Thank you for purchasing a Nikon product.
This instruction manual is written for users of Nikon Stereoscopic Zoom
Microscope SMZ745T.
To ensure correct usage, read this manual carefully before operating the
product.
• No part of this manual may be reproduced or transmitted in any form
without prior written permission from Nikon.
• The contents of this manual are subject to change without notice.
• Although every effort has been made to ensure the accuracy of this
manual, errors or inconsistencies may remain. If you note any points
that are unclear or incorrect, please contact your nearest Nikon
representative.
• Some of the equipment described in this manual may not be included in
the set you have purchased.
• If you intend to use any other equipment with this product, read the
manual for that equipment too.
• If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
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Safety Precautions
To ensure correct and safe operation, read this manual before using the product.
Warning/Caution Symbols Used in this Manual
Although this product is designed and manufactured to be
completely safe during use, incorrect usage or failure to follow the
safety instructions provided may cause personal injury or property
damage. To ensure correct usage, read this manual carefully before
using the product. Do not discard this manual and keep it handy for
easy reference.
Safety instructions in this manual are marked with the following
symbols to highlight their importance. For your safety, always
follow the instructions marked with these symbols.
SymbolDescription
WARNING
CAUTION
Disregarding instructions marked with this
symbol may lead to serious injury or death.
Disregarding instructions marked with this
symbol may lead to injury or property
damage.
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WARNING
1. Intended use of this product
This product is intended only for microscopy. Do not use it for any
other purposes.
2. Do not disassemble.
Disassembling this product may result in electric shock or
malfunction. Do not disassemble any part that is not indicated in
this manual. If you experience problems with the product, please
contact your nearest Nikon representative.
3. Read the instructions thoroughly.
To ensure safety, thoroughly read this manual and the manuals for
other equipment to be used with this product. In particular, be sure
to follow the warnings and cautions at the beginning of the manuals.
4. Check the input voltage
When using an illuminator, check that the input voltage displayed
on the power supply of the illuminator matches the operating
voltage. Please contact your nearest Nikon representative if the
displayed voltage does not match the operating voltage. Use of an
improperly matched illuminator may result in damage to the
equipment.
5. Cautions on the power cord
The power supply is necessary for using the halogen illuminators,
which are accessories. Follow the cautions below for the power
cord.
• To prevent electric shock, turn off the power switch on the power
supply before connecting or disconnecting the power cord.
• For the power supply TN-PSE30W A (for regions with 230 V
power only), the power code is specified. Be sure to use the
specified power cord for the power supply. Use of other power
cords may result in malfunction, overheat, or fire.
(Refer to Power Supply TN-PSE30W A (for regions with 230
V power only) on p.22.)
3
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WARNING
6. Cautions on heat from the light source
The lamp and the lamphouse become extremely hot when the lamp
is turned on. Follow the cautions below to prevent burns and fire.
• To avoid burns, do not touch the lamp and the lamphouse while
the lamp is on or for approximately thirty minutes after it has
been turned off.
• To avoid the risk of fire, do not place fabric, paper, or highly
flammable volatile materials (i.e. gasoline, petroleum benzine,
paint thinner, and alcohol) near the lamphouse while the lamp is
on or for about thirty minutes after it has been turned off.
7. Cautions on lamp replacement
• When replacing the lamp, wait approximately thirty minutes
after turning off the lamp, and make sure that the lamp and the
lamphouse have cooled sufficiently.
• To prevent electric shock and product damage, turn off the power
switch on the power supply before replacing the lamp.
CAUTION
1. Check the light source
When using an illuminator, be sure to use the specified rating only.
Use of other rating may result in damage to the equipment. Refer to
the instructions for the lamp rating.
2. Cautions on assembling the product
• Take care to avoid pinching your fingers and hands.
• Scratches and dirt (i.e. fingerprints) on the lenses will degrade
the image quality. When assembling the product, be careful not
to scratch or directly touch the lenses.
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Notes on Handling the Product
1. Handle with care.
This stereoscopic microscope is a precision optical instrument.
Handle the product with care and avoid physical shocks. Physical
shocks during transportation and operation as well as forcible
operations may result in damage to the product.
2. Installation location
Note the following conditions when installing the product.
• Install the product in a location with a temperature of 0 to +40ºC
and a relative humidity of 85% or less (no condensation).
If installed in a hot or humid location, mold may form on the
lenses or condensation may occur inside, resulting in poor
performance or damage to the product.
