Millipore EZ-Pak Maintance Manual

TM

http://www.millipore.com

For units produced from February 1997 to

present (cat. no. EZDI SP0 01)

View previous manual on Worldwide Web

EZ--Pak

Membrane␣ Dispenser – Distributeur de membranes
Membran–Dispenser – Dispensador de membranas
Dispensatore di membrane – Membrane␣ Dispenser

 !"#$%&'

Operation␣ and␣ Maintenance␣ Instructions – Manuel␣ d'utilisation␣ et␣ d'entretien
Betriebs-␣ und␣ Wartungsanleitung – Instrucciones␣ de␣ utilización␣ y␣ mantenimiento

Manuale␣ operativo␣ e␣ di␣ manutenzione

 !"

ENGLISH

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EZ--Pak

Membrane Dispenser

Operation and Maintenance Instructions

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1

CONTENTS

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Page

1-Introduction 4
2-Component Parts 5
3-Start-up 6 to 8
4-Using the Membrane Dispenser 9
5-Maintenance 10
6-Specifications 10
7-Troubleshooting 11 to 13
8-Replacing Wear Parts 14 to 16
9-Ordering Information 17
10 - Warranty 18

Millipore Offices Worldwide

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3

1 INTRODUCTION

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Congratulations for your choice of EZ-PakTM membranes and dispenser
for microbiological analysis of liquid samples.

The EZ-Pak system consists of sterile membranes packed in a special
pleated band within a cartridge and specially designed equipment to
allow individual membrane dispensing.

EZ-Pak is designed to be used under standard «clean conditions»
and with traditional membrane filtration methods.

With EZ-Pak, three delicate manipulations are eliminated: opening
individual sterile envelopes, separating the membrane from its protective
papers and removing the fragile membranes from their packaging.

The EZ-Pak cartridges contain 150 black or white gridded 47-mm or
50-mm diameter membranes, and should be used with the Microfil

TM

filtration system for maximum ease of use, speed and cleanliness. Of
course, EZ-Pak is also an ideal complement to traditional filtration
equipment.

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4

2COMPONENT PARTS

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7 89210

6

5

4

3

2

11 12

1

FIGURE 1 - Assembly and component parts

1- Paper spool
2- Film/paper spool clips
3- Paper guide
4- Drive roller
5- Actuator
6- Plate
7- Separating bar

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8- Tensionning spring

(located under the back cover)

9- Film spool
10 - Metal cover
11 - Carrying handle

(moulded in the external case)

12 - Membrane cartridge holder

5

3START-UP

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1. Remove the dispenser from its packaging and place it on the work surface

with the dispensing mechanism facing you.

2. Check that all the component parts are undamaged and in place as shown

in Figure 1.

3. Open an EZ-Pak

cartridge

cut top cover and folding it over
backwards (see Fig. 2).

4. Insert the

so that the label is visible (see
Fig. 3).

Important operation:

5.

Fully depress the
proceeding to ensure correct
indexing of the
arrow on the
point vertically upwards.

by detaching the pre-

cartridge

membranes

in the

actuator

drive roller.

drive roller

holder

Fig.␣ 2

before

The

must

Caution: Avoid letting the

actuator
Slow the movement with the

spring back into place.

Fig.␣ 3

finger.

6. Remove the

separating the
the

plate

tab (see Fig. 4).

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6

metal cover

cover

tab from

by

Fig.␣ 4

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7. Pull out the band until the first

cell containing a membrane is
visible (see Fig. 5).

Note: The first cells on the band

do not contain membranes.

This
avoids wasting membranes
when installing the band.

8. Introduce the EZ-Pak band

under the

separating bar

. Pull
gently on the band to pass
3␣ empty cells under the

separating bar

.

Fig.␣ 5

9. Reinstall the

metal cover

by
inserting the pins in the
housings, then aligning the

cover

tab with the

plate

tab (see
Fig. 6). The locking ball should
audibly «snap» into place.

10. Peel away the transparent plas-

tic film from the paper backing
of the first cells up to the

separating bar.

