This manual describes the setup, function, operation and maintenance of CO2 incubators
of the types INCO and INCOmed with a chamber volume of 108, 153 and 246 litres. In this
manual, the term INCO is used for INCO as well as INCOmed for the purpose of simplification.
Distinctions are explicitly pointed out.
This manual is intended for use by the trained staff of the operator in charge of operating
and/or maintaining the incubator. If you are asked to work on the incubator, you should read
this manual carefully before starting work on the unit. Familiarise yourself with the safety
regulations. Only perform the work that is described in this manual. If there is something you
don't understand, or certain information is missing, ask your superior or get in touch with the
manufacturer. Do not do anything without authorisation.
Contents
The INCO incubator is available with different fittings: as a basic model, and with six different
additional modules. The technical fittings and functional range of the basic model and the
individual additional modules are described from page 11 .
If specific equipment features or functions are available only with one of the additional modules, this is pointed out in the relevant sections in this manual.
Due to individual fittings, depictions in this manual may be different from the actual appearance.
Other documents that you must read:
► for service and repair work (see page 63 ) – a separate service manual
Storage and Forwarding
This instruction manual belongs with the incubator and should always be stored so that those
who work on the incubator have access to it. It is the responsibility of the operator to ensure
that persons who work on or who will work on the incubator are informed as to the whereabouts of this instruction manual. We recommend that it is always stored in a protected location close to the incubator. Make sure that the instruction manual is not damaged by heat or
damp. If the incubator is sold on or transported and then set up again at a different location,
this instruction manual must go with it.
3
Content
Content
1. Safety regulations 6
1.1 Terms and icons used .......................................................................................................... 6
1.2 Product safety and dangers ................................................................................................ 7
1.3 Requirements of the operating personnel .......................................................................... 8
1.4 Responsibility of the owner ................................................................................................. 8
1.5 Changes and conversions ....................................................................................................8
1.6 Behaviour in case of malfunctions and irregularities .......................................................... 8
1.7 What to do in case of accidents .......................................................................................... 9
1.8 Switching off incubator in an emergency ........................................................................... 9
In this manual, certain common terms and icons are used to warn you of dangers or to give
you notes that are important in avoiding injury or damage. Observe and follow these notes
and regulations to avoid accidents and damage. These terms and icons are explained below.
1.1.1 Terms used
"Warning"is always used whenever you or somebody else could be injured if you do
not observe the accompanying safety regulation.
"Caution"is used for information that is important for avoiding damage.
1.1.2 Icons used
Prohibited sign (forbidding an action)
Do not tilt
appliance
Warning icons (warning of a danger)
Danger of
electrical shock
Warning of gas
bottles
Regulation signs (stipulate an action)
Disconnect the
mains plug
Do not lift appliance
Explosive
atmosphere
heat/hot surfacesGas
Wear gloves
Danger of frostbite/cold burns
Wear safety
shoes
Other icons
6
Observe
information in
separate manual
Information on
first aid
Two or more
persons required
First Aid:
Rinse eyes out
Important or
useful additional information
Safety regulations
1.2 Product safety and dangers
Incubators of the INCO type are technically well-developed, manufactured using high-quality
materials and tested for many hours in the factory. They contain the latest technology and
comply with recognised technical safety regulations. But there are still dangers involved, even
when the appliance is used as intended. These dangers are described below.
Warning!
After removing covers, live parts may be exposed. You may receive
an electric shock if you touch these parts. Disconnect the mains plug
before removing any covers. Any work inside the unit may only be
performed by qualified electricians.
Warning!
When loading the chamber with an unsuitable load, poisonous or
explosive vapours or gases may be produced. This could cause the
chamber to explode, and people could be badly injured or poisoned.
The chamber may only be loaded with materials/test objects which
do not form any poisonous or explosive vapours when heated up
(see also Chapter 2.7 Intended use on page 14 ).
Warning!
Danger of suffocation. In high concentrations, CO
and N2 can have
2
a suffocating effect. In normal operation, the incubator gives off
small amounts of CO
and – if equipped with the O2 module – N2
2
to its environment. You should therefore ensure that the room in
which it is installed is properly ventilated. If a gas bottle is not connected, or is empty, always close the stop valve or pressure reducer
on the bottle.
