Nikon SMZ25, SMZ18 Instruction Manual

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Page 1

M608 E 13.5.NF.1

Research Stereo Microscope

SMZ25/SMZ18

*M608EN01*

Instruction Manual

Page 2

Page 3

Introduction

Thank you for purchasing a Nikon product.

This instruction manual is written for users of the Nikon research stereo microscopes for SMZ25 Zooming Body/SMZ18 Zooming Body. To ensure correct usage, read this manual carefully before operating this product.

● No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

● The contents of this manual are subject to change without notice.

● The equipment described in this manual may differ from the actual product in its appearance.

● Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you

note any points that are unclear or incorrect, please contact your nearest Nikon representative.

● Some of the equipment described in this manual may not be included in the set you have purchased.

● If you intend to use any other equipment with this product, read the manual for that equipment too.

● If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be

impaired.

Page 4

Introduction

Contents of the Manual

Chapter 1, “Components” in this instruction manual is presented, based on the system configuration examples of SMZ25 Zooming Body and SMZ18 Zooming Body. Chapter 2, “Microscopy Procedures” describes three different microscopy procedures in accordance with the configuration examples in Chapter 1. Chapter 3, “Operations for Each Purpose” describes which operations are to be performed, followed by Chapter 4, “Functions and Operations of the Devices.”

Chapter 5, “Assembling” lists the components of this microscopy system, considering that the system allows various combination. Devices needed for each illumination method can be identified.

Symbols Used in This Manual

The following symbols are used in this manual.

 Symbols for Safety

WARNING

CAUTION

 Other Symbols

Highlights important information that should be noted for safety. Read “Safety Precautions” for details.

Indicates information you should note or comply with to prevent defects or malfunction of this product.

Indicates information you should be aware of in using this product, as well as other useful information.

Page 5

Contents

Introduction ......................................................................................................................... i

Contents of the Manual .................................................................................................... ii

Symbols Used in This Manual ......................................................................................... ii

Safety Precautions ............................................................................................................ vi

WARNING and CAUTION Symbols ................................................................................ vi

Label Attached to the Product ......................................................................................... vi

WARNING ................................................................................................................ viii

CAUTION ................................................................................................................... x

Notes on Handling the Product ........................................................................................ xii

Components ............................................................................................. 1

1 Microscopy System under Episcopic Illumination .................................................... 2

1.1 Names of the Devices and Operation Sections .................................................. 2

2 Microscopy System under Diascopic Illumination and Epi-fluorescence

Illumination ............................................................................................................... 3

2.1 Names of the Devices and Operation Sections .................................................. 3

2.2 Buttons on the P2-RC Remote Controller .......................................................... 4

3 Compact System ...................................................................................................... 5

3.1 Names of the Devices and Operation Sections .................................................. 5

Microscopy Procedures .......................................................................... 7

1 Operation Flowchart ................................................................................................. 8

2 Procedure for Microscopy under Episcopic Illumination ........................................... 9

3 Procedure for Microscopy under Diascopic Illumination ........................................ 13

4 Procedure for Epi-fluorescence Microscopy ........................................................... 16

Specific Operations ............................................................................... 19

1 Turning On the Power ............................................................................................ 20

2 Adjusting the Brightness ......................................................................................... 21

3 Switching the Optical Path of the Tube .................................................................. 22

4 Adjusting the Angle of Depression ......................................................................... 23

5 Adjusting the Diopter .............................................................................................. 23

6 Adjusting the Interpupillary Distance ...................................................................... 24

7 Focusing on the Sample ......................................................................................... 25

8 Changing the Magnification .................................................................................... 27

8.1 Image Zooming ................................................................................................. 27

8.2 Switching Objectives (Using the P2-RNI2 Intelligent Nosepiece) .................... 28

9 Shifting a Sample ................................................................................................... 29

9.1 Shifting the Stage in the X and Y Directions (P-SXY64 XY Stage) .................. 29

9.2 Using the Sliding Stage ..................................................................................... 29

10 Using the Aperture Diaphragm ............................................................................... 30

11 Using Episcopic Illumination ................................................................................... 30

12 Using Diascopic Illumination .................................................................................. 31

13 Using Epi-Fluorescence Illumination ...................................................................... 32

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Contents

13.1 Switching Filter Cubes ...................................................................................... 32

13.2 Selecting a Filter Cube ..................................................................................... 33

13.3 Important Notes for Epi-Fluorescence Microscopy .......................................... 33

14 Remote Operation .................................................................................................. 33

15 Capturing Images ................................................................................................... 34

15.1 Procedure for Capturing Images ...................................................................... 34

15.2 Tips on Microscope Settings for Photomicroscopy........................................... 35

16 Internally Saved Information ................................................................................... 36

Functions and Operations of the Devices ........................................... 37

1 Zooming Body ........................................................................................................ 38

1.1 SMZ18 Zooming Body ...................................................................................... 38

1.2 SMZ25 Zooming Body ...................................................................................... 39

2 Focus Units............................................................................................................. 41

2.1 P2-FU Focus Unit ............................................................................................. 41

2.2 P2-MFU Motorized Focus Unit ......................................................................... 42

2.3 P2-FMDN Focus Mount .................................................................................... 45

3 Nosepiece .............................................................................................................. 46

3.1 P2-RNI2 Intelligent Nosepiece ......................................................................... 46

4 Objectives ............................................................................................................... 47

5 Bases, Stand, and Stages ...................................................................................... 48

5.1 P2-PB Plain Base ............................................................................................. 48

5.2 P2-DBL LED Diascopic Illumination Base ........................................................ 48

5.3 P2-DBF Fiber Diascopic Illumination Base ...................................................... 48

5.4 P-PS32 Plain Stand .......................................................................................... 49

5.5 P-SXY64 XY Stage ........................................................................................... 50

5.6 C-SSL DIA Sliding Stage .................................................................................. 50

5.7 C-TRS Tilting Stage .......................................................................................... 50

6 Tubes ...................................................................................................................... 51

6.1 P2-TERG 100/ P2-TERG 50 Trinocular Tilting Tube ........................................ 51

6.2 P2-TL100 Trinocular Tube L ............................................................................. 51

7 Episcopic Illuminator .............................................................................................. 52

7.1 P2-CI Coaxial Epi Illuminator ............................................................................ 52

7.2 C-FDF Flexible Double Arm Fiber Illumination Unit .......................................... 53

7.3 P2-FIR Ring Fiber Illumination Unit .................................................................. 53

7.4 P2-FIRL LED Ring Illumination Unit ................................................................. 53

8 Diascopic Illuminator .............................................................................................. 54

8.1 P2-DBL LED Diascopic Illumination Base ........................................................ 54

8.2 P2-DBF Fiber Diascopic Illumination Base ...................................................... 56

8.3 P-DF LED Dark Field Unit................................................................................. 57

8.4 P2-POL Simple Polarizing Attachment ............................................................. 57

9 Epi-Fluorescence Attachment................................................................................. 58

9.1 P2-EFLI Epi Fluorescence Attachment ............................................................. 58

9.2 P2-EFLM Motorized Epi Fluorescence Attachment .......................................... 59

10 Remote Control Device .......................................................................................... 61

10.1 P2-RC Remote Controller ................................................................................. 61

10.2 AZ-FSW Foot Switch ........................................................................................ 63

10.3 AZ-PCR Photo Release .................................................................................... 65

11 Control/Communications Device and Relay Device ............................................... 66

11.1 P2-CTLB Control Box ....................................................................................... 66

11.2 P2-CTLA Control Box ....................................................................................... 68

11.3 P2-RLY Relay Box ............................................................................................ 69

11.4 P2-RLYC Relay Cable ...................................................................................... 69

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Contents

Assembling ............................................................................................ 71

1 System Configuration ............................................................................................. 72

2 Device List .............................................................................................................. 74

3 Assembly Procedures ............................................................................................ 75

3.1 Assembling the EPI System and DIA + EPI-FL System ................................... 75

3.2 Compact System Assembly .............................................................................. 91

3.3 Assembly of Other Devices (Optional) ............................................................. 94

Troubleshooting ..................................................................................... 97

1 Image Viewing ........................................................................................................ 98

2 Operation ................................................................................................................ 98

3 Motorized Devices .................................................................................................. 99

Maintenance and Storage ................................................................... 101

1 Cleaning ............................................................................................................... 102

1.1 Cleaning Lenses ............................................................................................. 102

1.2 Cleaning Parts Other than the Lens ............................................................... 102

1.3 Disinfecting the Product .................................................................................. 103

2 Storage ................................................................................................................. 103

3 Periodic Inspection (Charged) .............................................................................. 103

Specifications ...................................................................................... 105

1 Microscopy (Principles) ........................................................................................ 106

2 Performance Properties ....................................................................................... 106

3 Physical Properties ................................................................................................ 110

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Safety Precautions

To ensure correct and safe operation, read this manual before using this product.

WARNING and CAUTION Symbols

Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using this product. Do not discard this manual and keep it handy for easy reference.

Safety instructions in this manual are marked with the following symbols to indicate their importance. For your safety, always follow the instructions marked with these symbols.

Symbol Description

Disregarding instructions marked with this symbol may lead to serious injury or death.

Disregarding instructions marked with this symbol may lead to injury or property damage.

WARNING

CAUTION

Label Attached to the Product

The label attached to the products shown below indicates that attention should be paid to use of these products. Before using a product with the label attached, be sure to carefully read the manual.

Light safety label

IEC62471 RISK GROUP2 CAUTION

Possibly hazardous optical radiation emitted from this product. Do not stare at operating lamp. May be harmful to the eyes.

Caution: Do not look into the LED light.

Possibly hazardous optical radiation emitted from this product. Do not stare at operating lamp. May be harmful to the eyes. RISK GROUP 2 IEC62471: 2006

Label Position

Light safety label

C-FDF Flexible Double Arm Fiber

Illumination Unit

P2-CI Coaxial Epi Illuminator

Light safety label

P2-FIR Ring Fiber Illumination Unit

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Safety Precautions

Heat warning label

Light leakage warning label

Caution: Heat of the empty cube

Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become hot, resulting in burns. Do not touch the front face of the empty cube. Replace the empty cube several minutes after finishing the diascopic observation.

Label Position

Heat warning label

Caution: Light leakage

Do not use the epi-fluorescence attachment while the filter cube replacement cover is open. Harmful light including ultraviolet light may leak and affect fluorescence observation.

Label Position

PHOT

P2-EFLM Motorized Epi Fluorescence P2-EFLI Epi Fluorescence Attachment Attachment

Light leakage warning label

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Safety Precautions

WARNING

1 Do not disassemble.

Disassembling this product may result in electric shock or malfunction. Malfunction and damage due to disassembling or modification are unwarranted.

Do not disassemble parts other than those described in this manual. If you experience problems with this product, contact your nearest Nikon representative.

2 Read the instruction manuals carefully.

To ensure safety, carefully read this manual and the manuals for other equipment to be used with this product. Particularly, all warnings and cautions given at the beginning of each manual must be observed.

Safety is a top design priority for Nikon products. Safety is ensured as long as the user observes all of the warnings and cautions given in the manuals, and uses the system only for its intended purpose. However, failure to heed the warnings and cautions given in the manuals, subjecting the system to shock or impact, or attempting to disassemble the system may result in unexpected accidents and injury.

Product with an epi-fluorescence attachment:

The light source used for Epi-fluorescence microscopy (HG Precentered Fiber Illuminator) requires special care during handling because of its characteristics. Be sure to refer to the manual for the light source being used.

5 Heat from the illuminator

During and immediately after a period of illumination, the lamp and surrounding areas (including the lamp house) are very hot if the light source of the illumination device is a mercury lamp.

● Do not touch the lamp or surrounding areas during or immediately after a period of illumination. There is a risk of burn if you touch the hot area.

● Always attach the lamp house cover when using this product.

● Make sure the lamp and surrounding areas have cooled sufficiently (which may take approximately 30 minutes) before attempting to replace the lamp.

● Do not place fabric, paper, or highly flammable volatile materials (i.e. gasoline, petroleum benzene, paint thinner, and alcohol) near the lamp house while the lamp is on or for about thirty minutes after it was turned off. Because the lamp is very hot, ignition or fire may be induced.

3 Checking the input rating

For a device which uses a power supply, check that the input rating of the device matches the power supply to be used. If the input rating does not match, do not use the power supply and contact your local representative. Using the device with the power supply can cause the device to be damaged.

4 Notes on the power cord

Be sure to use the specified power cord. Use of other power cords may result in malfunction or fire. This product is classified as having Class I protection against electric shock. Make sure this product is connected to an appropriate protective earth terminal.

See Chapter 8, “2 Performance Properties” for the specified power cords.

● To prevent electric shock, always turn off the power switch (Press to the “O” position.) for the device before connecting or disconnecting the power cord.

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Safety Precautions

WARNING

6 Hazards of mercury lamps

(when using the epi-fluorescence attachment)

The light source used with the epi-fluorescence attachment (HG Precentered Fiber Illuminator) requires special care during handling because of its characteristics. For safe and correct use of this system, carefully read the warnings below. Keep in mind all potential hazards. Additionally, carefully read the manual for the illuminator and the manual from the lamp manufacturer (if provided), then follow the instructions given therein. Failure to heed the warnings and cautions given in the manuals, subjecting the system to shock or impact, or attempting to disassemble the system may result in unexpected accidents and injury.

● Ultraviolet light

When lit, mercury lamps radiate ultraviolet light

that can damage the eyes and skin. Do not directly look at the light of the lamp. Doing so may cause your eyesight to be lost.

When changing filter cubes, always turn off the

light source. Leaving the lamp turned on during filter cube replacement may result in ultraviolet exposure.

● High-pressure gas

The lamps contain sealed gas under very high

pressure. And the pressure increases when the lamp is on. Crack or dirt of the lamp tube, strong external force or impact, or use beyond the tube’s life may cause the encapsulated gas to leak or the lamp to blow up. If this happens, an accident such as gas inhalation or injury with glass may take place.

● Heat

When the lamp is lit, the lamp and surroundings

will become extremely hot. Do not touch the lamp with bare hands or place flammable materials near the lamp. Failure to comply may result in burns or fire.

7 Hazardous sample handling

This product can also be used for microscopy and imaging of biological samples (cells/tissues) in a petri dish.

Check to determine whether a sample is hazardous before handling. If the sample is hazardous, handle it in accordance with the standard procedure specified for your laboratory. If the sample is potentially infectious, wear rubber gloves and avoid direct contact with the samples. If such a sample is spilled onto this product, the portion must be decontaminated in a safety manner. Consult your safety supervisor or safety standard of your facility.

● Designated lamp

Be sure to use the designated lamp. Using other

types of lamps may result in accidents, including bursting of the lamp.

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Safety Precautions

CAUTION

1 Power shutdown

To prevent electric shock and/or malfunction, always turn off the power switch(es) for this product and the peripheral devices (press to the “O” position) and unplug the power cord from the wall outlet before assembling this product, connecting or disconnecting cables, replacing lamps, or cleaning the zooming body and the objective.

2 Do not look into the LED light

The P2-CI Coaxial Epi Illuminator, C-FDF Flexible Double Arm Fiber Illumination Unit and P2-FIR Ring Fiber Illumination Unit use LEDs as the light source. A label indicating light safety is attached to these devices in order to pay attention to the following. (For details about the label attachment position, see “Label Attached to the Product” on page vi.)

“Possibly hazardous optical radiation emitted from this product. Do not stare at operating lamp. May be harmful to the eyes. RISK GROUP 2 IEC62471: 2006”

3 Be careful of the heat of the empty cube

4 Caution on light leakage

Do not use the epi-fluorescence attachment while the filter cube replacement cover is open. Harmful light including ultraviolet light may leak and affect fluorescence observation.

5 Specification of a light source

Be sure to use the specified light source for an illumination device. Using a light source not specified can cause malfunction and induce an accident. For the specified light source, see Chapter 8, “2 Performance Properties” or the instruction manual for the light source.

6 Movement of motorized device

The microscope system contains motorized devices. To avoid accidental injury by the movements of the motorized devices, be careful with the following when controlling the movements of motorized device with remote controls or personal computers.

● Before operation, check the state of the entire microscope system to ensure safety when operating the motorized devices.

● If having touched the zooming body, focus knob, objective, stage, or devices or samples placed on the stage, your hand or fingers may be caught and injured. Before starting to operate, keep your hands away from these items.

7 Avoid contact with water or chemical solutions.

Never expose this product to water or chemical solutions, and avoid using this product in circumstances where there is risk of exposure to water or chemical solutions. Exposure of electric parts (such as the HG Precentered Fiber Illuminator) to liquids may cause a short circuit, resulting in malfunction or abnormal heating. If water or a chemical solution is splashed onto a device, immediately turn off the power switch for the device (press to the “O” position) and remove the power cord from the receptacle. Then wipe off moisture with a piece of dry cloth or something similar. If water or a chemical solution enters this product, stop using the product, and contact your nearest Nikon representative.

8 Remove any covers from the product before

switching on.

Do not use a device while it is covered with a piece of cloth, etc., Especially for a light source, which is heated, the disturbance of heat release may cause abnormal heat generation, inducing ignition or fire. Do not cover the device with a piece of cloth or similar while in use.

9 Notes on laying cables

Connect and route the cables in accordance with the specified methods. If not connecting and routing the cables properly, the devices may not function correctly.

10 Do not place any object on top of the product.

Do not place any object on top of this product.

11 Cautions on assembling and installing the

product

● Take care to avoid pinching your fingers or hands during product assembly and installation.

● Scratches or fouling optical components (such as lens and filters) with fingerprints, etc. will degrade microscope images. Be careful to avoid scratches or direct contact with the lens and filters when assembling.

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Safety Precautions

CAUTION

● The total weight of the microscope system is ove 20 kg. When moving the system to another location, separate the removable devices temporarily and carry the system while firmly holding the hand holding portions of the base, with multiple persons. Do not lift the system while holding the binocular part of the tube or putting hands under the objective.

● Do not place this product in a locker or cabinet.

12 Cautions on sustained observations

To relieve fatigue resulting from long observation sessions, limit continuous observations to one hour. Take at least 10 to 15-minute breaks between observation sessions. Adjust the layout of other equipment used and the height of your chair.

13 Cautions on use, transportation, and storage

This product must be operated, transported, or stored in accordance with the following conditions. If you install the product at a location with high temperature and high humidity, mold and/or condensation may be generated on the lenses. Furthermore, performance deterioration and malfunction may be resulted.

● Operating conditions:

temperature: 0 to +40C, humidity: 60% RH max. (no condensation)

● Transporting/storage conditions:

temperature: -20 to +60C, humidity: 90% RH max. (no condensation)

14 Cautions on the disposal of the product

When disposing of this product, follow the rules of the facility.

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Notes on Handling the Product

1 Handle the product carefully.

This product is a precision instrument. Avoid subjecting it to sudden impacts and shocks.

Even relatively minor impacts are capable of affecting the precision of the objective.

2 Scratches, dirt, and foreign particles on the lens

Scratches or fouling optical components (such as lens and filters) with fingerprints, etc. will degrade microscope images. If these parts become dirty, clean them as described in Chapter 7, “1.1 Cleaning Lenses.”

