Nikon 44100, 44104, 44102, 44106 User Manual

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Table of Contents

Introduction …………………………………………………………………………………. 2

Laboratory Model # 44100 …………………………………………………………………. 3

Laboratory Model # 44102 …………………………………………………………………. 5

Advanced Model # 44104 …………………………………………………………………… 8

Advanced Model # 44106 …………………………………………………………………… 13

Care and Maintenance ……………………………………………………………………… 18

Warranty …………………………………………………………………………………….. 19

Introduction

Congratulations on your purchase of a Celestron microscope. Your microscope is a precision optical instrument, made of
the highest quality materials to ensure durability and long life. It is designed to give you a lifetime of pleasure with a
minimal amount of maintenance.

This instruction manual covers four microscope models. Please be sure to locate your specific model of microscope in
order to ensure you read the correct information.

Before attempting to use your microscope, please read through the instructions to familiarize yourself with the functions
and operations to maximize your enjoyment and usage. See the microscope diagrams to locate the parts discussed in this
manual.

The microscopes described in this manual provide high powers from 40 up to 1000x. These microscopes types are ideally
suited for examining specimen slides of yeasts and molds, cultures, plant and animal parts, fibers, bacteria, etc.

The final section provides simple care and maintenance tips for you to follow to ensure that your microscope provides you
with years of quality performance, usage, and enjoyment.

2

Laboratory Microscope – Model # 44100

10. Arm

9. Safety Rack
Stop

1. Eyepiece

2. Eyepiece
Tube

3. Focus Knob

4. Objective Lens

8. Spring Loaded
Stage Clips

Figure 1

Standard Accessories Included with your Microscope

• 10x Huygens Eyepiece • 5 Prepared Slides

• 20x Objective Lens (200x with eyepiece) • Dust Cover

Specifications

Model # 44100 Specifications

Stage Plain Stage with spring loaded clips -- 88mm x 88mm
Arm Angle choice from 0° to 45°
Focuser Coarse; Rack and Pinion with Safety Stop
Objective Achromatic 20x
Illuminator 42mm Plano-Concave Mirror

Eyepiece 10X -- 12mm Field of View

5. Specimen Stage

6.Illumination
Mirror

7. Base

3

Setting Up Your Microscope

1. Take the Styrofoam container out of the cardboard carton.

2. Remove the tape from the Styrofoam container holding the two sections together.

3. Carefully remove the microscope and other parts and set them on a table, desk, or other flat surface.

4. Remove the plastic bag covering the microscope.

5. Remove the protective paper from the stage (5).

6. Remove the plastic cap from the eyepiece tube (2).

7. Insert the 10x eyepiece in the eyepiece tube (2). Your microscope is now ready to use!

Microscope Operation

Using the Illuminator Mirror

Your microscope has a built-in plano-concave illumination mirror which allows you to illuminate the specimen from the
bottom by reflecting an external light source (indirect sunlight, room light, lamp, etc.) to the stage. The mirror has a flat
side (you can easily see your reflection on the flat surface) and a concave side. The concave side will concentrate more
light onto the specimen than the flat side. To illuminate a specimen:

1. If it is daylight, tilt the mirror (6) and reflect indirect sunlight through the bottom of the stage (5).

use the mirror to focus direct sunlight through the microscope as this can be very harmful and may damage your
eyes.

2. If viewing at night or indoors, away from sunlight, you can use a nearby desk lamp, table lamp, or other light source

to reflect light onto a specimen.

To control the amount of light reflecting onto the specimen:

1. Use the concave side of the mirror to focus more light on the slide. The concave side will concentrate more light onto
your specimen but will focus a smaller beam of light, making it more sensitive to adjust.

2 If you are unable to adjust the mirror enough to properly illuminate the specimen, you can also tilt the arm (10)

backwards to change the position of the mirror.

Adjusting the Arm Angle

The normal viewing position is at 0°. However, you can view at any angle from 0° to 45°. To change the viewing angle:

1. With one hand hold the base (7).

2. Then, with your other hand, tilt the arm (10) by pulling it backward or forward to the desired viewing angle.

Viewing a Specimen

Carefully place a specimen slide under the spring loaded stage clips (8) and center the specimen directly over the hole in the
center of the stage (5).

You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the
specimen slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the
range of travel on the focuser. This assures that the objective lens will not accidentally come into contact with the
specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focus mechanism:

1. Raise the objective lens away from the specimen stage (5) by rotating the focus knob (3) backwards (clockwise ).

2. Unthread the safety rack stop screw (9) about 2/3 of the way.

3. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to

touch the objective lens to the slide).

