Nikon DS-5MC, DS-L1, DS-5M User Manual

M306E 04.10.NF.6 (1/2)

DS Camera Control Unit DS-L1

DS Camera Head DS-5M

DS Cooled Camera Head DS-5Mc

< Instructions >

(Ver. 2.2)

Thank you for purchasing the Nikon products. This instruction manual has been prepared for users of the DS Camera Control Unit DS-L1, DS Camera Head DS-5M, and DS Cooled Camera Head DS-5Mc. To ensure correct use, please read this manual carefully before using these products.

• The contents of this manual may not be reproduced in whole or in part without permission.

• The contents of this manual are subject to change without prior notice.

• Every effort has been made to ensure the accuracy of this manual. If you have any questions

or discover any errors in this manual, please contact your nearest Nikon representative.

• Please refer to the instruction manuals for any other devices you may be using along with these products (e.g., a microscope or personal computer).

Warning and Cautionary Symbols Used in This Manual

Although Nikon products are designed to provide you with the utmost safety during use, incorrect use or failure to observe the instructions may result in injury or property damage. To ensure correct use, read this manual carefully before using your products. Do not discard this manual. Always keep it at hand for easy reference. Safety instructions in this manual are indicated by the symbols shown below. To ensure safety, please follow the instructions indicated by these symbols.

Symbol Meaning

Disregarding instructions indicated by this symbol may result in death or serious injury.

Disregarding instructions indicated by this symbol may result in injury or property damage.

Trademarks Microsoft, Windows, and Internet Explorer are registered trademarks of Microsoft Corporation in the

United States or other countries. Macintosh and MacOS are registered trademarks of Apple Computer, inc. in the United States or other countries. Netscape Navigator is a registered trademark of Netscape Communications Corporation Products and brand names are trademarks or registered trademarks of their respective companies. The “TM” and ® marks are not used to identify registered trademarks and trademarks in these instructions.

- i -

1. Using the equipment

This equipment should be used for photographic purposes only. Do not attempt to use it for any other purpose.

2. Do not disassemble or repair the equipment

The DS-L1 and DS AC adapter contain high-voltage power supplies. Never attempt to disassemble or repair the equipment by yourself; doing so may result in electric shock or equipment failure. Never attempt to disassemble any part of the equipment unless instructed to do so in this manual. If you notice any problems with the equipment, turn off the power and contact your nearest Nikon representative.

3. DS AC adapter

Be sure to use a dedicated AC adapter. Use of any other AC adapter may result in fire or electric shock.

4. Power cord

To prevent electric shock, always turn off the POWER switch on the DS-L1 when plugging in or unplugging the power cord. For the DS-U1, use only the specified power cord. The specifications for the specified power cord are given below. Using another power cord may lead to fire or other hazardous conditions. Because this equipment falls under the category of electric shock protection class I, always make sure it is grounded to a protective ground terminal. If you misplace or damage the power cord, contact your nearest Nikon representative.

• When using the product in 100-120 V AC regions: Use an UL-listed detachable power cord set. 3 conductor grounding Type SVT. No.18 AWG, 3m long maximum, rated at 125V AC minimum

• When using the product in 220-240 V AC regions: Use only an EU/EN-approved three-conductor power cord set. 3 conductor grounding Type H05VV-F, 3m long maximum, rated at 250V AC minimum

5. Handling

• To prevent malfunctions or overheating due to short circuits, do not allow this equipment or any other device used with it to become wet. Never insert any foreign objects or materials into the equipment. If the equipment gets wet, or if foreign matter (e.g., a scrap of paper) gets inside the equipment, turn off the DS-L1’s POWER switch and unplug the power cord from the outlet. Then contact your nearest Nikon representative.

• Do not bend or twist the cables with undue force. Doing so may damage the cables and result in fire.

• Do not place a cloth or paper on the DS-L1 or DS AC adapter. Doing so may cause heat to collect inside the equipment, resulting in fire.

- ii -

1. Installation environment

• Do not use this equipment in locations subject to high temperatures, high humidity, vibration, or excessive amounts of dust. Doing so may result in fire or malfunction.

• When used for an extended period of time, the DS-L1 may become hot. Do not place the DS-L1 on a surface that cannot withstand heat (such as vinyl or plastic). Always install the equipment with a surrounding clear area of 100 mm or more.

• When using the fitting holes on the back of the equipment to install it, always use metal fixtures that can support the weight of the DS-L1 (without directly attaching the equipment to the wall). Leave a clearance of 100 mm or more from the wall surfaces and periphery.

2. Always turn off power when setting up the equipment or when connecting/disconnecting cables.

To prevent damage or malfunction, always turn off the DS-L1’s POWER switch when setting up the equipment or when connecting or disconnecting cables.

3. Connect only the devices specified to the connectors.

To prevent damage to the equipment, connect only the devices specified to the connectors on the DS-L1, the DS Camera Head DS-5M/DS-5Mc, or the DS Remote Controller DS-RC.

