Nikon LV150, LV150A User Manual
M350 E 05.4.NF.1
Industrial Microscope
ECLIPSE LV150/LV150A
Instructions
Thank you for purchasing the Nikon products.
This instruction manual has been prepared for the users of Nikon’s industrial
microscope “ECLIPSE LV150/LV150A.”
To ensure correct usage, read this manual carefully before operating the instrument.
• It is prohibited to reproduce or transmit this manual in part or whole without Nikon’s
expressed permission.
• The contents of this manual are subject to change without notice.
• Although every effort has been made to ensure the accuracy of this manual, if you
note any points that are unclear or incorrect, contact your nearest Nikon
representative.
• Some of the products described in this manual may not be included in the set you
have purchased.
• Be sure to read the instruction manual for any other products that may be used in
combination with the microscope.
Warning/Caution Symbols Used in This Manual
Although Nikon products are designed to provide you with the utmost safety during use,
incorrect usage or disregard of the instructions can cause personal injury or property
damage. For your safety, read this instruction manual carefully and thoroughly before
using the instrument. Do not discard this manual, but keep it near the product for easy
reference.
In this manual, safety instructions are indicated with the symbols shown below. Be sure
to follow the instructions indicated with these symbols to ensure correct and safe
operation.
Symbol Meaning
Disregarding instructions marked with this symbol may lead
WARNING
CAUTION
to death or serious injury.
Disregarding instructions marked with this symbol may lead
to injury or property damage.
Meaning of Symbols Used on the Equipment
Symbol Meaning
Caution for heat.
This marking on the rear of the lamphouse, and near the
lamphouse clamp screw on the illuminator (LV-UEPI and
LV-UEPI2), calls your attention on the following.
For the symbol position, see pages 10 and 12.
• The lamphouse is very hot during and immediately after
illumination.
• Risk of burns. Do not touch the lamphouse during and
immediately after illumination.
• Make sure that the lamphouse has sufficiently cooled
before replacing the lamp.
1
WARNING
1. Intended product use
This microscope should only be used for microscopic observation. Do not use it for any
other purpose. Do not observe such a large sample as to stick out of the stage.
2. Do not disassemble.
Disassembly may cause malfunction, electrical shock, and/or injury. Any injury or damage
due to such an act will not be warranted. Do not disassemble any part other than those
described in this manual. If you experience any problem with the microscope, notify your
nearest Nikon representative.
3. Read the instruction manuals carefully.
For your safety, carefully read this manual and the manuals provided with the other products
to be used with the system. Be sure to read warnings and cautions at the beginning of each
manual in particular.
When the external light source is used:
When you use the external light source using a mercury lamp or so on, handle the lamp with
extreme caution. Read the manual for the light source carefully and observe handling
precautions.
4. Ratings of power supply
The power circuit in this instrument is rated for AC power supplies of 100 to 240 V, 50/60
Hz. When connecting the instrument to a power line, check that the line conforms to the
voltage and frequency ratings mentioned above.
Use of a power line that does not satisfy the ratings may lead to equipment malfunction or
damage or a fire.
5. Power cord
Use only the supplied power cord. Using the wrong power cord could result in damage or a
fire. Also, connect the microscope to a PE (protective earth) terminal, since the microscope
complies with the electric shock protection class I.
And besides, to prevent electrical shock, always turn off the power switch (flip it to “ ”
side) before connecting or disconnecting the power cord.
For details about the specified power cord, see “VIII. Specifications.”
6. Specified light source
This microscope must be used with a specified light source. The following light source
combinations are specified for this microscope.
● Illuminator:
Nikon LV-UEPI Universal Epi Illuminator (model LV-UEPI) or Nikon LV-UEPI2
Universal Epi Illuminator (model LV-UEPI2)
● Lamphouse:
Nikon LV-LH50PC precentered lamphouse 12V 50W (model LV-LH50PC)
● Lamp:
Nikon LV-HL50W 12V 50W LONGLIFE halogen lamp (model LV-HL50W), or nonNikon 12V 50W SHORTLIFE halogen lamp (model OSRAM HLX 64610, OSRAM
HLX 64611, or PHILIPS 7027)
If you wish to buy these lamps, contact your nearest Nikon representative.
2
WARNING
7. Light source other than the specified ones
To perform the epi-fl microscopy wit the LV-UEPI2 illuminator, the specified light source
brightness may be less than the desired brightness. In this case, a light source other than the
specified ones, an external light source, can be used for the LV-UEPI2.
