LaMotte SMARTLink3 User Manual

1910-MN

2.13.12

CONTENTS

 GENERAL INFORMATION

Packaging & Delivery ...................................................................................... 3

General Precautions ...................................................................................... 3

Safety Precautions ......................................................................................... 3

Limits of Liability ............................................................................................. 4

Warranty ......................................................................................................... 4

Register Your Meter ......................................................................................... 4

Speciﬁ cations ................................................................................................ 5

Statistical and Technical Deﬁ nitions ............................................................... 6

Contents and Accessories ............................................................................. 7

EPA Compliance ............................................................................................ 8

CE Compliance .............................................................................................. 8

IP 67 Certiﬁ cation ............................................................................................ 8

 CHEMICAL TESTING

Water Sampling for Chemical Analysis .......................................................... 9

Filtration ....................................................................................................... 10

An Introduction to Colorimetric Analysis ..................................................... 11

Reagent Blank ............................................................................................. 12

Colorimeter Tubes & Chamber ................................................................... 12

Meter Care .................................................................................................... 12

Selecting an Appropriate Wavelength ........................................................ 12

Calibration .................................................................................................... 13

Calibration Curves ....................................................................................... 13

Standard Additions ...................................................................................... 16

Sample Dilution & Volumetric Measurements ............................................ 17

Interferences ................................................................................................ 18

Stray Light Interference ............................................................................... 18

 OPERATION OF THE SMART 3 COLORIMETER

Overview ...................................................................................................... 20

Components ................................................................................................ 20

 GENERAL OPERATING PROCEDURES

The Keypad ................................................................................................. 21

Sample Holders ............................................................................................ 21

The Display & the Menus ............................................................................ 22

Looping Menus ............................................................................................. 24

 TESTING

Testing Menu ............................................................................................... 25

Test Sequences ........................................................................................... 26

General Testing Procedures ........................................................................ 26

Testing With LaMotte Pre-Programmed Tests ............................................. 27

Calibrating LaMotte Pre-Progammed Tests ................................................. 29

Measuring in the Absorbance Mode ............................................................ 34

 EDITING MENU

Editing a Sequence ..................................................................................... 36

Adding a Test ............................................................................................... 38

Deleting a Test ............................................................................................... 40

Edit User Tests ............................................................................................. 42

Naming the Test ........................................................................................... 44

Selecting the Vial and Wavelength ............................................................. 47

Entering a Two Point Calibration ................................................................. 48

Entering a Multiple Point Calibration ............................................................. 52

Selecting the Numerical Format of the Result ............................................. 55

Selecting Units of Concentration ................................................................. 56

Setting the Clock .......................................................................................... 57

Logging Data ................................................................................................ 58

Factory Setup ............................................................................................... 59

Setting the Power Save Function ................................................................. 59

Setting the Backlight Time ............................................................................ 61

Selecting a Language ................................................................................... 62

 COMPUTER CONNECTION

PC Link .......................................................................................................... 64

Output .......................................................................................................... 64

Computer Connection .................................................................................. 64

SMARTLink 3 ................................................................................................. 64

 BATTERY

Battery/AC Operation .................................................................................... 65

Battery Replacement ..................................................................................... 65

 MAINTENANCE

Cleaning ...................................................................................................... 66

Repairs .......................................................................................................... 66

Meter Disposal .............................................................................................. 66

 TROUBLESHOOTING

Error Messages ............................................................................................ 67

Troubleshooting Guide ................................................................................. 67

 SMART3 COLORIMETER TEST PROCEDURES  APPENDIX

GENERAL INFORMATION

 PACKAGING & DELIVERY

Experienced packaging personnel at LaMotte Company assure adequate protection against normal hazards encountered in transportation of shipments. After the product leaves the manufacturer, all responsibility for its safe delivery is assured by the transportation company. Damage claims must be ﬁ led immediately with the transportation company to receive compensation for damaged goods.

Should it be necessary to return the instrument for repair or servicing, pack instrument carefully in a suitable container with adequate packing material. A return authorization number must be obtained from LaMotte Company by calling 1-800-344-3100 or emailing tech@lamotte.com. Attach a letter with the authorization number to the shipping carton which describes the kind of trouble experienced. This valuable information will enable the service department to make the required repairs more efﬁ ciently.

 GENERAL PRECAUTIONS

Before attempting to set up or operate this instrument it is important to read the instruction manual. Failure to do so could result in personal injury or damage to the equipment.

The SMART3 Colorimeter should not be stored or used in a wet or corrosive environment. Care should be taken to prevent water or reagent chemicals from wet colorimeter tubes from entering the colorimeter chamber.

NEVER PUT WET TUBES IN COLORIMETER.

 SAFETY PRECAUTIONS

Read the labels on all LaMotte reagent containers prior to use. Some containers include precautionary notices and ﬁ rst aid information. Certain reagents are considered hazardous substances and are designated with a * in the instruction manual. Material Safety Data Sheets (MSDS) can be found at www.lamotte. com. Read the MSDS before using these reagents. Additional emergency information for all LaMotte reagents is available 24 hours a day from the Poison Control Center listed in the front of the phone book or by contacting the 24 hour emergency line for ChemTel 1-800-255-3924 (USA, Canada, Puerto Rico); locations outside the North American Continent 813-248-0585 (call collect). Be prepared to supply the name and four-digit LaMotte code number found on the container label or at the top of the MSDS or in the contents list of the procedure. LaMotte reagents are registered with a computerized poison control information system available to all local poison control centers.

Keep equipment and reagent chemicals out of the reach of young children.

SMART3 Colorimeter 11.10 3

 LIMITS OF LIABILITY

Under no circumstances shall LaMotte Company be liable for loss of life, property, proﬁ ts, or other damages incurred through the use or misuse of its products.

 WARRANTY

LaMotte Company warrants this instrument to be free of defects in parts and workmanship for 2 years from the date of shipment. If it should become necessary to return the instrument for service during or beyond the warranty period, contact our Technical Service Department at 1-800-344-3100 or tech@lamotte.com for a return authorization number or visit www.lamotte. com for troubleshooting help. The sender is responsible for shipping charges, freight, insurance and proper packaging to prevent damage in transit. This warranty does not apply to defects resulting from action of the user such as misuse, improper wiring, operation outside of speciﬁ cation, improper maintenance or repair, or unauthorized modiﬁ cation. LaMotte Company speciﬁ cally disclaims any implied warranties or merchantability or ﬁ tness for a speciﬁ c purpose and will not be liable for any direct, indirect, incidental or consequential damages. LaMotte Company’s total liability is limited to repair or replacement of the product. The warranty set forth above is inclusive and no other warranty, whether written or oral, is expressed or implied.

 REGISTER YOUR METER

To register your meter with the LaMotte Service Department, go to www.lamotte.com and choose SUPPORT on the top navigation bar.

4 SMART3 Colorimeter 02.12

 SPECIFICATIONS

INSTRUMENT TYPE: Colorimeter

Readout 160 x 100 backlit LCD, 20 x 6 line graphical display

Wavelengths 428 nm, 525 nm, 568 nm, 635 nm

Wavelength Accuracy ±2% FS

Readable Resolution Determined by reagent system

Wavelength Bandwidth 10 nm typical

Photometric Range –2 to +2 AU

Photometric Precision ± 0.001 AU at 1.0 AU

Photometric Accuracy ±0.005 AU at 1.0 AU

Sample Chamber Accepts 25 mm diameter ﬂ at-bottomed test tubes, 10

mm square cuvettes, 16 mm COD test tubes

Light Sources 4 LEDs

Detectors 4 silicon photodiodes

Modes Pre-programmed tests, absorbance, %T

Pre-Programmed Tests YES, with automatic wavelength selection

User Deﬁ ned Tests Up to 25 user tests can be input

Languages English, Spanish, French, Portuguese, Italian,

Chinese, Japanese

USB Port Mini B

Power Requirements USB wall adapter, USB computer connection or

lithium ion rechargeable battery

Battery Charge Life: Approximately 380 tests with backlight

on to 1000 tests with backlight off. (Signal averaging disabled). Battery Life: Approximately 500 charges.

Electrical Rating Provided on nameplate label

Data Logger 500 test results stored for download to a PC

Waterproof IP67 with USB port plug in place

Dimensions (LxWxH) 3.5 x 7.5 x 2.5 inches, 8.84 x 19.05 x 6.35 cm

Weight 13 oz, 362 g (meter only)

SMART3 Colorimeter 02.12 5

 STATISTICAL & TECHNICAL DEFINITIONS RELATED TO

PRODUCT SPECIFICATIONS

Method Detection Limit (MDL): “The method detection limit (MDL) is deﬁ ned as the minimum concentration of a substance that can be measured and reported with 99% conﬁ dence that the analyte concentration is greater than zero and is determined from analysis of a sample in a given matrix containing the analyte.”1 Note that, “As Dr. William Horwitz once stated, ‘In almost all cases when dealing with a limit of detection or limit of determination, the primary purpose of determining that limit is to stay away from it.’”

2

Accuracy: Accuracy is the nearness of a measurement to the accepted or true value.3 The accuracy can be expressed as a range, about the true value, in which a measurement occurs (i.e. ±0.5 ppm). It can also be expressed as the % recovery of a known amount of analyte in a determination of the analyte (i.e.

103.5 %).

Resolution: Resolution is the smallest discernible difference between any two measurements that can be made.4 For meters this is usually how many decimal places are displayed. (i.e. 0.01). Note that the resolution many change with concentration or range. In some cases the resolution may be less than the smallest interval, if it is possible to make a reading that falls between calibration marks. A word of caution, that resolution has very little relationship to accuracy or precision. The resolution will always be less than the accuracy or precision but it is not a statistical measure of how well a method of analysis works. The resolution can be very, very good and the accuracy and precision can be very bad! This is not a useful measure of the performance of a test method.

Repeatability: Repeatability is the within-run precision.5 A run is a single data set, from set up to clean up. Generally, one run occurs on one day. However, for meter calibrations, a single calibration is considered a single run or data set, even though it may take 2 or 3 days.

Reproducibility: Reproducibility is the between-run precision.

6

Detection Limit (DL): The detection limit (DL) for the 2020we/wi is deﬁ ned as the minimum value or concentration that can be determined by the meter, which is greater than zero, independent of matrix, glassware, and other sample handling sources of error. It is the detection limit for the optical system of the meter.

1

CFR 40, part 136, appendix B

2

Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta,

American Laboratory, Sept 2003, P. 31.

3

Skoog, D.A., West, D. M., Fundamental of Analytical Chemistry, 2nd ed., Holt

Rinehart and Winston, Inc, 1969, p. 26.

4

Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta,

American Laboratory, Sept 2003, P. 34.

6 SMART3 Colorimeter 11.10

5

Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of

Quantitative Chemical Analysis, 5th ed., Longman Scientiﬁ c & Technical, 1989, p.

130.

6

Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative Chemical Analysis, 5th ed., Longman Scientiﬁ c & Technical, 1989, p. 130

 CONTENTS AND ACCESSORIES

CONTENTS

SMART3 Colorimeter

Test Tubes, with Caps

COD/UDV Adapter

USB Wall Adapter

USB Cable

SMART3 Colorimeter Quick Start Guide

SMART3 Colorimeter Manual

ACCESSORIES

Test Tubes, with Caps Code 0290-6

Replacement Chamber Code 3-0038

USB Cable Code 1720

USB Wall Adapter Code 1721

COD/UDV Adapter Code 1724

Car Charger Code 5-0132

SMARTLink3 Program (CD) Code 1901-CD

Small Field Carrying Case

Code 1910-GCS150

(37.5 27.5 x 13.75 cm)

Large Field Carrying Case

Code 1910-GCS440

(45 x 32.5 x 20 cm)

SMART3 Colorimeter 02.12 7

 EPA COMPLIANCE

The SMART3 Colorimeter is an EPA-Accepted instrument. EPA-Accepted means that the instrument meets the requirements for instrumentation as found in test procedures that are approved for the National Primary Drinking Water Regulations (NPDWR) or National Pollutant Discharge Elimination System (NPDES) compliance monitoring programs. EPA-Accepted instruments may be used with approved test procedures without additional approval.

 CE COMPLIANCE

The SMART3 Colorimeter has earned the European CE Mark of Compliance for electromagnetic compatibility and safety. The Declaration of Conformity for the SMART3 colorimeter is available at www.lamotte.com.

 IP67 CERTIFICATION

The SMART3 meets IP67 standards for protection against dust and immersion only when the USB port plug is in place. Documentation is available at www. lamotte.com.

8 SMART3 Colorimeter 11.10

CHEMICAL TESTING

 WATER SAMPLING FOR CHEMICAL ANALYSIS

Taking Representative Samples

The underlying factor to be considered for any type of water sampling is whether or not the sample is truly representative of the source. To properly collect a representative sample:

• Sample as frequently as possible.

• Collect a large sample or at least enough to conduct whatever tests are necessary.

• Make a composite sample for the same sampling area.

• Handle the sample in such a way as to prevent deterioration or contamination before the analysis is performed.

• Perform analysis for dissolved gases such as dissolved oxygen, carbon dioxide, and hydrogen sulﬁ de immediately at the site of sampling. Samples for testing these factors, as well as samples for pH, cannot be stored for later examination.

• Make a list of conditions or observations which may affect the sample. Other considerations for taking representative samples are dependent upon the source of the sample. Taking samples from surface waters involves different considerations than taking samples from impounded and sub-surface waters.

Sampling of Open Water Systems

Surface waters, such as those found in streams and rivers, are usually well mixed. The sample should be taken downstream from any tributary, industrial or sewage pollution source. For comparison purposes samples may be taken upstream and at the source of the pollution before mixing.

In ponds, lakes, and reservoirs with restricted ﬂ ow, it is necessary to collect a number of samples in a cross section of the body of water, and where possible composite samples should be made to ensure representative samples.

To collect samples from surface waters, select a suitable plastic container with a tight ﬁ tting screw cap. Rinse the container several times with the sample to be tested, then immerse the container below the surface until it is ﬁ lled to overﬂ owing and replace the cap. If the sample is not to be tested immediately, pour a small part of the sample out and reseal. This will allow for any expansion. Any condition which might affect the sample should be listed.