4. Thread the safety screw (9) upward until it stops against the focus mechanism.

5. Rotate the knurled locking nut upward to lock the safety screw in place.

6. Now, turn the focus knob (3) until the specimen comes into sharp focus.

Warning: Never

4

Laboratory Microscope – Model # 44102

10. Arm

11. Fine Focus Knob

9. Safety Rack Stop

8. Spring Loaded
Stage Clips

Figure 2

1. Eyepiece

2. Eyepiece Tube

3. Coarse Focus Knob

12. Nosepiece

4. Objective Lens

5. Specimen Stage

6. Illumination Mirror

7. Base

Standard Accessories with your Microscope

• 10x Huygens Eyepiece • 40x Objective Lens

• 4x Objective Lens • 5 Prepared Slides

• 10x Objective Lens • Dust Cover

Specifications

Model # 44102 Specifications
Stage Plain Stage with spring loaded clips -- 120mm x 110mm
Arm Angle choice from 0° to 60°
Focuser Coarse and Fine focus knobs; Rack and Pinion Safety Stop
Objectives Achromatic -- 4x ( 40 power ), 10x ( 100 power), 40x ( 400 power)
Illuminator 50mm Plano-Concave Mirror
Eyepiece 10x -- 12mm Field of View
Nosepiece Triple with click stop
Condenser N.A. 0.65
Diaphragm Disc Diaphragm with five aperture sizes

5

Setting Up Your Microscope

1. Take the Styrofoam container out of the cardboard carton.

2. Remove the tape from the Styrofoam container holding the two sections together.

3. Carefully remove the microscope and other parts from the container and set them on a table, desk, or other flat

surface.

4. Remove the plastic bag covering the microscope.

5. Remove the protective paper from the stage (5).

6. Remove the plastic cap from the eyepiece tube (2).

7. Insert the 10x eyepiece in the eyepiece tube (2).

8. Remove the three objective lenses (4) from their containers. Unscrew the container lids from the threaded portion

of the objective lenses.

9. Thread the end of the 4mm objective lens into one of the holes on the nosepiece (12) until finger tight. It may be

necessary to lower the stage (5) by turning the coarse focus knob (3).

10. Now turn the nosepiece to the next opening and thread each of the rem aining objective lenses into the remaining

holes.

You are now ready to use your microscope!

Microscope Operation

Using the Illuminator Mirror

Your microscope has a built-in plano-concave illumination mirror which allows you to illuminate the specimen from the
bottom by reflecting an external light source (indirect sunlight, room light, lamp, etc.) to the stage. The mirror has a flat
side (you can easily see your reflection on the flat surface) and a concave side. The concave side will concentrate more
light onto the specimen than the flat side. To illuminate a specimen:

1. If it is daylight, tilt the mirror (6) and reflect indirect sunlight through the bottom of the stage (5).

use the mirror to focus direct sunlight through the microscope as this can be very harmful and may damage your
eyes.

2. If viewing at night or indoors, away from sunlight, you can use a nearby desk lamp, table lamp, or other light source

to reflect light onto a specimen.

To control the amount of light reflecting onto the specimen:

1. Use the concave side of the mirror to focus more light on the slide. The concave side will concentrate more light onto
your specimen but will focus a smaller beam of light, making it more sensitive to adjust.

2 If you are unable to adjust the mirror enough to properly illuminate the specimen, you can also tilt the arm (10)

backward to change the position of the mirror.

3. The amount of light can also be controlled by rotating the five-aperture size disc diaphragm (see figure 2a) underneath
the specimen stage (5).

Adjusting the Arm Angle

The normal viewing position is at 0°. However, you can view at any angle from 0° to 60°. To change the viewing angle:

1. With one hand hold the base (7).

2. Then, with your other hand, tilt the arm (10) by pulling it backward or forward to the desired viewing angle.

6

Warning: Never

Condenser

Disc Diaphragmc

Diaphragm

Figure 2a

Viewing a Specimen

Carefully place a specimen slide under the spring loaded stage clips (8) and center the specimen directly over the hole in the
center of the stage (5).

You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the
specimen slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the
range of travel on the focuser. This assures that the objective lens will not accidentally come into contact with the
specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focus mechanism:

1. Raise the objective lens away from the specimen stage (5) by rotating the coarse focus knob (3) backwards

(clockwise).

2. Unthread the safety rack stop screw (9) about 2/3 of the way.

3. Turn the nosepiece (12) until the 40x lens is over the specimen.

4. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to

touch the objective lens to the slide).

5. Thread the safety screw (9) upward until it stops against the focus mechanism.

6. Rotate the knurled locking nut upward to lock the safety screw in place.

Focusing

Now that the specimen slide is placed directly under the objective lens, use the coarse focus knob (3) to focus on the
specimen.

1. Always start with the lowest power (4x objective lens) and switch to higher power by rotating the nosepiece (12) to

the 10x and then 40x objective lenses. Be cautious not to let the objective lens touch the specimen slide when
changing to higher power.

2. Use the fine focus knob (11) to change the depth of field and obtain precise focus on the specimen you are observing.

3. If you run out of downward travel before reaching sharp focus, you can unscrew the safety screw (9) to allow for

more downward travel. Once again, be careful not to let the objective lens touch the specimen slide.