Use the X-Cite 120 (manual type) or X-Cite 120PC (motorized type) manufactured by
EXFO Electro-Optic Engineering Inc. for the external light source. In particular, when the
LV150A is used for the microscope main body, be sure to attach the X-Cite 120PC to
prevent a flash of light. The X-Cite 120PC must be connected with the LV150A through the
RS-232C cable attached to the light source. When the LV150 is used, either external light
source will work.
Please take note that if a light source other than the specified ones are installed onto this
microscope, this microscope system will not be treated as a TUV/SEMI approved product.
8. Heat from the light source
The lamp and the lamphouse become extremely hot. To avoid burns, do not touch the
lamphouse while the lamp is lit or for thirty minutes after it is turned off.
Furthermore, to avoid the risk of fire, do not place fabric, paper, or highly flammable
volatile materials (such as gasoline, petroleum benzine, paint thinner, or alcohol) near the
lamphouse while the lamp is lit or for about thirty minutes after it is turned off.
9. Air vents
Do not block the air vents on the microscope and lamphouse.
If the air vents are blocked, the temperature of the microscope will raise. And it results in
damage or fire.
10. Ultraviolet light from a light source other than the specified ones
If you use a light source other than the specified ones and that has a mercury lamp or so on,
the light source radiates ultraviolet light that is harmful to the eyes and skin from the
emission port. Direct viewing of light from these lamps may result in snow blindness at a
light case or blindness at worst. To prevent injury, follow the guidelines below.
1) Insert the UV collector lens into the optical path of the microscope unless the
UV excitation light is necessary.
On the illuminator LV-UEPI2, the UV filter automatically enters the optical path when
turning the microscopy selection knob to BF (bright-field) or DF (dark-field). The UV
filter is removed from the optical path when turning the knob to FL1 (epi-fl 1) or FL2
(epi-fl 2).
2) When performing the epi-fl microscopy by using the UV excitation light, attach
the filter cube dedicated to the UV excitation light. And then, if you must see
the objective or its surroundings, be sure to see through the ultraviolet light
shield.
3) Attach the light source to the microscope during use.
Always attach the light source to the microscope when the light source is ready to turn
on. Do not turn on the light source unattached to the microscope, or remove the light
source from the microscope while the light source is lit. When removing the light source
from the microscope, turn off the power to the light source, and then unplug the power
code from the wall outlet.
11. Reflection
Lustrous samples reflect the illumination. Do not observe the illuminated surface of a
sample for a long time because the strong reflection may hurt your eyes. When you use the
illuminator LV-UEPI2, be sure to view it through the ultraviolet light shield.
3
CAUTION
1. Handle the system gently
2. Do not wet the microscope
3. Weak electromagnetic waves
4. Installation location
Components of this system are precision optical instruments. Handle them carefully, and do
not subject them to any shocks.
The precision of the objectives in particular can be adversely affected even by weak shocks.
If the microscope gets wet, a short circuit may cause malfunction or abnormal heating of the
microscope. If you accidentally spill water on the microscope, immediately turn off the
power switch (flip it to the “ ” side) and unplug the power cord from the wall outlet. Then,
wipe away the moisture using a dry cloth or the like. If water gets inside the microscope, do
not use it; instead, notify your nearest Nikon representative.
This microscope emits weak electromagnetic waves. The accuracy of any precision
electronic equipment may be adversely affected if positioned too close. If the microscope
affects TV or radio reception, move the radio or TV farther away from the microscope.
Being a precision optical instrument, the microscope may get damaged or loose accuracy if
it is used or stored under unsuitable conditions. When selecting the installation location,
note the following:
• Avoid a brightly lit location, such as exposed to direct sunlight or directly under a room
light. The image quality deteriorates if there is excessive ambient light.
Always install the microscope with a surrounding clear area of 10 cm or more.
• Choose a location that is free from dust or dirt.
• Choose a flat surface with little vibration.
• Choose a sturdy desk or table that is able to bear the weight of the instrument.
• Do not install the microscope in a hot or humid location.
• Select a layout that allows easy removal of the power cord from the microscope’s AC inlet
in the event of an emergency.
• For details about the operating environment and storage environment, see “VIII.
Specifications.”
• Take enough space around the microscope referring to the layout diagrams on page 6.
• The microscope may be moved by earthquakes. We recommend taking anti-earthquake
measures.
For details about the anti-earthquake measures, see “15. Countermeasures for
Earthquakes” in “IV. Assembly.”