4. Cautions on setup, installation, and storage of the equipment

• Be careful to avoid pinching your fingers or hands in the equipment during installation.

• To prevent damage to the equipment, avoid subjecting it to physical shock or violent vibrations during installation.

• If you will not be using the equipment for an extended period of time, unplug the power cord from the power outlet.

•

Store equipment in a location where it will not be subject to vibrations or excessive amounts of

dust.

•

Avoid storing the equipment in hot, humid locations.

- iii -

CONTENTS

CONTENTS.................................................................................................... iv

Components of the Equipment ................................................................... 1

Peripheral Equipment ................................................................................ 2

III

Names of Parts and Their Functions ............................................................ 5

1 DS Camera Control Unit DS-L1 ...................................................................5

2 DS Camera Head DS-5M / DS Cooled Camera Head DS-5Mc................................... 8

3 DS Remote Controller DS-RC (Optional) ....................................................... 8

Connecting and Installing the Equipment..................................................... 9

1 Connection Diagrams ................................................................................ 9

2 Installing the DS-L1 ................................................................................ 11

3 Connection Methods ................................................................................ 12

V Microscope Adjustment ........................................................................... 16

Basic Operations .................................................................................... 19

1 Preparations Before Photographing............................................................ 19

1.1 Using Various Input Devices ...................................................................... 19

1.1.1 Using the DS-L1 Panel Switches ............................................................ 19

1.1.2 Using the DS Remote Controller DS-RC .................................................. 20

1.1.3 Using a Mouse ....................................................................................21

1.1.4 Using a Keyboard................................................................................ 22

1.2 Notes on Using a CF Card .......................................................................... 23

1.3 Menu Configuration and Method of Displaying a Menu...................................24

1.3.1 Menu Configuration .............................................................................24

1.3.2 Method of Displaying a Menu ................................................................25

1.4 Initial Settings .........................................................................................26

1.4.1 Date and Power-save Settings—Additional Settings .................................. 26

1.4.2 Image File Settings—File Settings..........................................................28

1.4.3 Startup Condition Settings—Power On Settings........................................32

1.4.4 Adjusting Monitor Output for an External Monitor.....................................33

1.4.5 Settings for Direct Print........................................................................34

1.4.6 Settings for Connecting a Microscope .....................................................35

2 Photographing Images............................................................................. 36

2.1

Adjusting Camera Operation and Exposure for Better Picture Quality—CAM Menu

2.1.1 Simple Photography—Easy Menu...........................................................36

2.1.2 Special Photography on the Easy Menu—DF/FL Scene Mode ......................40

2.1.3 Fine Adjustment before Photographing—Advn. Menu ................................42

2.1.4 Special Photography on Advn. Menu —Fluorescent Photographing or

Dark Subject Photographing .................................................................53

2.1.5 Special Photography on Advn. Menu – Stroboscope Photographing .............54

2.2 Using a CF Card .......................................................................................55

2.2.1 Saving an Image to a CF Card—REC Menu .............................................. 55

2.2.2 Reproducing Images from a CF Card—VIEW Menu....................................58

..36

- iv -

CONTENTS

2.3 Outputting Images Directly to a Printer.......................................................60

2.3.1 Printing an Image ............................................................................... 60

2.3.2 Various Print Related Settings ...............................................................60

3 Measuring Two-point Distance or Entering Information—TOOL Menu .............. 62

3.1 Outline of the TOOL Menu .........................................................................62

3.2 What is an Overlay? .................................................................................64

3.3 Entering a Comment on the Image ............................................................. 66

3.3.1 Free-form Drawing .............................................................................. 66

3.3.2 Using the Count Marker (to Count the Number of Points) ..........................67

3.3.3 Entering Textual Comments..................................................................68

3.4 Measuring the Distance between Two Points on the Screen ...........................69

3.4.1 Performing Calibration and Saving Values ...............................................69

3.4.2 Calling the Calibration Value ................................................................. 71

3.4.3 Measuring the Distance between Two Points............................................71

3.5 Adjusting the Subject Position and Comparing Sizes .....................................73

3.5.1 Using Crosshairs to Adjust the Subject Position .......................................73

3.5.2 Using a Scale to Measure the Subject Size ..............................................75

3.5.3 Using XY Scales to Measure the Dimensions of the Subject........................76

3.5.4 Creating a Translucent Image and Comparing It to a Live Image................ 77

3.5.5 Using Dual-Window Display to Compare with the Reference Image.............78

4 Using the DS-L1 via a Network ................................................................. 79

4.1 Typical Method of Use...............................................................................79

4.2 Network Settings ..................................................................................... 81

4.2.1 Items Needing Confirmation .................................................................81

4.2.2 Setting Essential Items—Network Settings .............................................. 82

4.3 Operating the DS-L1 from a WEB Browser...................................................85

4.3.1 Setting the WEB Browser ..................................................................... 85

4.3.1(1) When Using Internet Explorer ..........................................................85