Sub-surface sampling is required to obtain a vertical proﬁ le of streams, lakes, ponds, and reservoirs at speciﬁ c depths. This type of sampling requires more sophisticated sampling equipment.

For dissolved oxygen studies, or for tests requiring small sample sizes, a Water

SMART3 Colorimeter 11.10 9

Sampler (LaMotte Code 1060) will serve as a subsurface or in-depth sampler. This weighted device is lowered to the sampling depth and allowed to rest at this depth for a few minutes. The water percolates into the sample chamber displacing the air which bubbles to the surface. When the bubbles cease to rise, the device has ﬂ ushed itself approximately ﬁ ve times and it may be raised to the surface for examination. The inner chamber of the sampling device is lifted out and portions of the water sample are carefully dispensed for subsequent chemical analysis.

A Snap-Plunger Water Sampler (LaMotte Code 1077) is another “in-depth” sampling device which is designed to collect large samples which can be used for a multitude of tests. Basically, this collection apparatus is a hollow cylinder with a spring loaded plunger attached to each end. The device is cocked above the surface of the water and lowered to the desired depth. A weighted messenger is sent down the calibrated line to trip the closing mechanism and the plungers seal the sample from mixing with intermediate layers as it is brought to the surface. A special drain outlet is provided to draw off samples for chemical analysis.

Sampling of Closed System

To obtain representative samples from conﬁ ned water systems, such as pipe lines, tanks, vats, ﬁ lters, water softeners, evaporators and condensers, different considerations are required because of chemical changes which occur between the inlet and outlet water. One must have a basic understanding of the type of chemical changes which occur for the type of equipment used. Also, consideration should be given to the rate of passage and retaining time for the process water.

Temperature changes play an important part in deciding exactly what test should be performed. Process water should be allowed to come to room temperature, 20–25°C, before conducting any tests.

When drawing off samples from an outlet pipe such as a tap, allow sample to run for several minutes, rinsing the container several times before taking the ﬁ nal sample. Avoid splashing and introduction of any contaminating material.

 FILTRATION

When testing natural waters that contain signiﬁ cant turbidity due to suspended solids and algae, ﬁ ltration is an option. Reagent systems, whether EPA, Standard Methods, LaMotte or any others, will generally only determine dissolved constituents. Both EPA and Standard Methods suggest ﬁ ltration through a 0.45 micron ﬁ lter membrane, to remove turbidity, for the determination of dissolved constituents.** To test for total constituents, organically bound and suspended or colloidal materials, a rigorous high temperature acid digestion is necessary.

**LaMotte offers a ﬁ ltering apparatus: syringe assembly (Code 1050) and membrane ﬁ lters, 0.45 micron, (Code 1103).

10 SMART3 Colorimeter 11.10

 AN INTRODUCTION TO COLORIMETRIC ANALYSIS

Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must ﬁ nd a way to “see” them. The SMART3 Colorimeter can be used to measure any test substance that is itself colored or can be reacted to produce a color. In fact a simple deﬁ nition of colorimetry is “the measurement of color” and a colorimetric method is “any technique used to evaluate an unknown color in reference to known colors”. In a colorimetric chemical test the intensity of the color from the reaction must be proportional to the concentration of the substance being tested. Some reactions have limitations or variances inherent to them that may give misleading results. Many such interferences are discussed with each particular test instruction. In the most basic colorimetric method the reacted test sample is visually compared to a known color standard. However, accurate and reproducible results are limited by the eyesight of the analyst, inconsistencies in the light sources, and the fading of color standards.

To avoid these sources of error, a colorimeter can be used to photoelectrically measure the amount of colored light absorbed by a colored sample in reference to a colorless sample (blank).

White light is made up of many different colors or wavelengths of light. A colored sample typically absorbs only one color or one band of wavelengths from the white light. Only a small difference would be measured between white light before it passes through a colored sample versus after it passes through a colored sample. The reason for this is that the one color absorbed by the sample is only a small portion of the total amount of light passing through the sample. However, if we could select only that one color or band of wavelengths of light to which the test sample is most sensitive, we would see a large difference between the light before it passes through the sample and after it passes through the sample.

The SMART3 Colorimeter passes one of four colored light beams through one of four optical ﬁ lters which transmits only one particular color or band of wavelengths of light to the photodectector where it is measured. The difference in the amount of colored light transmitted by a colored sample is a measurement of the amount of colored light absorbed by the sample. In most colorimetric tests the amount of colored light absorbed is directly proportional to the concentration of the test factor producing the color and the path length through the sample. However, for some tests the amount of colored light absorbed is inversely proportional to the concentration.

The choice of the correct wavelength for testing is important. It is interesting to note that the wavelength that gives the most sensitivity (lower detection limit) for a test factor is the complementary color of the test sample. For example the Nitrate-Nitrogen test produces a pink color proportional to the nitrate-nitrogen concentration in the sample (the greater the nitrate-nitrogen concentration, the darker the pink color). A wavelength in the green region should be selected to analyze this sample since a pinkish-red solution absorbs mostly green light.

SMART3 Colorimeter 11.10 11

 REAGENT BLANK

Some tests will provide greater accuracy if a reagent blank is determined to compensate for any color or turbidity resulting from the reagents themselves. A reagent blank is performed by running the test procedure on demineralized or deionized water. Use sample water to SCAN BLANK. Insert the reacted reagent blank in the colorimeter chamber and select SCAN SAMPLE. Note result of reagent blank. Perform the tests on the sample water as described. Subtract results of reagent blank from all subsequent test results. NOTE: Some tests require a reagent blank to be used to SCAN BLANK.

 COLORIMETER TUBES AND CHAMBER

Colorimeter tubes and colorimeter chambers which have been scratched through excessive use should be discarded and replaced with new ones. Dirty tubes should be cleaned on both the inside and outside. Fingerprints on the exterior of the tubes can cause excessive light scattering and result in errors. Handle the tubes carefully, making sure the bottom half of the tube is not handled.

LaMotte Company makes every effort to provide high quality colorimeter tubes. However, wall thicknesses and diameter of tubes may still vary slightly. This may lead to slight variations in results (e.g. if a tube is turned while in the sample chamber, the reading will likely change slightly). To eliminate this error put the tubes into the sample chamber with the same orientation every time.

The tubes that are included with the colorimeter have an index mark to facilitate this. If possible, use the same tube to SCAN BLANK and SCAN SAMPLE.

 METER CARE

The optical system of the SMART3 must be kept clean and dry for optimal performance. Dry the colorimeter tubes before placing them in the chamber to avoid introducing moisture. For best results store the instrument in a area that is dry and free from aggressive chemical vapors.

 SELECTING AN APPROPRIATE WAVELENGTH

The most appropriate wavelength to use when creating a calibration curve is usually the one which gives the greatest change from the lowest reacted standard concentration to the highest reacted standard concentration. However, the absorbance of the highest reacted standard concentration should never be greater than 2.0 absorbance units. Scan the lowest and highest reacted standards at different wavelengths using the absorbance mode to ﬁ nd the wavelength which gives the greatest change in absorbance without exceeding

2.0 absorbance units. Use this wavelength to create a calibration curve.

12 SMART3 Colorimeter 02.12

Below is a list of suggested wavelengths for the color of the reacted samples. Use these as a starting point.

Sample Color Wavelength Range

Yellow 428

Pink 525

Red 568

Green and Blue 635

 CALIBRATION

As with all pre-calibrated meters, it is highly recommended, even if not required by regulations, that the user periodically verify the performance of the meter by running standards with a predetermined concentration. Results outside of speciﬁ cation are an indication that the meter needs to be adjusted. This can be done following the user calibration described on page 28. If the user calibration fails to properly adjust the meter then the meter should be returned to LaMotte Company for recalibration. (See page 65).

 CALIBRATION CURVES

The SMART3 Colorimeter contains tests for the LaMotte reagent systems. The ﬁ rst step in using a non-LaMotte reagent system with your SMART3 Colorimeter is to create a calibration curve for the reagent system. To create a calibration curve, prepare standard solutions of the test factor and use the reagent system to test the standard solutions with the SMART3 Colorimeter. Select a wavelength for the test as described above.

Plot the results (in ABS or %Transmittance) versus concentration to create a calibration curve. The calibration curve may then be used to identify the concentration of an unknown sample by testing the unknown, reading Absorbance or %T, and ﬁ nding the corresponding concentration from the curve. The linear range of the reagent system can be determined and this information can be used to input a User Test into the SMART3 Colorimeter (see Edit User Tests, page 41).

PROCEDURE

Prepare 5 or 6 standard solutions of the factor being tested. The concentration of these standards should be evenly distributed throughout the range of the reagent system, and should include a 0 ppm standard (distilled water). For instance, the solutions could measure 0, 10%, 30%, 50%, 70%, and 90% of the system’s maximum range.

1. Turn on the SMART3 Colorimeter. Select the appropriate wavelength from the absorbance mode. Be sure to select the appropriate wavelength for the color produced by the reagent system.

2. Use the unreacted 0 ppm standard to standardize the colorimeter by using it

SMART3 Colorimeter 11.10 13

to scan blank.

3. Following the individual reagent system instructions, react each standard solution beginning with 0 ppm. Continue with standards in increasing concentration. Record the reading and the standard solution concentration on a chart. Readings can be recorded as percent transmittance (%T) or absorbance (A).

4. Plot results on graph paper or computer using any available plotting program. If results are as %T versus concentration, semilog graph paper must be used. Plot the standard solution concentrations on the horizontal, linear axis, and the %T on the vertical, logarithmic axis. If results are as absorbance versus standard solution concentration, simple linear graph paper can be used. Plot the standard solution concentration on the horizontal axis, and the absorbance on the vertical axis.

5. After plotting the results, draw a line, or curve, of best ﬁ t through the plotted points. The best ﬁ t may not connect the points. There should be approximately an equal number of points above the curve as below the curve. Some reagent systems will produce a straight line, while others produce a curve. Many computer spreadsheet programs can produce the curve of best ﬁ t by regression analysis of the standard solution data.

NOTE: Only reagent systems which produce a straight line can be used for a User Test.

A sample of each type of graph appears below:

14 SMART3 Colorimeter 11.10

PREPARING DILUTE STANDARD SOLUTIONS

Standard solutions should be prepared to create a calibration curve. Standard solutions can be prepared by diluting a known concentrated standard by speciﬁ ed amounts. A chart or computer spreadsheet can be created to determine the proper dilutions. Use volumetric ﬂ asks and volumetric pipets for all dilutions.

1. In Column A – Record the maximum concentration of test as determined by the range and path length.

2. In Column B – Record the percent of the maximum concentration the standard solution will be.

3. In Column C – Calculate the ﬁ nal concentration of the diluted standard solutions by multiplying the maximum concentration (In Column A) by the %

of maximum concentration divided by 100. (C = A x

4. In Column D – Record the ﬁ nal volume of the diluted sample (i.e. volume of volumetric ﬂ ask).

5. In Column E – Record the concentration of the original standard.

6. In Column F – Calculate the milliliters of original standard required (F = (C x

D

/E)).

B

/

).

100

A sample chart appears below:

A B C =

Maximum

concentration

of test

% of Maximum

concentration

A x B/

concentration

of Diluted

Standard

Final

100

D E F =

Volume of

Standard

Concentration

of Original

Standard

C x D/E

mL of

Original Standard Required

10.0 ppm 90 9.0 ppm 100 mL 1000 ppm 0.90 mL

10.0 ppm 70 7.0 ppm 100 mL 1000 ppm 0.70 mL

10.0 ppm 50 5.0 ppm 100 mL 1000 ppm 0.50 mL

10.0 ppm 30 3.0 ppm 100 mL 1000 ppm 0.30 mL

10.0 ppm 10 1.0 ppm 100 mL 1000 ppm 0.10 mL

10.0 ppm 0 0 ppm 100 mL 1000 ppm 0 mL

SMART3 Colorimeter 11.10 15

 STANDARD ADDITIONS

A common method to check the accuracy and precision of a test is by standard additions. In this method a sample is tested to determine the concentration of the test substance. A second sample is then “spiked” by the addition of a known quantity of the test substance. The second sample is then tested. The determined concentration of the spiked sample should equal the concentration of the ﬁ rst plus the amount added with the spike. The procedure can be repeated with larger and larger “spikes.” If the determined concentrations do not equal the concentration of the sample plus that added with the “spike”, then an interference may exist.

For example, a 10.0 mL water sample was determined to contain 0.3 ppm iron. To a second 10.0 mL sample, 0.1 mL of 50 ppm iron standard was added. The concentration of iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50 ppm. The concentration of iron determined in the spiked sample should be 0.3 + 0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is negligible).

LaMotte offers a line of calibration standards which can be used to generate calibration curves and perform standard additions.

16 SMART3 Colorimeter 11.10

 SAMPLE DILUTION TECHNIQUES & VOLUMETRIC

MEASUREMENTS

If a test result using the SMART3 Colorimeter gives an over range message then the the sample must be diluted. The test should be repeated on the diluted sample to obtain a reading which is in the concentration range for the test. (Note: This is not true for colorimetric determination of pH.)

Example:

Measure 5 mL of the water sample into a graduated cylinder. Add demineralized water until the cylinder is ﬁ lled to the 10 mL line. The sample has been diluted by one-half, and the dilution factor is therefore 2. Perform the test procedure, then multiply the resulting concentration by 2 to obtain the test result.

The following table gives quick reference guidelines on dilutions of various proportions. All dilutions are based on a 10 mL volume, so several dilutions will require small volumes of the water sample. Graduated pipets should be used for all dilutions.

Deionized Water to

Size of Sample

10 mL 0 mL 1

5 mL 5 mL 2

2.5 mL 7.5 mL 4

1 mL 9 mL 10

0.5 mL 9.5 mL 20

Bring Volume to 10 mL Multiplication Factor

If the above glassware is not available, dilutions can be made with the colorimeter tube. Fill the tube to the 10 mL line with the sample then transfer it to another container. Add 10 mL volumes of demineralized water to the container and mix. Transfer back 10 mL of the diluted sample to the tube and follow the test procedure. Continue diluting and testing until a reading, which is in the concentration range for the test, is obtained. Be sure to multiply the concentration found by the dilution factor (the number of total 10 mL volumes used).

