Nikon LABOPHOT-POL Instructions Manual

Nikon

Polarizing Microscope

LABOPHOT -POL

INSTRUCTIONS

NIPPON KOGAKU K.K.

CAUTIONS

oAvoid sharp knocks l

Handle the microscope gently, taking care

to avoid sharp knocks.

_When carrying the microscope

When carrying the microscope, hold its arm
with one hand, supporting the bottom of
the microscope base with the other. The

instrument weighs about 8 kg.

8Place for using

Avoid the use of the microscope in a dusty

place, where it is subject to vibrations or
exposed to high temperatures, moisture or
direct sunlight.

oPower source voltage

..,...ForEuropean districts only-

Make sure of the power source voltage,

220V or 240V, by means of the input

voltage change-over switch wh ich is on the

bottom of the microscope base.

oExchanging the lamp bulb and fuse

Before replacing the lamp bulb (6V-20W)
or fuse, turn OF F the power switch and

disconnect the plug of the power source

cord.
In such cases as of replacement, do not

touch the lamp bulb with bare hands, im-

mediately after putting out the lamp.

(it Dirt on the lens

Do not leave dust, dirt or finger marks on
the lens surfaces.

They will prevent you from clear observa-
tion of the specimen image.

2

~Strain-free glasses

The optical elements of this microscope
being constructed of strain-free glasses,
ta ke particular caution in hand Iing the
objectives and condenser lenses not to

cause strain to them.

8Focus knobs

Never attempt to adjust the tightness of

the right- and lefthand focus knobs by
turning the one, while holding the other in
this model microscope, because of causing

disorder.

CARE AND MAINTENANCE

oCleaning the lenses

To clean the lens surfaces, remove dust
using a soft hair brush or gauze. Only for

removing finger marks or grease, should
soft cotton cloth, lens tissue or gauze
lightly mo istened with absolute alcohol
(methanol or ethanol) be used.

For cleaning the objectives and immersion
oil use only xylene.
Observe sufficient caution in handling
alcohol and xylene.

f)Cleaning the painted surfaces

Avoid the use of any organic solvent (for

example, th inner, ether, alcohol, xylene
etc.) for cleaning the painted surfaces and
plastic parts of the instrument.

8Never attempt to dismantle!

Never attempt to dismantle the instrument
so as to avoid the possibility of impairing

the operational efficiency and accuracy.

OWhen not in use

When not in use, cover the instrument with
the accessory vinyl cover, and store it in a
place free from moisture and fungus.

It is especially recommended that the
objectives and eyepieces be kept in an
airtight container containing desiccant.

oPeriodical checking

To maintain the performance of the instru-
ment, we recommend to check the instru-
ments periodically. (For details of this

check, contact our agency.)

3

CONTENTS

I. NOMENCLATURE 4
II. ASSEMBLy 6
III. PREPARATION 8

1. Interpupillary Distance Adjustment 8

2. Diopter Adjustment 8

3. Optical Path Change-over in the
Trinocular Eyepiece Tube "TP" 8

4. Centering the Objectives 8

5. Centering the Condenser Lens 9

6. Orientation of the Dia-polarizer 9

IV. MICROSCOPY 10

1. Operating Procedure 10

2. Manipulation of Each Element 11

1) Focusing 11

2) Condenser aperture diaphragm 11

3) Field diaphragm 11

4) Circular graduated stage 11

5) Objectives 11

6) Eyepieces 12

7) Achromat strain-free condenser 12

8) Bertrand lens 12

9) 1/4

A & tint plate 13

10) Dia-polarizer and analyzer _ 13

11) Filters 14

12) Illumination system 14

V. PHOTOMiCROGRAPHy 15
VI. ACCESSORIES 17

1. 5enarmont Compensator 17

2. Quartz Wedge 17

3. Monocular Eyepiece Tube "AP" 18

4. Epi-illuminator "M" 18

5. Attachable Mechanical Stage Type "E". 19

VII. TROUBLE SHOOTING TABLE 20
REFERENCE 23
ELECTRIC SPECIFICATIONS 23

I.NOMENCLATURE

Binocular eyepiece tube "BP"

Interpupillary distance scale

Diopter ring

CF eyepiece

Eyeguard

Bertrand lens flip-in/out ring

Centering nosepiece

CF Achromat P objective

(strain-tree)

Circular graduated stage

Circular graduated stage
clamp screw

Vernier

Achromat strain-free condenser

Filters

(B& GIF)