4.3.1(2) When Using Netscape .....................................................................87

4.3.2 Using WEB Screens .............................................................................89

4.4 Using FTP Commands to Operate the DS-L1 ................................................96

4.4.1 Using FTP Commands to Get DS-L1 Image Files from a PC ........................96

4.5 Using Telnet Commands to Operate on the DS-L1 ........................................97

4.6 Saving Images to an FTP Server PC from the DS-L1 ..................................... 98

4.6.1 Setting the FTP Server Functions of the PC .............................................98

4.6.1(1) When Using Windows NT Workstation Ver. 4.0....................................98

4.6.1(2) When Using Windows 2000 Professional and XP Professional............... 100

4.6.1(3) When Using Mac OS X................................................................... 103

4.6.2 Setting External Servers of the DS-L1—Network Settings........................ 105

4.6.3 Saving Image Files in the FTP Server ................................................... 107

4.6.3(1) Using the CAPTURE Button to Save Image Files ................................107

4.6.3(2) Using the CAM Menu to Save Image Files......................................... 108

5 Using the DS-L1 when Connected to a PC by a USB Cable..................................110

5.1 Recommended Operating Environment ..................................................... 110

5.2 Connecting the DS-L1 and a PC ............................................................... 112

5.3 Using the DS-L1 in Windows 2000............................................................ 113

- v -

CONTENTS

5.3.1 Installing the Device Driver ................................................................ 113

5.3.2 Outline of the Disk Drive .................................................................... 114

5.3.3 Using the Media Drive........................................................................ 116

5.3.4 Using the CCD Image Drive ................................................................ 117

5.3.5 Removing the DS-L1 from the PC ........................................................ 119

5.4 Using the DS-L1 in Windows XP ...............................................................120

5.4.1 Installing the Device Driver ................................................................ 120

5.4.2 Outline of the Disk Drive .................................................................... 121

5.4.3 Using the Media Drive........................................................................ 122

5.4.4 Using the CCD Image Drive ................................................................ 123

5.4.5 Removing the DS-L1 from the PC ........................................................ 125

6 Using the Microscope Control...................................................................126

6.1 Overview .............................................................................................. 126

6.2 Menu Overview ...................................................................................... 126

6.2.1 Microscope Control Menu 1 ................................................................. 131

6.2.2 Microscope Control Menu 2 ................................................................. 137

6.2.3 Microscope Status Display/Microscopy Setup Menu (MIC Info Menu) ......... 140

6.3 Autofocus Precautions............................................................................. 144

VII

Care and Maintenance............................................................................147

1 Cleaning the System ..............................................................................147

2 Storage ................................................................................................147

VIII

Troubleshooting ....................................................................................148

1 Power Supply ........................................................................................148

2 Image Output........................................................................................149

3 Photographing and Saving.......................................................................151

4 Abnormal Behavior.................................................................................151

5 Networking ...........................................................................................152

6 USB .....................................................................................................155

7 Microscope Control from the DS-L1...........................................................157

Technical Specifications..........................................................................158

1 DS Camera Control Unit DS-L1 ................................................................158

2 DS Camera Head DS-5M .........................................................................159

3 DS Cooled Camera Head DS-5Mc .............................................................160

4 DS Remote Controller DS-RC...................................................................160

5 DS AC adapter.......................................................................................160

6 Overall Specifications .............................................................................160

- vi -

I Components of the Equipment

I

Components of the Equipment

Check to see that all the items listed below are provided in the package. If any items are missing, contact your nearest Nikon representative immediately.

1 DS Camera Control Unit DS-L1

2 Instruction manual

5 DS Camera Head DS-5M or

DS Cooled Camera Head DS-5Mc

C mount cap

Camera cable, 3 m (E229586)

6 DS Remote Controller DS-RC

Remote cable (E120411)

(optional)

3 DS AC adapter

4 Power cord

9 Microscope Control License (optional)

7 Direct Print License (optional)

8 C mount adapter (optional)

- 1 -

II Peripheral Equipment

II

(1) Analog RGB input display

Although the DS-L1 contains a 1,024 × 768 (XGA) 6.3-inch liquid crystal display, you may need an external display if you want to observe images on a larger screen or at a higher 1,280 × 1,024 resolution (SXGA). Prepare an SXGA 60 Hz or XGA 60 Hz TTL-synchronized display available for use with PCs. For best results, we recommend SXGA monitors.

SXGA: 1,280 × 1,024, 60 Hz (non-interlaced) XGA: 1,024 × 768, 60 Hz (non-interlaced)

When shipped from the factory, the equipment is set for XGA output. If you’ve set your equipment to SXGA to start up and are using a display that can only be synchronized to XGA timing, hold down the MENU button on the DS-L1 when powering up. Be sure to hold down the MENU button until the DS-L1 has started up. This forcibly sets the DS-L1 to internal monitor output and external XGA output modes when it starts up. After the DS-L1 has started up, set to XGA in Power On Settings of the SETUP menu and save your settings.