11. Pull the backing paper through

the mechanism as shown in Fig­ure 1.

Important operation:

Make sure the red arrow on the
paper after the second fold is
located above the vertical arrow
on the

drive roller.

Engage the

backing paper on the sprockets.

Fig.␣ 6

Fig.␣ 7

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7

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12. Rotate the

clip

is horizontal. Remove the

paper spool clip.

paper spool

Place the

until the

backing paper on the spool and
secure it with the

clip

. If

necessary, manually rotate the

paper spool

clockwise to take

up any slack.

13. If necessary, slightly pull the

backing paper back from the

cover

toward the

holder

until
there is no slack left in the paper
between the

drive roller.

the

separating bar

and

Fig.␣ 8

14. Remove the

secure it with the
firmly held between the
Manually rotate the

15. Fully depress the

film spool clip

clip,

being careful not to twist the film. The film should be

clip

film spool

actuator

once then release it, slowing the upward movement

, place the end of the

film

on the

film spool

and spool.

clockwise to take up the slack (see Fig. 7).

and

with the finger.

16. Verify that the membrane band is correctly installed by checking that the

cell dispensed is approximately one-third open (the bluish seal should be
peeled off around one-third of the edge) and that there is no slack in either
the film or the backing paper.

The EZ-Pak membrane dispenser is now ready for use.

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8

4USING␣ THE␣ MEMBRANE␣ DISPENSER

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1. Position the dispenser on the left-hand side* of your workstation with the

Millipore logo directly facing you as shown in Figure 9.

2. To aseptically dispense a membrane, fully depress the

left* hand. Repeat this step if the cell released does not contain a mem­brane. The number 001 indicating the first membrane can be seen on the
film above the
actuator spring back into place. Slow the movement with the finger.

3. The membrane will be dispensed horizontally and held in place in the semi-

opened cell (see Fig. 9). Use a flamed and cooled forceps (see Fig.␣ 9) to
remove the membrane with the right hand
and setting it on a Microfil or conventional Millipore filtration support.

4. Proceed with filtration in the normal manner.

5. After dispensing the last membrane, pull off the

used packaging.

* invert if the operator is left handed

separating bar

. During these operations, avoid letting the

*

by pulling it straight out from the cell

clips

actuator

and remove the rolls of

with your

Fig.␣ 9

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9

5 MAINTENANCE

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The case surfaces may be cleaned using a mild detergent or decontaminated
with alcohol. No other maintenance is required.
If the outside case was exposed to UV rays often for relatively long periods, it
may have become slightly dull. Wiping it off with a cloth moistened with alcohol
will restore its original appearance almost completely.

Precautions

Avoid spraying liquids into the mechanism.

Do not autoclave.

Never use the

carrying handle

If it proves necessary to lubricate certain mechanical parts, use only thick
silicone grease, never oil, and be careful not to allow the grease to come
in contact with the spools and drive belts.

film spool

for this purpose.

to lift or carry the dispenser. Use only the

6SPECIFICATIONS

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Materials of construction

External case: Ultem®.
Internal case: Stainless steel.
Paint: Epoxy-baked.

Dimensions and Weight

Height: 240 mm. Width: 160 mm. Depth: 235 mm.
Weight empty: 2.4 kg.

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10

7 TROUBLESHOOTING

Send E-mail to Technical Service

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Symptom Probable cause Corrective action

Refer to Figures 10 and 11 on the page 13 for the list of component parts.

Membranes not dispensed correctly

1. The film is not separated

from the paper backing
on the
film is pulled by the
backing).

2. The paper backing

catches on the sprockets
(slack in the backing
between
paper spool).

3. There is slack in the

paper backing between
the

roller:

plate

edge (the

drive roller

plate

and the

The film is no longer
separated from the
backing at the
edge.

The film is separated
from the backing at
the

plate

edge.

and

drive

plate

Separate the film from the
paper backing up to the
separating bar. If the fault
persists, the
driving belt is worn

The

film

is worn.

The

film spool

is worn.

