Lamotte SMART3 Colorimeter User Manual

SMART3

Colorimeter

Operator’s

Manual

Warning! This set contains chemicals that may be harmful if misused. Read cautions on individual containers carefully. Not to be used by children except under adult supervision.

1910-MN-V3 06.28.19

1910-MN-V3.indd

PO Box 329 • Chestertown, MD 21620 800-344-3100 • f 410-778-6394 www.lamotte.com

CONTENTS

GENERAL INFORMATION

 Packaging & Delivery ...................................................................................................................................5

 General Precautions .................................................................................................................................. 5

 Safety Precautions ......................................................................................................................................5

 Limits of Liability ......................................................................................................................................... 6

 Warranty .........................................................................................................................................................6

 Register Your Meter......................................................................................................................................6

 Speciﬁcations ............................................................................................................................................. 7

 Statistical and Technical Deﬁnitions ......................................................................................................9

 Contents and Accessories ...................................................................................................................... 10

 EPA Compliance ........................................................................................................................................11

 CE Compliance ........................................................................................................................................... 11

 IP 67 Certiﬁcation ...................................................................................................................................... 11

CHEMICAL TESTING

 Water Sampling for Chemical Analysis ...............................................................................................12

 Filtration ..................................................................................................................................................... 13

 An Introduction to Colorimetric Analysis .......................................................................................... 14

 Reagent Blank .......................................................................................................................................... 15

 Colorimeter Tubes & Chamber ............................................................................................................. 15

 Meter Care .................................................................................................................................................. 16

 Selecting an Appropriate Wavelength ............................................................................................... 16

 Calibration................................................................................................................................................... 16

 Calibration Curves ................................................................................................................................... 16

 Standard Additions ................................................................................................................................. 19

 Sample Dilution & Volumetric Measurements ................................................................................ 19

 Interferences ............................................................................................................................................ 20

 Stray Light Interference ......................................................................................................................... 20

OPERATION OF THE SMART3 COLORIMETER

 Overview ..................................................................................................................................................... 21

 Components .............................................................................................................................................. 22

GENERAL OPERATING PROCEDURES

 The Keypad ................................................................................................................................................ 23

 Sample Holders ......................................................................................................................................... 24

 The Display & the Menus ....................................................................................................................... 24

TESTING

 Testing Menu ............................................................................................................................................. 26

 Test Sequences ........................................................................................................................................ 27

 General Testing Procedures .................................................................................................................. 27

 Testing With LaMotte Pre-Programmed Tests ................................................................................ 28

 Calibrating LaMotte Pre-Progammed Tests ..................................................................................... 30

 Measuring in the Absorbance Mode .................................................................................................... 33

EDITING MENU

 Editing a Sequence .................................................................................................................................. 36

 Adding or Deleting a Test ....................................................................................................................... 38

 Edit User Tests .......................................................................................................................................... 42

 Naming the Test ....................................................................................................................................... 44

 Selecting the Vial and Wavelength ..................................................................................................... 47

 Entering a Two Point Calibration ......................................................................................................... 48

 Entering a Multiple Point Calibration....................................................................................................51

 Selecting the Numerical Format of the Result .................................................................................54

 Selecting Units of Concentration ......................................................................................................... 55

 Setting the Clock ....................................................................................................................................... 56

 Logging Data .............................................................................................................................................. 57

 Factory Setup ............................................................................................................................................ 58

 Setting Power Save .................................................................................................................................. 58

 Setting Backlight Time ............................................................................................................................. 59

 Bluetooth Menu .......................................................................................................................................... 60

 Selecting a Language ............................................................................................................................... 67

 Looping Menu .............................................................................................................................................69

COMPUTER CONNECTION

 Output ......................................................................................................................................................... 70

 Computer Connection ............................................................................................................................. 70

 Bluetooth Connection ............................................................................................................................... 70

BATTERY

 Battery/AC Operation ................................................................................................................................71

 Battery Replacement ................................................................................................................................ 71

MAINTENANCE

 Cleaning ...................................................................................................................................................... 73

 Repairs ......................................................................................................................................................... 73

 Meter Disposal ........................................................................................................................................... 73

TROUBLESHOOTING

 Error Messages .......................................................................................................................................... 74

 Troubleshooting Guide .............................................................................................................................75

 Software Troubleshooting Guide .......................................................................................................... 75

SMART3 COLORIMETER TEST PROCEDURES APPENDIX

GENERAL INFORMATION

 PACKAGING & DELIVERY

Experienced packaging personnel at LaMotte Company assure adequate protection against normal hazards encountered in transportation of shipments. After the product leaves the manufacturer, all responsibility for its safe delivery is assured by the transportation company. Damage claims must be ﬁled immediately with the transportation company to receive compensation for damaged goods.

Should it be necessary to return the instrument for repair or servicing, pack instrument carefully in a suitable container with adequate packing material. A return authorization number must be obtained from LaMotte Company by calling 1-800-344-3100 or emailing tech@lamotte.com. Attach a letter with the authorization number to the shipping carton which describes the kind of trouble experienced. This valuable information will enable the service department to make the required repairs more eﬃciently.

 GENERAL PRECAUTIONS

Before attempting to set up or operate this instrument it is important to read the instruction manual. Failure to do so could result in personal injury or damage to the equipment.

The Smart3 Colorimeter should not be stored or used in a wet or corrosive environment. Care should be taken to prevent water or reagent chemicals from wet colorimeter tubes from entering the colorimeter chamber.

NEVER PUT WET TUBES IN COLORIMETER.

 SAFETY PRECAUTIONS

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800255-3924) (International, call collect, 813-248-0585)

To order individual reagents or test kit components, use the speciﬁed code number. Keep equipment and reagent chemicals out of the reach of young children. Ensure that the protection provided by this equipment is not impaired. Do not intall or use

this equipment in a manner that is not indicated in this manual.

 LIMITS OF LIABILITY

Under no circumstances shall LaMotte Company be liable for loss of life, property, proﬁts, or other damages incurred through the use or misuse of its products.

 WARRANTY

LaMotte Company warrants this instrument to be free of defects in parts and workmanship for 2 years from the date of shipment. If it should become necessary to return the instrument for service during or beyond the warranty period, contact our Technical Service Department at 1-800-344-3100 or tech@lamotte.com for a return authorization

SMART3 Colorimeter 09.16 5

number or visit www.lamotte.com for troubleshooting help. The sender is responsible for shipping charges, freight, insurance and proper packaging to prevent damage in transit. This warranty does not apply to defects resulting from action of the user such as misuse, improper wiring, operation outside of speciﬁcation, improper maintenance or repair, or unauthorized modiﬁcation. LaMotte Company speciﬁcally disclaims any implied warranties or merchantability or ﬁtness for a speciﬁc purpose and will not be liable for any direct, indirect, incidental or consequential damages. LaMotte Company’s total liability is limited to repair or replacement of the product. The warranty set forth above is inclusive and no other warranty, whether written or oral, is expressed or implied.

 REGISTER YOUR METER

To register your meter with the LaMotte Service Department, go to www.lamotte.com and choose SUPPORT on the top navigation bar.

 SPECIFICATIONS

INSTRUMENT TYPE: Colorimeter

Readout 160 x 100 backlit LCD, 20 x 6 line graphical display

Wavelengths 428 nm, 525 nm, 568 nm, 635 nm

Wavelength Accuracy ±2% FS

Readable Resolution Determined by reagent system

Wavelength Bandwidth 10 nm typical

Photometric Range –2 to +2 AU

Photometric Precision ± 0.001 AU at 1.0 AU

Photometric Accuracy ±0.005 AU at 1.0 AU

Sample Chamber Accepts 25 mm diameter ﬂat-bottomed test tubes, 10 mm

square cuvettes†, 16 mm COD test tubes

Light Sources 4 LEDs

Detectors 4 silicon photodiodes

Modes Pre-programmed tests, absorbance, %T

Pre-Programmed Tests YES, with automatic wavelength selection

User Deﬁned Tests Up to 25 user tests can be input

Languages English, Spanish, French, Portuguese, Italian, Chinese,

Japanese

Temperature Operation: 0-50 °C; Storage: -40-60 °C

Operation Humidity Range

USB Port Mini B

Power Requirements USB wall adapter, USB computer connection or lithium ion

0-90 % RH, non-condensing

rechargeable battery

6 SMART3 Colorimeter 09.16

Battery Charge Life: Approximately 380 tests with backlight on to

1000 tests with backlight oﬀ. (Signal averaging disabled). Battery Life: Approximately 500 charges.