NOTE: If you are using an LCD display or LCD projector, we recommend adjusting

its clock phase and clock pitch.

(2) USB mouse

Peripheral Equipment

You'll need a USB mouse to access functions available only from the mouse (e.g., screen scroll while using the electronic zoom, TOOL functions such as length measurement or the pen drawing function, or histograms). The mouse can also be used for various menu settings and operations. Be sure to use a USB mouse recommended by Nikon. (Not every USB mouse is compatible with

(3) USB keyboard

the DS-L1.)

Although you can enter photographed data and comments using a mouse, it's easier to enter such information with a keyboard. Use a keyboard recommended by Nikon. (Not every USB keyboard is compatible with the DS-L1.)

- 2 -

II Peripheral Equipment

(4) USB hub

You'll need a USB hub to use a mouse, keyboard, and printer at the same time. Use a USB hub recommended by Nikon. (Not every USB hub is compatible with the DS-L1.) Only one hub can be connected to the DS-L1. If your keyboard is equipped with a USB hub, use this hub.

(5) USB printer

Use the specified Mitsubishi CP900D or a printer which supports PictBridge Standard. You must purchase a license to use the printer. Contact your nearest Nikon representative for more information. Restrictions will be displayed on the print screen if you attempt to use the printer without obtaining a license. Refer to “1.4.5 Settings for Direct Print” in Chapter VI for details on how to obtain a license.

(6) CF card

A CF card is needed to record or reproduce photographed data to or from a CF card. We recommend a Type I- or Type II-compliant CF card or CF+ Type II microdrive (1GB).

(7) C mount lens

A C mount lens is required to photograph subjects other than photomicrographs. Any lens that satisfies the following conditions may be used:

• Protrudes no more than 8 mm from the mount surface into the camera.

• The image circle is 2/3". Use a lens for 2/3" or larger (e.g., 1”).

• When using diaphragm settings brighter than F2.8, you may observe slight light falloff

around the periphery.

(8) Network connection cable

You'll need a network connection cable to save image data on another server or to control the DS-L1 from another computer over a network. Use a cable for 100Base-TX (Category 5 or 6). To satisfy EMC standards, use a shielded cable. When connecting the DS-L1 to a LAN, use a straight cable. When connecting the DS-L1 directly to a PC, use a crossed cable.

(9) USB cable

You'll need a USB cable to save image data to a PC or to print directly to a printer via USB. We recommend using a USB 2.0-compliant AB-type cable for connections to a PC and an AB-type cable for connection to a printer.

- 3 -

II Peripheral Equipment

(10) Personal computer (PC)

A PC is required for remote control of the DS-L1 or to handle image data on other media. A PC can be connected to the DS-L1 via USB or over a network. For connect a PC to a network, we recommend the following configuration:

OS: Windows 98/NT/2000/XP Macintosh OS 8.6/9.2/X

Memory: 64 MB or more Browser: Windows Internet Explorer 5.5/6.0

Netscape 6.2/7.0 Macintosh Netscape 6.2/7.0

Although Nikon has confirmed that the DS-L1 operates properly under the above conditions, DS-L1 operations cannot always be guaranteed, since they are affected by OS or browser versions and by various software settings.

(11) Nikon 80i/90i Microscope

If you have a Nikon 80i/90i microscopes equipped with a digital imaging head, you can purchase a license to control the microscope or display its status using the menus on DS-L1. To connect your Nikon 80i/90i microscope to the DS-L1, use the USB cable provided with the microscope. For information on purchasing a license, please contact the nearest Nikon office or distributor.

- 4 -

III Names of Parts and Their Functions

1 DS Camera Control Unit DS-L1

III

1

DS Camera Control Unit DS-L1

Front of the DS-L1

Names of Parts and Their Functions

Internal LCD

1,024 × 768 dots

6.3-inch LCD display

MENU button

Press the MENU button to bring up a menu. After a menu is displayed, the MENU button can be used to confirm a selected menu command, just like the ENTER button.

Cursor buttons

Use one of the four cursor buttons to choose an item within a menu and the MENU button to confirm what you’ve selected.

POWER switch

This is a push switch. Press it to turn on the power. Press again to release it and turn off the power. When the power is on, the LED indicator lights and remains on.

Freeze LED

ZOOM: Electronic zoom button FREEZE: Freeze (pause) button

The freeze LED lights when the display is frozen. CAPTURE: Saves an image to the destination that you selected in

Additional Settings. The destination to which you have saved or printed is indicated by an LED.

CF CARD / PRINT / LAN

- 5 -

III Names of Parts and Their Functions

1 DS Camera Control Unit DS-L1

Left side of the DS-L1

12 V DC output

This output is currently unused.

12 V DC input

Use a dedicated AC adapter.

LAN port

Connect the DS-L1 to a LAN with a LAN cable to exchange data with a PC or server through a LAN.

