Memmert INCO 108, INCO 108 med, INCO 153, INCO 246 med, INCO 153 med Operating Manual

OPERATING MANUAL

CO2 incubators

INCO 108 INCO 108 med
INCO 153 INCO 153 med
INCO 246 INCO 246 med

anufacturer and

ustomer servic

e

EMMERT GmbH + Co. KG

ostfach 17 2

0

-91107 Schwabac

h

Äußere

Rittersbacherstr . 3

8

-91126 Schwabac

h

German

y

on: [phone] / 925-0
ax: [phone] / 14585

-Mail: [e-mail]

m

nternet: www.memmert.com

ustomer servic

e:

on: [phone]/925-128

and: [phone]/925-126

-Mail: [e-mail]

m

or service enquiries, please always specify the appliance number on the nameplate (see page

15 ).
© 2

0

14 Memmert GmbH + Co. KG

ition 11/2014

Subject to modifications

3

A

bout this manual

About this manua

l

urpose and target grou

p

T

his manual describes the setup, function, operation and maintenance of CO2 incubators

o

f the types INCO and INCOmed with a chamber volume of 108, 153 and 246 litres. In this

manual, the term INCO is used for INCO as well as INCOmed for the purpose of simplification.

istinctions are explicitly pointed out.

T

his manual is intended for use by the trained staff of the operator in charge of operating

and/or maintainin

g

the incubator . If you are asked to work on the incubator, you should read

this manual carefully before startin

g

work on the unit. Familiarise yourself with the safety

re

g

ulations. Only perform the work that is described in this manual. If there is something you

d

on't understand, or certain information is missing, ask your superior or get in touch with the

manufacturer . Do not do anythin

g

without authorisation

.

ntents

T

he INCO incubator is available with different fittings: as a basic model, and with six different

additional modules. The technical fittin

g

s and functional range of the basic model and the

individual additional modules are described from pa

g

e 11 .

f specific equipment features or functions are available only with one of the additional mod-

es, this is pointed out in the relevant sections in this manual.

ue to individual fittings, depictions in this manual may be different from the actual appear-

ance.

ther documents that you must read:

f

or service and repair work (see page

) – a separate service manua

l

torage and Forwardin

g

Th

is instruction manual belongs with the incubator and should always be stored so that those
who work on the incubator have access to it. It is the responsibility of the operator to ensure
that persons who work on or who will work on the incubator are informed as to the wherea­bouts of this instruction manual. We recommend that it is always stored in a protected loca­tion c

l

ose to the incubator . Make sure that the instruction manual is not damaged by heat or

d

amp. If the incubator is sold on or transported and then set up again at a different location,

t

h

is instruction manual must go with it

.

C

ontent

ntent

. Safety regulations 6

1.1 Terms and icons used ..........................................................................................................

1.2 Product safety and dangers ................................................................................................

1.3 Requirements of the operating personnel ..........................................................................

.................................................................................................

1.5 Changes and conversions ....................................................................................................

1.6 Behaviour in case of malfunctions and irregularitie

s

1.7 What to do in case of accidents..........................................................................................

2. Design and Function 1

0

2.1 Design ................................................................................................................................1

0

2.2 Function.............................................................................................................................11

2.3 Optiona

l

extras..................................................................................................................11

2.4 Mat

e

rial.............................................................................................................................1

3

2.5 Electrical equipment

1

3

2.6 Connections.......................................................................................................................1

3

14

2.8 EC Declaration of Conformit

y

...........................................................................................15

2.9 Desi

g

nation (nameplate) ...................................................................................................15

2.1

0

Technical data ....................................................................................................................1

6

2.11 Ambient conditions ...........................................................................................................1

8

2.12 Accessories inclu

ded

..........................................................................................................1

8

. Delivery , Transport and Setting Up 1

9

3

.1 Safety regulations ..............................................................................................................1

9

3.2

Transport ............................................................................................................................1

9

3.3 Delivery

..............................................................................................................................

1

9

3

.4 Setup

.................................................................................................................................2

0

4

. Putting into Operation 2

2

4

.1 Checks

................................................................................................................................

4

.2 Connecting ........................................................................................................................

4

.3 Oxygen calibratio

n

4

5. Operation and control 2

5

5.1 Operating personnel.........................................................................................................

5

5.2 Openin

g the d

oor ............................................................................................................

5

.......................................................................................................

5

5.4 Insertin

g

water tray(s

)

...........................................................................................................

..........................................................................................................

5.7 Basic operation ..................................................................................................................

7

5.8 Sett

ing

parameters............................................................................................................

7

5.9 Operatin

g

modes ...............................................................................................................2

8

..............................................................................................2

8

5.11 During operation ...............................................................................................................34

5.12 En

ding

operation ...............................................................................................................37

6. Warning messages and malfunctions 3

8

6.1 Warning messages ............................................................................................................

38

6.2 System/appliance errors ....................................................................................................

39

......................................................................................................................

42

C

ontent

7. Advanced functions 4

3

7.1 Printer

43

7.2 Basic appliance settings (Setup)

........................................................................................

43

7.3 Temperature monitorin

g

4

5

7.4 Steri

l

isation chipcar

d

49

7.5 User ID card (optionally available as an accessory) ...........................................................

7.6 Calibration .........................................................................................................................

7.7 Communication interfaces ................................................................................................

5

......................................................................................................................

. Maintenance and Servicing 5

9

.1 Cleaning .............................................................................................................................

.2 Regular maintenanc

e

...................................................................................................................

9. Storage and Disposal 6

1

1

9.2 Disposa

l

.............................................................................................................................

1

ndex

6

2

afety regulations

Safety regulations

1.1

Te

rms and icons us

ed

n this manual, certain common terms and icons are used to warn you of dangers or to give

you notes that are important in avoidin

g

injury or damage. Observe and follow these notes

an

d

regulations to avoid accidents and damage. These terms and icons are explained below.

1.1.1 Terms used

"Warning

"

is always used whenever you or somebody else could be injured if you do

ot observe the accompanying safety regulation

.

"Caution

"

is used for information that is important for avoiding damage

.

1.1.2

cons us

ed

Prohibited sign (forbidding an action)

not tilt

applianc

e

o not lift appliance

Warning icons (warning of a danger)

anger of

ele

ctrical shoc

k

xplosive

atmospher

e

anger of frost

-

ite/cold burns

Warning of gas
bottles

heat/hot surface

s

Ga

s

Regulation signs (stipulate an action)

isconnect t

he

mains plu

g

Wear gloves

Wear safety

shoe

s

serve

information in

s

eparate manua

l

Two o

r mor

e

persons require

d

Other icons

nformation on

f

irst ai

d

irst Aid:

nse eyes out

mportant or

seful addition-

al informati

on

7

afety regulations

roduct safety and

anger

s

ncubators of the INCO type are technically well-developed, manufactured using high-quality

materials and tested for many hours in the factory . They contain the latest technolo

g

y and

c

omply with recognised technical safety regulations. But there are still dangers involved, even

w

h

en the appliance is used as intended. These dangers are described below.

W

arning

!

A

fter removing covers, live parts may be exposed. You may receive

an electric shock if you touch these parts. Disconnect the mains plug

efore removing any covers. Any work inside the unit may only be
erformed by qualified electricians.

W

arning

!

W

hen loading the chamber with an unsuitable load, poisonous or
explosive vapours or gases may be produced. This could cause the
chamber to explode, and people could be badly injured or poisoned.
The chamber may only be loaded with materials/test objects which

d

o not form any poisonous or explosive vapours when heated up

see also Chapter 2.7 Intended use on page 14).

W

arning

!

anger of suffocation. In high concentrations, CO2 and N2 can have

a suffocating effect. In normal operation, the incubator gives off

s

mall amounts of

CO

2

and – if equipped with the O2 module – N

2

t

o its environment. You should therefore ensure that the room in

which it is installed is properly ventilated. If a gas bottle is not con-

ected, or is empty, always close the stop valve or pressure reducer

on

the bottle

.

W

arning

!

igh concentrations of CO2 can cause cold burns or frostbite. Avoid

contact w

ith CO

2

gas to the eyes and skin.

W

arning

!

CO

2

gas bottles may burst or explode at high temperature. Do not
use naked flames in the vicinity of the gas bottles. Store gas bottles
at lower than 5

0 °C

in a well-ventilated location. Prevent water from

enetrating, as well as backflow into the gas bottles. It is essential

that you read the safety notes and regulations of the gas suppliers.

2

and N2are not dangerous substances in terms of the German Ordinance on Hazardous
Substances (GefStoffV). Y ou should nevertheless familiarise yourself with the applicable safety
re

gul

ations prior to handling such gas bottles

.

8

afety regulations

1.3

equirements of the

perating personne

l

T

he incubator may only be operated and maintained by persons who are of legal age, and

who have received instructions for the incubator . P ersonnel who are to be trained

,

instructed

o

r who are undergoing general training may only be active on the incubator under the con

-

tinuous supervision of an experienced person.

T

he incubator may only be transported by persons (fork-lift truck, manual pallet jack), who are

trained for this work and who know the correspondin

g

safety regulations

.

epairs may only be performed by qualified electricians. In this case the regulations in the

s

eparate service manual must be observed.

1.4

esponsibility of the owner

The owner o

f the incubator

is responsible for the flawless condition of the incubator and for the incubator being oper-

ated in accordance with its intended use (see pa

g

e

);

is responsible for ensuring that persons who are to operate or service the incubator are

q

ualified to do this, have received instructions about the incubator and are familiar with

t

his operating manual

;

ust know about the applicable regulations, requirements and work protection regula-

t

ions, and train staff acordingly

;

is responsible for ensuring that unauthorised persons have no access to the incubator

;

is responsible for ensuring that the maintenance plan is adhered to and that maintenance

and repair work is properly carried out (see pa

g

e

);

e

nsures, for example through corresponding instructions and inspections, that the incuba

-

t

or and its surroundings are kept clean and tidy;

is responsible for ensuring that personal protective clothing is worn by operating person-

el, e.g. work clothes, safety shoes, protective gloves.

1.5

anges andconversion

s

o independent conversions or alterations may be made to the incubator . No parts may be

added or inserted which have not been approved by the manufacturer .

ndependent conversions or alterations result in the EC declaration of conformity (see page

15 ) losin

g

its validity , and the incubator may no longer be operated.

T

he manufaturer is not liable for any damage, danger or injuries that result from independent

c

onversions or alterations, or from non-observation of the regulations in this manual

.

1.6

haviour in case of malfunctions and irregularitie

s

T

he incubator may only be used when in a flawless condition. If you as the operator notice

irre

g

ularities, malfunctions or damage, immediately put the incubator out of service (see

apter 1.8) and inform your superiors.

Y

ou can find information on eliminating malfunctions from page 38 .

afety regulations

What to do in case of accidents

1.

eep calm. Act resolutely and with consideration. Pay attention to your own

s

afety .

. Switch off the incubator and close the valves on the gas bottle.

3

.

all a doctor.

.

nitiate first aid measures. If available: Call a trained first aid helper .

In case of contact with

CO

to the eyes and skin:

y

inse eyes out with water for at least 15 minutes. With cold burns, rinse with water

fo

r at least 15 minutes. Cover over in a sterile manner . Call a doctor.

Inhaling CO

g

or N

:

igh concentrations can cause suffocation. Symptoms may include a loss of mobility and

c

onsciousness. The victim is not aware of suffocatin

g.

ow concentrations of CO can cause accelerated breathing and headaches

.

A

nyone affected should breathe fresh air , using a breathing device independent of recircu-

latin

g

air . K eep the person warm and calm. Call a doctor. In case of respiratory arrest, use

artificial respiration

.

If gas is escaping:

gpg

eave the room immediately , warn others and ventilate the room. If you re-enter the room,

se an autonomous breathing device (independent of ambient air) if it has not been estab-

ished that the atmosphere is harmless.

