Nikon LABOPHOT2-POL Instructions Manual

Thank you very much for purchasing a N ikon Microscope. This microscope is a high precision instrument with a very delicate structure and varied functions. Please thoroughly read this manual first to use the microscope correctly.

CAUTIONS

DAvoid Strong Shocks!

Handle care

the

microscope

to

avoid strong shocks.

gently.

taking

fJWhen Carrying the Microscope

When

carrying

the

bottom Instrument hold

the

microscope,

of

the

microscope

weighs

overhanging

about

7.

portion

support

base. The

9kg.

Do

of

the

IiJPurpose

Use

the

microscope observations. applications.

Do

not

only

use It

for

microscopIc

for

any

mPlace of Use

Avoid

exposing

where

it vibrations, or

direct

may

be

high

sunlight.

the

microscope

subject

temperatures,

to

dust,

to

place

mOisture

mlnstallation

I nstall

the such a way label

IS

vIsible.

microscope

that

the

apart

lamp

unit

from

warning

wall

G)lnput Voltage

Confirm that the

scope

corresponds

BLight

Source

Use a

6V~30W

use

lamps

p.41

(Electrical

of

more

used,

the

damaged.

than

Input

halogen

other

than

Specifications).

the

suggested

light

adjusting

voltage to the

to

your

line

lamp

the

one

wattage

circuit

mlcro~

voltage.

Do

specified

If a

may

not

base.

other

In

not

lamp IS be

(;]When the Lamp

Take care or

bring gasoline. some very

not

Inflammable

thinner

parts

hot.

of

to

the

mChanging the Lamp-bulb

Before replaCing the

the

main

switch source halogen

cool

With

mJDirt

Do

the

prevent clearly.

plug.

enough

your

on

not

leave

lens

or

you

Before replaCing

lamp

(6V~30W),

bare

the

Lens

dust,

bulb

from

IDFocus Knobs

Never

on

the

rlght~

turning

may

cause coarse limits

attempt

focus

since

to

and

left~hand

one

while

problems.

knobs

that

Is

On

touch

the

substances

and

alcohol

lamp

hOUSing

Lamp~bulb,

and

disconnect

Do

not

touch

hands.

dirt

or

surfaces.

obserVing the

adjust

the

focus

holding

Avoid

to

turn

may

cause

lamp

housing

near

become

the

be sure

the

finger

marks

They

will

specimen

tightness

knobs

the

other.

forCing

past

their

problems.

such

as

It, as

turn

off

power

that

It is

glass part

on

of

by

It

the

CONTENTS

I

.NOMENCLATURE

························1

II.ASSEMBLY····································4

III. MiCROSCOPy··························· 11

N .OPERATIONS IN DETAIL .........

1 . Orientation

2.

Focusing and Centering the

Bertrand

3.

SWing

Condenser

4.1

!4A

5.

Centering the Objectives

6.

Stage Rotation .... · ........................

7.

Focusing

8.

Centering the Condenser

9.

Use

Diaphragm

of

the Dia·polarizer

Lens'

.............................

Out

the

Top

Lens of the

.................................

& Tint Plate

....................................

of

Condenser Aperture

........................

.................................

...............

......

..

·19

·20

17

1 8

19

20

21

22

23

10.

Use

of

Field

Diaphragm

..............

1 1 . Optical Path Change· Over ........ ·

12

. Interpupillary

13.

Diopter

14.

Oil Immersion

15.

Brightness Adjustment

16.

Objectives and Eyepieces

DistanceAdjustment·25

Adjustment

and

...............................

.....................

Manipulation

Contrast

........

............

V .OPTIONAL ACCESSORIES ......

1 .

Senarmont

2.

Quartz

3.

Hole Eyepiece

4.

Attachable

5. Universal Epi-illuminator

Compensator

Wedge

Mechanical

..............

..............................

........................

Stage"

.......

...............

VI.TROUBLESHOOTING ...............

ELECTRICAL SPECIFICATIONS

...

..