3 Installation location

This product is a precision instrument. Usage or storage of this product in an inappropriate environment may result in malfunction or a degradation in precision. Consider the following factors when selecting an installation location:

● Select a location free of vibration. Install this product on a level surface.

● Install this product at least 10 cm away from walls.

● Select a location less exposed to hazards in the

event of collisions, earthquakes, or other potential disasters. To keep this product from falling, use a strong rope or other means if necessary to secure it to the working desk or other heavy, stable item.

● Select a layout that allows easy removal of the power cord from the device’s AC inlet in the event of an emergency.

● Do not use a desk mat or similar.

● Avoid locations exposed to direct sunlight,

locations immediately under room lights, and other bright locations.

● Light from room lights just above this product may enter the objective as extraneous light. If possible, switch off the room lights directly above this product when making observations.

● Select a location with minimal dust.

● To avoid splashes, do not use this product near

water.

● Make sure the ambient temperature is 0 to + 40C and humidity is 60% or less. When transporting or storing this product, the ambient temperature must be -20 to +60C, with the humidity at 90% RH max (with no condensation). If you install the product at a location with high temperature and high humidity, mold and/or condensation may be generated on the lenses. Furthermore, performance deterioration and malfunction may be resulted.

● Do not place this product in a locker or cabinet.

Notes on Handling the Product

4 Handling a focus knob

● Never turn the focus knobs in opposite directions at the same time. Doing so may damage the product.

5 Protect the ports from dust and extraneous light

If not using the vertical tube of the trinocular tube, be sure to attach the supplied cap. Otherwise, extraneous light and dust may be trapped inside the product.

6 Handling of filters (when using the

epi-fluorescence attachment)

● Excitation filters inside a filter cube are exposed to strong light and degrade over time. Replace them after the appropriate number of hours of use.

● Filter characteristics may alter if the filter is exposed to high humidity. To prevent changes or degradation of filter characteristics, avoid using or storing the filters under conditions of high humidity or high temperature. Avoid subjecting filters to rapid temperature changes. When a filter is not in use, store in a desiccator or hermetically sealed container with a drying agent.

● Especially the filters in GFP type filter cubes offer sharp, high-resolution waveform characteristics superior to normal filters. However, due to their sophisticated coatings, they must be handled with special care. Be sure to handle the filters carefully. Take care to avoid abrasion from cleaning. Follow the description in Chapter 7, “1.1 Cleaning Lenses.”

7 Motorized devices

When using motorized devices, do not force the motorized devices to move/stop by hand.

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Chapter

Components

In the stereo microscopes SMZ25 and SMZ18, the SMZ25 Zooming Body or SMZ18 Zooming Body is attached to a motorized or manual focus mount. In addition, arbitrary models can be selected and combined from such device models as bases, objectives, tubes and episcopic illuminators.

This chapter introduces the names of the devices and operation sections with the following three types of system configuration as examples.

1 Microscopy system under episcopic illumination with an SMZ18 Zooming Body

2 Microscopy system under diascopic illumination and epi-fluorescence illumination with an SMZ25 Zooming Body

3 Compact system with an SMZ18 Zooming Body

For details about the overall system configuration, see Chapter 5, “1 System Configuration.”

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1.1

Trinocular Tiltin

Chapter 1 Components

Microscopy System under Episcopic Illumination

Names of the Devices and Operation Sections

P2-TERG 100

Tube

DS camera head

Optical path switching leve

P2-FU Focus Unit

P2-CI Coaxial Epi

Illuminato

Zooming knob

SMZ18 Zooming Bod

P2-CTLB Control Bo

P2-PB Plain Base

Focus knob torque

adjustment ring

P2-RLY Relay Box

(To P2-CTLB Control Box)

P2-RNI2 Intelligent Nosepiece

Coarse focus knob

Fine focus knob

C-FLED2 LED Light Source for Fiber Illuminator (See the instruction manual for the LED light source for fiber illuminator.)

Diopter adjustment ring

Eyepiece

(to C-FLED2 LED Ligh

Fibe

Source for Fibe

Illuminator)

Coarse focus knob

Fine focus knob

u 0

im C x

v a

M W

Zooming knob

perture diaphragm dial

Nosepiece

Fiber (from the P2-CI Coaxial Epi Illuminator)

Objective

Power switch

Stage plate

For the names of the components needed for assembly and connections such as the fixing screws and connectors, see Chapter 5, “3 Assembly Procedures.”

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2.1

P2-TERG 100

Trinocular Tilting Tube

Chapter 1 Components

Microscopy System under Diascopic Illumination and Epi-fluorescence Illumination

Names of the Devices and Operation Sections

DS camera head

Optical path switching lever

P2-MFU Motorized Focus Unit

Turret address check window

P2-EFLM Motorized

Epi Fluorescence

Attachmen

SMZ25 Zooming Bod

P2-CTLA Control Bo

Light shielding plate

P2-DBL LED Diascopic

Illumination Base

LED power source

ON/OFF switch

LED brightness control

dial

HG fibe

(to the HG precentered

fiber illuminator)

OCC adjustmen

knob

P2-RNI2 Intelligent Nosepiece

P2-RC Remote Controller (See “2.2 Buttons on the P2-RC Remote Controller.”)

C-HGFIE HG precentered fiber illuminator (See the instruction manual for the HG precentered fiber illuminator.)

Prism sheet IN/OUT lever

Diopter adjustment ring

Eyepiece

perture diaphragm dial

STOP switch

Power switch

AC adapte

input terminal

Objective

LED brightness control terminal

LED brightness control selection switch

Nosepiece

Stage plate

For the names of the components needed for assembly and connections such as the fixing screws and connectors, see Chapter 5, “3 Assembly Procedures.”

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2.2

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine

0.5x

0.1x

(6) Filter cube switching

(4) Fine/coarse focus

switching button

(3) Focus knob

Chapter 1 Components

Buttons on the P2-RC Remote Controller

(1) LCD

button

FL Cube

BRIGHTNESS

(9) Capture button

(2) Backlight control dial

(4) Fine/coarse focus

switching button

(8) Z-reset button

(3) Focus knob

(7) LCD display switching

(5) Focus speed indicato

(13) Escape button

(14) Escape ON/OFF

(17) ALZ button

(18) ALZ ON/OFF

(19) Diascopic LED

brightness control button

button

indicato

Coarse

DISPLAY

Z-SPEED

ESCAPE

ON/OFF

ALZ

ON/OFF

Fine

Ex Fine

−+

−

ZOOM STEP

1x1x0.5x

Z-RESET

LIMIT

ON/OFF

(10) Zoom button

(12) Zoom step indicato

0.1x

(11) Zoom step switching

button

(15) Limit button

(16) Limit ON/OFF indicato

(20) Diascopic LED ON/OFF

button

(21) Diascopic LED ON/OFF

indicator

(2) Backlight control dial

(4) Fine/coarse focus

switching button (on both sides)

Front Right Side

For details about the buttons’ functions, see “Functions and indications of the operation buttons” in Chapter 4, “10.1 P2-RC Remote Controller.”

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3.1

P2-TL100 Trinocular Tube

SMZ18 Zooming Bod

Chapter 1 Components

Compact System

Names of the Devices and Operation Sections

DS camera head

Optical path switching leve

Zooming knob

P2-FMDN Focus Mount

Coarse focus knob

Fine focus knob

Objective

P-PS32 Plain Stand

Clamp tool

Zooming knob

Focus knob torque

adjustment ring

Coarse focus knob

Fine focus knob

Diopter adjustment ring

Eyepiece

perture diaphragm dial

Stage plate

For the names of the components needed for assembly and connections such as the fixing screws and connectors, see Chapter 5, “3 Assembly Procedures.”

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Chapter 1 Components

☆☆☆

Page 21

Chapter

Microscopy Procedures

This chapter describes the microscopy procedures listed below.

The devices used in the descriptions are based on the configuration examples given in the previous chapter.

Procedure 1: Procedure for microscopy under episcopic illumination

Procedure 2: Procedure for microscopy under diascopic illumination

Procedure 3: Procedure for epi-fluorescence microscopy

It is assumed that all required components are attached to the microscope with all necessary cables properly connected.

If assembly of the microscopy system has not yet been completed, complete assembly as described in Chapter 5, “3 Assembly Procedures.”

When using the motorized system or intelligent system for microscopy, see Chapter 3, “16 Internally Saved Information.”

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Chapter 2 Microscopy Procedures

Microscopy under episcopic illumination or

Turn on the power.

Set a sample.

Bring an objective into the optical

path.

Turn on the illumination and adjust

the brightness.

Set the optical path to 100% to the

binocular part.

Adjust the depression angle of the

tilting tube.

Adjust the diopter.

Adjust the interpupillary.

Operation Flowchart

diascopic illumination

Adjust the focus.

Change the magnification using the

zooming knobs.

Turn off the power after completing

microscopy.

* In microscopy under diascopic

illumination using an epi-fluorescence attachment, attach an empty cube (supplied with the microscope) to the epi-fluorescence attachment and bring it into the optical path.

* In a compact system of an SMZ18

Zooming Body combined with a P-PS32 Plain Stand and P2-FMDN Focus Mount, perform the steps above in the order of [2], [5], [6], [7], [8], [9], and [10].

Epi-fluorescence microscopy

After locating the target in the sample with microscopy under diascopic illumination, follow the procedure below:

Turn off the diascopic LED illumination.

Bring the filter cube into the optical path.

Adjust the angle of the light shielding plate.

Turn on the HG precentered fiber illuminator and adjust the brightness.

Adjust the focus.

Change the magnification by zooming in and out.

Turn off the power after completing microscopy.

Page 23

Chapter 2 Microscopy Procedures

This section describes the procedure for performing microscopy under episcopic illumination using the SMZ18 Zooming Body combined with the P2-CI Coaxial Epi Illuminator.

Procedure for Microscopy under Episcopic Illumination

Turn on the power.

Turn on the P2-CTLB Control Box by pressing the power switch to the “|” position. Check that the power LED on the control box lights.

Set a sample.

Place a sample on the stage plate and immediately under the objective.

Power supply to the DS-L3 DS Camera

Control Unit

When the DS-L3 DS Camera Control Unit is connected, turn on the control box and then the DS-L3.

Sample

Power LED: On

Power switch: “|” position

Power on

Bring an objective into the optical path.

Turn the nosepiece to bring the objective to be used into the optical path. Check that the objective falls into the click position when turning the nosepiece. (See Chapter 3, “8.2 Switching Objectives.”)

P2-CI Coaxial Epi Illuminator

 The P2-CI Coaxial Epi Illuminator is supplied

with a 1/4 lambda plate. When using the 0.5x or 1x objective, attach the 1/4 lambda plate to the tip of the objective. Turn the 1/4 lambda plate so that the white circle (indicator) is at the front.

 For the P2-CI Coaxial Epi Illuminator, available

objectives and zooming ratios are restricted. For details, see Chapter 4, “7.1 P2-CI Coaxial Epi Illuminator.”

Setting the sample

Rotating the nosepiece

Objective on the optical path

Page 24

Chapter 2 Microscopy Procedures

Turn on the illumination and adjust the brightness.

Refer to the instruction manual for the C-FLED2 LED Light Source for Fiber Illuminator.

Set the optical path to 100% to the binocular part.

Pull out the optical path switching lever of the tube to set the optical path to 100% to the binocular part.

Aperture diaphragm

The SMZ18 Zooming Body is equipped with an aperture diaphragm. Turn the aperture diaphragm dial counterclockwise (Ο side) to fully open the aperture. (For details about using the aperture diaphragm, see Chapter 3, “10 Using the Aperture Diaphragm.”)

Moving the optical path switching lever

Pulling out the optical path switching lever: 100% to the binocular part Pushing in the optical path switching lever: 100% to the vertical tube

The division ratio of the light amount differs depending on the tube types. (See Chapter 3, “3 Switching the Optical Path of the Tube.”)

Optical path to the binocular part: 100%

Adjust the depression angle of the tilting tube.

When using the P2-TERG 100 or P2-TERG 50 Trinocular Tilting Tube, change and adjust the angle of the binocular part to facilitate microscopy. (The low eye level P2-TL100 Trinocular Tube L does not have a mechanism for adjusting the angle of depression.)

Notes on adjusting the angle of depression

 Do not apply excessive force to the binocular part during depression angle adjustment. It may cause unexpected

failure or malfunction.

 When moving the binocular part, be careful not to catch your fingers in the rotary section at the bottom of the

binocular part.

Adjust the diopter.

djust to match your diopter. For details, see Chapter 3, “5 djusting the Diopter.”

This adjustment is required for each user.

Adjusting the angle of depression

Adjusting the diopter

Page 25

Chapter 2 Microscopy Procedures

Adjust the interpupillary.

djust to match the distance between your right and left eyes. Look into both eyepieces and move the binocular part of the tube so that the right and left fields of view merge into a single circle. This adjustment is required for each user.

Adjust the focus.

Turn the focus knobs (coarse/fine control) on the right and left of the focus unit to move the focus mount of the focus unit vertically and focus on the sample. The figure shows the turning directions of a knob and the corresponding vertical movements of the focus mount (zooming body).

 Turning the knob backward: Upward movement  Turning the knob forward: Downward movement

(See Chapter 3, “7 Focusing on the Sample.”)

djusting the interpupillary distance

Upward movement

Downward movement

Focusing

Change the magnification using the zooming knobs.

Turn the zooming knobs on the right and left of the SMZ18 Zooming Body to change the magnification. The figure shows the turning directions of a knob and the corresponding changes in magnification.

 Turning the knob backward: Magnification increase  Turning the knob forward: Magnification decrease

The magnification continuously changes from 0.75x to

13.5x. Zoom magnification values are marked on the zooming knobs.

Click stop during image zooming

It is also possible to cause a click stop to occur at predetermined magnification positions during image zooming. (See Chapter 4, “1.1 SMZ18 Zooming Body.”)

P2-CI Coaxial Epi Illuminator

For the P2-CI Coaxial Epi Illuminator, available objectives and zooming ratios are restricted. For details, see Chapter 4, “7.1 P2-CI Coaxial Epi Illuminator.”

Zoom operation

Magnification increase

Magnification decrease

Page 26

Chapter 2 Microscopy Procedures

Turn off the power after completing microscopy.

Turn off the P2-CTLB Control Box by pressing the power switch to the “O” position. Check that the power LED on the control box turns off.

lso turn off the LED light source for fiber illuminator.

Power LED: Off

Power switch: “O” position

Power off

Page 27

Chapter 2 Microscopy Procedures

This section describes the procedure for microscopy under diascopic illumination using the SMZ25 Zooming Body combined with the P2-DBL LED Diascopic Illumination Base.

For the same operations as in “2 Procedure for Microscopy under Episcopic Illumination,” details are omitted.

Procedure for Microscopy under Diascopic Illumination

Turn on the power.

Turn on the P2-CTLA Control Box by pressing the power switch to the “|” position. Check that the power LED on the control box lights.

P2-MFU Motorized Focus Unit

When the power is turned on, the motorized focus unit is initialized as follows:

(1) The focus mount rises to the upper sensor

limit.

(2) This is followed by downward movement to the

position when power was turned on.

* Other motorized devices also perform internal

initialization.

Power supply to the P2-DBL LED Diascopic Illumination Base

For a system configuration where a control box has been connected, the control box supplies power to the P2-DBL LED Diascopic Illumination Base. The P2-DBL therefore need not be connected to a power supply. (See Chapter 4, “8.1 P2-DBL LED Diascopic Illumination Base.”)

Power supply to the DS-L3 DS Camera

Control Unit

When the DS-L3 DS Camera Control Unit is connected, turn on the control box and then the DS-L3.

Power on

Power LED: On

Power switch: “|” position

Set a sample.

→ Step 2 in “2 Procedure for Microscopy Under Episcopic Illumination”

→ Step 3 in “2 Procedure for Microscopy Under Episcopic Illumination”

Empty cube

When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical path.

Bring an objective into the optical path.

Page 28

Chapter 2 Microscopy Procedures

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

Turn on the illumination and adjust the brightness.

Check that the brightness control selection switch on the

LED diascopic illumination base is set to the ON position. Press the diascopic LED ON/OFF button on the remote controller to turn on the LED illumination. Check that the LED ON/OFF indicator lights. Press the diascopic LED brightness control buttons to adjust the brightness. Press [+] to increase the brightness and [-] to decrease the brightness.

Illumination on and brightness control rights

To turn on the diascopic illumination and perform brightness control directly with the LED diascopic illumination base, set the LED brightness control selection switch to the OFF position.

For details about using the diascopic illumination base, see Chapter 4, “8.1 P2-DBL LED Diascopic Illumination Base.”

Aperture diaphragm

The SMZ25 Zooming Body is equipped with an aperture diaphragm. Turn the aperture diaphragm dial counterclockwise (O side) to fully open the aperture. (For details about using the aperture diaphragm, see Chapter 3, “10 Using the Aperture Diaphragm.”)

TRL

LED brightness control selection switch

Diascopic LED brightness control button

CTRL

ON OFF

Setting brightness control right

to the control box

Coarse

DISPLAY

Z-SPEED

ESCAPE

ON/OFF

ALZ

ON/OFF

Fine

Ex Fine

FL Cube

−

ZOOM STEP

−+

ZOOM STEP

Z-RESET

0.5x

BRIGHTNESS

0.1x

LIMIT

ON/OFF

Remote on/brightness control

Diascopic LED ON/OFF button

LED ON/OFF indicato

Set the optical path to 100% to the binocular part.

→ Step 5 in “2 Procedure for Microscopy Under Episcopic Illumination”

Adjust the depression angle of the tilting tube.

→ Step 6 in “2 Procedure for Microscopy Under Episcopic Illumination”

Adjust the diopter.

→ Step 7 in “2 Procedure for Microscopy Under Episcopic Illumination”

Adjust the interpupillary.

→ Step 8 in “2 Procedure for Microscopy Under Episcopic Illumination”

Page 29

Chapter 2 Microscopy Procedures

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

Adjust the focus.

Turn the focus knobs of the remote controller to move the focus mount of the focus unit vertically and focus on the sample. The figure shows the turning directions of a knob and the corresponding vertical movements of the focus mount (zooming body).

 Turning the knob backward: Upward movement  Turning the knob forward: Downward movement

(See Chapter 3, “7 Focusing on the Sample.”)

Focus speed switching

The focus speed can be switched between coarse, fine, and extra fine control.

Use the fine/coarse focus switching button on the remote controller to switch the speed.

Each time the button is pressed, the focus speed is cyclically switched in the order of coarse, fine, and extra fine.

When the focus speed is changed, the corresponding indicator (Coarse/Fine/ExFine) lights.

 Coarse: 2 to 10 mm/second  Fine: 0.2 to 5 mm/second

Focus speed indicator

 Extra fine: 0.01 to 0.1 mm/second

Upward movement

Downward movemen

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

ESCAPE

ON/OFF

ALZ

ON/OFF

Ex Fine

−

ZOOM STEP

−+

ZOOM STEP

Z-RESET

0.5x

Focus operation

Fine/coarse focus switching button

BRIGHTNESS

0.1x

LIMIT

ON/OFF

Change the magnification by zooming in and out.