1.8 Switching off incubator in an

mer

gency

ush main switch on front side of appliance

. 1 ).

ose the valves on the gas bottle.

Fig. 1 Switch off incubator by

pressing the main switch

g

y

1

0

esign and Functio

n

Design and Functio

n

.1 Des

ign

4

1

2

3

14

13

11
12

9

10

8

7

6

5

18

17

16

15

Fig. 2 Design of INCO incubators

Controller/control panel (see page

27

)

2

Set key

3

Push/turn control

y

4

C

onnections on the rear of the oven

(

see page

)

5

Heating ribs (see page

)

6

Pt100 temperature sensors

7

Humidity sensor

p

8

Glass d

oor

y

9

Chambe

r sea

l

Sliding shelf

1Door sea

l

g

2

Ventilator/inlet opening

Heating ribs

4

Adjustable feet

g

g

Nameplate (beneath door, see page

j

)

Door knob (see page

p

p

25

)

Doo

r

Chip card reader

1

esign and Functio

n

unct

ion

T

he air in the incubator is heated up by an all-round heater with a large surface (

. 3 , No. 1).

Th

e CO and/or

N

g

as is introduced into the working chamber via a sterile filter . Because CO
has a much higher specific weight than air , the gas is let into the working chamber above the
interior fan (2). The turbulence-free interior ventilation (3) ensures a uniform distribution of
t

he g

ases, creating a homogenous atmosphere

.

n the basic version, humidification takes place by means of water trays (4). To avoid uncon­trolled condensation, dosed fresh air is piped into the interior . If the appliance is equipped
with a humidity module, humidification takes place via a hot-air

g

enerator , which allows

water to eva

p

orate at a set rate. The sterile hot air is let into the interior above the fan and is

mixed with the air current (5).

1

1

1

1

1

1

4 4

2

5

3

1

Fig. 3 How INCO incubators wor

k

.3

ptional

xtras

.3.1

asic equipment

lectronic fuzzy-supported PID process controller with pulse width modulation and per-

anent performance adjustments and time-saving self-diagnosis system to quickly locate

e

rrors (see page

39

)

A

ll-round heater with larger surface area, with additional thermal conduction layer (see

. 3

)

apacitive humidity sensor
umidity control (fresh air via sterile filter) ensures that setpoint humidity is quickly

eached and guarantees short recovery times, while avoiding condensation formatio

n

omogenous atmosphere and temperature distribution through encapsulated, turbu-

lence-free ventilation system

TERICard for fully automatic sterilisation process control for hot air sterilisation of appli

-

ance, including sensors and fan rotor (see page 49

)

1

esign and Functio

n

igitalised electronic CO control with automatic zero position, NDIR measuring system

with self-dia

g

nosis system and acoustic error display , air pressure compensatio

n

anguage settings (see page

)

Alph

anumeric text display

ntegrated week time switch with grup function (e.g. each working day) (see page 31

)

etracting push-turn control for simple operation of appliance (see page

)

T

wo separate Pt100 temperature sensors DIN Class A in a 4-wire circuit for control and

onitorin

g

igital monitoring control for overtemperature, undertemperature and automatic

s

etpoint following (ASF) (see page 48

)

echanical temperature limiter (TB protection class 1, see page 45

)

onitoring relay to switch off heater in case of erro

r

ptical alarm displa

y

A

coustic signal messages if temperature or CO limits are crossed, when door is open and

if

g

as bottle is empty (see page 38

)

alibration of temperature, humidity , CO

and O possible on the device without a sepa-

ate PC (see from page

)

.3.2

ptionally availableadditional modules

C

omfort module:

T

wo gas connections with quick release connectors (see page 1

3

)

A

utomatic switch-over of gas bottles

Hygiene module

yg

lectro-polished, seamless laser-welded chamber

C

ommunication modul

e

ogging option of temperature, CO2 and relative humidity via computer/laptop

nternal log memory with 1024 kB as ring memory for all setpoint and actual values, errors

an

d

settings in real time and with date, logging approx. 3 months at 1 minute storage

interval (see pa

g

e

)

arallel printer port (PCL3-compatible) for printing out log data (see page 13 and page 43

)

o

ptionally USB, Ethernet, RS-232 or RS-485 interface (see from page

5

)

CO

2

modu

le

xtended adjustment range from 0 to 20 %

3

-point calibration (5 %, 10 %, 15 % CO

2

)

O

2

modu

le

ontrol of oxygen concentration through the introduction of nitrogen (

N

2

)

; adjustment

ange 1 % to 20 %

O

2

(not in combination with Comfort or Premium module

)

Premium modu

le

ncludes comfort, hygiene, communication and C

O

2

modules

Humidity module

y

A

ctive microprocessor humidification and dehumidification control (40-97 % rh

)

1

3

esign and Functio

n

Materia

l

or the outer housing, MEMMERT uses stainless steel (W .St.No. 1.4016), and for the interior,

s

tainless steel (W .St.No. 1.4301), which stands out through its high stability , optimal hygienic
properties and corrosion-resistance towards many (but not all!) chemical compounds (caution
for example with chlorine compounds). The chamber load for the appliance must be carefully

checked

with respect to chemical compatibility with the materials mentioned.

A

material resistance table can be requested from the manufacturer .

.5

ectrical equipment

perating voltage: See nameplate (page 15 ), 50/60 Hz

urrent consumption: See nameplate (page 15

)

rotection class 1, i.e. operating insulation with safety earth terminal in accordance with
N 6101

0

rotection type IP 20 acc. to EN 60 529

nterference-suppressed acc. to EN 55011 class B

A

ppliance fuse: Fusible link 250 V/15 A quick-blow

T

he temperature controller is protected with a miniature fuse 100 mA (200 mA at 115 V)

or models with a humidity model the CO

controller is protected with a 6.3 A miniature

fuse

.6

nnection

s

3

4

5

6

2

1

H2O

7

N2

In

Fig. 4

Connections on rear of a

ppl

iance

pp

pp

2

Mains lead

3

alternatively Ethernet, RS-232 or RS-485 connection) (details from page

yp

yp

55

)

4

Printer connection (only for models with communication or premium modules) (see page

ypg

4

3

)

5

CO

2

connection to main gas bottle

6

CO

connection to reserve gas bottle (only for models with CO

g

or premium modules)

N

2

connection (quick-release connector, only if equipped with O

2

gy

gy

222

module

)

p

14

esign and Functio

n

.6.1

ctrical connecti

on

bserve the country-specific regulations when making connections (e. g. in Germany DIN VDE

100 with residual current device).

T

his appliance is intended for operation on an electrical power system with a system imped-

anc

e Z

at the point of transfer (service line) of a maximum of 0.292 ohm. The operator must

e

nsure that the incubator is operated only on an electrical power system that meets these

requirements. If necessary , you can ask your local ener

g

y supply company what the system

impe

d

ance is.

.6.2

onnection of external appliances

nly appliances may be connected externally (depending on the model, USB, RS 232, RS 485,

thernet, printer) whose interfaces comply with the requirements for safety extra-low voltage

e.g. PC).

.6.3 Gas connect

ion

Th

e oven can be connected with the supplied compressed air hose via a pressure regulator

with

g

as bottle monitor (DIN 8546) to a CO

compressed gas bottle or directly to a central CO

as supply.

or models with the CO or premium modules, two gas bottles with quick release connectors

c

an be connected. If equipped with the O

module, an N

gas bottle can be connected instead

o

f a second C

O

2

g

as bottle (

. 4 ).

Th

e pre-pressure must not exceed 1.2 bar . A value between 0.8 and 1 bar is considered to be

an i

deal

value.

.6.4 Water connect

ion

or models with a humidity module, the incubator can be connected with the supplied hose

to t

h

e also supplied water supply tank.

.7 Inten

ded

us

e

NCO incubators may only be used for incubating cell cultures or similar . Any other use is

improper , an

d

may result in hazards and damage.

The

incubators are n

ot

e

xplosion-proof (they do not comply with workplace health & safety

re

g

ulation VBG 24). The chambers may only be loaded with materials and substances which

c

annot produce any toxic or explosive vapours at temperature ranges up to 50 ºC, and which

t

h

emselves cannot explode, burst or ignite.

T

he incubators may not be used for drying, vaporising and branding paints or similar materi

-

als, the solvents of which could form an explosive mixture when combined with air . If there is
any doubt as to the composition of materials, they must not be loaded into the incubator . P o­tentially explosive

g

as-air mixtures must not be produced, either in the interior of the chamber

o

r in the direct vicinity of the appliance.

T

he incubator may not be used for sterilisation purposes. It is not a steriliser with respect to

t

he

Law on Medical Devices.

nly gas bottles with a pressure regulator may be connected to the gas connections of the

o

ven. Introducing other gases or materials than C

O

2

or

N

2

is not permitted.

1

5

esign and Functio

n

.7.1

ntended use of the INCOmed

or INCOmed incubators, which are subject to the 93/42/EEC guideline (Council Directive on

the approximation of the laws of the Member States relatin

g

to medical devices), the intended

se is defined as follows:

T

he CO2 incubator INCO med is intended for the creation and maintenance of constant envi­ronmental conditions for application in the field of in vitro fertilisation (IVF), especially for the
incubation of oocytes, spermatozoa and zy

g

otes in special culture dishes for IVF application as

well as for

g

ene expression and the biosynthesis of RNA and proteins.

2.8 EC

claration of

onformit

y

Y

ou can download the EC declaration of conformity of the appliance online:

nglish: http://www.memmert.com/en/service/downloads/ce-statement/
German: http://www.memmert.com/de/service/downloads/e

g

-konformitaetserklaerung/

2.9 Designation (nameplate

)

T

he nameplate (

. 5 ) provides information about the appliance model, manufacturer and

technical data. It is attached to the front of the appliance, on the ri

g

ht beneath the door (see

10 ).

Typ: INC 108 F.-Nr.: 0109.0088
230 V

~

4.4 A 50/60 Hz 1000 W

DIN12880-Kl.3.1 Nenntemp.: 50 °C

1
2

3
4

5

6

10

8

9

7

Fig. 5 Nameplat

e

e designation

2

Operating voltage

yp g

3

Applied standard

pgg

gg

4

Protection type

5

CE conformity

yp

6

Address of manufactur

er

7 Disposal note

8

emperature ran

ge

9

Connection / performance values

pg

g

Factory number

p

1

6

esign and Functio

n

2.10 Technical data

Model

* See Fig. 6 on page 17 .

108 153 246

hamber width A* [mm]

6

0

48040

hamber height B* [mm

]

4804040

hamber depth C* [mm

]

400

A

ppliance width D* [mm] 71

030790

A

ppliance height E* (varies

d

ue to adjustable feet) [mm]

77

8

2

0

3

8

A

ppliance depth F* (includ-

ing door handle) [mm

]

7

90

hamber volume [litres

]

108 15

3

4

6

Weight [kg] 7

0110

erformance [W

]

1

0001500

ax. number of slidin

g

s

helves half size / full size

-/4

-/6

2 x 6/6

ax. load per sliding shelf

k

g]

15 15 15

ax. load per appliance [kg]

40

40

T

emperatur

e

T

emperature recording by means of Pt100 in a 4-wire circuit

Ad

justment range: Normal mode: 20 °C to 50 °C

Sterilisation mode: 160 °C (4 hours) via STERICard

Ad

justment precision: 0.1 °C

ontrol range: from 8 °C above room temperature to 50 °C
Variation (time): max. ±0,1 °C at 37 °C
Variation (spatial): max. ±0.3 °C at 37 °C

umidity

Th

e relative humidity in the chamber is measured by a capac-

itive humidity sensor and displayed digitally in percent.

T

he measurement precision of the humidity sensor is 1 % rh

A

djustment range: 88 to 97 % rh (for models with

umidity module 40 to 97 % rh)

Ad

justment precision: 1 % rh

isplay range: 10 to 98 % r

h

Variation (time): max. ±1 % rh

17

esign and Functio

n

Model

* See Fig. 6 on page 17 .