Example:

10 mL of sample is diluted with three 10 mL volumes of demineralized water; the dilution factor is four.

SMART3 Colorimeter 11.10 17

 INTERFERENCES

LaMotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test. Be aware of possible interferences in the water being tested.

The reagent systems also contain buffers to adjust the water sample to the ideal pH for the reaction. It is possible that the buffer capacity of the water sample may exceed the buffer capacity of the reagent system and the ideal pH will not be obtained. If this is suspected, measure the pH of a reacted distilled water reagent blank using a pH meter. This is the ideal pH for the test. Measure the pH of a reacted water sample using the pH meter. If the pH is signiﬁ cantly different from the ideal value, the pH of the sample should be adjusted before testing.

Interferences due to high concentration of the substance being tested, can be overcome by sample dilution (see page 16)

 STRAY LIGHT INTERFERENCE

When scanning samples in 16 mm tubes, such as COD, the sample chamber lid can not be closed. The COD adapter minimizes stray light. To further reduce stray light interference, do not scan sample in direct sunlight.

18 SMART3 Colorimeter 11.10

OPERATION OF THE SMART3 COLORIMETER

 OVERVIEW

The SMART3 is a portable, microprocessor controlled, direct reading colorimeter. It has a graphical liquid crystal display and 6 button keypad. These allow the user to select options from the menu driven software, to directly read test results or to review stored results of previous tests in the data logger. The menus can be displayed in seven different languages.

The test library consists of over 80 LaMotte tests and 25 “User Tests”. The LaMotte tests are precalibrated for LaMotte reagent systems.The colorimeter displays the result of these tests directly in units of concentration. The 25 “User Tests” may be used to enter additional calibrations. All of these tests may be arranged in any of 3 sequences. These sequences can be modiﬁ ed a limitless number of times to meet changing testing needs.

The optics feature 4 different colored LEDs. Each LED has a corresponding silicon photoiode with an integrated interference ﬁ lter. The interference ﬁ lters select a narrow band of light from the corresponding LED for the colorimetric measurements. The microporcessor automatically selects the correct LED/ photodiode combination for the test.

A USB wall adapter, USB computer connection or lithium battery powers the SMART3.

A USB port on the back of the meter allows an interface of the meter with a Windows-based computer for real-time data acquisition and data storage using a PC. The SMART3 may be interfaced with any Windows-based computer by using the LaMotte SMARTLink3 Program.

SMART3 Colorimeter 11.10 19

 COMPONENTS

Figure 1 shows a diagram of the SMART3 Colorimeter and its components.

20 SMART3 Colorimeter 11.10

GENERAL OPERATING PROCEDURES

The operation of the SMART3 Colorimeter is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the colorimeter to perform, such as, scan blank, scan sample, and edit test sequences. The keypad is used to make menu selections which are viewed in the display. There are three selections accessible from the Main Menu: Testing Menu, Editing Menu and Run PC Link.

 THE KEYPAD

The keypad has 6 buttons which are used to perform speciﬁ c tasks.

This button will scroll up through a list of menu selections.

ENTER

The button is used to select choices in a menu viewed in the display.

This button controls the backlight on the display.

This button will scroll down through a list of menu selections.

EXIT

This button exits to the previous menu.

This button turns the meter on or off.

ENTER

EXIT

 SAMPLE HOLDERS

The sample chamber is designed for 25 mm round tubes. An adapter to hold 16 mm COD tubes and 1 cm square UDV cuvettes is included.

Position the COD/UDV Adapter (Code 1724) so that the notches in the adapter ﬁ t around the posts on the chamber. Turn the adapter counterclockwise until the arrows are at the top and bottom of the chamber and the adapter is locked into place. Turn the adapter clockwise to unlock the adapter and remove it from the chamber.

SMART3 Colorimeter 11.10 21

 THE DISPLAY & THE MENUS

The display allows menu selections to be viewed and selected. These selections instruct the SMART3 to perform speciﬁ c tasks. The menus are viewed in the display using two general formats that are followed from one menu to the next. Each menu is a list of choices or selections.

The display has a header line at the top and a footer line at the bottom. The header displays the title of the current menu. The footer line displays the time and the date, the data logger status and the battery status. The menu selection window is in the middle of the display between the header and the footer.

The menu selection window displays information in two general formats. In the ﬁ rst format only menu selections are displayed. Up to 4 lines of menu selections may be displayed. If more selections are available they can be viewed by pressing the arrow buttons the menu selection window. Think of the menu selections as a vertical list in the display that moves up or down each time an arrow button is pressed. Some menus in the SMART3 are looping menus. The top and bottom menu choices are connected in a loop. Scrolling down past the bottom of the menu will lead to the top of the menu. Scrolling up past the top of the menu will lead to the bottom of the menu.

Main Window Selection First Choice

to scroll the other menu selections into

Header Menu Title

Second Choice

Third Choice

Another

Footer

12:00:00 001/500

And Another

And So On

A black bar will indicate the menu choice. As the menu is scrolled through, the black bar will highlight different menu choices. Pressing the

ENTER

button will

select the menu choice that is indicated by the black bar.

In the second format the menu choice window takes advantage of the graphical capabilities of the display. Large format graphic information, such as test results or error messages or the LaMotte logo is displayed. The top two lines of the display are used to display information in a large, easy to read format. The menus work in the same way as previously described but two lines of the menu are visible at the bottom of the display.

22 SMART3 Colorimeter 11.10

Header Menu Title

Message or Result Window

Result or Message

Another

Main Window Selection And Another

Footer

12:00:00 001/500

And So On

Last Choice

As described previously, the current menu and a return to the previous menu. This allows a rapid exit from an inner menu to the main menu by repeatedly pushing the

at any time will turn the SMART3 off.

The display may show the following messages:

Battery Status

More choices are available and can be viewed by scrolling up and/or down through the display.

Header Identiﬁ es the current menu and information on units

and reagent systems if applicable.

Footer In the data logging mode the number of the data point

is displayed and the total number of data points in the memory will be shown. The footer also shows current time and battery status

EXIT

button allows an exit or escape from the

EXIT

button. Pushing

SMART3 Colorimeter 11.10 23

 LOOPING MENUS

Long menus, such as All Tests, incorporate a looping feature which allows the user to quickly reach the last choice in the menu from the ﬁ rst choice. In a looping menu the last choices in the menu are above the ﬁ rst choice and scrolling upward moves through the menu in reverse order. Scrolling downward moves through the menu from ﬁ rst choice to last but the menu starts over following the last choice. So all menu choices can be reached by scrolling in either direction. The diagrams below demonstrate a looping menu.

AND SO ON AND SO ON AND SO ON

: : : : : : : : :

THIRD TO LAST LAST CHOICE LAST CHOICE

SECOND TO LAST AND SO ON AND SO ON

LAST CHOICE : : : : : :

TESTING MENU TESTING MENU TESTING MENU

FIRST CHOICE : : : : : :

SECOND CHOICE THIRD TO LAST THIRD TO LAST

THIRD CHOICE SECOND TO LAST SECOND TO LAST

ANOTHER LAST CHOICE LAST CHOICE

12:00:00 001/500 12:00:00 001/500 12:00:00 001/500

AND ANOTHER FIRST CHOICE FIRST CHOICE

AND SO ON SECOND CHOICE SECOND CHOICE

: : : THIRD CHOICE THIRD CHOICE

: : : ANOTHER ANOTHER

LAST CHOICE AND ANOTHER AND ANOTHER

24 SMART3 Colorimeter 11.10

TESTING

 TESTING MENU

The Testing Menu is used to run all LaMotte pre-programmed tests, User Tests and Absorbance tests at one of four wavelengths. Testing from any of three sequences can also be done.

1. Press and brieﬂ y hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

ENTER

to select Testing

Menu.

Testing Menu

Editing Menu

Run PC Link

12:00:00 001/500

All Tests Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

Main Menu

Testing Menu

Testing

3. Press or to scroll to desired option. All Tests contains all of the available pre-programmed tests. The three sequences have user selected tests. Absorbance has %T/ABS tests.

4. Press

ENTER

to select the

option.

All Tests Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

001 Alkalinity UDV

Testing Menu

All Tests

002 Aluminum

003 Ammonia-N LRF

004 Ammonia-N LRS

12:00:00 001/500

SMART3 Colorimeter 11.10 25

 TEST SEQUENCES

Sequence 1, Sequence 2, And Sequence 3 are alterable sequences. They may be edited using the Editing Menu. Any of the LaMotte pre-programmed tests or User Tests may be placed in these sequences in whatever testing order that is preferred. Some examples of typical sequences are given below.

Testing

Sequence 1 Sequence 2 Sequence 3

015 Chlorine F UDV 002 Aluminum 003 Ammonia-N LRF

079 Phosphate HR 035 Cyanuric Acid 032 Cu UDV

009 Benzotriazole 053 Iron Phenanthro 064 Nitrate-N LR

076 pH UDV 055 Manganese LR 067 Nitrite-N LR

12:00:00 001/500 12:00:00 001/500 12:00:00 001/500

061 Molybdenum HR 064 Nitrate-N LR 074 pH PR

086 Silica HR 067 Nitrite-N LR 078 Phosphate LR

045 Hydrazine 077 Phenol 085 Silica LR

032 Cu UDV 078 Phosphate LR

051 Iron Bipyridyl 090 Sulﬁ de LR

These alterable sequences allow a series of tests to be setup that are run frequently. The order of the individual tests in the sequence is determined by the

ENTER

user. After running a test, press

to select the next test in the sequence.

Continue this pattern until the entire sequence has been completed.

All Tests is a ﬁ xed sequence containing the LaMotte pre-programmed tests, User Tests, and Absorbance tests. Modiﬁ cation of the alterable sequences is accomplished through the Editing Menu. This menu is explained in greater detail in Editing Menu (p. 35).

Pressing

EXIT

while in a sequence menu will escape back to the Testing Menu.

Pressing the at any time will turn the colorimeter off.

 GENERAL TESTING PROCEDURES

The following are some step by step examples of how to run tests from the Testing Menu. These test procedures are designed to be used with LaMotte SMART Reagent Systems.

LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available. Pre-programmed calibrations can be added to the SMART3 Colorimeter in the ﬁ eld. A Windows-based computer running a Windows Operating System is required.

Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or email at tech@lamotte.com for a current list of available calibrations and downloading instructions.

26 SMART3 Colorimeter 11.10

 TESTING WITH LaMOTTE PRE-PROGRAMMED TESTS

1. Press and brieﬂ y hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

ENTER

to select Testing

Menu.

3. Press

ENTER

to select All Tests

Menu.

Main Menu

Testing Menu

Editing Menu

Run PC Link

12:00:00 001/500

Testing Menu

All Test Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

All Tests

001 Alkalinity UDV

002 Aluminum

003 Ammonia-N LRF

004 Ammonia-N LRS

12:00:00 001/500

Testing

4. Press or to scroll to the desired test.

001 Alkalinity UDV

All Tests

002 Aluminum

003 Ammonia-N LRF

004 Ammonia-N LRS

12:00:00 001/500

5. Press

ENTER

to select the test.

002 Aluminum

Scan Bank

Scan Sample

12:00:00 001/500

SMART3 Colorimeter 11.10 27

6. Insert the blank into the chamber. Close the lid.Press

ENTER

to scan the blank. The screen wil display Blank Done for about 1 second and then return to the Test Menu.

Testing

002 Aluminum

Scan Blank

Scan Sample

12:00:00 001/500

7. Insert the reacted sample into the chamber. Close the lid. Press the sample. The screen will display READING for about 1 second. The result will appear on the screen.

8. To repeat the test, press to scan the sample again. The last blank scaned is used by the colorimeter for repeated scans. A different blank can be used by pressing or

to scroll to Scan Blank and then scanning another blank. Scroll with or

and make another selection with or Absorbance of the last test can be viewed by choosing %T/Abs. Press

escape to previous menus. NOTE: The menus loop in this screen so either or

will lead to the menu selection needed.

ENTER

EXIT

to scan

. The %T

to

ENTER

002 Aluminum

1.00 ppm

Scan Blank

Scan Sample

12:00:00 001/500

002 Aluminum

1.00 ppm

Scan Bank

Scan Sample

12:00:00 001/500

28 SMART3 Colorimeter 11.10

 CALIBRATING LaMOTTE PRE-PROGRAMMED TESTS

The LaMotte Pre-Programmed Tests have been pre-calibrated. Recalibration of the pre-programmed tests by the user is not possible. However, a procedure to standardize the calibration can be performed to obtain the most accurate readings or to meet regulatory requirements.

The LaMotte Pre-Programmed tests are standardized with one standard solution. To standardize over the full range of the test, the concentration of the standard should be chosen from the high end of the range. Alternatively, if samples do not cover the full range of the test, a standard should be chosen that is close to the concentration of the samples.

The standardization procedure should be followed as often as required by regulations and laws for compliance monitoring.

In the example below, the Aluminum calibration will be standardized.

Prepare a standard solution to be tested. In this example, 0.30 ppm aluminum.

Testing

1. Press and brieﬂ y hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

ENTER

to select Testing

Menu.

3. Press

ENTER

to select All Tests

Menu.

Main Menu

Testing Menu

Editing Menu

Run PC LINK

12:00:00 001/500

Testing Menu

All Test Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

All Tests

001 Alkalinity UDV

002 Aluminum

003 Ammonia-N LRF

004 Ammonia-N LRS

12:00:00 001/500

SMART3 Colorimeter 11.10 29

4. Press or to scroll to the desired test factor.

Testing

All Tests

001 Alkalinity UDV

002 Aluminum

003 Ammonia-N LRF

004 Ammonia-N LRS

12:00:00 001/500

5. Press

ENTER

to select the test.

6. Follow the test procedure in the manual to test the prepared standard. Insert the blank into the chamber. Close the lid. Press

ENTER

to scan the blank. The screen will display Blank Done for about 1 second and then return to the Test Menu.

7. Insert the reacted standard solution into the chamber. Close the lid. Press

ENTER

to scan the sample. The screen will display Reading for about 1 second. The result will appear on the screen.