7

Model # 44104 Specifications
Stage Mechanical Stage 115mm x 125mm
Arm Angle choice from 0° to 60°
Focuser Coarse and Fine focus knobs; Rack & Pinion Safety Stop
Objectives Achromatic -- 4x, 10x, 40x
Illuminator 50mm Plano-Concave Mirror
Eyepieces 10mm -- 12mm Field of View; 12.5mm -- 10mm Field of View
Nosepiece Triple with click stop
Condenser Abbe N.A. 1.25
Diaphragm Iris

Advanced Microscope – Model # 44104

1. Eyepiece

2. Eyepiece Tube

3. Coarse Focus Knob

10. Arm

12. Nosepiece

11. Fine Focus Knob

9. Safety Rack Stop

8. Stage Holder Clamp

6. Illumination Mirror

7. Base

Figure 3

Standard Accessories with your Microscope

• 10x Huygens Eyepiece • Electric Illuminator

• 12.5 Huygens Eyepiece • Blue Filter

• 4x Objective Lens • 5 Prepared Slides

• 10x Objective Lens • Dust Cover

• 40x Objective Lens

Specifications

4. Objective Lens

5. Specimen Stage

8

Magnification Table

Use the following table to determine the magnification of the different eyepiece/objective lens combination of your
microscope.

Objective Lens 4x 10x 40x
10x Eyepiece

12.5x Eyepiece

40x 100x 400x
50x 125x 500x

Setting Up Your Microscope

1. Take the Styrofoam container out of the cardboard carton.

2. Remove the tape from the Styrofoam container holding the two sections together.

3. Carefully remove the microscope and other parts from the container and set them on a table, desk, or other flat

surface.

4. Remove the plastic bag covering the microscope.

5. Remove the protective paper from the stage (5).

6. Remove the plastic cap from the eyepiece tube (2).

7. Insert the 10x eyepiece in the eyepiece tube (2).

8. Remove the three objective lenses (4) from their containers. Unscrew the container lids from the threaded portion

of the objective lenses.

9. Thread the end of the 4mm objective lens into one of the holes on the nosepiece (12) until finger tight. It may be

necessary to lower the stage (5) by turning the coarse focus knob (3).

10. Now turn the nosepiece to the next opening and thread each of the remaining objective lenses into the remaining
holes. You are now ready to use your microscope!

Microscope Operation

Using the Illuminator Mirror

Your microscope has a built-in plano-concave illumination mirror which allows you to illuminate the specimen from the
bottom by reflecting an external light source (indirect sunlight, room light, lamp, etc.) to the stage. The mirror has a flat
side (you can easily see your reflection on the flat surface) and a concave side. The concave side will concentrate more
light onto the specimen than the flat side. To illuminate a specimen:

1. If it is daylight, tilt the mirror (6) and reflect indirect sunlight through the bottom of the stage (5).

use the mirror to focus direct sunlight through the microscope as this can be very harmful and may damage your
eyes.

2. If viewing at night or indoors, away from sunlight, you can use a nearby desk lamp, table lamp, or other light source

to reflect light onto a specimen.

To control the amount of light reflecting onto the specimen:

1. Use the concave side of the mirror to focus more light on the slide. The concave side will concentrate more light onto
your specimen but will focus a smaller beam of light, making it more sensitive to adjust.

2 If you are unable to adjust the mirror enough to properly illuminate the specimen, you can also tilt the arm (10)

backward to change the position of the mirror – see the section under “Adjusting the Arm Angle”.

3. You can also control light adjustment with the condenser an d diaphragm -- see the section under “Adjusting the

Lighting”.

Warning: Never

9

Adjusting the Arm Angle

The normal viewing position is at 0°. However, you can view at any angle from 0° to 60°. To change the viewing angle:

1. With one hand hold the base (7).

2. Then, with your other hand, tilt the arm (10) by pulling it backward or forward to the desired viewing angle.

Using the Electric Illuminator

Figure 3a Figure 3b Figure 3c Figure 3d

For more direct and intensive light, you should use the supplied electric illuminator. To install the electric illuminator:

1. Remove the mirror illuminator by pulling it outward from its sleeve. It will come out very easily with little pressure –

see Figure 3a.

2. You will install the electric illuminator in the sleeve you removed the mirror illuminator from – see Figure 3a.

3. Be sure the glass part of the electric illuminator (see Figure 3b) is facing up towards the stage (5).

4. Place the illuminator pin and clips over the sleeve and push until it is in all the way – see Figure 3c.

5. Plug in the cord of the electric illuminator to the proper AC power source – see Figure 3d.

Changing Eyepieces

Your microscope is supplied with both 10x and 12.5x eyepieces. They are easily interchanged:

1. Remove either eyepiece (1) from the eyepiece tube (2) by pulling it upward.

2. Install the desired eyepiece (1) into the eyepiece tube (2) by pushing downward.

3. The eyepieces are held in place by a friction fit.

Viewing a Specimen

Your instrument is provided with a mechanical stage with a stage holder clamp and directional knobs – see figure 3e.

1. Use the clamp lever to open the clamping arm of the stage holder clamp.

2. Place a specimen slide (3” size) inside the holder and gently close the clamping arm against the slide.

3. Use the stage movement knobs (see Figure 3e) to position the specimen over the opening in the stage (5).

You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the
specimen slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the
range of travel on the focuser. This assures that the objective lens will not accidentally come into contact with the
specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focus mechanism:

4. Raise the objective lens away from the specimen stage (5) by rotating the coarse focus knob (3) backwards

(clockwise).