Electrical Rating Rated voltage (5V), Rated power of input current (1.0A) at

mini-USB input port

Data Logger 500 test results stored for download to a PC

Waterproof IP67 with USB port plug in place

Certiﬁcations

BT Radio FCC ID:

Cert# BQB

LISTED

CE Mark Safety

EMC

T9J-RN42 6514A-RN42 B014867-SPP and DUN

proﬁles

EN61610-1:2010 +Corrigendum 1:2011

EN 301 489-17 and EN 301 489

US EMC FCC CFR 47, Part 15,

subpart B

Canada EMC CAN ICES-3(A)/NMB-3(A)

AU/NZ EMC AS/NZS CISPR 22

Dimensions (LxWxH) 3.5 x 7.5 x 2.5 inches, 8.84 x 19.05 x 6.35 cm

Weight 13 oz, 362 g (meter only)

†525 nm and 568 nm only

 STATISTICAL & TECHNICAL DEFINITIONS RELATED TO

PRODUCT SPECIFICATIONS

Method Detection Limit (MDL): “The method detection limit (MDL) is deﬁned as the

minimum concentration of a substance that can be measured and reported with 99% conﬁdence that the analyte concentration is greater than zero and is determined from analysis of a sample in a given matrix containing the analyte.”1 Note that, “As Dr. William Horwitz once stated, ‘In almost all cases when dealing with a limit of detection or limit of determination, the primary purpose of determining that limit is to stay away from it.’”

Accuracy: Accuracy is the nearness of a measurement to the accepted or true value.3 The accuracy can be expressed as a range, about the true value, in which a measurement occurs (i.e. ±0.5 ppm). It can also be expressed as the % recovery of a known amount of analyte in a determination of the analyte (i.e. 103.5 %).

Resolution: Resolution is the smallest discernible diﬀerence between any two measurements that can be made.4 For meters this is usually how many decimal places are displayed. (i.e. 0.01). Note that the resolution many change with concentration or range. In some cases the resolution may be less than the smallest interval, if it is possible to make a reading that falls between calibration marks. A word of caution, that resolution has very little relationship to accuracy or precision. The resolution will always be less than the accuracy or precision but it is not a statistical measure of how well a method of analysis

SMART3 Colorimeter 09.16 7

2

works. The resolution can be very, very good and the accuracy and precision can be very bad! This is not a useful measure of the performance of a test method.

Repeatability: Repeatability is the within-run precision.5 A run is a single data set, from set up to clean up. Generally, one run occurs on one day. However, for meter calibrations, a single calibration is considered a single run or data set, even though it may take 2 or 3 days.

Reproducibility: Reproducibility is the between-run precision.

6

Detection Limit (DL): The detection limit (DL) for the 2020we/wi is deﬁned as the minimum value or concentration that can be determined by the meter, which is greater than zero, independent of matrix, glassware, and other sample handling sources of error. It is the detection limit for the optical system of the meter.

1

CFR 40, part 136, appendix B

2

Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta, American

Laboratory, Sept 2003, P. 31.

3

Skoog, D.A., West, D. M., Fundamental of Analytical Chemistry, 2nd ed., Holt Rinehart and

Winston, Inc, 1969, p. 26.

4

Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta, American

Laboratory, Sept 2003, P. 34.

5

Jeﬀery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative

Chemical Analysis, 5th ed., Longman Scientiﬁc & Technical, 1989, p. 130.

6

Jeﬀery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative

Chemical Analysis, 5th ed., Longman Scientiﬁc & Technical, 1989, p. 130

 CONTENTS AND ACCESSORIES

Contents

Smart3 Colorimeter

Test Tubes, with Caps Code 0290

COD/UDV Adapter Code 1724

USB Wall Adapter Code 1721

USB Cable Code 1720-01

Smart3 Colorimeter Quick Start Guide

Smart3 Colorimeter Manual

Accessories

Test Tubes, with Caps Code 0290-6

Replacement Chamber Code 3-0038

USB Cable Code 1720-01

8 SMART3 Colorimeter 09.16

3.17

USB Wall Adapter Code 1721

COD/UDV Adapter Code 1724

Bluetooth Mobile Printer Code 3-0066

Car Charger Code 5-0132

Small Field Carrying Case (37.5 27.5 x 13.75 cm) Code 1910-GCS150

Large Field Carrying Case (45 x 32.5 x 20 cm) Code 1910-GCS440

WARNING: Only use the USB cable (1720-01) that is supplied with the kit. Make no substitutions.

 EPA COMPLIANCE

The Smart3 Colorimeter is an EPA-Accepted instrument. EPA-Accepted means that the instrument meets the requirements for instrumentation as found in test procedures that are approved for the National Primary Drinking Water Regulations (NPDWR) or National Pollutant Discharge Elimination System (NPDES) compliance monitoring programs. EPAAccepted instruments may be used with approved test procedures without additional approval.

 CE COMPLIANCE

The Smart3 Colorimeter has earned the European CE Mark of Compliance for electromagnetic compatibility and safety. The Declaration of Conformity for the Smart3 colorimeter is available at www.lamotte.com.

This device complies with Part 15 of the FCC rules. Operation is subject to the following two conditions (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interferences in which case the user will be required to correct the interference at his own expense.

operated in a

 IP67 CERTIFICATION

The Smart3 meets IP67 standards for protection against dust and immersion only when the USB port plug is in place. Documentation is available at www.lamotte.com.

SMART3 Colorimeter 09.16 9

CHEMICAL TESTING

 WATER SAMPLING FOR CHEMICAL ANALYSIS

Taking Representative Samples

The underlying factor to be considered for any type of water sampling is whether or not the sample is truly representative of the source. To properly collect a representative sample:

◊ Sample as frequently as possible. ◊ Collect a large sample or at least enough to conduct whatever tests are necessary. ◊ Make a composite sample for the same sampling area. ◊ Handle the sample in such a way as to prevent deterioration or contamination before

the analysis is performed.

◊ Perform analysis for dissolved gases such as dissolved oxygen, carbon dioxide, and

hydrogen sulﬁde immediately at the site of sampling. Samples for testing these factors, as well as samples for pH, cannot be stored for later examination.

◊ Make a list of conditions or observations which may aﬀect the sample. Other

considerations for taking representative samples are dependent upon the source of the sample. Taking samples from surface waters involves diﬀerent considerations than taking samples from impounded and sub-surface waters.

Sampling of Open Water Systems

Surface waters, such as those found in streams and rivers, are usually well mixed. The sample should be taken downstream from any tributary, industrial or sewage pollution source. For comparison purposes samples may be taken upstream and at the source of the pollution before mixing.

In ponds, lakes, and reservoirs with restricted ﬂow, it is necessary to collect a number of samples in a cross section of the body of water, and where possible composite samples should be made to ensure representative samples.

To collect samples from surface waters, select a suitable plastic container with a tight ﬁtting screw cap. Rinse the container several times with the sample to be tested, then immerse the container below the surface until it is ﬁlled to overﬂowing and replace the cap. If the sample is not to be tested immediately, pour a small part of the sample out and reseal. This will allow for any expansion. Any condition which might aﬀect the sample should be listed.

Sub-surface sampling is required to obtain a vertical proﬁle of streams, lakes, ponds, and reservoirs at speciﬁc depths. This type of sampling requires more sophisticated sampling equipment.

For dissolved oxygen studies, or for tests requiring small sample sizes, a Water Sampler (LaMotte Code 1060) will serve as a subsurface or in-depth sampler. This weighted device is lowered to the sampling depth and allowed to rest at this depth for a few minutes. The water percolates into the sample chamber displacing the air which bubbles to the surface. When the bubbles cease to rise, the device has ﬂushed itself approximately ﬁve times and it may be raised to the surface for examination. The inner chamber of the sampling device is lifted out and portions of the water sample are carefully dispensed for subsequent chemical analysis.

10 SMART3 Colorimeter 09.16

A Snap-Plunger Water Sampler (LaMotte Code 1077) is another “in-depth” sampling device which is designed to collect large samples which can be used for a multitude of tests. Basically, this collection apparatus is a hollow cylinder with a spring loaded plunger attached to each end. The device is cocked above the surface of the water and lowered to the desired depth. A weighted messenger is sent down the calibrated line to trip the closing mechanism and the plungers seal the sample from mixing with intermediate layers as it is brought to the surface. A special drain outlet is provided to draw oﬀ samples for chemical analysis.

Sampling of Closed System

To obtain representative samples from conﬁned water systems, such as pipe lines, tanks, vats, ﬁlters, water softeners, evaporators and condensers, diﬀerent considerations are required because of chemical changes which occur between the inlet and outlet water. One must have a basic understanding of the type of chemical changes which occur for the type of equipment used. Also, consideration should be given to the rate of passage and retaining time for the process water.

Temperature changes play an important part in deciding exactly what test should be performed. Process water should be allowed to come to room temperature, 20–25°C, before conducting any tests.

When drawing oﬀ samples from an outlet pipe such as a tap, allow sample to run for several minutes, rinsing the container several times before taking the ﬁnal sample. Avoid splashing and introduction of any contaminating material.