002 Aluminum

Scan Blank

Scan Sample

12:00:00 001/500

002 Aluminum

Scan Blank

Scan Sample

12:00:00 001/500

002 Aluminum

0.28 ppm

Scan Blank

Scan Sample

12:00:00 001/500

30 SMART3 Colorimeter 11.10

8. The displayed result can now be standardized. Press or to scroll to calibrate.

002 Aluminum

0.28 ppm

%T/Abs

Calibrate

12:00:00 001/500

Testing

9. Press

10. Press or to scroll

11. Press

ENTER

to select Calibrate. A reverse font (dark background with light characters) will appear to indicate that the reading can be adjusted.

to the concentration of the prepared standard, 0.30 in this example. NOTE: A maximum adjustment of 10% is possible. If an adjustment of over 10% is attempted, Overrange will be displayed.

ENTER

to select Calibrate. Two menu choices will be offered, set calibration and factory setting.

002 Aluminum

0.28 ppm

%T/Abs

Calibrate

12:00:00 001/500

002 Aluminum

0.30 ppm

%T/Abs

Calibrate

12:00:00 001/500

002 Aluminum

0.30 ppm

Set Calibration

Factory Setting

12:00:00 001/500

SMART3 Colorimeter 11.10 31

12. Press

ENTER

to select Set

Calibration and save the

calibration. Or press to scroll to Factory Setting. Press Setting to revert to the factory

Testing

ENTER

to select Factory

calibration. The screen will display Storing... for about 1 second and the test menu will appear. The calibration has now been standardized and the meter can be used for testing. The standardization can be removed by repeating the calibration and selecting Factory Setting.

002 Aluminum

Scan Blank

Scan Sample

12:00:00 001/500

32 SMART3 Colorimeter 11.10

 MEASURING IN THE ABSORBANCE MODE

1. Press and brieﬂ y hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

ENTER

to select Testing

Menu.

3. Press or to scroll to Absorbance.

Main Menu

Testing Menu

Editing Menu

Run PC Link

12:00:00 001/500

Testing Menu

All Test Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

Testing Menu

Sequence 1

Sequence 2

Sequence 3

Absorbance

12:00:00 001/500

Testing

4. Press Absorbance.

ENTER

to select

101 Absorbance 428

Absorbance

102 Absorbance 525

103 Absorbance 568

104 Absorbance 635

12:00:00 001/500

SMART3 Colorimeter 11.10 33

5. Press or to scroll to desired wavelength.

Testing

Absorbance

101 Absorbance 428

102 Absorbance 525

103 Absorbance 568

104 Absorbance 635

12:00:00 001/500

6. Press

ENTER

to select the

wavelength.

7. Insert the blank. Close the lid. Press

ENTER

to scan the blank. The screen wil display Blank Done for about 1 second and return to the Absorbance menu.

8. Insert the reacted sample. Press

ENTER

to scan the sample. The screen will display Reading for about 1 second. The result will appear on the screen.

102 Absorbance 525

Scan Blank

Scan Sample

12:00:00 001/500

102 Absorbance 525

Scan Blank

Scan Sample

12:00:00 001/500

102 Absorbance 525

0.425

Scan Blank

Scan Sample

12:00:00 001/500

34 SMART3 Colorimeter 11.10

9. To repeat the test, press to scan the sample again. The last blank scanned is used by the colorimeter for repeated scans. A different blank can be used by pressing or

to scroll to Scan Blank and then scanning another blank. Scroll with

and make another selection with Absorbance of the last test can be viewed by choosing %T/Abs. Press

escape to previous menus. NOTE: The menus loop in this screen so either or

will lead to the menu selection needed. NOTE: The calibrate function does not work in the Absorbance mode.

ENTER

EXIT

.The %T or

to

or

ENTER

102 Absorbance 525

0.425

Scan Blank

Scan Sample

12:00:00 001/500

Next Test

Previous Test

%T/Abs

Calibrate

Testing

SMART3 Colorimeter 11.10 35

EDITING MENU

The Editing Menu allows the user to edit sequences, edit user tests, set the clock, edit the logging function, access factory setting, set the power saving function, set the backlight time, and select a language.

The default factory settings are:

Date Format MM-DD-YYYY

Logging Enabled

Power Save 5 minutes

Backlight 10 seconds

Language English

 EDITING A SEQUENCE

The Edit Sequence menu allows three alterable test sequences (Sequence 1, Sequence 2, Sequence 3) to be edited.

Editing/Set Up

1. Press and brieﬂ y hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will

Testing Menu

Editing Menu

Run PC Link

Main Menu

appear.

12:00:00 001/500

2. Press or to scroll to the Editing Menu.

Testing Menu

Main Menu

Editing Menu

Run PC Link

12:00:00 001/500

3. Press Menu.

ENTER

to select Editing

Edit Sequences

Editing Menu

Edit User Test

Set Clock

Logging

12:00:00 001/500

36 SMART3 Colorimeter 11.10

4. Press Sequences.

ENTER

to select Edit

Edit Sequences

Edit Sequence 1

Edit Sequence 2

Edit Sequence 3

12:00:00 001/500

5. Press or to scroll to the desired sequence.

6. Press

ENTER

to select the

sequence to be edited.

Edit Sequences

Edit Sequence 1

Edit Sequence 2

Edit Sequence 3

12:00:00 001/500

EDIT SEQUENCE 2

015 Chlorine F UDV

079 Phosphate HR

009 Benzotriazole

076 pH UDV

12:00:00 001/500

Editing/Set Up

SMART3 Colorimeter 11.10 37

 ADDING OR DELETING A TEST

There are three ways to alter a sequence: Insert Before, Insert After, and Delete. Insert Before adds a new test to the sequence before the selected test. Insert After adds a new test to the sequence after the selected test. Delete is used to remove an existing test from a sequence.

ADDING A TEST

Below is a step-by-step example of how to add a test to SEQUENCE 2 starting from the EDIT SEQUENCE 2 menu.

1. To add a test before or after an existing test, press or to scroll to the existing test.

Editing/Set Up

2. Press

ENTER

to select the

existing test.

3. Press or to scroll to Insert Before or Insert After.

EDIT SEQUENCE 2

015 Chlorine F UDV

079 Phosphate HR

009 Benzotriazole

076 pH UDV

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

4. Press

ENTER

to select the option, Insert Before, in this example. The All Test Menu will appear.

001 Alkalinity

002 Aluminum

003 Ammonia-N LRF

All Tests

004 Ammonia-N LRS

12:00:00 001/500

38 SMART3 Colorimeter 11.10

5. Press or to scroll to the test that will be added to the sequence. In this example, Aluminum.

All Tests

001 Alkalinity UDV

002 Aluminum

003 Ammonia-N LRF

004 Ammonia-N LRS

12:00:00 001/500

6. Press

ENTER

to select the test. The sequence will appear in the Edit Sequence menu and the new test will be added to the sequence. All changes in the sequence will be automatically saved.

7. Press

EXIT

to exit the Edit Sequence menu and return

to the Editing Menu.

8. Press

ENTER

to select Edit Sequences to continue

editing the sequences or press

EXIT

to return to the

Main Menu.

EDIT SEQUENCE 2

015 Chlorine F UDV

079 Phosphate HR

002 Aluminum

009 Benzotriazole

12:00:00 001/500

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Main Menu

Testing Menu

Editing Menu

Run PC Link

Editing/Set Up

12:00:00 001/500

SMART3 Colorimeter 11.10 39

DELETING A TEST

Below is a step-by-step example of how to delete a test in SEQUENCE 2 starting from the EDIT SEQUENCE 2 menu.

1. To delete a test, press or to scroll to the test in the sequence.

2. Press

Editing/Set Up

ENTER

to select the test.

3. Press or to scroll to Delete.

EDIT SEQUENCE 2

015 Chlorine F UDV

079 Phosphate HR

002 Aluminum

009 Benzotriazole

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

4. Press

ENTER

to select Delete. The sequence will appear in the EDIT SEQUENCE menu and the selected test will have been deleted. All

015 Chlorine F UDV

079 Phosphate HR

002 Aluminum

EDIT SEQUENCE 2

changes to the sequence will automatically have been saved.

40 SMART3 Colorimeter 11.10

12:00:00 001/500

5. Press

EXIT

to exit the Edit

Sequence menu and return

to the Editing Menu.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

6. Press

ENTER

to select Edit

Sequences to continue

editing the sequences or press

EXIT

to return to the

Main Menu.

Main Menu

Testing Menu

Editing Menu

Run PC Link

12:00:00 001/500

Editing/Set Up

SMART3 Colorimeter 11.10 41

 EDIT USER TESTS

If a test other than the LaMotte programmed tests is performed regularly, a calibration for it may be entered in one of the 25 User Tests. These tests are originally named “User Test 1 - 25”. It will be possible to rename the test, select a wavelength, enter a new calibration, select the number of decimal places used to display the results, and select the units. A User Test may be added for a reagent system for which no precalibrated test exists. A calibration of a LaMotte reagent system may also be entered. The calibration of a User Test can be changed at any time.

The User Tests have the ability to handle 2 data points. The colorimeter will determine the absorbance of the standards and calculate a response that will be stored to determine the concentration of future samples of unknown concentration. These standards should cover all the concentrations for the range of the test being performed and be scanned beginning with the low concentration and ﬁ nishing with the high concentration (for more information about this, see CALIBRATION CURVES, page 12). Prepare these standards prior to entering a new calibration.

NOTE: A calibration procedure must be performed before using any of the User

Editing/Set Up

Tests.

The User Tests can be placed in any of the alterable sequences using Edit Sequences.

1. Press and brieﬂ y hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will

Testing Menu

Editing Menu

Run PC Link

Main Menu

appear.

12:00:00 001/500

2. Press or to scroll to the Editing Menu.

Testing Menu

Main Menu

Editing Menu

Run PC Link

12:00:00 001/500

42 SMART3 Colorimeter 11.10

3. Press

ENTER

to select Editing Menu. Press to scroll to Edit User Test.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

4. Press

ENTER

to select Edit User Test.

5. Press or to scroll to the desired user test.

6. Press

ENTER

to select the User

Test.

Edit User Test

105 USER TEST 01

106 USER TEST 02

107 USER TEST 03

108 USER TEST 04

12:00:00 001/500

Edit User Test

108 USER TEST 04

109 USER TEST 05

110 USER TEST 06

111 USER TEST 07

12:00:00 001/500

111 USER TEST 07

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

Editing/Set Up

12:00:00 001/500

SMART3 Colorimeter 11.10 43

 NAMING THE TEST

A User Test can be up to 16 characters long. The menu choices for each character are 26 upper case letters A to Z, 26 lower case letters a to z, ten numerals 0 to 9, a space, a dash (-) and a decimal point (.). The existing name is displayed on the bottom line of the display. The character which is to be edited will blink and that character is also displayed in the center of the display. The

character can be changed by using or to scroll to other characters.

ENTER

Use pressing

to select a character. The edited name is saved at any time by

EXIT

or by pressing

ENTER

after selecting the sixteenth character.

1. From the User Test menu, press to scroll to Name the Test.

Editing/Set Up

2. Press

ENTER

to select Name

the Test. A reverse font

(dark background with a light character) will appear to indicate the character that will be adjusted. The same character will also appear in the center of the display.

3. Press or to scroll to the desired character. In this example, the test name will be H2O.

111 USER TEST 07

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

Name the Test

R S T U V W X

111 U SER TEST 07

12:00:00 001/500

Name the Test

E F G H I J K

111 U SER TEST 07

12:00:00 001/500

44 SMART3 Colorimeter 11.10

4. Press

ENTER

to save the character and move to the next character.

Name the Test

P Q R S T U V

111 H S ER TEST 07

12:00:00 001/500

5. Press or to scroll to the desired character.

6. Press

ENTER

to save the character and move to the next character.

7. Press or to scroll to the desired character.

Name the Test

Editing/Set Up

. 0 1 2 3 4 5

111 H S ER TEST 07

12:00:00 001/500

Name the Test

L M N E P Q R

111 H2 E R TEST 07

12:00:00 001/500

Name the Test

L M N O P Q R

111 H2 O R TEST 07

12:00:00 001/500

SMART3 Colorimeter 11.10 45

8. Press

ENTER

to save the character. Repeat the procedure until the test name is complete. To remove a character, change the character to a space (located after the letter z). Press

to save the name. The sreen will display Storing... and the test name for about 1 second and the meter will return to the Edit Test menu.

Editing/Set Up

EXIT

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

46 SMART3 Colorimeter 11.10

 SELECTING THE VIAL AND WAVELENGTH

The SMART3 Colorimeter accepts three different vials (the 25 mm 0290 tube, UDVs and COD tubes) at 4 different wavelengths (428, 525, 560, and 635 nm). The colorimeter uses different settings for each of the twelve combinations of vial and wavelength. These twelve settings are called channels. Choose the channel with the correct wavelength and vial for the test.

1. From the User Test menu, press or to scroll to Select Vial/WL.

2. Press

ENTER

to select Select

Vial/WL.

3. Press or to select the channel with the desired wavelingth and vial size combination.

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

Select Channel

Ch1 428nm 25mm

Ch2 525nm 25mm

Ch3 635nm 25mm

Ch4 568nm 25mm

12:00:00 001/500

Select Channel

Ch1 428nm 25mm

Ch2 525nm 25mm

Ch3 635nm 25mm

Ch4 568nm 25mm

12:00:00 001/500

Editing/Set Up

4. Press

ENTER

to select the channel. The screen will display Storing... for about 1 second and the meter will return to the Edit Test menu.

Name the Test

Select Vial/WL

STD Calibration

111 H2O

Enter Constants

12:00:00 001/500

SMART3 Colorimeter 11.10 47

 ENTERING A TWO POINT CALIBRATION

The SMART3 Colorimeter can scan two reacted standards and create a calibration curve. To prepare a calibration curve with multiple data points see Entering a Multiple Calibration Curve (pg. 51).

1. From the User Test menu, press or to scroll to STD (Standard) Calibration.

2. Press

ENTER

to select STD

Calibration. The screen will

display Low Standard for about 1 second and then

Editing/Set Up

display the Low Standard screen. A reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will also appear in the center of the display.