The

tensioning spring

not taut enough.

film spool

spool driving belt

driving belt

is

Replace the driving belts
and the

Replace the driving belts
and the

Replace the driving belts
and the

Replace the

spring

tensioning spring

tensioning spring

tensioning spring

tensioning

and the driving belts.

.

.

.

4. The

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actuator

drive the

The symptom persists,
but disappears when
the
all the way against
the stop.

The symptom persists
when the
depressed then raised
fully against the stop.

does not

drive roller:

actuator

actuator

is raised

is

The lever spring is no
longer taut enough.

The latch springs are
damaged.

Check whether the symptom
persists after fully
depressing the
all the way down against
the stop.

Replace the lever spring.

Replace the latch springs.
It is recommended that the

driving belts
spring

same time.

be replaced at the

pushbutton

and

tensioning

11

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Symptom Probable cause Corrective action

The

metal cover

is difficult to install or is not securely held in the

housing.

The locking ball is out of
adjustment.

Adjust the position of the
locking ball with a
screwdriver.

Removing a partially used band from the spools and reinstalling
it after repair:

1. Rotate the

paper spool

counterclockwise by two turns to slacken the paper

backing.

2. Remove the

3. Remove the paper backing by pulling it straight out of the

4. Take the paper backing off the sprockets on the

guide

.

paper spool clip

.

paper spool.

drive roller

and the

paper

5. To remove the film, repeat steps 1 to 3 above on the film spool.

6. Lay the film on the paper backing.

7. Cut the film and paper off at the fourth empty cell (the fourth cell ahead of

the first membrane).

8. Repeat steps 11 to 16 (page 7–8) to reinstall the band. To position the

backing paper (step 11), the vertical arrow must be located two sprocket
holes ahead of a fold (the closest fold is located to the left of the
arrowhead).

For additional information, contact your nearest Millipore office, specifying the
catalogue number and serial number on the nameplate under the dispenser.

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12

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Driving belts

Actuator

Lever

Rack positioning screw

Lever spring

Lever return

damper

Rack bar clip

Feet

FIGURE 10

Rack bar

Latch nodule

Actuator

Lever screw

Lever

Feet

FIGURE 11

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13

8 REPLACING WEAR PARTS

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Driving Belts and Latch Springs

These parts should be replaced together.

Tools

2.5 mm and 3 mm Allen keys
Latch spring installation tool
Set of latch belts and springs
Scissors or cutter
Cloth and cleaning agent

Procedure

1. Unscrew the 6 socket head screws with the 2.5 mm Allen key and

remove the inside cover (located on the side of the spools).

2. Remove the outside cover as follows:

Break the retaining tab located under the actuator with a screwdriver
or other strong pointed object and remove the actuator.

Remove the two feet by pushing the internal shaft and pulling on
the feet.

Unscrew the five socket head screws from the cover with the 3␣ mm
Allen key.

Push the lever down against the stop and hold it there.
Remove the cover.

3. Cut the two drive belts.

Replacing the Latch Springs

4. Lift the latch and cut the two retaining springs.

5. Remove the latch.

6. Engage the spring installation tool on the

7. Fit two new springs on the tool to place them in their grooves.

drive roller

.

8. Remove the installation tool.

9. Lift the two springs and fit the latch in its housing.

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14

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Replacing the Driving Belts

10. Clean the spool grooves with a clean cloth slightly moistened with a

cleaning agent (e.g. acetone).

11. Set the two belts on a flat surface, one inside the other.

12. Engage the larger belt (the outside one) on the

film spool

and

drive roller

13. Move the belt along the spools, fitting it over the sprockets, until it is in

the grooves.

14. Engage the second, smaller belt on the

paper spool

and

drive roller

.

15. Move the belt along the spools, fitting it over the sprockets, until it is in

the grooves.

16. Install the inside and outside covers by carrying out steps 1 and 2 of

page 14 in reverse order.

17. Check that the belts are correctly positioned by operating the

actuator

several times.

Tensioning Spring

The

tensioning spring

belts and springs.

should always be replaced at the same time as the latch

.