 FILTRATION

When testing natural waters that contain signiﬁcant turbidity due to suspended solids and algae, ﬁltration is an option. Reagent systems, whether EPA, Standard Methods, LaMotte or any others, will generally only determine dissolved constituents. Both EPA and Standard Methods suggest ﬁltration through a 0.45 micron ﬁlter membrane, to remove turbidity, for the determination of dissolved constituents.** To test for total constituents, organically bound and suspended or colloidal materials, a rigorous high temperature acid digestion is necessary.

**LaMotte oﬀers a ﬁltering apparatus: syringe assembly (Code 1050) and membrane ﬁlters, 0.45 micron, (Code 1103).

 AN INTRODUCTION TO COLORIMETRIC ANALYSIS

Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must ﬁnd a way to “see” them. The Smart3 Colorimeter can be used to measure any test substance that is itself colored or can be reacted to produce a color. In fact a simple deﬁnition of colorimetry is “the measurement of color” and a colorimetric method is “any technique used to evaluate an unknown color in reference to known colors”. In a colorimetric chemical test the intensity of the color from the reaction must be proportional to the concentration of the substance being tested. Some reactions have limitations or variances inherent to them that may give misleading results. Many such interferences are discussed with each particular test instruction. In the most basic colorimetric method the reacted test sample is visually compared to a known color standard. However, accurate and reproducible results are limited by the eyesight of the analyst, inconsistencies in the light sources, and the fading of color standards.

SMART3 Colorimeter 09.16 11

To avoid these sources of error, a colorimeter can be used to photoelectrically measure the amount of colored light absorbed by a colored sample in reference to a colorless sample (blank).

White light is made up of many diﬀerent colors or wavelengths of light. A colored sample typically absorbs only one color or one band of wavelengths from the white light. Only a small diﬀerence would be measured between white light before it passes through a colored sample versus after it passes through a colored sample. The reason for this is that the one color absorbed by the sample is only a small portion of the total amount of light passing through the sample. However, if we could select only that one color or band of wavelengths of light to which the test sample is most sensitive, we would see a large diﬀerence between the light before it passes through the sample and after it passes through the sample.

The Smart3 Colorimeter passes one of four colored light beams through one of four optical ﬁlters which transmits only one particular color or band of wavelengths of light to the photodectector where it is measured. The diﬀerence in the amount of colored light transmitted by a colored sample is a measurement of the amount of colored light absorbed by the sample. In most colorimetric tests the amount of colored light absorbed is directly proportional to the concentration of the test factor producing the color and the path length through the sample. However, for some tests the amount of colored light absorbed is inversely proportional to the concentration.

The choice of the correct wavelength for testing is important. It is interesting to note that the wavelength that gives the most sensitivity (lower detection limit) for a test factor is the complementary color of the test sample. For example the Nitrate-Nitrogen test produces a pink color proportional to the nitrate-nitrogen concentration in the sample (the greater the nitrate-nitrogen concentration, the darker the pink color). A wavelength in the green region should be selected to analyze this sample since a pinkish-red solution absorbs mostly green light.

 REAGENT BLANK

Some tests will provide greater accuracy if a reagent blank is determined to compensate for any color or turbidity resulting from the reagents themselves. A reagent blank is performed by running the test procedure on demineralized or deionized water. Use sample water to Scan Blank. Insert the reacted reagent blank in the colorimeter chamber and select Scan Sample. Note result of reagent blank. Perform the tests on the sample water as described. Subtract results of reagent blank from all subsequent test results. NOTE: Some tests require a reagent blank to be used to Scan Blank.

 COLORIMETER TUBES AND CHAMBER

The colorimeter uses one type of tube (Code 0290) for all test factors. The handling of the tubes is of utmost importance. Tubes must be clean and free from lint, ﬁngerprints, dried spills and signiﬁcant scratches, especially the central zone between the bottom and the sample line.

Scratches, ﬁngerprints and water droplets on the tube can cause stray light interference leading to inaccurate results. Tubes that have been scratched in the light zone through excessive use should be discarded and replaced with new ones.

Tubes should always be washed on the inside and outside with mild detergent prior to use to remove dirt or ﬁngerprints. The tubes should be allowed to air-dry in an inverted position to prevent dust from entering the tubes. Dry tubes should be stored with the caps on to prevent contamination.

12 SMART3 Colorimeter 09.16

After a tube has been ﬁlled and capped, it should be held by the cap and the outside surface should be wiped with a clean, lint-free absorbent cloth until it is dry and smudgefree. Handling the tube only by the cap will avoid problems from ﬁngerprints. Always set the clean tube aside on a clean surface that will not contaminate the tube. It is imperative that the tubes and light chamber be clean and dry. The outside of the tubes should be dried with a clean, lint-free cloth or disposable wipe before they are placed in the meter chamber.

Tubes should be emptied and cleaned as soon as possible after reading a sample to prevent deposition of particulates on the inside of the tubes.

Variability in the geometry of the glassware and technique is the predominate cause of variability in results. Slight variations in wall thickness and the diameter of the tubes may lead to slight variations in the test results. To eliminate this error the tubes should be placed in the chamber with the same orientation each time.

Chambers which have been scratched through excessive use should be discarded and replaced with a new one.

 METER CARE

The optical system of the Smart3 must be kept clean and dry for optimal performance. Dry the colorimeter tubes before placing them in the chamber to avoid introducing moisture. For best results store the instrument in a area that is dry and free from aggressive chemical vapors.

 SELECTING AN APPROPRIATE WAVELENGTH

The most appropriate wavelength to use when creating a calibration curve is usually the one which gives the greatest change from the lowest reacted standard concentration to the highest reacted standard concentration. However, the absorbance of the highest reacted standard concentration should never be greater than 2.0 absorbance units. Scan the lowest and highest reacted standards at diﬀerent wavelengths using the absorbance mode to ﬁnd the wavelength which gives the greatest change in absorbance without exceeding 2.0 absorbance units. Use this wavelength to create a calibration curve.

Below is a list of suggested wavelengths for the color of the reacted samples. Use these as a starting point.

Sample Color Wavelength Range

Yellow 428

Pink 525

Red 568

Green and Blue 635

 CALIBRATION

As with all pre-calibrated meters, it is highly recommended, even if not required by regulations, that the user periodically verify the performance of the meter by running standards with a predetermined concentration. Results outside of speciﬁcation are an indication that the meter needs to be adjusted. This can be done following the user calibration described on page 29. If the user calibration fails to properly adjust the meter then the meter should be returned to LaMotte Company for recalibration. (See page 74).

SMART3 Colorimeter 09.16 13

 CALIBRATION CURVES

The Smart3 Colorimeter contains tests for the LaMotte reagent systems. The ﬁrst step in using a non-LaMotte reagent system with your Smart3 Colorimeter is to create a calibration curve for the reagent system. To create a calibration curve, prepare standard solutions of the test factor and use the reagent system to test the standard solutions with the Smart3 Colorimeter. Select a wavelength for the test as described above.

Plot the results (in ABS or %Transmittance) versus concentration to create a calibration curve. The calibration curve may then be used to identify the concentration of an unknown sample by testing the unknown, reading Absorbance or %T, and ﬁnding the corresponding concentration from the curve. The linear range of the reagent system can be determined and this information can be used to input a User Test into the Smart3 Colorimeter (see Edit User Tests, page 42)

PROCEDURE

Prepare 5 or 6 standard solutions of the factor being tested. The concentration of these standards should be evenly distributed throughout the range of the reagent system, and should include a 0 ppm standard (distilled water). For instance, the solutions could measure 0, 10%, 30%, 50%, 70%, and 90% of the system’s maximum range.

1. Turn on the Smart3 Colorimeter. Select the appropriate wavelength from the absorbance mode. Be sure to select the appropriate wavelength for the color produced by the reagent system.

2. Use the unreacted 0 ppm standard to standardize the colorimeter by using it to scan blank.

3. Following the individual reagent system instructions, react each standard solution beginning with 0 ppm. Continue with standards in increasing concentration. Record the reading and the standard solution concentration on a chart. Readings can be recorded as percent transmittance (%T) or absorbance (A).

4. Plot results on graph paper or computer using any available plotting program. If results are as %T versus concentration, semilog graph paper must be used. Plot the standard solution concentrations on the horizontal, linear axis, and the %T on the vertical, logarithmic axis. If results are as absorbance versus standard solution concentration, simple linear graph paper can be used. Plot the standard solution concentration on the horizontal axis, and the absorbance on the vertical axis.

5. After plotting the results, draw a line, or curve, of best ﬁt through the plotted points. The best ﬁt may not connect the points. There should be approximately an equal number of points above the curve as below the curve. Some reagent systems will produce a straight line, while others produce a curve. Many computer spreadsheet programs can produce the curve of best ﬁt by regression analysis of the standard solution data.

NOTE: Only reagent systems which produce a straight line can be used for a User Test.