Press the zoom buttons on the remote controller to change the magnification of the zooming body.

 Upper zoom button: Magnification increase  Lower zoom button: Magnification decrease

The magnification changes in the range from 0.63x to

Upper zoom button (magnification increase)

15.75x. Pressing and holding the buttons causes the magnification to change continuously.

Magnification step size

Each time the zoom step switching button is pressed, the step size changes in the following order: 1x, 0.5x

Lower zoom button (magnification decrease)

then 0.1x. When a step size is selected, the corresponding step LED indicator lights. (See Chapter 4, “1.2 SMZ25 Zooming Body.”)

Turn off the power after completing microscopy.

Turn off the P2-CTLA Control Box by pressing the power switch to the “O” position. Check that the power LED on the control box turns off.

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

ESCAPE

ON/OFF

ALZ

ON/OFF

Ex Fine

−

ZOOM STEP

−+

ZOOM STEP

Z-RESET

0.5x

0.1x

LIMIT

ON/OFF

Zoom operation

BRIGHTNESS

Zoom step indicator

Zoom step switching button

Power LED: Off

Power switch: “O” position

Power off

Page 30

Chapter 2 Microscopy Procedures

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

After locating a target in a sample in “3 Procedure for Microscopy Under Diascopic Illumination” (steps 1 to 10), follow the procedure below:

Procedure for Epi-fluorescence Microscopy

Turn off the diascopic LED illumination.

Press the diascopic LED ON/OFF button on the remote controller to turn off the LED illumination. Check that the diascopic LED ON/OFF indicator turns off.

Bring the filter cube into the optical path.

Press the filter cube switching buttons (FL1 to FL4) on the

remote controller to bring the desired filter cube into the optical path. FL1 to FL4 designate the turret addresses of the epi fluorescence attachment. The LED of the pressed button lights so that the turret address on the optical path can be identified. The address on the optical path can be identified also through the turret address check window of the epi fluorescence attachment.

ZOOM STEP

0.5x

Z-RESET

BRIGHTNESS

0.1x

LIMIT

ON/OFF

Diascopic LED ON/OFF button

Diascopic LED ON/OFF indicato

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

Ex Fine

−

ZOOM

ESCAPE

STEP

ON/OFF

ALZ

−+

ON/OFF

Diascopic LED illumination off

Filter cube switching button

ZOOM STEP

Z-RESET

0.5x

BRIGHTNESS

0.1x

LIMIT

ON/OFF

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

Ex Fine

−

ZOOM

ESCAPE

STEP

ON/OFF

ALZ

−+

ON/OFF

Turret rotation in the epi fluorescence attachment

Bringing the filter cube into the optical path

Turret address check window

Page 31

Chapter 2 Microscopy Procedures

Adjust the angle of the light shielding plate.

Change the angle of the light shielding plate to 45° to

prevent light reflected from the objective from entering your eyes.

Turn on the HG precentered fiber illuminator and adjust the brightness.

Refer to the instruction manual for the fiber light source.

Adjust the focus.

→ Step 9 in “3 Procedure for Microscopy Under Diascopic Illumination”

Adjusting the angle of the light shielding plate

Change the magnification by zooming in and out.

→ Step 10 in “3 Procedure for Microscopy Under Diascopic Illumination”

Turn off the power after completing microscopy.

Turn off the HG precentered fiber illuminator and P2-CTL Control Box by pressing their power switches to the “O” position. Check that their power LEDs go off.

Power off

Power LED: Off

Power switch: “O” position

Page 32

Chapter 2 Microscopy Procedures

☆☆☆

Page 33

Chapter

Specific Operations

This chapter describes how to work with this microscope system in order to observe samples.

For details about using the individual devices, see Chapter 4.

Page 34

Chapter 3 Specific Operations

When using this microscope system, power to the following devices must be turned on:

 P2-CTLA Control Box  P2-CTLB Control Box  P2-DBL LED Diascopic Illumination Base

 P2-FIRL LED Ring Illumination Unit (See the instruction manual for the P2-FIRL LED Ring Illumination Unit.)  P-DF LED Dark Field Unit (See the instruction manual for the P-DF LED Dark Field Unit.)

When using an illuminator whose light source is an HG precentered fiber illuminator or fiber illumination LED light source, also turn on the power to the light source. (See the instruction manual for the light source.)

When using multiple power supplies, they need not be turned on in a particular order except for the following cases:

 When using a DS camera control unit to capture images, power on the DS camera control unit at the end.  When controlling a C-HGFIE HG Precentered Fiber Illuminator from NIS-Elements, etc. via a control box, power on the

HG precentered fiber illuminator before the control box.

Press the power switch to the “|” position to turn the power on and to the “O” position to turn the power off.

When power is turned on, the power LED lights.

Turning On the Power

12 V AC adapter

For a system configuration where a control box has been connected, the P2-DBL LED Diascopic Illumination Base need not be connected to a power supply. The control box supplies the power. If a control box is not used, connect the 12 V AC adapter to the P2-DBL.

LED power On/Off control right

If the LED brightness control selection switch on the base has been set to ON to assign the control right to the control box, the LED power On/Off switch and LED brightness control dial on the base need not be used. They can be remotely controlled using the remote controller connected to the control box or a personal computer (software: NIS-Elements).

Power LED (Lit: ON)

Power switch (“|” to turn on; “O” to turn off)

Power switch of the control box

LED power On/Off switch (“|” to turn on; “O” to turn off)

Power switch of the P2-DBL LED Diascopic Illumination Base

Page 35

Chapter 3 Specific Operations

The illumination brightness can be adjusted for the following devices. Turn the brightness control dial to adjust the brightness to a comfortable level.

Depending on the system configuration, some devices support remote control of the brightness.

For details, see the sections in Chapter 4 describing how to use the individual devices.

 Adjust the brightness using the adjustment volume of the

device  P2-DBL LED Diascopic Illumination Base

 Adjust the brightness using the C-FLED2 LED Light source fo

fiber illuminator (See the instruction manual supplied with the C-FLED2 for details.)

 P2-CI Coaxial Epi Illuminator  C-FDF Flexible Double Arm Fiber Illumination Unit  P2-FIR Ring Fiber Illumination Unit  P2-DBF Fiber Diascopic Illumination Base

Adjusting the Brightness

Adjusting the diascopic illumination brightness

 Adjust the brightness using the C-HGFI/C-HGFIE HG

Precentered Fiber Illuminator (See the instruction manual supplied with the C-HGFI/C-HGFIE for details.)

 P2-EFLI Epi Fluorescence Attachment  P2-EFLM Motorized Epi Fluorescence Attachment

 Adjust the brightness using the power supply section of each

unit (See the instruction manual supplied with the P2-FIRL/P-DF for details.)

 P2-FIRL LED Ring Illumination Unit  P-DF LED Dark Field Unit

Page 36

Chapter 3 Specific Operations

For observing a specimen using the eyepiece, switch the optical path to the binocular part. For imaging using the DS camera head, switch the optical path to the vertical tube. Use the optical path switching lever of the tube to switch the optical path. Push or pull the lever to make the desired selection. Be sure the lever is fully pushed in or pulled out. Do not leave the lever stopped partway.

Switching the Optical Path of the Tube

Distribution of Light with Optical Path Switching (%)

Switching the optical path

Tube Lever position

P2-TERG 100 Trinocular Tilting Tube

P2-TERG 50 Trinocular Tilting Tube Pulled out 100 100 0

P2-TL100 Trinocular Tube L Pulled out 100 100 0

* The light amount of the left eye is always 100%. The light from the optical path of the right eye is diverged to the vertical tube.

(Images of the right eye’s optical path are captured.)

Pulled out 100 100 0

Pushed in 100 0 100

Pushed in 100 50 50

Pushed in 100 0 100

Binocular part

Vertical tube*

Left Right

Page 37

Chapter 3 Specific Operations

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

When the P2-TERG 100 Trinocular Tilting Tube or P2-TERG 50 is used, the angle of the binocular part to facilitate microscopy can be changed and adjusted.

The angle can be continuously changed by moving the binocular part as indicated by the arrow in the figure.

The eye level can also be changed by turning the binocular part 180°.

(The low eye level P2-TL100 Trinocular Tube L does not have a mechanism for adjusting the angle of depression.)

Notes on adjusting the angle of depression

 Do not apply excessive force to the binocular part during depression angle adjustment. It may cause unexpected failure or

malfunction.

 When moving the binocular part, be careful not to catch your fingers in the rotary section at the bottom of the binocular part.

Adjusting the Angle of Depression

Adjusting the angle of depression

Adjusting the Diopter

Adjust to match your diopter. This adjustment is required for each user.

1 Position the diopter adjustment rings of the right and left

eyepieces to 0.

2 Maximize the magnification, and then focus on the sample

using the focus knob. (See “7 Focusing on the Sample.”)

3 Minimize the magnification, and then turn the diopter

adjustment rings of the right and left eyepieces to bring them into focus separately.

[Image zooming]

SMZ18 Zooming Body: Zooming knob on the zooming body

SMZ25 Zooming Body: Zoom buttons on the remote

controller or the right pedal of the foot switch

[Zoom magnification]

SMZ18 Zooming Body: 0.75x to 13.5x

SMZ25 Zooming Body: 0.63x to 15.75x

4 Repeat steps 2 and 3 to check the focus, so that changing

the magnification does not affect the focus and clear images can be captured.

Magnification decrease

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

ESCAPE

ON/OFF

ALZ

ON/OFF

Ex Fine

−

ZOOM STEP

−+

ZOOM STEP

Z-RESET

0.5x

0.1x

LIMIT

ON/OFF

Remote controller

Magnification increase

BRIGHTNESS

Magnification increase

Magnification decrease

Magnification increase

Magnification

Foot switch

decrease

Adjusting the diopter

Page 38

Chapter 3 Specific Operations

djust to match the distance between your right and left eyes.

This adjustment is required for each user.

Look into both eyepieces and move the binocular part of the tube so that the right and left fields of view merge into a single circle.

Adjusting the Interpupillary Distance

Adjusting the interpupillary distance

Page 39

Chapter 3 Specific Operations

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

Focus adjustment of the sample is performed by the rotation of the focus knobs on the focus unit or focus mount. When the remote controller or foot switch is connected, focusing can be adjusted using the focus knobs of the remote controller or the foot pedals on the left side of the foot switch. To use the foot switch, the P2-RC Remote Controller or the P2-CTLA Control Box must be connected.

[Focus operation]

Focus unit, focus mount, remote controller: Focus knob

The figure shows the turning directions of the focus knobs and the corresponding vertical movements of the focus mount.

 Turning the knob backward: Upward movement  Turning the knob forward: Downward movement

Foot switch: Left foot pedal

 Rear pedal: Upward movement  Front pedal: Downward movement

(See Chapter 4, “10.2 AZ-FSW Foot Switch.”)

Focusing on the Sample

Downward movement

Left rear pedal (Upward movement)

Upward movement

Coarse

DISPLAY

ESCAPE

ON/OFF

ALZ

ON/OFF

Z-SPEED

Fine

FL Cube

Ex Fine

−

ZOOM STEP

−+

ZOOM STEP

Z-RESET

0.5x

Upward movement

BRIGHTNESS

0.1x

LIMIT

ON/OFF

Downward movement

Left front pedal (Downward movement)

Notes on turning the focus knobs

 When using the focus knobs of the focus unit, focus mount or

the remote controller, do not turn (or twist) the right and left focus knobs in opposite directions at the same time. Doing so may cause malfunction.

 When the focus knobs of the focus unit or focus mount reach

their rotational limits for the unit’s vertical movement, do not forcibly turn it further. Doing so may cause malfunction.

Focus operation

1 x

SHR Plan apo WD:60

2 x

SHR Plan apo WD:20

Do not turn the knobs in the opposite direction!

Page 40

Chapter 3 Specific Operations

1 Check that the optical path of the tube is 100% to the binocular part.

2 Using coarse control, lower the zooming body to a position near the working distance.

[Working distances of objectives]

0.5x： 71 mm 1x： 60 mm

1.6x： 30 mm 2x： 20 mm

3 Using fine control, precisely focus on the sample.

 Torque of the focus knobs

For the P2-FU Focus Unit and P2-FMDN Focus Mount, the torque of the coarse control knobs can be changed. To do this, turn the torque adjustment ring on the base of the left coarse focus knob using a hex driver (nominal designation, 2: supplied with the focus unit). When the torque is too low, the vertical position of the zooming body may slide due to various loads (such as its own weight or the load of the counterbalance spring inside the focus unit). Be sure to adjust the torque to an appropriate level. Note that turning the fine control knob will cause the coarse control knob to also turn, changing the torque of the fine control knob.

Adjusting the torque of the focus knobs

Torque increase

WD:60

Apo

SHR

Torque adjustment ring

 Focusing stroke

 P2-FU Focus Unit

Focusing stroke: 100 mm

Stroke per rotation of the focus knobs Coarse control, 18.50 mm per rotation

Fine control, 3.27 mm per rotation

 P2-MFU Motorized Focus Unit

Focusing stroke: 100 mm

 P2-FMDN Focus Mount

Focusing stroke: 65 mm

Stroke per rotation of the focus knobs Coarse control, 18.50 mm per rotation

Fine control, 3.27 mm per rotation

Useful functions for focusing

When using the P2-MFU Motorized Focus Unit, the remote control enables the following remote operations: changing the focus speed, setting the limit position, setting retracting, and resetting Z. For details, see Chapter 4, “2.2 P2-MFU Motorized Focus Unit.”

* Also see Chapter 4, “2 Focus Units.”

Page 41

Chapter 3 Specific Operations

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

Besides changing the magnification with the zooming body, this microscope system enables the magnification to be changed by switching the objectives when used with the P2-RNI2 Intelligent Nosepiece and equipped with two objectives having different magnification ratios.

8.1

The magnification of the zooming body can be changed by zooming in and out.

[Image zooming]

SMZ18: Zooming knobs of the Zooming Body

SMZ25: Zoom buttons of the remote controller

Changing the Magnification

Image Zooming

The figure shows the turning directions of a knob and the corresponding changes in the magnification.

 Turning the knob backward: Magnification increase  Turning the knob forward: Magnification decrease

 Upper button: Magnification increase  Lower button: Magnification decrease

Pressing and holding the buttons causes the magnification to change continuously. Right foot pedal of the foot switch

 Rear-side pedal: Magnification increase  Front-side pedal: Magnification decrease

(See Chapter 4, “10.2 AZ-FSW Foot Switch.”)

■ Magnification and zoom ratio

SMZ18: 0.75 to 13.5x, 18:1 SMZ25: 0.63 to 15.75x, 25:1

Useful functions for image zooming

When using the SMZ25 Zooming Body, the magnification step size can be switched by pressing the zoom step switching button of the remote controller. The magnification can also be switched automatically so that the field of view before switching is maintained when the objective is switched (ALZ function). For details, see Chapter 4, “1.2 SMZ25 Zooming Body.”

Magnification increase

Magnification decrease

Image Zooming

Coarse

DISPLAY

Z-SPEED

ESCAPE

ON/OFF

ALZ

ON/OFF

Fine

Ex Fine

FL Cube

−

ZOOM STEP

−+

ZOOM STEP

0.5x

Z-RESET

Upper zoom button (magnification increase)

BRIGHTNESS

Lower zoom button (magnification decrease)

0.1x

LIMIT

ON/OFF

Zoom step indicator

Zoom step switching button

Right rear pedal (Magnification increase)

Right front pedal (Magnification decrease)

Page 42

Chapter 3 Specific Operations

Total magnification

The total magnification of the microscope is determined from the formula below:

Total magnification = zoom magnification x object’s magnification x eyepiece’s magnification  When using a vertical tube, replace the eyepiece’s magnification with the C mount adapter lens’ magnification to

calculate the total magnification.

* Also see Chapter 4, “1 Zooming Body.”

8.2

Switching Objectives (Using the P2-RNI2 Intelligent Nosepiece)

The P2-RNI2 Intelligent Nosepiece can be attached with two of the following objectives: P2-SHR Plan Apo 0.5X, 1X, 1.6X and 2X. The attached objectives can be switched by turning the nosepiece. Check that the objective falls into the click position when turning the nosepiece.

 Stereoscopic view and vertical view

An intelligent nosepiece has two click positions for each objective.

The click position switches between stereoscopic view and vertical view. Microscopy with vertical view is possible when needed, for example when taking a picture.

The click position of the objective can be checked by the position of the objective viewed from the front or the color of the hole on the objective status display area on the intelligent nosepiece.

* Also see Chapter 4, “3.1 P2-RNI2 Intelligent Nosepiece.”

Objective state

indication

Objective on the

tical path

Switching Objectives

Objective state

indication

SHR Plan apo WD:60

1 x

2 x

SHR Plan apo WD:20

White White

SHR Plan apo WD:60

1 x

SHR Plan apo WD:20

2 x

Black

Stereoscopic view Vertical view

Objective position state (stereoscopic view and vertical view)

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Chapter 3 Specific Operations

To view another part of a sample during microscopy, use the following procedure to shift the sample.

9.1

Turn the XY shift knob of the P-SXY64 XY Stage mounted instead of the stage plate to shift the sample on the glass plate and change the part to be observed.

 XY stroke of the stage with the X/Y shift knob and stroke

per rotation of the knob

X: 150 mm; 37.5 mm per rotation Y: 100 mm; 24.1 mm per rotation

Usage restriction

If the P-SXY64 XY Stage is mounted on the P-PS32 Plain Stand, the movement of the stroke in the Y direction is restricted.

* Also see Chapter 4, “5.5 P-SXY64 XY Stage.”

9.2

Remove the stage plate from the base and attach the C-SSL DIA Sliding Stage or C-TRS Tilting Stage in place so that the sample can be tilted or moved slightly.

Shifting a Sample

Shifting the Stage in the X and Y Directions (P-SXY64 XY Stage)

Shift using the P-SXY64 XY Stage

Using the Sliding Stage

Page 44

Chapter 3 Specific Operations

The SMZ18 and SMZ25 Zooming Bodies contain an aperture diaphragm.

Turn the aperture diaphragm dial to adjust the aperture.

The aperture can be adjusted in the range from fully open to 3 mm dia.

Opening and closing the aperture diaphragm causes the brightness and focus depth to change resulting in a change in resolution.

Open  Close

Brightness Bright Dark

Focus depth Shallow Deep

Resolution High Low

Using the Aperture Diaphragm

perture

dia

hragm dial

Adjusting the aperture diaphragm

The microscope system enables episcopic illumination when used with the devices listed below. All of the devices use LEDs as the light source and provide uniform illumination.

Illumination led from the light source by the fiber is emitted to a sample through an objective or directly so that an image resulting from the light reflected from the sample is observed. Episcopic illumination is used for observing samples which do not transmit light such as metal and mineral sections.

In addition, if the P2-CI Coaxial Epi Illuminator is attached, illumination along the optical axis for observation can also be emitted, which is effective for observing samples such as metallic mirrors, resins having smooth surfaces, and semiconductor

wafe

See Chapter 4, “7 Episcopic Illuminator” and select the appropriate device based on the objective of microscopy.