Warning!
High concentrations of CO
contact with CO
gas to the eyes and skin.
2
can cause cold burns or frostbite. Avoid
2
Warning!
CO
gas bottles may burst or explode at high temperature. Do not
2
use naked flames in the vicinity of the gas bottles. Store gas bottles
at lower than 50 °C in a well-ventilated location. Prevent water from
penetrating, as well as backflow into the gas bottles. It is essential
that you read the safety notes and regulations of the gas suppliers.
CO
and N
2
are not dangerous substances in terms of the German Ordinance on Hazardous
2
Substances (GefStoffV). You should nevertheless familiarise yourself with the applicable safety
regulations prior to handling such gas bottles.
7
Safety regulations
1.3 Requirements of the operating personnel
The incubator may only be operated and maintained by persons who are of legal age, and
who have received instructions for the incubator. Personnel who are to be trained, instructed
or who are undergoing general training may only be active on the incubator under the continuous supervision of an experienced person.
The incubator may only be transported by persons (fork-lift truck, manual pallet jack), who are
trained for this work and who know the corresponding safety regulations.
Repairs may only be performed by qualified electricians. In this case the regulations in the
separate service manual must be observed.
1.4 Responsibility of the owner
The owner of the incubator
► is responsible for the flawless condition of the incubator and for the incubator being oper-
ated in accordance with its intended use (see page 14 );
► is responsible for ensuring that persons who are to operate or service the incubator are
qualified to do this, have received instructions about the incubator and are familiar with
this operating manual;
► must know about the applicable regulations, requirements and work protection regula-
tions, and train staff acordingly;
► is responsible for ensuring that unauthorised persons have no access to the incubator;► is responsible for ensuring that the maintenance plan is adhered to and that maintenance
and repair work is properly carried out (see page 63 );
► ensures, for example through corresponding instructions and inspections, that the incuba-
tor and its surroundings are kept clean and tidy;
► is responsible for ensuring that personal protective clothing is worn by operating person-
nel, e.g. work clothes, safety shoes, protective gloves.
1.5 Changes and conversions
No independent conversions or alterations may be made to the incubator. No parts may be
added or inserted which have not been approved by the manufacturer.
Independent conversions or alterations result in the EC declaration of conformity (see page
16 ) losing its validity, and the incubator may no longer be operated.
The manufaturer is not liable for any damage, danger or injuries that result from independent
conversions or alterations, or from non-observation of the regulations in this manual.
1.6 Behaviour in case of malfunctions and irregularities
The incubator may only be used when in a flawless condition. If you as the operator notice
irregularities, malfunctions or damage, immediately put the incubator out of service (see
Chapter 1.8 ) and inform your superiors.
You can find information on eliminating malfunctions from page 40 .
8
Safety regulations
1.7 What to do in case of accidents
1. Keep calm. Act resolutely and with consideration. Pay attention to your own
safety.
2. Switch off the incubator and close the valves on the gas bottle.
3. Call a doctor.
4. Initiate first aid measures. If available: Call a trained first aid helper.
In case of contact with CO2 to the eyes and skin:
Rinse eyes out with water for at least 15 minutes. With cold burns, rinse with water
for at least 15 minutes. Cover over in a sterile manner. Call a doctor.
Inhaling CO2 or N2:
High concentrations can cause suffocation. Symptoms may include a loss of mobility and
consciousness. The victim is not aware of suffocating.
Low concentrations of CO
Anyone affected should breathe fresh air, using a breathing device independent of recircu-
lating air. Keep the person warm and calm. Call a doctor. In case of respiratory arrest, use
artificial respiration.
can cause accelerated breathing and headaches.
2
If gas is escaping:
Leave the room immediately, warn others and ventilate the room. If you re-enter the room,
use an autonomous breathing device (independent of ambient air) if it has not been established that the atmosphere is harmless.
1.8 Switching off incubator in an emergency
► Push main switch on front side of appliance
( Fig. 1 ).