14 SMART3 Colorimeter 09.16

A sample of each type of graph appears below:

PREPARING DILUTE STANDARD SOLUTIONS

Standard solutions should be prepared to create a calibration curve. Standard solutions can be prepared by diluting a known concentrated standard by speciﬁed amounts. A chart or computer spreadsheet can be created to determine the proper dilutions. Use volumetric ﬂasks and volumetric pipets for all dilutions.

6. In Column A – Record the maximum concentration of test as determined by the range and path length.

7. In Column B – Record the percent of the maximum concentration the standard solution will be.

8. In Column C – Calculate the ﬁnal concentration of the diluted standard solutions by multiplying the maximum concentration (In Column A) by the % of maximum concentration divided by 100. (C = A x B/100).

9. In Column D – Record the ﬁnal volume of the diluted sample (i.e. volume of volumetric ﬂask).

10. In Column E – Record the concentration of the original standard.

11. In Column F – Calculate the milliliters of original standard required (F = (C x D/E)).

A sample chart appears below:

A B C =

Maximum

concentration

of test

% of Maximum

concentration

A x B/

100

Final

concentration

of Diluted Standard

D E F =

Volume of

Standard

Concentration

of Original

Standard

C x D/E

mL of

Original Standard Required

10.0 ppm 90 9.0 ppm 100 mL 1000 ppm 0.90 mL

10.0 ppm 70 7.0 ppm 100 mL 1000 ppm 0.70 mL

10.0 ppm 50 5.0 ppm 100 mL 1000 ppm 0.50 mL

10.0 ppm 30 3.0 ppm 100 mL 1000 ppm 0.30 mL

10.0 ppm 10 1.0 ppm 100 mL 1000 ppm 0.10 mL

10.0 ppm 0 0 ppm 100 mL 1000 ppm 0 mL

SMART3 Colorimeter 09.16 15

 STANDARD ADDITIONS

A common method to check the accuracy and precision of a test is by standard additions. In this method a sample is tested to determine the concentration of the test substance. A second sample is then “spiked” by the addition of a known quantity of the test substance. The second sample is then tested. The determined concentration of the spiked sample should equal the concentration of the ﬁrst plus the amount added with the spike. The procedure can be repeated with larger and larger “spikes.” If the determined concentrations do not equal the concentration of the sample plus that added with the “spike”, then an interference may exist.

For example, a 10.0 mL water sample was determined to contain 0.3 ppm iron. To a second

10.0 mL sample, 0.1 mL of 50 ppm iron standard was added. The concentration of iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50 ppm. The concentration of iron determined in the spiked sample should be 0.3 + 0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is negligible).

LaMotte oﬀers a line of calibration standards which can be used to generate calibration curves and perform standard additions.

 SAMPLE DILUTION TECHNIQUES & VOLUMETRIC MEASUREMENTS

If a test result using the Smart3 Colorimeter gives an over range message then the the sample must be diluted. The test should be repeated on the diluted sample to obtain a reading which is in the concentration range for the test. (Note: This is not true for colorimetric determination of pH.)

Example:

Measure 5 mL of the water sample into a graduated cylinder. Add demineralized water until the cylinder is ﬁlled to the 10 mL line. The sample has been diluted by one-half, and the dilution factor is therefore 2. Perform the test procedure, then multiply the resulting concentration by 2 to obtain the test result.

The following table gives quick reference guidelines on dilutions of various proportions. All dilutions are based on a 10 mL volume, so several dilutions will require small volumes of the water sample. Graduated pipets should be used for all dilutions.

Size of Sample Deionized Water to Bring Volume to 10 mL Multiplication Factor

10 mL 0 mL 1

5 mL 5 mL 2

2.5 mL 7.5 mL 4

1 mL 9 mL 10

0.5 mL 9.5 mL 20

If volumetric glassware is not available, dilutions can be made with the colorimeter tube. Fill the tube to the 10 mL line with the sample then transfer it to another container. Add 10 mL volumes of demineralized water to the container and mix. Transfer back 10 mL of the diluted sample to the tube and follow the test procedure. Continue diluting and testing until a reading, which is in the concentration range for the test, is obtained. Be sure to multiply the concentration found by the dilution factor (the number of total 10 mL volumes used).

16 SMART3 Colorimeter 09.16

Example:

10 mL of sample is diluted with three 10 mL volumes of demineralized water; the dilution factor is four.

 INTERFERENCES

LaMotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test. Be aware of possible interferences in the water being tested.

The reagent systems also contain buﬀers to adjust the water sample to the ideal pH for the reaction. It is possible that the buﬀer capacity of the water sample may exceed the buﬀer capacity of the reagent system and the ideal pH will not be obtained. If this is suspected, measure the pH of a reacted distilled water reagent blank using a pH meter. This is the ideal pH for the test. Measure the pH of a reacted water sample using the pH meter. If the pH is signiﬁcantly diﬀerent from the ideal value, the pH of the sample should be adjusted before testing.

Interferences due to high concentration of the substance being tested, can be overcome by sample dilution (see page 18)

 STRAY LIGHT INTERFERENCE

When scanning samples in 16 mm tubes, such as COD, the sample chamber lid can not be closed. The COD adapter minimizes stray light. To further reduce stray light interference, do not scan sample in direct sunlight.

OPERATION OF THE SMART3 COLORIMETER

 OVERVIEW

The Smart3 is a portable, microprocessor controlled, direct reading colorimeter. It has a graphical liquid crystal display and 6 button keypad. These allow the user to select options from the menu driven software, to directly read test results or to review stored results of previous tests in the data logger. The menus can be displayed in seven diﬀerent languages.

The test library consists of over 80 LaMotte tests and 25 “User Tests”. The LaMotte tests are precalibrated for LaMotte reagent systems.The colorimeter displays the result of these tests directly in units of concentration. The 25 “User Tests” may be used to enter additional calibrations. All of these tests may be arranged in any of 3 sequences. These sequences can be modiﬁed a limitless number of times to meet changing testing needs.

The optics feature 4 diﬀerent colored LEDs. Each LED has a corresponding silicon photoiode with an integrated interference ﬁlter. The interference ﬁlters select a narrow band of light from the corresponding LED for the colorimetric measurements. The microporcessor automatically selects the correct LED/photodiode combination for the test.

A USB wall adapter, USB computer connection or lithium battery powers the Smart3. Bluetooth wireless technology allows communication between the colorimeter and the

Bluetooth Mobile Printer (Code 3-0066 only).

SMART3 Colorimeter 09.16 17

 COMPONENTS

Figure 1 shows a diagram of the Smart3 Colorimeter and its components.

Top View

SMART3

ENTER

Bottom View

Serial Number

COLORIMETER

EXIT

Lid

Side Views

USB Port with plug

Figure 1

18 SMART3 Colorimeter 09.16

GENERAL OPERATING PROCEDURES

The operation of the Smart3 Colorimeter is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the colorimeter to perform, such as, scan blank, scan sample, and edit test sequences. The keypad is used to make menu selections which are viewed in the display. There are two selections accessible from the Main Menu: Testing Menu and Editing Menu.

 THE KEYPAD

The keypad has 6 buttons which are used to perform speciﬁc tasks.

This button will scroll up through a list of menu selections or go to the next character on a character selection line.

ENTER

EXIT

The button is used to select choices in a menu viewed in the display and to add the selected character to the name and advance one character.

This button controls the backlight on the display.

This button will scroll down through a list of menu selections or go to the previous character selection line.

This button exits to the previous menu on the name edit screen, the name will not be saved.

This button turns the meter on or oﬀ.

ENTER

EXI T

 SAMPLE HOLDERS

The sample chamber is designed for 25 mm round tubes. An adapter to hold 16 mm COD tubes and 10 mm square UDV cuvettes is included. 10 cm cuvettes can be scanned only at 525 nm and 568 nm. The light path is blocked at 428 nm and 635 nm with the use of the adapter. COD tubes can be scanned at all wavelengths.

SMART3 Colorimeter 09.16 19

Position the COD/UDV Adapter (Code 1724) so that the notches in the adapter ﬁt around the posts on the chamber. Turn the adapter counterclockwise until the arrows are at the top and bottom of the chamber and the adapter is locked into place. Turn the adapter clockwise to unlock the adapter and remove it from the chamber.

 THE DISPLAY & THE MENUS

The display allows menu selections to be viewed and selected. These selections instruct the Smart3 to perform speciﬁc tasks. The menus are viewed in the display using two general formats that are followed from one menu to the next. Each menu is a list of choices or selections.

The display has a header line at the top and a footer line at the bottom. The header displays the title of the current menu. The footer line displays the time and the date, the data logger status, the bluetooth/printer status and the battery status. The menu selection window is in the middle of the display between the header and the footer.

The menu selection window displays information in two general formats. In the ﬁrst format only menu selections are displayed. Up to 4 lines of menu selections may be displayed. If more selections are available they can be viewed by pressing the arrow buttons

to scroll the other menu selections into the menu selection window. Think of the menu selections as a vertical list in the display that moves up or down each time an arrow button is pressed. Some menus in the Smart3 are looping menus. The top and bottom menu choices are connected in a loop. Scrolling down past the bottom of the menu will lead to the top of the menu. Scrolling up past the top of the menu will lead to the bottom of the menu.

