Mettler Toledo L-2XLS+, L-100XLS+, L-200XLS+, L-300XLS+, L-1000XLS+ Operating Instructions Manual

Operating Instructions

Pipet-Lite™ XLS+

™

Manual Pipettes with RFID

Single-channel Multichannel Adjustable Spacer

2

• Single channel models with LTS or universal-fit shafts

• Multichannel models with LTS

• Adjustable spacer multichannel models with LTS

• RFID enabled, all models

Table of Contents

1.1 Introduction ........................................................................................................3

1.2 Setting Volume ....................................................................................................4

1.3 Tip Selection and Mounting ...................................................................................6

1.4 Tip Immersion Depth ............................................................................................6

1.5 Operation ...........................................................................................................7

1.6 Tip Ejector Arm Removal ......................................................................................8

1.7 Storage ..............................................................................................................8

1.8 Care and Maintenance .........................................................................................8

1.9 Autoclaving ......................................................................................................10

1.10 Upgrading your Pipette to XLS+ ...........................................................................10

1.11 Troubleshooting ................................................................................................11

1.12 Service, Calibration, and Repair ..........................................................................11

1.13 Replacement Parts ............................................................................................12

2.1 RFID (Radio-Frequency Identication) .................................................................14

3.1 Pipet-Lite XLS+ Multichannel Pipettes...................................................................14

4.1 Pipet-Lite XLS Adjustable Spacing Pipettes ............................................................16

5.1 Specications – All Models ............................................................ inside back cover

Contents of box

• Pipette model as ordered

• Conformance Certicate / Warranty Card

If any item is missing please call 800-4-RAININ (800-472-4646) in the US, or contact your

local MT ofce or distributor.

Rainin, Pipet-Lite, XLS, XLS+, LTS, LiteTouch, Hang-Ups are trademarks of Rainin Instrument, LLC. Pipet-Lite pipettes
are manufactured under U.S. Patent Nos. 5,614,153; 5,700,959; and 5,849,248. For use under U.S. patents
6,168,761 B1; 6,171,553 B1; and D426,643.

©2017 Mettler-Toledo Rainin, LLC. No part of this manual may be copied without the express written permission of
Mettler-Toledo Rainin, LLC.

Pipet-Lite XLS and XLS+

Manual Pipettes with RFID

Pipet-Lite XLS

3

1.1 Introduction

Rainin’s Pipet-Lite XLS and XLS+ family is continually

being upgraded with new features to further improve
ergonomics while maintaining high accuracy and perfor­mance. The pipette is available in several versions, out­lined below; this manual will describe all models.

Single-channel
11 models for volumes from 0.1 µL to 20 mL

Multichannel

12 models for volumes from 1 µL to 1200 µL,
including 8 and 12 channels

Adjustable-spacer

5 models for volumes from 5 µL to 1200 µL
including 6 and 8 channels, with nozzle spacing
adjustable from 9-19 or 9-14 mm.

Spring forces are reduced even further over previous

models. With a contoured handle with ngerhook, the

pipette is designed for maximum comfort and enhanced
performance.

New enhancements for Pipet-Lite XLS+ single channel

include low stiction* seals for incredibly smooth opera­tion and a tough high-density polymer tip ejector that is
corrosion free and easy to release in one smooth action.

Pipet-Lite XLS+ multichannels are enhanced with low

stiction seals and are up to 35% lighter than the previous
model with even lower plunger forces.

In models with LTS™ shafts (L-model single channels,

all multichannels and adjustable spacers), the patented
LTS LiteTouch™ Tip Ejection System reduces tip ejection
forces by up to 85% in the single-channel L models, and
provides absolutely consistent sample pickup across all
channels in multichannel models, as well as reducing tip
ejection force.

Finally, all models contain an RFID tag (radio-frequency

identication) for facilitating calibration management

when using the optional SmartStand and EDPAM soft­ware.

*stiction = static friction

Figure 1: Pipet-Lite XLS+

PLUNGER BUTTON
VOLUME SET

VOLUME LOCK

COUPLING NUT

DISPOSABLE
TIP

SHAFT

RFID IN
FINGER-HOOK

VOLUME
DISPLAY

RUGGED
HIGH-IMPACT

LOW THERMAL

CONDUCTIVITY
POLYMER

TIP EJECTOR

TIP EJECTOR
BUTTON

4

Single-Channel

1.2 Setting Volume

1. Turn the volume lock lever to the “unlock” position so the volume setting mechanism is un-

locked and free to turn. See left image in Figure 2.