3. Press or to scroll to the ﬁ rst character of the low concentration. In this example, 1.00 ppm.

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

Low Standard

9 . – 0 1 2 3

0 0.000000

12:00:00 001/500

Low Standard

. – 0 1 1 2 3

0 .000000

12:00:00 001/500

48 SMART3 Colorimeter 11.10

4. Press

ENTER

to save the character and move to the next character.

Low Standard

7 8 9 . – 0 1

1 . 000000

12:00:00 001/500

5. Press or to scroll to the desired character.

6. Press

ENTER

to save the character and move to the next character.

7. Press or to scroll to the desired character.

Low Standard

Editing/Set Up

7 8 9 . – 0 1

1 . 000000

12:00:00 001/500

Low Standard

9 . – 0 1 2 3

1. 0 00000

12:00:00 001/500

Low Standard

9 . – 0 1 2 3

1. 0 00000

12:00:00 001/500

SMART3 Colorimeter 11.10 49

8. Press

ENTER

to save the character. Repeat the procedure until the low concentration value is complete. After the ﬁ nal character is complete the meter will save the low concentration value. The screen will display High Standard for about 1 minute and the meter will display the High Standard screen. A reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will appear in the center of the

Editing/Set Up

display.

High Standard

9 . – 0 1 2 3

.0000

0

12:00:00 001/500

9. Use or and to select the characters for the high concentration value. In this example, 7.5 ppm.

10. After the ﬁ nal character is entered the meter will save the high concentration value. The screen will display instructions for completing the calibration procedure.

ENTER

High Standard

9 . – 0 1 2 3

7.50000 0

12:00:00 001/500

Insert Blank

<Enter> continue

12:00:00 001/500

50 SMART3 Colorimeter 11.10

11. Insert the blank. Press

ENTER

The screen will display Blank Done... for about 1 second

and the Insert Low Standard screen will appear.

12. Insert the low standard. Press

ENTER

. The screen will display Reading... for about 1 second and the Insert High Standard screen will be displayed.

.

Insert Low Standard

<Enter> continue

12:00:00 001/500

Insert High Standard

<Enter> continue

12:00:00 001/500

Editing/Set Up

13. Insert the high standard.

ENTER

Press

. The screen will display Reading... for about 1 second and the meter will return to the Edit Test menu.

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

SMART3 Colorimeter 11.10 51

 ENTERING A MULTIPLE POINT CALIBRATION

The SMART3 can directly create a 2 point calibration curve. (See Entering a Two Point Calibration on page 47.) To create a multiple point calibration curve, constants obtained from a linear regression of multiple data points can be entered into the SMART 3.

1. Scan reactions of multiple concentrations at the appropriate wavelength in the absorbance mode on the SMART3.

2. Plot the concentration (y axis) versus absorbance (x axis) in a program capable of linear regression such as Excel.

3. Enter the constants obtained from the linear regression equation into the SMART3.

For Example:

3

y = 0.001x

K0 = – 0.049

K1 = 0.181

Editing/Set Up

K2 = – 0.017

K3 = 0.001

OR (Over Range) = 10

– 0.017x2 + 0.181x – 0.049

1. From the User Test menu, press or to scroll to Enter Constants.

Name the Test

Select Vial/WL

111 H2O

STD Calibration

Enter Constants

12:00:00 001/500

2. Press Constants.

ENTER

to select Enter

K0=0.00000

K1=0.00000

K2=0.00000

K3=0.00000

OR=100.00000

12:00:00 001/500

52 SMART3 Colorimeter 11.10

3. Press

ENTER

to begin entering the values for the constants. A reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will also appear in the center of the display.

Enter K0

9 . – 0 1 2 3

.00000

0

12:00:00 001/500

4. Press or to scroll to the ﬁ rst character of K0. In this example, – 0.049.

5. Press

ENTER

to save the character and move to the next character.

6. Press or to scroll to the next character.

Enter K0

Editing/Set Up

8 9 . – 1 2 3

0 .00000

12:00:00 001/500

Enter K0

7 8 9 . – 0 1

– . 00000

12:00:00 001/500

Enter K0

9 . – O 1 2 3

– . 00000

12:00:00 001/500

7. Press

ENTER

to save the

Enter K0

character and move to the next character. Press or

to scroll to the next

character.

SMART3 Colorimeter 11.10 53

7 8 9 . – 0 1

– 0 0 0000

12:00:00 001/500

8. Press

ENTER

to save the character. Repeat the procedure until the K0 value is complete. After the ﬁ nal character is complete the meter will save the K0 value and the meter will display K1 screen.

Enter K1

9 . – 0 1 2 3

.00000

0

12:00:00 001/500

9. Use , and to select the characters for the remaining values: K1, K2, K3, and over range. In this example, 10 ppm.

Editing/Set Up

10. After the ﬁ nal character is entered the meter will save the constants. The screen will display Storing... and return to the Edit Test menu.

ENTER

Enter Overrange

9 . – 0 1 2 3

10.00000 0

12:00:00 001/500

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

54 SMART3 Colorimeter 11.10

 SELECTING THE NUMERICAL FORMAT OF THE

RESULT

To input tests with very different ranges, the number of decimal places displayed for a result can be selected. A test which ranges from 20 to 1000 ppm should not be displayed with three decimal places. A test with a range from 0.010 to

0.500 needs three decimal places (the microprocessor will always calculate the concentration to many more signiﬁ cant ﬁ gures than will be displayed). The choice of 0, 1, 2, or 3 decimal places are available.

1. From the User Test menu, press or to scroll to Decimal Places.

2. Press

ENTER

to select Decimal

Places.

3. Press or to scroll to the desired number of decimal places.

111 H2O

Select Vial/WL

STD Calibration

Enter Constants

Decimal Places

12:00:00 001/500

Decimal Places?

None 0

One 0.0

Two 0.00

Three 0.000

12:00:00 001/500

Decimal Places?

None 0

One 0.0

Two 0.00

Three 0.000

12:00:00 001/500

Editing/Set Up

4. Press

ENTER

to select the decimal places. The screen wil display Storing... for about 1 second and the meter will return to the Edit Test menu.

Select Vial/WL

STD Calibration

Enter Constants

111 H2O

Decimal Places

12:00:00 001/500

SMART3 Colorimeter 11.10 55

 SELECTING THE UNITS OF CONCENTRATION

The SMART3 Colorimeter has seven options for units of concentration. They are No Units, ppm, FAU, pH, ppb, ppt and mgL.

1. From the User Menu, press to scroll to Select

Units.

2. Press

ENTER

to select Select

Units.

Editing/Set Up

3. Press or to scroll

to the desired units.

111 H2O

STD Calibration

Enter Constants

Decimal Places

Select Units

12:00:00 001/500

Select Units

No Units

ppm

pH

FAU

12:00:00 001/500

Select Units

No Units

ppm

pH

FAU

12:00:00 001/500

4. Press

ENTER

to select the units. The screen will display Storing... for about 1 second and the meter will return to the Edit Test menu.

STD Calibration

Enter Constants

Decimal Places

111 H2O

Select Units

12:00:00 001/500

56 SMART3 Colorimeter 11.10

 SETTING THE CLOCK

Setting the clock allows the correct time and date stamp to be stored with each reading in the data logger.

1. From the Editing Menu, press or to scroll to Set

Clock.

2. Press

ENTER

to select Set Clock. The date is displayed as month-dayyear. The time is displayed as hours:minutes:seconds AM/PM. Press or to scroll to the appropriate character. Press

ENTER

to select the character. The curser will move to the next character. Set all characters in the same manner. The character menu is a scrolling menu.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Editing/Set Up

Set Time

Date: MM-DD-YYYY

Time: HH-MM-SS AM/PM

12:00:00 001/500

3. Press

ENTER

to select the ﬁ nal character. The time and date will be saved and the meter will return to the Edit Test menu.

Edit Sequences

Edit User Test

Set Clock

Editing Menu

Logging

12:00:00 001/500

SMART3 Colorimeter 11.10 57

 LOGGING DATA

The default setting for the data logger is enabled. The meter will log the last 500 data points. The counter in the center bottom of the display will show how many data points have been logged. The display will show 500+ when the data logger has exceeded 500 points and the data points are being overwritten.

1. From the Editing Menu, press or to scroll to

Logging.

2. Press

ENTER

to select

Logging.

Editing/Set Up

3. Press or to scroll

to desired function.

Editing Menu

Edit Sequence

Edit User Test

Set Clock

Logging

12:00:00 001/500

Logging

Display Test Log

Enable Logging

Disable Logging

Erase Log

12:00:00 001/500

Logging

Display Test Log

Enable Logging

Disable Logging

Erase Log

12:00:00 001/500

4. Press

ENTER

. The screen will display Storing... for about 1 second and return to the Logging menu.

Display Test Log

Enable Logging

Disable Logging

Logging

Erase Log

12:00:00 001/500

58 SMART3 Colorimeter 11.10

5. Press Editing Menu.

EXIT

to return to the

Edit Sequence

Editing Menu

Edit User Test

Set Clock

Logging

12:00:00 001/500

 FACTORY SETUP

The Factory Setup menu is used in manufacturing of the SMART3 Colorimeter. This menu is not for use by the operator in the ﬁ eld.

 SETTING POWER SAVE

The power saving Auto Shutoff feature will turn the meter off when a button has not been pushed for a set amount of time. The default setting is disabled. To change the setting:

Editing/Set Up

1. From the Editing Menu, press

or to scroll to Set

PWR Save.

2. Press

ENTER

to select Set

PWR Save.

3. Press or to scroll to desired function.

Editing Menu

Set Clock

Logging

Factory Setup

Set PWR Save

12:00:00 001/500

Auto Shutoff

Disable

5 Minutes

15 Minutes

30 Minutes

12:00:00 001/500

Auto Shutoff

Disable

5 Minutes

15 Minutes

30 Minutes

12:00:00 001/500

SMART3 Colorimeter 11.10 59

4. Press

ENTER

. The screen will display Storing... for about 1 second and the meter will return to the Editing Menu.

Editing/Set Up

Editing Menu

Set Clock

Logging

Factory Setup

Set PWR Save

12:00:00 001/500

60 SMART3 Colorimeter 11.10

 SETTING THE BACKLIGHT TIME

The backlight illuminates the display for enhanced viewing. The default setting is 10 seconds. If Button Control is chosen the backlight button on the key pad will act as an on/off switch and the backlight will remain on or off when the meter is being used. When one of the other settings – 10, 20 or 30 seconds – is chosen, the display will be illuminated for the speciﬁ ed amount of time after any button is pressed. NOTE: The backlight feature uses a signiﬁ cant amount of power. The longer the backlight is on, the more frequently the battery will have to be charged if the USB/Wall Adapter is not being used.

1. From the Editing Menu, press or to scroll to

Backlight Time.

2. Press

ENTER

to select Set

Backlight Time.

3. Press or to scroll

to desired option.

Editing Menu

Logging

Factory Setup

Set PWR Save

Set Backlight Time

12:00:00 001/500

Backlight Time

Button Control

10 seconds

20 seconds

30 seconds

12:00:00 001/500

Backlight Time

Button Control

10 seconds

20 seconds

30 seconds

12:00:00 001/500

Editing/Set Up

4. Press

ENTER

. The screen will display Storing... for about 1 second and the meter will return to the Editing Menu.

Logging

Factory Setup

Set PWR Save

Editing Menu

Set Backlight Time

12:00:00 001/500

SMART3 Colorimeter 11.10 61

 SELECTING A LANGUAGE

There are seven languages available in the SMART3: English, Spanish, French, Portuguese, Italian, Chinese, and Japanese.

1. From the Editing Menu, press or to scroll to

Select Language.

2. Press

ENTER

to select Select

Language.

Editing/Set Up

3. Press or to scroll

to desired language.

Editing Menu

Factory Setup

Set PWR Save

Set Backlight Time

Select Language

12:00:00 001/500

Select Language

English

Spanish

French

Portugese

12:00:00 001/500

Select Language

English

Spanish

French

Portugese

12:00:00 001/500

4. Press

ENTER

. The screen will display Storing... for about 1 second and the meter will return to the Editing Menu.

Factory Setup

Set PWR Save

Set Backlight Time

Editing Menu

Select Language

12:00:00 001/500

62 SMART3 Colorimeter 11.10

NOTE: If meter unintentionally switches to another language, use the procedure above to reset the meter to the desired language. For example, to reset the meter to English:

1. Turn meter on.

2. Press one time. Press

3. Press

4. Press

seven times. Press

ENTER

.

ENTER

.

ENTER

.

Editing/Set Up

SMART3 Colorimeter 11.10 63

COMPUTER CONNECTION

 PC LINK

The SMART3 may be interfaced with any Windows-based computer by using the LaMotte SMARTLink 3 Program and USB Cable. The program will store test information and results in a database. To transfer data from the meter to a computer, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB port on a computer. The SMART3 will send the following data: test name, wavelength, concentration, transmittance, absorbance, sample, blank, time of test, and date of test.

 OUTPUT

USB

 COMPUTER CONNECTION

USB Type A, USB mini B, Order Cable Code 1720.

 SMARTLINK3

SmartLink3 records the above data and appends a test ID# which uniquely identiﬁ es the test in the database, the serial number of the meter, and a site ID# which can be used to associate the test record with a site or customer via the SmartLink3 program. It also stores a “test number” which is useful for the SMART3.

64 SMART3 Colorimeter 11.10

BATTERY

 BATTERY/AC OPERATION

The SMART3 may be operated on battery power, using a USB wall adapter or USB computer connection. If using the meter as a bench top unit, use the wall adapter if possible to extend the battery life. The meter will remain on when the USB adapter is used.

To charge the battery with the wall adapter, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into the wall adapter. Plug the wall adapter into an AC outlet. Reinsert the USB port plug after charging.

To charge the battery from a computer, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB port on a computer.

The battery icon will show no bars and ﬂ ash when the unit ﬁ rst turns on. Then the indicator will indicate the battery status by showing 0, 1, 2, 3 or 4 bars.

It will take 5 hours to fully charge a low battery. The battery icon will ﬂ ash when the battery is charging. The battery icon will show four bars and stop ﬂ ashing when it is fully charged. The charging circuit will automatically switch to a ﬂ oat charge when the battery is fully charged. The charger may remain connected. Some computers will NOT supply power to their USB ports during standby operation. The wall adapter will charge the unit continuously.