5. Cautions on moving the microscope
• The microscope is a precision optical instrument. Handle it carefully and do not subject it
to a strong physical shock. (In particular, objectives may loose accuracy when exposed to
even a weak physical shock.)
• When moving the microscope, first remove the stage and the lamphouse. Then, securely
hold the microscope by the root of the arm from the back.
(Information) The microscope with the stage, eyepiece tube, lamphouse, and other parts
attached, weighs approx. 20 kg.
• Do not hold the focusing knobs, eyepiece tube, lamphouse, sub-stage, etc., when carrying
the microscope. They may come off and may cause serious injury or malfunction.
• Before carrying the stage, attach the fixing metals to hold the movement of the stage plate.
• Be careful not to pinch your fingers or hands during transportation.
4
CAUTION
6. Cautions on assembling the microscope
7. Cautions on lamp replacement
8. Handing of filter cubes
• Be careful not to pinch your fingers or hands during assembly.
• Scratches or fingerprints on the lens surface will adversely affect the microscope image.
Be careful not to scratch or touch the lens surfaces.
• To prevent burn injury, allow the lamp to cool down sufficiently (for at least 30 minutes
after it is turned off) before replacing the lamp.
• To prevent electrical shock and damage to the microscope, always turn off the power
switch (flip it to the “ ” side) and unplug the power cord from the wall outlet before
connecting or disconnecting the lamphouse.
• Do not touch the glass surface of the lamp with bare hands. Fingerprints or grease on the
bulb surface will reduce the illumination intensity of the lamp. Wipe out any fingerprints
or grease attached to the surface.
• Securely attach the lamphouse cover to the lamphouse after replacing the lamp. Never
light the lamp while the lamphouse cover is open.
• When you dispose of the replaced lamp, do not break it up. Instead, dispose of the used
lamp as special industrial waste or dispose of it according to the local regulations and
rules.
When using the microscope configured with the illuminator LV-UEPI2, a filter cube can be
attached to enable epi-fl microscopy. Note the following precautions for handling a filter
cube.
• Interference filters (in particular, excitation filters exposed to intense light) are subject to
aging. Replace them depending on their total operating hours.
• Filters can change in characteristics under high humidity. To avoid changes in
characteristics and quality, do not use or store filters at high temperatures or high
humidity, or expose them to rapid temperature changes. When not using filters, they
should be stored with a drying agent in desiccators or sealed containers.
• The filters fitted in the nine types of filter cubes listed below have sharper wavelength
characteristics than ordinary filters. However, these filters should be handled with care as
they are applied with complicate coating. In particular, be cautious against wear during
cleaning. (Observe the procedures described in “Cleaning Filters and Lenses” of “VII.
Care and Maintenance.”)
Single-band filter cubes: DAPI, FITC, TxRed, and GFP
Multi-band filter cubes: F-R, F-T, D-F, D-F-R, and D-F-T.
5
LAYOUT DIAGRAMS
100
643
(Stage area)
250
168 305
503
(Stage area)
Eye point
Center of gravity position
6 x 6 STAGE
JAPAN
570.4
80192.6
Center of
gravity position
230
This illustration depicts the LV150A microscope configured with the LV-UEPI illuminator, LVTI3 eyepiece tube, LV-LH50PC lamphouse, and 6x6 stage.
508.5
Center of
gravity position
484
230
26279250
Dimensions are in mm.
6
OPERATING POSTURE
The figure below shows the operating posture that prevents strain on your body.
Choose a workbench and a chair having similar dimensions to those shown in the figure.
The 95th percentile male (Height: 189.5 cm)
510
1269 (The height of the eyepiece height point)
405 (The height of the seating surface)
660
* The height of the eye point is that when one eye-level riser is mounted on the microscope.
* Take at least 610 mm of horizontal clearance for your legs.
The 5th percentile female (Height: 147.5 cm)
510
673
760
Dimensions are in mm.
1244 (The height of the eyepiece height point)
565 (The height of the seating surface)
660
* Take at least 610 mm of horizontal clearance for your legs.
135
673
760
Dimensions are in mm.