Tools

Adjustable wrench

Tensioning spring

Procedure

1. Unscrew the spring-nut and lock-nut located under the

plate

. Remove the

two washers and the old spring.

2. Install a new spring, the flat washer then the self-locking washer, the nut

and the lock-nut.

3. Check that the spring does not rub against the sides of the oblong hole.

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15

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Lever Spring

Tools

2.5 mm Allen key
3 mm Allen key
Lever spring

Procedure

1. Unscrew the 6 socket head screws with a 2.5 mm Allen key and remove

the inside cover (located on the side of the spools).

2. Remove the outside cover as follows:

Break the retaining tab located under the actuator with a screwdriver
or other strong pointed object and remove the actuator.

Remove the two feet by pushing the internal shaft and pulling on
the feet.

Unscrew the five socket head screws from the cover with the 3 mm
Allen key.

Push the lever down against the stop and hold it there.
Remove the cover.

3. The spring is then accessible and can be replaced without using any

tools.

4. Install the inside and outside covers by carrying out steps 1 and 2 on in

reverse order.

For additional information, contact your nearest Millipore office, specifying
the catalogue number and serial number on the nameplate under the dispen­ser.

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16

9 ORDERING INFORMATION

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Ordering Information

Description Catalogue No.

EZ-Pak membrane dispenser (Qty 1) EZDI SP0 01

Kit of spare parts including: EZDI KIT 01

- 3 actuators

- 1 clip

- 4 feet

- 2 sets of latch springs

- 2 sets of driving belts

- 2.5 mm and 3 mm Allen keys

- latch spring installation tool

- latch

- cover attaching screws

- 2 tensioning springs

EZ-Pak Membranes (4 cartridges, each containing 150 membranes)

EZ-Pak membranes, 0.45 µm, white, MSP0 008 13
gridded, 47 mm diameter, Q 600

EZ-Pak membranes, 0.45 µm, black, MSP0 008 14
gridded, 47 mm diameter, Q 600

EZ-Pak Membranes (150 membranes with 150 Microfil funnels)

EZ-Pak membranes, 0.45 µm, white, MZHA WG1 01
gridded, diameter 47 mm, Q150
and 6 packets of 25 Microfil funnels, 100 ml

EZ-Pak membranes, 0.45 µm, white, MZHA WG2 51
gridded, diameter 47 mm, Q150
and 5 packets of 30 Microfil funnels, 250 ml

EZ-Pak membranes, 0.45 µm, black, MZHA BG1 01
gridded, diameter 47 mm, Q150

and 6 packets of 25 Microfil funnels, 100 ml
EZ-Pak membranes, 0.45 µm, black, MZHA BG2 51

gridded, diameter 47 mm, Q150
and 5 packets of 30 Microfil funnels, 250 ml

The range of available membranes is growing rapidly. Contact your nearest
Millipore office for further information on the other types of EZ-Pak membra­nes or to place an order.

○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○

17

10 WARRANTY

○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○

Millipore warrants its products against defects in manufacturing and workmanship
for a period of one (1) year from the date of delivery, provided that they have
been used under the conditions described in this manual.

Millipore offers no other warranty neither explicit nor implicit, in particular no
warranty of merchantability or fitness for a particular purpose. The terms of this
warranty, as well as information, characteristics and descriptions of Millipore
products shown on brochures and catalogues published by the Company may
not be modified without express authorization signed by a duly qualified Millipore
representative. Any written or oral interpretation not in accordance with this
warranty or to the said publications shall be considered null and void.

In case of non-respect of the terms of the above-mentioned warranty, Millipore’s
sole obligation shall be to repair or replace, at its discretion, all or part of a
product found to be defective during the period of warranty, due to defects in
workmanship or manufacturing, on condition that the customer notifies the fault
immediately.

The present service obligation cannot be considered as not having been fulfilled
if Millipore has demonstrated its good faith in repairing or replacing any defective
Millipore product or part.

Millipore cannot be held responsible for prejudice due to non-operation or
damage to property where its products could have been the cause, nor
consequently be required to award indirect damages.