• Do not use the product in a location subject to direct sunlight.
• Install the product in a location with little vibration.
• Install the product in a location with little dust and dirt.
Safety Precautions
3. Cleaning the lenses
Do not allow dust, fingerprints, or any other dirt to get on the lenses.
Dirt on the lenses will degrade the image quality. If a lense becomes
dirty, clean them as described below.
• Use an air blower to blow dust away. Remove any dust by brushing
off with a soft brush or by wiping gently with a gauze, if necessary.
• Only when there are fingerprints or grease on the lense, wipe
gently with a soft, clean cotton cloth, lens tissue, or gauze
dampened with a small amount of absolute alcohol (ethyl or
methyl). Do not use the same part of the cloth more than once.
• Absolute alcohol is highly flammable. Handle with care. Do not
use near an open flame.
• When using absolute alcohol, follow the instructions provided by
the manufacturer.
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Safety Precautions
4. Cleaning painted or plastic parts
Use of silicon cloth is recommended for cleaning painted parts,
plastic parts and printed parts. If such a part becomes excessively
dirty, wipe it gently with a gauze dampened in a mild detergent
solution. Do not use organic solvents (such as alcohol, ether, or paint
thinner). As this may result in deformation of the part or removal of
lettering.
5. Storage
• Store the product in a dry location where mold is unlikely to grow.
• Do not store the product in a location subject to direct sunlight or
high temperature and humidity.
• Put a dust-proof cover over the product to protect it from dust.
6. Regular inspections (Paid service)
Regular inspections are recommended in order to maintain the peak
performance. Please consult your nearest Nikon representative for
details about regular inspections.
The illustration shows the combination of SMZ745T zooming body, C-W10X
eyepieces and C-PS plain focusing stand.
C mount fixing screw
The direction of DS
camera head can be
adjusted by
loosening the fixing
screw and rotating
the C mount.
Vertical tube
Eyepiece
10X, 15X, 20X and 30X
eyepieces are available.
Diopter ring
Refer to “ 4. Adjust the
diopter.” on p.11.
Zooming body
Outside diameter of the part
to be mounted on the arm:
ø76
Zooming body clamp screw
Fixes the zooming body to
the arm.
Arm
Groove for mounting
accessories
Holds the fluorescent
ring illuminator and the
fiber optic ring
illuminator etc.
Outside diameter: ø60
Screw for mounting
accessories
Holds auxiliary
objectives etc.
Inside diameter screw:
M55 × 0.75
Stage plate
The black or white side can be chosen
depending on the sample. The antistatic
ESD stage plate is also available.
C mount cap
C mount
Nikon DS camera
head can be
attached.
Optical path
switching lever
Changes the light
distribution between
the vertical tube and
the right eyepiece
tube. Refer to “6.
Change the optical
path.” on p.12.
Sleeve
Zooming knob
Changes the
magnification of the
sample image.
Refer to “5. Change
the zooming
magnification.” on
p.11.
Focus knob
Refer to “2. Focus on
the sample.” on p.10.
Arm fixing screw
Holds the arm. The
height of the arm can
be adjusted high or
low.
Vertical slider
Hole for clip
Holds the clip for
retaining the sample.
C-PS Focusing stand
Various stands available.
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Page 11
Eyepiece inclination
45° from the horizontal
Screw hole for mounting an
arm for halogen illuminator
Screw holes are located on
both sides of the arm. Holds
the G-EIA flexible arm or
Epi-arm.
Screw hole for mounting
6V10W halogen illuminator
Holds the G-LS 6V10W
halogen illuminator.
I
Zooming knob clicks ON/OFF screw
(inside)
Refer to “ON/OFF of the
zooming knob clicks.” on p.11
Hexagonal wrench
hanger
Stores the supplied
hexagonal
wrenches.
Hexagonal wrench
(Large)
Supplied with the
focusing stand for
changing the
height of the arm.
Hexagonal wrench
(Small)
Supplied with the
zooming body for
switching ON/OFF
the zooming knob
clicks.
Pillar
Ground jack
Refer to “Antistatic
Feature” on p.15.
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Page 12
Microscopy Procedures
II
• If the microscope has not been assembled, first refer to Chapter III, “Assembly.”
Place the sample.
1
Place the sample in the optical path on the stage plate of the stand.
The black or white side can be chosen depending on the sample.
Use the stage clips to hold the sample, if necessary.
Focus on the sample.