Header Menu Title

Main Window Selection First Choice

Second Choice

Third Choice

Another

Footer

12:00:00 001/500 P

And Another

And So On

A light bar will indicate the menu choice. As the menu is scrolled through, the light bar will highlight diﬀerent menu choices. Pressing the

ENTER

button will select the menu choice

that is indicated by the light bar. In the second format the menu choice window takes advantage of the graphical

capabilities of the display. Large format graphic information, such as test results or error messages or the LaMotte logo is displayed. The top two lines of the display are used to display information in a large, easy to read format. The menus work in the same way as previously described but two lines of the menu are visible at the bottom of the display.

20 SMART3 Colorimeter 09.16

Header Menu Title

Message or Result Window

Result or Message

Another

Main Window Selection And Another

Footer

12:00:00 001/500

And So On

Last Choice

As described previously, the and a return to the previous menu. This allows a rapid exit from an inner menu to the main menu by repeatedly pushing the Smart3 oﬀ.

The display may show the following messages:

Battery Status

More choices are available and can be viewed by scrolling up and/or down through the display.

Header Identiﬁes the current menu and information on units and reagent

systems if applicable.

Footer In the data logging mode the number of the data point is displayed

and the total number of data points in the memory will be shown. The footer also shows current time and battery status

EXIT

button allows an exit or escape from the current menu

EXIT

button. Pushing at any time will turn the

SMART3 Colorimeter 09.16 21

TESTING

 TESTING MENU

The Testing Menu is used to run all LaMotte pre-programmed tests, User Tests and Absorbance tests at one of four wavelengths. Testing from any of three sequences can also be done.

1. Press and brieﬂy hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

ENTER

to select Testing Menu.

3. Press or to scroll to desired option. All Tests contains all of the available pre-programmed tests. The three sequences have user selected tests. Absorbance has %T/ABS tests.

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

Testing Menu

All Tests Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

Testing Menu

All Tests Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

4. Press

ENTER

to select All Tests.

All Tests

002 Alkalinity UDV

005 Aluminum

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

22 SMART3 Colorimeter 09.16

TEST SEQUENCES

Sequence 1, Sequence 2, And Sequence 3 are alterable sequences. They may be edited using the Editing Menu. Any of the LaMotte pre-programmed tests or User Tests may be placed in these sequences in whatever testing order that is preferred. There are three intial sequences in the Smart3 that can be customized by adding or deleting tests:

Sequence 1 Sequence 2 Sequence 3

025 Chlorine T DPD 005 Aluminum 006 Ammonia-N LF

080 Phosphate HR 025 Chlorine T DPD 036 Cu Thiocarbamate

010 Benzotriazole

056 Iron Phenanthro 065 Nitrate-N LR

075 pH UDV 060 Manganese LR 068 Nitrite-N LR

12:00:00 001/500 12:00:00 001/500 12:00:00 001/500

063 Molybdenum HR 065 Nitrate-N LR 075 pH PR

086 Silica HR 068 Nitrite-N LR 081 Phosphate LR

036 Cu Thicarbamate 079 Phenol 087 Silica LR

055 Iron Bipyridyl 081 Phosphate LR

090 Sulﬁde LR

These alterable sequences allow a series of tests to be setup that are run frequently. The order of the individual tests in the sequence can be determined by the user. After running a test, press

ENTER

to select the next test in the sequence. Continue this pattern until the

entire sequence has been completed. All Tests is a ﬁxed sequence containing the LaMotte pre-programmed tests, User Tests,

and Absorbance tests. Modiﬁcation of the alterable sequences is accomplished through the Editing Menu. This menu is explained in greater detail in Editing Menu (page 36).

Pressing

EXIT

while in a sequence menu will escape back to the Testing Menu. Pressing the at any time will turn the colorimeter oﬀ. NOTE: A sequence must contain at least one test.

 GENERAL TESTING PROCEDURES

The following is a step-by-step example of how to run tests from the Testing Menu. These test procedures are designed to be used with LaMotte SMART Reagent Systems.

LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available. Call LaMotte Technical Services at 1-800-344-3100 (410778-3100 outside the USA) or email at tech@lamotte.com for a current list of available calibrations.

SMART3 Colorimeter 09.16 23

 TESTING WITH LaMOTTE PRE-PROGRAMMED TESTS

1. Press and brieﬂy hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

3. Press

ENTER

to select Testing Menu.

ENTER

to select All Tests Menu.

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

Testing Menu

All Test Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

All Tests

002 Alkalinity UDV

005 Aluminum

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

4. Press or to scroll to the desired test.

002 Alkalinity UDV

All Tests

005 Aluminum

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

5. Press

ENTER

to select the test.

005 Aluminum

Scan Bank

Scan Sample

12:00:00 001/500

24 SMART3 Colorimeter 09.16

6. Insert the blank into the chamber. Close the lid.Press screen wil display Scan Blank Blank Done for about 1 second and then return to the test menu.

ENTER

to scan the blank. The

005 Aluminum

Scan Blank

Scan Sample

12:00:00 001/500

7. Insert the reacted sample into the chamber. Close the lid. Press scan the sample. The screen will display Scan Sample Sample Done for about 1 second. The result will appear on the screen.

To repeat the test, press sample again. The last blank scanned is used by the colorimeter for repeated scans. A diﬀerent blank can be used by pressing or to scroll to Scan Blank and then scanning another blank. Scroll with or

and make another selection with . The %T or Absorbance of the last test can be viewed by scrolling down and choosing %T/ Abs. Press NOTE: The menus loop in this screen so either

needed.

To use the Print Test function, see the Bluetooth Menu section (page 60).

EXIT

to escape to previous menus.

or will lead to the menu selection

ENTER

to

to scan the

ENTER

1.00 ppm

Scan Blank

Scan Sample

12:00:00 001/500

1.00 ppm

Scan Bank

Scan Sample

12:00:00 001/500

005 Aluminum

 CALIBRATING LaMOTTE PRE-PROGRAMMED TESTS

The LaMotte Pre-Programmed Tests have been pre-calibrated. Recalibration of the preprogrammed tests by the user is not possible. However, a procedure to standardize the calibration can be performed to obtain the most accurate readings or to meet regulatory requirements.

The LaMotte Pre-Programmed tests are standardized with one standard solution. To standardize over the full range of the test, the concentration of the standard should be chosen from the high end of the range. Alternatively, if samples do not cover the full range of the test, a standard should be chosen that is close to the concentration of the samples.

The standardization procedure should be followed as often as required by regulations and laws for compliance monitoring.

In the example below, the Aluminum calibration will be standardized. Prepare a standard solution to be tested. In this example, 0.30 ppm aluminum.

SMART3 Colorimeter 09.16 25

1. Press and brieﬂy hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

2. Press

3. Press

ENTER

to select Testing Menu.

ENTER

to select All Tests Menu.

4. Press or to scroll to the desired test factor.

Testing Menu

All Test Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

All Tests

002 Alkalinity UDV

005 Aluminum

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

All Tests

002 Alkalinity UDV

005 Aluminum

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

5. Press

ENTER

to select the test.

005 Aluminum

Scan Blank

Scan Sample

12:00:00 001/500

26 SMART3 Colorimeter 09.16

6. Follow the test procedure in the manual to test the prepared standard. Insert the blank into the chamber. Close the lid. Press

ENTER

to scan the blank. The screen will display Scan Blank Blank Done for about 1 second and then return to the Test Menu.

005 Aluminum

Testing

Scan Blank

Scan Sample

12:00:00 001/500

7. Insert the reacted standard solution into the chamber. Close the lid. Press scan the sample. The screen will display Scan Sample Sample Done for about 1 second. The result will appear on the screen.

8. The displayed result can now be standardized. Press or to scroll to Calibrate.

9. Press

ENTER

to select Calibrate. A reverse font (light background with dark characters) will appear to indicate that the reading can be adjusted.

ENTER

005 Aluminum

to

0.28 ppm

Scan Blank

Scan Sample

12:00:00 001/500

005 Aluminum

0.28 ppm

%T/Abs

Calibrate

12:00:00 001/500

005 Aluminum

0.28 ppm

∧, ∨=Edit, ENTER=Save

∧ +ENTER=Default

12:00:00 001/500

10. Press or to adjust the value shown to the concentration of the prepared standard, 0.30 in this example.

NOTE: A maximum adjustment of 25% is possible.

SMART3 Colorimeter 09.16 27

0.30 ppm

∧, ∨=Edit, ENTER=Save

∧ +ENTER=Default

12:00:00 001/500

005 Aluminum

11. Press

ENTER

to save the value.

To leave the Calibration procedure without saving the adjustment, press

EXIT

.

