Nikon Labophot User manual

Nikon

Biological Microscope

INSTRUCTIONS

NIKON CORPORATION

CAUTIONS

oAvoid sharp knocks!

Handle the microscope gently, taking care

to avoid sharp knocks.

8When carrying the microscope

When carrying the microscope, hold its arm
with one hand, supporting the bottom of
the microscope base with the other. The
instrument weighs about 8 kg.

oPlace for using

Avoid the use of the microscope in a dusty
place, where it is subject to vibrations or
exposed to high temperatures, moisture or

direct sunlight.

e Light source

Usehalogen lamp 6V - 20W.

o In lighting the lamp

Take care not to touch the rear cover of
the lamp being lighted, and don't bring

inflammable substances such as gasoline,
thinner, and alcohol near to the cover, asit

may take a high temperature while the
lamp is being lighted.

o Focus knobs

Never attempt to adjust the tightness of
the right- and lefthand focus knob by turn-
ing the one, while holding the other in this
model microscope, because of causing

disorder.

o Exchanging the lamp bulb and fuse

Before replacing the lamp bulb (6V - 20W)
or fuse, turn OFF the power switch and
disconnect the plug of the power source
cord.
In such cases as of replacement, do not

touch the lamp bulb with bare hands,
immediately after putting out the lamp.

oDirt on the lens

Do not leave dust, dirt or finger marks on
the lens surfaces. They will prevent you
from clear observation of the specimen
image.

2

CARE AND MAINTENANCE

CONTENTS

oCleaning the lenses

T6 clean the lens surfaces, remove dust

using a soft brush or gauze. Only for
removing finger marks or grease, should
soft cotton cloth, lens tissue or gauze
Iightly moistened with absolute alcohol
(ethyle alcohol or methyl alcohol) be used.
For cleaning the objectives and immersion
oil use only xylene.
For cleaning the surface of the entrance
lens of the eyepiece tube and the prism

surface of the Trinocular Eyepiece Tube
"T" or the Ultra Wide Eyepiece Tube
"UW", useabsolute alcohol.
Observe sufficient caution in handling
alcohol and xylene.

f)Cleaning the painted surfaces

Avoid the use of any organic solvent (for
example, thinner, ether, alcohol, xylene
etc.) for cleaning the painted surfaces and
plastic parts of the instrument.

8Never attempt to dismantle!

Never attempt to dismantle the instrument
so as to avoid the possibility of impairing
the operational efficiency and accuracy.

NOMENCLATURE .

I.

II. ASSEMB LY .

MICROSCOPY .

III.

1. Operating Procedure .

2. Manipulation of Each Element

1) Interpupillary distance
adjustment .

2) Diopter adjustment .

3) Optical path change-over in the
trinocular eyepiece tube 9

4) Centering the condenser lens 10

5) Useof condenser aperture
diaphragm 10

6) Use of field diaphragm 11

7) Focusing 11

IV. OPTICAL SYSTEM .

V. PHOTOMICROGRAPHY 16
VI. USE OF THE ACCESSORIES 19
VII. TROUBLE SHOOTING TABLE 21

1. Optical 21

2. Manipulation 22

3. Electrical 23

4. Photomicrography 23

ELECTRIC SPECIFICATIONS

12

27

4

6

8
8

9

9

9

oWhen not in use

When not in use, cover the instrument with
the accessory vinyl cover, and store it in a
place free from moisture and fungus.

It is especially recommended that the
objectives and eyepieces be kept in an air-
tight container containing desiccant.

oPeriodical checking

To maintain the performance of the instru-
ment, we recommend to check the instru-
ment periodically. (For detai Is of this
check, contact our agency.)

3

I. NOMENCLATURE

Diopter ring

Interpupillary

distance scale

CF eyepiece

Eyeguard

R-revolving nosepiece

Stage Y-axis travel knob

Stage X-axis travel knob

Condenser focus knob

Daylight filter

CF objective

Stage clamp screw

Condenser aperture diaphragm

control ring

Condenser clamp screw

Filter receptacle

Brightness control dial

(Including power switch)

Dust cap

Fig. 1

4

Stage

Binocular eyepiece tube

Eyepiece tube clamp screw

Slide holder

Condenser

Condenser centering screw

Field lens

Field diaphragm control ring

Coarse focus knob

Fine focus knob

Lamp socket

Fuse holder

Power cord

Fig. 2

5

II. ASSEMBLY

• To assemble the microscope, follow the procedure in the order given:

Q)Leveling foot screw

For stable installation of the
microscope, manipulate the adjust-
ing screw at one foot on the bot-

tom of the microscope base.

L

J

®Lamp bulb

Insert the lamp bulb with its
pins into the accepting hole
in the socket.
Note: Don't touch the bulb

surface directly with the

fingers.

,

Pow" C",dl

Q) Lamp socket

Insert the socket into the
receptacle on the micro-

scope base.