 P2-CI Coaxial Epi Illuminator  C-FDF Flexible Double Arm Fiber Illumination Unit  P2-FIR Ring Fiber Illumination Unit  P2-FIRL LED Ring Illumination Unit

For the operations of the light sources such as brightness adjustment, refer to the instruction manuals of the light sources.

Using Episcopic Illumination

Episcopic illumination with

the P2-CI Coaxial Epi Illuminator

Page 45

Chapter 3 Specific Operations

The microscope system enables diascopic illumination when used with the devices listed below. All of the devices use LEDs as the light source and provide uniform illumination.

The image to be observed results from diascopic illumination which passes through the sample and enters the objective. Diascopic illumination is used for observing transparent substances such as macromolecular compounds, fibers, and glass and tissue sections and biological specimens in petri dishes.

In addition, optional devices can be attached to enable dark-field diascopic illumination and diascopic simplified/polarized illumination. This allows for the observation of samples that are difficult to be observed under ordinary bright-field diascopic illumination such as transparent materials and crystals and tissues and minute organisms in culture solutions.

See Chapter 4 “8 Diascopic Illuminator” and select the appropriate device based on the objective of microscopy.

 P2-DBL LED Diascopic Illumination Base  P2-DBF Fiber Diascopic Illumination Base  P-DF LED Dark Field Unit  P2-POL Simple Polarizing Attachment

Using Diascopic Illumination

Diascopic illumination with the P2-DBL LED

Diascopic Illumination Base

Empty cube

When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical path.

OCC illumination

The P2-DBL LED Diascopic Illumination Base and P2-DBF Fiber Diascopic Illumination Base support OCC illumination (oblique illumination). OCC illumination, which offers high contrast, is appropriate for microscopy of phase objects.

Page 46

Chapter 3 Specific Operations

1 2 3 4

DISPLAYDISPLAY

BRIGHTNESSBRIGHTNESS

ESCAPEESCAPE

ALZALZ

−+

LIMITLIMIT

ZOOMZOOM STEPSTEP

Z-RESETZ-RESET

Z-SPEEDZ-SPEED ZOOM STEPZOOM STEP

CoarseCoarse

FL CubeFL Cube

ON/OFFON/OFF ON/OFFON/OFF

FineFine Ex FineEx Fine 1x1x 0.5x0.5x 0.1x0.1x

−

Epi-fluorescence microscopy used for observing fluorescence images requires an optical element such as a fluorescence filter, and a brighter light source including mercury lamp.

In this microscope system, state detection or motorized-type epi-fluorescence illumination is available. With a filter cube attached to the device, the filter cube is brought into the optical path by the rotation of the turret, resulting in the generation of epi-fluorescence illumination. The light source is a precentered high-intensity mercury lamp.

Illumination led from the light source to the device by the HG fiber passes through a fluorescence filter and emits excitation light to the sample so that fluorescent images can be observed.

See Chapter 4, “9 Epi-Fluorescence Attachment” and select the appropriate device based on the objective of microscopy.

 P2-EFLI Epi Fluorescence Attachment  P2-EFLM Motorized Epi Fluorescence Attachment

For details on adjusting the light sources such as brightness adjustment, refer to the instruction manuals of the light sources.

WARNING

The light source used with the epi fluorescence attachment (mercury lamp) requires special care during handling because of its characteristics. Make sure you are familiar with and observe all warnings and cautions described at the beginning of this instruction manual.

13.1

The turret of the epi fluorescence attachment is rotated to switch the four filter cubes attached to the turret.

Using Epi-Fluorescence Illumination

Switching Filter Cubes

Epi-fluorescence illumination with the epi

fluorescence attachment

[Turret rotation operation]

P2-EFLI Epi Fluorescence Attachment: Filter cube switching knob of the device P2-EFLM Motorized Epi Fluorescence Attachment: Filter cube switching buttons of the remote controller

Filter cube switching

BINO

PHOTO

buttons

Filter cube switching knob

Switching filter cubes

On the P2-EFLI Epi Fluorescence Attachment, turn the filter cube switching knob to assign to the indicator the turret address (FL1 to FL4) of the filter cube to be placed into the optical path.

On the remote control, press the filter cube switching buttons (FL1 to FL4) to specify the turret address of the filter cube to be placed into the optical path. The LED of the pressed button lights so that the turret address on the optical path can be identified. The motorized epi fluorescence attachment has a turret address view window on the right front so that the turret address on the optical path can be identified.

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Chapter 3 Specific Operations

13.2

The epi fluorescence attachment can be equipped with the following filter cubes:

 P2-EFL GFP-B, P2-EFL GFP-L, and P2-EFL RFP Filter Cube: Used for fluorescence observation with a given wavelength.  P2-EFLC Filter Cube: Used for fluorescence observation with an arbitrary wavelength when combined with arbitrary filters,

for example.

 P2-EFLBF Filter Cube (Bright Field): Used for bright-field episcopic observation.  Empty cubes (three cubes supplied with an epi-fluorescence attachment): Used when performing diascopic illumination

observation using the epi-fluorescence attachment.

13.3

 Using non-fluorescent slide glass and cover glass

When performing fluorescence observation of samples with a slide glass or cover glass, be sure to use non-fluorescent slide glass or cover glass to obtain a high-contrast image.

 Locating a target in the sample

For epi-fluorescence microscopy, Nikon recommend locating a target with bright-field diascopic illumination (OCC illumination) and then switching to epi-fluorescence rather than emitting strong excitation light to the sample from the beginning. When doing so, follow the operational tips below:

 Start with low magnification under bright-field diascopic illumination.  If the sample is not transparent, use the P2-EFLBF Filter Cube (Bright Field) to obtain the entire image by reflected light.  Gradually increase the magnification. When the target becomes difficult to locate, switch to epi-fluorescence and use

low excitation light.

Selecting a Filter Cube

Important Notes for Epi-Fluorescence Microscopy

 Protection from ultraviolet light (light shielding plate)

The light shielding plate is used to prevent the reflected ultraviolet light, which is originally emitted through the objective, from entering the observer’s eyes from the sample.

Using the light shielding plate

The microscope system enables remote control of several functions including focusing and changing magnification. The following remote control devices can be used when connected with the P2-CTLA Control Box:

 P2-RC Remote Controller  AZ-FSW Foot Switch  AZ-PCR Photo Release

For details, see Chapter 4 “10 Remote Control Device.”

Remote control from NIS-Elements is also possible by connecting the USB connector on the control box to a personal computer. For details, refer to the NIS-Elements instruction manual.

Remote Operation

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Chapter 3 Specific Operations

The microscope system can capture images of the sample being observed by attaching a camera such as a DS camera head to the vertical tube via a TV tube adapter or C-mount adapter.

15.1

Follow the procedure below to capture images. For the camera settings, see the instruction manual for the DS-L3 or DS-U3.

(1) Adjust the illumination of the microscope correctly and

the focus of the sample image.

(2) Adjust the mounting orientation of the DS camera head.

For details about attaching the camera head, see [5] Attach a Camera in Chapter 5, “3.3 Assembly of Other Devices (Optional).”

Since shifting a sample horizontally causes the image on the monitor to move horizontally, loosen the attachment guide fixing screw and adjust the orientation of the camera. After making the appropriate adjustments, tighten the screw.

Capturing Images

Procedure for Capturing Images

Using the DS-L3

Specify the following items beforehand.

 Folder to save  Name of the save file (or select Auto.)  File format and file size of the save file  Date and destination of the data

DS camera head

C-mount adapter

ttachment guide

fixing screw

LV-TV TV Tu be

dapter

Adjusting the camera orientation

(3) Select the camera scene mode suitable for the microscopy type.

(4) Adjust the camera’s white balance.

When using diascopic illumination, first capture an image of the top surface of the stage glass. When using episcopic illumination, first capture an image of any white subject. Then, press the WB button to execute white balance adjustment. (For fluorescent photomicrography, Nikon recommends adjusting the white balance under normal bright-field microscopy conditions before capturing images.)

(5) Adjust the position of the sample.

(6) Readjust the focus on the target.

(7) Adjust the image brightness using the camera exposure compensation function.

(8) Press the Pause button on the DS-L3 to check the image.

(9) Save the image if acceptable.

To save the image, press the capture button on the remote controller or DS-L3, or step on the pedal of the AZ-PCR Photo Release.

Page 49

Chapter 3 Specific Operations

15.2

 Confirming the photomicrographic range

The image on the monitor represents the photomicrographic range.

 Adjusting to eliminate extraneous light

Cover the eyepiece with a piece of cloth or similar item.

 Protecting fluorescent images from decoloration

The fluorescence of specimens may fade during exposure. To prevent this, do the following:

 Adjusting the excitation light

Excessively bright excitation light will accelerate the decoloration of the sample making it more difficult to obtain suitable fluorescent images. Use the ND filter of the light source to perform adjustment.

 Sample

Photomicrography of the color-fading portion requires a prolonged exposure time resulting in poor color reproduction and low-quality images. Move the sample so that the image of the portion that has not been exposed to excitation light can be captured. To obtain the best results, use the diascopic illumination method to select a specimen section for photomicrography, and then switch to the fluorescent method to capture the image.

Tips on Microscope Settings for Photomicroscopy

 Using the shutter

When not performing observation, close the shutter.

 Adjusting the brightness of the image on the monitor

When observing images on the monitor via a camera, the brightness can be changed also by making an adjustment on the camera side, for example, by changing the display mode, exposure mode and photometry mode, by compensating the exposure or by adjusting the image level.

For details, refer to the instruction manual for the DS-L3 or DS-U3.

Page 50

Chapter 3 Specific Operations

In stereo microscopes, the following information saved in the control box is read and used. The lower limit and the escape distance are previously set when the product is shipped. Other items are set to OFF or nothing is set. When using a motorized system or intelligent (status detection) system for the first time, set the items as required by following the specified procedures. For details, see the instruction manual supplied with the corresponding product.

Objective - NIS-Elements and setup tools, DS-L3

Eyepiece - Setup tools, DS-L3

Epi-fl filter cube - NIS-Elements and setup tools, DS-L3

Motorized focus unit lower limit 0 mm NIS-Elements, remote controller *

Motorized focus unit escape distance 20 mm NIS-Elements and setup tools

ALZ function OFF NIS-Elements and setup tools, remote controller *

Internally Saved Information

P2-CTLA Control Box

Items Default Tools

P2-CTLB Control Box

Items Default Tools

Objective - NIS-Elements and setup tools, DS-L3

Eyepiece - Setup tools, DS-L3

Epi-fl filter cube - NIS-Elements and setup tools, DS-L3

*: The setting can be changed but cannot be saved.

Page 51

Chapter

Functions and Operations of the Devices

This chapter describes the main functions and how to use the devices.

Page 52

Chapter 4 Functions and Operations of the Devices

Two types of zooming bodies are available: SMZ18 Zooming Body for manual zoom operations and SMZ25 Zooming Body for motorized zoom drive.

1.1

This model, which offers high NA, is designed for manual zoom operations.

For systems where a control box has been connected, the zoom information is sent to the control box via a motorized focus unit or relay box, and then transmitted to the DS-L3 DS Camera Control Unit and personal computer.

For the objective, the SHR-Plan Apo 0.5x, 1x, 1.6x, or 2x can be attached. When combined with the P2-RNI2 Intelligent Nosepiece, two objectives can be attached.

focus unit can be connected through the P2-RNI2 Intelligent

Nosepiece or the P2-FM Focus Mount Adapter.

The P2-FMDN Focus Mount can also be attached.

Zooming Body

SMZ18 Zooming Body

Usage restriction

Tubes, intermediate tubes and objectives of the previous SMZ series cannot be used.

Images can be zoomed in and out using the zooming knobs on the right and left sides of the zooming body.

Turning the knob backward: Magnification increase Turning the knob forward: Magnification decrease

Turning the knob causes the magnification to continuously change in the range from 0.75x to 13.5x (18:1 zoom ratio).

Magnification values are marked on the zooming knobs.

Click stop of the zooming knobs operates at the positions of

0.75x, 1x, 2x, 3x, 4x, 5x, 6x, 8x, 10x, 12x, and 13.5x. It is also possible to deactivate the click stop function.

 Activating and deactivating click stop of zoom operations

Insert the hex driver (nominal designation: 2) supplied with the focus unit into the hole on the rear side of the right zooming knob.

Turn the internal screw counterclockwise to activate click stop. Turn it clockwise to deactivate click stop.

Magnification decrease

Zoom operation

Magnification increase

0.75

Deactivating click stop

Activating click stop

Activating and deactivating click stop

Aperture diaphragm

The zooming body is equipped with an aperture diaphragm dial which enables the diaphragm of the right and left optical paths to be simultaneously adjusted in the range from fully open to 3 mm dia.

For details about using the aperture diaphragm, see Chapter 3, “10 Using the Aperture Diaphragm.”

zoom operations

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Chapter 4 Functions and Operations of the Devices

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

1.2

This model, which offers high NA, is designed for motorized zoom operations.

Zoom control is performed by the P2-CTLA Control Box. Control signals are sent to the SMZ25 via a motorized focus unit or relay box. The zoom information is sent to the control box via a motorized focus unit or relay box, and then transmitted to the DS-L3 DS Camera Control Unit and personal computer.

For the objective, the P2-SHR Plan Apo 0.5X, 1X, 1.6X or 2X can be attached. When combined with the P2-RNI2 Intelligent Nosepiece, two objectives can be attached.

focus unit can be connected through the P2-RNI2 Intelligent

Nosepiece or the P2-FM Focus Mount Adapter.

Usage restriction

Tubes, intermediate tubes and objectives of the previous SMZ series cannot be used

Images can be zoomed in and out using the zoom buttons on the remote controller.

Upper zoom button: Magnification increase Lower zoom button: Magnification decrease

Each time the button is pressed, the magnification changes in the range from 0.63x to 15.75x (25:1 zoom ratio) in steps. The step size depends on the setting of the zoom step switching button. Pressing and holding the button causes the magnification to change continuously.

 Magnification step size

Each time the zoom step switching button on the remote controller is pressed, the step size changes in the following order: 1x, 0.5x then 0.1x. When a step size is selected, the corresponding step LED indicator lights.

The right pedals of the foot switch can also be used to zoom in and out images as well as the zoom buttons on the remote controller.

Right rear pedal: Magnification increase Right front pedal: Magnification decrease

Each time the pedal is pressed, the magnification changes by one step. The step size depends on the setting of the zoom step switching button on the remote controller. Pushing and holding the pedal causes the magnification to change continuously.

SMZ25 Zooming Body

Upper zoom button (magnification increase)

Lower zoom button (magnification decrease)

Zoom step indicator

Zoom step switching button

Coarse

DISPLAY

Z-SPEED

ESCAPE

ON/OFF

ALZ

ON/OFF

Fine

Ex Fine

FL Cube

−

ZOOM STEP

−+

Z-RESET

ZOOM STEP

0.5x

BRIGHTNESS

0.1x

LIMIT

ON/OFF

Right rear pedal (magnification increase)

Right front pedal (magnification decrease)

Zoom operation

Aperture diaphragm

The zooming body is equipped with an aperture diaphragm dial which enables the diaphragm of the right and left optical paths to be simultaneously adjusted in the range from fully open to 3 mm dia.

For details about using the aperture diaphragm, see Chapter 3, “10 Using the Aperture Diaphragm.”

Page 54

Chapter 4 Functions and Operations of the Devices

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

x10x

Useful zoom operation functions (ALZ function)

When the objective is switched, the zoom magnification is automatically changed so that the field of view is maintained as it was.

This prevents the target image from moving or disappearing and makes it possible to observe the image while increasing or decreasing the zoom magnification.

To activate the ALZ function, press the ALZ button on the remote controller. The ALZ ON/OFF indicator lights. To deactivate the

LZ function, press the button again.

Example: Starting from an objective magnification of 0.5x, zoom magnification of 10x and eyepiece magnification of 10x

equaling a total magnification of 50x, switch the objective as follows;

Z-RESET

ZOOM STEP

0.5x

BRIGHTNESS

0.1x

LIMIT

ON/OFF

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

Ex Fine

ALZ button

ESCAPE

ON/OFF

ALZ

ON/OFF

−

ZOOM STEP

−+

ALZ ON/OFF

indicato

ALZ operations

MAG: 50x

ZOOM : 10x

OBJ : 0.5x EP10x

BRIGHTNESS

FL Cube

DISPLAY

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

Fine

Ex Fine

0.5x

0.1x

−

ZOOM

ESCAPE

LIMIT

STEP

ON/OFF

ALZ

−+

ON/OFF

0.5x

Deactivate the ALZ function. Activate the ALZ function.

Switching the objective from 0.5x to 2x

Switching the objective from 2x to 0.5x

MAG : 200x

ZOOM : 10x

OBJ : 2x EP10x

Total magnification changes from 50x to 200x. The field of view of the observed image changes.

MAG : 50x

ZOOM : 2.5x

OBJ : 2x EP10x

Zoom magnification automatically changes from 10x to 2.5x. Total magnification stays 50x and the field of view is maintained.

Deactivate the ALZ function. ALZ function stays active.

MAG : 12.5x

ZOOM : 2.5x

OBJ : 0.5x EP10x

Total magnification changes from 50x to 12.5x. The field of view of the observed image changes again.

Use example of the ALZ function

MAG : 50x

ZOOM : 10x

OBJ : 0.5x EP10x

Zoom magnification automatically changes from 2.5x to 10x. Total magnification stays 50x and the field of view is maintained.

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Chapter 4 Functions and Operations of the Devices

The P2-FU Focus Unit having a manual focus knob and the P2-MFU Motorized Focus Unit are available as focusing devices. In addition, the P2-FMDN Focus Mount is available for focus operations, and is attached to the support of the P-PS32 Plain Stand.

2.1

The P2-FU Focus Unit consists of a square-type support and a focus mount with the coaxial coarse/fine focusing system. The focus mount is attached with the SMZ18 or SMZ25 Zooming Body through the P2-RNI2 Intelligent Nosepiece or the P2-FM Focus Mount Adapter. The square support is attached with the P2-PB Plain Base, P2-DBL LED Diascopic Illumination Base or the P2-DBF Fiber Diascopic Illumination Base. Three types of stands can be configured.

Focus can be adjusted using the coarse/fine focus knobs on the right and left of the focus mount. The figure shows the turning directions of the focus knob and the corresponding vertical movements of the focus mount.

Turning the knob backward: Upward movement Turning the knob forward: Downward movement

 Focusing stroke: 100 mm  Stroke per rotation of the focus knob:

Coarse control, 18.50 mm/rotation Fine control, 3.27 mm/rotation

Notes on turning the focus knobs

 Do not turn (or twist) the right and left focus knobs in opposite

directions at the same time. Doing so may cause malfunction.

 When the focus knobs reach their rotational limits for the unit's

vertical movement, do not forcibly turn it further. Doing so may cause malfunction.

Focus Units

P2-FU Focus Unit

P2-FU Focus Unit (square support)

P2-FU Focus Unit (focus mount)

Upward movement

Focus knob (inner: coarse control/ outer: fine control)

Downward movement

P2-FU Focus Unit

1 x

SHR Plan apo WD:60

2 x

SHR Plan apo WD:20

Do not turn the knobs in the opposite direction!