108 153 246

Th

e CO content is determined through an NDIR measur-

ing system, controlled constantly by a microprocessor an

d

d

isplayed digitally in percent

A

djustment range: 0 to 10 % (for models with CO

or

premium modules 0 to 20 %)

Ad

justment precision: 0.1 %
Variation (time): max. ±0.1 % rh
Variation (spatial): max. ±0.3 %

(only if equipped with O

module

)

The O

content is determined with a long-lasting, mainte-

nance-free zirconium dioxide sensor , constantly controlled by
a microprocessor and displayed digitally in percent

Ad

justment range: 1 to 20 %

Ad

justment precision: 0.1 %
Variation (time): max. ±0.1 %
Variation (spatial): max. ±0.3 %

D
A75 75

38

38

F

C

202

B

76

E

8

12

Fig. 6 Dimensions of INCO incubators

1

8

esign and Functio

n

Ambie

nt conditions

T

he incubator may only be used in enclosed rooms and under the following environmental

cond

itions

:

Amb

ient temperature: 5 ºC to 35 ºC

umidity: max. 80 % not condensing

egree of pollution: 2

A

ltitude of installation max. 3,000 m above sea level

T

he incubator may not be used in areas where there is a risk of explosions. The ambient air

ust not contain any explosive dusts, gases, vapours or gas-air mixtures. The incubator is

ot explosion-proof.
eavy dust production or aggressive vapours in the vicinity of the appliance could lead to

sed

imentation in the chamber interior and as a consequence, could result in short circuits

o

r damage to electrical parts. For this reason, sufficient measures should be taken to
revent large clouds of dust or aggressive vapours from developing.

.1

Accessories inclu

ded

For incubators with basic fittings:

g

T

wo (INCO 108) or three (INCO 153 and 246) sliding shelves

Gas pressure hos

e

Ź Water tray (one for INCO 108 and 153 incubators, two for INCO 246 incubators)
Ź

terilisation chipcar

d

Additionally, for models with humidity module:

y, y

Water supply tank and connection hos

e

Additionally, for models with CO

y,

2

or premium module:

p

econd gas pressure hose with quick-release connecto

r

Additionally, for models with O

y,

2

module:

econd gas pressure hose with quick-release connecto

r

1

9

elivery, Transport and Setting Up

.

elivery, Transport andSetting Up

.1 Safety regulations

W

arning!

You may injure your hands or feet when transporting and

nstalling the incubator. You should wear protective gloves

and work

shoes

.

W

arning

!

ecause of the weight of the incubator, you could injure yourself if

you try to lift it on your own.

f possible, only transport the incubator with a fork-lift truck or

anual pallet jack. The incubator may only be moved using a means

of transport by persons who have the required qualification for this

e.g. fork-lift truck licence). This incubator may not be transported

wi

t

h a crane.

f the incubator has to be carried, at least two people are required

for models 108 and 153, and at least four people for model 246.

W

arning

!

The incubator could fall over and seriously injure you. Never tilt the

ncubator and transport it only in an upright position.

.2 Transpor

t

Th

e incubator can be transported in three ways:

with a fork-lift truck; move the forks of the truck entirely under the incubator

o

n the manual pallet jack.
y carrying; to do this, at least two people are required for models 108 and 153, and four

eople for model 246. Read the weight information detailed on page 16 .

.3

elivery

Th

e incubator is delivered in cardboard packaging on a cardboard pallet.

.3.1 Unpackin

g

1.

emove cardboard packaging or cut open carefully along an edge

.

.

ift up incubator from pallet and put down on the appliance feet.

.3.2

hecking for completeness and transport dama

ge

eck the delivery note to ensure that the delivery is complete.

heck the inside and outside of the incubator for damage.

f you notice deviations from the delivery note, damage or irregularities, do not put the incu-

bator into operation, but inform the haula

g

e company and the manufacturer

.

.3.3

isposing of packaging materia

l

ispose of the packaging material (cardboard) in accordance with the effective legal disposal

re

g

ulations for cardboard packaging in your country

.

2

0

elivery, Transport and Setting Up

.3.4 Storage after delivery

f the incubator is initially to be stored after delivery: Read the storage conditions from page

1 .

.4

etup

T

he incubator can be placed either on the ground or on a table (work surface). When doing

t

h

is, ensure that the appliance is positioned exactly horizontally.

The

installation site must be

level and able to reliably carry the wei

g

ht of the incubator (see page 1

6

)

. Do not place the ap-

pliance on an inflammable surface

.

A

power connection must be available at the installation site in accordance with the connec-

tion data on the nameplate (see pa

g

e 15 ).

The

distance between the wall and the rear of the chamber must be at least 15 cm. The clear-

ance from the ceilin

g

must not be less than 20 cm and the side clearance from the wall not

less than 8 cm (

. 7 ). Sufficient air circulation in the vicinity of the chamber must be guaran

-

teed at all times.

FP



FP



FP



FP



Fig. 7 Minimum clearance from walls and ceiling

1

elivery, Transport and Setting Up

.4.1

ase (accessory

)

T

he incubator can be placed on a base (

. 8 ).

.4.2 Stacking frame (accessory

)

T

wo appliances of the same model size can be placed on top of one another . To do this, foot

ali

g

nment provisions must be attached to the lower oven (

. 9 ):

1.

emove the housing cover from the lower oven.

.

nsert drilling template (supplied with the foot alignments) into the overturned lid.

3

.

ark drilling points and drill with a 4.2 mm diameter drill bit.

.

crew the foot alignment provisions to the top of the lid with the screws and nuts sup-

ied.

5.

ut the covers back on.

Fig. 8

Base

Fig. 9

A

ssembly of the foot alignment

provisions w

h

en two incubators are

p

l

aced on top of one another

utting into Operatio

n

utting into Operation

4

.1

c

ks

ecking the temperature senso

r

specially strong vibrations during transport could

resu

l

t in the temperature sensors being moved

in t

h

eir holders in the working chamber .

heck the temperature sensor for its correct

positionin

g

and if necessary adjust its position

in the holder (

. 10 ).

.1. heck the door and adjust if

cessar

y

ee page 60 .

.

onnectin

g

.

.1

ower supply

C

aution:

bserve the country-specific regula-

t

ions when making connections (e. g. in Germany DIN VDE 0100 with residual current

d

evice / RCD). Observe the connection and power ratings (see nameplate).

T

he incubator is intended for operation on an electrical power system with a system imped-

anc

e Z

at the point of transfer (service line) of a maximum of 0.292 ohms. The operator

must ensure that the incubator is operated only on an electrical power system that meets
these requirements. If necessary , you can ask your local ener

g

y supply company what the

s

ystem impedance is.

onnect power cable (see Fig. 4 on

page

13).

4.2.2

xternal devices

only for models with communication or premium modules

)

nly appliances whose interfaces comply with the requirements for safety extra-low voltge
e.g. PC, laptop, printer) may be connected to the connections on the rear of the incubator
see

. 4on

page

13 ). Which devices may be connected depends on the chosen model /

module variant

(

mmunication interfaces described in detail from page 55 ).

4.2

.3 Water connect

ion

only for models with humidity module

)

or steam creation, use only

d

e

mineralised water (aqua dem) in accordance with

VDE 0510/DIN EN 50272; re

g

ulations must be strictly adhered to (production conductivity

6FP%DWWHU\ZDWHULQDFFRUGDQFHZLWK9'(LVDYDLODEOHLQODUJHUFKHPLVW·V

sh

ops, super markets, hardware stores and in the wholesale trade. The standard VDE 0510/

IN EN 50272 has to be explicitly specified on the label.

therwise, possible calcification in the steam generators, steam piping and peristaltic pumps

c

ould affect the operability of the device.

T

he water used must have a pH value between 5 and 7 and be chlorine-free.

Fig. 10 Temperature sensor

utting into Operatio

n

1.

ill up the supplied water supply tank (canister) with distilled water and place behind/

xt to the incubator.

A

ttach the supplied hose the quick-release connections to the canister and the water sup­ly "H

“ on the rear of the appliance (see

. 4 on

page

13).

.

.4 Gas connect

ion

W

arning

!

anger of suffocation: In high concentrations, CO

and N

can have

a suffocating effect. In normal operation, the incubator gives off

s

mall amounts of CO and – if equipped with the O

module – N

t

o its environment. You should therefore ensure that the room in

which it is installed is properly ventilated.

W

arning

!

igh concentrations of CO

can cause cold burns or frostbite. Avoid

contact w

ith CO

gas to the eyes and skin.

W

arning

!

CO

gas bottles may burst or explode at high temperature. Do not
use naked flames in the vicinity of the gas bottles. Store gas bottles
at lower than 5

0 °C

in a well-ventilated location. Prevent water from

enetrating, as well as backflow into the gas bottles. It is essential

that you read the safety notes and regulations of the gas suppliers.

For incubators with basic fittings:

g

A

ttach the supplied pressure hose to the
as bottle (pressure regulator) and to the

"C

O

2

" connection on the rear of the appli-

ance with two hose clamps (

. 11, see

a

l

so page

).

For incubators with

CO

2

or premium

p

module

:

T

wo gas bottles can be connected by simply

pus

hing th

e supplied pressure hoses onto

t

h

e "C

O

2

In1" and "C

O

2

In2" quick-release

c

onnections on the rear of the appliance

Fig. 12, see also page 13 ).

onnect the main gas bottle to "In1", a re-

s

erve gas bottle can be connected to "In2".

A

ttach the pressure hose to the gas bottles

pressure regulator) with hose clamps.

CO

2

„CO2 In“

Gas connection for incubators with basic

f

ittin

gs

CO2CO

2

„CO2 In1“
„CO

2

In2“

Fig. 12
Gas conn

e

ction for incubators with

CO

o

r Premium modul

es

4

utting into Operatio

n

For incubators with

O

2

module:

A

ttach the su

pplied

CO

pressure hose

t

o the C

O

2

as bottle (pressure reducer)

an

d

to the “CO2 In” connection on the
ear of the incubator with a hose clamp
Fig. 13, see also page 1

3

)

.

A

ttach (push on) the supplied N2 pres-

sure hose to the

N

gas bottle (pressure

educer) and to the “N2 In” connection

o

n the rear of the incubator with a hose

cl

amp

.

4.3

xygen calibratio

n

only for models with O

module

)

erform an Ocalibration before putting

into operation (see pa

g

e 54 ).

CO2N

2

„CO2 In“

„N

2

In“

Fig. 13
Gas conn

e

ction for incubators with O mod-

ul

e

Operation and control

.

peration and

ntr

ol

5.1

perating personne

l

T

he incubator may only be operated by persons who are of legal age and have received in-

s

tructions for the incubator . P ersonnel who are to be trained, instructed or who are undergo-

in

g g

eneral training may only be active on the incubator under the continuous supervision of

an experience

d

person.

5.2 Opening the door

T

o open the door , turn handle to the right ( Fig. 14 ).

T

o close, turn door handle to the left.

W

h

en the door is opened, the CO

supply is automati-

c

ally interrupted. If the heated outer door is left open for

any len

g

th of time, condensation may form on the glass

doo

r.

5.3

oading the incubato

r

W

arning

!

Wh

en loading the chamber with an

unsu

itable load

, poisonous or explosive

v

apours or gases may be produced. This

could cause the chamber to explode, and people could be badly in-

j

ured or poisoned. The chamber may only be loaded with materials/

test objects which do not form any toxic or explosive vapours when

eated up, and which cannot ignite. If there is any doubt as to the

composition of materials, they must not be loaded into the incuba-

t

or.

C

aution:

ck the chamber load for chemical compatibility with the materials of the incubator
see page 13), since considerable damage could otherwise occur to the chamber load,

th

e appliance or the surroundings

.