UNLOCK LOCK

2. With the mechanism unlocked, orient the pipette so you are looking at the volume indicator,
then rotate the plunger button to change volume – counter-clockwise to increase, and clock-

wise to decrease volume.

Read the volume indicator from the top down:

2–20 µL: Black digits indicates µL. Red digits – tenths, hundredths of µL.

100–300µl: All digits black – µL.
1000–5000 µL: red digit – mL. Black digits– tenths, hundedths of mL.
10 mL and 20 mL: Red digit – mL. Black digits – tenths of mL.

3. To eliminate errors due to mechanical backlash: when setting the desired volume, rst turn the
knob 1⁄3 turn above the desired volume. Then turn the knob slowly clockwise until the desired

volume is displayed. Always dial down to the desired volume.

4. Turn the volume lock to the “lock” position (Fig. 2 above) to prevent changes to the volume
setting. Some volumes for the 200 µL model are shown in Figure 4.

Figure 2: Volume Lock Control

Figure 3: Volume Indicator by Pipette Model

Figure 4: Example volumes

5

Volume ranges and increments for each model are shown below:

Single-Channel

Volume Adjustable Range µL Recommended Range µL Increment µL
2 µL 0 to 2 0.1 to 2 0.002

10 µL 0 to 10 0.5 to 10 0.02
20 µL 0 to 20 2 to 20 0.02
100 µL 0 to 100 10 to 100 0.2
200 µL 0 to 200 20 to 200 0.2
300 µL 0 to 300 20 to 300 0.5
1000 µL 0 to 1000 100 to 1000 2.0
2000 µL 0 to 2000 200 to 2000 2.0
5000 µL 0 to 5000 500 to 5000 5.0
10 mL 0 to 10 mL 1 mL to 10 mL 20.0
20 mL 0 to 20 mL 2 mL to 20 mL 20.0

Multichannel and Adjustable-Spacers

Volume Adjustable Range µL Recommended Range µL Increment µL

10 µL 0 to 10 0.5 to 10 0.02
20 µL 0 to 20 2 to 20 0.02
50 µL 0 to 50 5 to 50 0.05
100 µL 0 to 100 10 to 100 0.2
200 µL 0 to 200 20 to 200 0.2
300 µL 0 to 300 20 to 300 0.5
1200 µL 0 to 1200 100 to 1200 2.0

Filter

The 5000 µL, 10 mL, and 20 mL pipettes use a lter in the end of the shaft to help prevent

liquid entering the shaft and contaminating the piston, should the plunger snap up during

aspiration. Using a lter is recommended when pipetting large volumes. Replace the lter if it

gets wet.

The 5000 µL and 10 mL pipettes use the same lter, as shown in the diagram above:
5000 µL: small diameter into the shaft. 10 mL: large diameter into the shaft.
Filter part numbers are 17001944 (pack of 100) and
17001945 (pack of 1000).

The lter for the 20 mL model is a cylinder.
Part numbers: 17001951 (pack of 100),
17001952 (pack of 500).

Figure 5: Filter orientation

6

Single-Channel

1.3 Tip Selection and Mounting

Rainin pipettes and tips are designed together as a pipetting system, both in LTS and traditio-

nal versions. All Rainin tips are BioClean and totally inert, so you can be assured of the best
pipetting results.

Rainin pipettes are calibrated with Rainin tips, and performance to published specications

can only be guaranteed when Rainin tips are used.

To mount a tip, press the pipette shaft into the end of the tip with light force. With both LTS and

traditional versions, the tip will seal properly on the shaft with minimal force — do not use
more force than is required.

1.4 Tip Immersion Depth

The recommended depth for tip insertion into the sample for each model is shown below.

Nominal Volume Volume Range Immersion Depth

2 µL 0.1 - 2 µL 1-2 mm
10 µL 0.5 - 10 µL 1-2 mm
20 µL 2 - 20 µL 2 - 3 mm
100 µL 10 - 100 µL 2 - 3 mm
200 µL 20 - 200 µL 3 - 6 mm
300 µL 20 - 300 µL 3 - 6 mm
1000 µL 100 - 1000 µL 3 - 6 mm
2000 µL 200 - 2000 µL 3 - 6 mm
5000 µL 500 - 5000 µL 6 - 10 mm
10 mL 1 mL - 10 mL 6 - 10 mm
20 mL 2 mL - 20 mL 6 - 10 mm

Tip immersion depth is critical and should not be exceeded, or the volume measured may be

inaccurate, possibly out of specication. The tip angle is also important – the pipette should

always be used in a position within 20 degrees of vertical. See Figure 6 below.

TIP

ANGLE

Figure 6: Tip Immersion Depth and Tip Angle