·30

·24

25

·26

27

29

30

31

32 32

33 35 41

CARE

DCleaning the

To clean the lens surfaces. remove uSing a soft brush removing soft

cotton

moistened

lightly (methyl alcohol

cleaning the objectives use

only xylene.

cleaning the surface of

the eyepiece

of

the

Ultra-Wide

Observe sufficient caution alcohol and the ON-OFF

sWitch.

and xylene (they are

finger

cloth,

Lenses

or

marks

lens tissue,

With pure alcohol

or

ethyl

Do

tube

Eyepiece Tube

of

AND

gauze. Only

or

grease, use a

or

alcohol).

of

immersion

not

use xylene

of

the entrance lens

or

the prism surface

In

handling

inflammable),

the

power

source

gauze

"UW".

fjCleaning the Painted Surfaces

Avoid the

example,

cleaning the painted surfaces and plastic

parts

you use the Silicon

of

the

use

of

any organic solvent (for

thinner,

ether, alcohol)

instrument.

cloth.

We

for

recommend

8lNever Attempt to Dismantle!

Never attempt to dismantle the

instrument

functions.

because you may

impair

MAINTENANCE

dust

when

For

for

the

oil

mWhen

When with

it fungus.

that In

desiccant.

GiPeriodic Checking

To maintain the best Instrument, Instrument

details

authOrIZed Nikon

* Please note

"Any defects or damage directly or

indirectly caused by the unauthorized replacement parts performed

will void the warranty.

Not

in

Use

not

in use, cover the

the accessory vinyl cover, and store

in

a place free from moisture and

It

is

especially

the objectives and eyepieces be kept

an

airtight

container

we

recommend

be periodically

of

this check,

as

per

by

unauthorized personnel"

recommended

containing

performance

checked.

contact

distributor.)

your

N ikon

use

Instrument

of

the

that the

(For

your

warranty.

of

and/or

. .

II

I

I . NOMENCLATURE

NOMENCLATURE

•

Intermediate

Nosepiece screw

Centering revolving nosepiece

CF

Achromat

(Stra i n

Specimen Circular

stage

Stage

Condenser screw

Condenser diaphragm

diaphragm

Field

control

tube

centering

-free)

clip

graduated

clamp

centering

aperture control

ring

P objective

screw

ring

Binocular

eyepiece Eyepiece

clamp

Bertrand lens centering

Analyzer

Intermediate

clamp

screw

Revolving nosepiece

clamp

screw

Coarse-focus adjustment

Fine-focus

Lamp

tube tube

screw

knob

tube

torque

ring

scale

hOUSing

Arm

rest

1

Power

switch

Brightness

adjuster

Fig.1-1

II.

ASSEMBLY

To

assemble

CD

to

ASSEMBLY

the

@.

For details, read

microscope,

follow

p.5

%@

to

p.

the

procedures

10.

given

from

a®---...6

t

~CV

@

Fig.2

~Ptionall

Tools: hexagon

screwdriver,

wrench

coin,

(accessory), plus

etc.

screwdriver,

minus

I.

NOMENCLATURE

Eyepiece

Bertrand lens centering screw

Analyzer

Analyzer rotation ring

Analyzer scale

Coarse

Fine

Power cord

Condenser

knob

Condenser

screw

clamp

focus

screw

knob

focus

clamp

Diopter

Bertrand lens turret

Bertrand

lens

1/4'" & tint

Stage rotation clamp screw

Condenser

swing~out

ring

focus

ring

plate

Vernier

top

lens

knob

(Optional)

N D

filter knob

Fig.1-2

2

II.

ASSEMBLY

CD

Leveling Foot Screw

•

For

stable installation microscope, adjustment rear-right (Fig.2-1).

(2)

Lamp and Lamp Housing

manipulate

screw

of

the

at

base

of

the

I CAUTIONS I

• Use

the

specified

e Use

the

specified

on

p.4 1 tor

• Keep

• Remove buttons

elnsert

(Fig.

• Reattach

bulb,

If

the

wipe

cover

the

2-3).

the

so

bulb

them

after

the

(Fig.

lamp

the

lamp

as

surface

off

mounting

lamp

2-2).

cover.

Cautions when dismounting

• Turn

•

oft

the lamp-bulb Wait

until it

Power

lamp

supply

hOUSing

power

Immediately

cools

cord

is

lamp Halogen

details .

cover

not to touch the surface directly with your fingers.

IS

with

lens

housing

Into

the

socket

switch.

after

enough

should

hot.

"Nikon

on

or

contaminated

tissue.

the

cover

(Assure

turning

for

be kept

Halogen

Lamp.

use gloves

Be sure

lamp.

pressing

pin holes

that

It

replacing.

away

6V

30W".

See Electrical

when

mounting

with

finger to

remove

the

cover

until

it reaches

It is

off.)