The

incubators are notexplosion-proof (they do not comply with workplace health & safety

re

g

ulation VBG 24) and are therefore not suitable for drying, vaporising and branding paints

o

r similar materials, the solvents of which could form an explosive mixture when combined

with air . P otentially explosive

g

as-air mixtures must not be produced, either in the interior of

the chamber or in the direct vicinity of the appliance.

eavy dust production or aggressive vapours in the chamber or in the vicinity of the appli-

ance cou

ld lead

to sedimentation in the chamber interior and as a consequence, could result

in short circuits or dama

g

e to electrical parts. For this reason, sufficient measures should be

taken to prevent lar

g

e clouds of dust or aggressive vapours from developing.

Th

e chamber must not be loaded too tightly , so that proper air circulation in the working

c

hamber is guaranteed. Do not place any of the chamber load on the floor , touching the side

walls or ri

g

ht below the ceiling (heating ribs) of the working chamber . To guarantee an opti-

mal air circulation, push in the slidin

g

shelves so that the gaps between the door , sliding shelf

and rear wall of the chamber are rou

g

hly the same size.

open

close

Fig. 14 Opening and
c

l

osing the door

Operation and contro

l

5.4

nsertingwater tray(s

)

for appliances with basic fittings

)

ill the water tray with distilled water and push into the lowest slot (

. 15 ).

Fig. 15 Inserting water tray(s

)

INCO
model

Number

of

water

trays

Filling

level in

cm for

each tray

approx.

Amount
of water
in ltr . for

each tray

approx.

1 1.5 to 2.5 1 to 1.5

15

3

1 1.5 to 2.5 1 to 1.5

4

6

1.5 to 2.5 1 to 1.5

5.5 Connect

as supp

ly

1.

heck that the gas bottle(s) is properly connected (see also page 13 ).

.

pen valve(s).

5.6 Switch on applianc

e

The incubator is switched on and off by pressing the main switch/

push-turn control on the

front of the appliance.

witching on:

ress the main switch so that it comes out of the appliance (

. 16 ).

witching off: press the main switch so that it retracts back into the appliance ( Fig. 17 ).

Fig. 16 Switching on incubator

Fig. 17 Switching off incubator

7

O

peration and control

5.7 Basic operatio

n

T

he desired parameters are entered on the operating panel of the controller on the front of

the appliance (

. 18 ). Basic settings, as well as those for time and printing, can also be made

ere. In addition, programmed and current parameters are displayed, as well as warning mes-

sages:

PRINT

SETUP

loop

t3

t4

t2

t1

on
off

Mo

Tu

We

Th

Fr

Sa Su

3

4

2

1

STERI DEFRO

°C

°C

rh

mb

%

CO

mb

2

IN 1

IN 2

OUT

IN 1

IN 2

OUT

MIN

AUTO

MAX

123465 7 8 109 11 12

13141516

Fig. 18 Operating panel

ime displa

y

Display appliance locked with user-ID

py

py

card (see page

pypp

p

50

)

3

Display appliance is heating up

pg

pg

Sterilisation mode (see page

pypp g

ypp

49

)

5

6

Alarm display

p

p

7

Monitoring temperature display

py

(

see page

45

)

8

Ho

rn

9

Humidity display

g

g

1

Gas bottle 1 active

ypy

y

2 Gas bottle 2 activ

e

CO

displa

y

4

Display appliance is humidifying

py

Operating mode display (see page

pypp yg

28

)

ext display/O

pg

g

display (O

py

p

display only if

pg

pg

equipped with O

py

y

2

2

module)

py

2

A

ll operating functions are selected by turning the push-turn control to the

left or ri

g

ht...

..and adjusted by turning this with the SET key held down.

5.8 Setting

arameters

n general, all setting actions on the operating panel described on the following pages are

ma

d

e in the same way:

1.

Y

ou select the desired parameter with the push-turn control (menu item, e. g.

temperature); then all other parameters

g

o dark and the selected one flashes.

. With the SET key held down, set the desired value (e. g. 37.0

C) with the

pus

h

-turn contro

l.

3

.

elease the SET key , and the set value is saved. The display briefly shows the

s

et value, flashing. The current temperature is displayed and the incubator

egins to heat up to the set temperature.

Settings for other parameters are made in the same way .

set

set

set

set

set

2

8

Operation and control

T

he control returns automatically to the main menu if the push-turn key or set key is

not operated for approx. 30 seconds.

Setting the temperature (Quick adjustment):

gp(Qj)

1.

old down the SET key and set the desired temperature setpoint with the push-turn

co

ntrol.

.

elease the SET key

T

he appliance flashes briefly , showing the temperature setpoint. Then the current temperature

appears on t

he d

isplay and the controller begins to move to the set temperature.

5.9 Operating modes

NCO incubators can be operated in three ways (

. 19 ).

ormal mode: The incubator runs in permanent operation at the temperature, humidity

an

d CO

values set on the operating panel. Operation in this mode is described from page

2

9

Week time switch: The incubator runs at the set values only at certain times. Operation in

t

his mode is described from page 31 .

nterface mode with PC/laptop (for models with communication or premium modules, see

f

rom page

5 )

n addition, basic appliance settings can be made (SETUP, see page 43 ) and printouts can be

made if the appliance is equipped with the communication or premium module (PRINT, see

43 ).

PRINT

SETUP

rma

l

o

peratio

n

see Page 29

)

W

eek

tim

e

s

witc

h

see Page 31

)

rint

er

see Page 43

)

asic appliance

s

ettings

see Page 43

)

Fig. 19 Operating mode

s

5.10 Setting the operating mode

1.

old SET key down for approx. three seconds, the selected operating mode

then be

g

ins to flash.

. Select the desired operating mode (normal mode, week time switch, pro-

ramming mode, printer or basic appliance settings/setup by turning contro

l

with SET key held down.

3

.

elease the SET key , and the selected operating mode is saved.

se

t

set

set

2

9

O

peration and control

5.10.1

rmal m

ode

T

he appliance runs in this operating mode in permanent operation. The desired setpoints for

o

perating the chamber can be selected. The settings have an immediate effect on the func-

tions of the appliance.

oad incubator (see page

5 ).

.

witch on appliance. To do this, press the push-turn control on the operating panel so that

it comes out of the appliance (see

. 16 on

page

).

3

.

elect the normal operating mode

with the push-turn control:

PRINT

SETUP

. As described above, set the individual parameters with the push-turn control and the set

ey:

Temperature setpoint

pp

Ad

justment range:

to

0°

°C

Temperature monitoring

pg

Ad

justment range:

IN MAX AUT

O

see also page 45

)

°C

MIN

AUTO

MAX

yp

Ad

justment range:

r incubators with basic

f

ittings: 88 to 97 %r

h

or models with humidity

ule: 40 to 97 %r

h

rh

%

igh levels of air humidity in the interior can only be achieved without condensation if the

interior is t

h

orou

ghly h

eated. Please set the humidity only when the temperature in the

interior has been stable for at least 3

0

minutes.

2

setpoint

p

Ad

justment range: 0 bis

0 %

or models with CO2 or premium

mo

dul

es 0 to 20 0 to 20 %

)

CO

2

2

setpoint

p

2

mod-

le

)

Ad

justment range:

Off

to 20

%

t2

t1

on
off

Mo

Tu

We

Th

Fr

Sa Su

o2=3.4%

30

Operation and contro

l

5.10.2 Settings example normal mo

de

With a 5% COcontent, a 3% oxygen content and a humidity of 96%, the appliance should
heat up to 37°C. The monitorin

g

function should respond at 38.5 °C (

. 20 ).

30

40

20
10

50

60

80

40
20

100

Temperature in °C

Humidity rh in %

Time Time Time

CO

2

in %

5

10

15

20

25

Time

O

2

in %

5

10

15

20

25

Monitoring temperature

Fig. 20 Example for normal mode

1.

etting the normal operating mode:

old SET key down for approx. 3 seconds, the current

o

perating mode then begins to flash.

S

ele

ct the

operating mode with the push-turn con-

trol, while the SET key is held down. After you let go of
the SET key , the control is in the normal operating mode.

.

etting the temperature setpoint:

old down the SET key and set the desired temperature

s

etpoint of 37.0°C with the push-turn control.

elease the SET key , the appliance will briefly flash, show-

ing the temperature setpoint. Then the current tempera-

ture

appears on the display and the controller begins to move
to the set temperature of 37.0 °C.

eating up is indicated by the icon

.

°C

3

.

etting the monitoring temperature:

T

urn the push-turn control to the right until the monitor-

ing temperature and the MIN or MAX icon flashes. Hold

d

own the SET key and with the push-turn control, set
the overtemperature limit to 38.5 °C and the undertem­perature limit to 36.0°C. Turn the push-turn control to
the right until the monitoring temperature and the AUTO
icons flash. Hold down the SET key and set to on with the
push-turn contro

l.

T

he tolerance band is set in the SETUP menu (see

44).

°C

MIN

AUTO

MAX

PRINT

SETUP

31

O

peration and control

.

etting the humidity setpoint:

igh levels of air humidity in the interior can only be

achieved without condensation if the interior is

th

orou

ghly h

eated. Please set the humidity only when

t

he temperature in the interior has been stable for at

east 30 minutes.

T

urn the push-turn control to the right until the humid-

ity display flashes. Hold down the SET key and set the

d

esired humidity setpoint of . %rh with the push-turn

c

ontrol. After releasing the SET key , the humidity setpoint
briefly flashes. The current humidity value appears on
the display and the controller begins to move to the set
value.

T

he humidification process is indicated by the icon

only for models with humidity module).

rh

%

5.

Setting the

g

setpoint

p

T

urn the push-turn control to the right until the CO

d

is-

play flashes. Hold down the SET key and set the desired

2

setpoint of 5.0 % with the push-turn control. The

appliance flashes briefly , showing the C

O

2

setpoint. The

c

urrent C

O

2

actual value appears on the display and the

c

ontroller begins to move to the set CO2 setpoint.

ayed by the

IN 1

or

IN 2

icon.

CO

2

.

Adjust the

j

2

setpoint

p

only for models with O2 module

)

T

urn the push-turn control to the left until the O2 display

f

lashes. Hold down the set-key and set the desired O

2

s

etpoint of 3.0 % with the push-turn control. Release

the set-key . The appliance flashes briefly, showing the O

s

etpoint. Then, the current O

actual value appears on

the display and the controller begins to move to the set

value.

on

off

Mo

Tu

We

Th

Fr

Sa Su

o2=3.0%

The incubator is now running in permanent operation with the set values.

5.10.3 Week time switch

n this operating mode, the appliance switches on and

o

ff automatically at the times programmed

.

uring the OFF phase of the week time switch, the

appliance is in standby mode. The heatin

g

and cooling functions, along with the CO2 an

d

humidity supply are switched off here and the controller display shows the time, dimmed.

T

he sequence of the week time switch repeats itself each week.

n total, a maximum of 9 time blocks can be programmed, consisting of the switching on and

s

witching off times.

PRINT

SETUP

32

Operation and control

Weekday

y

Ad

justment range: Monday to Sunday

Mo

Tu

We

Th

Fr

Sa

Su

yg p

Ad

justment range: Working days Mo-F

r

W

eekend

Sat-Sun

Mo

Tu

We

Th

Fr

Sa

Su

Mo

Tu

We

Th

Fr

Sa

Su

switch on time: ---

Appl

iance not switched on on this day

on

off

Switch on time (on

)

()

Ad

justment range:

0

0:00 to 23:59 hours

on

off

h

Switch off time (off

)

()

ne minute beyond the switch on time up to

24:0

0

on

off

h

sel

ected as in the normal operating mode.

f no settings (temperature setpoint etc.) are made for the ON phase, the controller takes

o

ver the values from the normal operating mode.

or reasons of safety , you should always check that only one switch one time is programmed
in the desired time blocks and days. By turnin

g

further to the right, parameters (temperature

s

etpoint etc.) can be selected as in the normal operating mode.

f the controller is in standby mode or the week time switch is in the ON phase, the tempera-

ture setpoint can be directly accessed by briefly pressin

g

the SET key . By turning the control to

t

h

e right, you are returned to temperature monitoring, humidity and CO2 setting. By turning

to the left, you come back to the settin

g

s for the individual time blocks.