Testing

Press and

ENTER

at any time to

return to the default value.

The calibration has now been standardized and can be used for testing. Scroll to Scan Blank and begin testing.

MEASURING IN THE ABSORBANCE MODE

005 Aluminum

0.30 ppm

%T/Abs

Calibrate

12:00:00 001/500

1. Press and brieﬂy hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

2. Press

ENTER

to select Testing Menu.

3. Press or to scroll to Absorbance.

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

Testing Menu

All Test Menu

Sequence 1

Sequence 2

Sequence 3

12:00:00 001/500

Testing Menu

Sequence 1

Sequence 2

Sequence 3

Absorbance

12:00:00 001/500

28 SMART3 Colorimeter 09.16

4. Press

ENTER

to select Absorbance.

Absorbance

101 Absorbance 428

102 Absorbance 525

103 Absorbance 568

104 Absorbance 635

12:00:00 001/500

Testing

5. Press or to scroll to desired wavelength.

6. Press

7. Insert the blank. Close the lid. Press

ENTER

to select the wavelength.

ENTER

to scan the blank. The screen wil display Scan Blank Blank Done for about 1 second and return to the Absorbance menu.

Absorbance

101 Absorbance 428

102 Absorbance 525

103 Absorbance 568

104 Absorbance 635

12:00:00 001/500

102 Absorbance 525

Scan Blank

Scan Sample

12:00:00 001/500

102 Absorbance 525

Scan Blank

Scan Sample

12:00:00 001/500

8. Insert the reacted sample. Press

ENTER

to

102 Absorbance 525

scan the sample. The screen will display Scan Sample Sample Done for about

0.425

1 second. The result will appear on the screen.

Scan Sample

Next Test

12:00:00 001/500

SMART3 Colorimeter 09.16 29

9. To repeat the test, scroll to Scan Sample and press again. The last blank scanned is used by the colorimeter for repeated scans. A diﬀerent blank can be used by pressing

Testing

and then scanning another blank. Scroll with or and make another selection with Absorbance of the last test can be viewed by choosing %T/Abs. Press escape to previous menus. NOTE: The menus loop in this screen so either or will lead to the menu selection needed. NOTE: The calibrate function does not work in the Absorbance mode.

ENTER

to scan the sample

or to scroll to Scan Blank

ENTER

. The %T or

EXIT

to

0.425

Scan Sample

Next Test

12:00:00 001/500

Previous Test

%T/Abs

Calibrate

Print Test

102 Absorbance 525

EDITING MENU

The Editing Menu allows the user to edit sequences, edit user tests, set the clock, edit the logging function, access factory setting, set the power saving function, set the backlight time, and select a language.

The default factory settings are:

Date Format MM-DD-YYYY

Logging Enabled

Power Save 5 minutes

Backlight 10 seconds

Language English

 EDITING A SEQUENCE

The Edit Sequence menu allows three alterable test sequences (Sequence 1, Sequence 2, Sequence 3) to be edited.

1. Press and brieﬂy hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

30 SMART3 Colorimeter 09.16

Testing Menu

Editing Menu

12:00:00 001/500

Main Menu

2. Press or to scroll to the

Editing Menu.

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

Testing

3. Press

4. Press

ENTER

to select Editing Menu.

ENTER

to select Edit Sequences.

5. Press or to scroll to the desired sequence.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Edit Sequences

Edit Sequence 1

Edit Sequence 2

Edit Sequence 3

12:00:00 001/500

Edit Sequences

Edit Sequence 1

Edit Sequence 2

Edit Sequence 3

12:00:00 001/500

6. Press edited.

ENTER

to select the sequence to be

005 Aluminum

EDIT SEQUENCE 2

025 Chlorine T DPD

056 Iron Phenanthro

060 Manganese LR

12:00:00 001/500

SMART3 Colorimeter 09.16 31

ADDING OR DELETING A TEST

There are three ways to alter a sequence: Insert Before, Insert After, and Delete. Insert Before adds a new test to the sequence before the selected test. Insert After adds a new test to the sequence after the selected test. Delete is used to remove an existing test from a sequence.

Testing

ADDING A TEST

Below is a step-by-step example of how to add a test to SEQUENCE 2 starting from the EDIT SEQUENCE 2 menu.

1. To add a test before or after an existing test, press or to scroll to the existing test.

2. Press

ENTER

to select the existing test.

3. Press or to scroll to Insert Before or Insert After.

EDIT SEQUENCE 2

005 Aluminum

025 Chlorine T DPD

056 Iron Phenanthro

060 Manganese LR

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

4. Press

ENTER

to select the option, Insert

Before, in this example. The All Test Menu

will appear.

002 Alkalinity UDV

005 Aluminum

All Tests

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

32 SMART3 Colorimeter 09.16

5. Press or to scroll to the test that will be added to the sequence. In this example, 006 Ammonia-N LRS.

All Tests

002 Alkalinity UDV

005 Aluminum

006 Ammonia-N LRF

007 Ammonia-N LRS

12:00:00 001/500

Testing

6. Press

ENTER

to select the test. The sequence will appear in the Edit Sequence menu and the new test will be added to the sequence. All changes in the sequence will be automatically saved.

7. Press

EXIT

to exit the Edit Sequence menu and return to the Editing Sequences Menu.

8. Press

ENTER

to select Edit Sequences to continue editing the sequences or

EXIT

press

twice to return to the Main

Menu.

EDIT SEQUENCE 2

005 Aluminum

025 Chlorine T DPD

006 Ammonia-N LF

056 Iron Phenanthro

12:00:00 001/500

Edit Sequences

Edit Sequence 1

Edit Sequence 2

Edit Sequence 3

12:00:00 001/500

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

SMART3 Colorimeter 09.16 33

DELETING A TEST

Below is a step-by-step example of how to delete a test in SEQUENCE 2 starting from the EDIT SEQUENCE 2 menu.

1. To delete a test, press or to scroll to the test in the sequence.

Testing

2. Press

ENTER

to select the test.

3. Press or to scroll to Delete.

EDIT SEQUENCE 2

005 Aluminum

025 Chlorine T DPD

006 Ammonia-N LF

056 Iron Phenathro

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

Add or Delete

Insert Before

Insert After

Delete

12:00:00 001/500

4. Press

ENTER

to select Delete. The sequence will appear in the Edit Sequences menu and the selected test will have been deleted. All changes to the sequence will automatically have been saved.

5. Press

EXIT

to exit the Edit Sequence menu and return to the Edit Sequences Menu.

005 Aluminum

025 Chlorine T DPD

056 Iron Phenanthro

060 Manganese LR

12:00:00 001/500

Edit Sequence 1

Edit Sequences 2

EDIT SEQUENCE 2

Edit Sequences

Edit Sequences 3

12:00:00 001/500

34 SMART3 Colorimeter 09.16

6. Press

EXIT

to exit the Edit Sequences

menu and return to the Editing Menu.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Testing

7. Press

ENTER

to select Edit Sequences to

continue editing the sequences or press

EXIT

to return to the Main Menu.

Testing Menu

Editing Menu

12:00:00 001/500

Main Menu

 EDIT USER TESTS

If a test other than the LaMotte programmed tests is performed regularly, a calibration for it may be entered in one of the 25 User Tests. These tests are originally named “User Test 1 - 25”. It will be possible to rename the test, select a wavelength, enter a new calibration, select the number of decimal places used to display the results, and select the units. A User Test may be added for a reagent system for which no precalibrated test exists. A calibration of a LaMotte reagent system may also be entered. The calibration of a User Test can be changed at any time.

The User Tests have the ability to handle 2 data points. The colorimeter will determine the absorbance of the standards and calculate a response that will be stored to determine the concentration of future samples of unknown concentration. These standards should cover all the concentrations for the range of the test being performed and be scanned beginning with the low concentration and ﬁnishing with the high concentration (for more information about this, see CALIBRATION CURVES, page 16). Prepare these standards prior to entering a new calibration.

NOTE: A calibration procedure must be performed before using any of the User Tests. The User Tests can be placed in any of the alterable sequences using Edit Sequences.

1. Press and brieﬂy hold to turn the meter on. The LaMotte logo screen will appear for about 3 seconds and the Main Menu will appear.

SMART3 Colorimeter 09.16 35

Testing Menu

Editing Menu

12:00:00 001/500

Main Menu

2. Press or to scroll to the

Editing Menu.

Testing

Main Menu

Testing Menu

Editing Menu

12:00:00 001/500

3. Press

ENTER

to select Editing Menu. Press

to scroll to Edit User Test.

4. Press

ENTER

to select Edit User Test.

5. Press or to scroll to the desired user test.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Edit User Test

105 USER TEST 01

106 USER TEST 02

107 USER TEST 03

108 USER TEST 04

12:00:00 001/500

Edit User Test

108 USER TEST 04

109 USER TEST 05

110 USER TEST 06

111 USER TEST 07

12:00:00 001/500

6. Press

ENTER

to select the User Test.