► Close the valves on the gas bottle.
Fig. 1 Switch off incubator by
pressing the main switch
9
Design and Function
2. Design and Function
2.1 Design
1
10
11
12
13
2
3
18
4
5
6
7
17
8
16
9
15
14
Fig. 2 Design of INCO incubators
1 Controller/control panel (see page 29 )
2 Set key
3 Push/turn control
4 Connections on the rear of the oven
(see page 13 )
5 Heating ribs (see page 11 )
6 Pt100 temperature sensors
7 Humidity sensor
8 Glass door
9 Chamber seal
10 Sliding shelf
11 Door seal
12 Ventilator/inlet opening
13 Heating ribs
14 Adjustable feet
15 Nameplate (beneath door, see page 18 )
16 Door knob (see page 27 )
17 Door
18 Chip card reader
10
Design and Function
2.2 Function
The air in the incubator is heated up by an all-round heater with a large surface ( Fig. 3 , No. 1).
The CO
has a much higher specific weight than air, the gas is let into the working chamber above the
interior fan (2). The turbulence-free interior ventilation (3) ensures a uniform distribution of
the gases, creating a homogenous atmosphere.
In the basic version, humidification takes place by means of water trays (4). To avoid uncontrolled condensation, dosed fresh air is piped into the interior. If the appliance is equipped
with a humidity module, humidification takes place via a hot-air generator, which allows
water to evaporate at a set rate. The sterile hot air is let into the interior above the fan and is
mixed with the air current (5).
and/or N2 gas is introduced into the working chamber via a sterile filter. Because CO2
2
1
5
1
2
3
4
1
4
1
11
1
Fig. 3 How INCO incubators work
2.3 Optional extras
2.3.1 Basic equipment
► Electronic fuzzy-supported PID process controller with pulse width modulation and per-
manent performance adjustments and time-saving self-diagnosis system to quickly locate
errors (see page 41 )
► All-round heater with larger surface area, with additional thermal conduction layer (see
Fig. 3 )
► Capacitive humidity sensor► Humidity control (fresh air via sterile filter) ensures that setpoint humidity is quickly
reached and guarantees short recovery times, while avoiding condensation formation
► Homogenous atmosphere and temperature distribution through encapsulated, turbu-
lence-free ventilation system
► STERICard for fully automatic sterilisation process control for hot air sterilisation of appli-
ance, including sensors and fan rotor (see page 51 )
11
Design and Function
► Digitalised electronic CO
with self-diagnosis system and acoustic error display, air pressure compensation
control with automatic zero position, NDIR measuring system
2
► Language settings (see page 46 )► Alphanumeric text display► Integrated week time switch with grup function (e.g. each working day) (see page 33 )► Retracting push-turn control for simple operation of appliance (see page 28 )► Two separate Pt100 temperature sensors DIN Class A in a 4-wire circuit for control and
monitoring
► Digital monitoring control for overtemperature, undertemperature and automatic
setpoint following (ASF) (see page 50 )
► Mechanical temperature limiter (TB protection class 1, see page 47 )► Monitoring relay to switch off heater in case of error► Optical alarm display► Acoustic signal messages if temperature or CO
if gas bottle is empty (see page 40 )
► Calibration of temperature, humidity, CO
rate PC (see from page 53 )
and O2 possible on the device without a sepa-
2
limits are crossed, when door is open and
2
2.3.2 Optionally available additional modules
Comfort module:
► Two gas connections with quick release connectors (see page 13 )► Automatic switch-over of gas bottles
Hygiene module
► Electro-polished, seamless laser-welded chamber
Communication module
► Logging option of temperature, CO2 and relative humidity via computer/laptop► Internal log memory with 1024 kB as ring memory for all setpoint and actual values, errors
and settings in real time and with date, logging approx. 3 months at 1 minute storage
interval (see page 62 )
► Parallel printer port (PCL3-compatible) for printing out log data (see page 13 and page 45 )► optionally USB, Ethernet, RS-232 or RS-485 interface (see from page 58 )
CO2 module
► Extended adjustment range from 0 to 20 %► 3-point calibration (5 %, 10 %, 15 % CO
O2 module
)
2
► Control of oxygen concentration through the introduction of nitrogen (N
range 1 % to 20 % O
(not in combination with Comfort or Premium module)
2
2
Premium module
► Includes comfort, hygiene, communication and CO
modules
2
Humidity module
► Active microprocessor humidification and dehumidification control (40-97 % rh)
12
); adjustment
Design and Function
2.4 Material
For the outer housing, MEMMERT uses stainless steel (W.St.No. 1.4016), and for the interior,
stainless steel (W.St.No. 1.4301), which stands out through its high stability, optimal hygienic
properties and corrosion-resistance towards many (but not all!) chemical compounds (caution
for example with chlorine compounds). The chamber load for the appliance must be carefully
checked with respect to chemical compatibility with the materials mentioned.
