Millipore 8003, 8010, 8050, 8200, 8400 User Manual

Stirred Ultraltration Cells

Models 8003, 8010, 8050, 8200, 8400

User Guide

Notice

The information in this document is subject to change
without notice and should not be construed as a
commitment by Millipore Corporation or an afliated
corporation. Neither Millipore Corporation nor any of its
afliated corporations assumes responsibility for any
errors that may appear in this document. This manual
is believed to be complete and accurate at the time of
publication. In no event shall Millipore Corporation or an
afliated corporation be liable for incidental or conse­quential damages in connection with or arising from the
use of this manual.

© 2008 MILLIPORE CORPORATION. PRINTED IN
THE UNITED STATES OF AMERICA. ALL RIGHTS
RESERVED.

Millipore and Amicon are registered trademarks of Millipore Corporation.
The M mark is a trademark of Millipore Corporation.
Cellosolve is a registered trademark of Union Carbide Chemicals &
Plastics Technology Corporation.
Tygon is a registered trademark of Saint-Gobain Performance Plastics
Corporation.
Saint-Gobain is a registered trademark of Compagnie de Saint-Gobain
Corporation.

99228, Rev. M, 08/08

Contents

Introduction ............................................. 1

Models Available ..................................... 1

Safety Features ...................................... 1

Model 8003 Parts List ............................. 2

Model 8010 Parts List ............................. 4

Model 8050 Parts List ............................. 6

Model 8200 Parts List ............................. 8

Model 8400 Parts List ........................... 10

Operating Modes .................................. 12

Unpacking the System ..........................13

Setting Up the System .......................... 14

Operating Guidelines ............................ 17

Limitations ............................................ 18

Cell Operation .......................................19

Shutting Down the System ....................21

Sterilization ...........................................21

Storage .................................................22

Maintenance ......................................... 22

Troubleshooting .................................... 23

Specications ....................................... 24

Materials of Construction ...................... 24

Compliance with the Pressure Equipment

Directive, 97/23/EC ...............................25

Product Ordering Information................ 26

Technical Assistance............................. 30

Standard Warranty ................................ 30

Introduction

Millipore stirred ultraltration cells are designed for
rapid concentration or purication of macromolecular
solutions in volumes from 3 to 400 mL. All models are
used in conjunction with a stirring table. The table is
magnetically coupled to a stirring bar, which maintains
uid movement during operation, thereby reducing the
negative effects of concentration polarization (i.e., the
buildup of concentrated solutes on the membrane).

Models Available

Model Capacity Millipore Cat. No.

8003 3 mL 5125

8010 10 mL 5121

8050 50 mL 5122

8200 200 mL 5123

8400 400 mL 5124

Safety Features

Cell has a built-in pressure-relief valve, preset to ■
nominal 75 psi (5.3 kg/cm2). The recommended
operating pressure is <55 psi (3.9 kg/cm

Cell remains xed in the retaining stand until ■
the pressure-relief valve is opened and the cap
depressed.

Stand provides a large base for improved cell stability. ■

2

).

2 www.millipore.com




























Model 8003 Parts List

A

B

L

K

C

D

E

M

N

F

G
H

I

J

Model 8003 Parts List, continued

Item Description Quantity Supplied

A Cap Assembly 1

B Pressure Relief Valve —

C O-Ring 2

D Stirrer Assembly 1

E Body 1

F O-Ring 2

G Membrane —

H Membrane Holder 1

I Elastomeric Tubing 4 in.

J Base 1

K Tube Fitting Assembly 2

L Tubing, Plastic 4 ft

M Stand Assembly 1

N O-Ring 2

3Stirred Ultraltration Cells

4 www.millipore.com



























Model 8010 Parts List

A

B

L

K

C

D

E

M

F

G

H

I

J

Model 8010 Parts List, continued

Item Description Quantity Supplied

A Cap Assembly 1

B Pressure Relief Valve —

C O-Ring 2

D Stirrer Assembly 1

E Body 1

F O-Ring 2

G Membrane —

H Membrane Holder 1

I Elastomeric Tubing 4 in.

J Base 1

K Tube Fitting Assembly 2

L Tubing, Plastic 4 ft

M Stand Assembly 1

5Stirred Ultraltration Cells

6 www.millipore.com



























Model 8050 Parts List

A

B

L

C

D

K

E

M

F

G

H

I

J

Model 8050 Parts List, continued

Item Description Quantity Supplied

A Cap Assembly 1

B Pressure Relief Valve —

C O-Ring 2

D Stirrer Assembly 1

E Body 1

F O-Ring 2

G Membrane —

H Membrane Holder 1

I Elastomeric Tubing 4 in.

J Base 1

K Tube Fitting Assembly 2

L Tubing, Plastic 4 ft

M Stand Assembly 1

7Stirred Ultraltration Cells