7
CONTENTS
Warning/Caution Symbols Used in This Manual .............................................. 1
Meaning of Symbols Used on the Equipment .................................................. 2
WARNING ......................................................................................................... 2
CAUTION .......................................................................................................... 4
LAYOUT DIAGRAMS ........................................................................................... 6
OPERATING POSTURE ....................................................................................... 7
Names of Each Part ................................................................................... 10
1When Configured with the Illuminator LV-UEPI ........................................................... 10
2When Configured with the Illuminator LV-UEPI2 ......................................................... 12
3Rear View ........................................................................................................................ 14
Microscopy................................................................................................. 15
1 Bright-Field Microscopy ................................................................................................. 16
2 Dark-Field Microscopy ................................................................................................... 18
3 Differential Interference Contrast (DIC) Microscopy ..................................................... 20
4 Simplified Polarization Microscopy................................................................................ 22
5 Sensitive Polarization Microscopy .................................................................................. 24
6 Epi-Fluorescence Microscopy ......................................................................................... 25
Operation of Each Part.............................................................................. 26
1 Operation of the Illumination .......................................................................................... 26
2 Filters ............................................................................................................................... 26
3 Coarse/Fine Focus Knobs................................................................................................ 27
4 Eyepiece Tube ................................................................................................................. 29
5 Diopter Adjustment ......................................................................................................... 30
6 Interpupillary Distance Adjustment ................................................................................ 30
7 Field Diaphragm .............................................................................................................. 31
8 Aperture Diaphragm ........................................................................................................ 32
9 Illumination Selection Lever and Microcopy Selection Knob ........................................ 33
10 Stage ................................................................................................................................ 34
11 Motorized Nosepiece Operation ...................................................................................... 35
12 Polarizer Slider ................................................................................................................ 36
13 Lambda Plate Slider (for the LV-UEPI2 only) ................................................................ 37
14 Analyzer Slider ................................................................................................................ 38
15 DIC Slider ....................................................................................................................... 39
16 Filter Cubes for Fluorescence Observation (for the LV-UEPI2 only) ............................. 40
17 Excitation Light Balancer (for the LV-UEPI2 Only) ....................................................... 43
8
Assembly.................................................................................................... 45
1 Attaching the Stage and the Holder................................................................................. 47
2 Assembling the Nosepiece .............................................................................................. 48
3Attaching the Illuminator ................................................................................................ 50
4 Attaching the Lamphouse and Replacing the Lamp ....................................................... 53
5 Attaching the Fiver Adapter and External Light Source ................................................. 54
6 Attaching the Eyepiece Tube........................................................................................... 56
7 Attaching Eyepieces ........................................................................................................ 56
8 Attaching Objectives ....................................................................................................... 56
9 Attaching Eye Level Riser .............................................................................................. 57
10 Attaching Column Riser .................................................................................................. 57
11 Connecting the Power Cord ............................................................................................ 58
12 Connecting the RS-232C ................................................................................................. 58
13 Installing Separately Sold Accessories ............................................................................ 58
14 Anti-static Treatment ....................................................................................................... 58
15 Countermeasures for Earthquakes ................................................................................... 59
External Communications Control........................................................... 60
Troubleshooting ......................................................................................... 66
1Viewing and control systems ........................................................................................... 66
2 Electrical.......................................................................................................................... 69
Care and Maintenance .............................................................................. 70
1 Cleaning Lenses and Filters ............................................................................................ 71
2 Cleaning the Painted, Plastic, and Printed Parts.............................................................. 71
3 Storage ............................................................................................................................. 71
4Regular Inspections ......................................................................................................... 71
Specifications ............................................................................................ 72
9
Names of Each Part
1
When Configured with the Illuminator LV-UEPI
Names of Parts
Vertical tube
Binocular part
T
OU
IN
100
0
0
Eyepiece tube LV-TI3
Illuminator LV-UEPI
Nosepiece
Objective
100
BF
DF
STOP A
.
F
STOP
.
Lamphouse
Filter sliders
“CAUTION for
heat” symbol
Polarizer slider
Analyzer slider
*1
*1
Stage
Power indicator
Main body of
the microscope
This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI
illuminator, LV-TI3 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC
microscopy.
DIC slider
6
x
6 STAGE
JAPAN
Tool holders
*1: For DIC microscopy or simplified polarization microscopy.
*2: For DIC microscopy.
*2
10
Names of Operational Parts
Clamp screw for various adapters
Diopter adjustment ring
Eyepiece
I. Names of Each Part
Optical path selection lever
Field diaphragm open/close lever
Aperture diaphragm
open/close lever
Analyzer slider
*1
Prism
movement
knob
Prism
selection
knob
DIC slider
*2
Fine focus knob
Coarse focus knob
OBJ.
