LaMotte ST-P User Manual

PHOS PHO RUS IN SOIL

MODEL SL-P · CODE 3340

QUANTITY CONTENTS CODE

60 mL *Acid Extracting Solution *6361-H
60 mL *VM Phosphate Reagent *4410-H
30 mL Charcoal Suspension 5638-G
5 mL *Reducing Reagent 6405-C
1 Pipet, 0.5 mL, plastic w/cap 0369
2 Pipet, 1.0 mL, plastic w/cap 0354
1 Pipet, 0.5 mL, plastic w/cap 0353
1 Pipet, plain, plastic 0352
1 Spoon, 1.0 g, plastic 0697
2 Test Tubes, 5-10-15 mL, plastic, w/caps 0701
1 Demineralizer Bottle 1151
1 Funnel, plastic 0459
1 Filter Paper 0465-H
2 Test Tubes, 2.5, 5 & 10 mL, plastic w/cap 0106
1 Octa-Slide Viewer 1100
1 Octa-Slide Phosphorus 3423
1 LaMotte Soil Handbook 1504

WARNING: Reagents marked with a * are considered hazardous substances.
Material Safety Data Sheets (MSDS) are supplied for these reagents. For your
safety read label and accompanying MSDS before using.

To order a complete set of refill reagents, order as R-3340. To order individual
reagents or test kit components, use the specified code number.

The Demineralizer Bottle provides all of the deionized water used in the
test. Read the Demineralizer Bottle instructions before proceeding.

USE OF THE OCTA-SLIDE VIEWER

The Octa-Slide Viewer should be held so
non-direct light enters through the back of the
comparator. With sample tube inserted at top,
slide the color bar through the viewer and match
with color standards.

PRO CE DURE

NOTE: It is suggested for best possible results to carry a reagent blank
through the procedure to compensate for any color which may develop
within the reagents. The reading from the reagent blank is subtracted
from the reading of the test sample.

1. Use the 1 mL pipet (0354) to add 1 mL of *Acid Extracting
Solution (6361) to a test tube (0701). Add deionized water to the
15 mL line.

2. Use the 1.0 g spoon (0697) to add 3 measures of soil.

3. Use the 0.5 mL pipet (0353) to add 0.5 mL of Charcoal

Suspension (5638). Cap vial and shake for 5 minutes.

4. Use the funnel (0459) and filter paper (0465) to filter solution.
Collect the soil filtrate from both tubes in one vial (0989).

5. Use the 1.0 mL pipet (0354) to add 1.0 mL of the soil filtrate to a
test tube (0106).

6. Dilute the filtrate to the 5 mL line with deionized water.

7. Use the 0.5 mL pipet (0353) to add 0.5 mL of *VM Phosphate

Reagent (4410). Cap tube and mix by inverting the tube several
times.

8. Wait for 5 minutes.

9. Use the plain pipet (0352) to add 2 drops of *Reducing Reagent

(6405). Cap and mix. Solution should turn blue in 10 seconds.

10. Insert test tube into the top of the Octa-Slide Viewer (1100). Slide
the Phosphorus Octa-Slide (3423) into the Viewer. Match sample
color to a color standard. Record as lb/acre Phosphorus.

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Challenges

PO Box 329 • Chestertown • Mary land • 21620 • USA

800-344-3100 • 410-778-3100 (Out side USA) • Fax [phone]

Visit us on the web at www.lamotte.com

SM

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