111 USER TEST 07

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

36 SMART3 Colorimeter 09.16

 NAMING THE TEST

A User Test can be up to 16 characters long. The menu choices for each character are 26 upper case letters A to Z, 26 lower case letters a to z, ten numerals 0 to 9, a space, a dash (-) and a decimal point (.). The existing name is displayed on the bottom line of the display. The character which is to be edited will blink and that character is also displayed in the center of the display. The character can be changed by using or to scroll to other characters. Use pressing

EXIT

ENTER

to select a character. The edited name is saved at any time by

or by pressing

ENTER

after selecting the sixteenth character.

Testing

1. From the User Test menu, press to scroll to Name the Test.

2. Press

ENTER

to select Name the Test. A blinking reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will also appear in the center of the display.

3. Press or to scroll to the desired character. In this example, the test name will be H2O.

The character for a blank space is located after the lower case z.

111 USER TEST 07

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

Name the Test

P Q R S T U V W X Z

111 U SER TEST 07

12:00:00 001/500

Name the Test

C D E F G H I J K L M

111 U SER TEST 07

12:00:00 001/500

4. Press

ENTER

to save the character and

Name the Test

move to the next character.

N O P Q R S T U V W X

111 H S ER TEST 07

12:00:00 001/500

SMART3 Colorimeter 09.16 37

5. Press or to scroll to the desired character.

Name the Test

X Y Z . 0 1 2 3 4 5 6 7

111 H S ER TEST 07

12:00:00 001/500

6. Press

ENTER

to save the character and

move to the next character.

7. Press or to scroll to the

Editing/Set Up

desired character.

8. Press

ENTER

to save the character. Repeat the procedure until the test name is complete.

After selecting the last character in the name: Hold down and press and release

ENTER

to save all selected characters and to delete all characters to the right of the cursor and exit the name edit screen. Hold down and press and release

ENTER

to save all remaining characters and exit the name edit screen. The meter will return to the Edit Test manu.

Name the Test

9 A B C D E F G H I J K

111 H2 E R TEST 07

12:00:00 001/500

Name the Test

J K L M N O P Q R S T

111 H2 O R TEST 07

12:00:00 001/500

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

38 SMART3 Colorimeter 09.16

 SELECT THE VIAL AND WAVELENGTH

The Smart3 Colorimeter accepts three diﬀerent vials (the 25 mm 0290 tube, UDVs and COD tubes) at 4 diﬀerent wavelengths (428, 525, 560, and 635 nm). The colorimeter uses diﬀerent settings for each of the twelve combinations of vial and wavelength.

9. From the User Test menu, press or to scroll to Select Vial/WL.

10. Press

ENTER

to select Select Vial/WL.

11. Press or to scroll to the

channel with the desired wavelingth and vial size combination. The screen will display storing... for about 1 second and the meter will return to the User Test menu.

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

Select Vial/WL

Ch1 428nm 25mm

Ch2 525nm 25mm

Ch3 635nm 25mm

Ch4 568nm 25mm

12:00:00 001/500

Select Vial/WL

Ch1 428nm 25mm

Ch2 525nm 25mm

Ch3 635nm 25mm

Ch4 568nm 25mm

12:00:00 001/500

Editing/Set Up

12. Press

ENTER

to select the Vial/WL option. The screen will display Storing... for about 1 second and the meter will return to the Edit Test menu.

Name the Test

Select Vial/WL

111 H2O

STD Calibration

Enter Constants

12:00:00 001/500

 ENTERING A TWO POINT CALIBRATION

The Smart3 Colorimeter can scan two reacted standards and create a calibration curve. To create a calibration curve with multiple data points see Entering a Multiple Point Calibration Curve (pg. 51).

SMART3 Colorimeter 09.16 39

1. From the User Test menu, press or to scroll to STD (Standard) Calibration.

111 H2O

Name the Test

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

2. Press

ENTER

to select STD Calibration. The values for the standards will be entered. The screen will display the Low Standard screen. A blinking reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will also appear in the center of the display.

Editing/Set Up

3. Press or to scroll to the ﬁrst character of the low concentration. In this example, 1.00 ppm.

4. Press

ENTER

to save the character and

move to the next character.

Low Standard

6 7 8 9 . - 0 1 2 3 4 5

0 0.000000

12:00:00 001/500

Low Standard

7 8 9 . - 0 1 2 3 4 5 6

0 .000000

12:00:00 001/500

Low Standard

5 6 7 8 9 . - 0 1 2 3 4

1 . 000000

12:00:00 001/500

5. Press or to scroll to the

Low Standard

desired character.

5 6 7 8 9 . - 0 1 2 3 4

1 . 000000

12:00:00 001/500

40 SMART3 Colorimeter 09.16

6. Press

ENTER

to save the character and

move to the next character.

Low Standard

6 7 8 9 . - 0 1 2 3 4 5

1. 0 00000

12:00:00 001/500

7. Press or to scroll to the desired character.

8. Press

ENTER

to save the character. Repeat the procedure until the low concentration value is complete. (the character for a blank space is located after the 9.) After the ﬁnal character is complete the meter will save the low concentration value. The screen will display the High Standard screen. A blinking reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will appear in the center of the display.

9. Use or and

ENTER

to select the characters for the high concentration value. In this example, 7.5 ppm.

Low Standard

6 7 8 9 . - 0 1 2 3 4 5

1. 0 00000

Editing/Set Up

12:00:00 001/500

High Standard

6 7 8 9 . - 0 1 2 3 4 5

0 .0000

12:00:00 001/500

High Standard

6 7 8 9 . - 0 1 2 3 4 5

7.50000 0

12:00:00 001/500

SMART3 Colorimeter 09.16 41

10. After the ﬁnal character is entered the meter will save the high concentration value. The screen will display instructions for completing the calibration procedure.

STD Calibration

Insert Blank

<Enter> continue

12:00:00 001/500

11. The standards wil be scanned. Insert the blank. Press

ENTER

and the Insert Low

Standard screen will appear.

12. Insert the low standard. Press

Editing/Set Up

ENTER

The Insert High Standard screen will be displayed.

13. Insert the high standard. Press

ENTER

meter will display the constants for the calibration curve.

.

. The

STD Calibration

Insert Low Standard

<Enter> continue

12:00:00 001/500

STD Calibration

Insert High Standard

<Enter> continue

12:00:00 001/500

K0 = 0.57246

K1 = 7.14547

K2 = 0.00000

K3 = 0.00000

<Enter> Save

12:00:00 001/500

14. Press

ENTER

to save the calibration curve

and return to the Edit User Test Menu.

Name the Test

111 H2O

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

 ENTERING A MULTIPLE POINT CALIBRATION

The Smart3 can directly create a 2 point calibration curve. (See Entering a Two Point Calibration on page 48.) To create a multiple point calibration curve, constants obtained

42 SMART3 Colorimeter 09.16

from a linear regression of multiple data points can be entered into the Smart3.

1. Scan reactions of multiple concentrations at the appropriate wavelength in the absorbance mode on the Smart3.

2. Plot the concentration (y axis) versus absorbance (x axis) in a program capable of linear regression such as Excel.

3. Enter the constants obtained from the linear regression equation into the Smart3.

For Example: y = 0.001x3 – 0.017x2 + 0.181x – 0.049 K0 = – 0.049 K1 = 0.181 K2 = – 0.017 K3 = 0.001 OR (Over Range) = 10

1. From the User Test menu, press or to scroll to Enter Constants.

Name the Test

111 H2O

Select Vial/WL

STD Calibration

Enter Constants

12:00:00 001/500

Editing/Set Up

2. Press

ENTER

to select Enter Constants.

K0=0.00000

K1=1.00000

K2=0.00000

K3=0.00000

OR=100.00000

12:00:00 001/500

3. Press

ENTER

to begin entering the values

Enter Constants K0

for the constants. A reverse font (dark background with a light character) will appear to indicate the character that will be adjusted. The same character will also

6 7 8 9 . - 0 1 2 3 4 5

0 .00000

appear in the center of the display.

12:00:00 001/500

SMART3 Colorimeter 09.16 43

4. Press or to scroll to the ﬁrst character of K0. In this example, –

0.049.

Enter Constants K0

6 7 8 9 . - 0 1 2 3 4 5

0 .00000

12:00:00 001/500

5. Press

ENTER

to save the character and

move to the next character.

6. Press or to scroll to the next

Editing/Set Up

character.

7. Press

ENTER

to save the character and move to the next character. Press or to scroll to the next character.

Note: The character for a blank space is located after the 9.

Enter Constants K0

5 6 7 8 9 . - 0 1 2 3 4

– . 00000

12:00:00 001/500

Enter Constants K0

6 7 8 9 . - 0 1 2 3 4 5

– . 00000

12:00:00 001/500

Enter Constants K0

5 6 7 8 9 . - 0 1 2 3 4

– 0 0 0000

12:00:00 001/500

8. Press

ENTER

to save the character.