5. Unthread the safety rack stop screw (9) about 2/3 of the way.

6. Turn the nosepiece (12) until the 40x lens is over the specimen.

7. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to

touch the objective lens to the slide).

8. Thread the safety screw (9) upward until it stops against the focus mechanism.

9. Rotate the knurled locking nut upward to lock the safety screw in place.

10

Stage Holder

Note: The top stage movement knob moves in the X

axis (forward and backward) whereas the bottom stage

movement knob moves in the Y axis (side to side). A

vernier scale on both axes allows the exact marking and replication of an

object in the field of view that the user may want to

Stage Movement
Knobs

come back to.

Figure 3e

Tip: To position the specimen directly under the objective lens, close the opening on the iris diaphragm (see Figure 3f)

until it is almost completely closed. You should see a small beam of light projected onto the specimen slide. Now simply
use the stage movement knobs to move the specimen directly inside the beam of light.

10. Use the nosepiece (12) to rotate the objective lenses (4) until the 4x objective is directly over the specimen. Always

start with the lowest power objective and work your way up to higher powers.

11. Look through the eyepiece while turning the coarse focus knob (3) until the specimen comes into view. You may

need to adjust the stage knobs (Figure 3e) slightly to center the specimen in the field of view. Warning: When

focusing, be careful not to raise the specimen stage so high that the specimen slide touches the objective lens.
Not only can you break your slide but you may scratch the objective lens.

12. Finally, adjust the fine focus knob (11) until you reach the sharpest focus for your eye. Warning: Never rotate

both of the fine focus knobs at the same time in opposite directions or the focusing mechanism may get
damaged.

Abbe Condenser

Condenser Regulator Knob

Iris Diaphragm Lever

Figure 3f

11

Adjusting the Lighting

Specimens of different size, thickness, and color variations will require different levels of illumination. There are two ways
to change the amount of illumination when viewing a specimen; adjusting the Abbe condenser and adjusting the iris
diaphragm (see Figure 3f):

1. When viewing with lower power (4x and 10x) objective lenses you will need to lower the condenser lens in order to
spread the light over the larger field of view. To change the position of the condenser, simply rotate the condenser
regulator knob clockwise until the beam of light spreads wide enough to illuminate the entire field of view when
viewing.

2. As you lower the condenser to spread out the light or change to a higher power objective lens, your image will appear

dimmer. Open the aperture of the iris diaphragm to let in more light. Opening and closing the diaphragm will give
a relief view of the specimen and allow you to change the depth of field of the specimen being viewed.

Using a Filter

To bring out different levels of detail, experiment with changing the color of the back lighting of the specimen. To change
the lighting color, place the blue filter in the light path by placing it on the top of the electric illuminator. You may need to
refocus by adjusting the fine focus knob (11) slightly for best viewing.

Replacing the Electric Illuminator Bulb

Thumbscrew

To replace a burned out bulb, do the following:

1. Take out the thumbscrew on the

illuminator by turning it counter
clockwise.

2. Open the compartment and you will s

the 20 watt tungsten bulb.

3. Remove the bulb by pressing lightly

down on the bulb and rotate it counter
clockwise and it will come out.

4. Take the new bulb and line up the pins

of the bulb with the slots in the bulb
socket and then push it down and rotate
clockwise until it sets in place.

5. Close the compartment, line up the

.threaded hole for the thumbscrew and

Figure 3g tighten it by turning clockwise.

ee

12

9. Safety Rack Stop

8. Stage Holder Clamp

3. Coarse Focus Knob

11. Fine Focus Knob

1. Eyepiece

2. Eyepiece Tube

10. Arm

Advanced Microscope -- Model # 44106

14. Head

12. Nosepiece

5. Specimen Stage

6. LED Illuminator

13. On/Off Switch

Figure 4

Standard Accessories with your Microscope

4. Objective Lens

7. Base

• 10x Eyepiece with pointer • LED Illuminator

• 4x Objective Lens • Green, Yellow, Blue Filters

• 10x Objective Lens • Immersion Oil

• 40x Objective Lens • 5 Prepared Slides

• 100x Objective Lens • Dust Cover

Specifications

Model #44106 Specifications
Stage Mechanical Stage 115mm x 125mm
Head Monocular with 45° incline and 360° rotatable
Focuser Coarse and Fine focus knobs; Rack & Pinion Safety Stop
Objectives Achromatic- 4x (40 power), 10x (100 power),
40x (400 power), 100x (1000 power)
Illuminator 50mm Plano-Concave Mirror
Eyepiece 10x Wide Field with Pointer -- 18mm Field of View
Nosepiece Quadruple with click stop
Condenser Abbe N.A. 1.25
Diaphragm Iris

13

Setting Up Your Microscope

1. Take the Styrofoam container out of the cardboard carton.

2. Remove the tape from the Styrofoam container holding the two sections together.

3. Carefully remove the microscope and other parts from the container and set them on a table, desk, or other flat

surface.