Dust cap

4

Eyepiece tube clamp screw

Analyzer in/out knob

Intermediate tube

clamp screw

Coarse focus knob

Fine focus knob

Orientation plate

Filter receptacle

Fig. 1

Analyzer rotation ring

Intermediate tube "P"

Analyzer clamp screw

Condenser focus knob

Lamp socket

Field diaphragm

control ring

V-POL stand

5

Compensator slot

1/4

A & tint plate

Nosepiece centering screw

Specimen clip

Stage rotation clamp screw

Condenser centering screw

Condenser aperture

diaphragm control ring

Dia-polarizer

Condenser clamp screw

Field lens

Brightness control dial

(including power switch)

Fig.2

II. ASSEMBLY

1/4 A & tint plate

Remove the screw by the side of

the 1/4

A plate of the 1/4 A & tint

plate and insert it into the com-
pensator slot of the intermediate
tube "P", facing the positioning
groove toward the operator side.

Reattach the removed screw.

®

®

CF Achromat P objective

Mount the objectives on the nose-
piece in such positions that thfilir
magnifying power increasesasthe
nosepiece is revolved clockwise.

Input voltage change-over

switch
(For European districts only)

Make sure of the power source
voltage, 220V or 240V, by means
of the input voltage change-over
switch on the bottom of the
microscope base_

• To assemble the microscope, follow the procedure in the order given:

--'

CF eyepiece _

®Insert the eyepiece CFW 10XCM- '"

into the right-hand sleeve, fitting
the pin of the eyepiece in the
right-hand notch of the sleeve.

Into the left-hand sleeve, insert

the CFW lOx.

Bottom of the Base

CD

@2 Achromat strain-free condenser

Insert the condenser into the con-

denser carrier, fad ng the aperture
number plate toward the operator.
Fasten the clamp screw on the left
side of the carrier.

Aperture number plate

•.----

Specimen clip

Place the clip on the stage using
holes on the stagesurface.

Stage clamp screw

Circular graduated stage

Releasing the stage clamp screw
sufficiently, mount the stage on

the circular dovetail of the sub-

stage. Clamp the screw.

Dia-polarizer

After centering the objec-
tives and condenser, insert
the dia-polarizer into the
bottom of the condenser.

@

@)

@

Leveling foot screw

For stable installation of the
microscope, manipulate the adjust-
ing screw at one foot on the

bottom of the microscope base.

6

•

---------. ?:=:

Y·POL stand

@T:.er

(]) Eyepiece tube

Attach the eyepiece tube on the intermediate tube "P", fitting

the notch of the circular dovetail on the end of the clamp screw.
Fasten the clamp screw.

Eyepiece tube clamp screw

®Intermediate tube "P"

Attach the intermediate tube "P" on the arm of the V-POL stand,
fitting the notch of the circular dovetai I on the end of the
clamp screw. Fasten the clamp screw.

Intermediate tube clamp screw

Halogen lamp bulb (6V-20W)

Insert the lamp bulb with its pins

into the accepting holes in the
socket.
Note Don't touch the bulb sur-

face directly with the fin-
ger.

Power source cord

t4'Lamp socket~ Insert the socket into the recep-

tacle on the rear of the base.

Place the filter on the field
lens.

<p = 45

Fig. 3

7

III. PREPARATION

3.. Optical Path Change-over in the 1
Trinocular Eyepiece Tube "TP"

(Fig. 6)

Fig. 8

Vertical photo

tube: 86%
Observat ion

tube: 14%

Optical path
change-over knob

Fig. 7

Fig.6

'* Since the CF eyepieces are of high eye-

point type, it is not necessary for the user
putting on his spectacles to remove them.
Only fold down the eyeguard rubber.

(Fig.8)

I. 4. Centeringthe Objectives

1) Place the specimen on the stage, and focus
on the specimen. Bring an appropriate tar-
get to the center of the cross lines in the
eyepiece.

2) Insert the centering tools in the centering
screws on the nosepiece. (Fig. 9)

(For binocular observation)

1) Mount the specimen on the stage. Swing
the objective 10 x into position, and bring
the specimen image into focus looking into
the right-hand eyepiece.

2) Without manipulating the coarse-and-fine
focus knob, turn the diopter ring on the
left-hand eyepiece to focus on the speci-

men.

Fig. 5

____ J

Fig. 4

2. Diopter Adjustment

Rotate the diopter ring on the eyepiece CFW
10X CM until the cross lines are seen clear.

(Fig.5)

1. ·Interpupiflary DistanceAdjustment I

Place a specimen on the stage, and focus on the
specimen.
As shown in Fig. 4, adjust the interpupillary
distance, so that both the right and left view-
fields become one.

Fig.9

8