The battery icon will show no bars and continuously ﬂ ash if the battery is getting low but the unit will still operate normally. A “Low Battery” message on the status bar of the display will replace the time when the battery voltage is too low for proper operation and accuracy may be degraded. A “Shutdown Low Batt” message on the display will appear for a few seconds before the power is switched off when the battery is too low to operate the unit.

To extend the battery life:

• Shut down the unit with the power switch when not taking measurements or use the power save option to have the unit automatically turn off after 5 minutes.

• Store the unit in a cool dry place.

• Fully charge the battery before storing the unit for extended periods of time.

• Limit backlight use. The unit consumes 3X normal power with the backlight on. Set the backlight time option to 10 seconds, or select “Button Control” and keep the backlight off.

Battery replacement: The lithium ion battery used in this unit should last for many years with normal use. When it no longer powers the unit long enough to meet testing requirements it will need to be replaced. Lithium ion batteries that are properly charged and stored do not usually lose all capacity; they just have

SMART3 Colorimeter 11.10 65

less capacity after hundreds of charge cycles. This unit uses a custom battery assembly that is only available from LaMotte Company. Battery replacement must be performed at a LaMotte authorized repair facility. The water resistant housing of this meter should not be opened by the user. Contact LaMotte Company by phone (1-800-344-3100) or email (tech@lamotte.com) for a return authorization number.

MAINTENANCE

 CLEANING

Clean the exterior housing with a damp, lint-free cloth. Do not allow water to enter the light chamber or any other parts of the meter. To clean the light chamber and optics area, point a can of compressed air into the light chamber and blow the pressurized air into the light chamber. Use a cotton swab dampened with Windex® window cleaner to gently swab the interior of the chamber. Do not use alcohol; it will leave a thin residue over the optics when dry.

 REPAIRS

Should it be necessary to return the meter for repair or servicing, pack the meter carefully in a suitable container with adequate packing material. A return authorization number must be obtained from LaMotte Company by calling 800344-3100 (US only) or 410-778-3100, faxing 410-778-6394, or emailing tech@ lamotte.com. Often a problem can be resolved over the phone or by email. If a return of the meter is necessary, attach a letter with the return authorization number, meter serial number, a brief description of problem and contact information including phone and FAX numbers to the shipping carton. This information will enable the service department to make the required repairs more efﬁ ciently.

 METER DISPOSAL

Waste Electrical and Electronic Equipment (WEEE)

Natural resources were used in the production of this equipment. This equipment may contain materials that are hazardous to health and the environment. To avoid harm to the environment and natural resources, the use of appropriate take-back systems is recommended. The crossed out wheeled bin symbol on the meter encourages the use of these systems when disposing of this equipment.

Take-back systems will allow the materials to be reused or recycled in a way that will not harm the environment. For more information on approved collection, reuse, and recycling systems contact local or regional waste administration or recycling services.

66 SMART3 Colorimeter 11.10

TROUBLESHOOTING

 ERROR MESSAGES

OVER RANGE

If the message OVERRANGE is displayed when scanning a sample, the sample may be over range or under range. If the sample is over range the sample should be diluted and tested again (see Sample Dilution Techniques and Volumetric Measurements, page 16).

If overrange is displayed, press

ENTER

to continue testing on

diluted samples.

Note: After pressing

ENTER

, the overrange cncentration will be displayed. This concentration is an approximation only.

Overrange

<Enter> continue

Scan Blank

Scan Sample

12:00:00 001/500

002 Aluminum

CALIBRATION

As with all pre-calibrated meters, it is highly recommended, even if not required by regulations, that the user periodically verify the performance of the meter by running standards with a predetermined concentration. Results outside of speciﬁ cation are an indication that the meter needs to be adjusted. This can be done following the user calibration described on page 28. If the user calibration fails to properly adjust the meter then the meter should be returned to LaMotte Company for recalibration. (See page 65).

STRAY LIGHT

The SMART3 Colorimeter should have no problems with stray light. Make sure that the sample compartment lid is always fully closed, except when testing COD with the adapter.

SMART3 Colorimeter 11.10 67

 TROUBLESHOOTING GUIDE

PROBLEM REASON SOLUTION

Flashing

Low battery. Readings are reliable.

“Low Battery” Battery voltage is very

low. Readings are not reliable.

“Shut Down Low Batt” Shut Down

Battery is too low to operate the unit.

“Overrange” Sample is outside of

acceptable range.

Charge battery or use USB wall/computer adapter.

Dilute sample and test again.

Unusually large negative or positive readings when performing calibration

Incorrect standards used to calibrate meter.

Use fresh 0.0 standard in clean tube. Reset meter to factory default settings. Recalibrate meter.

68 SMART3 Colorimeter 11.10

1910-TEST

02.13.12

SMART3 COLORIMETER REAGENT SYSTEMS

SMART3 REAGENT SYSTEMS LIST

LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available. Pre-programmed calibrations can be added to the SMART3 Colorimeter in the ﬁ eld. A Windows-based computer running a Windows Operating System and an 8 pin mini-DIN/9 pin F D-submin serial cable (order Code

1771) are required.

Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or email at tech@lamotte.com for a current list of available calibrations and downloading instructions.

Range

Test Factor (Test #)

Alkalinity-UDV (001) 0–200 10 Unit Dose Vials (1) 50

Aluminum (002) 0.00–0.30 0.01 Eriochrome Cyanine R (4) 50

Ammonia NitrogenLow Range, Fresh Water (003)

Ammonia NitrogenLow Range, Salt Water (004)

Ammonia NitrogenHigh Range (005)

Barium 0-200 5 Barium Chloride (1) 50

Benzotriazole (009) 0.0–30.0 0.5 UV Photolysis (3) 50

Biguanide (006) 0–70 2 Colorimetric (1) 50

Borate-UDV (007) 0.0–80.0 5.0 Unit Dose Vial (1) 50

Boron (008) 0.00-0.80 0.05 Azomethine-H (2) 50

Bromine-Low Range (010) 0.00–9.00 0.1 DPD (3) 100

Bromine-UDV (011) 0.0–20.0 0.25 DPD (1) 50

Cadmium (012) 0.00–1.00 0.04 PAN (4) 50

Carbohydrazide (013)

See Oxygen Scavengers

Chloride-TesTab (020) 0.0–30.0 0.4 Argentometric (1) 50

Chlorine-Liquid DPD (017) 0.00–4.00 0.03 DPD (3) 144

Chlorine-Tablet DPD (014) 0.00–4.00 0.03 DPD (3) 100

Chlorine-Free-UDV (015) 0.00–10.00 0.10 DPD (1) 50

Chlorine-Total-UDV (18) 0.00–10.00 0.10 DPD (1) 50

Chlorine Dioxide (019) 0.00–8.00 0.10 DPD (2) 100

Chromium, Hexavalent (021) 0.00–1.00 0.01 Diphenylcarbohydrazide (1) 50

Chromium, Hex, Tri, Total (021) 0.00–1.00 0.01 Diphenylcarbohydrazide (5) 50

(ppm) MDL Test Method (# of Reagents)

0.00–1.00 0.05 Salicylate (3) 25

0.00–1.00 0.10 Salicylate (3) 25

0.00–4.00 0.05 Nesslerization (2) 50

0.000–0.900 0.01 Iron Reduction (3) 100

# of Tests

Cobalt (023) 0.00–2.00 0.04 PAN (3) 50

COD-Low Range (024) 0–150 7.5 Digestion (1) 25

COD-Standard Range (025) 0-1500 40 Digestion (1) 25

COD-High Range (026) 0–15000 400 Digestion (1) 25

Color (027) 0–1000 20 Platinum Cobalt (0) –

Copper-BCA-Low Range (028) 0.00–3.50 0.04 Bicinchoninic Acid (1) 50

Copper-Cuprizone (030) 0.00–2.50 0.03 Cuprizone (2) 50

Copper-DDC (031) 0.00–7.00 0.10 Diethyldithiocarbamate (1) 50

Copper-UDV (032) 0.0–4.0 0.1 Bicinchoninic Acid (1) 50

Cyanide (034) 0.00-0.50 0.01 Pyridine-Barbituric Acid (5) 50

Cyanuric Acid (035) 5–200 10 Melamine (1) 50

Cyanuric Acid-UDV (036) 5–150 10 Melamine (1) 50

DEHA (037) See Oxygen Scavengers

Dissolved Oxygen (038) 0.0–10.0 0.6 Winkler Colorimetric (3) 100

Erythorbic Acid (049) See Oxygen Scavengers

Fluoride (040) 0.00–2.00 0.10 SPADNS (2) 50

Hardness (Total) UDV (043) 0–450 10 Unit dose Vial (1) 50

Hydrazine (045) 0.00–1.00 0.01 P-dimethyl-

Hydrogen PeroxideLow Range (046)

Hydrogen PeroxideHigh Range (047)

Hydrogen Peroxide-Shock (048) 0–300 5 DPD (2) 100

Hydroquinone (049) See Oxygen Scavengers

Iodine (050) 0.00–14.00 0.15 DPD (2) 100

Iron-Bipyridyl (051) 0.00–6.00 0.10 Bipyridyl (2) 50

Iron-Total, Ferrous, Ferric (053) 0.00–5.00 0.06 1,10 Phenanthroline (2) 50

Iron-UDV (052) 0.00–10.00 0.05 Bipyridyl (1) 50

Lead (054) 0.00–5.00 0.10 PAR (5) 50

Manganese-Low Range (055) 0.00–0.70 0.01 PAN (3) 50

Manganese-High Range (056) 0.0–15.0 – Periodate (2) 50

Mercury (057) 0.00–1.50 0.01 TMK (3) 50

Methylethylketoxime (058) See Oxygen Scavengers

Molybdenum-High Range (061) 0.0–50.0 0.6 Thioglycolate (3) 50

Nickel (063) 0.00–8.00 0.15 Dimethylglyoxime (6) 50

0.000–0.700 0.01 Iron Reduction (3) 100

0.00–3.00 0.02 Iron Reduction (3) 100

50

aminobenzaldehyde (2)

0.00–1.50 0.02 DPD (2) 100

0.0–80.0 0.5 DPD (2) 50

0.00–2.00 0.01 Iron Reduction (3) 100

0.00–3.00 0.01 Iron Reduction (3) 100

Nitrate Nitrogen-Low Range (064) 0.00–3.00 0.10 Cadmium Reduction (2) 20

Nitrate TesTab (065) 0-60 5 Zinc Reduction (1) 50

Nitrate-UDV (066) 0.00-80.0 2 Zinc Reduction (1) 100

Nitrite Nitrogen-Low Range (067) 0.00–0.80 0.02 Diazotization (2) 20

Nitrogen, Total (069) 3-25 mg/L 3 mg/L Chromotropic Acid/Digestion

(6)

Oxygen Scanvengers various various DEHA (3) 50

Ozone-DPD (070) 0.00–3.00 0.03 DPD (3) 144

Ozone-Low Range (071) 0.00–0.40 0.02 Indigo Trisulfonate (3) 100

Ozone-High Range (072) 0.00–3.00 0.05 Indigo Trisulfonate (3) 20

pH-Chlorophenol Red (073) 5.0–6.8 – Chlorophenol Red (1) 100

pH-Phenol Red (074) 6.6–8.4 – Phenol Red (1) 100

pH-Thymol Blue (075) 8.0–9.6 – Thymol Blue (1) 100

Phenol (077) 0.00-6.00 0.05 Aminoabtipyrine (2) 50

Phosphate-Low Range (078) 0.00–3.00 0.05 Ascorbic Acid Reduction (2) 25

Phosphate-High Range (079) 0.0–70.0 0.5 Vanodomolybd-

phosphoric Acid (1)

Phosphorus, ppb (080) 0–3000 ppb 50 Ascorbic Acid/Reduction (5) 50

Phosphorus, Total, Low Range (081)

Phosphorus, Total, High-Range (082)

Potassium (083) 0.0-10.0 0.8 Tetraphenylboron (2) 100

Silica-Low Range (085) 0.0–4.0 0.05 Heteropoly Blue (4) 50

Silica-High Range (086) 0–75 0.5 Silicomolybdate (3) 50

Sulfate-High Range (089) 0–100 3 Barium Chloride (1) 50

Sulﬁ de-Low Range (090) 0.00–1.50 0.06 Methylene Blue (3) 50

Surfactants (094) 0.00-8.00 0.75 Bromphenol Blue (3) 100

Tannin (096) 0.0–10.0 0.1 Tungsto-molybdophosphoric

Tolytriazole (009) See Benzotriazole

Turbidity (098) 0.0–30.0 FTU 3 Absorption (0) –

Zinc-Low Range (099) 0.00–3.00 0.05 Zincon (6) 50

0.00–3.50 mg/L

0.0–70.0 mg/L

0.0–30.0 0.5 UV Photolysis (3) 50

0.50 Ascorbic Acid/Digestion 25

5 Molybdovanadate/Digestion

(5)

Acid (2)

25

50

ALKALINITY–UDV

UNIT DOSE VIALS • CODE 4318-J

QUANTITY CONTENTS CODE

1 Alkalinity Unit Dose Vials, 20 pouches 4318-J

Equipment needed but not supplied:

STANDARD ACCESSORY PACKAGE · CODE 1961

1 Package of 3 Vials (empty) 0156

1 Syringe, 3 mL, plastic 1184

1 Foil Storage Bag 9467

Or:

ADVANCED ACCESSORY PACKAGE · CODE 1962

1 Pipettor, 3mL 30528

1 Pipet Tip (0-5 mL) 30695

1 Cuvette Rack 31695

1 Package of 3 Vials (empty) 0156

1 Foil Storage Bag 9467

Test Procedures

APPLICATION: Drinking and surface waters; swimming pool water.

RANGE: 0–200 ppm as CaCO

3

MDL: 10 ppm

METHOD The sample is added to a buffered indicator reagent.

The color that develops, ranging from yellow to blue, will indicate the amount of alkalinity in the sample.

SAMPLE HANDLING & PRESERVATION:

Samples should be analyzed as soon as possible after collection. Sample may be refrigerated for 24 hours.

INTERFERENCES: Quats and poly quats at high concentrations will interfere.