Do

off

because

trom

lamp

Specifications

the

marks

It is very

or

the

lamp

detaching

the

not

touch

housing

dirt,

limit

hot.

while

Fig.2-1

lamp

the

5

Fig.2-2

Fig.2-3

Q)

Objectives

•

Screw

such positions

power

in the objectives

that,

when

increases

as

the nosepiece

to

the holes

viewed

Cautions when dismounting I

• Remove If attached and by knob.

• Remove any

the

• Hold drop

the

turning

stage.

the it

when

NO filter cassette

lower

the

coarse

specimen

objective so

unscrewing.

focus

as

stage

from

not

® Stage

•

Lower

• Loosen

• Fit stage

the

stage to the

with

substage by

stage

clamp

circular

the

stage

clamp

turning

screw

dovetail screw ~ (Fig.

from

is

(Ex.)

to

the coarse

sufficiently.

II.

of

the revolving nosepiece in

above,

revolved

of

the

their

clockwise

focus

2-5).

knob.

substage

magnifying

(Fig.

00.

Fasten

ASSEMBLY

2-4).

Fig.2-4

the

@ Specimen Clip

.Insert

(Fig.

the

2-6).

two

speciment

® Condenser

• Raise the substage by

•

Lower

focus

• Insert aperture plate clamp

• Raise focus

the

condenser

knob

.

the

condenser

facing

screw ~ (Fig.

the

condenser

knob.

Fig.2-5

clips into

turning

carrier

into

the

toward 2-7). carrier

the

the coarse

to

its

limit

condenser

the

user. Fasten it

to

its

limit

holes on

focus

by

turning

carrier

by

turning

the

stage surface

knob.

the

condenser

00

with

the

with

the

condenser

Fig.2-6

numerical

condenser

6

II.

ASSEMBLY

(J)

Dia-polarizer

• Fit

the

dia-polarizer

into

Fig.2-7

the

bottom

Fig.2-8

Cautions

• Remove if attached .

•

Lower its

limit condenser Remove releasing screw.

of

the

condensor

when

the

by

the

dismounting

N D filter cassette

condenser turning

focus

condenser

the

condenser

(Fig.

carrier to

the

knob.

by

clamp

2-8).

Fig.2-9

7

@ Intermediate Tube

•

Mount

notch the the L-shaped

the

of

the

clamp

hexgon

intermediate

circular

screw.

wrench.

holder

of

® 1 /4A & Tint Plate

• Remove Into the positioning Screw

the

screw

compensator

notches

in

the

screw

tube

devetail

Tighten

Do

the

wrench.

from

the side

slot

toward

once

Fig.2-10

onto of

the

clamp

not

over

of

the

the user.

removed

the

microscope

intermediate

screw

tighten

(Fig.

of

intermediate

(Fig.

the

2-9)

1/4

A plate. I nsert

2-10).

tube

holding

screw

tube

arm

fitting

with the holding

facing

the

the end

mid-stem

the

plate

the

Fig.2-11

of

@ Binocular Eyepiece Tube

e

Mount of clamp (See @ and

the eyepiece

the

circular

screw.

dovetail

Tighten

Fig.2-11

tube

of

the

.)

onto

the

clamp

I Cautions when clamping I

eTighten

hexagonal optical-path

malfunctioned.

QJ)

Eyepiece

e Use

eyes.

elnsert

by protrusions

e

When

(Fig.2-13).

the eyepiece

wrench.

change-over

the

same

the

eyepieces into

engaging

using eyeguard

the

of

tube

clamp

If overtightened

of

magnification

the

three grooves

the sleeve (Fig.

rubbers,

the

intermediate

eyepiece

screw

the

eyepiece

eyepieces

sleeves

of

the

2-12).

put

them

tube

with

the end

using the hexagon

holding

for

of

the

eyepiece

the stem

the

tube

may be

both

the

binocular

with

onto

the eyepieces

plastic

II.

ASSEMBLY

fitting

right and left

the

of

the

wrench.

of

the

part,

eyepiece

three

notch

tube

*Rotation

The rotation prevent the rotation When

ring

Rotation for

clamp ring for the eyepiece

clamp

removing

and

pull

it out together with the eyepiece.

clamp ring

the

eyepiece

Fig.2-12

ring

is

mounted

of

the

eyepiece.

the

eyepiece, take care not to accidentally hold this

on the eyepiece sleeve

Fig.2-13

to

Fig.2-14

8

II.