2
Turning the left and right focus knobs to the same direction will raise or
lower the arm (on which the zooming body is mounted) and focus on the
sample.
■ Working distance
The distance between the focus plane and the bottom surface of the
zooming body is called “the working distance”. Since the working
distance of the SMZ745T is 115 mm, the focusing will become easier if
you set the zooming body at the position where its bottom surface is 115
mm apart from the sample surface. Refer to Table 1 (p.23) for changing
the working distance when the auxiliary objectives are attached.
■ The torque of the focus knob
If the torque of the focus knob is too light, the
zooming body falls down by its own weight.
Adjust the torque to an appropriate weight.
(Refer to “7. Adjust the torque of the focus
knob.” on p.14.)
10
Adjust the interpupillary distance.
3
This adjustment is for adjusting the distance
between both eyes of the observer.
Adjust the interpupillary distance so that the
fields of view for each eye are merged into
one. Move while holding each sleeve with
both hands.
This adjustment is required for each observer
since individual interpupillary distance vary.
The arm moves down by turning
the focus knob towards the
observer.
Page 13
Adjust the diopter.
4
This adjustment is for adjusting the eyesight of the observer.
1 Turn the diopter rings on both eyepieces to set them at the 0 position
(match the 0 line with the index line).
2 Turn the zooming knob to 5X. Focus on the sample using the focus
knob. (Refer to “2. Focus on the sample.” on p.10.)
3 Turn the zooming knob to 0.67X. Look into the left eyepiece with
the left eye, and focus on the sample using the diopter ring on the
left eyepiece. Then, look into the right eyepiece with your right eye
and focus on the sample using the diopter ring on the right eyepiece.
4 Repeat steps 2 and 3 until the image is kept focused even though the
zooming magnification is changed. This adjustment ensures the
sharp image throughout the zooming range.
This adjustment should be performed each time the observer is changed
since individual eyesights vary.
Change the zooming magnification.
5
The magnification of the sample image will change by turning the left and
right zooming knobs of the zooming body. Choose the desired
magnification.
II
■ Total magnification
The right zooming knob from the viewing side has the indication of the
zooming magnification. Total magnification can be calculated by
multiplying the eyepiece magnification by the zooming magnification.
Note) When the auxiliary objective is attached,
multiply its magnification as well.
■ ON/OFF of the zooming knob clicks
The clicks on the ON/OFF of the zooming
knob are available.
1 Remove the small rubber cap from the
rear of the zooming body.
2 Insert the supplied hexagonal wrench
(small) into the screw hole on the rear of
the zooming body.
Zooming
knob
ON
OFF
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Page 14
Microscopy Procedures
Auxiliary
adapter
0.5X Auxiliary
objective
3 ON/OFF of the zooming knob clicks are adjustable by turning the
inside screw.
4 Return the small rubber cap to its original position.
II
Change the optical path.
6
The light distribution between the vertical
tube and the right eyepiece tube can be
switched with the optical path switching lever.
For binocular observation, move the lever
foward. When Nikon DS camera head is
mounted to the C mount of the zooming body
for taking microphotographs, move the lever
backward.
Please refer to the instruction of the camera
head for the details of the Nikon DS Camera
Head operation.
• Refer to Table 2 (p.24) for Total Magnification and Real Field of
☞
Vertical Tube.
Image cannot be focused even with the
7
zooming body at the highest position.
For using the 0.5X auxiliary objective or for
observing a tall sample, you may not be able
to focus on the sample even if turning the
focus knob to raise the zooming body to its
highest position.
In this case, use an optional C-ER auxiliary
adapter.
Optical path
switching lever
Right eyepiece : Vertical tube
=100 : 0
Right eyepiece : Vertical tube
=100 : 0
12
Note) Attach the auxiliary objective first to
the zooming body, then mount the
zooming body on the auxiliary adapter.
• Refer to Table 3 (p.24) for Observable Sample Heights.
☞
Page 15
III
Assembly
Place the stand on the level surface.
1
Mount the stage plate.
2
Press the stage plate into the stand base while
pushing it against the rim in the direction
shown by the arrow in the illustration.
The stand arm can be lowered.
3
(For not lowering the arm, jump to
step 4.)
Use the hexagonal wrench (large, supplied
with the focusing stand) to loosen the arm
fixing screw. Reattach the arm using the
screw hole at the lower side of the vertical
slider. The arm will become 55 mm lower
than its original position. Before tightening
the screw, make sure that the two pins on the
arm are inserted into the grooves in the
vertical slider.