USB device port

Connect a PC to this port with a USB cable to send and receive data to and from the PC. Or connect a PictBridge-supporting printer to perform the direct print.

USB host port

Use this port to connect a USB mouse, USB keyboard, USB printer (CP900D), or Nikon 80i/90i microsco

Network Tx indicator

hts when sending data to a LAN.

Network Rx indicator

Lights when receiving data from a LAN.

Right side of the DS-L1

Stand arm

Extract the arm to use it.

CF card slot

Insert a CF card into this slot to save or reproduce data to or from the card. * Make sure you're inserting the card in the correct

direction.

- 6 -

III Names of Parts and Their Functions

1 DS Camera Control Unit DS-L1

Rear of the DS-L1

RGB connector

Use this connector to connect an external monitor.

External interface output

Connect a stroboscope or other external device here. This output generates a synchronizing signal when capturing an image, enabling the strobe light to be turned on synchronously. Refer to “3 Connection Methods” in Chapter IV for details on the interface specifications.

Camera connector

Connect the DS Camera Head DS-5M or the DS Cooled Camera Head DS-5Mc to this connector. * Always be sure to turn off the power before connectin

disconnectin Failure to do so may result in malfunction.

External interface input

Connect the DS Remote Controller DS-RC here, and you can perform freeze and capture operations from the DS-RC. A foot switch, etc., may also be used, providing it meets the interface specifications. Refer to "3 Connection Methods" in Chapter IV for details on the interface specifications.

the camera head to or from this connector.

- 7 -

III Names of Parts and Their Functions

2 DS Camera Head DS-5M / DS Cooled Camera Head DS-5Mc

2

DS Camera Head DS-5M / DS Cooled Camera Head DS-5Mc

Tripod mount

This mount can be used to mount the camera head on a tripod, etc.

Top

Rear

Bottom

C mount cap

Protects the C mount from dust.

C mount

When takin the C mount adapter here before connecting to the microscope. When taking other photographs, attach the C mount lens here.

microphotographs, mount

CAMERA OUT connector

NOTE: DS-5M and DS-5Mc have the same

body, only the color differs. DS-5M: White DS-5Mc: Black

3

DS Remote Controller DS-RC (Optional)

To EXT.IN connector

Connect the cable supplied with the unit from this connector to the EXTERNAL IN connector on the DS-L1.

FREEZE button

Freezes the current image as a still picture. To release the frozen image, press the button again.

CAPTURE button

Saves image data. A destination where image data is saved (a CF card, server via FTP, or output directly to a printer) can be assigned. Above functions can be assigned to the button in Additional Settings of the SETUP menu.

- 8 -

IV Connecting and Installing the Equipment

1 Connection Diagrams

IV

Connecting and Installing the Equipment

1

Schematic diagram

PC or a PictBridge-

supporting printer

USB equipment, mouse, keyboard, printer, and Nikon 80i/90i misroscope

Connection Diagrams

LAN

DS-L1

USB cable

LAN cable

CF card

DS AC adapter

DS camera cable

DS-5M/DS-5Mc

- 9 -

RGB cable

DS-RC

IV Connecting and Installing the Equipment

←

1 Connection Diagrams

Connectors

· DS-L1 Left side

USB mouse USB keyboard Printer Microsco

PC or a PictBridgesupporting printer

DS AC ada

LAN ←

USB cable

USB equipment

ter

LAN cable

· DS-L1 Rear

RGB cable

↓

External monitor

Remote cable

↓

DS-RC

Remote cable

↓

DS-5M/DS-5Mc

- 10 -

IV Connecting and Installing the Equipment

2 Installing the DS-L1

2

When installing the DS-L1 on a flat surface such as a desktop by using the stand arm

Installing the DS-L1

Extract the stand arm from the DS-L1 and place it at an angle of 5 to 10 degrees, as shown below. Leave a clearance of about 100 mm around the DS-L1 to prevent heat from collecting near the equipment.

When attaching a commercially available arm to the DS-L1

Any commercially available VESA standard-compliant 75-mm arm, UL1678-suited stand and mount can be attached to the DS-L1. When doing so, make sure the following specifications are met:

DS-L1 weight: 1.3 kg Fitting screw hole: M4, 7-mm deep Tightening torque: 80-120 N·cm

Leave a clearance of about 100 mm around the DS-L1 to prevent heat from collecting near the equipment.

M4 screw holes

Arm

Side view of the DS-L1 Rear view of the DS-L1

- 11 -

IV Connecting and Installing the Equipment

3 Connection Methods

3

Connection Methods

(1) Connecting the DS Camera Head DS-5M

(Equivalent to that for DS-5Mc)

Be sure to turn off the power for the DS-L1 before making any connections. Connect one end of the camera cable supplied with the unit to the CAMERA connector on the DS-L1 and the other end to the CAMERA OUT connector on the DS-5M. Squeeze the locking tabs on the connector as you plug in the connector. When the connector is plugged in all the way, the locking tabs return to their original positions, locking the connector in place.