SMART3 Test Procedures 11.10 ALKALINITY, UDV

PROCEDURE

Use COD/UDV adapter

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 001 Alkalinity UDV) from

4. Scroll to and select 001 Alkalinity UDV from menu.

5. Rinse a clean vial (0156) with sample water.

6. Use the syringe (1184) to add 3 mL of sample to the vial.

7. Insert the vial into chamber, close lid and select SCAN BLANK.

8. Remove vial from the colorimeter.

9. Use the syringe (1184) to add 3 mL of sample to an Alkalinity-UDV vial (4318).

10. Wait 90 seconds.

11. Invert vial 3 times to mix.

12. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result.

13. Press

NOTES: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized

Test Procedures

water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

ENTER

to select TESTING MENU.

TESTING MENU.

NOTE: If powder residue remains in the bottom of the vial after inverting, invert once more and tap bottom of vial sharply once or twice to dislodge powder. Mix.

to turn the colorimeter off or press

EXIT

to exit to a previous

menu or make another menu selection.

UDVs from opened pouches should be used promptly. Store unused vials from opened pouches in the Foil Storage Bag (9467) to extend the shelf life of the reagent. Generally, UDVs stored in the bag should be used within 10 days if the humidity is less than 50% and within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced.

ALKALINITY, UDV SMART3 Test Procedures 11.10

ALUMINUM

ERIOCHROME CYANINE R METHOD CODE 364I-01-SC

QUANTITY CONTENTS CODE

5 g *Aluminum Inhibitor Reagent *7865-C

2 x 120 mL *Aluminum Buffer Reagent *7866-J

120 mL Aluminum Indicator Reagent 7867-J

15 mL Aluminum Complexing Reagent 7868-E

1 Spoon, 0.05 g, plastic 0696

2 Pipets, 1.0 mL, plastic 0354

1 Test Tube, glass, 5 mL w/cap 0230

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Aluminum is the third most common element in the earth’s crust, which accounts for its wide appearance in many water supplies. Aluminum exists in water as soluble salts, colloidal compounds, and insoluble compounds. In wastewater that has been treated by alum coagulation it will appear in one or more of the above forms. Properly treated drinking water should have an aluminum concentration below 0.05 mg/L.

Test Procedures

APPLICATION: Drinking, surface, and saline waters; domestic and

industrial wastewater.

RANGE: 0.00–0.30 ppm Aluminum

MDL: 0.01 ppm

METHOD: Aluminum ions buffered to a pH of 6.0 react with

Eriochrome Cyanine R dye to produce a pink to red complex in proportion to the concentration.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Fluoride and polyphosphate will interfere. Interference

SMART3 Test Procedures 11.10 ALUMINUM

Collect sample in acid washed glass or plastic bottle. Analyze as soon as possible.

from iron and manganese is eliminated by the addition of an inhibitor.

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 002 Aluminum).

4. Scroll to and select 002 Aluminum from menu.

5. Rinse a clean colorimeter tube (0290) with sample water. Fill to the 10 mL line

6. Insert tube into colorimeter chamber and select SCAN BLANK.

7. Rinse a clean test tube (0230) with sample water. Fill to the 5 mL line with

8. Remove tube from colorimeter. Empty sample from tube (0290).

9. Add 5 mL sample from test tube (0230) to empty tube (0290).

10. Use the 0.05 g spoon (0696) to add one measure of *Aluminum Inhibitor

11. Use a 1.0 mL pipet (0354) to add 2 mL of *Aluminum Buffer Reagent (7866).

12. Use a second 1.0 mL pipet (0354) to add 1 mL of Aluminum Indicator Reagent

13. At end of 5 minute waiting period, mix, insert tube into chamber, close lid and

14. Press

NOTE: For the best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized

Test Procedures

water blank. Add 5 drops of Aluminum Complexing Reagent (7868). Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

ENTER

to select TESTING MENU.

with sample.

sample.

Reagent (7865). Cap and mix to dissolve powder.

Cap and mix.

(7867). Cap and mix contents. Wait 5 minutes for maximum color development.

select SCAN SAMPLE. Record result.

to turn the colorimeter off or press

EXIT

to exit to a previous

menu or make another menu selection.

ALUMINUM SMART3 Test Procedures 11.10

AMMONIA NITROGEN - LOW RANGE

SALICYLATE METHOD • CODE 3659-01-SC

QUANTITY CONTENTS CODE

60 mL *Salicylate Ammonia #1 *3978-H

10 g *Salicylate #2 *7457-D

2 x 5 g *Salicylate #3 Reagent Powder *7458-C

1 Spoon, 0.1 g, plastic 0699

1 Spoon, 0.15 g, plastic 0727

1 Pipet, 1.0 mL, plastic 0354

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Ammonia nitrogen is present in various concentrations in many surface and ground water supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply is cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes accepted as chemical evidence of pollution when encountered in natural waters.

Ammonia is rapidly oxidized in natural water systems by special bacterial groups that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in the water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to the expanded growth of undesirable algae and other forms of plant growth that overload the natural system and cause pollution.

Test Procedures

APPLICATION: Low concentrations of ammonia in fresh, brackish and salt

water; fresh and salt water aquariums.

RANGE: 0.00 - 1.00 ppm Ammonia-Nitrogen

MDL: 0.05 ppm Fresh Water

0.10 ppm Salt Water

METHOD: Salicylate and ammonia react at high pH in the presence

of a chlorine donor and an iron catalyst to form a blue indophenol dye, the concentration of which is proportional to the ammonia concentration in the sample.

SAMPLE HANDLE & PRESERVATION:

INTERFERENCES: There are few interferences in most natural waters. High

SMART3 Test Procedures 11.10 AMMONIA NITROGEN, Low Range

Ammonia solutions tend to be unstable and should be analyzed immediately. Samples may be stored for 24 hours at 4°C or 28 days at –20°C.

concentrations of reducing agents, such as hydrazine, react with the chlorine donor and can result in negative interferences. Color and turbidity can also interfere.

PROCEDURE - FRESH WATER

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 003 Ammonia-N LRF)

4. Scroll to and select 003 Ammonia-N LRF from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)

7. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.0

8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent

9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures

10. At the end of the 12 minute waiting period, immediately mix and insert tube into

11. Press

CALCULATIONS:

ENTER

to select TESTING MENU.

from TESTING MENU.

mL of *Salicylate Ammonia #1 (3978). Cap and mix.

(7457). Cap and mix until dissolved. Wait 1 minute.

of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least 30 seconds and all solid has dissolved. Wait 12 minutes for maximum color development.

chamber, close lid and select SCAN SAMPLE. Record result.

to turn colorimeter off or press

EXIT

to exit to a previous menu or

make another menu selection.

To express results as Unionized Ammonia (NH3):

ppm Unionized Ammonia (NH3) =

ppm Ammonia-Nitrogen (NH3–N) x 1.2

Test Procedures

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

+

) =

4

ppm Ammonia-Nitrogen (NH3–N) x 1.3

To determine the percentages of Unionized and Ionized Ammonia-Nitrogen, consult the Appendix.

NOTE: It is strongly suggested that a reagent blank be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

PROCEDURE - SALT WATER

AMMONIA NITROGEN, Low Range SMART3 Test Procedures 11.10

1. Press and hold until colorimeter turns on.

2. Press

ENTER

to select TESTING MENU.

3. Select ALL TESTS (or another sequence containing 004 Ammonia-N LRS) from TESTING MENU.

4. Scroll to and select 004 Ammonia-N LRS from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.)

7. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL of *Salicylate Ammonia #1 (3978). Cap and mix.

8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent (7457). Cap and mix until dissolved. Wait 1 minute.

9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least 30 seconds and all solid has dissolved. Wait 20 minutes for maximum color development.

10. At the end of the 20 minute waiting period, immediately mix and insert tube into chamber, close lid and select SCAN SAMPLE. Record result.

11. Press to turn colorimeter off or press

EXIT

to exit to a previous menu or

make another menu selection.

CALCULATIONS:

To express results as Unionized Ammonia (NH3):

Test Procedures

ppm Unionized Ammonia (NH3) =

ppm Ammonia-Nitrogen (NH3–N) x 1.2

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

4

+

) =

ppm Ammonia-Nitrogen (NH3–N) x 1.3

To determine the percentages of Unionized and Ionized Ammonia-Nitrogen, consult the Appendix.

NOTE: It is strongly suggested that a reagent blank be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

SMART3 Test Procedures 11.10 AMMONIA NITROGEN, Low Range

AMMONIA NITROGEN - HIGH RANGE

NESSLERIZATION METHOD •CODE 3642-SC

QUANTITY CONTENTS CODE

30 mL Ammonia Nitrogen Reagent #1 V-4797-G

2 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G

1 Pipet, 1 mL, plastic 0354

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Ammonia nitrogen is present in various concentrations in many surface and ground water supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply is cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes accepted as chemical evidence of pollution when encountered in natural waters.

Ammonia is rapidly oxidized in natural water systems by special bacterial groups that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in the water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to the expanded growth of undesirable algae and other forms of plant growth that overload the natural system and cause pollution.

APPLICATION: Drinking, surface, and saline waters; domestic and

industrial wastes.

RANGE: 0.00–4.00 ppm Ammonia Nitrogen

MDL: 0.05 ppm

Test Procedures

METHOD: Ammonia forms a colored complex with Nessler’s

Reagent in proportion to the amount of ammonia present in the sample. Rochelle salt is added to prevent precipitation of calcium or magnesium in undistilled samples.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Sample turbidity and color may interfere. Turbidity

Ammonia solutions tend to be unstable and should be analyzed immediately. Sample may be stored for 24 hours at 4°C or 28 days at –20°C.

may be removed by a ﬁ ltration procedure. Color interference may be eliminated by blanking the instrument with a sample blank.

AMMONIA NITROGEN, Low Range SMART3 Test Procedures 11.10

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Scroll to and select ALL TESTS (or another sequence containing 005

4. Scroll to and select 005 Ammonia-N HR from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note)

7. Remove tube from colorimeter. Add 8 drops of Ammonia Nitrogen Reagent #1

8. Use the 1.0 mL pipet (0354) to add 1.0 mL of *Ammonia Nitrogen Reagent #2

9. At end of the 5 minute waiting period, immediately mix, insert tube into

10. Press

CALCULATIONS:

ENTER

to select TESTING MENU.

Ammonia-N HR) from TESTING MENU.

(V-4797). Cap and mix. Wait 1 minute.

(V-4798). Cap and mix. Allow 5 minutes for maximum color development.

chamber, close lid and select SCAN SAMPLE. Record result.

to turn the colorimeter off or press the

EXIT

exit to a previous

menu or make another menu selection.

To express results as Unionized Ammonia (NH3):

ppm Unionized Ammonia (NH3) =

ppm Ammonia-Nitrogen (NH3–N) x 1.2

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

4

+

) =

ppm Ammonia-Nitrogen (NH3–N) x 1.3

To determine the percentages of Unionized and Ionized Ammonia-Nitrogen, consult the Appendix.

NOTE: It is strongly suggested that a reagent blank be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

SMART3 Test Procedures 11.10 AMMONIA NITROGEN, High Range

Test Procedures

AMMONIA NITROGEN, High Range SMART3 Test Procedures 11.10

BARIUM

BARIUM CHLORIDE METHOD • CODE 3638-SC

QUANTITY CONTENTS CODE

50 g Barium Reagent Powder 6330-H

1 Spoon, 0.5g, plastic 0698

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Barium is a naturally occurring metal that is found in rocks and, in trace amounts, in natural waters. A barium concentration above 2 ppm in drinking water is a classiﬁ ed as a contaminant. Barium sulfate is used in the medical ﬁ eld as an X-ray radio contrast agent for imaging the gastrointestinal tract. Barium has many industrial applications. It is used in the manufacture of paints, brakes, root canal ﬁ llings, glass, and motor oil detergents. Barium nitrate imparts a bright yellow-green color to ﬁ reworks and ﬂ ares. Barium sulfate is a component of oil well drilling mud which is used to lubricate drill bits. Barium is also a constituent of some electro ceramics and high temperature yttrium barium copper oxide (YBCO) superconductors.

APPLICATION: Industrial

RANGE: 0 – 200 ppm barium

MDL: 5 ppm

METHOD: Barium is precipitated in an acid medium with

sodium sulfate to form a barium sulfate suspension in proportion to the amount of barium present.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Suspended matter and color interference may be

SMART3 Test Procedures 02.12 BARIUM

Barium samples may be preserved by refrigeration at 4°C up to 7 days in glass or plastic containers without any change in concentration.

removed by a ﬁ ltration step. Silica in excess of 500 mg/L will interfere. Check for stray light interference (see page 69).

Test Procedures

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 018 Barium) from TESTING

4. Scroll to and select 018 Barium from menu.

5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert the tube into chamber, close the lid and select SCAN BLANK.

7. Remove the tube from the colorimeter.

8. Use the 0.5 g spoon (0698) to add one measure of Barium Reagent Powder

9. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result in

10. Press

NOTE: A while ﬁ lm is deposited on the inside of test tubes as a result of the barium test. Thoroughly clean and rinse test tubes after each test.

ENTER

to select Testing Menu.

MENU.

(6330). Cap and mix by inverting for one minute.

ppm barium.

to turn the colorimeter off or press

or make another menu selection.

EXIT

to exit to a previous menu

For the most accurate results, samples and reactions should be at 25 ±4°C.

Test Procedures

BARIUM SMART3 Test Procedures 02.12

BENZOTRIAZOLE/TOLYLTRIAZOLE

UV Photolysis Method • CODE 4047

QUANTITY CONTENTS CODE

15 g *Benzotriazole Reagent *3818-E

25 mL Potassium Sodium Tartrate Solution 7841WT-G

25 mL *Sulfuric Acid *6139WT-G

1 pH Test Papers, 1–11 2956

1 Spoon, 0.25 g, plastic 0695

1 Erlenmeyer Flask, 25 mL, glass 2-2109

1 Graduated Cylinder, 25 mL, glass 0417

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Equipment needed but not supplied:

1 UV Shielding Goggles 31041

1 Pen-Ray UV Lamp 31041-1

1 Pen-Ray Lamp Power Source 31041-2

Test Procedures

Proper safety precautions must be followed when using the Pen-Ray UV lamp and power source (31041-1 and 31041-2) to prevent eye and skin damage. Always wear the UV Shielding Goggles (31041) while the lamp is turned on. Never handle the lamp itself; always hold it by the socket. Wipe the lamp dry with a clean, soft tissue after each test. Do not operate the lamp outside the Erlenmeyer Flask ﬁ lled with water.