A material resistance table can be requested from the manufacturer.
2.5 Electrical equipment
► Operating voltage: See nameplate (page 18 ), 50/60 Hz► Current consumption: See nameplate (page 18 )► Protection class 1, i.e. operating insulation with safety earth terminal in accordance with
EN 61010
► Protection type IP 20 acc. to EN 60 529► Interference-suppressed acc. to EN 55011 class B► Appliance fuse: Fusible link 250 V/15 A quick-blow► The temperature controller is protected with a miniature fuse 100 mA (200 mA at 115 V)► For models with a humidity model the CO
fuse
controller is protected with a 6.3 A miniature
2
2.6 Connections
1
2
H2O
N2
7
In
3
4
5
6
Fig. 4 Connections on rear of appliance
1 Water connection (only for model with humidity module)
2 Mains lead
3 USB connection (only for models with communication or premium modules;
alternatively Ethernet, RS-232 or RS-485 connection) (details from page 58 )
4 Printer connection (only for models with communication or premium modules) (see page
45 )
5 CO
6 CO
connection to main gas bottle
2
connection to reserve gas bottle (only for models with CO2 or premium modules)
2
7 N2 connection (quick-release connector, only if equipped with O2 module)
13
Design and Function
2.6.1 Electrical connection
Observe the country-specific regulations when making connections (e. g. in Germany DIN VDE
0100 with residual current device).
This appliance is intended for operation on an electrical power system with a system impedance Z
at the point of transfer (service line) of a maximum of 0.292 ohm. The operator must
max
ensure that the incubator is operated only on an electrical power system that meets these
requirements. If necessary, you can ask your local energy supply company what the system
impedance is.
2.6.2 Connection of external appliances
Only appliances may be connected externally (depending on the model, USB, RS 232, RS 485,
Ethernet, printer) whose interfaces comply with the requirements for safety extra-low voltage
(e.g. PC).
2.6.3 Gas connection
The oven can be connected with the supplied compressed air hose via a pressure regulator
with gas bottle monitor (DIN 8546) to a CO
gas supply.
compressed gas bottle or directly to a central CO2
2
For models with the CO
can be connected. If equipped with the O
of a second CO
gas bottle ( Fig. 4 ).
2
or premium modules, two gas bottles with quick release connectors
2
module, an N2 gas bottle can be connected instead
2
The pre-pressure must not exceed 1.2 bar. A value between 0.8 and 1 bar is considered to be
an ideal value.
2.6.4 Water connection
For models with a humidity module, the incubator can be connected with the supplied hose
to the also supplied water supply tank.
2.7 Intended use
INCO incubators may only be used for incubating cell cultures or similar. Any other use is
improper, and may result in hazards and damage.
The incubators are not explosion-proof (they do not comply with workplace health & safety
regulation VBG 24). The chambers may only be loaded with materials and substances which
cannot produce any toxic or explosive vapours at temperature ranges up to 50 ºC, and which
themselves cannot explode, burst or ignite.
The incubators may not be used for drying, vaporising and branding paints or similar materials, the solvents of which could form an explosive mixture when combined with air. If there is
any doubt as to the composition of materials, they must not be loaded into the incubator. Potentially explosive gas-air mixtures must not be produced, either in the interior of the chamber
or in the direct vicinity of the appliance.