Groove
Pin
Mount the zooming body.
4
Lightly tighten the zooming body clamp
screw to hold the zooming body on the arm.
Note) Do not overly tighten the zooming body
clamp screw since it may cause
damage to the product.
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Page 16
Insert the eyepieces into the eyepiece sleeves.
5
Be sure that it is inserted all the way to the end of the sleeve.
Note) When inserting the 10X eyepiece, assure that it reaches the end of
the sleeve, because the rubber cover of the 10X eyepiece will
obstruct the view of the sleeve end. When inserting, hold the rubber
cover instead of the diopter ring. If not, it may result in the damage
to the product.
Attach the camera head (optional).
6
Remove the C mount cap from the zooming
body.
Mount the C mount adapter to DS camera
head first. Then, attach the DS camera head to
the C mount of the zooming body.
The direction of DS camera head can be
adjusted by loosening the fixing screw and
rotating the C mount.
14
Adjust the torque of the focus knob.
7
Increase the torque
Adjust the torque of the focus knob so as not
to fall down the zooming body by its own
weight.
(To reduce the torque,
turn the knob in the direction
opposite to the arrow.)
Storing the tools
8
The hexagonal wrenches can be stored at the rear of the focusing stand.
Refer to the figure on p.9.
Page 17
Assembly
Antistatic Feature
The SMZ745T, the C-PS/C-PSC plain focusing stand and the C-W10X
eyepieces are antistatic. You can enjoy this feature when observing the sample
that cannot stand much static. When using the microscope on this purpose,
change the stage plate to antistatic ESD stage plate and ground the microscope
through the ground jack at the rear of the plain focusing stand. The φ 4 mm
grounding terminal can be inserted into the ground jack.
The following accessories are also antistatic.
• C-FPS Fluorescence Attachment
• Auxiliary Objectives (all types)
• C-FMB Focusing Mount B
• G-US1 and G-US2 Universal Table Stands
III
15
Page 18
IV
Using Accessories
Reticles
1
Your reticles can be attached to the eyepiece.
Remove the field ring (or lens room of the
20X and 30X) from the eyepiece, then attach
the reticle with its pattern surface facing down
to the eyepiece, and reattach the field ring (or
lens room).
• Refer to Table 1 (p.23) for the size of
☞
the reticle which can be attached.
Note) The 20X and 30X eyepieces adopt the
internal focusing system, and the
primary image has the magnification.
Use the reticle with 1.3X pattern for
20X eyepiece, and with 1.4X pattern
for 30X eyepiece.
An optional reticle with cross hairs or
scaled cross hairs is available for 10X
eyepiece.
Reticle
Pattern
surface
Optional
reticle
Field ring
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Page 19
Halogen Illuminators
2
There are two illuminators available: the G-LS of 6V10W and the
C-DSLS of 6V20W.
G-LS
Lamp Socket A (6V10W)
Lamphouse
Lamp socket
(Removable
back and forth)
Mounting screw
C-DSLS
Illuminator A (6V20W)
IV
Plug
PlugLamphouseLamp socket
1
Filter cap
ø33 mm filter
attachable
Holder and Epi arm
mounting screw hole
Lamp
WARNING
• When replacing the lamp, wait approximately thirty minutes
after turning off the lamp, and make sure that the lamp and the
lamphouse have cooled sufficiently.
• To prevent electric shock and product damage, turn off the
power switch on the power supply before replacing the lamp.
17
Page 20
Pull the lamp socket from the lamphouse and insert the lamp straight into
the socket to the end. Use halogen lamps of the specified rating only.
Then, attach the lamp socket to the lamphouse.
G-LS
Socket
Lamp
(GE 787 6V-10W)
C-DSLS
Confirmation
window
Lamp
(KLS JCR/M
6V20W-2)
18
Light
shielding tube
SocketSmall protrusion
Holder mounting
screw hole
Note) Do not touch the glass part of the lamp with bare hands as it may
burn and stick to your skin, possibly reducing its level of light
output or causing damage to the lamp. When replacing a lamp,
handle it with a cloth or through its packaging until the
replacement is completed. If you get fingerprints on the lamp, wipe
gently with a clean cloth dampened with alcohol.
Page 21
Mounting screw
Flexible
arm
Holder
Protrusion
G-LS
Using Accessories
Attaching to the stand (for G-LS only)
2
Align the protrusion on the lamphouse with
the inside of the screw hole on the arm of the
G-LS
stand (screw hole for mounting 6V10W
halogen illuminator), then insert the mounting
screw from the outside of the arm and secure
it.