Coarse torque adjustment ring
Nosepiece rotation button (on LV150A only)
Rotates the nosepiece counterclockwise (when seen from above the microscope).
Nosepiece rotation button (on LV150A only)
Rotates the nosepiece clockwise (when seen from above the microscope).
OFF
Brightness control knob
OUT
IN
100
0
0
100
BF
DF
STOP A
.
F
STOP
.
Filter sliders
Field diaphragm centering screw
Polarizer slider
*1
Bright/dark-field illumination
selection lever
Stage coarse/fine movement
selection switch
Stage coarse movement lever
6
x
6 STAGE
JAPAN
Stage fine movement knob
for Y-axis
Stage fine movement knob
for X-axis
Fine focus knob
Coarse focus stopper ring
*1: For DIC microscopy or simplified polarization microscopy.
*2: For DIC microscopy.
This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI
illuminator, LV-TI3 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC
microscopy.
11
2
When Configured with the Illuminator LV-UEPI2
Names of Parts
Vertical tube
Eyepiece tube LV-TT2
Binocular part
UT
O
IN
100
0
100 20
TT2
LV-
JA
P
Illuminator LV-UEPI2
Microscopy selection knob
Ultraviolet light shield
DIC slider
*3
AN
BF
FL2
DF
S
FL1
STOP
.
A
STOP
.
F
Lamphouse
Filter sliders
“CAUTION for heat” symbol
Polarizer slider
Dummy slider
Analyzer slider
*1
*2
*1
Nosepiece
6
x
6 STAGE
Stage
JAPAN
Power indicator
Main body of the microscope
*1: For DIC microscopy, simplified polarization microscopy, or sensitive polarization microscopy.
*2: Lambda plate slider in case of sensitive polarization microscopy.
*3: For DIC microscopy.
This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI2 illuminator,
LV-TT2 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC microscopy.
Objective
Tool holders
12
Names of Operational Parts
I. Names of Each Part
Clamp screw for various adapters
Diopter
adjustment
ring
Eyepiece
Microscopy selection knob
Prism
movement
knob
Prism
selection
knob
DIC slider
*2
Fine focus knob
Coarse focus knob
Optical path selection lever
Field diaphragm open/close lever
Aperture diaphragm
open/close lever
OUT
IN
100
0
100 20
LV-TT2
J
A
P
A
N
BF
FL2
DF
S
FL1
STOP
.
A
STOP
.
F
Filter sliders
Aperture diaphragm centering
screw (on both sides)
Field diaphragm centering
screw (on both sides)
Polarizer slider
Dummy slider
Analyzer slider
*1
*2
*1
Stage coarse/fine movement
selection switch
6
x
6 STAGE
JAPAN
Stage coarse movement lever
Stage fine movement knob
for Y-axis
Stage fine movement knob
for X-axis
OBJ.
OFF
Fine focus knob
Coarse focus stopper ring
Brightness control knob
Coarse torque adjustment ring
Nosepiece rotation button (on LV150A only)
Rotates the nosepiece counterclockwise (when seen from above the microscope).
Nosepiece rotation button (on LV150A only)
Rotates the nosepiece clockwise (when seen from above the microscope).
*1: For DIC microscopy, simplified polarization microscopy, or sensitive polarization microscopy.
*2: Lambda plate slider in case of sensitive polarization microscopy.
*3: For DIC microscopy.
This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI2 illuminator,
LV-TT2 eyepiece tube, LV-LH50PC lamphouse, 6x6 stage, and attachments for DIC microscopy.
13
3
Rear View
“CAUTION for heat” symbol
Connector for connecting
the lamphouse
- High Temperature -
CAUTION !
1.
Do not touch the lamphouse while the lamp is lit.
The surface of the lamphouse becomes hot when
the lamp is on.
2.
Turn off the power and allow the lamp and lamphouse to cool enough before replacing the lamp.
Wait for at least 30 minutes after turning off
the lamp
Use 12V50W HALOGEN lamp only.
3.
HALOGEN 12V50W
LV-LH50PC
JAPAN
652701
LAMP
DC12V 50W
I. Names of Each Part
Caution label
Input voltage indication
ECLIPSE LV150A
100–240V~ 1.2A 50/60Hz
MADE IN JAPAN
510001
SEMI® S2
certified by
TÜV Rheinland
TÜV
including interference that may cause
undesired operation.
This Class A digital apparatus complies with
Canadian ICES-003.
Cet appareil numérique de la classe A est
confirme à la norme NMB-003 du Canada.