Mounting the camera head on a microscope

1) Remove the C mount cap from the C mount on the camera head.

2) Make sure there is no dust on the C mount adapter. If there is, use an air blower or similar means to remove it.

3) Screw the C mount adapter into the C mount on the camera head. Screw it in as far as it will go.

4) Insert the C mount adapter into the vertical tube of the microscope. Use the clamping screw on the vertical tube to secure the C mount adapter in place.

To CAMERA connector on the DS-L1

DS-5M

C mount

C mount adapter

Vertical tube of microscope

You can also attach a x0.7 relay lens. When you have mounted the camera head on a microscope, be sure to read "Chapter V Microscope Adjustment" as well.

Installing a third-party C mount lens on the camera head

Select a mountable lens (see "Chapter II Peripheral Equipment"), then screw it into the C mount. Note that the image quality produced by this product is tuned for microphotography. The tripod mount on the camera head can also be used to mount the camera head on a tripod.

(2) Connecting an External Display

Be sure to turn off the power for the DS-L1 and the display before making any connections. Connect one end of the D-sub 15-pin connecting cable supplied with the unit to the RGB connector on the DS-L1 and the other end to the D-sub 15-pin connector on the display. Secure the connections, using a screwdriver to tighten the screws on the cable connectors.

- 12 -

IV Connecting and Installing the Equipment

3 Connection Methods

(3) Connecting USB Peripheral Equipment

Be sure to turn off the power for the DS-L1 before making any connections. When using a USB mouse, USB keyboard, USB printer (Mitsubishi CP900D), or Nikon 80i/90i microscope independently, connect the device directly to the USB-H connector on the DS-L1. When using USB devices simultaneously, connect them to a USB hub. Only one USB hub can be connected to the DS-L1. If your keyboard is equipped with a USB hub, use the hub built into your keyboard.

(4) Connecting to a PC or a PictBridge-Supporting Printer with USB Cable

When connecting to a PC, connect one end of the USB 2.0 cable (AB type) to the USB connector on the PC and the other end to the USB-D connector on the DS-L1. When connecting to a PictBridge-supporting printer, connect the PictBridge-supporting printer and the USB-D connector on DS-L1 with a USB cable (AB type).

(5) Connecting the DS Remote Controller DS-RC

Be sure to turn off the power for the DS-L1 before making any connections. Connect one end of the remote cable supplied with the unit to the EXTERNAL IN connector on the DS-L1 and the other end to the TO EXT.IN connector on the remote controller.

(6) Connecting to a Network

Be sure to turn off power for the DS-L1 and the equipment to be connected before making any connections. In addition, be sure to consult your network administrator. Make the connections as shown below. We recommend using cables and hubs designed for 100BASE-TX use.

Connecting to a LAN

Left side of the DS-L1

To Ethernet connector

Third-party 10/100BASE-TX

(category 5) cable (straight)

Third-party 10/100BASE-TX

(category 5) cable (straight)

Network

Hub

- 13 -

IV Connecting and Installing the Equipment

3 Connection Methods

Connecting to a PC without going through a LAN

Left side of the DS-L1

Third-party 10/100BASE-TX (category 5) cable (crossed)

o Ethernet

connector

(7) Connecting External I/O Devices

Be sure to turn off the power to the DS-L1 and any equipment to be connected before making connections. When an external device is connected to the EXTERNAL IN connector on the DS-L1, the image can be frozen or captured by trigger input from the external device. Furthermore, if an external device is plugged into the EXTERNAL OUT connector, a strobe synchronizing output can be obtained when capturing an image. If you are preparing your own external device, use a device that satisfies the specifications shown below. Connector: 3.5-mm diameter stereo pin jack (EXTERNAL IN connector) Connector: 2.5-mm diameter stereo pin jack (EXTERNAL OUT connector)

Pin 3: GND

Pin 2

Pin 1

EXTERNAL IN

Functions Pin 1: FREEZE Pin 2: CAPTURE You will not be able to capture desired images at precise, split-second moments. Use of this input is not suitable for photographing fast-moving objects using a sensor.

3.3V

3.3kΩ

DS-L1 side

GND

Pin 1 or 2

Activated when the switch is ON (closed) Required pulse width: 10 ms or more

Pin 3

- 14 -

IV Connecting and Installing the Equipment

3 Connection Methods

EXTERNAL OUT

Functions:

Extended external output pins 1 and 2, both for strobe synchronizing output This output is used mainly for synchronized firing of a stroboscope when capturing an image with the DS-L1.

Electrical characteristics of strobe synchronizing output are open-collector output, maximum input current = 10 mA, withstand voltage = 24 V, and internal resistance = 100 Ω. The output pulse width is 67 ms. This output can be connected to external devices that satisfy the following electrical characteristics. (See the diagram below.)

• Strobe synchronizing output is tied to a power supply line of 24 V or less through a pullup

resistor R on the external device side.

• Inputs on the DS-L1 and external user device sides have the same GND potential or are

left floating.