Screw hole for
mounting 6V10W
halogen illuminator
Attaching to the stand with arm for halogen illuminator
3
Special arms are provided to be attached to the stand. By using these arms,
the direction of illumination can be controlled easily.
1 G-EIA flexible arm (for G-LS only)
Align the protrusion on the lamphouse
with the inside of the holder of the
flexible arm, then insert the mounting
screw from the outside of the holder and
secure it.
IV
Mounting
screw
Secure the other end of flexible arm to
the left or right screw hole (for
mounting an arm for halogen
illuminator) on the arm of the stand by
the mounting screw supplied with the
flexible arm.
Flexible arm
Mounting screw
19
Page 22
2 Epi arm
Slightly loosen the clamp knob on the
arm. Screw in the mounting screw at
the end of the Epi arm into the left or
right screw hole (for mounting an arm
Arm
Joint
for halogen illuminator) on the arm of
the stand while turning the joint. Then,
securely tighten the nut using the
supplied wrench.
Mounting
screw
Screw the mounting screw at the other
end of the Epi arm into the screw hole
on the lamphouse as described above.
Set the lamphouse in a position for easy
viewing, and tighten the clamp knob.
Lighting area adjustment (for G-LS only)
4
You can adjust the lighting area by moving the lamp socket back and
forth. Adjust the lighting area to allow easy viewing.
Clamp knob
Simultaneously
clamps two
arms/joints.
Nut
Wrench
20
Attaching filters (for C-DSLS only)
5
Rotate and remove the filter cap at the end of
the lamphouse and insert a filter. Use filters
that have a diameter of 33 mm and a width of
1.5 to 2 mm.
Filter
ø33 mm
Filter cap
Page 23
Using Accessories
Power supplies
6
Use the Power Supply XN A for regions using 100 to 120 V power, and
the Power Supply TN-PSE30W A for regions using 230 V power. Insert
the plug of the halogen illuminator into the output connector located on
the rear of the power supply.
Power Supply XN A (for regions with 100 to 120 V power only)
IV
Input voltage display
Check that the voltage displayed matches the
voltage used in your area. If the voltages do
not match, do not use the power supply and
contact your nearest Nikon representative.
4
5
3
6
V
O
L
T
A
G
E
Fuse
Always use the specified
fuse (250 V, 1 A).
(Rear view)
Power cord
Power switch
Brightness adjuster
Changes the illumination
brightness when rotated.
Output connector
Connect the
lamphouse cable here.
Input ratingPower supply for 100 V regions only:
100 VAC, 50/60 Hz, 30 W
Power supply for 120 V regions only:
120 VAC, 50/60 Hz, 30 W
Output rating3 to 6 VAC, 3.3 A
Fuse rating250 V, 1 A
21
Page 24
Using Accessories
IV
Power Supply TN-PSE30W A (for regions with 230 V power only)
Brightness adjuster
Changes the illumination
brightness when rotated.
Input voltage display
Check that the voltage displayed matches the
voltage used in your area. If the voltages do
not match, do not use the power supply and
contact your nearest Nikon representative.
Output connector
Connect the
(Rear view)
lamphouse cable here.
Power switch
Power cord
AC input connector
Connect the specified power cord here.
Input rating230 VAC, 50/60Hz, 0.3 A
Voltage fluctuation±10%
Output rating6 VAC, 5 A
Internal fuseFast acting type, F1A/250 V, 5.2 × 20 mm
OperatingAltitude:2000 m max.
environmentTemperature:0 to +40°C
(indoor use only)
Relative humidity: 85% RH max.
(no condensation)
Pollution level:Degree 2
Installation:Category II
StorageTemperature:-20 to +60°C
environmentRelative humidity: 90% RH max.
(no condensation)
Protection classClass I
Power cordUse only the following power supply cord.
Using the wrong power cord could result in
danger or fire. The protection Class I equipment
should be connected to PE (protective earth)
terminal.
• For 220 to 240 VAC area
Approved according to EU/EN standards, 3
conductor grounding Type H05VV-F, 3 m
long maximum, rated at 250 VAC minimum.
22
Page 25
Eyepieces
Table 1
Table 1: Total Magnification and Real Field of Binocular Tube
10 X15 X20 X30 X
Field number 22Field number 16Field number 12.5Field number 7