Grounding tap (M4)
RS232C
RS232C connector
Power switch
AC inlet
This drawing depicts the ECLIPSE LV150A microscope configured with the LV-UEPI
illuminator, LV-TI3 eyepiece tube, LV-LH50PC lamphouse, and 6x6 stage.
14
Microscopy
This chapter describes the procedures for each microscopy.
This microscope can be configured with two types of illuminators, LV-UEPI or LV-UEPI2. See the
table below for the microscopies available with each illuminator, as well as the optional accessories
required for each microscopy.
● If the microscope has not yet been assembled, see “IV. Assembly” on p.45 first.
● See “III. Operation of Each Part” on p.26 for how to operate each part of the microscope.
Microscope
Bright-field
microscopy
Dark-field
microscopy
Differential
interference
contrast (DIC)
microscopy
Simplified
polarization
microscopy
Sensitive
polarization
microscopy
Procedure
p.16 to 17
p.18 to 19
p.20 to 21
p.22 to 23
p.24
Illuminators
LV-UEPI
LV-UEPI2
LV-UEPI
LV-UEPI2
LV-UEPI
LV-UEPI2
LV-UEPI
LV-UEPI2
LV-UEPI2
Required accessories (optional)
–
BD objective
BD quintuple nosepiece, universal quintuple nosepiece
or motorized universal quintuple nosepiece*
(The standard sextuple nosepiece cannot be used
for dark-field microscopy.)
Polarizer
Analyzer
DIC slider
Universal quintuple nosepiece or motorized universal
quintuple nosepiece*
Objectives marked “LU”
(Objectives marked “LU” are suitable for DIC
microscopy.)
Polarizer
Analyzer
Polarizer
Lambda plate
Analyzer
Epifluorescence
microscopy
p.25
LV-UEPI2
Filter cube
(Up to two cubes can be attached.)
Fluorescence excitation light balance filter (optional)
* For the LV150A only.
15
1
Bright-Field Microscopy
When configured with the LV-UEPI
1. Turn on the power.
Push in.
1
2. Set the microscope for
bright-field microscopy
If accessories for DIC
microscopy (*1 to *3)
are in place, pull them
out of the optical path.
3. Place the sample on the
stage and focus on it.
(p.27)
Binocular eyepiece: 100% (P.29)
Push in.
2
BF (bright-field) (p.33)
Select the
10x objective.
3
On the LV150A,
use the nosepiece
rotation buttons. (p.35)
Lower the stage
as far as it will go.
4
Coarse focus knob
(p.27)
Adjust the
brightness.
5
Brightness control
knob (p.26)
Raise the levers.
6
To fully open the field and
aperture diaphragms.
(p.31 and p.32)
Push in the
NCB11 filter.
T
U
NO
I
0
0
01
0
0
0
1
*1
BF
DF
STOP
STOP A
.
F
*3
*2
6
x
6
S
J
A
T
A
P
A
G
N
E
To compensate
color temperature.
Adjust the
brightness.
ND filter
7
(p.26)
8
(p.26)
Power switch
Adjust to circumscribe
the viewfield.
Field diaphragm (p.31)
Image of field diaphragm
4
4. Adjust the diopter. (p.30)
5. Adjust the interpupillary
distance. (p.30)
6. Change the magnification
and observe the sample.
Hint:
It may be difficult to
focus on a sample with
small contrast, such on a
polished surface. In a
case like this, stop down
the field diaphragm so
that its image can be
seen in the viewfield,
and try to focus on the
rim of the diaphragm
image. When the rim is
in focus, the sample is in
focus just as well.
Select the
10x objective.
1
On the LV150A,
use the nosepiece
rotation buttons.
(p.35)
Finely adjust
the focus.
2
Coarse/fine focus
knob (p.27)
Adjust the
brightness.
3
Brightness control
knob (p.26)
Viewfield
Adjust to 70 to 80% of
the objective’s N.A.
Aperture diaphragm
Objective’s
pupil
T
U
IN O
0
0
01
0
0
0
1
BF
DF
STOP
.
A
STOP
.
F
Image of
aperture
diaphragm
5
(p.32)
Adjust
the brightness.
ND filter
6
x
6
S
J
A
T
A
P
A
G
N
E
6
(p.26)
16
When configured with the LV-UEPI2
1. Turn on the power.
Push in.
1
2. Set the microscope for
bright-field microscopy.
If accessories for DIC or
polarization microscopy
(*1 to *3) are in place,
pull them out of the
optical path.