Page 56

Chapter 4 Functions and Operations of the Devices

 Torque of the focus knobs

The torque of the coarse control knobs can be changed by turning the torque adjustment ring on the base of the left coarse focus knob using a hex driver (nominal designation, 2: supplied with the focus unit). When the torque is too low, the vertical position of the zooming body may slide due to various loads (such as its own weight or the load of the counterbalance spring inside the focus unit). Be sure to adjust the torque to an appropriate level. Note that turning the fine control knob will cause the coarse control knob to also turn, changing the torque of the fine control knob.

2.2

The P2-MFU Motorized Focus Unit consists of a square-type support and a focus mount with the motorized focusing system. The focus mount is attached with the SMZ18 or SMZ25 Zooming Body through the P2-RNI2 Intelligent Nosepiece or the P2-FM Focus Mount Adapter. The square support is attached with the P2-PB Plain Base, P2-DBL LED Diascopic Illumination Base or the P2-DBF Fiber Diascopic Illumination Base. Three types of motorized stands can be configured.

Focusing movement of the motorized focus unit is conducted using the focus knobs on the remote controller or the foot switch connected to the remote controller. The vertical position (Z coordinate value) is shown on the LCD on the remote controller.

Tool storage space

The focus unit has a storage space for storing three tools on its rear side. A hex driver and wrench supplied with the focus unit and the Intelligent nosepiece can be stored here.

P2-MFU Motorized Focus Unit

Z coordinate value

The accuracy of the Z coordinate value (vertical position) shown on the remote controller is not guaranteed. This value is used as a reference and includes a margin error.

Increase

WD:60

Apo

SHR

Torque adjustment ring

Adjusting the torque of the focus knobs

P2-MFU Motorized Focus Unit (square support)

P2-MFU Motorized Focus Unit (focus mount)

P2-MFU Motorized Focus Unit

 Initialization at power on

When the microscope system has been assembled and the power of the control box is turned on, the motorized focus unit is initialized as follows:

1 The power is turned on.

2 The focus mount moves upward to the upper limit.

3 The focus mount moves downward to the position before the power was turned on.

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Chapter 4 Functions and Operations of the Devices

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine1x1x

0.5x

0.1x

Focus knobs on the remote controller

Turning the knob backward: Upward movement of the focus mount Turning the knob forward: Downward movement of the focus mount

 Focusing stroke: 100 mm

Pedals of the foot switch

Left rear pedal: Upward movement of the focus mount Left front pedal: Downward movement of the focus mount

For details about the pedal operations, see “10.2 AZ-FSW Foot Switch” in this chapter.

Fine/coarse focus

switching button

Fine/coarse focus switching button

Focus knob

Upward movement

Downward movement

Focus speed indicato

Escape button

Escape ON/OFF indicato

Left rear pedal

(Upward movement)

Left front pedal

(Downward movement)

 Focus speed

The focus speed can be switched between coarse, fine, and extra fine control.

Use the fine/coarse focus switching button on the remote controller to switch the speed.

Each time the button is pressed, the focus speed is cyclically switched in the order of coarse, fine, and extra fine.

When the focus speed is changed, the corresponding indicator (Coarse/Fine/ExFine) lights.

 Coarse: 2 to 10 mm/second  Fine: 0.2 to 5 mm/second  Extra fine: 0.01 to 0.1 mm/second

Forced stop of motorized movement

The motorized focus unit is equipped with the STOP switch on the left side. Pressing the switch causes motorized movement to be forcibly stopped. To resume the motorized movement, perform the following steps:

1. Press the STOP switch again.

2. Turn off the power by pressing the power switch of the

P2-CTLB Control Box to the “” position.

3. Turn on the power by pressing the power switch to the “|” position. (After the power on, initialization at power on is automatically performed.)

FL Cube

DISPLAY

Z-SPEED

Coarse

Fine

Ex Fine

ESCAPE

ON/OFF

ALZ

ON/OFF

−

ZOOM STEP

−+

ZOOM STEP

Z-RESET

0.5x

0.1x

LIMIT

ON/OFF

Focus operation

BRIGHTNESS

STOP switch

Z-reset button

Focus knob

Limit button

Limit ON/OFF indicator

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Chapter 4 Functions and Operations of the Devices

Focus knob of the P2-MFU Motorized Focus Unit

The focus knob of the P2-MFU Motorized Focus Unit attached with a cover cannot be used. This knob is to be used by Nikon during maintenance.

Useful focus operation functions

The P2-MFU Motorized Focus Unit enables operations such as setting the limit position, setting retracting, and resetting Z using the remote controller.

 Limit position setting

When the limit button is pressed, the current position is set as the limit position so that the vertical movement part does not move down passing the limit point.

Pressing the limit button again cancels the limit position setting. When a limit position is set, the limit ON/OFF indicator below the button lights. Note that a limit position cannot be set in the range from the upper limit to 10 mm below that position. The default value of the limit position setting is 0 mm. The setting value can be changed using the NIS-Elements of remote controller.

 Escape setting

Pressing the Escape button causes the vertical movement part to move upward by the preset distance so that it stays in position. The vertical movement is not possible in this escape status. Pressing the Escape button again causes the vertical movement part to return to the previous position. Pressing and holding the Escape button causes the vertical movement part to exit escape status. When in escape status, the Escape ON/OFF indicator below the button lights.

The default value of the escape setting is 20 mm. The setting value can be changed using the setup tool or NIS-Elements.

 Z resetting

Pressing the Z-reset button displays 0 as the Z position (focus position) on the LCD of the remote controller. Pressing the button at another position sets that position as 0.

Also see “10.1 P2-RC Remote Controller” in this chapter.

Tool storage space

The focus unit has a storage space for storing three tools on its rear side. A hex driver and wrench supplied with the focus unit and the Intelligent nosepiece can be stored here.

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Chapter 4 Functions and Operations of the Devices

2.3

The P2-FMDN Focus Mount is used exclusively for the SMZ18.

djust the focus using the focus knob.

The focus mount can be attached to various stands having a support of 32 mm diameter.

P2-FMDN Focus Mount

Focus mount

Upward movement

Usage restriction

This focus mount cannot be combined and used with the P2-EFLM Motorized Epi Fluorescence Attachment.

The figure shows the turning directions of the focus knob and the corresponding vertical movements of the focus mount.

Turning the knob backward: Upward movement Turning the knob forward: Downward movement

 Focusing stroke: 65 mm  Stroke per rotation of the focus knob:

Coarse control, 18.50 mm/rotation Fine control, 3.27 mm/rotation

Notes on turning the focus knobs

 Do not turn (or twist) the right and left focus knobs in opposite

directions at the same time. Doing so may cause malfunction.

 When the focus knobs reach their rotational limits for the unit’s

vertical movement, do not forcibly turn it further. Doing so may cause malfunction.

Focus knob (inner: coarse control/ outer: fine control)

Downward movement

P2-FMDN Focus Mount

1 x

SHR Plan apo WD:60

 Torque of the focus knobs

The torque of the coarse control knobs can be changed by turning the torque adjustment ring on the base of the left coarse focus knob using the tool supplied with the stand. When the torque is too low, the vertical position of the zooming body may slide due to various loads (such as its own weight or the load of the counterbalance spring inside the focus unit). Be sure to adjust the torque to an appropriate level. Note that turning the fine control knob will cause the coarse control knob to also turn, changing the torque of the fine control knob.

Do not turn the knobs in the opposite direction!

0.75

Increase

WD:60

Apo

SHR

Torque adjustment ring

Adjusting the torque of the focus knobs

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Chapter 4 Functions and Operations of the Devices

3.1

The P2-RNI2 Intelligent Nosepiece can be attached with two objectives. The attached objectives can be switched by turning the nosepiece.

■ Stereoscopic view and vertical view

An intelligent nosepiece has two click positions for each objective.

The click position switches between stereoscopic view and vertical view. Microscopy with vertical view is possible when needed, for example when taking a picture.

The click position of the objective can be checked by the position of the objective viewed from the front or the color of the hole on the objective status display area on the intelligent nosepiece.

For systems where a control box has been connected, the objective attachment position addresses and vertical-view state are sent to the control box via a motorized focus unit or relay box, and then transmitted to the DS-L3 DS Camera Control Unit and personal computer.

Nosepiece

P2-RNI2 Intelligent Nosepiece

Objective state

indication

Nosepiece

Objective state indication

P2-RNI2 Intelligent Nosepiece

Objective state

indication

SHR Plan apo WD:60

1 x

2 x

SHR Plan apo WD:20

White White

SHR Plan apo WD:60

1 x

SHR Plan apo WD:20

2 x

Stereoscopic view Vertical view

Objective position state (stereoscopic view and vertical view)

P2-FM Focus Mount Adapter

The P2-RNI2 Intelligent Nosepiece also serves as the interface between the zooming body and focus unit. To connect the zooming body with the focus unit, the P2-FM Focus Mount Adapter must be connected.

P2-FM Focus Mount Adapter

Black

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Chapter 4 Functions and Operations of the Devices

The microscope system supports the following objectives.

Attach the objectives to the zooming body or intelligent nosepiece.

Model name P2-SHR Plan Apo 0.5X P2-SHR Plan Apo 1X P2-SHR Plan Apo 1.6X P2-SHR Plan Apo 2X

Magnification 0.5x 1x 1.6x 2x

Objectives

Numerical aperture (maximum)

Working distance

Parfocal distance:

Objective mount Exclusively-shaped

Others Protection ring provided Correction glass

0.078 0.156 0.25 0.312

71 mm 60 mm 30 mm 20 mm

150 mm 150 mm 150 mm 150 mm

bayonet

Exclusively-shaped bayonet

provided

Exclusively-shaped bayonet

With a correction ring

Exclusively-shaped bayonet

 The 2x objective is equipped with the correction ring for water 0 to 3 mm in depth. The correction ring compensates an

aberration caused by water in the dish. With this objective, select 2x magnification or higher. If magnification less than 2x is selected, shading will occur or part of the field of view will be missing.

 Attach the 1/4 lambda plate supplied with the coaxial epi illuminator to the position from which the protection ring supplied

with the 0.5x objective or the correction glass supplied with the 1x objective has been removed.

Usage restriction

 When the episcopic illuminator is used, use of the objectives and magnification are subject to some restrictions.

(See “7 Episcopic Illuminator” in this chapter.)

 When the diascopic illuminator is used, use of the objectives and magnification are subject to some restrictions.

(See “8 Diascopic Illuminator” in this chapter.)

Total magnification

The total magnification of the microscope is determined from the formula below:

Total magnification = zoom magnification x objective’s magnification x eyepiece’s magnification  When using a vertical tube, replace the eyepiece’s magnification with the C mount adapter lens’ magnification to

calculate the total magnification.

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Chapter 4 Functions and Operations of the Devices

Bases consist of various focus stands in combination with the focus units. The base models include a base without a built-in diascopic illumination system and a base with a built-in optical system for diascopic illumination.

A stand type is also available where a focus mount is connected to the support of the stand.

In all cases, a large stage plate (180 mm dia.) is used to enable more efficient work and easier observation of samples in containers such as petri dishes.

The stage plate can be replaced with a stage having an X/Y shift knob or a sliding stage. It is also possible to attach other devices such as a fiber illuminator or diascopic polarizing device to expand the base’s functions.

5.1

The plain base consists of a plain stand or a motorized plain stand in combination with the P2-FU Focus Unit or P2-MFU Motorized Focus Unit.

It is equipped with a stage plate having black and milky white planes. Select the plain based on the sample being observed.

The plain base has three indents for holding the base: front of the base’s top board and on the right and left rear.

5.2

See “8.1 P2-DBL LED Diascopic Illumination Base” in this chapter.

5.3

See “8.2 P2-DBF Fiber Diascopic Illumination Base” in this chapter.

Bases, Stand, and Stages

P2-PB Plain Base

Tap holes for attaching accessories

The base’s top board has tap holes for attaching various accessories. Tap holes prevent ingress of solution into the base.

(1) 2-M4: Positioned under the stage plate attachment

section of the base. Used for multiple purposes.

(2) 4-M6: Positioned under the stage plate attachment

section of the base, Used for attaching the P-SXY64 XY Stage.

(3) 2-M5: Positioned at the center on the top surface of

the base. Used for multiple purposes.

(4) 4-M4: Positioned at the center on the top surface of

the base. Used for multiple purposes.

(5) 4-M5: Positioned at the rear on the top surface of the

base. Used for attaching an accessory such as the C-FDF Flexible Double Arm Fiber Illumination Unit.

P2-DBL LED Diascopic Illumination Base

P2-DBF Fiber Diascopic Illumination Base

(2) to (5) are not penetrating to

P2-PB Plain Base

255

145

115

(2)

4-M6

Depth 8

Tap holes for attaching accessories

20 100 20

184

40 70 40

(5)

4-M5 Depth 7

(3)

2-M5 Depth 7

(4)

4-M4 Depth 6

(1)

2-M4

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Chapter 4 Functions and Operations of the Devices

5.4

This stand consists of a microscope system with the SMZ18 Zooming Body attached with the P2-FMDN Focus Mount.

It is equipped with a stage plate having black and milky white planes. Select the plane based on the sample being observed.

The stand support can be replaced with a long support. A special screwdriver is stored in the upper part of the support.

P-PS32 Plain Stand

Tap holes for attaching accessories

The base’s top board has tap holes for attaching various accessories. The tap holes are not penetrating to prevent ingress of solution into the base.

(1) 2-M4: Positioned under the stage plate attachment

part of the base. Used for multiple purposes.

(2) 4-M6: Positioned under the stage plate attachment

part of the base. Used for attaching the P-SXY64 XY Stage.

(3) 2-M4: Positioned at the center on the top surface of

the base. Used for multiple purposes.

(4) 4-M5: Positioned at the rear on the top surface of the

base. Used for attaching an accessory such as the C-FDF Flexible Double Arm Fiber Illumination Unit.

P-PS32 Plain Stand

20 100 20

150

175

115

(2)

4-M6

Depth 8

Tap holes for attaching accessories

(4)

4-M5 Depth 7

(3)

2-M4 Depth 6

(1)

2-M4

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Chapter 4 Functions and Operations of the Devices

5.5

This stage shifts the glass plate in the X and Y directions when the X/Y shift knobs are turned. Attach this stage instead of the base or the stage plate on the stand. The glass plate measures 215 mm x 154 mm x 5 mm and is detachable.

45-mm dia. filter can be slotted into the optical-path hole on the

lower board of the stage.

[Compatible bases and stand]

 P2-PB Plain Base  P2-DBL LED Diascopic Illumination Base  P2-DBF Fiber Diascopic Illumination Base  P-PS32 Plain Stand

(The movement of the stroke in the Y direction is restricted.)

 XY stroke of the stage with the X/Y shift knob and stroke per rotation of the knob

X: 150 mm; 37.5 mm per rotation Y: 100 mm; 24.1 mm per rotation

P-SXY64 XY Stage

Shift using the P-SXY64 XY Stage

 Load weight

5 kg or less

 Applicable range of diascopic illumination (bright field/ OCC)

150 x 100 mm travel range (Shading may occur in diascopic illumination or OCC illumination in the low zoom magnification range.)

5.6

This stage enables fine movement of a sample when its side is pushed.

Remove the stage plate of the base and attach it to the upper surface of the sliding stage. Then, mount the sliding stage to the base.

[Compatible bases]

 P2-PB Plain Base  P2-DBL LED Diascopic Illumination Base  P2-DBF Fiber Diascopic Illumination Base  P-PS32 Plain Stand

5.7

This stage enables tilt observation of samples. Attach the stage to the base or stand.

For details about usage, refer to the instruction manual for the C-TRS Tilting Stage.

[Compatible bases and stand]

 P2-PB Plain Base  P2-DBL LED Diascopic Illumination Base  P2-DBF Fiber Diascopic Illumination Base  P-PS32 Plain Stand

C-SSL DIA Sliding Stage

C-TRS Tilting Stage

C-SSL DIA Sliding Stage

C-TRS Tilting Stage

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Chapter 4 Functions and Operations of the Devices

This microscope system can be used with the tubes listed in the table below.

Attach the 10x, 15x, 20x, or 30x eyepiece to the binocular sleeve. Then, attach a camera such as a DS camera head to the vertical tube using an adapter.

The tube is equipped with the optical path switching lever. This allows the optical path to be switched during observation using the eyepiece and capture images using the DS camera. The distribution ratio of the light amount depends on the tube type.

Tubes

Optical path switching lever

P2-TERG 100/ P2-TERG 50 Trinocular Tilting Tube

P2-TL100 Trinocular Tube L

Optical path switching lever

Distribution of light with optical path switching (%)

Tube Lever position

P2-TERG 100 Trinocular Tilting Tube

P2-TERG 50 Trinocular Tilting Tube Pulled out 100 100 0

P2-TL100 Trinocular Tube L Pulled out 100 100 0

* The light amount of the left eye is always 100%. The light from the optical path of the right eye is diverged to the vertical tube. (An

image in the right eye’s optical path is captured.)

Pulled out 100 100 0

Pushed in 100 0 100

Pushed in 100 50 50

Pushed in 100 0 100

Binocular part

Left Right

Vertical tube*

6.1

This is an ergonomic tube that enables the angle of depression to

P2-TERG 100/ P2-TERG 50 Trinocular Tilting Tube

be adjusted.

The angle can be continuously changed by moving the binocular part as indicated by the arrow in the figure.

djust the angle to facilitate microscopy.

The eye level can be changed also by turning the binocular part 180°.

ngle of depression: 0 to 30° (continuously variable)

For the division of the light amount with the optical path switching lever, see the table above.

djusting the angle of depression

Notes on adjusting the angle of depression

 Do not apply excessive force to the binocular part during depression angle adjustment. It may cause unexpected failure or

malfunction.

 When moving the binocular part, be careful not to catch your fingers in the rotary section at the bottom of the binocular part.

6.2

P2-TL100 Trinocular Tube L

Angle of depression: 10°

For the distribution ratio of the light amount with the optical path switching lever, see the table above.

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Chapter 4 Functions and Operations of the Devices

The illuminators which can be used for this microscope system include a coaxial episcopic illuminator, which is attached between the zooming body and tube, a ring fiber illuminator, which is attached to the tip of the objective, and an illuminator that has a flexible fiber for adjusting the illumination direction.

7.1

ttach this illuminator to the position between the zooming body and the tube. The C-FLED2 LED Light Source for Fiber Illuminator is used as its light source. The light from the light source is led into the device by the two-branch fiber and episcopic illumination is generated along the optical axis. This illuminator is suitable for observing mirror-like samples.

1/4 λ plate is supplied with the illuminator to prevent flare at the imaging system due to light reflected in the optical system. Attach the plate to the tip of the 0.5x or 1x objective.

Before attaching the plate, remove the protection ring from the tip of the 0.5x objective, and remove the correction ring from the tip of the 1x objective.

The brightest illumination is generated when the while-circle indicator is at the front. Select a position that has the optimum brightness and contrast for observation.

Usage restriction

The type of objectives used for the coaxial epi illuminator and the recommended zoom magnification are shown below.