/

I

!

iW

•

®Daylight filter

Place the filter on the field
lens. 45 mm in diameter.

6

-----------

®EyepieCe

Insert the eyepieces into the eye-
piece sleevesof the eyepiece tube.

/

@Eyepiece tube

After releasing the eyepiece tube clamp screw
arm, attach the eyepiece tube, and clamp it in

position.

I sufficiently on the right side of the microscope

(]) Objectives

Take stage down by manipulating coarse focus
knob. Mount the objectives on the revolving
nosepiece in such positions that, when, viewed
from the stage-side, their magnifying power in-

creasesclockwise ..

Slide holder

If the microscope is to be used with the lefthand
stage travel knobs, attach the slide holder to the
righthand hole, and if with the righthand knobs, to
the lefthand hole. Slide holder is to be removed

J

when using large specimen.

,,,j.- Stage clamp screw

Releasing the stage clamp screw suffi-

ciently, fit the stage into the circular

dovetail, and refasten the clamp screw.

Aperture number plate

®condenser

Insert the condenser into the condenser carrier,

facing the aperture number plate toward the
user, and fasten the clamp screw.

7

Fig.3

III. MICROSCOPY

I 1.~.,Op~rating~rocedure

1) Turn the brightness control dial (including power
switch) to ON and set the scaleon the dial to 4.

2) Remove the dust cap and place the daylight filter
onto the field lens.

3) Place the specimen on the stage and swing the

10x objective into position. Focus on specimen.

4) Adjust the interpupillary distance and diopter.
(Refer to P.9)

5) Carry out the centering procedure for the con-

denser. (Refer to P: 10)

6) Swi~g in the objective to be used and ~efocuson

specimen.

7) Adjust the condenser. (Refer to Table 1)

Table 1. Useof Condensers

\ Type of

20X

Remove the

Remove the condenser

condenser

condenser

condenser condenser

Abbe

Top lens swung in

Swing-out Achromat

2mm

NA = 1.25

Usable

Top lens swung out

NA = 1.35

1.8mm1,Gmm

Oil-immersion

NA = O,g

Achromat/aplanat

condenser

Dry system

Remove the

Usable

[NOTE] • The above object distance (from the top of the

condenser lens to the specimen surface) includes a
glassslide thickness 1.2mm.

*.When using the Swing-out condenser with 2X or

4X objective, fully open its aperture diaphragm.

• UW (ultra-wide) viewfield observation is possible
with 2X '" lOOX objective. In combination with
the Abbe condenser, however, the use of the lOX
or higher objective is possible.

• For photomicrography using the 4X or lower ob-

jective, remove the Abbe condenser.

• For photomicrography using the 2X objective,
preferably remove the condenser.

• For observation with the 1x objective, additional-
ly usethe diffuser. (available on order).

• The Achromat/aplanat condenser is not included
in the standard set.

8) Brightness is adjusted by changing the lamp
voltage.

9) Adjust the condenser aperture diaphragm and the
field diaphragm. (Refer to P. 10, 11)

8

2. Manipulation of Each Element

1) Interpupillary distanceadjustment

Place a specimen on the stage,and focus on the
specimen. As shown in Fig. 4, adjust the inter-
pupillary distance, so that both the right and

left viewfields become one.

2) Diopter adjustment

Make diopter adjustment for both the right and
lefthand eyepieces.

(1) Turn the diopter ring on each eyepiece,

until the end surface of the milled ring
coincides with the engraved line, as shown
in Fig. 5.

• Repeat the above procedure two times, and
a perfect diopter adjustment will be achiev-

ed.

• The above adjustment, compensating the
diopter difference between the user's right
and left eyes, will keep the tube length of

microscope correct, thus enabling him to
realize the full advantages of the highclass

objectives, including their parfocality.

(4) Since the CF eyepieces are of high eyepoint

type, it is not necessary for the user putting
on his spectacles to remove them.
Only fold down the eyeguard rubber.

(Fig.7)

Fig. 6

Fig. 7

Diopter ring

End surface

Engraved line

Fig. 5

(2) Mount the specimen on the stage. Swmg

the objective 40X into position, and bring
the specimen image into focus. For facilitat·

ing the focusing, first use the

lOX and then

40X objective.

(3) Thereupon, swing the objective 4X mto

position.
Without manipulating the coarse and fine
focus knob, turn the diopter rings on the
eyepieces, so that the specimen images in

the right and lefthand eyepieces are focus-
ed individually

3) Optical path change-over in the trinocular
eyepiece tube

(1) When using the trinocular eyepiece tube

As shown in Fig. 8, when the observation

tube is turned toward the user, 100% of

light enters the observation tube.

Observation
tube 100%

Fig. 8

As shown in Fig. 9, when the observation
tube is revolved 60° leftward, 100% of light
enters the vertical photo tube.

In either case,turn the tube to the limit.

9