4. Remove the plastic bag covering the microscope.

5. Remove the protective paper from the stage (5).

6. Remove the four objective lenses (4) from their containers. Unscrew the container lids from the threaded portion

of the objective lenses.

7. Thread the end of the 4mm objective lens into one of the holes on the nosepiece (12) until finger tight. It may be

necessary to lower the stage (5) by turning the coarse focus knob (3).

8. Now turn the nosepiece to the next opening and thread each of the remaining objective lenses into the remaining
holes.

You are now ready to use your microscope!

Microscope Operation

Using the Illuminator Mirror

Your microscope has a built-in plano-concave illumination mirror which allows you to illuminate the specimen from the
bottom by reflecting an external light source (indirect sunlight, room light, lamp, etc.) to the stage. The mirror has a flat
side (you can easily see your reflection on the flat surface) and a concave side. The concave side will concentrate more
light onto the specimen than the flat side. To illuminate a specimen:

1. If it is daylight, tilt the mirror (6) and reflect indirect sunlight through the bottom of the stage (5).

use the mirror to focus direct sunlight through the microscope as this can be very harmful and may damage your
eyes.

2. If viewing at night or indoors, away from sunlight, you can use a nearby desk lamp, table lamp, or other light source

to reflect light onto a specimen.

To control the amount of light reflecting onto the specimen:

1. Use the concave side of the mirror to focus more light on the slide. The concave side will concentrate more light onto
your specimen but will focus a smaller beam of light, making it more sensitive to adjust.

2. You can also control light adjustment with the condenser and diaphragm -- see the section under “Adjusting the
Lighting”.

Rotating the Head

The head of your microscope can be rotated 360°. Therefore, you can view from any position by just moving the head to
the desired location.

14

Warning: Never

Using the LED Illuminator

For more direct and intensive light, you should use the supplied LED illuminator. The LED illuminator is powered by three
AA batteries (user supplied).

1. Remove the mirror illuminator by pulling it outward from its sleeve.

2. The LED illuminator tube will thread counterclockwise on over the LED’s in the base of the microscope – see Figure

4a. Turn the tube until finger tight – see Figure 4b.

3. Install the batteries – see the next section below.

4. After the batteries are installed, turn the power switch (13) to the “on” position.

5. Make sure that on the back of the microscope base that the switch is in the “Btry” position – see Figure 4c.

Figure 4a Figure 4b Figure 4c

Installing Batteries for the LED Illuminator

Figure 4d Figure 4e Figure 4f

To install the three AA batteries:

1. Turn the microscope on its side.

2. With a small Phillips head screwdriver, remove the four screws/rubber feet at the bottom of the base and the screen

can then be removed – see Figure 4d.

3. Remove the two shiny Phillips head screws holding a bracket which keeps the battery box in place – see Figure 4e.

4. Slide open the battery box.

5. Install three AA batteries following the “+” and “-“indicators – see Figure 4f.

6. Close the battery box.

7. Replace the bracket holding the battery box in place with the two shiny Phillips head screws.

8. Replace the screen with the four Phillips head screws/rubber feet.

15

Viewing a Specimen

Your instrument is provided with a mechanical stage with a stage holder clamp and directional knobs –see figure 4g and 4h.
A vernier scale on both axes allows the exact marking and replication of an object in the field of view that the user may
want to come back to.

1. Use the clamp lever to open the clamping arm of the stage holder clamp.

2. Place a specimen slide (3” size) inside the holder and gently close the clamping arm against the slide.

3. Use the stage movement knobs (see Figure 4h) to position the specimen over the opening in the stage (5).

You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the
specimen slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the
range of travel on the focuser. This assures that the objective lens will not accidentally come into contact with the
specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focus mechanism:

4. Raise the objective lens away from the specimen stage (5) by rotating the coarse focus knob (3) backwards

(clockwise).

5. Unthread the safety rack stop screw (9) about 2/3 of the way.

6. Turn the nosepiece (12) until the 40x lens is over the specimen.

7. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to

touch the objective lens to the slide).

8. Thread the safety screw (9) upward until it stops against the focus mechanism.

9. Rotate the knurled locking nut upward to lock the safety screw in place.

Figure 4g Figure 4h

Tip: To position the specimen directly under the objective lens, close the opening on the iris diaphragm (see Figure 4h)

until it is almost completely closed. You should see a small beam of light projected onto the specimen slide. Now simply
use the stage movement knobs to move the specimen directly inside the beam of light.

10. Use the nosepiece (12) to rotate the objective lenses (4) until the 4x objective is directly over the specimen. Always

start with the lowest power objective and work your way up to higher powers.

11. Look through the eyepiece while turning the coarse focus knob (3) until the specimen comes into view. You may

need to adjust the stage knobs (Figure 4h) slightly to center the specimen in the field of view. Warning: When

focusing, be careful not to raise the specimen stage so high that the specimen slide touches the objective lens.
Not only can you break your slide but you may scratch the objective lens.

