wall outlet. Be sure to use the specified power cord for the connection.

EXTERNAL (external control) ON/OFF switch

Turn this switch on to use the brightness control knob on the microscope for output voltage control. When this switch is turned off, the brightness control knob on the front of the power supply becomes enabled, and the brightness control knob on the microscope becomes disabled.

EXTERNAL (external control signal input) connector

Connect the control cable to this connector and the LAMP CTRL connector on the rear of the microscope.

Pin Signal

External resistor terminal for output voltage adjustment

Output voltage ON/OFF switch (input)

GND (0V)

External voltage input for output voltage adjustment

EXTERNAL switch on/off detect signal (output)

GND (0V)

Output voltage monitor terminal (output)

Connector: HR12-10R-8SC by Hirose Electric Co., Ltd.

12VDC output connector

This connector supplies power to the 12V 100W halogen lamp. Connect the lamp cable for the pillar illuminator.

Pin Signal

Output +

Output -

Not used

Connector: SRCN2A13-3S by Japan Aviation Electronics Industry, Ltd.

POWER switch

POWER indicato

Brightness

control knob

AC inlet

12VDC output connecto

EXTERNAL connector

EXTERNAL ON/OFF switch

Page 25

Chapter 1 Part Names

1.7 Power Supply

1.7.2 TE-PS30W Power Supply A (for 100-120V)

TE-PSE30 Power Supply A (for 220-240V)

Warning

• Before turning on the power supply, check that the input voltage indicator matches the power voltage in your area. If the voltages do not match, do not turn on the product, and contact Nikon. Use of the product under a wrong voltage may result in malfunction or fire.

• The bottom of the power supply device becomes hot during use. Do not block the air vents on the side of the product.

Figure 1-23 TE-PS30W, TE-PSE30 Power Supply A

POWER switch/indicator

This is the power switch for the power supply. Press the “I” side of the switch to turn on the power supply and output DC power from the 6VDC output on the rear. The switch is lit when the power supply is on. Press the “O” side of the switch to turn off the power supply.

Brightness control knob

When the CTRL switch is turned off, this knob controls the brightness of the lamp by adjusting the voltage output from the 6VDC output connector.

AC inlet

This is the connector for connecting the power supply device to a wall outlet. Be sure to use the specified power cord for the connection.

CTRL (external control) ON/OFF switch

CTRL (external control signal input) connector

Connect the control cable to this connector and the LAMP CTRL connector on the rear of the microscope.

Pin Signal

External resistor terminal for output voltage adjustment

Output voltage ON/OFF switch (input)

GND (0V)

Not used

Connector: HR12-10R-8SC by Hirose Electric Co., Ltd.

6VDC output connector

This connector supplies power to the 6V 30W halogen lamp. Connect the lamp cable for the pillar illuminator.

Pin Signal

Output -

Not used

Output +

Connector: SRCN2A13-3S by Japan Aviation Electronics Industry, Ltd.

Brightness control

knob

AC inlet

6VDC output connector CTRL connector

CTRL ON/OFF switch

POWER

switch/indicato