The terms of this warranty do not affect the buyer’s statutory rights.

○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○

18

Millipore␣ Worldwide␣ –␣ Millipore␣ dans␣ le␣ monde␣ –
Millipore␣ weltweit␣ –␣ Millipore␣ en␣ el␣ mundo␣ –
Millipore␣ nel␣ mondo –  !"#$$%&

○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○

AUSTRALIA

Millipore Australia Pty. Ltd.
A.C.N. 001 239 818
Private Bag No. 18
87–89 Mars Road
Lane Cove, N.S.W.2066
Tel. Toll-Free: (008) 222–111
In Sydney Area (02) 428–7333
FAX [phone]

AUSTRIA, CENTRAL EUROPE,
C.I.S., AFRICA, MIDDLE EAST
AND GULF

Millipore Ges.m.b.H.
Hietzinger Hauptstraße 145
A–1130 Wien, Austria
Tel. [phone]
Telex 131464 miliv a
FAX [phone]

BALTIC REPUBLICS

Millipore Oy
Lanttikatu 2
FIN–02770 Espoo
Finland
Tel. [phone]
FAX [phone]

BELGIUM AND
LUXEMBURG

Millipore S.A.–N.V.
Rue de la Fusée 60
Raketstraat 60
B–1130 Bruxelles–Brussels
Tel. [phone]
FAX [phone]

BRAZIL

Millipore Indústria
e Comércio Ltda.
Rua Prof. Campos
de Oliveira, 430
CEP 04675
São Paulo–SP
Tel. [phone]
Telex (011) 57751 MILY BR
FAX [phone]

CANADA

Millipore (Canada) Ltd.
36 Antares Drive/
Suite 700/
Nepean, Ontario, K2E 7W5
Tel. Toll-Free 1–800 Millipore
FAX [phone]

CHINA
PEOPLE’S REPUBLIC OF

Millipore China Ltd.
Room 302 Office Bldg.
Asia Jinjiang Hotel
8 Xinzhong Xijie Gongti
Bei Lu, Beijing (100027)
Tel. [phone]
FAX [phone]

Millipore China Ltd.
Room 615
China Hotel Office Tower
Linhua Lu
Guangzhou
Postal Code 510015
Tel. [phone]

○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○

CHINA
PEOPLE’S REPUBLIC OF

Millipore China Ltd.
Suite 2806, Union Building
100 Yan An Road (E)
Shanghai 200002, P.R.C.
Tel. [phone]
FAX [phone]

CZECH REPUBLIC

Millipore spol.s.r.o
Obchodní Zastoupení
Rícanova 21
CZ–169 00 Praha 6
Tel. [phone]
FAX [phone]

DENMARK

Millipore A/S
Baldersbuen 46
DK–2640 Hedehusene
Tel. [phone]
FAX [phone]

FINLAND

Millipore Oy
Lanttikatu 2
FIN–02770 Espoo
Tel. [phone]
FAX [phone]

FRANCE

Millipore S.A.
BP 307
F–78054 Saint–Quentin
Yvelines Cedex
Tel. [phone]
Telex 698 371 F
FAX [phone]

GERMANY

Millipore GmbH
Hauptstraße 87
D–65760 Eschbom
Tel. [phone]
Telex 40 72 704 mil d
FAX [phone]

HONG KONG

Millipore China Ltd.
Millipore Asia Ltd.
1406 Cityplaza 4
12 Taikoo Wan Road
Hong Kong
Tel. [phone]
Telex 331 40 MWBHK HX
FAX [phone]

HUNGARY

Millipore kft
Andor u. 47–49
H–1119 Budapest
Tel. [phone]
(1) 185 11 22
Telex 22 30 57
FAX [phone]

INDIA

Millipore (India) Pvt. Ltd.
50–A, 2nd Phase,
Ring Road Peenya
Bangalore 560 058
Tel. [phone], 396320
Telex 845–5089 MLPR IN
Cable: Millipore
FAX [phone]

ITALY

Millipore S.p.A.
Via B. Buozzi, 4
I–20090 Vimodrone MI
Tel. [phone]
FAX [phone]