12. Finally, adjust the fine focus knob (11) until you reach the sharpest focus for your eye. Warning: Never rotate

both of the fine focus knobs at the same time in opposite directions or the focusing mechanism may get
damaged.

Tip: When viewing a specimen with the 100x objective lens, you can improve the resolving power by placing a small drop

of immersion oil between the specimen and objective lens. For specimen slides that you prepare yourself, always cover the
specimen with a thin piece of glass and place the oil on the cover glass. Do not put the oil directly on the specimen sample.

Abbe Condenser

Iris Diaphragm

Stage
Movement Knobs

16

Adjusting the Lighting

Specimens of different size, thickness, and color variations will require different levels of illumination. There are three
ways to change the amount of illumination when viewing a specimen; adjusting the brightness control on the on/off knob
(13), adjusting the Abbe condenser (Figure 4g) and adjusting the iris diaphragm (see Figure 4h):

1. When viewing a specimen that is not transparent or dark in color you may need to increase the amount of light to

resolve certain features or details. This is best done by simply increasing the brightness of the LED illuminator by
rotating the brightness control on the on/off switch (13) all the way to its highest setting.

2. When viewing with lower power (4x and 10x) objective lenses you will need to lower the condenser lens in order to
spread the light over the larger field of view. To change the position of the condenser, simply rotate the silver center
portion of the Iris diaphragm (4h) clockwise until the beam of light spreads wide enough to illuminate the entire field
of view when viewing.

3. As you lower the condenser to spread out the light or change to a higher power objective lens, your imag e will appear

dimmer. Instead of increasing the light intensity of the illuminator (which may “wash out” fine detail of the specimen
you are viewing), open the aperture of the iris diaphragm to let in more light. Opening and closing the diaphragm
will give a relief view of the specimen and allow you to change the depth of field of the specimen being viewed.

Using Filters

To bring out different levels of detail, experiment with changing the color of the back lighting of the specimen. To change
the lighting color, place the blue filter, the green filter, or the yellow filter in the light path by placing one on top of the
LED illuminator. You may need to refocus by adjusting the fine focus knob (11) slightly for best viewing. You should
experiment with each of the colors to see the results.

17

Care and Maintenance

Your Celestron microscope is a precision optical instrument and should be treated with care at all times. Follow these care
and maintenance suggestions and your microscope will need very little maintenance throughout its lifetime.

• When you are done using your microscope, remove any specimens left on the stage.

• Turn off any electric illuminator knobs or switches.

• Unplug any power cords being used.

• Always place the dust cover over the microscope when not in use or when being stored.

• Store the microscope in a dry and clean place.

• Be very careful if using your microscope in direct sun light to prevent damage to the microscope or your eyes.

• When moving your telescope, carry it by the “arm” with one hand and not by the focuser knobs, eyepiece housing,

etc. Then, put your other hand under the base for support.

• Clean the outside surfaces (metal and plastics) with a moist cloth.

• Always unplug any cords before cleaning.

• Never clean optical surfaces with cloth or paper towels as they can scratch optical surfaces easily.

• Blow off dust with a camels hair brush or an air blower from optical surfaces.

• To clean fingerprints off of optical surfaces, use a lens cleaning agent and lens tissue available at most photo

outlets and when cleaning do not rub in circles as this may cause sleeks or scratches to occur.

• Never disassemble or clean internal optical surfaces. This should be done by qualified technicians at the factory or

other authorized repair facilities.

• When handling glass specimen slides, be care as the edges can be sharp.

18

CCeelleessttrroonn TTwwoo YYeeaarr WWaarrrraannttyy

A. Celestron warrants this microscope to be free from defects in materials and workmanship for two years. Celestron will repair or replace such

product or part thereof which, upon inspection by Celestron, is found to be defective in materials or workmanship. As a condition to the obligation of
Celestron to repair or replace such product, the product must be returned to Celestron together with proof-of-purchase satisfactory to Celestron.

B. The Proper Return Authorization Number must be obtained from Celestron in advance of return. Call Celestron at [phone] to receive the

number to be displayed on the outside of your shipping container.

All returns must be accompanied by a written statement setting forth the name, address, and daytime telephone number of the owner, together with a
brief description of any claimed defects. Parts or product for which replacement is made shall become the property of Celestron.

The customer shall be responsible for all costs of transportation and insurance, both to and from the factory of Celestron, and shall be
required to prepay such costs.

Celestron shall use reasonable efforts to repair or replace any microscope covered by this warranty within thirty days of receipt. In the event repair
or replacement shall require more than thirty days, Celestron shall notify the customer accordingly. Celestron reserves the right to replace any
product which has been discontinued from its product line with a new product of comparable value and function.

This warranty shall be void and of no force of effect in the event a covered product has been modified in design or function, o r subjected to
abuse, misuse, mishandling or unauthorized repair. Further, product malfunction or deterioration due to normal wear is not covered by
this warranty.

