LaMotte TRACE ELEMENT PLANT TISSUE KIT SAP SPOT TEST User Manual

Mott

TOTAL HARDNESS TEST KIT

DIRECT READING TITRATOR METHOD

MODEL PHT-DR-LT • CODE 4482-DR-LT

QUANTITY CONTENTS CODE

15 mL *Hardness Reagent #5 *4483-E 100 Hardness Reagent #6 Tablets 4484-J 60 mL Hardness Reagent #7 4487DR-H 1 Test Tube, 5-10-12.9-15-20-25 mL,

1 Direct Reading Titrator, 0-200 Range 0382 1 Pipet, 0.5 mL, plastic 0353

*WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by email, phone or fax.

To order individual reagents or test kit components, use the specified code number.

NOTE: Read the Direct Reading Titrator Instruction Manual before proceeding. The Titrator is calibrated in terms of total hardness expressed as parts per million (ppm) calcium carbonate (CaCO division on the Titrator scale equals 4 ppm CaCO

glass, w/cap

). Each minor

0608

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

PROCEDURE

1. Fill the test tube (0608) to 12.9 mL line with sample water.

2. Add five drops of *Hardness Reagent #5 (4483). Mix.

3. Add one Hardness Reagent #6 Tablet (4484). Cap and swirl until

tablet disintegrates. Solution will turn red.

4. Fill the Direct Reading Titrator (0382) with Hardness Reagent #7 (4487DR). Insert Titrator in the center hole of the test tube cap.

5. While gently swirling the titration tube, slowly press the plunger to add Hardness Reagent #7 (4487DR) until the red color changes to blue. Read the test result directly from the scale where the large ring on the Titrator meets the Titrator barrel. Record as ppm Total Hardness as CaCO

EXAMPLE: Plunger tip is 3 minor divisions below line 80. Test result is 80 plus (3 divisions x 4) equals 92 ppm.

6. If the plunger tip reaches the bottom line on the Titrator scale (200 ppm) before the color change occurs, refill the Titrator and continue the titration. When recording the test result, be sure to include the value of the original amount of reagent dispensed (200 ppm).

7. To convert ppm Hardness to grains per gallon (gpg), multiply by

0.058. Record as gpg Hardness as CaCO

gpg CaCO

= ppm CaCO3x 0.058

ANALYSIS OF HARDNESS IN SALT WATER

When sea and estuarine waters containing very high levels of mineral salts aretested,thesamplemustbediluted before titration. This test kit contains a calibrated pipet for performing the dilution described below.

1. Use the 0.5 mL pipet (0353) to transfer 0.5 mL of the salt water sample to the test tube (0608).

2. Dilute to the 12.9 mL line with distilled water (a 1 to 25.8 dilution).

3. Follow Steps 2 through 5 above. Multiply the Titrator reading by

25.8. Record as ppm Total Hardness as CaCO

LaMOTTE COMPANY

Helping People Solve Analytical Challenges

PO Box 329 • Chestertown • Maryland • 21620 • USA

800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax: 410-778-6394

Visit us on the web at www.lamotte.com

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