33

O

peration and control

5.10.4

ettings example week time switc

h

rom Mo-Fr (workdays group), the appliance should switch on at 9.30 and switch off at

19.00. In addition it should work on Saturday from 10.00 to 14.00 (

. 21 ).

Mo

Sa

Fr

Th

We

Tu

Su

9:30h - 19:00h

24:00h

12:00h

01:00h

9:30h - 19:00h

9:30h - 19:00h

9:30h - 19:00h

9:30h - 19:00h

10:0h -

14:00h

Fig. 21 Operation with week time switch (example)

1.

etting the week time switch operating mode

old the SET key down for approx. 3 seconds, the current

o

perating mode then begins to flash. Select the week

time switc

h

operating mode with the push-turn control,

w

hil

e the SET key is held down.

elease the SET key , the control is now in the week time

s

witch operating mode.

PRINT

SETUP

.

witch on Mo-Fr at 09:3

0

T

urning the push-turn control to the left, select the

Mo-Fr on

"

icons (group working days)

.

old down the SET key and set the desired switch-on

time wit

h th

e push-turn switch to 9:

30

on

off

Mo

Tu

We

Th

Fr

Sa Su

3

.

witch off Mo-Fr at 19:

00

S

ele

ct "Mo-Fr

off

" (group working days) with the push-

turn c

o

ntr

ol.

old down the SET key and set the desired switch-off

time wit

h th

e push-turn switch to 19:00

.

on

off

Mo

Tu

We

Th

Fr

Sa Su

3

4

Operation and control

.

witch on Sa at 10:

00

With the push-turn control, select "

Sat on

".

old down the SET key and set the desired switch-on

time wit

h th

e push-turn switch to 10:00

.

Mo

Tu

We

Th

Fr

Su

off

Sa

h

on

5.

witch off Sa at 14:

00

With the push-turn control, select "Sat off

old down the SET key and set the desired switch-off

time wit

h th

e push-turn switch to 14:00

.

Mo

Tu

We

Th

Fr

Su

on

Sa

h

off

5.10.5 Operation with

C/laptop (optional

)

f equipped with the communication or premium module, the incubator can optionally be

sed, controlled and programmed with a PC/laptop. It has corresponding communication

interfaces for this purpose (see pa

g

e 13 and page

5 ).

peration is described in a separate manual. It is delivered with the incubator for

the rele

vant m

odel

s.

.11

uring operatio

n

egularly check the water level. If necessary , add distilled water.

Warning messages during operation: See Page 38 .

CO

2

mod

e

n the heating up phase, the CO2 controller is initially deactivated. The CO2 intake is inter­rupted during this period. About 5 minutes after the setpoint temperature has been reached,
t

h

e C

O

2

control begins measuring and C

O

2

gas is let in to the chamber via a sterile filter (the

valve on the

g

as bottle must be open). To ensure a homogenous distribution of the CO2 gas in

the interior , the

g

as is piped in above the chamber fan. The setpoint can be adjusted in 0.1 %

s

teps from 0 to 10% (for models with CO2 or premium modules 0 to 20 %)

A

fter a sterilisation procedure, and in cycles every 24 hours, an automatic zero balance

adjustment is carried out. This automatic zero balance adjustment is completed after a

fe

w minutes.

Displays in CO

py

2

mode:

HEAT UP

H

EAT UP is shown during the heating up phase of

th

e C

O

2

sensor . In the C

O

2

display , CO2 is shown.

A

UTOZERO - DO NOT OPEN DOO

R

is displayed during the

zero balance adjustment.

35

O

peration and control

CO

2

IN 1

IN 2

OUT

A

fter the setpoint temperature has been reached,

th

e C

O

concentration is displayed in %, depending

o

n the setting.

IN 1

indicates that gas bottle 1

s active.

s displayed if the CO

concentration exc

eed

s the

d

efined setpoint by at least 1 % for more than 3

minutes. If the concentration is hi

g

her , the CO

d

isplay and the

icon flash.

n this case you should open the door for 30 sec.

and wait to see if the controller steadily adjusts

t

o the setpoint. If the error occurs again, contact

ustomer Servic

e.

T

his monitoring function only starts to work

o

nce the CO setpoint has been reached.

C

O2 emp

t

is displayed if gas bottle 1 and/or 2 is/are

e

mpty . In this case, you should connect new gas

bottles (see pa

g

e

).

Th

e pressure in the gas bottles is a constant

a

pprox. 57 bar at 20 °C ambient temperature. It

is not

p

ossible to determine how full the bottle

is t

h

rough the pressure, since the pressure only

d

rops immediately before the bottle is com-

p

l

etely empty .

Th

e C

O

2

supply is automatically interrupted when

th

e outer doors are opened.

door open

is indicated

in t

h

e text display .

on
off

2

empty

2

supply is interrupted. In this

c

ase, check if the N2 bottle is correctly connected

and if the valve is open.

f that does not solve the problem, you should con-

nect a new

g

as bottle (see page

).

Automatic switch-over of

CO

2

gas bottles (only for models with comfort or premium

g(y p

modules)

)

T

he automatic switch-over of gas bottles guarantees an uninterrupted supply with CO2 gas

w

h

en two independent supply systems are connected.

Gas bottle 1 is always the main supply bottle.
Gas bottle 2 is always the reserve bottle.

peration is only possible with one bottle. In this case it must be connected to IN1 (see also

. 4 on

page

).

36

Operation and control

T

o be on the safe side, a freshly filled CO

gas bottle should always be used. So if the gas in

ottle 1 is used up, you connect the opened bottle to IN1 and the newly filled bottle as a

serve bottle to IN2.

T

he hose connection system used by Memmert shuts off automatically if a connection hose is

pulled off. You should still always close the stop valve on the

g

as bottle if a bottle is empty or

n

o

t connected.

IN 1

lights up when gas bottle 1 is active. If gas bottle 1 is empty ,

there

is an automatic switch-over to the reserve bottle.

IN 2

lights up after the switch-over to gas bottle 2 (reserve bottle).
Switc

hing

over to the reserve bottle is marked by a short re-

peated acoustic si

g

nal (about 3 seconds long) (the default after

s

witching on is gas bottle 1).

n the following cases, a switch-over is made from reserve bottle 2 back to the main supply

ttle 1:

if the reserve bottle is empty

e

ach time after the appliance is switched on

after every change of the CO

setpoint

Humidity limit control

y

A

humidity limit control prevents the formation of condensation water in the chamber and

at t

h

e same time ensures that the setpoint humidity is quickly reached, with short recovery

t

ime

s.

T

he maximum achievable humidity can be adjusted in the standard model from 88 to 97% rh.

Active humidity control (only for models with humidity module)

y(y y)

Th

e active humidity control guarantees that setpoint humidity is quickly reached, without the

se of water trays.

n the heating up phase, the humidity control is initially deactivated. Approx. 5 minutes after
the setpoint temperature is reached, the humidification and dehumidification control starts
workin

g

. The setpoint can be set from 40 to 97 % rh. The humidity setpoint can also be ad-

j

usted during the transient state. For humidification, water is let into the chamber via a dosing
pump. To avoid the formation of

g

erms, the steam is first heated to approx. 140 °C. Dehumid-

ifcation takes place throu

g

h the supply of dry air via a sterile filter .

f no humidity is required in the chamber , the humidity control can be deactivated by

s

etting it to

OFF

.

3

7

O

peration and control

5.12

nding operatio

n

1.

witch off appliance. To do this, press the

ain switch on the operating panel so

th

at it clicks into place in the appliance

see

. 22).

.

lose the valve(s) of the gas bottle(s).

3

.

pen the door (see page

5 ).

.

move the chamber load.

5.

or appliances with the basic model:

emove and empty the water trays. Fill

water trays an

d

insert them only when

th

e appliance is next used.

or incubators with a humidity module,

e

mpty the water tanks if the incubator is

ot used for several days.

Fig. 22

S

witching off incubator

38

W

arning messages and malfunctions

. Warning messages andmalfunction

s

.1 Warningmess

ages

A

n intermittent tone is also set off by the warning messages. This can be temporarily

s

witched off by pressing the SET key.

DOOR

OPENif the door of

a

ppliance is opened for

onger than 3 minutes.

emedy: Close the door

Error in the temperature control system (see also Chapter “

py(p

pg

”

on page

pg

45

):

)

Tb active

- temperature

limiter triggered if the

t

emperature limiter

respon

d

s

emedy: Switch off the appliance

and l

eave to coo

l down. If the

e

rror occurs again after switch

-

ing the
appliance back on: Switch off

th

e appliance and Contact the

c

ustomer servic

e

h

i-alarm- overtempera-

ture al

arm limit max ex

-

c

eeded if overtemperature

rotection responds

emedy: Check the setting of

th

e MAX temperature monitor-

in

g

(see Chapter “ Temperature

mon

i

toring ” on page 45

- undertem-

erature alarm limit min

c

rossed if undertempera-

ture

protection responds

emedy: Check the setting of

t

he MIN temperature monitor-

in

g

(see Chapter “ Temperature

mon

i

toring ” on page 45

a

sf alarm- temperature

o

utside tolerance band if

a

utomatic monitorin

g

f

unction responds

emedy: Check the setting of the

A

SF temperature monitoring (see

utomatic temperature

monitor (ASF) ” on page 48 .

Error in humidity system (only for appliances with humidity module)

yy ( y pp y )

if the water supply is

defe

ctiv

e

emedy: Check that the water

s

upply hose is properly con-

necte

d

. Fill the water supply tank

with distilled water if it is empty .

39

W

arning messages and malfunctions

f humidity exceeds the

preset setpoint for lon

g

er

t

han 30 minutes.

emedy: Open door for 30 sec.

and wait t

o see

if the controller

s

teadily adjusts to the setpoint.

f the error occurs again, Contact

the

customer service.

Error in

CO

system:

y

if the CO supply is defec-

tive

emedy: Set CO

setpoint value

t

o 0, check stop valve of gas
bottle(s) and ensure that they
are properly connected; if

g

as

bottle(s) empty , chan

ge g

as bot-

tles,

set CO

setpoint to desired

va

lue

f the COconcentra

-

tion excee

ds the defined

s

etpoint by at least 1 % for

m

ore th

an 3 minutes.

emedy: Open door for 30 sec.

and wait t

o see

if the controller

s

teadily adjusts to the setpoint.

f the error occurs again, Contact

the

customer service.

Error in

O

2

system:

y

on
off

Mo

Tu

W

e

Th

Fr

Sa Su

2

empty

2

supply is defective

emedy: check if the N2 bottle
is correctly connected and if the
valve is open. If that does not

sol

ve the problem, you should

c

onnect a new gas bottle (see

).

.2 System/appliance

rror

s

W

arning

!

A

fter removing covers, live parts may be exposed. You may receive
an electric shock if you touch these parts. Malfunctions requiring in­tervention inside the appliance may only be rectified by electricians.

Y

ou must read the separate service manual for the INCO incubator

for

this

.

o not try and rectify the error yourself, but contact an authorised customer service point for

EMMERT appliances or Contact the customer services department directly of the company
EMMERT (see page

).

n case of enquiries, please always specify the model and appliance number on the nameplate
see page

5 ).

40

W

arning messages and malfunctions

Error P ossible causes Remedy

d

isplay although the

incubator is switc

hed

on.

Power supply
interrupte

d

heck power supply and fuse/safety

s

witch.

Appliance fuse or
miniature fuse or
controller fault

y

ntact the customer service and rea

d

the se

rvice manua

l.