The incubator may not be used for sterilisation purposes. It is not a steriliser with respect to
the Law on Medical Devices.
Only gas bottles with a pressure regulator may be connected to the gas connections of the
oven. Introducing other gases or materials than CO
14
or N2 is not permitted.
2
Design and Function
2.7.1 Intended use of the INCOmed
For INCOmed incubators, which are subject to the 93/42/EEC guideline (Council Directive on
the approximation of the laws of the Member States relating to medical devices), the intended
use is defined as follows:
The CO2 incubator INCO med is intended for the creation and maintenance of constant environmental conditions for application in the field of in vitro fertilisation (IVF), especially for the
incubation of oocytes, spermatozoa and zygotes in special culture dishes for IVF application as
well as for gene expression and the biosynthesis of RNA and proteins.
15
Design and Function
2.8 EC Declaration of Conformity
EC Declaration of Conformity
Manufacturer´s name and address: MEMMERT GmbH + Co. KG
Äußere Rittersbacher Straße 38
D-91126 Schwabach
Product: CO2 Incubator
Type: INCO 2
Sizes: 108 l / 153 l / 246 l
Nominal voltage: AC 230 V 50/60 Hz
The designated product is in conformity with the European EMC-Directive
2004/108/EEC
including amendments
Council Directive of 03 May 1989 on the approximation of the laws of the Member States relating to
electromagnetic compatibility.
Full compliance with the standards listed below proves the conformity of the designated product with the essential
protection requirements of the above-mentioned EC Directive:
DIN EN 61326-1:2006-10 EN 61326-1:2006
DIN EN 61000-3-11:2001-04 EN 61000-3-11 :2000
The designated product is in conformity with the European Low Voltage Directive
2006/95/EEC
Council Directive on the approximation of the laws of the Member States relating to Electrical
equipment for use within certain voltage limits.
Full compliance with the standards listed below proves the conformity of the designated product with the essential
protection requirements of the above-mentioned EC Directive:
DIN EN 61 010-1 (VDE 0411 part 1):2002-08 EN 61 010-1:2001
DIN EN 61 010-2-010 (VDE 0411 part 2-010):2004-06 EN 61 010-2-010:2003
Schwabach, 22.06.10
______________________________
(Legally binding signature of the issuer)
This declaration certifies compliance with the above mentioned directives but does not include a property assurance. The
safety note given in the product documentation which are part of the supply, must be observed.
including amendments
16
Modelljahr 2006 D10318 / 22.06.10
Design and Function
Manufacturer's name and address:
MEMMERT GmbH + Co. KG
Äußere Rittersbacher Straße 38
91126 Schwabach, Germany
Product:
Incubators
Type:
INCO med
Sizes:
108 l / 153 l / 246 l
Rated voltage:
AC 230 V 50/60 Hz
EC Declaration of Conformity
This product complies with the provisions of the directive:
93/42/EEC
including annex and revisions
Council Directive on the approximation of the laws of the Member States relating to medical
devices of 14 June 1993 (Official Journal of the EC No. L 169, page 1 of 12 July 1993)
Schwabach, Germany, 01/11/2012
legally binding signature of the manufacturer)
(
This declaration states the compliance with the above Directives, however, does not provide any warranted properties.
The safety instructions in the supplied documents have to be observed.
D24002
17
Design and Function
2.9 Designation (nameplate)
The nameplate ( Fig. 5 ) provides information about the appliance model, manufacturer and
technical data. It is attached to the front of the appliance, on the right beneath the door (see
page 10 ).
1
2
3
4
5
6
Fig. 5 Nameplate
1 Type designation
2 Operating voltage
3 Applied standard
4 Protection type
5 CE conformity
Typ: INC 108 F.-Nr.: 0109.0088
230 V
DIN12880-Kl.3.1 Nenntemp.: 50 °C
~
4.4 A 50/60 Hz 1000 W
6 Address of manufacturer
7 Disposal note
8 Temperature range
9 Connection / performance values
10 Factory number
due to adjustable feet) [mm]
Appliance depth F* (includ-
ing door handle) [mm]
Chamber volume [litres]108153246
Weight [kg]7090110
Performance [W]100015002000
Max. number of sliding
shelves half size / full size
108153246
778920938
590690790
-/4-/62 x 6/6
18
Design and Function
Model
* See Fig. 6 on page 20 .