CELESTRON DISCLAIMS ANY WARRANTIES, EXPRESS OR IMPLIED, WHETHER OF MERCHANTABILITY OF FITNESS FOR A
PARTICULAR USE, EXCEPT AS EXPRESSLY SET FORTH HEREIN.

THE SOLE OBLIGATION OF CELESTRON UNDER THIS LIMITED WARRANTY SHALL BE TO REP AIR OR REPLACE THE COVERED
PRODUCT, IN ACCORDANCE WITH THE TERMS SET FORTH HEREIN. C ELESTRON EXPRESSL Y DISCLAIMS ANY LOST PROF ITS,
GENERAL, SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGE S WHICH MAY RESULT FROM BREACH OF ANY WARRANTY, OR
ARISING OUT OF THE USE OR INABILITY TO USE ANY CELESTRON PRODUCT. ANY WARRANTIES WHICH ARE IMPLIED AND
WHICH CANNOT BE DISCLAIMED SHALL BE LIMITED IN DURATION TO A TERM OF TWO YEARS FROM THE DATE OF
ORIGINAL RETAIL PURCHASE.

Some states do not allow the exclusion or limitation of incidental or consequential damages or limitation on how long an implied warranty lasts, so
the above limitations and exclusions may not apply to you.

This warranty gives you specific legal rights, and you may also have other rights which vary from state to state.

Celestron reserves the right to modify or discontinue, without prior notice to you, any model or style microscope.

If warranty problems arise, or if you need assistance in using your microscope contact:

Celestron
Technical Support Department
2835 Columbia Street
Torrance, CA 90503 U.S.A.

Tel. [phone]
Fax. [phone]
www.celestron.com
Monday-Friday 8AM-4PM PST

This warranty supersedes all other product warranties.

NOTE: This warranty is valid to U.S.A. and Canadian customers who have purchased this product from an Authorized Celestron Dealer in
the U.S.A. or Canada. Warranty outside the U.S.A. and Canada is valid only to customers who purchased from a Celestron Distributor or
Authorized Celestron Dealer in the specific country and please contact them for any warranty service.

19

MMAANNUUAALL DDEE IINNSSTTRRUUCCCCIIOONNEESS

●● MMooddeelloo ddee llaabboorraattoorriioo NNºº 4444110000 ●● MMooddeelloo ddee

v

●● MMooddeelloo aav

aannzzaaddoo NNºº 4444110044 ●● MMooddeelloo aavvaannzzaaddoo NNºº 4444110066

1

llaabboorraattoorriioo NNºº 4444110022

Índice

Introducción …………………………………………………………………………...2

Modelo de laboratorio Nº 44100 ……………………………………………………...3

Modelo de laboratorio Nº 44102 ……………………………………………………...5

Modelo avanzado Nº 44104 ……………………………………………………………8

Modelo avanzado Nº 44106 …………………………………………………………..13

Cuidado y mantenimiento …………………………………………………………....18

Garantía ……………………………………………………………………………….19

Introducción

Felicitaciones por haber adquirido un microscopio Celestron. Su microscopio es un instrumento óptico de precisión,
fabricado con materiales de la más alta calidad para asegurarle durabilidad y una larga vida útil. Está diseñado para que
pueda disfrutarlo al máximo con un mínimo de mantenimiento.

Este manual de instrucciones cubre cuatro modelos de microscopios. Encuentre su modelo específico para cerciorarse de
que lee la información correcta.

Antes de usar el microscopio, lea detenidamente las instrucciones para familiarizarse con las funciones y funcionamiento
para que pueda disfrutarlo y utilizarlo a su máxima potencia. Vea los diagramas del microscopio para ubicar las partes
que se describen en este manual.

Los microscopios que se describen en este manual proporcionan aumentos de 40 hasta 1000x. Estos tipos de
microscopios son ideales para el estudio de portaobjetos de levaduras y mohos, cultivos, partes de plantas o animales,
fibras, bacterias, etc.

La sección final contiene consejos para el fácil cuidado y mantenimiento del microscopio que deberá seguir para
aprovechar años de rendimiento, uso y aplicación de calidad.

2

9. Tope de seguridad
de la platina

8. Presillas a resorte
de la platina

Microscopio de laboratorio – Modelo Nº 44100

10. Brazo

3. Perilla de enfoque

1. Ocular

2. Tubo del ocular

4. Objetivo

5. Platina

Figura 1

6. Espejo luminoso

7. Base

Accesorios estándar incluidos con el microscopio

• Ocular Huygens de 10x • 5 preparaciones en portaobjetos

• Objetivo de 20x (200x con ocular) • Cubierta contra el polvo

Especificaciones

Modelo Nº 44100 Especificaciones
Platina Platina lisa con presillas a resorte – 88 mm x 88 mm
Brazo Selección de ángulo de 0° a 45°
Mecanismo de enfoque Macrométrico; mecanismo con piñón y cremallera con tope de seguridad
Objetivo Acromático 20x
Iluminador Espejo plano-cóncavo de 42 mm

Ocular 10x – Campo visual de 12 mm

3

1. Retire el contenedor de espuma de poliestireno de la caja de cartón.

2. Quite la cinta del contenedor de poliestireno que sujeta las dos secciones.

3. Retire cuidadosamente el microscopio y otras piezas y colóquelos sobre una mesa, escritorio o cualquier otra

superficie plana.