Episcopic Illuminator

P2-CI Coaxial Epi Illuminator

Coaxial epi

illuminato

1/4 λ plate

P2-CI Coaxial Epi Illuminator

Compatible objectives and recommended zoom magnification

Objective

SMZ18 Zooming Body SMZ25 Zooming Body

P2-SHR Plan Apo 0.5X 3.8x - 2.5x -

P2-SHR Plan Apo 1X 4x - 4x -

P2-SHR Plan Apo 1.6X Unusable Unusable

P2-SHR Plan Apo 2X Unusable Unusable

* Shading or vignetting may occur in the low zoom magnification range.

Zoom magnification

For details about using the light source, refer to the instruction manual for the light source.

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Chapter 4 Functions and Operations of the Devices

7.2

This illuminator is a fiber connected to the C-FLED2 LED Light Source for Fiber Illuminator for illuminating samples.

Two fibers are held by the C-FIDH Fiber Holder. The orientation of the holder’s arm can be adjusted so that the tips of the fibers face the samples for illumination. For details about using the fiber holder, see the “ C-FIDH Fiber Holder” on the next page.

Important notes on handling the fibers

Do not excessively bend the fibers or apply excess force to the fibers. Doing so may damage the fibers.

For details about using the light source, refer to the instruction manual for the light source.

 C-FIDH Fiber Holder

It holds the tip of the flexible double-arm fiber. Mount the base of the holder to the base (stand) of the microscope system. It holds the tips of the fibers with the two arms coming from the holder’s base. The arms can be freely positioned in any direction. The positions of the arms can be firmly locked by tightening the knobs at the joints of the arms. The arms are connected with a mechanism that enables the arms to be freely swung centered around a single axis (Z axis). This allows the arms to be pushed aside without having to unlock the arm joints. After that, if the arm position is returned by the rotation of the single axis, the arm will stop at the position that was locked first.

7.3

This illuminator is connected to the C-FLED2 LED Light Source for Fiber Illuminator. Attach the ring-shaped fiber illumination to the outer periphery of the objective in order to illuminate samples.

The edge face of the sample can be illuminated evenly using this ring-shaped fiber.

Usage restriction

The following two objectives can be used with the P2-FIR Ring Fiber Illumination Unit:

 P2-SHR Plan Apo 0.5X  P2-SHR Plan Apo 1X

Important notes on handling the fibers

Do not excessively bend the fibers or apply excess force to the fibers. Doing so may damage the fibers.

For details about using of the light source, refer to the instruction manual for the light source.

7.4

The ring illumination part is attached to the outer periphery in order to illuminate samples.

For details, refer to the instruction manual for the P2-FIRL LED Ring Illumination Unit.

C-FDF Flexible Double Arm Fiber Illumination Unit

C-FDF Flexible Double Arm Fiber illumination Unit

P2-FIR Ring Fiber Illumination Unit

P2-FIRL LED Ring Illumination Unit

Fibe

Apo

1 x

WD:60

Apo

2 x

WD:20

Fiber holde

(knob)

C-FIDH Fiber Holder

P2-FIR Ring Fiber Illumination Unit

To C-FLED2 LED Light Source for Fiber Illuminator

Fiber holder (single-axis rotary section)

To C-FLED2 LED Light Source for Fiber Illuminator

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Chapter 4 Functions and Operations of the Devices

The primary diascopic illuminator for this microscope system consists of an illumination base equipped with an illuminating optical system. A dark-field illuminator and polarizing illuminator are also available.

8.1

Diascopic Illuminator

P2-DBL LED Diascopic Illumination Base

Prism sheet IN/OUT lever

OCC adjustment knob

LED brightness control selection switch

INPUT

12VDC ,

3.5A

CTRL

ON OFF

LED brightness control terminal

C adapter input terminal

Left side

LED power source ON/OFF switch

LED light control dial

P2-DBL LED Diascopic Illumination Base

OCC adjustment knob

Right side

1.6-2x

0.5-1x

Prism sheet IN/OUT leve

OUT

This illumination base contains an optical system for diascopic illumination. Since the base supports OCC illumination, high-contrast observation of transparent samples is enabled.

The control box supplies the power for illumination control if the control box is connected. Otherwise, a 12 V AC adapter must be connected.

To turn on illumination, move the LED power ON/OFF switch on the front of the base to the “|” (ON) position. Use the LED brightness control dial to adjust the brightness. Turn the LED brightness control dial clockwise to increase the brightness as needed.

If the control box is connected, LED On/Off, brightness control, and light intensity monitoring are possible using the control box by assigning the illumination control right to the control box. Use the brightness control selection switch on the base to assign the control right to the base or to the control box.

 Brightness control selection switch Off: Assigns the control right to the base.  Brightness control selection switch On: Assigns the control right to the control box.

The stage plate of the base is large (180 mm dia.) and made of transparent glass to enable more efficient work and easier observation of samples in containers such as petri dishes. The base has three indents for holding the base: front of the base’s top board and on the right and left rear.

 OCC (oblique coherent contrast) illumination

The LED diascopic illumination base supports OCC illumination. OCC illumination is an oblique illumination method for observing the phase objects, where a sample is illuminated with a coherent illuminating light obliquely by shielding a part of the optical path using a shading plate. By this illumination method, a colorless transparent sample can be observed with relief and contrast.

To adjust the OCC contrast, slide the OCC adjustment knob at the right front of the base back and forth. Adjust until the optimum observation state is obtained in combination with the selected magnification and phase amount of the sample. For ordinary bright-field microscopy under diascopic illumination, set the OCC adjustment control to either the farthest or closest position. Sliding the control gradually forward or backward from that position will cause the OCC effect to increase. Sliding the control by 46 mm will result in completely shielded status. Since adjustment in two directions is thus possible, the direction of the OCC contrast can be reversed.

In addition, since the adjustment control has a scale, the control position can be reproduced.

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Chapter 4 Functions and Operations of the Devices

 Prism sheet

The LED diascopic illumination base contains a prism sheet to reduce shading. When using the 1.6x or 2x objective, push in the prism sheet IN/OUT lever. When using the 0.5x or 1x objective, pull out the lever.

Compatible objectives and recommended zoom magnification

Objective

P2-SHR Plan Apo 0.5X 1.3x - Contrast in the field of view

P2-SHR Plan Apo 1X Entire area 5.0x-

P2-SHR Plan Apo 1.6X Entire area 4.0x -

P2-SHR Plan Apo 2X 2.0x - 3.0x -

* Shading may occur in the low zoom magnification range.

Increasing contrast of the OCC observation image

For observations of the OCC observation image with higher contrast, remove the filter dropping ring (attached to the base optical path immediately under the grass plate). The contrast can be increased by removing the filter dropping ring that contains a diffuser plate to control excess contrast for OCC.

45-mm dia. filter

A 45-mm dia. filter (no thicker than 5 mm) can be dropped onto the filter dropping ring (attached to the base optical path immediately under the grass plate).

Tap holes for attaching accessories

The base’s top board has tap holes for attaching various accessories. Tap holes prevent ingress of solution into the base.

(2) to (5) are not penetrating to

Bright-field diascopic illumination OCC illumination

Zoom magnification

is not uniform.

20 100 20

(1) 2-M4: Positioned under the stage plate attachment

section of the base. Used for multiple purposes.

(2) 4-M6: Positioned under the stage plate attachment

section of the base. Used for attaching the P-DF LED Dark Field Unit.

255

145

115

184

40 70 40

(3) 2-M5: Positioned at the center on the top surface of

the base. Used for multiple purposes.

(4) 4-M4: Positioned at the center on the top surface of

the base. Used for multiple purposes.

(5) 4-M5: Positioned at the rear on the top surface of the

base. Used for attaching an accessory such as the C-FDF Flexible Double Arm Fiber Illumination Unit.

(2)

4-M6

Depth 8

Tap holes for attaching accessories

(1)

2-M4

(5)

4-M5 Depth 7

(3)

2-M5 Depth 7

(4)

4-M4 Depth 6

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Chapter 4 Functions and Operations of the Devices

8.2

This illumination base contains an optical system for episcopic illumination. Since the base supports OCC illumination, high-contrast observation of transparent samples is enabled. The C-FLED2 LED Light Source for Fiber Illuminator is used as the light source. The fiber should be connected to the rear side of the base. For details about using the light source, refer to the instruction manual for the light source.

 OCC (oblique coherent contrast) illumination

The fiber diascopic illumination base supports OCC illumination. OCC illumination is an oblique illumination method for observing the phase objects, where a sample is illuminated with a coherent illuminating light obliquely by shielding a part of the optical path using a shading plate. By this illumination method, a colorless, transparent sample can be observed with relief and contrast.

To adjust the OCC contrast, turn the OCC adjustment knob. Adjust until the optimum observation state is obtained in combination with the selected magnification and phase amount of the sample. In addition, since the OCC adjustment knob has a scale, the position of the knob can be reproduced.

P2-DBF Fiber Diascopic Illumination Base

Condenser lens switching lever

Filter IN/OUT lever

OCC adjustment knob

P2-DBF Fiber Diascopic Illumination Base

Fiber

Fiber fixing screw

 Condenser lens

The base contains condenser lenses for low/high-magnification microscopy. Switch the condenser lens based on the objective being used to ensure uniform diascopic illumination. A wide range of observation magnifications are supported. To switch the condenser lens, slide the condenser lens switching lever to the right or left.

Slide the lens switching lever to the right: For low magnification; objective lens 0.5x to 1x Slide the lens switching lever to the left: For high magnification; objective lens 1.6x to 2x

When the condenser lens is switched, the built-in prism sheet is automatically switched and the shading adjusted.

Compatible objectives and recommended zoom magnification

Zoom magnification

Objective

P2-SHR Plan Apo 0.5X plus condenser lens for low magnification 1.5x - 4.5x -

P2-SHR Plan Apo 01X plus condenser lens for low magnification Entire area 4.0x -

P2-SHR Plan Apo 1.6X plus condenser lens for high magnification Entire area 4.0x -

P2-SHR Plan Apo 2X plus condenser lens for high magnification 2.0x - 6.0x-

* Shading may occur in the low zoom magnification range.

Bright-field diascopic

illumination

OCC illumination

 Filter

The base has three different filters: NCB11, ND4, and ND16. The individual filters can be inserted into and removed from the optical path separately. To insert a filter into the optical path, push in the filter IN/OUT lever.

45-mm dia. filter

A 45-mm dia. filter (no thicker than 2 mm) can be dropped onto the filter dropping ring (attached to the base optical path immediately under the grass plate).

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Chapter 4 Functions and Operations of the Devices

Tap holes for attaching accessories

The base’s top board has tap holes for attaching various accessories. Tap holes prevent ingress of solution into the base.

(2) to (5) are not penetrating to

(1) 2-M4: Positioned under the stage plate attachment

section of the base. Used for multiple purposes.

(2) 4-M6: Positioned under the stage plate attachment

section of the base. Used for attaching the P-SXY64 XY Stage.

(3) 2-M5: Positioned at the center on the top surface of

the base. Used for multiple purposes.

20 100 20

(5)

4-M5 Depth 7

(3)

2-M5 Depth 7

(4)

4-M4 Depth 6

255

184

40 70 40

145

115

(4) 4-M4: Positioned at the center on the top surface of

the base. Used for multiple purposes.

(5) 4-M5: Positioned at the rear on the top surface of the

base. Used for attaching an accessory such as the C-FDF Flexible Double Arm Fiber Illumination Unit.

(2)

4-M6

Depth 8

Tap holes for attaching accessories

(1)

2-M4

8.3

P-DF LED Dark Field Unit

This dark-field illumination unit has LEDs that are tilted and positioned like a ring. The unit is attached to the base of the microscope. For details, refer to the instruction manual for the P-DF LED Dark Field Unit.

8.4

This is a simple polarizing device that consists of a polarizer unit and analyzer unit.

First remove the stage glass from the diascopic illumination base.

ttach the base plate of the polarizer unit, orient the polarizer as required, and then set the 90 mm dia-stage glass supplied with the device. The stage glass can be rotated. Attach the analyzer to the tip of the objective and tighten the clamp screw to stabilize it. To turn the analyzer, turn the knurled ring at the tip of the objective. The polarizer and analyzer have indicators which show the orientation of the polarizing plate.

P2-POL Simple Polarizing Attachment

Directly placing the polarizer

When the LED diascopic illumination base is in use, the polarizer can be removed from the polarizer attachment and can be individually attached to the diascopic illumination base. Fit it under the stage plate of the diascopic illumination base. The stage plate can be used as is.

nalyzer unit

Polarizer unit

nalyzer

Clamp screw

nalyzer

rotating dial

Polarizer

Base plate

P2-POL Simple Polarizing Attachment

Usage restriction

Objectives P2-SHR Plan Apo 1.6x and 2x are not compatible.

The objective P2-SHR Plan Apo 1x covers the entire zoom area. The objective P2-SHR Plan Apo 0.5x covers a 1x or larger zoom area.

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Chapter 4 Functions and Operations of the Devices

The microscope system can be used with the status detection model P2-EFLI Epi Fluorescence Attachment and Motorized P2-EFLM Epi Fluorescence Attachment.

9.1

This illuminator is attached between the zooming body and tube.

HG precentered fiber illuminator is used as the light source. The light coming from the light source is led into the device by the HG fiber and then passed through the filter cube attached to the internal turret for epi-fl illumination.

The turret can be attached with up to four filter cubes; the addresses of the attachment positions are FL1 to FL4. For the attachable filter cube types, those for fluorescence, bright-field, and empty are available. Turn the filter cube switching knob to assign to the indicator the turret address (FL1 to FL4) of the filter cube to be placed into the optical path.

For systems where a control box has been connected, the information about the turret address on the optical path is sent to the control box via a motorized focus unit or relay box, and then transmitted to the DS-L3 DS Camera Control Unit and personal computer.

CAUTION

 Do not use the epi-fluorescence attachment while the filter cube replacement cover is open. Harmful light including ultraviolet

light may leak and affect fluorescence observation.

 Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become

hot, resulting in burns. Do not touch the front face of the empty cube. Replace the empty cube several minutes after finishing the diascopic observation.

Epi-Fluorescence Attachment

P2-EFLI Epi Fluorescence Attachment

Index mark

Turret address

indication

BINO

PHOTO

Filter cube indication label (label attachment position)

Filter cube replacement hole cove

Filter cube switching knob

P2-EFLI Epi Fluorescence Attachment

Be sure to attach four cubes to the turret.

Attach an empty cube to the position to which no filter cube is attached.

 Filter cube

 P2-EFL GFP-B, P2-EFL GFP-L, and P2-EFL RFP Filter Cubes: Used for fluorescence observation with a given

wavelength.

 P2-EFLC Filter Cube: Used for fluorescence observation with an arbitrary wavelength when combined with arbitrary

filters, for example.

 P2-EFLBF Filter Cube (Bright Field): Used for bright-field episcopic observation.  Empty cube (Three cubes are supplied with an epi-fluorescence attachment): Used when performing diascopic

illumination observation using the epi-fluorescence attachment.

Light shielding plate

The device is equipped with a light shielding plate for preventing the reflection of ultraviolet light. Use the light shielding plate when performing fluorescence microscopy. When in use, the light shielding plate can be pushed aside to positions up to 45° horizontally to the rear. When not in use, the plate can be pushed aside to positions up to 170°.

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Chapter 4 Functions and Operations of the Devices

Bright-field episcopic observation

This is useful for observing the entire image of the opaque specimen during fluorescence observation. Although the P2-EFLBF Filter Cube contains the UV blocking filter, adjust the brightness of the illuminator so that illumination is not too bright. When observing light reflected from the specimen with a mirror-like surface, the brightness on the right path differs from that on the left optical path. Also, duplicated images may be produced by internal half mirror.

OCC light shielding plate

When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in the diascopic illuminator.

For details about using the light source, refer to the instruction manual for the light source.

9.2

The motorized epi-fluorescence illuminator is attached between the zooming body and tube. A HG precentered fiber illuminator is used as the light source. The light coming from the light source is led into the device by the HG fiber and then passed through the

P2-EFLM Motorized Epi Fluorescence Attachment

Filter cube indication label (label attachment position)

filter cube attached to the internal turret for epi-fl illumination.

The turret can be attached with up to four filter cubes; the addresses of the attachment positions are FL1 to FL4. For the attachable filter cube types, those for fluorescence, bright-field, and empty are available. On the remote control, press the filter cube switching buttons (FL1 to FL4) to specify the turret address of the filter cube to be placed into the optical path. The LED of the pressed button lights so that the turret address on the optical path

P2-EFLM Motorized Epi Fluorescence Attachment

Filter cube replacement hole cover

Turret address check window

can be identified. The attachment has a turret address check window on the right front so that the turret address on the optical path can be identified.

CAUTION

 Do not use the epi-fluorescence attachment while the filter cube replacement cover is open. Harmful light including ultraviolet

light may leak and affect fluorescence observation.

 Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become

hot, resulting in burns. Do not touch the front face of the empty cube. Replace the empty cube several minutes after finishing the diascopic observation.

Be sure to attach four cubes to the turret.

Attach an empty cube to the position to which no filter cube is attached.

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Chapter 4 Functions and Operations of the Devices

 Filter cube

 P2-EFL GFP-B, P2-EFL GFP-L, and P2-EFL RFP Filter Cubes: Used for fluorescence observation with a given

wavelength.

 P2-EFLC Filter Cube: Used for fluorescence observation with an arbitrary wavelength when combined with arbitrary

filters, for example.

 P2-EFLBF Filter Cube (Bright Field): Used for bright-field episcopic observation.  Empty cube (Three cubes are supplied with an epi-fluorescence attachment): Used when performing diascopic

illumination observation using the epi-fluorescence attachment.

For details about using the light source, refer to the instruction manual for the light source.

Light shielding plate

Bright-field episcopic observation

OCC light shielding plate

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Chapter 4 Functions and Operations of the Devices

1 2 3 4

DISPLAY

BRIGHTNESS

ESCAPE

ALZ

LIMIT

ZOOM STEP

Z-RESET

Z-SPEED

ZOOM STEP

Coarse

FL Cube

ON/OFF

Fine

Ex Fine

0.5x

0.1x

A remote controller, foot switch, and photo release are available as remote control devices of the microscope system.

10.1

The P2-RC Remote Controller is used mainly for focus and zoom operations.

The remote controller is attached to the REMOTE connector of the P2-CTLA Control Box.

(6) Filter cube switching

(4) Fine/coarse focus

Remote Control Device

P2-RC Remote Controller

(1) LCD

button

switching button

(3) Focus knob

FL Cube

BRIGHTNESS

(9) Capture button

(2) Backlight control dial

(4) Fine/coarse focus

switchin

button

(8) Z-reset button

(3) Focus knob

(7) LCD display switching

(5) Focus speed indicato

(13) Escape button

(14) Escape ON/OFF

(17) ALZ button

(18) ALZ ON/OFF

(19) Diascopic LED

brightness control button

button

indicato

Coarse

DISPLAY

Z-SPEED

ESCAPE

ON/OFF

ALZ

ON/OFF

Fine

Ex Fine

−+

−

ZOOM STEP

1x1x0.5x

Z-RESET

LIMIT

ON/OFF

(10) Zoom button

(12) Zoom step indicator

0.1x

(11) Zoom step switching

button

(15) Limit button

(16) Limit ON/OFF indicator

(20) Diascopic LED ON/OFF

button

(21) Diascopic LED ON/OFF

indicato

(2) Backlight control dial

(4) Fine/coarse focus

switching button (on both sides)

Front Right Side

P2-RC Remote Controller

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Chapter 4 Functions and Operations of the Devices

 Functions and indications of the operation buttons

No. Button/Indication Function/Description

(1) LCD This 4 x 16 LCD displays magnification, filter cube names, and other

information. (See “ LCD indications.”)

