LaMotte SOIL POTASSIUM DEMONSTRA TION User Manual

SOIL PHOS PHO RUS

L

M

o

tt

DEM ON STRA TION TEST KIT

STU DENT IN STRUC TIONS

CODE 5522/5655

CON TENTS CODE

*Phos pho rus Ex tracting So lu tion *5704
*Phos pho rus In di ca tor Re agent *5705
*Phos pho rus Test Tab lets *5706
High Fer til ity Soil 5724
Color Charts, Phos pho rus 1372
Test Tube, 1-8 mL, plas tic, w/cap 0755
Pipet, trans fer, plas tic 0364
Spoon, 0.5g, plas tic 0698
Soil Sam ple Bags, w/In struc tions 0615
A Study of Soil Sci ence 1530
Soil Anal y sis Re port Form 1582

*WARNING: Reagents marked with an * are considered hazardous substances.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see
MSDS CD or our web site. To obtain a printed copy, contact us by e-mail,
phone, or fax.

To order individual reagents or test kit components, use the specified code
number.

(over)

IN TRO DUC TION

The importance of phosphorus to plant life has been outlined in the
accompanying handbook, A Study of Soil Science. In this exercise the
student will:

A. Study the color reaction of High Fertility Soil (5724) which contains

a high phosphorus content.

B. Test a soil sample of unknown value to determine if phosphorus is

present and if so, determine the relative level of phosphorus that is
present in the soil sample. The soil samples used may be furnished by
the instructor or collected and prepared by the student.

PRO CE DURE

1. Fill a graduated test tube (0755) to line 6 with *Phosphorus

Extracting Solution (5704).

2. Use 0.5g spoon (0698) to add two measures of the soil sample to the
test tube. Cap and gently shake for one minute.

3. Without removing the cap, allow the tube to stand undisturbed until
the soil particles settle, leaving a clear liquid layer above the soil.

4. Use the pipet (0364) to fill a second test tube to line 3 with the clear
extract. To accomplish this, squeeze the bulb of the pipet before
inserting into the test tube containing the soil and extracting
solution. This prevents agitation of the clear extract. After inserting
release the pressure on the bulb and “draw-up” a portion of the clear
extract. Continue to transfer clear extract in this manner until the
level of solution is even with line 3 of the second tube.

5. Add 6 drops of the *Phosphorus Indicator Reagent (5705) to second
test tube. Cap and shake to mix.

6. Add one *Phosphorus Tablet (5706). Cap and shake until tablet
disintegrates.

7. What color appeared? Match color of the reaction to the colors
printed on the Soil Phosphorus Color Chart (1372). Is the
phosphorus level High? Medium? or Low? Record the result on a Soil
Analysis Report Form (1582).

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Chal lenges

PO Box 329 • Chestertown • Mary land • 21620 • USA

800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax: [phone]

Visit us on the web at www.lamotte.com

®

10.04