4. Retire la bolsa de plástico que cubre el microscopio.

5. Quite el papel protector de la platina (5).

6. Retire la tapa de plástico del tubo del ocular (2).

7. Inserte el ocular de 10x en el tubo del ocular (2). ¡Ahora puede usar su microscopio!

Manejo del microscopio

Uso del espejo luminoso

El microscopio cuenta con un espejo luminoso plano-cóncavo incorporado que permite iluminar el espécimen desde
abajo reflejando en la platina una fuente de luz externa (luz solar indirecta, luz de la habitación, lámpara, etc.). El espejo
tiene un lado plano (usted puede ver su reflejo en la superficie plana) y un lado cóncavo. El lado cóncavo concentra
mucha más luz sobre el espécimen que el lado plano. Para iluminar el espécimen:

1. Si tiene luz natural, incline el espejo (6) y refleje la luz indirecta desde la parte de abajo de la platina (5).

Advertencia: Nunca utilice el espejo para concentrar la luz solar directa a través del microscopio ya que puede
ser muy dañina y dañar la vista.

2. Si se utiliza de noche o adentro, sin luz solar, puede usar una lámpara de escritorio cercana, una lámpara de mesa

u otra fuente luminosa para reflejar la luz sobre el espécimen.

Para controlar la cantidad de luz que se refleja en el espécimen:

1. Use el lado cóncavo del espejo para concentrar más luz en el portaobjetos. El lado cóncavo concentrará más luz en
el espécimen pero concentrará un haz mucho más pequeño, haciéndolo más sensible para regular.

2 Si no puede regular el espejo para que ilumine el espécimen correctamente, puede cambiar la posición del espejo

inclinando el brazo (10) hacia atrás.

Ajuste del ángulo del brazo

La posición normal de observación es 0º. Sin embargo, también se puede hacer desde cualquier ángulo de 0º a 45º. Para
cambiar el ángulo de observación:

1. Sostenga la base con una mano (7).

2. Después, con la otra mano, incline el brazo (10) moviéndolo hacia adelante o hacia atrás hasta el ángulo de
observación deseado.

Observación del espécimen

Cuidadosamente coloque un portaobjetos debajo de las presillas a resorte de la platina (8) y centre el espécimen
directamente sobre el agujero en el centro de la platina (5).

Ahora está listo para enfocar y observar el espécimen, pero primero debe tomar ciertas precauciones para no dañar el
portaobjetos o el objetivo. El microscopio está equipad o co n un to pe de segu ri dad de la platina (9) que permite regular la
distancia de recorrido del mecanismo de enfoque. Esto asegura que el objetivo no haga contacto accidentalmente con el
portaobjetos ya que podría romperlo o podría rayarse el objetivo. Para regular la distancia de recorrido del mecanismo
de enfoque:

1. Haga girar la perilla de enfoque (3) hacia atrás (en el sentido de las agujas del reloj) para elevar el objetivo

alejándolo de la platina (5).

2. Desenrosque unos 2/3 del tornillo del tope de seguridad de la platina (9).

3. Baje el objetivo sobre el portaobjetos hasta que la punta del lente esté casi encima del mismo. (Tenga mucho

cuidado y no permita que el objetivo toque el portaobjetos).

4. Enrosque el tornillo de seguridad (9) hacia arriba hasta que se tope contra el mecanismo de enfoque.

5. Haga girar la tuerca de retención estriada hacia arriba para trabar el tornillo de seguridad.

6. Ahora, gire la perilla de enfoque (3) hasta que el espécimen esté bien enfocado.

Desembalaje y armado del microscopio

4

10. Brazo

11. Perilla micrométrica

9. Tope de seguridad
de la platina

8. Presillas a resorte
de la platina

• Ocular Huygens de 10x • Objetivo de 40x

• Objetivo de 4x • 5 preparaciones en portaobjetos

• Objetivo de 10x • Cubierta contra el polvo

Microscopio de laboratorio – Modelo Nº 44102

1. Ocular

2. Tubo del ocular

3. Perilla macrométrica

12. Revólver

4. Objetivo

5. Platina

6. Espejo luminoso

7. Base

Figura 2

Accesorios estándar incluidos con el microscopio

Especificaciones

Modelo Nº 44102 Especificaciones
Platina Platina lisa con presillas a resorte – 120 mm x 110 mm
Brazo Selección de ángulo de 0° a 60°
Mecanismo de enfoque Perillas macrométrica y micrométrica; tope de seguridad de piñón y cremallera
Objetivos Acromático – 4x (aumento de 40), 10x (aumento de 100), 40x (aumento de 400)
Iluminador Espejo plano-cóncavo de 50 mm
Ocular 10x – Campo visual de 12 mm
Revólver Triple con clic de parada
Condensador N.A. 0,65
Diafragma Diafragma de disco con cinco tamaños de apertura

5