100Ω

DS-L1 side External device side

When plugging a stroboscope or other external device into the EXTERNAL OUT connector of the DS-L1, make sure the power for the external device is turned off. If the power for the external device remains on, your eyes may be damaged by unintended light emission.

(8) Connecting to a Power Supply

Pins 1 and 2

Pin 3

E≤24V

R≤E/10(kΩ)

ON-time level (100xE)/(R+100)(V)

OFF-time level E(V)

To prevent electric shock, turn off the power for the DS-L1 before making any connections. Complete all other connections before plugging into a power supply. When connecting the DS-L1 to a power supply, always use the specified AC adapter (DS AC adapter) to which a dedicated power cord should be connected. Using the wrong AC adapter or power cord may lead to a fire or other dangerous conditions. This product belongs to electric shock protection class I and must be connected to a power outlet with a protective ground terminal. If you misplace or damage the DS AC adapter power cord, please contact your nearest Nikon representative.

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V Microscope Adjustment

V

(1) Use the Appropriate Filters

When the camera head is mounted on a microscope, adjust the microscope as described below.

When capturing a color image:

Insert the NCB filter into the optical path.

When capturing a black & white image:

Insert the appropriate filter into the optical path, depending on the photographic purpose. Generally, inserting a green interference filter (GIF) will heighten contrast. Contrast is enhanced by inserting a filter with a color complementary to the specimen.

Supplement

* The method for inserting and removing a filter varies from microscope to

microscope. Refer to your microscope’s instruction manual.

* A third-party color compensation filter (CC filter) can be inserted into the

illumination optical path of the microscope to compensate for changes in color balance caused by the length of the exposure time.

* When using a phase contrast microscope or an interference microscope (two

luminous fluxes or multiple luminous fluxes), you can enhance contrast using a green interference filter (GIF) or a monochrome interference filter (IF).

* Some microscopes may require a heat-wave absorption filter.

Microscope Adjustment

Terms

What is an NCB filter?

An NCB (neutral color balance) filter is a color-balancing compensation filter used to adjust color temperature to daylight values in microscopes that use a halogen bulb as a light source.

What is a complementary color?

For example, green and magenta, red and cyan, and blue and yellow are all pairs of complementary colors. When complementary colors overlap, their respective hues cancel each other out.

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V Microscope Adjustment

(2) Adjusting the Field Diaphragm

Adjust the field diaphragm so that it circumscribes the viewfield. The field diaphragm has a significant impact on contrast, especially for fluorescent specimens against dark backgrounds. Make sure you adjust the field diaphragm correctly. For details on adjustment, refer to your microscope’s instruction manual.

(3) Adjusting the Aperture Diaphragm

When using diascopic illumination:

Generally, the numeric value of the condenser aperture diaphragm should be adjusted to approximately 70% to 80% of the numerical aperture (N.A.) of the objective.

When using episcopic illumination:

Generally, the aperture diaphragm should be adjusted to approximately 70% to 80% of the size of the pupil of the objective that can be seen by removing the eyepiece and looking into the eyepiece tube.

Supplement

* For details on adjusting the aperture diaphragm, refer to your microscope’s

instruction manual.

* For photographed images that have greater depth of focus, close down the

aperture diaphragm. Note that closing down the aperture may limit the performance of the objective.

Terms

What is depth of focus?

"Depth of focus" refers to the range along the axis of the field of view over which the image is in apparent focus. Although reducing the aperture diaphragm extends the depth of focus, doing so will also reduce resolution. Take this into consideration and adjust the aperture diaphragm in accordance with your needs.

(4) Adjusting the Focus

Adjust the focus of the microscope so that the image can be clearly seen on the display.

(5) Adjusting the Lamp Voltage

When photographing a color image, the color reproducibility of the image depends on the lamp voltage. When a halogen lamp is used as the light source, increasing the lamp voltage produces a bluish light, while decreasing the lamp voltage produces a reddish light. Except in cases where it is specifically necessary to adjust the tone, the voltage should be set to the proper level for microphotography, which depends on the microscope being used. (Since this level varies for each type of microscope, refer to your microscope’s instruction manual.) If the lamp voltage changes, measure the white balance once again.

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V Microscope Adjustment

(6) Adjusting the Exposure Time

Use an ND filter to adjust the illumination for the microscope to a level that yields a suitable exposure time (generally 1/15 to 1/250 s). You can also adjust the camera sensitivity.

Terms

What is an ND filter?

An ND filter is a filter that affects only the amount of light passed, not the color balance of the light. For example, an "ND2" filter cuts transmitted light in half, while an "ND16" filter reduces transmitted light to 1/16th of actual levels.

(7) Effects of Ambient Light

When capturing a dark specimen with a fluorescent microscope, for example, ambient indoor light may enter the optical path. Accordingly, interior lighting should be darkened for photomicrography. Place caps on the binocular eyepiece to prevent light from entering through the eyepiece.

