Lonza Amaxa Nucleofector II User Manual

Amaxa™ Nucleofector™ II Manual

For more information and future program updates,
check www.lonza.com/nucleofection-program-update

Nucleofector™ II Chip Card

For future protocol updates, also check www.lonza.com/cell-database

Nucleofector™ II CD-ROM

Contents

1.0 The Amaxa™ Nucleofector™ Technology 3

2.0 Operating Instructions 5

1

2.1 Restrictions 5

2.2 Maintenance 5

2.3 Safety Instructions–Please Read Carefully 6

2.4 Waste Disposal 7

2.5 Nucleofector™ II Device Components 7

2.6 Set-up Instructions 7

2.7 Instructions for Using the Nucleofector™ II Device 8

2 .7. 1 Main menu and program execution 8

2.7.2 Selection of Nucleofector™ Programs by program code–“Free Program Choice” 8

2.7.3 Selection of Nucleofector™ Programs by name of cell type–“Cell Type List” 8
2 .7. 4 Selection and storage of Nucleofector™ Custom Programs 9

2.7.5 Program execution 9

2.8 Additional Nucleofector™ II Settings and Options 10

2.8.1 Change of standard settings 10

2.8.2 Motor 10

2.8.3 Display contrast 10

2.8.4 Beep on/o 10

2.8.5 Change of start mode 10

2.8.6 Language 11

2.8.7 Software version 11

2.8.8 Upgrading program software 11

2.8.9 Serial number 11

2.8.10 96-well mode 11

Contents

2

3.0 Troubleshooting 12

4.0 Nucleofector™ II Device Error Codes 14

5.0 Appendix 16

1.0 The Amaxa™

Nucleofector™
Technology

3

Since its introduction in 2001, a constantly growing interest in
the scientic community and industrial markets established
the Amaxa™ Nucleofector™ Technology as a leading transfection
Technology all around the world. A continuously increasing number
of publications from many dierent research areas reect how the
Nucleofector™ Technology drives research in numerous dierent
cell types and applications.

The Nucleofector™ Technology oers you more opportunities than
any other transfection method to approach your research topics
from many dierent angles.

Transfection of virtually any cell

With over 100 protocols for cell lines optimized by Lonza’s R&D
team, more than 500 entries in the online cell data base and
almost 50 Nucleofector™ Kits for dierent primary cells, the
Nucleofector™ Technology is clearly the transfection method-of­choice for virtually any dicult-to-transfect cell type.

Transfection of any substrate

The Nucleofector™ Technology oers high exibility with respect
to applications, since the same transfection parameters apply
for any nucleic acid substrate used. DNA vectors, e.g., expression

plasmids or shRNA vectors, and RNA, e.g., siRNA duplexes, can
be transfected using our transfection protocols. It is the ideal
tool to help you answer the scientic questions in your day-to­day applications such as over-expression studies, gene silencing
approaches, protein expression, generation of stable clones and
many more.

Constant Innovation

At Lonza, we believe that listening to our customers needs is the
best way to develop innovative products that exactly meet your
demands. In line with this dedication, the Nucleofector™ II Device
was developed which oers a larger graphic display, a motor­driven carousel, the possibility to store individual programs for
optimized cell lines and to perform bacterial transformations.
In addition, the Nucleofector™ II Device serves as the program
delivery unit for Lonza’s Nucleofector™ 96-well Shuttle™ System,
a 96-well transfection unit that allows e.g., high throughput
transfection of siRNA and cDNA libraries in virtually any cell,
including primary cells.

What has been our mission in the past will continue to be so in
the future–providing you with innovative transfection solutions to
help you thrive and excel in your research.

The Amaxa™ Nucleofector™ Technology 1.0

4

Components of the Nucleofector™ Technology

The Nucleofector™ Technology is based on two unique components,
the Nucleofector™ Device that delivers the specically developed
electrical parameters and Nucleofector™ Kits that contain cell-
specic and optimized Nucleofector™ Solutions.

The Nucleofector™ II Device with Kit Components

As part of the Nucleofector™ Technology, Lonza provides cell-type
specic Amaxa™ Optimized Protocols for many dierent cell lines
and primary cells. An up-to-date database of the continuously
growing range of Amaxa™ Optimized Protocols can be found on our
homepage at www.lonza.com/cell-database.
If no Amaxa™ Optimized Protocol for your cell line of interest is
available, we oer a Cell Line Optimization Nucleofector™ Kit
enabling you to easily establish the Nucleofector™ Technology
for your specic cell line. For ne-tuning after the optimization
process, please contact our Scientic Support Teams.

Phone Europe/World: [phone]
USA: 800-521-0390

E-mail Europe/World: scienti[e-mail]
USA: scienti[e-mail]