(2) Backlight control dial Adjusts the backlight of the LCD. When turned fully clockwise, the LCD’s

backlight and the LED’s are turned off.

(3) Focus knob Vertically moves the focus mount of the P2-MFU Motorized Focus Unit. Turn

backward to move upward. Turn forward to move downward.

(4) Fine/coarse focus switching button Switches the focus speed. Each time the button is pressed, the speed is

cyclically switched in order of coarse, fine, and extra fine.

(5) Focus speed indicator When the focus speed is changed, the corresponding indicator

(Coarse/Fine/ExFine) lights.

(6) Filter cube switching button Turns the turret of the motorized epi fluorescence attachment. The filter cube

of the address of the pressed button is brought into the optical path and the LED of the corresponding button (address on the optical path) lights.

(7) LCD display switching button Switches the display on the LCD. Two pages can be switched for display. (See

“ LCD indications.”)

(8) Z-reset button Resets the indication of the Z position (focus position) on the LCD to 0.

Pressing at another position sets that position as 0.

(9) Capture button Captures images using a DS camera.

(10) Zoom button Changes the magnification of the zooming body. Pressing the upper button

causes the magnification to increase. Pressing the lower button causes the magnification to decrease. Pressing and holding the button causes the magnification to change continuously.

(11) Zoom step switching button Switches the zoom step size in order of 1x, 0.5x, and 0.1x.

(12) Zoom step indicator When the zoom step size is changed, the corresponding LED (1x, 0.5x, and

0.1x) lights.

(13) Escape button Moves the vertical movement part upward by the preset distance so that it

stays in position. The vertical movement is not possible in this escape status. Pressing the Escape button again causes the vertical movement part to return to the previous position. Pressing and holding the Escape button causes the vertical movement part to exit escape.

The travel distance can be newly set or changed using the setup tool or NIS-Elements.

(14) Escape ON/OFF indicator The LED lights when in escape status.

(15) Limit button When the limit button is pressed, the current position is set as the limit position

so that the vertical movement part does not move down passing the limit point. Pressing the limit button again cancels the limit position setting.

(16) Limit ON/OFF indicator The LED lights when the limit position has been set.

(17) ALZ button Activates the ALZ function. To deactivate the ALZ function, press the button

again. For the ALZ function, see “ Useful zoom operation functions (ALZ function)” in “1.2 SMZ25 Zooming Body” in this chapter.

(18) ALZ ON/OFF indicator The LED lights when the ALZ function is activated.

(19) Diascopic LED brightness control

button

(20) Diascopic LED ON/OFF button Turns diascopic LED illumination on and off.

(21) Diascopic LED ON/OFF indicator The LED lights when diascopic LED illumination is turned on.

Changes the brightness of the diascopic LED illumination. Press [+] to increase the brightness and [-] to decrease the brightness.

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Chapter 4 Functions and Operations of the Devices

 LCD indications

1st page

1: Total magnification (zoom magnification x objective

magnification x eyepiece magnification) (Example) [MAG: 236.25x]

2: Zoom magnification

(Example) [ZOOM: 15.75x]

3: Objective magnification

(Example) [OBJ: 0.75x]

4: Eyepiece magnification

(Example) [EP10x]

5: Diascopic LED light intensity

(Example) [LEDDIA      _______ ]

6: Field of view

] Stereoscopic view or [ ○] Vertical view

[

○○

2nd page

1: Actual field of view

(Example) [FOV: 24.44 mm]

2: P-PS32 Plain Stand Z-coordinate

(Example) [Z: 100.000 mm]

3-6: Filter cube name (all)

(Example) [1: GFP-B]

7: Field of view

[

] Stereoscopic view or [ ○] Vertical view

○○

10.2

AZ-FSW Foot Switch

The AZ-FSW Foot Switch is a controller that enables a pedal to be used for focus and zoom operations.

The foot switch is connected to the FOOT connector of the P2-CTLA Control Box. To use the foot switch, the P2-RC Remote Controller must be connected to the P2-CTLA Control Box.

(1) Left rear of the foot pedal

(Motorized focus unit Z-coordinate UP switch)

(2) Right rear of the foot pedal

(Motorized magnification UP switch)

1 6

3 4

1 7

3 4

5 6

(1) Left front of the foot

pedal (Motorized focus unit Z-coordinate DOWN switch)

(2) Right front of the

(3) Foot rest

foot pedal (Motorized magnification DOWN switch)

AZ-FSW Foot Switch

Connector attachment section

(viewed from arrow A)

PHOTO CTRL

(4) CTRL connector

(5) PHOTO connector

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Chapter 4 Functions and Operations of the Devices

No. Operation Section Function

(1) Left foot pedal Left rear foot pedal (motorized focus unit Z-coordinate DOWN switch)

 Drives the motorized focus unit downward.  When pressed, lowers the unit by 1.6 μm. Press and hold to move the unit continuously.  The speed changes based on the magnification and nosepiece address. (See the table

below.)

 If a nosepiece address has not been set, the unit moves at the maximum speed.

Left front of the foot pedal (motorized focus unit Z-coordinate UP switch)

 Drives the motorized focus unit upward.  When pressed, raises the unit by 1.6 μm. Press and hold to move the unit continuously.  The speed changes based on the magnification and nosepiece address. (See the table

below.)

 If a nosepiece address has not been set, the unit moves at the maximum speed.

Coarse

Magnification

Nosepiece address

SMZ25 0.63 to 5.0 5.1 to 10.0 10.1 to 15.75

SMZ18 0.75 to 4.0 4.1 to 8.0 8.1 to 13.5

10 (mm/second)

Fine

Magnification

Nosepiece address

Magnification

Nosepiece address

(2) Right foot pedal Right rear of the foot pedal (motorized magnification UP switch)

 Increases the magnification by one step. The step size depends on the zoom step size

setting.

 Press and hold to increase the magnification continuously.  The zoom step size depends on the setting of the zoom step switching button on the remote

controller.

Right front of the foot pedal (motorized magnification DOWN switch)

 Decreases the magnification by one step. The step size depends on the zoom step size

setting.

 Press and hold to decrease the magnification continuously.  The zoom step size depends on the setting of the zoom step switching button on the remote

controller.

SMZ25 0.63 to 5.0 5.1 to 10.0 10.1 to 15.75

SMZ18 0.75 to 4.0 4.1 to 8.0 8.1 to 13.5

1 5 (mm/second) 4 (mm/second) 2 (mm/second)

2 2 (mm/second) 1 (mm/second) 0.5 (mm/second)

Extra fine

SMZ25 0.63 to 5.0 5.1 to 10.0 10.1 to 15.75

SMZ18 0.75 to 4.0 4.1 to 8.0 8.1 to 13.5

1 0.5 (mm/second) 0.4 (mm/second) 0.2 (mm/second)

2 0.2 (mm/second) 0.1 (mm/second) 0.0.5 (mm/second)

(3) Foot rest Used as a foot rest.

(4) CTRL connector Attach to the FOOT connector of the P2-CTLA Control Box using the supplied cable.

(5) PHOTO connector Connect the AZ-PCR Photo Release. (See “10.3 AZ-PCR Photo Release” in this chapter.)

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Chapter 4 Functions and Operations of the Devices

10.3

The AZ-PCR Photo Release is a foot switch for capturing images using the camera connected to the microscope.

The photo release is connected to the PHOTO connector of the

Z-FSW Foot Switch.

For operations using a DS camera control unit such as the DS-L3 after a DS camera head has been attached to the microscope, connect the DS camera control unit to the P2-CTLA Control Box (USB connection). Next, connect the photo release to the

Z-FSW Foot Switch connected to the control box. The camera can then capture images simply by stepping on the pedal of the photo release.

AZ-PCR Photo Release

Connection cable

Foot switch

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Chapter 4 Functions and Operations of the Devices

11.1

When electrically connected to the following devices, the P2-CTLB Control Box can detect the device states and handle communications:

No. Device Operation

Control/Communications Device and Relay Device

P2-CTLB Control Box

Power LED

Power switch

P2-CTLB Control Box

(1) SMZ18 Zooming Body Detects the magnification.

(2) P2-DBL LED Diascopic Illumination Base Turns On/Off the LED illumination, controls the illumination, and

monitors the light intensity.

(3) P2-RLY Relay Box Relays communications.

(4) P2-EFLI Epi Fluorescence Attachment Detects the turret address on the optical path.

(5) P2-RNI2 Intelligent Nosepiece Detects the nosepiece address on the optical path.

(6) C-HGFIE HG Precentered Fiber Illuminator Switches the ND filter and opens/closes the shutter.

(7) C-HGFIE HG Precentered Fiber Illuminator Communications

(8-1) DS-L3 DS Camera Control Unit plus DS

camera

(8-2) Personal computer plus DS-U3 DS Camera

Control Unit plus DS camera

(9) AC adapter 24 V power input

Communications

 The power supply for control provides power for the board in the control box and devices (1) to (5).  Devices (1), (4), and (5) are connected through device (3).  One type-B USB connector is equipped so that device (8-1) or (8-2) can be connected. Note that both devices cannot be

connected at the same time.

 To operate using a personal computer after connecting device (8-1), connect the USB cable from the personal computer

to device (8-1).

 Communications with device (7) are handled through a RS232C connection. Communications with device (8-1) or (8-2)

are handled through a USB connection.

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Chapter 4 Functions and Operations of the Devices

 Connector names and connection destinations

The figure below shows the connectors on the rear of the control box and the connection destinations:

USB (Connect to the USB port of a personal computer or the DS-L3.)

LEDDIA (Connect to the LED light control terminal of the P2-DBL LED Diascopic Illumination Base.)

RELAY (Connect to the P2-RLY Relay Box

HGFIE (Connect to the RS232C port of the C-HGFIE HG Precentered Fiber Illuminator.)

Rear of the P2-CTLB

For the power cable connected to the AC adapter, use the one specified in Chapter 8, “2 Performance Properties.”

or P2-RLYC Relay Cable.)

DC24V IN (Connect to the 24 V AC adapter.)

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Chapter 4 Functions and Operations of the Devices

11.2

When electrically connected to the following devices, the P2-CTLA Control Box can perform motorized control of the devices, detect the device states, and handle communications:

No. Device Operation

(1) SMZ25 Zooming body Motorized control of magnification

(2) SMZ18 Zooming body Detects the magnification.

(3) P2-MFU Motorized Focus Unit Motorized control of focus

P2-CTLA Control Box

Power LED

Power switch

P2-CTLA Control Box

(4) P2-DBL LED Diascopic Illumination Base Turns On/Off the LED illumination, controls the illumination, and

monitors the light intensity.

(5) P2-RLY Relay Box Relays communications.

(6) P2-EFLM Motorized Epi Fluorescence

Attachment

(7) P2-EFLI Epi Fluorescence Attachment Detects the turret address on the optical path.

(8) P2-RNI2 Intelligent Nosepiece Detects the nosepiece address on the optical path.

(9) P2-RC Remote Controller Communications

(10) AZ-FSW Foot Switch Detects the switch states, moves the P2-MFU Motorized Focus Unit

(11) AZ-PCR Photo Release Detects the switch states and captures images.

(12) C-HGFIE HG Precentered Fiber Illuminator Switches the ND filter and opens/closes the shutter.

(13) C-HGFIE HG Precentered Fiber Illuminator Communications

(14-1) DS-L3 DS Camera Control Unit plus DS camera Communications

(14-2) Personal computer plus DS-U3 DS Camera

Control Unit plus DS camera

(15) AC adapter 24 V power input

Motorized control of turret rotation

vertically, and adjust the magnification of the SMZ25 Zooming Body.

Communications

 The power supply for control provides power for the board in the control box and devices (1) to (11).  Devices (1), (2), (6), (7), and (8) are connected through device (3) or (5).  One type-B USB connector is equipped so that device (14-1) or (14-2) can be connected. Note that both devices cannot

be connected at the same time.

 To operate using a personal computer after connecting device (14-1), connect the USB cable from the personal computer

to device (14-1).

 Communications with device (9) are handled through serial communications, communications with device (13) are

handled through a RS232C connection, and communications with device (14-1) or (14-2) are handled through a USB connection.

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Chapter 4 Functions and Operations of the Devices

ZOOM

 Connector names and connection destinations

The figure below shows the connectors on the rear of the control box and the connection destinations:

USB

FOOT (Connect to the CTRL connector of the AZ-FSW Foot Switch using the cable supplied with the foot switch.)

REMOTE (Connect to the P2-RC Remote Controller.)

HGFIE (Connect to the RS232C port of the C-HGFIE HG Precentered Fiber Illuminator.)

Rear of the P2-CTLA

Use the power cable specified in Chapter 8, “2 Performance Properties.”

11.3

When electrically connected to the following devises, the P2-RLY

P2-RLY Relay Box

View

Relay Box can relay signals for motorized control of the devices and detect the device states:

(1) P2-CTLA Control Box (2) P2-CTLB Control Box (3) SMZ25 Zooming Body (4) SMZ18 Zooming Body (5) P2-EFLM Motorized Epi Fluorescence Attachment (6) P2-EFLI Epi Fluorescence Attachment (7) P2-RNI2 Intelligent Nosepiece

 The drive signals of devices (3) and (5) are relayed through

device (1) or (2). For devices (4), (6), and (7), address information signals are relayed.

11.4

For systems where the P2-RLY Relay Box has not been connected, the P2-RLYC Relay Cable is used to connect the SMZ18 Zooming Body to the RELAY connector of the P2-CTLB

P2-RLYC Relay Cable

To the SMZ18 Zooming Body

Control Box. This enables the status detection signals to be relayed.

Viewed from arrow A

(Connect to the USB port of a personal computer or the DS-L3.)

LEDDIA (Connect to the LED light control terminal of the P2-DBL LED Diascopic Illumination Base using the cable supplied with the control box.)

STAND (Connect to the P2-MFU Motorized Focus Unit or the P2-RLY Relay Box.)

DC24V IN (Connect to the 24 V AC adapter.)

ZOOM (Connect to the SMZ25/SMZ18 Zooming Body.)

P2-RLY Relay Box

P2-RLYC Relay Cable

To the STAND connector of P2-CTLA Control Box or the RELAY connector of P2-CTLB Control Box

FL (Connect to the P2-EFLI/ P2-EFLM Epi Fluorescence

ttachment.)

NP (Connect to the P2-RNI2 Intelligent Nosepiece.)

To the RELAY connector of P2-CTLB Control Box

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Chapter 4 Functions and Operations of the Devices

☆☆☆

Page 85

Chapter

Assembling

This chapter contains the SMZ18/SMZ25 system configuration diagrams and lists of the system components, and explains how to assemble the systems.

Before starting assembling, read the appropriate notes such as product” at the beginning of this instruction manual and “3 Installation location” in “Notes on Handling the Product” in this instruction manual.

CAUTION When moving or carrying the system

The SMZ18/SMZ25 system weighs over 20 kg in total.

When moving the assembled microscopy system, work with at least two people. Put hands in the depression at the front of the base and the rear of both sides to lift up the system. Be very careful not to let fingers be caught when putting it down.

CAUTION “11 Cautions on assembling and installing the

 Tools necessary for assembly

Tools Devices that supply tools

Hex wrench (nominal designation: 2.5) Intelligent nosepiece, P-SXY64 XY Stage

Hex wrench (nominal designation: 3) P-SXY64 XY Stage

Hex wrench (nominal designation: 4) Fiber holder

Hex wrench (nominal designation: 5) P-SXY64 XY Stage

Hex wrench (nominal designation: 6) Focus unit

Hex driver (nominal designation: 2) Focus unit, P-PS32 Plain Stand (fit-in type to the top of the support)

Hex driver (nominal designation: 4) Intelligent nosepiece, Focus mount adapter, Focus mount

Spanner wrench (nominal designation: 10) Fiber holder

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Chapter 5 Assembling

Signal cable ZOOM*

Eyepiece Eyepiece tube

P2 RLYC

relay cable

System Configuration

P2-CI coaxial epi illuminator

(With 1/4 lambda plate for

0.5x and 1x objectives)

P2-CTLB

control box

SMZ18

zooming body

TV tube

adapter

Fiber (C-FLED2 LED light source for fiber illuminator)

SMZ25

zooming body

C-mount

adapter

Epi fluorescense attachment

Signal cable ZOOM*

DS camera

Filter cube

(P2-EFLBF is equipped with 1/4 lambda plate for 0.5x and 1x objectives.)

(With light shielding plate)

P2-FM focus mount adapter

intelligent nosepiece

head

HG fiber (C-HGFI/C-HGFIE HG precentered fiber illuminator )

Signal cable FL*

Signal cable

P2-RNI2

NP*

* Connected with FL, ZOOM

and NP on the P2-RLY relay box or P2-MFU motorized focus unit.

1/4 lambda

SMZ18

zooming

body

P-PS32 plain stand

Objective

plate

P2-FMDN

focus mount

P2-CTLA or P2-CTLB control box

P2-RLY relay box

(Connectors: NP, ZOOM and FL)

P2-FU focus unit

P2-MFU

motorized focus unit

(Connectors: NP, ZOOM and FL)

P2-PB plain base

P2-DBL LED diascopic illumination base

P2-DBF fiber diascopic illumination base

P2-CTLA control box

P2-CTLA or P2-CTLB control box

12V AC Adapter and power cable (Necessary when P2-CTL or P2-CTLB control box is not used.)

Fiber (C-FLED2 LED light source for fiber illuminator)

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Chapter 5 Assembling

Control Box

P2-CTLB

control box

(Connectors: HGFIE, USB, LEDDIA, RELAY, and DC24V IN)

24V AC Adapter and

power cable

P2-CTLA

control box

(Connectors: FOOT, REMOTE, HGFIE, USB, LEDDIA, STAND, and DC24V IN)

Other Illuminators

P2-RC

remote controller

AZ-FSW

foot switch

AZ-PCR

photo release

C-FDF

flexible double arm fiber

illumination unit

ttached to base or stand

C-FIDH

fiber holder

Other Stages (Attached to base or stand)

Stage adapters

P-SXY64 XY stage

for AZ

ttached to 0.5x

or 1x objective

P2-FIR

ring fiber illumination unit

P2-POL

simple polarizing

attachment

Other Devices

P-DF LED dark field unit

(Optional P-DF-LSP light shielding

plate is available.)

ring illumination unit

C-HGFI/C-HGFIE

HG precentered

or 1x objective Polarizer unit: Attached to base

or stand

P2-FIRL LED

fiber illuminator

C-FLED2 LED light source for

fiber illuminator

nalyzer unit: Attached to 0.5x

C-SSL DIA

sliding stage

C-TRS

tilting stage

For details, refer to the instruction manual for other devices.