Appliance error
Mainboard fault

y

Appl

iance cannot be

o

perate

d

Incubator loc

ked

with

ser-ID car

d

Unlock incubator with user-ID card (see

).

Push/turn contro

l

fault

y

ntact the customer service and rea

d

the se

rvice manua

l.

o CO display shown

in the CO modu

le

Switched mode power

s

upply SP 200 faulty

ntact the customer service and rea

d

the se

rvice manua

l.

icon flashes ...

Temp

erature fuse

(TWW , ASF) has re-

s

ponde

d

ncrease temperature difference

etween monitoring and working

t

emperature (see page 45 ).

eplace Pt100 temperature sensor

o

f monitoring controller if necessar

y

see Service manual

)

... and rh empt

y

Water supply tank
empty

Set humidity setpoint to OFF, fill up

d

istilled water , then reset humidity

s

etpoint back to desired value.

... and rh empt

y

Humidity setpoint

exceeded

pen door for 30 sec. and wait to see if

sub

sequently the controller steadily ad-

j

usts to the setpoint. If the error occurs

again, Contact the customer service

.

... and

co2 over

2

setpoint is ex-

c

eeded

pen door for 30 sec. and wait to see if

sub

sequently the controller steadily ad-

j

usts to the setpoint. If the error occurs

again, Contact the customer service

.

... anderror Autoze

ro

A

utozero pump

f

aulty

2

contr

olle

r

f

ault

y

ntact the customer service and rea

d

the se

rvice manua

l.

rror display (E..) in

d

isplay

Appliance/system error

ntact the customer service and rea

d

the se

rvice manua

l.

1

W

arning messages and malfunctions

Error P ossible causes Remedy

rror message CON

F

is displayed for only 10

s

ec. after switching on

)

hecksum error (error
when saving setpoint
values

)

T

he error can be rectified just by the

c

ontroller after a setpoint parameter

has again been saved. If the error

should

continue occurring, or cannot be
rectified: Contact the customer service
and replace controller (see service
manual

)

hamber fan without

functi

on

Miniature fuse or
mains adapter fault

y

ntact the customer service and rea

d

the se

rvice manua

l.

eating icon

not on

Amb

ient tempera-

t

ure too hi

gh

Setp the appliance up in a cooler room,
minimum setpoint temperature = am-

ient temp. + 8 °C

T

emperature in

appliance higher

th

an set setpoint

t

emperatur

e

Wait until appliance has cooled down.

icon is permanently

it up

T

emperature fuse has

responde

d

Switch off appliance and leave to

cool down

eck temperature limiter

Th

e appliance is only operational again

after it has cooled down and the error

as

been el

iminated.

CAL

O

2

ERRO

R

Fault in oxygen calibra

-

t

ion

pen the door for one minute and

s

tart calibration again. If the message

appears gain: Contact the customer

service

.

ERROR O

2

Sensor

xygen sensor fault

y

ntact the customer servic

e

W

arning messages and malfunctions

.3

wer failur

e

n case of a power failure, the incubator operates as follows:

In normal and week time switch operating modes

pg

A

fter the power supply has been restored, operation is continued with the parameters set.

T

he time and the duration of the power failure is documented in the

og memory

.

For remote operation

p

f there is a power failure in remote operation, the appliance starts in the normal operating

mode for safety reasons and all setpoint values are chan

g

ed to safe default values (see table).

T

he programme can only be continued from the computer . The time and duration of the

power failure are documented in the lo

g

memory .

Parameters Default value

T

emperatur

e

20 °C

off

(only for models with O

module

)

OFF

43

A

dvanced function

s

Advanced function

s

7.1 Printer

only for models with communication or premium modules

)

ncubators with a communication or premium module are equipped with a parallel printer

port, as used in computers. Standard PCL3 compatible inkjet printers with a parallel port (e.

g

.

P DeskJet 5550 or HP DeskJet 9xx) can be connected to the printer interface on the rear of

the appliance (see pa

g

e ).

ake sure that a shi

elded

interface cable is used. The shielding must be connected to the plug

casing.

The co

ntr

oller h

as an internallog memory (see page

). The log data can be printed out in

t

h

is mode via the connected printer .

f a colour printer is connected, the various graphs are printed out in colour .

or a printout, the GLP header is also printed automatically , containing the following details:

ate of printout

eriod of lo

g

onsecutive page numbers

erial numbers and appliance nam

e

Running the printing function:

gp g

Select the PRINT operating mode with the
pus

h

-turn control, as described on page 28 . By

turnin

g th

e push-turn control and holding down

the SET key , the followin

g

parameters can be selected and altered one after another , as

d

escribed in Chapter “

asic operation “ on page

7 :

uerying the date of the first print page

uerying the date of the last print page

eaving the print menu and returning to the main menu

Basic appliance settings (Setup

)

Setting options may vary depending on the appliance model)
n this operating mode, the basic settings for the

app

l

iance can be made. The time, date, day and

year are set here, alon

g

with the settings for the

orn, the address allocation, the monitorin

g

nits and the calibration.

PRINT

SETUP

PRINT SETUP

4

A

dvanced function

s

y turning the push-turn control and holding down the SET key , the following parameters can

be selected and altered one after another , as described in Chapter “

asic operation “ on page

7 :

lock time in 24 hr . format

onversion to summer time is not automatic,
ut must be done manually.

at

e

Th

e controller contains a calendar which automatica

lly

accounts for the different lengths of months, and for leap
y

ea

rs.

Weekday

Tu

Year

A

djustment range: from 2000 to 210

0

Acoustic signal at programme end

gpg

ENDSOUN

D

Setting:

OFF

or

ON

Acoustic Signal for alarm, e.g. over/undertemperature

g,g/p

ALARM s

o

Setting:

OFF

or

ON

mmunication addres

s

A

djustment range: 0 to 15 (see Chapter

mmunication interfaces “ on page

5

)

A

djustment range: 0.5 bis 5°C (see page 48

)

Language

gg

Setting: GERMAN, English, franCAIS, ESPANOL and italIAN

O

CAL 1-3 rh20 RH9

0

)

customer-side calibra

-

tion of temperature and humidity (see Chapter “

alibration “

on page

5

0

)

45

Advanced function

s

yg

(Cal

0

)

only for models with O

module, see page

4

)

on

off

CAL o2 off

p

Save all settings and leave the SETUP mode.

The

realtime clock, which is set in the SETUP, contains the date and clock time. It is used

for lo

gging

purposes in accordance with GLP. Date and clock time are specified on the lo

g

printout. On graphical printouts, the time axis is labeled with the realtime. The clock is battery­buffered and independent of the mains connection. The inte

g

rated Lithium battery of the

type CR 2032 has a lifetime of approx. 10 years.

7.3 Temperature monitorin

g

The

monitoring temperature is measured via a separate Pt100 temperature sensor in the

chamb

er interior . The monitoring unit is used to protect the chamber load and as a protection

for the appliance and surroundin

gs.

T

he appliance has a double overtemperature fuse (mechanical/electronic) in accordance with

IN 12880.

°C

MIN

AUTO

MAX

1

2

3

1

6
5

4

Fig. 23

Visual alarm icon

p

Lit up: TB alarm

Flashing: TWW alarm, ASF alarm

p

p

2

Undertemperature limit

g

g

3

Automatic temperature monitor (ASF, see page

48

)

4

Overtemperature protection (TWW, TWB, see page

46 )5

Response temperature

6

Acoustic alarm icon

pp

7.3.1 Mechanical temperature monitoring:temperature limiter (TB)

T

he incubator is equipped with a mechanical temperature limiter (TB) protection class 1 in ac-

cord

ance with DIN 12880.

f the electronic monitoring unit should fail duiring operation, and the factory preset maxi-

tive measure, switches off the heating permanently . As a warning, the icon

lights up.

46

A

dvanced function

s

Error rectification after the TB has been triggered:

gg

witch off appliance and leave to cool down

ontact the customer service and have the error rectified (e.g. replace temperature sen-

s

or).

T

he appliance is only operational again after it has cooled down and the error has been elimi-

nat

ed

.

7.3.2 Electronictemperature monitoring

(

T

WW

)

T

emperature monitoring can be adjusted independently of the operating modes.

Th

e manually set monitoring temperature

MIN

and

MAX

the overtemperature control is
monitored by an adjustable over/undertemperature controller (TWW) protection class 3.3 acc.
to DIN 1288

0

.

f the manually set monitoring temperature

MAX

is exceeded, the TWW takes over tempera-

ture control and be

g

ins to regulate the monitoring temperature (Fig. 21). The alarm icon

flashes as a warnin

g

Setting MAX
Set

temperature

t

°C

Controller error

Emergency operation

Fig. 24 Schematic diagram of how the TWW temperature monitoring function

s

T

he monitoring temperature must always be set sufficiently high above the maximum

wor

king

temperature.

f the acoustic alarm is switched on in the SETUP, the TWW alarm is additionally signalled by
an intermittent tone. If the SET key is pressed, the horn can be temporarily switched off until
t

he ne

xt alarm event occurs.

7

A

dvanced function

s

Setting:

g

vertemperature limit:

p to max. 10 °C above nominal temperature

for details of the nominal temperature, see nameplate)

Sett

ing

:

1.

ct the

MAX

icon with the push-turn control.

.

old down the SET key and set the desired temperature limit

with the push-turn control (e.

g

. 38.°C

)

°C

MIN

AUTO

MAX

Undertemperature limit:

A

djustment range: 10 °C below the minimum temperature of the
appliance up to 10 °C above nominal temperature of the appli­ance (for details of the nominal temperature, see nameplate).

The l

ower alarm limit value cannot be set higher than the top

o

ne. If no undertemperature limit is required, set the lowest

t

emperature.

°C

MIN

AUTO

MAX

48

A

dvanced function

s

7.3.3 Automatictemperature monitor (ASF)

A

SF is a monitoring device that automatically follows the set temperature setpoint within an

adjustable tolerance band (

. 25 ).

T

he ASF is activated – if switched on – automatically if the actual temperature value reaches
50 % of the set tolerance band of the setpoint (in the example: 37 °C – 1.5 °C) reached for the
first time (section A). The activation of the ASF is shown by the bri

g

htly lit

AUTO

icon

.

When the temperature moves outside the set tolerance band around the setpoint (in the

e

xample in

. 25: 37 °C ± 3 °C) – e. g. if the door is opened during operation (section B of

illustration) – the alarm is set off. This is shown by the flashin

g

AUTO

and icons.

f the acoustic alarm is switched on in the SETUP, the ASF alarm is additionally signalled by an
intermittent tone. If the SET key is pressed, the horn can be temporarily switched off until the
n

e

xt alarm event occurs

.

T

he ASF alarm goes off automatically as soon as 50 % of the set tolerance band of the set-

point (in the example: 37 °C ± 1.5 °C) are reached a

g

ain (section C).

f the temperature setpoint is altered, the ASF is automatically disabled temporarily (in the

e

xample: The setpoint is changed from 37 °C auf 30 °C, section D), until it has reached the

tolerance ran

g

e of the new temperature setpoint (section E).

40 °C

34 °C

ASF active

40 °C

34 °C

ASF alarm

ASF active ASF active

33 °C

27 °C

°C

37 °C

t

ABCDE

AUTO AUTO AUTO

Fig. 25 Schematic diagram of how the ASF temperature monitoring function

s

Switching on the automatic temperature monitor:

gp

Select the AUTO icon with the push-turn control.

old down the SET key and set to on with the push-turn control.

MIN

MAX

AUTO

°C

Switching off the automatic temperature monitor:

gp

Select the AUTO icon with the push-turn control.

old down the SET key and set to

off

with the push-turn control.

T

he tolerance band for the ASF can be set in the SETUP in the

0.5 ... 5 °C (see page 44 ).