Benzotriazole and tolyltriazole form strong complexes with metals. They are used in antifreeze for cars, lubricating oil, and photographic anti-fogging agents. In cooling water systems benzotriazole and tolyltriazole are used as corrosion and rust inhibitors together with many kinds of scale inhibitors, bactericides and algaecides.

SMART3 Test Procedures 11.10 BENZOTRIAZOLE/TOLYTRIAZOLE

APPLICATION: Corrosion and rust inhibitors in cooling water systems

RANGE: 0.0 – 30.0 ppm Benzotriazole

0.0 – 30.0 ppm Tolyltriazole

MDL: 0.5 ppm Benzotriazole

0.5 ppm Tolyltriazole

METHOD: Benzotriazole and tolyltriazole are UV-photolyzed in a

buffered solution with a pH between 4 and 6. A yellow color develops in proportion to the concentration of triazole present.

SAMPLE HANDLING &

Samples should be analyzed as soon as possible after collection.

PRESERVATION:

INTERFERENCES: Tolyltriazole with interfere in the benzotriazole test.

Benzotriazole will interfere in the tolyltriazole test. Strong reducing or oxidizing agents will interfere.

Test Procedures

BENZOTRIAZOLE/TOLYTRIAZOLE SMART3 Test Procedures 11.10

BENZOTRIAZOLE PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 009 Benzotriazole)from

4. Scroll to and select 009 Benzotriazole from menu.

5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.

6. Insert the tube into chamber, close lid and select SCAN BLANK.

7. Remove the tube from colorimeter. Discard the sample.

8. Adjust the sample water temperature to between 20 and 25°C if necessary.

9. Fill the graduated cylinder (0417) to the 25 mL line with sample water. Transfer

10. Use the pH Test Paper (2956) to check the pH of the sample. If the pH is not

11. Add 10 drops of Potassium Sodium Tartrate (7841WT).

12. Use the 0.25 g spoon (0695) to add one measure of *Benzotriazole Reagent

13. Replace the ﬂ ask in the slot in the case. Insert the Pen-Ray Lamp (31041-1)

14. Fill a test tube (0290) to the 10 mL line with the digested sample. Cap tube.

15. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result in

16. Press

NOTE: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

ENTER

to select Testing Menu.

TESTING MENU.

to the Erlenmeyer Flask (2-2109).

between 4 and 6, add one drop of *Sulfuric Acid, 1.0N (6139). Swirl to mix. Continue adding *Sulfuric Acid, 1.0N (6139) one drop at a time, swirling to mix and checking the pH after each drop, until the pH is between 4 and 6.

(3818). Swirl to mix until the powder has dissolved.

into the ﬂ ask. Plug in the Pen-Ray Power Source (31041-2) and turn the lamp on for exactly 5 minutes. Remove the lamp from the ﬂ ask. Rinse and wipe the lamp dry.

ppm Benzotriazole.

to turn the colorimeter off or press

EXIT

to exit to a previous

menu or make another menu selection.

Test Procedures

SMART3 Test Procedures 11.10 BENZOTRIAZOLE/TOLYTRIAZOLE

TOLYLTRIAZOLE PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 097 Tolyltriazole) from

4. Scroll to and select 097 Tolyltriazole from menu.

5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.

6. Insert the tube into chamber, close lid and select SCAN BLANK.

7. Remove the tube from colorimeter. Discard the sample.

8. Adjust the sample water temperature to between 20 and 25°C if necessary.

9. Fill the graduated cylinder (0417) to the 25 mL line with sample water. Transfer

10. Use the pH Test Paper (2956) to check the pH of the sample. If the pH is not

11. Add 10 drops of Potassium Sodium Tartrate (7841WT).

12. Use the 0.25 g spoon (0695) to add one measure of *Benzotriazole Reagent

13. Replace the ﬂ ask in the slot in the case. Insert the Pen-Ray Lamp (31041-1)

14. Fill a test tube (0290) to the 10 mL line with the digested sample. Cap tube.

15. Insert tube into chamber, close lid and select SCAN SAMPLE. Record result in

Test Procedures

16. Press

NOTE: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

ENTER

to select Testing Menu.

TESTING MENU.

to the Erlenmeyer Flask (2-2109).

between 4 and 6, add one drop of *Sulfuric Acid, 1.0N (6139). Swirl to mix. Continue adding *Sulfuric Acid, 1.0N (6139) one drop at a time, swirling to mix and checking the pH after each drop, until the pH is between 4 and 6.

(3818). Swirl to mix until the powder has dissolved.

into the ﬂ ask. Plug in the Pen-Ray Power Source (31041-2) and turn the lamp on for exactly 5 minutes. Remove the lamp from the ﬂ ask. Rinse and wipe the lamp dry.

ppm Tolyltriazole.

to turn the colorimeter off or press

EXIT

to exit to a previous

menu or make another menu selection.

BENZOTRIAZOLE/TOLYTRIAZOLE SMART3 Test Procedures 11.10

BIGUANIDE

COLORIMETRIC METHOD • CODE 4044

QUANTITY CONTENTS CODE

2 X 60 mL Biguanide Indicator 3994-H

1 Pipet, plastic, 1.0 mL 0354

Biguanide is a non-chlorine, non-bromine chemical sanitizer. It is more stable than chlorine or bromine and has little chemical odor. Biguanide is an effective bacteriacide but, unlike chlorine and bromine, it does not destroy organic contaminants. Therefore, hydrogen peroxide is added to biguanide pools on a regular basis to eliminate organic contaminants. The optimum recommended level of biguanide is 30 to 50 ppm.

APPLICATION: Swimming pools

RANGE: 0–70 ppm Biguanide

MDL: 2 ppm

METHOD: Biguanide complexes with the proprietary indicator to

produce a colored solution. The color ranges from yellow through green to blue depending on the biguanide concentration.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: The only interfering substances that are likely to be

Samples should be analyzed as soon as possible.

encountered in pool water are oxidized manganese and oxidizing agents, such as chlorine, bromine and ozone.

Test Procedures

SMART3 Test Procedures 11.10 BIGUANIDE

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 006 Biguanide) from

4. Scroll to and select 006 Biguanide from menu.

5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.

6. Insert the tube into chamber, close lid and select SCAN BLANK.

7. Remove the tube from colorimeter.

8. Use the 1.0 mL pipet (0354) to add 2.0 mL of Biguanide Indicator (3994). Cap

9. Wait 1 minute.

10. Insert the tube into chamber. Close lid.

11. Select SCAN SAMPLE. Record result in ppm Biguanide

12. Press

NOTE: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

Test Procedures

ENTER

to select Testing Menu.

TESTING MENU.

and invert three times to mix.

to turn the colorimeter off or press

menu or make another menu selection.

EXIT

to exit to a previous

BIGUANIDE SMART3 Test Procedures 11.10

BORATE - UDV

UNIT DOSE VIALS • CODE 4322-J

QUANTITY CONTENTS CODE

50 Borate UDV, 20 pouches 4322-J

Equipment needed but not supplied:

STANDARD ACCESSORY PACKAGE · CODE 1961

1 Package of 3 Vials (empty) 0156

1 Syringe, 3 mL, plastic 1184

1 Foil Storage Bag 9467

Or:

ADVANCED ACCESSORY PACKAGE · CODE 1962

1 Pipettor, 3mL 30528

1 Pipet Tip (0-5 mL) 30695

1 Cuvette Rack 31695

1 Package of 3 Vials (empty) 0156

1 Foil Storage Bag 9467

Test Procedures

Some swimming pools use a borate buffering system. Borates lower the level of carbon dioxide in the pool, which slows algae growth. This results in a lower chlorine requirement. Free chlorine levels in pools with borate systems can be maintained at 1.0 ppm.

Small amounts of boron are necessary for plant growth but large amounts can be toxic. In humans, boron aids in the uptake of calcium and the production of strong bones. An excess of boron can affect the central nervous system resulting in a syndrome known as borism. Some natural waters may contain small amounts of boron. Large concentrations may be due to industrial efﬂ uent entering waterways. Boron compounds are used in cleaning compounds, paper and paints, fertilizers, glass and ceramics, ﬁ re retardants and the production of alloys. In the atomic energy ﬁ eld, boron is a component of neutron shields and nuclear reactors.

SMART3 Test Procedures 11.10 BORATE, UDV

APPLICATION: swimming pools, surface and saline waters, hydroponic

solutions, industrial waste.

RANGE: 0.00 – 80.0 ppm boron

MDL: 5 ppm

METHOD: Borate reacts with a selective boron indicator powder to

form a colored complex at pH 7.2 to 7.4 in proportion to the concentration of boron present.

SAMPLE HANDLING & PRESERVATION:

Store samples in polyethylene bottles. Do not use borate detergents or glassware.

INTERFERENCES: Interferences in swimming pool water are unlikely.

Test Procedures

BORATE, UDV SMART3 Test Procedures 11.10

PROCEDURE

Use COD/UDV adapter

1. Press until colorimeter turns on.

2. Press

3. Select ALL TESTS (or another sequence containing 007 Borate UDV) from

4. Scroll to and select 007 Borate UDV from menu.

5. Rinse a clean vial (0156) with sample water.

6. Use the syringe (1184) to add 3mL of sample to the vial.

7. Insert the vial into chamber, close the lid and select SCAN BLANK.

8. Remove the vial from the colorimeter.

9. Use the syringe (1184) to add 3mL of sample to a Borate UDV vial (4322).

10. Wait 3 minute.

11. Invert the vial three times to mix.

12. Immediately insert tube into chamber, close lid and select SCAN SAMPLE.

13. Press

NOTE: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents are obtained.

ENTER

to select Testing Menu.

TESTING MENU.

NOTE: If powder residue remains in the bottom of the vial after inverting or air bubbles form, invert once more and tap bottom of vial sharply once or twice to dislodge powder and bubbles. Mix.

Record result in ppm borate.

to turn the colorimeter off or press

EXIT

to exit to a previous

menu or make another menu selection.

Test Procedures

NOTE: UDVs from opened pouches should be used promptly. Store unused vials from opened pouches in the Foil Storage Bag (9467) to extend the shelf life of the reagent. Generally, UDVs stored in the bag should be used within 10 days if the humidity is less than 50% and within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced.

SMART3 Test Procedures 11.10 BORATE, UDV

Test Procedures

BORATE, UDV SMART3 Test Procedures 11.10

BORON

AZOMETHINE-H METHOD · CODE 4868-01

QUANTITY CONTENTS CODE

120 mL *Boron Buffer *4869-J

10 g *Boron Indicator Powder *4870-D

1 Pipet, plastic, 1.0 mL 0354

1 Spoon, 0.15 g 0727

1 Dark storage chamber, brown 0108

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

Small amounts of boron are necessary for plant growth but large amounts can be toxic. In humans, boron aids in the uptake of calcium and the production of strong bones. An excess of boron can affect the central nervous system resulting in a syndrome known as borism. Some natural waters may contain small amounts of boron. Large concentrations may be due to industrial efﬂ uent entering waterways. Boron compounds are used in cleaning compounds, paper and paints, fertilizers, glass and ceramics, ﬁ re retardants and the production of alloys. In the atomic energy ﬁ eld, boron is a component of neutron shields and nuclear reactors. Some swimming pools use boron buffering systems.

Test Procedures

APPLICATION: Surface and saline waters, hydroponic solutions, industrial

waste, swimming pools.

RANGE: 0.00–0.80 ppm Boron

MDL: 0.05

METHOD: Azomethine-H and borate form a yellow complex at pH 6

in proportion to the concentration of boron present.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Interferences in drinking water are unlikely. Manganese,

SMART3 Test Procedures 2.11 BORON

Store samples in polyethylene bottles. Do not use borate detergents or glassware.

zirconium, chromium, titanium, copper, vanadium, aluminum, beryllium and iron may cause high results.

PROCEDURE

1. This test requires a Reagent Blank. Rinse a tube (0290) with clear, colorless, boron free water. Fill to 10 mL line with clear, colorless, boron free water.

2. Use the 1.0 mL pipet (0354) to add 2 mL of *Boron Buffer (4869). Cap and mix.

3. Use the 0.15 g spoon (0727) to add one level measure of *Boron Indicator Powder (4870). Press full spoon against side of jar to compress powder. Scrape off excess powder on inside neck of bottle. Tap excess off spoon handle.

4. Cap and shake vigorously for 30 seconds.

5. Insert the tube into meter chamber. Close lid.

6. Start a timer set for 30 minutes. Do not open the lid during the waiting time. The reaction is photosensitive.

7. Rinse a clean tube (0290) with Sample Water. Fill to the 10 mL line with sample water. Repeat steps 2–4.

8. Insert the tube into the Dark Storage Chamber (0108). Close top.

9. Start a second timer set for 30 minutes. Do not open the chamber during the waiting time. The reaction is photosensitive.

10. When 2 minutes remain on the ﬁ rst timer (Reagent Blank), press and hold ON button until colorimeter turns on.

11. Press and hold

12. Press

ENTER

to select TESTING MENU.

13. Select ALL TESTS (or another sequence containing 008 Boron) from TESTING MENU.

14. Scroll to and select 008 Boron from menu.At the end of the Reagent Blank 30 minute waiting period, remove Reagent Blank tube from meter chamber. Invert several times to mix.

Test Procedures

15. Insert the tube into meter chamber, close lid and select SCAN BLANK.

16. Remove the tube from colorimeter.

17. At the end of the Sample Water 30 minute waiting period, remove Sample Water tube from Dark Storage Chamber. Invert several times to mix.

18. Insert tube into meter chamber, close lid and select SCAN SAMPLE. Record result in ppm boron.

19. Press to turn colorimeter off or press the or make another menu selection.

until colorimeter turns on.

EXIT

to exit to a previous menu

BORON SMART3 Test Procedures 2.11

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