Millipore S.p.A.
Via D. Sansotta 100
I–00144 Roma
Tel. [phone]
FAX [phone]

JAPAN

Nihon Millipore Ltd.
Dai 5 Koike Bldg. 3–12
Kitashinagawa 1–Chome
Shinagawa-Ku
Tokyo 140
Tel. [phone]
Telex 24948 MILLIPOR J
FAX [phone]

KOREA

Millipore Korea Ltd.
13F, Yusung Bldg.
706-25, Yoksam-Dong
Kangnam-Ku, Seoul 135–080
Tel. [phone]
FAX [phone]

MALAYSIA

Millipore Asia Ltd.
Malaysia Branch
B4 – 05/06, PJ. Industrial Park
Section 13 Jalan Kemajuan
46200 Petaling Jaya, Selangor
Tel. [phone]
FAX [phone]

MEXICO

Millipore S.A. de C.V.
Avenida Ingenieros Militares 85–PB
México 11230 D.F.
Tel. [phone]
FAX [phone]

THE NETHERLANDS

Millipore B.V.
Penningweg 33
Postbus 166
NL–4870 AD Etten-Leur (N.B.)
Tel. [phone]
Telex 54 840 milwa nl
FAX [phone]

NORWAY

Millipore AS
Postboks 39, Simensbraten
N–1107 Oslo
Tel. [phone]
FAX [phone]

POLAND

Millipore Sp.z.o.o.
Oddzial w Warszawie
Ul. Jasnodworska 7
PL–01–745 Warszawa
Tel. [phone] 31
Telex 825 422
FAX [phone]

PUERTO RICO

Millipore Corporation
Puerto Rico Branch
P.O. Box 11977
Cidra, Puerto Rico 00739
Tel. [phone]
FAX [phone]

SINGAPORE

Millipore Asia Ltd.
Singapore Branch
#07–01/02 Jumbo Industrial Bldg.
19 Kim Keat Road
Singapore 328804
Tel. [phone]
FAX [phone]

SPAIN AND PORTUGAL

Millipore Ibérica, S.A.
Avda. del Llano Castellano, 13–3°
E–28034 Madrid
Tel. [phone]
FAX [phone]

Millipore Ibérica, S.A.
Entenza, 28°
E–08015 Barcelona
Tel. [phone]
FAX [phone]

SWEDEN

Millipore AB
Box 7090
S–172 07 Sundbyberg
Tel. [phone]
FAX [phone]

SWITZERLAND

Millipore AG
Chriesbaumstrasse 6
CH–8604 Volketswil
Tel. [phone]
Telex 82 94 08 MILAG CH
FAX [phone]

Millipore SA
Avenue de Maillefer 43
CH–1052 Le Mont-sur-Lausanne
Tel. [phone]
FAX [phone]

TAIWAN

Millipore Asia Ltd.
Unit #707, 7/th, fl
136 Jen-Ai Road, Sec. 3
Taipei Tawai R.O.C.
Tel. [phone]
FAX [phone]

U.K. AND IRELAND

Millipore (U.K.) Ltd.
“The Boulevard”
Blackmoor Lane Watford,
Hertfordshire WD1 8YW
Tel. [phone]
Telex 24191 milpor g
FAX [phone]

U.S.A.

Millipore Corporation
P.O. Box 255
Bedford, MA 01730
Tel. Toll-Free
(including Massachusetts)
1-800 Millipore
or 1-800-645-5476
In Western States,
Alaska & Hawaii
800–632–2708
In Puerto Rico [phone]

IN ALL OTHER COUNTRIES

Millipore Intertech
80 Ashby Road
Bedford, MA 01730
U.S.A.
Tel. [phone]
FAX [phone]

Cat. No. PF 05600. Printed in France, 11/96. Copyright © 1996, Millipore Corporation, Bedford, MA, U.S.A., or affiliated company. Millipore is a registered
trademark of Millipore Corporation or an affiliated company. EZ-Pak and Microfil are trademarks of Millipore Corporation or an affiliated company.