MIN

MAX

AUTO

°C

49

A

dvanced function

s

Sterilisation

ipcar

d

or decontamination, the incubator i

s

e

quipped with a STERICard as standard

. 26). The STERICard starts an

automatic an

d

predetermined sterilisa-

t

i

on sequence.

W

arning

!

The incubator becomes

v

ery hot inside during

the sterilisation

p

roce-

d

ure. Do no open the

doo

r.

C

aution:

Th

e sterilisation programme is not

intended for sterilisin

g

the chamber

load, but only for sterilisin

g

the inte-

ior of the appliance. The incubator

is not a steri

l

iser with respect to the

aw on Medical Devices.

ake sure that the chamber is empty before the sterilisation procedure is started. Sliding

shel

ves and removed water trays may also be sterilised.

To activate,the STERICard, insert it into the chip card reader (see page

10 ) on the operating

panel of the appliance and select the Start icon

with the push-turn control while the SET

ey is held down.

A

s soon as the sterilisation procedure has been enabled, the automatic sterilisation procedure

egins:
amp 1: Heating up to 160 °C
amp 2: Hold time 4 hours (after setpoint temperature has been reached)
amp 3: Cooling down to 70 °C
amp 4 Wait time of 10 minutes

Th

e automatic sterilisation programme cannot be change

d.

A

fter the sterilisation has been completed, STERILISATION OK is shown in the controller display .

or documentation purposes, the sterilisation procedure is logged on the STERICard and can

be read out with a com

p

uter/laptop.

Sterilisation
Process Control
Card

Sterilisation Parameter:
160 °C / 4:00 h

Unit-ID:
________________________

Fig. 26 STERICard

A

dvanced function

s

7.5 User ID card (optionally available as an accessory

)

The de

vice number of the incubator and a

nique user number are stored in

e

ncrypted form on the user ID card (

.

7 ). The user ID card therefore works

onl

y in the appliance with the corre-

s

ponding serial number

.

T

o use it, insert the card into the chip

c

ard reader (see page 10 ) on the appli-

ance operatin

g

panel

.

f the user ID card has been inserted, the

m

e

nu itemID LOCK also appears in the

SETUP menu. If the settin

g

is set to ON

t

h

e appliance is locked against all altera-

tions once t

h

e chip card is removed.

ocking with the user ID card is displayed with the lit up icon on the operating

anel.

very login process with the user ID card is logged in the internal Flash memory of the control-

r.

7.6 Calibrati

on

7.6.1

T

emperature calibratio

n

T

he incubator can be calibrated customer-specifically using three calibration temperatures of

your c

h

oice

:

C

AL.1Temperature calibration at low temperature (to 30°C)

C

AL.2 Temperature calibration at medium temperature (from 30°C to 38°C)

C

AL.3 Temperature calibration at high temperature (over 38°C)

or each selected balance point ( Fig. 28 ) a positive or negative compensation correction value

c

an be set between -2.9 °C and +2. 9 °C

There must be a difference of at least 10 ºC between

e

ach of the individual balance points.

CAL. 1

+0,2 °C

CAL. 3

+0,3 °C

CAL. 2

-0,4 °C

Default

calibration

30°C

20°C

0°

10°C

40°C

Fig. 28

emperature calibration (example)

access
authority
card

Name:

ID:

_______________ ______
_______________ ______

Fig. 27 User ID Card

1

A

dvanced function

s

Setting:

g

et compensation temperature in the SETUP (see page

) and set accompanying com

-

ensation correction value to

°C.

With a reference instrument, measure the deviation in the stationary state in the selected

c

ompensation temperature.

3

.

et the compensation correction value in the SETUP. If the measured reference tempera-

t

ure is too low, the compensation correction value must be set with a negative sign.

4

.

rform a control measurement with the reference instrument

.

5.

epeat the procedure for the other two balance points if necessary .

xample: The temperature deviation in the chamber load at 35 °C should be corrected.

1.

et the compensation temperature CAL.2 in the SETUP to 35.

°C and the accompanyin

g

c

ompensation correction value to. °C:

h

°C

°C

. With a calibrated reference instrument and at a set setpoint temperature of 35 °C in nor

-

al operation, an actual temperature of 34.6 °C is measured.

3

.

et compensation correction value for CAL.2 in the SETUP to - 0.4 °C:

h

°C

°C

4

. The reference instrument after the calibration procedure should now display 35 °C

5. With CAL.1 another compensation temperature below CAL.2 can be programmed in the

s

ame way , and with CAL.3

one lying above this.

f all compensation correction values are set to

°C, the factory calibration settings are

r

estored

.

A

dvanced function

s

7.6.2

umidity calibration

T

he incubator can be calibrated for the individual customer by means of two balance points

at 20 an

d

at 90 % relative humidity . F or each selected balance point a positive or negative

c

ompensation correction value can be set between –5 % and +5 %.

rh 20

+3,0 %

rh 90

-2,0 %

60%

40%

0%

20%

80% 100%

Default calibration

Fig. 29 Humidity calibration (example)

Setting:

g

1.

et the desired humidity balance point in the SETUP (see page 44 ) and set accompanyin

g

c

ompensation correction value to0.0 °C.

. With a reference instrument, measure the deviation in the stationary state in the selected

umidity balance point.

3

.

etting the compensation correction value in the SETUP. If the measured reference humid-

ity is too

l

ow, the compensation correction value must be set with a negative sign.

4

.

rform a control measurement with the reference instrument

.

T

he procedure can be performed with humidity balance points of 20% rh and 90% rh.
xample: Humidity deviation at 90 % should be corrected.

1.

et humidity balance point in the SETUP to RH 90 and set the accompanying compensation

corre

ction value t

o

0.0

%rh

:

PRINT

SETUP

loop

t3

t4

t2

t1

on
off

Mo

Tu

We

Th

Fr

Sa Su

3

4

2

1

STERI DEFRO

°C

°C

rh

mb

%

CO
mb

2

IN 1

IN 2

OUT

MIN

AUTO

MAX

. With a calibrated reference instrument, an actual humidity of 88% rh is measured at nor-

al operation, with a set setpoint humidity of 90 %rh

.

3

.

et the compensation correction value in the SETUP for RH 90 to –2.0 %rh

:

PRINT

SETUP

loop

t3

t4

t2

t1

on
off

Mo

Tu

We

Th

Fr

Sa Su

3

4

2

1

STERI DEFRO

°C

°C

rh

mb

%

CO
mb

2

IN 2

OUT

IN 1

IN 2

OUT

MIN

AUTO

MAX

A

dvanced function

s

4

. The reference instrument should display 90.0% rh after the calibration procedure.

With

R

H 20 a further comparison can be programmed at 20 % relative humidity .

f all compensation correction values are set to

% rh, the factory calibration settings

ar

e restored

.

7.6.3

calibrati

on

ustomer-side calibration of appliance on controller by means of three C

O

2

points:

C

O

calibration at 5 % CO conten

t

C

O2 10 C

O

2

calibration at 10 % C

O

2

conten

t

C

O2 15 CO calibration at 15 % C

O

c

ontent (only for models with

C

O

or premium modules

)

or each selected balance point a positive or negative compensation correction value can be

set

.

co2 5

-0,4 %

20%

0%

10%

co2 10
+1,5 %

co2 15

-0,7 %

Default calibration

Fig. 30

CO

2

calibration (example)

Setting

g

5.

et the desired C

O

2

balance point in the SETUP (see page 44 ) and set accompanyin

g

c

ompensation correction value to 0.0 % rh.

. With a reference instrument, measure the deviation in the stationary state in the selected

2

balance point.

et the compensation correction value in the SETUP. If the measured reference CO

content

is too

l

ow, the compensation correction value must be set with a negative sign.

.

rform a control measurement with the reference instrument

.

. The procedure can be performed for the CO2 balance points 5 %, 10 % and 15 %

15 % only for models with CO2or premium modules).

4

A

dvanced function

s

xample: C

O

d

eviation in chamber load at 10 % should be corrected:

1.

et the C

O

bal

ance point in the SETUP to CO2 10 and set the accompanying compensation

corre

ction value to.%:

PRINT

SETUP

loop

t3

t4

t2

t1

on

off

Mo

Tu

We

Th

Fr

Sa Su

3

4

2

1

STERI DEFRO

°C

°C

rh

mb

%

CO

mb

2

IN 1

IN 2

OUT

IN 1

IN 2

OUT

MIN

AUTO

MAX

. With a calibrated reference instrument and at a set CO content of 10.0 %, an actual C

O

c

ontent of 11.5 % is measured.

3

.

et the compensation correction value in the SETUP for CO2 1

0

to

1.5 %:

PRINT

SETUP

loop

t3

t4

t2

t1

on

off

Mo

Tu

We

Th

Fr

Sa Su

3

4

2

1

STERI DEFRO

°C

°C

rh

mb

%

CO

mb

2

IN 1

IN 2

OUT

IN 1

IN 2

OUT

MIN

AUTO

MAX

. The reference instrument should display 10 % after the calibration procedure.

Wit

h

CO2 5

and CO2 15 further calibrations can be programmed at 5 % and 15 % (15 % only

f

or models with C

O

2

or premium modules).

f all compensation correction values are set to0.0%, the factory CO2 calibration settings

ar

e restore

d.

7.6.4 Oxygen calibration

only for models with O2 module

)

n the

O

2

calibration, the oxygen proportion in the incubator at normal atmosphere is deter-

min

ed

.

1.

witch off the

O

2

module, if active. To do this, turn the
ush-turn control to the left until the O2 display flashes.
old down the set-key and turn the push-turn control
ntil

O

2

off

is displayed. Release the set-key .

.

et CO setpoint value to 0% (see page 31 ).

3

.

pen the oven door and inner glass door , in case of

f

our-part door, remove glass panes. Keep the doors

o

pen for at least one minute so that a normal atmos

-

here can form in the chamber . Close the doors again

.

4

.

n the setup, select the item O2 calibration with the

ush-turn control

(

c

al 02 of

f

). Hold down the set key

and set to cal 02 on with the push-turn control. Release

th

e set key and leave the setup via EXIT.

ow, the oxygen content in the incubator is measured

f

or about one minute. In the display , cal o2 acti

ve

can be

s

een. Do not switch off the oven while this is taking

ace.

5. When the calibration is finished, the oxy

g

en content

d

etermined is shown in the display:

on
off

Mo

Tu

We

Th

Fr

Sa Su

o2 off

off

CAL o2 on

CAL o2 active

o2=20.7%

A

dvanced function

s

Communicationinterfaces

only for models with communication or premium modules

)

f the incubator is equipped with the communication or premium module, its log data can be

rea

d

out with a computer/laptop. For this purpose, the incubator has corresponding commu

-

nication interfaces on the rear of the appliance (see page 13 ).

T

o be able to use the RS 232, RS 485 And USB interfaces, the incubator must be assigned a

nique device address in the SETUP, menu item ADDRESS (see page

4), via which the computer

c

an communicate with the oven. The default setting is ADDRess 0 Using this address, the ap-

propriate incubator can be selected from the computer .

7.7.1 Communication Interface

S-232 C in accordance with DIN 12900-1

T

he computer can be connected with a shielded interface

c

able to the 9-pin interface on the rear of the appliance (see

. 31

and page 13). The shielding must be connected to the plug casing.

f the serial port is not used, put on the cover included.
f several ovens are to be connected to a computer via RS-232-C

interfaces, an appropriate interface on the computer and a separate

c

able are required for each oven (

. 32 ). The maximum cable

ength is 15 m.