Max. load per sliding shelf
[kg]
Max. load per appliance [kg]404060
TemperatureTemperature recording by means of Pt100 in a 4-wire circuit
Adjustment range: Normal mode: 20 °C to 50 °C
Sterilisation mode: 160 °C (4 hours) via STERICard
Adjustment precision: 0.1 °C
Control range: from 8 °C above room temperature to 50 °C
Variation (time): max. ±0,1 °C at 37 °C
Variation (spatial): max. ±0.3 °C at 37 °C
HumidityThe relative humidity in the chamber is measured by a capac-
itive humidity sensor and displayed digitally in percent.
The measurement precision of the humidity sensor is 1 % rh
108153246
151515
► Adjustment range: 88 to 97 % rh (for models with
The O2 content is determined with a long-lasting, maintenance-free zirconium dioxide sensor, constantly controlled by
a microprocessor and displayed digitally in percent
► The incubator may only be used in enclosed rooms and under the following environmental
conditions:
Ambient temperature: 5 ºC to 35 ºC
Humidity: max. 80 % not condensing
Degree of pollution: 2
Altitude of installation max. 3,000 m above sea level
► The incubator may not be used in areas where there is a risk of explosions. The ambient air
must not contain any explosive dusts, gases, vapours or gas-air mixtures. The incubator is
not explosion-proof.
► Heavy dust production or aggressive vapours in the vicinity of the appliance could lead to
sedimentation in the chamber interior and as a consequence, could result in short circuits
or damage to electrical parts. For this reason, sufficient measures should be taken to
prevent large clouds of dust or aggressive vapours from developing.
2.12 Accessories included
For incubators with basic fittings:
► Two (INCO 108) or three (INCO 153 and 246) sliding shelves► Gas pressure hose► Water tray (one for INCO 108 and 153 incubators, two for INCO 246 incubators)► Sterilisation chipcard
Additionally, for models with humidity module:
► Water supply tank and connection hose
Additionally, for models with CO2 or premium module:
► Second gas pressure hose with quick-release connector
Additionally, for models with O2 module:
► Second gas pressure hose with quick-release connector
20
Delivery, Transport and Setting Up
3. Delivery, Transport and Setting Up
3.1 Safety regulations
Warning!
You may injure your hands or feet when transporting and
installing the incubator. You should wear protective gloves
and work shoes.
Warning!
Because of the weight of the incubator, you could injure yourself if
you try to lift it on your own.
If possible, only transport the incubator with a fork-lift truck or
manual pallet jack. The incubator may only be moved using a means
of transport by persons who have the required qualification for this
(e.g. fork-lift truck licence). This incubator may not be transported
with a crane.
If the incubator has to be carried, at least two people are required
for models 108 and 153, and at least four people for model 246.
Warning!
The incubator could fall over and seriously injure you. Never tilt the
incubator and transport it only in an upright position.
3.2 Transport
The incubator can be transported in three ways:
► with a fork-lift truck; move the forks of the truck entirely under the incubator► on the manual pallet jack.► by carrying; to do this, at least two people are required for models 108 and 153, and four
people for model 246. Read the weight information detailed on page 18 .
3.3 Delivery
The incubator is delivered in cardboard packaging on a cardboard pallet.
3.3.1 Unpacking
1. Remove cardboard packaging or cut open carefully along an edge.
2. Lift up incubator from pallet and put down on the appliance feet.
3.3.2 Checking for completeness and transport damage
► Check the delivery note to ensure that the delivery is complete.► Check the inside and outside of the incubator for damage.
If you notice deviations from the delivery note, damage or irregularities, do not put the incubator into operation, but inform the haulage company and the manufacturer.
3.3.3 Disposing of packaging material
Dispose of the packaging material (cardboard) in accordance with the effective legal disposal
regulations for cardboard packaging in your country.
21
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