(8) Effects of Vibration

Because photomicrography involves extremely high image resolution, the slightest vibration can affect image quality. To insulate the microscope from vibration, set it on a solid, stable surface on a solid floor. Using an anti-vibration table compatible with the microscope will further reduce the effects of vibration. While actual microphotography is in progress, be careful to avoid touching the microscope or the desk or stand on which it is positioned.

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VI Basic Operations SETUP

1 Preparations Before Photographing

VI

1

1.1

1.1.1 Using the DS-L1 Panel Switches

Preparations Before Photographing

Using Various Input Devices

ZOOM button FREEZE button CAPTURE button

Basic Operations

MENU button

Cursor buttons

MENU button (menu/enter button)

Pressing the MENU button brings up the CAM Easy menu. While the menu is displayed, use this button as the ENTER button to confirm your selection.

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.SETUP. VI Basic Operations

1 Preparations Before Photographing

Cursor buttons

Use these buttons to select an icon in a menu or to increment or decrement a set value.

• For an icon to increment or decrement a value such as the Gain icon on the CAM menu Position the cursor over the icon and press the MENU button to select the icon. The color of the cursor frame changes to blue. Press the right or top cursor button to increment the value or the left or bottom cursor button to decrement the value.

• For a shutter speed The value changes in the same way as described above. The right or top cursor button lowers the shutter speed to increase exposure, whereas the left or bottom cursor button increases the shutter speed to reduce exposure.

• For a mode-select icon, such as one to choose an exposure method on the CAM menu Select an icon you want and use the right or top cursor button to select items in the forward direction or the left or bottom cursor button to select items in the reverse direction.

ZOOM button (electronic zoom button)

Use this button to apply an electronic zoom. The image zooms in or out at the magnification selected for E.Zoom Step in Additional Settings of the SETUP menu.

FREEZE button (freeze button)

This button allows you to switch the state of a photographed image between freeze and live.

CAPTURE button (capture button)

Use this button to save an image. This button can be assigned a save destination (CF card, FTP server, or printer). The image will then be saved to the destination selected for Capture Function in Additional Settings of the SETUP menu.

1.1.2 Using the DS Remote Controller DS-RC

FREEZE button CAPTURE button

FREEZE and CAPTURE buttons

These buttons serve the same function as the FREEZE and CAPTURE buttons on the front panel of the DS-L1.

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VI Basic Operations SETUP

1 Preparations Before Photographing

1.1.3 Using a Mouse

You can use a USB mouse with the DS-L1. Be sure to use a recommended mouse. Not every USB mouse is compatible with the DS-L1. While most normal operations involve left-clicking the mouse, some may require right-clicking. For icons to set a value or select a mode, left- or right-clicking the mouse button allows you to increment or decrement a value or select items in the forward or reverse direction.

• For an icon to increment or decrement a value such as the Gain icon on the CAM menu Left-clicking the icon increases the value, while right-clicking the icon reduces the value.

• For a shutter speed Left-clicking reduces the shutter speed to increase exposure; right-clicking increases the shutter speed to reduce exposure.

• For a mode-select icon, such as one to choose an exposure method on the CAM menu Left-clicking lets you select items in the forward direction; right-clicking lets you select items in the reverse direction.

Some functions are available only with the mouse. You will need a mouse to access all the functions of the DS-L1. The following functions are not accessible without a mouse:

• Electronic zoom scroll

• Screen scroll when split into halves

• Histogram display

• Pen drawing function

• Text input function

• Count marking function

• Two-point distance measurement function (including calibration)

• Superimposed position adjustment

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.SETUP. VI Basic Operations

1 Preparations Before Photographing

1.1.4 Using a Keyboard

You can use a USB keyboard with the DS-L1. For frequent text or character entry, you'll find using a keyboard most convenient. Be sure to use a recommended type of keyboard. Not all USB keyboards are compatible with the DS-L1. Alphanumeric characters in upper- or lowercase can be used with the DS-L1. The function keys on the keyboard can be assigned various functions, such as displaying a menu, freezing or the Capture button for quick operations.

Function key assignments on the USB keyboard

F1 CAM menu F2 VIEW menu F3 REC menu F4 TOOL menu F5 SETUP menu F6 MIC menu F7 F8 F9 E.ZOOM– F10 E.ZOOM+ F11 FREEZE F12 CAPTURE Back Space Deletes one character during character entry SHIFT + Backspace Deletes the entire text during character entry

NOTE: The DS-L1 is factory set to a Japanese keyboard layout. If you wish to use

an English keyboard, issue the Telnet command shown below to change keyboard settings. To revert to the Japanese keyboard, use the Telnet command shown below to change keyboard settings. w_KYBUS Selects an English keyboard w_KYBJP Selects a Japanese keyboard Settings you’ve changed will take effect upon restarting the DS-L1 after executing the command. For information on Telnet connections, refer to “4.5 Using Telnet Commands for the DS-L1” in Chapter VI.

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