ASSEMBLY

The rotation type

of

following

uCaution

Hold

the ring so

There are

prevent

clamp

eyepiece.

ring

When

must

be removed

attaching

points.

when attaching rotation clamp

that

the

¢O.8

protrusion

two

the

pawls

ring

from

on

the

failing

ring

off.

and grooves fits together.

O.

8-diameter

Rotation

for

the

Pawls

clamp

eyepiece

protrusion

ring

Grooves

@ Filter on the Filter Receptacle

Place the (Fig.

2-16).

¢45mm

filter on the filter receptacle

when

the ring

on

and

two

grooves

Attach the

mounting

again,

note on the

ring

the brim faces

on

ring

so

that these pawls

Binocular tube

sleeve

of

the

field lens part

the old

up.

the sleeve

eyepiece

Fig.2-15

to

9

Fig.2-16

@ N D Filter Cassette (optional)

• Push

•

down

cassette field lens When the When

frequently

direction

dismounting,

raise the

the

ND

bottom

part

(Fig.

as

opposite

filter cassette so the

fit

with

the

two

2-17)

.

lowering

shown

the stage,

in Fig.

2-18

push the cassette either

side.

two

mounting

mount

.

Fig.2-17

protrusions

of

the

grooves on the rim

the

ND

filter cassette

to

the

left

or

right

of

te

in

and

II.

ASSEMBLY

Fig.2-18

Fig.2-19

@ Power Cord

e

Connect the receptacle

For

e Use only the

U L No.

el

n case

cord

For220-240Varea------------------------------~

e Use only the

H05VVH2-F,

0625. DIN

e Class I

terminal.

eln

cord

the socket of

base (Fig.

100-120Varea------------------------------~

listed, detachable cord set.

18

AWG

including

VDE

case

including

2-19).

with

the

following

rated a

of

using

PE

3-pole

which

The plug and the

0620

equipment

of

using

PE

the

power

Plug in

ground

minimum 1 25V,

the

extension

wire.

power

must

and DIN VDE

should

the

extension

wire.

conductor

power

supply

be approved

outlet

be

cord

the

other

supply

cord,

0625, connected

cord,

cord set.

3-conductor

use only

cord

are

to

use only the

to

the AC inlet on

end

of

the

(earth

be approved

respectively.

conductor).

grounding

7 A.

the

type

H05VV-F

according

to

PE

(protective earth)

cord

to an AC line

power

or

to

DIN VDE

according

power

the

rear

of

type SVT,

supply

to

supply

@ Hexagon Wrench

e The hexagon

in

the back sta nd . Take it

Flg.2-20.

out

wrench

of

the

as

shown

IS stored

microscope

in

Fig.2-20

10

III.

III. MICROSCOPY

MICROSCOPY

Fig.3-1

Fig.3-2

-""'

...

-----

....

;,,;

, '

" \

:~I

II~/

,

'\

f'MoTe ,

1) Put

2)

3) Turn on

the

NCB

11

necessary

receptacle

(Flg.3-1).

Mount cassette, if used (Fig. (Refer to II.

p.9.)

light Move and line

from

the

lamp.

the

align it

the

filter) on

of

the

optional

ASSEMBLY-

power

brightness

with

right ~ (Fig.

filter

(or

the

filter

field lens part

ND

filter

3-2).

@

switch

the

rn

adjuster

3rd

scale

3-3).

to

11

Fig.3-3

Fig.3-4

4) Pull

the

intermediate

the

analyzer path. turret remove the

optical

analyzer

Turn

to

the

path

knob

of

tube

rn

to

remove

from

the

optical

the

Bertrand lens

position

Bertrand lens

"0" ~ to

(Fig.3-4).

the

from

Fig.3-5

Fig.3-6

Ill.

MICROSCOPY

5)

Move the filter knobs filter cassette bring all

optical path (Fig.

6) Revolve the revolving nosepiece to

swing in the optical the nosepiece properly settles

position (Fig.

7} Place the specimen (glass slide)

on the stage facing two

up

specimen clips (Fig.

to

ND

filters into the

the

path.

3-6).

with

and fasten it

of

the

limit

to

3-5).

10

x objective

Assure

its cover glass

that

with

3-7).

N D

to

in

the

Fig.3-7

Fig.3-8

8} Raise the

using the

3-8).

(Fig.

condenser condenser

uppermost focus

knob

12

Nikon LABOPHOT2-POL Instructions Manual

Specifications and Main Features

Frequently Asked Questions

User Manual