Motic B1-220A Instruction Manual

Instruction Manual

B1 Series

Copyright © 02/02
Motic Microscopes, European Division

1. Eyepieces 13. Sliding
interpupilliar

y

adjustment.

2. Interpupillary

Scale

3. Revolving

Nosepiece

4.Objectives

5. Moveable finger

6. Specimen holder

mechanism

7. Slide holder

8. Stage

10. Condenser

9. Diaphragm

15. Head

17. Arm

14. Eyepiece tube with
diopter correctors

18. Knurled locking

screws for securing

specimen holder

19. Coarse Focus
Knob

20. Fine Focus
Knob

21. Knobs
controlling

16. Knurled locking
screw

stage movemen

t

11. Filter holder

22. Illuminato

r

12. Base

23. Ligh

t

intensity control

B1-220A

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Introduction

Thank you for your purchase of a Motic microscope. Motic microscopes are precision instruments, subjected
to meticulous examination in order to reach you in perfect condition. Their design combines easy
management and optimum functioning with minimum maintenance.
The information contained in this manual is likely to go beyond what the average user needs to know to use
the microscope, however, it is provided to answer any queries that may arise.

Your new microscope combines high performance features, with an excellent degree of optical resolution
and clarity of image. It incorporates a mechanical stage that provides a travel range of 70mm x 50mm in X
and Y directions with a graduation of up to 0.1 mm, thus permitting the perfect positioning of the specimen.
Also included are objectives located on a ball bearing nosepiece allowing movement in both directions; a
precision coarse and fine focusing system; a moveable Abbe condenser with a numerical aperture of 1.25
N.A. and a built-in 12V/ 20W halogen variable light source.

These instructions should be read carefully before operating the microscope. They will permit you to use
your new microscope to its fullest capabilities. Terminology used to describe components and controls can
be found in the diagram on page 2.

These instructions are based on the assembly and use of the B1-220 model (Binocular) with
additional notes applying specifically to other models in the series.

Unpacking

All components of the microscope have been carefully packed to make sure they reach you in perfect
condition. We recommend that you do not discard any packing containers in case you need to return the
microscope, store it for long periods of time; or should it become necessary to transport it to a technical
service for any repair, or maintenance procedure.

The box should contain the following components, depending on the model:

• B1-211 (Monocular): Stand, Power cable, monocular head, eyepiece, eyepiece protector, four
objectives, condenser, sample holder mechanism, blue, green and yellow filter, fuse, dust cover, a
bottle of immersion oil and a 2mm hexagonal key.

• B1-220 (Binocular): Stand, Power cable, binocular head, two eyepieces, two eyepiece protectors,
four objectives, condenser, sample holder mechanism, blue, green and yellow filter, fuse, dust cover,
a bottle of immersion oil and a 2mm hexagonal key.

• B1-223 (Trinocular): Stand, Power cable, trinocular head, two eyepieces, two eyepiece protectors,
four objectives, condenser, sample holder mechanism, blue, green and yellow filter, fuse, dust cover,
a bottle of immersion oil and a 2mm hexagonal key.

Remove, and handle the microscope and all its components with extreme care.
Avoid touching the lenses of the optical elements and keep clear of contact with dust, water or other

contaminating agents, as they could stain, or damage the lens surface and affect the quality of the
image.

A. Place the microscope in an upright position on a flat, stable and clean surface.
B. Unscrew the black plastic protectors from the revolving nosepiece (3).
C. Remove the rest of the components from the box.

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Assembly

All the steps described for the assembly of the microscope must be undertaken with extreme care,
and without forcing the placement of the distinct parts and elements of the microscope.

A. Condenser (10): Turn the coarse

focus knob (19) until the stage (8)
reaches its highest position. Turn
the focussing knob on the
condenser (Fig.1) until the
condenser holder reaches its
lowest position. Unscrew the
knurled locking screw on the
condenser, and insert the
condenser in the mounting ring so
that the aperture of the diaphragm
(9) faces the front of the
microscope and so can be
adjusted easily. Once firmly
placed in the mounting ring, firmly
tighten the locking screw, and turn
the condenser focusing knob until
in its highest position.

Condense

r

set screw

Condenser
focusing
knob

Fig. 1

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B. Specimen Holder Mechanism (6): Turn the Coarse Focus Knob (20) until the stage (8) reaches its

lowest position. Unscrew the two knurled locking screws (18) of the specimen holder mechanism.
Place the mechanism on the stage with the moveable finger lever facing upwards (5) so that the
holes of the mechanism line up with the locking screws. Firmly screw the screws back into place.

C. Objectives (4): Ensure that the revolving nosepiece (3) is well positioned: i.e. that it does not revolve

freely, but rotates until a “click” is heard, showing that it is secure in place. Place objectives, starting
with the lowest magnification 4X in any of the holes in the revolving nosepiece. Next, place that of
the second lowest magnification 10X in the hole alongside, and so forth: the 40X objective, and
finally the 100X objective can be placed.

D. Head (15): Loosen the knurled locking screw on the head and remove the protective cover on the

collar where the head is placed. Insert the head, until it is well supported on the stand (17). Although
the head can be placed in any position, we recommend for your comfort that the eyepiece tubes (14)
face forward on the microscope. Firmly re-tighten the knurled locking screw.

E. Eyepieces (1): Remove the protective covers on the eyepiece tubes (14). Gently place the eyepieces

in the tubes without touching the lens surface.

• B1-211 and B1-212: Remove the

protective cover on the eyepiece tube.
Using a jewellers screwdriver, loosen the
tiny screw situated on the lateral of the
tube. Gently, and without touching the
surface of the lens, place the eyepiece in
the tube. Re-tighten the locking screw on
the eyepiece.

F. Filter: Swing out the filter holder horizontally

and place the blue filter in the filter holder
(11) located under the condenser (10). Make
sure that the filter is well positioned.

G. Power cable: Insert the power cable in the

connector at the lower back of the
microscope. (Fig. 1)

On switch

Fuse

Fig. 2

Warning: Before connecting the microscope to a

power source, check that the voltage coincides with
that on the microscope.

Operation

A. Starting up.

1. Before using the microscope, adjust the light intensity control (23) to minimum position. This
should be repeated every time the microscope is switched on or off to prolong the use of the
bulb.

2. Press switch to ON position. (Fig. 2)

3. Turn illumination control until the image is illuminated.

4. Light intensity must be adjusted according to the objective used, or the type of preparation to
be observed.

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