Motic BA410 Instruction Manual

We are constantly endeavouring to improve our instruments and to adapt them to the
requirements of modern research techniques and testing methods. This involves
modification to the mechanical structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all
specifications are subject to change without notice.

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INFINITY CORRECTED OPTICS

In this optical concept the light beams are parallel after leaving the objective in the
direction of the eyepieces. A second optical element, the tube lens (normally located in
the eyepiece tube) is used to converge the parallel beams, resulting in an intermediate
image. The intermediate image is focussed by the eyepieces, to provide the real image
for visual observation.
The implementation of a tube lens gives the opportunity to minimize chromatic
aberrations and other “optical defects”. Further, in “Infinity Optics” the distance between
the objective and tube lens is not as strictly fixed as in the (historically older) “Finite
Optics” of 160mm tube length.
This allows additional optical components to be inserted between the objective and tube
head.
Fluorescence attachments, discussion bridges, eye level risers and other options can be
added without affecting the image quality.
In general “Infinity Optics” provides flexibility and the opportunity to add additional
optional features.

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TABLE OF CONTENTS

Section
I Nomenclature 4
II Setting up the instrument 7

Operating environment 7

III Assembling the microscope 7

Verifying Input voltage 7
1 Mechanical stage 8
2 Specimen holder 8
3 Objectives 8
4 Condenser 9
5 Eyepiece tube 9
6 Eyepieces 10
7 Illumination 10
8 Filters 11
9 Power cord 12

IV Microscopy 12

1 Coarse and fine focusing 12
2 Coarse focus torque adjustment 12
3 Coarse focus quick stop 13
4 Stage upper limit stop adjustment 13
5 Beam splitter lever 14
6 Interpupillary distance adjustment 15
7 Diopter adjustment 15
8 Centering the condenser 16
9 Use of aperture diaphragm 17
10 Use of field diaphragm 18
11 Brightness and contrast adjustment 18

V Photomicrographic procedure 19
VI Using Oil Immersion Objectives 20
VII Troubleshooting Table 21
VIII Care and maintenance 23
IX Warning label 26

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I. Nomenclature

MOTIC BA410 (30W Halogen)

4

MOTIC BA410 (Trinocular) 30W Halogen

5

MOTIC BA410 30W Halogen

6

II. Setting up the instrument

Avoid placing the instrument in locations exposed to direct sunlight, dust, vibration, high
temperature, high humidity and where it is difficult to unplug the power supply cord.

Operating environment

y Indoor use
y Altitude: Max 2000 meters
y Ambient temperature: 15°C to 35°C

y

Maximum relative humidity: 75% for temperature up to 31ºC decreasing linearly to

50% relative humidity at 40ºC

y

Supply voltage fluctuations: Not to exceed ±10% of the normal voltage

y

Pollution degree: 2 (in according with IEC60664)

y Installation / Overvoltage category: 2 (in according with IEC60664)

y

Air Pressure of 75kPa to 106kPa

y Avoid frost, dew, percolating water, and rain

III. Assembling the microscope

Verifying Input voltage

y The automatic voltage selection works with a broad range of settings. However,
always use a power cord that is rated for the voltage used in your area and that has
been approved to meet local safety standards. Using the wrong power cord could
cause fire or equipment damage.
y If using an extension cord, use only a power supply cord with a protective earth (PE)
wire.
y In order to prevent electric shock, always turn the power supply off before connecting
the power cord.

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y Electrical Specifications:

30W Halogen Bulb

Input: 90-240V~, 80VA, 50-60Hz
Bulb: 6V 30W Halogen
Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

1. Mechanical stage

y Loosen the stage clamp screw; fit the stage into the circular dovetail mount. An
orientation notch at the back of the stage has to fit to an orientation pin at the stage
carrier. Tighten with the clamp screw facing forward.

y Torque adjustment

The torque of the y-axis and x-axis travel knobs can be adjusted by torque
adjusting knobs.
For y-axis to (+) increase or (-) decrease the torque, turn Knob Y while holding A
For x-axis to (+) increase or (-) decrease the torque, turn Knob X while holding B

2. Specimen holder

y Attach the specimen holder, using the two mounting holes. If the stage is to be used
with right right-hand controls, attach the specimen holder to the left-hand holes and
similarly, with left-hand controls to the right-hand holes.

3. Objectives

y Lower the stage completely. Screw the objectives into the revolving nosepiece so
that clockwise rotation of the nosepiece brings the next higher magnification objective
into position.

8