Lonza Lucetta 2 User Manual

Bioscience Solutions

Lucetta™ 2 Luminometer Manual

Contents

1.0 Introduction 5

1.1 Lucetta™ 2 Luminometer Overview 5

1.2 Light Detection 5

2.0 General Use Instructions 5

2.1 Use Restrictions 5

2.2 Maintenance 5

2.3 Safety Instructions and Use Precautions 6

2.4 Storage Conditions 6

2.5 Batteries 6

2.6 Return Shipment 7

2.7 Instrument Disposal 7

3.0 Setup Instructions 7

3.1 Lucetta™ 2 Luminometer Components 7

3.2 Setup Instructions 8

3.3 Optimal Operation Temperature 8

3.4 Battery Operation 8

3.5 Electrical Outlet Operation 8

3.6 Consumables 8

3.7 Quality Control 8

Lucetta™ 2 Luminometer Manual – Bioscience Solutions 3

4.0 Lucetta™ 2 Luminometer Operation 9

4.1 Menu 9

4.2 MycoAlert™ PLUS/ MycoAlert™ Run 9

4.3 Single Read Run 10

5.0 Data Storage and PC Upload 11

5.1 Maximum Storage Capacity 11

5.2 Viewing Logged Measurements 11

5.3 Upload to a PC 11

6.0 Setting Instrument Parameters 12

6.1 Password 12

6.2 Customer Settings 12

6.3 Date & Time 13

6.4 System 13

6.5 Information 13

7.0 Troubleshooting 14

8.0 Error Codes 14

9.0 Appendix 14

9.1 Factory Settings for Single Read 14

9.2 Technical Data 15

4 Bioscience Solutions – Lucetta™ 2 Luminometer Manual

1.0 Introduction

1.2 Light Detection

1.1 Lucetta™ 2 Luminometer Overview

The Lucetta™ 2 Luminometer is a single tube luminometer developed
for the detection of bioluminescence and chemiluminescence. It can
be used as a portable battery-operated instrument as well as with an
external power supply and is suitable for detecting all luminescence.

Highly sensitive luminescence detection

The Lucetta™ 2 Luminometer is able to detect less than 3 fmols
ATP. The sample container is positioned beside the photon counting
photomultiplier and the read chamber is sealed to avoid light leakage.
Close proximity of the sample container to the highly sensitive photon­counting photomultiplier maximizes light collection efficiency and
allows the detection of minimal light intensities.

Robust and reliable construction

The Lucetta™ 2 Luminometer is a lightweight and compact instrument
with a measuring chamber for sample intake. After loading a sample, the
measuring chamber lid is closed and the measurement automatically
begins.

Stand-alone instrumentation

The Lucetta™ 2 Luminometer can be utilized as a stand-alone
instrument without a PC. It features on-board software with menu
options to optimize sample measurements (MycoAlert™ PLUS and
MycoAlert™ Mode or Single Read mode), upload data to a PC and
configure system parameters.

Two basic measurement modes

The easy to operate instrument-embedded software is tailored around
two basic measurement modes:

– MycoAlert™ PLUS and MycoAlert™ Mode: straight-forward

implementation of the procedure for running Lonza’s MycoAlert™
PLUS and MycoAlert™ Mycoplasma Detection Assays.

– Single Read mode: allows the user to obtain unprocessed

luminescence readings. This mode can be used for Lonza’s
ViaLight™ or ToxiLight™ Assay or other luminescent assays.

Data storage capacity and transfer

– Each completed measurement is stored in the instrument’s

internal circular memory with a storage capacity of up to 2,000
single sample readings.

– Furthermore, sample results may be uploaded to a PC to view and

analyze results with Microsoft® Excel®. The data transfer to PC is
enabled by the Lucetta™ Connect software.

Broad range of applications

Lucetta™ 2 Luminometer applications include:

– MycoAlert™ PLUS and MycoAlert™ Mycoplasma Detection Assays
– ATP assays, e.g. ViaLight™ Cell Proliferation and Cytotoxicity Assay
– Adenylate Kinase assays, e.g. ToxiLight™ Non-destructive

Cytotoxicity Assay

– Luciferase reporter gene analysis

The photomultiplier (detector) is a single photon counter, which
measures light ranging from 380 to 630 nm. The photons emitted
by the sample are converted into electrons and amplified by the
photomultiplier. The individual impulses are counted digitally and are
directly proportional in their linear range to the emitted amount of
light.

Luminescence is noted in Relative Light Units (RLU). For MycoAlert™
and MycoAlert™ PLUS measurements, the Lucetta™ 2 Luminometer
reports RLU/s values. For Single Read measurements, the Lucetta™ 2
Luminometer reports in RLU values. The readout is integrated over the
measurement period where RLU values in time intervals of 5 ms are
gathered.

2.0 General Use Instructions

2.1 Use Restrictions

The Lucetta™ 2 Luminometer is intended for research and
investigational use by professionals only. Please note that it is not
intended to be used for diagnostic purposes or for testing or treatment
of humans.

The Lucetta™ 2 Luminometer may only be used for measurement of
glow type bio- and chemiluminescence of samples in tubes.
The instrument may not be used for purposes other than those
described above.

2.2 Maintenance

The Lucetta™ 2 Luminometer is low maintenance. It should be protected
from dust and debris.

The surface of the instrument is protected by a robust and washable
finish. Should it become dirty or dusty, we recommend wiping it with a
damp cloth or optical grade tissue. If necessary, a mild, non-abrasive
detergent can be used. The instrument can be cleaned by wiping
with 70% (v/v) ethanol if required. Acetone damages the instrument
coating and should NOT be used for cleaning!

The sample holder must be kept clean and wiped with a damp cloth if
dirty.

No free fluid should ever enter the instrument! If this occurrs,
disconnect the instrument immediately, remove the test tube and
turn the instrument upside down to remove the fluid before it enters
the measuring chamber. Contact the Lonza Scientific Support Team
immediately!

Lucetta™ 2 Luminometer Manual – Bioscience Solutions 5