C-FLED2

LED light source

for fiber illuminator

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Chapter 5 Assembling

Device List

Microscopy system is configured by devices that are marked with “.” For details about the restrictions regarding the configurations, see Chapter 4, “Functions and Operations of the Devices.”

Device Model Name Device Name

Main Body

Focus Unit or Focus Mount

Focus Mount Adapter or Nosepiece

Objective

Base, Stand, Stage

Tube

Eyepiece

Episcopic Illuminator

Diascopic Illuminator

Epi Fluorescence Attachment

Control/Relay Device

Remote Control Device

SMZ18 Zooming Body

SMZ25 Zooming Body -

P2-MFU Motorized Focus Unit

P2-FU Focus Unit P2-FMDN Focus Mount - - 

P2-FM Focus Mount Adapter

P2-RNI2 Intelligent Nosepiece

P2-SHR Plan Apo 0.5X

P2-SHR Plan Apo 1X

P2-SHR Plan Apo 1.6X

P2-SHR Plan Apo 2X

P2-PB Plain Base

P2-DBL LED Diascopic Illumination

P2-DBF Fiber Diascopic Illumination

P-PS32 Plain Stand - -  With an acrylic stage plate

P-SXY64 XY Stage

C-SSL DIA Sliding Stage

P2-TERG 100 Trinocular Tilting Tube

P2-TERG 50 Trinocular Tilting Tube

P2-TL100 Trinocular Tube L

C-W 10XB Eyepiece

C-W 15X Eyepiece

C-W 20X Eyepiece

C-W 30X Eyepiece P2-CI Coaxial Epi Illuminator 

C-FDF Flexible Double Arm Fiber

P2-FIR Ring Fiber Illumination Unit

P2-POL Simple Polarizing Attachment

P2-EFLM Motorized Epi Fluorescence

P2-EFLI Epi Fluorescence Attachment Optional

P2-CTLA Control Box

P2-CTLB Control Box Optional P2-RLY Relay Box   -

P2-RLYC Relay Cable Optional Optional Optional P2-RC Remote Controller  

AZ-FSW Foot Switch

AZ-PCR Photo Release

Objective

Base

Illumination Unit

Attachment

Microscopy

System

under

Episcopic

Illumination

 

  -

  



Optional Optional Optional

  

Optional

- Optional -

- 

 

Optional Optional

Microscopy

System under

Diascopic

Illumination

and Epi-

fluorescence

Illumination



- Optional

Compact

System

Remarks



With an acrylic stage plate

With a transparent glass stage

plate

With a transparent glass stage plate

With a 1/4 lambda plate

Retained by a C-FIDH Fiber Holder

With a light shielding plate

Maximum of four filter cubes

can be attached.

Other devices: The P2-FIRL LED Ring Illumination Unit, P-DF LED Dark Field Unit, C-TRS Tilting Stage and DS cameras are available.

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MADE IN CHINA

Assembly Procedures

The SMZ18 and SMZ25 stereo microscopes are system microscopes that can be configured in a variety of ways.

This chapter describes how to assemble the following three systems.

 Episcopic illumination system (called the “EPI System” in this chapter)  Diascopic illumination + epi-fluorescence microscopy system (called the “DIA + EPI-FL System” in this chapter)  Compact system

This chapter also describes how to assemble the devices that can be added to the basic configuration of each system.

Refer to the preceding section “5.2 Device List” and check the devices to be used before assembling.

3.1

Assembling the EPI System and DIA + EPI-FL System

Attach the base.

ttach the base to be used to the P2-FU Focus Unit or P2-MFU Motorized Focus Unit. The base is attached to the focus unit in the same manner in either case. When configuring the DIA + EPI-FL System, select the dia-illumination base. The following figure shows an example of attaching the P2-DBL LED Diascopic Illumination Base to the P2-FU Focus Unit.

CAUTION Heavy Object

The base and focus unit are heavy. The focus unit is especially heavy so be very careful not to drop it when unpacking or installing. Working with two or more people is recommended.

 P2-FU Focus Unit: Approx. 11 kg  P2-MFU Motorized Focus Unit: Approx. 12 kg

(1) Remove the inner cover (cardboard) holding the

focus unit from the packing box. This inner cover is used when connecting the focus unit and base.

Turn the inner cover over and place it on the desk where the microscope is to be installed. Select a desk and location free from vibration. Remove one of the white polystyrene pieces holding the product at either side, change its orientation as shown in the figure and place it again.

P2-FU

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P2-FU

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Changing the orientation of the polystyrene piece

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(2) Place the focus unit in the recession of the

polystyrene piece as show in the figure.

(3) Hold the base with the two base side positioning

holes aligned with the two positioning pins on the focus unit, screw the four M8 hexagon socket head bolts (supplied with the focus mount) from the bottom of the base and tighten firmly using a hex wrench (nominal designation: 6).

Positioning pins

Fitting the focus unit

Attaching the base by tightening the bolts

(4) Install the attached device on the desk.

 Attaching the stage plate

ttach the stage plate.

Depending on the type of the base, either a transparent glass stage plate or an acrylic stage plate is supplied.

The acrylic plate is black on one side and milky white on the other side. Attach the stage plate with the appropriate side facing up based on the sample to be viewed.

Installing the base

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Glass stage plate handling precautions

Be very careful when handling the glass stage plate. Before attaching the glass stage plate, attach all other components in order to avoid the risk of breaking the glass during assembly.

(1) Loosen the M4 set screw for the stage plate in the

front center of the base using a hex driver (nominal designation: 2).

(2) Tilt the stage plate, fit it into the base, and then

tighten the fixing screw.

Attaching the stage plate by tightening the screw

 Attaching a fiber to the P2-DBF Fiber Diascopic Illumination Base

When using the P2-DBF Fiber Diascopic Illumination Base, connect the fiber to the base.

Insert a hex driver (nominal designation: 2) in the fiber fixing screw hole at the right rear side of the base and loosen the internal fixing screw (M4 set screw). Insert the fiber to the rear side of the base until it reaches the limit

Attaching the fiber by tightening the screw

and tighten the fixing screw.

Firmly insert the other end of the fiber into the fiber attachment hole of the C-FLED2 LED Light Source for Fiber Illuminator. See the instruction manual for the light source for details.

 Connecting a cable to the P2-DBL LED Diascopic Illumination Base

Connect the cable to the connector on the left.

Connect the LED brightness control terminal “CTRL” with the connector “LEDDIA” on the control box using the CTRL cable supplied with the control box.

Connecting the LED dia-illumination

Connect the 12 V AC adapter to the AC adapter input terminal that is used for the dia-illumination power supply for the system without a control box connected.

Beware of overhang of cables

When these cables are connected, the connectors (cables) overhang to the left of the base. Be careful so that they do not interfere with other devices such as a control box or they are not accidentally touched and disconnected while attaching devices or performing observation.

Use the power cord specified in Chapter 8, “2 Performance Properties.”

WARNING Before connecting the power cord

To prevent electric shock, be sure to turn off the power switch (press it to the “” position) at the front of the base before connecting the power cord to the adapter.

INPUT

12VDC ,

3.5A

CTRL

ON OFF

base using a cable

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Chapter 5 Assembling

Attach the intelligent nosepiece or focus mount adapter.

ttach the intelligent nosepiece or focus mount adapter to the focus unit. Both the intelligent nosepiece and focus mount adapter are attached to the focus unit in the same manner. The following figure shows an example of attaching the intelligent nosepiece to the P2-FU Focus Unit.

(1) Remove the two rubber caps from the fixing bolt

holes at the right rear of the intelligent nosepiece (as viewed from the front). Insert the hex driver (nominal designation: 4) and completely loosen the two internal M5 hexagon socket head bolts.

(2) Drop and fit the dovetail groove on the rear side of

the intelligent nosepiece onto the dovetail of the focus unit mount from the top.

Attaching the intelligent nosepiece

(3) Firmly tighten the two fixing bolts using a hex driver

(nominal designation: 4). After tightening the bolts, put the rubber caps back onto the bolt holes.

(4) The cover on the left side (as viewed from the front)

of the intelligent nosepiece is a sliding cover. Removing this cover at this point will facilitate the cable connection and routing in “[4] Connect the signal cables.”

Remove the rubber caps from the two fixing bolt holes at the bottom of the cover, loosen and remove the two internal M5 hexagon socket head bolts using a hex driver (nominal designation: 4), and pull the cover up to remove it.

Firmly tightening the bolts

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 Connecting signal cables

Connect the signal cable of the intelligent nosepiece to the connector “NP” on the motorized focus unit or the relay box. (See “[4] Connect the signal cables.”)

Attach the zooming body.

ttach the SMZ18 or SMZ25 Zooming Body to the intelligent nosepiece or focus mount adapter. Both zooming bodies are attached to the intelligent nosepiece or focus mount adapter in the same manner. The following figure shows an example of attaching the SMZ18 Zooming Body to the intelligent nosepiece.

When attaching to the focus mount adapter

Step (1) is not necessary when attaching the zooming body to the focus mount adapter. Proceed to Step (2).

(1) Detach the objective attachment adapter (dovetail

and ring) attached under the zooming body. Loosen the three M3 hexagon socket head bolts using a hex wrench (nominal designation: 2.5).

Signal cable

(2) Completely loosen the two M5 hexagon socket head

bolts for fixing the zoom body on the right side of the zooming body (as viewed from the front) using a hex driver (nominal designation: 4).

(3) Drop and fit the dovetail groove on the rear side of

the zooming body onto the dovetail of the intelligent nosepiece mount.

0.75

Detaching the objective attachment adapter

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Chapter 5 Assembling

(4) Remove the two rubber caps from the fixing bolt

holes on the right front side of the intelligent nosepiece (as viewed from the front). Insert a hex driver (nominal designation: 4) into the holes and firmly tighten the two zooming body fixing bolts. Afte tightening the bolts, put the rubber caps back onto the bolt holes.

 Connecting signal cables

The signal cable for the SMZ25 comes from the left side of the zooming body. In case of the SMZ18, connect the signal cable supplied with the SMZ18 to the round connector on the left side of the zooming body.

Connect the other end of the cable to the connector “ZOOM” on the motorized focus unit or relay box. (See “[4] Connect the signal cables.”)

Attaching the zooming body

by tightening the bolts

Connect the signal cables.

Connect the cables necessary to detect the device state or to drive the motorized devices.

(1) Connect the signal cables.

The relay box has connectors “NP”, “ZOOM”, and “FL” on the left side (as viewed from the front).

The P2-MFU Motorized Focus Unit has connectors “NP”, “ZOOM” and “FL” on the left side of the unit mount (as viewed from the front).

ZOOM

The following cables are connected to each connector.

Connectors on the relay box

 NP: Connect the signal cable coming from the

intelligent nosepiece (there is no connection to the NP connector if the focus mount adapter is attached).

 ZOOM: In case of the SMZ18 Zooming Body,

connect to the round connector on the left side of

ZOOM

the zooming body using the signal cable supplied with the SMZ18. In case of the SMZ25, connect the signal cable coming from the left side of the zooming body.

 FL: Connect the signal cable coming from the

P2-EFLM Motorized Epi Fluorescence Attachment.

Connectors on the motorized focus unit

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Chapter 5 Assembling

Cable routing

Route the cables before attaching the cover.

(In the figure, even though the relay box is already attached for explanatory reasons, the attachment o the relay box is explained in Step [5])

Bring out the cable from the SMZ zooming body along the notch on the focus unit cover and connect to the connector “ZOOM.” Pass the cable from the intelligent nosepiece through the oval hole on top o the mount, bring it out along the notch on the top of the left side cover, and then connect to the connector “NP.”

When the cable was connected to the motorized focus unit, route the cable as shown in the figure.

Pass the cable from the motorized epi fluorescence attachment through the oval hole at the top of the mount, drop it down, and then connect to the connector “FL.”

Cable connection to the motorized focus unit

(2) Attach the left cover of the intelligent nosepiece.

Route the cable and replace the intelligent nosepiece left side cover removed in Step [2]-(4).

Align and fit the two protrusions on the left side cover to the two notches in the top center of the intelligent nosepiece, insert the two M5 hexagon socket head fixing bolts in the fixing bolt holes at the bottom of the cover and firmly tighten them using a hex driver (nominal designation: 4). After the attachment, cover the bolt holes using the rubber caps.

ZOOM

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MADE IN JAPAN

SMZ25

Cable connection to the relay box

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4N75 INSPECTION EQUIPMENT

SMZ25

MADE IN JAPAN

Attach the relay box. (P2-FU Focus Unit system only)

ttach the relay box to the P2-FU Focus Unit.

(1) The relay box is provided with the box itself with the

attaching arm removed. Assemble the box and arm before attaching.

The orientation of the joint is such that the signal cables come out from the right side (as viewed from the front) with the relay box attached to the focus unit. Align the box with the two attachment arm screw holes and attach it by tightening the M5 hexagon socket head bolts supplied with the relay box using a hex driver (nominal designation: 4).

Assembling the relay box

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Chapter 5 Assembling

E I

N C

HI N

(2) Clamp the relay box cable using the arm clamps.

(3) Place the relay box arms over the focus unit as

shown in the figure, align the screw holes of the arm with the two screw holes at the back of the focus unit, and attach it by tightening the M5 hexagon socket head bolts supplied with the relay box using a hex driver (nominal designation: 4).

Clamps

Clamping a cable

Attaching the relay box

5 .7

8 Z1

JAP N I

DE A

O O Z

-FU

I N

C HI

N A

Tightening the bolts for the relay box

 Connecting the relay cable (when not using a relay box)

Use the P2-RLYC Relay Cable to output the zoom magnification information of the SMZ18 to the P2-CTLB Control Box without using a relay box.

5 7 . 0

Connect the relay cable to the round connector on the left side of the SMZ18, route the wire along the notch of the focus unit cover and connect to the RELAY connector of the P2-CTLB Control Box.

Connecting the relay cable

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Attach the coaxial epi illuminator. (for the EPI System)

 Attaching the fiber

ttach the coaxial epi illuminator to the zooming body.

(1) Completely loosen the M4 set screw for the

illuminator at the top front of the zooming body using a hex driver (nominal designation: 2).

(2) Align and fit the round dovetail at the bottom of the

coaxial epi illuminator with the round dovetail of the zooming body and tighten the fixing screw.

Loosen the M4 set screws for the fiber on the side of the two fiber attachment holes on the rear side of the coaxial epi illuminator using a hex driver (nominal designation: 2). Insert the fiber to the attachment hole until it reaches the limit and tighten the fixing screw.

Firmly insert the other end of the fiber into the fiber attachment hole of the C-FLED2 LED Light Source for Fiber Illuminator. See the instruction manual for the light source for details.

Attaching the coaxial epi illuminator

by tightening the screw

Attaching the LED fiber by tightening the screw

 Attaching the 1/4 lambda-plate

Screw and attach the 1/4 lambda plate supplied with the coaxial epi illuminator to the end of the objective.

In case of using a 0.5x objective, remove the protection ring at the end of the objective, and in case of using a 1x objective, remove the correction glass at the end of the objective before attaching the 1/4 lambda plate.

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Attach the epi fluorescence attachment. (for the DIA + EPI-FL System)

ttach the P2-EFLM Motorized Epi Fluorescence Attachment or the P2-EFLI Epi Fluorescence Attachment to the zooming body. Both epi fluorescence attachments are attached to the zooming body in the same manner.

(1) Completely loosen the M4 set screw for the

illuminator at the top front of the zooming body using a hex driver (nominal designation: 2).

(2) Align and fit the positioning groove at the far end of

the round dovetail at the bottom of the epi fluorescence attachment with the positioning pin at the far end of the round dovetail groove on top of the zooming body, and then tighten the fixing screw.

MADE IN JAPAN

P2-EFLM

CUS

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4N75 INSPECTION EQUIPMENT

MADE IN JAPAN

SMZ25

Attaching the epi fluorescence attachment

Tightening the screw for epi fluorescence

 Connecting signal cables

Connect the signal cable that comes from the bottom of the epi fluorescence attachment to the connector “FL” on the motorized focus unit or relay box. In case of the motorized unit, connect it inside the left cover of the intelligent nosepiece. (See “[4] Connect the signal cables.”)

 Attaching the HG fiber

Loosen the M4 set screw for the HG fiber on the top of the HG fiber attachment hole on the rear side of the epi fluorescence attachment using a hex driver (nominal designation: 2).

Insert the emission side tip of the HG fiber to the attachment hole until it reaches the limit and tighten the fixing screw.

Firmly insert the source side connector to the HG fiber attachment hole of the C-HGFI or C-HGFIE HG Precentered Fiber Illuminator. See the instruction manual for the light source for details.

Attaching the HG fiber by tightening the screw

 Attaching the fluorescent filter cube

ttach the filter cube to the turret in the epi fluorescence attachment. Up to four filter cubes can be attached.

Do not touch the surface of the filter with bare hands

Scratches or fingerprints on the filter surface will degrade microscope images. Handle the filter cube carefully.

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MADE IN JAPAN

attachment

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(1) Remove the filter cube replacement hole cover on

the right side of the epi fluorescence attachment (as viewed from the front). Push it upward to remove.

Removing the filter cube replacement hole cover

(2) Align and insert the dovetail groove of the filter cube

to the internal dovetail until it reaches the limit. The installation positions are numbered from [1] to [4] and are shown on the left inside of the filter cube attachment hole.

Be sure to attach four cubes to the turret.

Attach an empty cube to the position to which no filter cube is attached.

(3) Affix the filter cube indication label.

Labels indicating the type of filter cube are supplied with the epi fluorescence attachment. It is useful to identify the type with the labels. Affix a label indicating the type of attached filter cube to the label attachment position at the top of the filter cube attachment hole. Numbers 1 through 4 are indicated next to the label indents. Affix a label for the attached filter cube to the indent corresponding to

ffixing a label indicating the type of the filter cube

the filter cube address.

(4) Replace the cover of the filter cube replacement

hole.

MADE IN JAPAN

P2-EFLC

Attaching the filter cube

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Chapter 5 Assembling

 Attaching a light shielding plate

When using a focus mount, attach the light shielding plate to the zooming body, and when using an intelligent nosepiece, attach it to the bottom of the intelligent nosepiece.

 Attachment to the zooming body

Fit the ring of the light shielding plate to the periphery of the dovetail attachment adapter attached to the lower part of the zooming body, and firmly tighten the M2.5 hexagon socket head bolt on the rear side of the ring using a hex driver (nominal designation: 2).

 Attaching to the intelligent nosepiece

(1) Separate the ring attached to the light shielding

plate. The ring is attached to the light shielding plate with the two M2.5 hexagon socket head bolts. Remove them using a hex driver (nominal designation: 2).

(2) Align the screw holes of the light shielding plate with

the two screw holes at the bottom front of the intelligent nosepiece and firmly attach the light shielding plate using the two M2.5 hexagon socket head bolts that were used to affix the ring.

Attaching the shielding plate

by tightening the screw

Removing the ring from the light shielding plate

Attaching the shielding plate

by tightening the screws