3. Place the sample on the
stage and focus on it.
(p.27)
4. Adjust the angle of the
binocular part. (p.29)
5. Adjust the diopter. (p.30)
6. Adjust the interpupillary
distance. (p.30)
7. Change the magnification
and observe the sample.
Hint:
It may be difficult to
focus on a sample with
small contrast, such on a
polished surface. In a
case like this, stop down
the field diaphragm so
that its image can be
seen in the viewfield,
and try to focus on the
rim of the diaphragm
image. When the rim is
in focus, the sample is in
focus just as well.
Binocular eyepiece: 100% (P.29)
Turn the
microscopy
selection knob.
2
BF (bright-field) (P.33)
Select the
10x objective.
3
On the LV150A,
use the nosepiece
rotation buttons. (p.35)
Lower the stage
as far as it will go.
4
Coarse focus knob
(p.27)
Adjust the
brightness.
5
Brightness control
knob (p.26)
Select the desired
magnification.
1
On the LV150A,
use the nosepiece
rotation switch.
(p.35)
Finely adjust
the focus.
2
Coarse/fine focus
knob (p.27)
Adjust the
brightness.
3
Brightness control
knob (p.26)
II. Microscopy
Raise the levers.
To fully open the field and
aperture diaphragms.
(p.31 and p.32)
Push in the
NCB11 filter.
T
U
O
N
I
0
0
1
0
0
2
0
0
1
2
T
T
-
V
L
JAP
A
N
BF
FL2
DF
S
FL1
*2
STOP
.
A
STOP
.
F
*3
*1
6
x
6
ST
JAP
AG
AN
E
To compensate
color temperature.
Adjust the
brightness.
ND filter
Power switch
Adjust to circumscribe
the viewfield.
Field diaphragm (p.31)
Image of field diaphragm
Viewfield
Adjust to 70 to 80% of
the objective’s N.A.
Aperture diaphragm
Objective’s
T
U
O
IN
0
0
1
0
0
2
0
0
1
2
T
-T
V
L
JAPA
N
BF
FL2
DF
S
FL1
STOP
.
A
STOP
.
F
pupil
Image of
aperture
diaphragm
Adjust
the brightness.
ND filter
6
x
6
STAGE
JAP
AN
6
7
(p.26)
8
(p.26)
4
5
(p.32)
6
(p.26)
17
2
Dark-Field Microscopy
When configured with the LV-UEPI
1. Mount BD objectives and a BD quintuple nosepiece, universal quintuple nosepiece, or motorized universal quintuple nosepiece.
(p.56 and 49)
The standard sextuple nosepiece cannot be used for dark-field microscopy.
2. Focus on the sample with bright-field microscopy. (p.16)
3. Set the microscope for dark-field microscopy.
Pull out.
1
DF (dark-field) (P.33)
Adjust the
brightness.
2
Brightness control
knob (p.26)
4. Return the microscope to bright-field microscopy.
Push in.
1
BF (bright-field) (p.33)
The field and aperture
diaphragms are fully
opened automatically.
(However, the lever
positions are not
changed.)
T
U
IN O
0
0
01
0
0
0
1
BF
DF
STOP
.
A
STOP
.
F
Adjust the
brightness.
3
ND filter (p.26)
6
x
6
S
J
A
T
A
P
A
G
N
E
The field and aperture
diaphragms automatically return to what they
were before the microscope was set to darkfield microscopy.
T
U
O
IN
0
0
01
0
0
0
1
BF
DF
STOP
.
A
STOP
.
F
Adjust the
brightness.
3
ND filter (p.26)
18
Adjust the
brightness.
2
Brightness control
knob (p.26)
6
x
6
S
J
A
T
A
P
A
G
N
E
II. Microscopy
When configured with the LV-UEPI2
1. Mount BD objectives and a BD quintuple nosepiece, universal quintuple nosepiece, or motorized universal quintuple nosepiece.
(p.56 and 49)
The standard sextuple nosepiece cannot be used for dark-field microscopy.
2. Focus on the sample with bright-field microscopy. (p.17)
3. Set the microscope for dark-field microscopy.
Turn the
microscopy
selection knob.
1
DF (dark-field) (p.33)
Adjust the
brightness.
2
Brightness control
knob (p.26)
4. Return the microscope to bright-field microscopy.
Turn the
microscopy
selection knob.
1
BF (bright-field) (p.33)
The field and aperture
diaphragms are fully
opened automatically.