Pin allocation:

1

3

4

not oc

-

c

upie

d

x

D

TxD

not oc

-

c

upie

d

GN

D

not oc

-

c

upie

d

not oc

-

c

upie

d

oc-

c

upie

d

oc-

c

upie

d

RS 232

9-pin serial port

12345

6987

Fig. 31 RS-232-C

interface

A

dvanced function

s

Fig. 32 Connecting incubators via the RS 232-C communication interface

7.7.2

us interface

S 485

f requested on the corresponding order , the oven can be fitted in the factory with a RS-485

interface instead of a RS-232-C interface. This enables the networkin

g

of several ovens (up to

16) with one computer via a shared two-wire cable (

. 34 ). The chamber must be given a

nique device address in the SETUP submenu, menu item

ADdRESS

(see page 44 ), via which the

c

omputer communicates with the oven. A maximum of 16 devices can be addressed on the

S-485 bus.

T

e default setting is ADDRess 0 Using this, the appropriate incubator

c

an be selected and programmed from the PC.

T

he computer needs to be equipped with either an RS-485 interface

o

r with an RS 232/RS 485 converter (

. 34). Connecting the wires

is done individually with a shielded cable, dependin

g

on the installa-

tion site. The maximum total cable len

g

th is 150 m.

220 ohm terminating resistor must be connected to the last device.

Pin allocation:

1

3

4

not oc

-

c

upie

d

not oc

-

c

upie

d

not oc

-

c

upie

d

not oc

-

c

upie

d

not oc

-

c

upie

d

not oc

-

c

upie

d

oc-

c

upie

d

RS 485

9-pin serial port

12345

6987

Fig. 33 RS-48

5

interface

g

7

Advanced function

s

RS232

RS485

Interface converter

Fig. 34 Connecting incubators via the RS485 bus interface

7.7.3

SB interfac

e

f several chambers are to be connected to a computer via USB interface, an appropriate

interface on the com

p

uter and a separate cable are required for each chamber . The maximum

cable length

is 5 m.

7.7.4

thernet interfac

e

LAN 1: 192.168.1.233

192.168.1.216

LAN 2: 192.168.1.215

LAN 3: 192.168.1.241

Fig. 35

Connecting one or more incubators to a network using an Ethernet interface

(schematic diagram)

g

g

g

A

dvanced function

s

or identification purposes, each appliance connected must have its own unique IP address.

ach chamber is delivered by default with the IP address 192.168.100.100. The program

XTADMIN”, which can be found on the CD-ROM provided, can be used to change the IP ad-

dre

ss.

Settin

g

the IP address is described in a separate manual.

7.8 Logmemor

y

only for models with communication or premium modules

)

Th

e controller continually logs all relevant measured values, settings and error messages at

1-minut

e

intervals.

Th

e internal log memory is listed as a ring memory , i. e. the oldest log data are always over-

written automatica

ll

y with new data.

T

he logging function cannot be switched off, but is always active. The measured data are

s

tored in the controller , safe from manipulation. Each dataset is stored with a unique times-

t

amp.

T

he internal log memory has a size of 1024 kB. This corresponds to a storage capacity for

a

b

out three months in permanent operation.

f the power supply is interrupted, the time of the power cut and the return of voltage are

stored

in the contr

olle

r.

Reading in the log memory to the PC via USB interface

ggy

or documentation purposes, log data can be read out via an interface.

T

he log memory of the controller is not modified or deleted by the reading out.

Printing out log memory

gg y

see also Chapter “

“ on page

43

)

or a printout, the GLP header is also printed automatically , and contains the following details:

ate of printout

eriod of lo

g

onsecutive page numbers

erial numbers and appliance nam

e

f the printer is not ready (e. g. ink cartridge or paper tray empty), no log data will be

lost. Multiple printouts can also be made, since the lo

g

memory is not deleted after print

-

n

g.

aintenance and Servicing

. Maintenance andServicin

g

.1

eanin

g

W

arning

!

anger of injury. Before any cleaning work, pull out the mains plug.

egular cleaning of the easy-to-clean chamber interior prevents
build up of material remains, which over time could impair
the appearance and functionality of the stainless steel

chambe

r.

T

o clean the interior , the fan cover can be removed by pulling

it sli

g

htly forwards, after the two fixing screws have been

s

crewed out (

. 36 ). The protective grid of the O

sensor c

an

if the model is equipped with a O

module) be taken off after

the fixin

g

screws have been removed (

. 37).

The me

tal surfaces of the chamber can be cleaned with nor-

mal stainless steel cleanin

g ag

ents. Make sure that no rusty

ob

jects come into contact with the working chamber or with

the stainless steel housin

g

. Rust deposits lead to an infection

o

f the stainless steel.

f rust spots should appear on the surface of the working

chamb

er , due to impurities, immediately clean and polish

the

affected area.

o not clean the operating panel, the plastic input module

and other

p

lastic parts of the chamber with caustic or solvent-

ased cleaning agents

.

.2

egular maintenanc

e

Monthly

y

or models with O module: Perform oxygen calibration (see page

4 ).

Every three months

y

heck that door seals fit tightly , adjust door if necessary (see page

).

n permanent mode: Grease the moving parts of the doors (hinges and lock) with thin

sil

icone grease and check that the hinge screws are not loose.

Annually:

y

Grease the moving parts of the doors (hinges and lock) with thin silicone grease and check

th

at the hinge screws are not loose.

heck that door seals fit tightly , adjust door if necessary (see page

).

Fig. 36 To take off th

e

fan cover, remove two

g

scre

ws

Fig. 37

O

2

sensor

aintenance and Servicing

.3 Adjusting doo

r

A

well-closing door is indispensable for incubators.

n Memmert appliances, the tight closing of

t

he d

oor is optimally guaranteed by a chamber

s

eal and a door seal (see also page 10 ). In

permanent operation, it is possi

bl

e that the

flexible seal material will be

g

in to sag. To ensure

t

h

at the door closes exactly despite this, an

adjustment may be necessary (

. 38 ).

T

he top section (1) of the door hinge can be
moved sli

g

htly in the direction of the arrow after

the two screws (2) at the to

p

and bottom of the

d

oor have been undone. Afterwards, tighten the

s

crews down again

.

Ad

justing the door:

1. Undo the ma

gg

ot screw (5) (with a jolting

otion, as it is fixed with locking paint).

. Adjust the door by turning the eccentric (3)

wit

h

a screwdriver.

3

. Apply locking paint to the maggot screw

an

d

re-tighten it.

T

he locking plate (

. 39 ) can also be adjusted

in the direction of the arrows after undoin

g

the

s

crew (6).

1. Undo screw (6).

.

ove locking plate (7) in direction of arrow.

3

. Tighten the screws again

.

.4 Repairs andServic

e

W

arning

!

A

fter removing covers, live
arts may be exposed. You

ay receive an electric shock
f you touch these parts. Disconnect the mains plug before remov­ng any covers. Any work inside the unit may only be performed by

q

ualified electricians.

epairs and service work are described in a separate service manual for the INCO

incu

bato

r.

2

1

3
4

5

Fig. 38

Adjusting door

jg

Mounting bolts

pp

3

Eccentric tappet

Eccentric socket

pp

p

5

Headless screw

7

6

Adjusting locking plat

e

6

Screw

7 Locking plat

e

1

torage and Disposal

. Storage and Disposa

l

.1 Stora

ge

T

he incubator may only be used under the following conditions.

ry and in an enclosed, dust-free room

rost-fr

ee

isconnected from the power supply and gas supply

Undo

g

as bottle connections and close valves of gas bottles.

Gas bottles may be stored in closed rooms if these are sufficiently well ventilated.

For appliances with basic fittings:

pp g

emove, empty and clean the water tray(s).

For appliances with the humidity module:

pp y

Undo hose connection of water supply tank; empty water tank.

.2

isposa

l

T

his product is subject to the Directive 2002/96/EC on Waste Electrical
lectronic Equipment (WEEE) of the European Parliament and of the EU

ouncil of Ministers. This appliance has been brought to the market

after 13th Au

g

ust 2005 in countries which have already integreated
this directive into their national laws. It may not be disposed of in
norma

l h

ousehold waste. To dispose, please contact your dealer or

manufacturer . Any appliances that are infected, infectious or contami

-

nated with materials that are a hazard to health are excluded from

eing taken back. Please observe the other regulations in this context.

efore disposing of the appliance, please render the door locking

mechanism unusable, for example, to prevent playin

g

children from

eing locked inside the appliance.

y

T

he appliance may not be left at public or communal recycling or collection points.

nd

ex

Index

Accessorie

s 1

8

A

ccidents 9

A

coustic signal 4

4

Add

itional modules 12

Ad

justing door 60

A

dvanced functions 43

Ambie

nt conditions 1

8

Appl

iance error 39

A

SF 4

8

A

utomatic temperature

nitor 4

8

ase 21
asic device settings 43
asic equipment 11

C

alibration 44,

amber load 25
anges

8

cks 2

2

ipcard 49

eaning 5

9

ck time 44
2 calibration 5

3

O2 setpoint 31

mmunication address 4

4

mmunication interfaces

5

ompensation temperature

1
onformity 15
onnecting 2

2

onnection of external appli-

anc

e

s 14

nnections 1

3

nversions

8

rrective values 44,

1,

ustomer service

2

ate 44
eclaration of conformity 1

5

ecommissioning 6

1

elivery 19,
esign 10

mensions 1

7

isposal 61

r 25
r seals 59

C Declaration of Conform

-

ty 15

ctrical connection 14
ectrical equipment 13
ectronic temperature moni-

toring

46

mergency

9

nding operation 37
NDSOUND 44
rrors 3

9

thernet interface 57
xplosion protection 14
xternal devices 22
xtras 11

unction 11

Gas connection 14
Gas supp

l

y 26

azards 7

w to set the IP address 5

8

umidity calibration 52,

cons used

6

nten

ded

use 14
nterface cable 43
nterface mode 2

8

nterfaces 55
rregularities

8

anguage 4

4

aptop 34
ength of lead 57
oading 25
og memory 4

2

,

43

ain switch 2

6

aintenance 59
alfunctions

8

,

38

anufacturer

2

aterial 13
emory 5

8

essages 3

8

inimum clearances 2

0

onitoring temperature 45

ameplate 15

rmal m

ode

2

8

,

2

9

O

2 17
2 module 12
2 setpoint 31
perating modes 28
perating mode - settings

2

8

perating panel 27
perating personnel 8,

5
peration 25
peration with PC/laptop 34
perator 25
xygen calibration 45,

4

ackaging material 1

9

arameters 27
C 34

rformance 1

6

wer failure 42
ower supply 22
rinter 4

3

rinting out log memory 5

8

roduct safety 7
t100 temperature sensor

4

5

ush/turn control 2

6

utting into Operation 22

altime clock 45

egular maintenance 5

9

ng memory 5

8

S-232 55
S 485 5

6

nd

ex

Safety regulations 6
Serv

i

ce 6

0

Servicing 5

9

,

Setting parameters 27
Settin

g

s example norma

l

ode 3

0

Settings example week time

s

witch 33

Setu

p

19, 2

0

,

43

Stacking frame 2

1

Standard accessories 1

8

Storage after delivery 20
Stora

g

e capacity 58

Switchin

g

off 26

Switchin

g

on 26

System impedance 22

T

Tech

nical data 1

6

T

emperature calibration 50,

1

T

emperature comparison 5

0

T

emperature deviation 51

Temp

erature limiter (TB) 45

Temp

erature monitor 48

T

emperature monitoring 45

T

emperature monitorin

g

TWW) 46

Temp

erature sensor 45

Te

rms used

6

Tole

rance band ASF 44

T

ransport 1

9

T

ransport damage 1

9

T

WW 4

6

Unpacking 1

9

SB interface 57
ser ID card 5

0

W

Warning messages 4

9

Water connection 22
Water tray(s) 26
Wee

kd

ay 44

W

eek

time switch 2

8

,

31

Wei

gh

t 16

What t

o do

in case of ac-

cide

nts 9

Ye

ar 44

Memmert GmbH + Co KG | Postfach 1720 | D-91107 Schwabach | Tel. [phone] | Fax [phone] | E-Mail: [e-mail] | www.memmert.com

05.11.2014

INCO/INCO med englisch

D23532