(However, the lever
positions are not
T
U
O
IN
00
1
0
0
2
0
0
1
2
T
-T
V
L
J
A
PA
N
DF
BF
FL2
BF
DF
FL2
S
S
FL1
FL1
STOP
.
A
STOP
.
F
6
x
6 ST
JAPAN
AGE
changed.)
Adjust the
brightness.
ND filter (p.26)
3
The field and aperture
diaphragms
automatically return to
what they were before
the microscope was
T
U
O
IN
0
0
1
0
0
2
0
0
1
2
T
-T
LV
JAP
AN
BF
FL2
DF
S
FL1
STOP
.
A
STOP
.
F
set to dark-field
microscopy.
Adjust the
brightness.
ND filter (p.26)
3
Adjust the
brightness.
2
Brightness control
knob (p.26)
6
x
6 STAGE
JAPA
N
19
3
Differential Interference Contrast (DIC) Microscopy
When configured with the LV-UEPI
1. Mount objectives marked “LU”, universal quintuple nosepiece or motorized universal quintuple nosepiece, polarizer, analyzer, and
DIC slider. (p.36, 38, 39, 49, and 56)
2. Focus on the sample with bright-field microscopy. (p.16)
3. Set the microscope for DIC microscopy.
1
2
3
Information:
The DIC slider can be
operated to enable various
microscopies, including
sensitive DIC.
4
Push in.
Analyzer (p.38)
Push in and align
the marks.
Polarizer (p.36)
Crossed Nicols
position.
Push in.
DIC slider (p.39)
Select the desired
magnification.
On the LV150A,
use the nosepiece
rotation buttons.
(p.35)
Select “A” or “B”.
5
Rotate the inner knob. (p.39)
Match with
the objective
indication.
CF Plan
10X/
0.30
/0 BD DIC
A
Select the
OUT
IN
100
0
0
100
BF
DF
STOP
STOP A
.
F
interference
color.
Rotate the top
knob. (p.39)
6
Adjust the
brightness.
ND filter (p.26)
6
x
6 STAGE
JAPAN
7
Adjust the
brightness.
Brightness control knob (p.26)
8
4. Return the microscope to the bright-field microscopy.
Pull out.
1
Analyzer (p.38)
Pull out.
2
Polarizer (p.36)
Pull out.
3
DIC slider (p.39)
Adjust the
brightness.
4
Brightness control
knob (p.26)
20
OUT
IN
100
0
0
100
BF
DF
STOP
STOP A
.
F
Adjust the
brightness.
5
ND filter (p.26)
6
x
6 STAGE
JAPAN
II. Microscopy
When configured with the LV-UEPI2
1. Mount objectives marked “LU”, universal quintuple nosepiece, polarizer or motorized quintuple nosepiece, polarizer, analyzer, and
DIC slider. (p.36, 38, 39, 49, and 56)
2. Focus on the sample with bright-field microscopy. (p.17)
3. Set the microscope for DIC microscopy.
Push in.
1
Analyzer (p.38)
Push in and align
the marks.
2
Polarizer (p.36)
Crossed Nicols
position.
Push in.
3
DIC slider (p.39)
Information:
The DIC slider can be
operated to enable various
microscopies, including
sensitive DIC.
4. Return the microscope to bright-field microscopy.
Select the desired
magnification.
4
On the LV150A,
use the nosepiece
rotation buttons.
(p.35)
Pull out.
1
Analyzer
(p.38)
Pull out.
2
Polarizer (p.36)
Pull out.
3
DIC slider (p.39)
Select “A” or “B”.
5
Rotate the inner knob. (p.39)
Match with
the objective
indication.
UT
O
IN
100
0
0
2
100
2
-TT
LV
J
APAN
BF
FL2
DF
S
FL1
STOP
.
A
STOP
.
F
CF Plan
10X/
0.30
/0 BD DIC
Select the
interference
color.
Rotate the top
knob. (p.39)
A
6
Adjust the
brightness.
ND filter (p.26)
6
x
6 STAGE
JAPAN
Adjust the brightness.
Brightness control knob (p.26)
T
U
O
IN
0
0
1
0
0
2
0
0
1
2
T
-T
V
L
JAPAN
BF
FL2
DF
S
FL1
STOP
.
A
STOP
.
F
Adjust the
brightness.
ND filter (p.26)
6
x
6 STAGE
JAPAN
7
8
5
Adjust the
brightness.
4
Brightness control
knob (p.26)
21
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