Enter Constants K1

Repeat the procedure until the K0 value is complete. After the ﬁnal character is complete the meter will save the K0 value and the meter will display K1 screen.

44 SMART3 Colorimeter 09.16

6 7 8 9 . - 0 1 2 3 4 5

0 .00000

12:00:00 001/500

9. Use , and

ENTER

to select the characters for the remaining values: K1, K2, K3, and over range. In this example, 10 ppm.

Constants OR

6 7 8 9 . - 0 1 2 3 4 5

10.00000 0

12:00:00 001/500

10. After the ﬁnal character is entered the meter will save the constants. The screen will display Storing... and return to the Edit Test menu.

Name the Test

Select Vial/WL

111 H2O

STD Calibration

Enter Constants

12:00:00 001/500

 SELECTING THE NUMERICAL FORMAT OF THE RESULT

To input tests with very diﬀerent ranges, the number of decimal places displayed for a result can be selected. A test which ranges from 20 to 1000 ppm should not be displayed with three decimal places. A test with a range from 0.010 to 0.500 needs three decimal places (the microprocessor will always calculate the concentration to many more signiﬁcant ﬁgures than will be displayed). The choice of 0, 1, 2, or 3 decimal places are available.

1. From the User Test menu, press or

to scroll to Decimal Places.

Select Vial/WL

STD Calibration

Enter Constants

Decimal Places

12:00:00 001/500

111 H2O

Editing/Set Up

2. Press

ENTER

to select Decimal Places.

Decimal Places

None 0

One 0.0

Two 0.00

Three 0.000

12:00:00 001/500

SMART3 Colorimeter 09.16 45

3. Press or to scroll to the desired number of decimal places.

Decimal Places

None 0

One 0.0

Two 0.00

Three 0.000

12:00:00 001/500

4. Press

ENTER

to select the decimal places. The screen wil display Storing... for about 1 second and the meter will return to the Edit Test menu.

Select Vial/WL

STD Calibration

111 H2O

Enter Constants

Decimal Places

12:00:00 001/500

Editing/Set Up

 SELECTING THE UNITS OF CONCENTRATION

The Smart3 Colorimeter has seven options for units of concentration. They are No Units, ppm, pH, FAU, ppb, ppt and mgL.

1. From the User Menu, press to scroll to Select Units.

STD Calibration

111 H2O

Enter Constants

Decimal Places

Select Units

12:00:00 001/500

2. Press

ENTER

to select Select Units.

Select Units

No Units

ppm

pH

FAU

12:00:00 001/500

3. Press or to scroll to the desired units.

No Units

Select Units

ppm

pH

FAU

12:00:00 001/500

46 SMART3 Colorimeter 09.16

4. Press

ENTER

to select the units. The screen will display Storing... for about 1 second and the meter will return to the Edit Test menu.

STD Calibration

Enter Constants

111 H2O

Decimal Places

Select Units

12:00:00 001/500

 SETTING THE CLOCK

Setting the clock allows the correct time and date stamp to be stored with each reading in the data logger.

1. From the Editing Menu, press or to scroll to Set Clock.

2. Press

ENTER

to select Set Clock. The year is displayed. Press or to scroll to the appropriate character. Press

ENTER

to select the character. The month, day, hour, format hour, minute, second, AM/PM will be dislpayed. Repeat for each.

3. Press

ENTER

to select the ﬁnal character. The time and date will be saved and the meter will return to the Editing Menu.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Set Time

Year: 2000

12:00:00 001/500

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

Editing/Set Up

 LOGGING DATA

The default setting for the data logger is enabled. The meter will log the last 500 data points. The counter in the center bottom of the display will show how many data points have been logged. The display will show 500+ when the data logger has exceeded 500 points and the data points are being overwritten.

SMART3 Colorimeter 09.16 47

1. From the Editing Menu, press or to scroll to Logging.

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

2. Press

3. Press or to scroll to desired

Editing/Set Up

ENTER

to select Logging.

function.

4. Press

ENTER

. The screen will display Storing... for about 1 second and return to the Editing Menu.

Logging

Display Test Log

Logging Enabled

Logging Disabled

Erase Log

12:00:00 001/500

Logging

Display Test Log

Logging Enabled

Logging Disabled

Erase Log

12:00:00 001/500

Editing Menu

Edit Sequences

Edit User Test

Set Clock

Logging

12:00:00 001/500

 FACTORY SETUP

The Factory Setup menu is used in manufacturing of the Smart3 Colorimeter. This menu is not for use by the operator in the ﬁeld.

 SETTING POWER SAVE

The power saving Auto Shutoﬀ feature will turn the meter oﬀ when a button has not been pushed for a set amount of time. The default setting is disabled. To change the setting:

48 SMART3 Colorimeter 09.16

1. From the Editing Menu, press or to scroll to Set Power Save.

Editing Menu

Set Clock

Logging

Factory Setup

Set Power Save

12:00:00 001/500

2. Press

ENTER

to select Set PWR Save.

3. Press or to scroll to desired

function.

4. Press

ENTER

. The screen will display

Storing.... for about 1 second and the

meter will retun to the Editing Menu.

Set PWR Save

Disable

5 Minutes

15 Minutes

30 Minutes

12:00:00 001/500

Set PWR Save

Disable

5 Minutes

15 Minutes

30 Minutes

12:00:00 001/500

Editing Menu

Set Clock

Logging

Factory Setup

Set PWR Save

12:00:00 001/500

Editing/Set Up

 SETTING THE BACKLIGHT TIME

The backlight illuminates the display for enhanced viewing. The default setting is 10 seconds. If Button Control is chosen the backlight button on the key pad will act as an on/ oﬀ switch and the backlight will remain on or oﬀ when the meter is being used. When one of the other settings – 10, 20 or 30 seconds – is chosen, the display will be illuminated for the speciﬁed amount of time after any button is pressed. NOTE: The backlight feature uses a signiﬁcant amount of power. The longer the backlight is on, the more frequently the battery will have to be charged if the USB/Wall Adapter is not being used.

SMART3 Colorimeter 09.16 49

1. From the Editing Menu, press or to scroll to Backlight Time.

Editing Menu

Logging

Factory Setup

Set Power Save

Set Backlight Time

12:00:00 001/500

2. Press

ENTER

to select Set Backlight

Time.

3. Press or to scroll to desired

Editing/Set Up

option.

4. Press

ENTER

. The screen will display Storing... for about 1 second and the meter will return to the Editing Menu.

Set Backlight Time

Button Control

10 seconds

20 seconds

30 seconds

12:00:00 001/500

Set Backlight Time

Button Control

10 seconds

20 seconds

30 seconds

12:00:00 001/500

Editing Menu

Logging

Factory Setup

Set Power Save

Set Backlight Time

12:00:00 001/500

 BLUETOOTH MENU

Enabling Bluetooth Power

Bluetooth wireless technology allows communication between the colorimeter and a Bluetooth Mobile Printer (Code 5-0066 only). Bluetooth Power must be enabled to use the Bluetooth Mobile Printer (Code 5-0066). When Bluetooth Power is enabled, the Bluetooth icon will ﬂash next to the battery icon on the bottom line of the display.

If the Bluetooth feature is not being used to connect to the printer, Bluetooth Power should be disabled to conserve the battery life. The default Bluetooth Power setting is disabled.

50 SMART3 Colorimeter 09.16

1. From the Editing Menu, press or to scroll to Bluetooth Menu.

Editing Menu

Factory Setup

Set Power Save

Set Backlight Time

Bluetooth Menu

12:00:00 001/500

2. Press

ENTER

to select Bluetooth Menu.

3. Press or to scroll to

Bluetooth Power.

4. Press

ENTER

to select Bluetooth

Power.

Bluetooth Menu

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500

Bluetooth Menu

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500

Bluetooth Power

Enable Bluetooth

Disable Bluetooth

12:00:00 001/500

Editing/Set Up

5. Press or to scroll to desired

option.

Enable Bluetooth

Bluetooth Power

Disable Bluetooth

12:00:00 001/500

SMART3 Colorimeter 09.16 51

6. Press

ENTER

to select the option. Bluetooth Power must be enabled to use the Bluetooth Mobile Printer (Code 5-0066).

The screen will display Storing… for about

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

Bluetooth Menu

1 second and the meter will return to the Bluetooth Menu.

12:00:00 001/500 P

A ﬂashing P symbol will indicate that Bluetooth is enabled.

Setting The Bluetooth Mode

A Bluetooth connection is available only for the Bluetooth Mobile Printer (Code 5-0066).

1. From the Editing Menu, press or

Editing/Set Up

2. Press

to scroll to Bluetooth Menu.

ENTER

to select Bluetooth Menu.

3. Press or to scroll to Bluetooth Mode.

Editing Menu

Factory Setup

Set Power Save

Set Backlight Time

Bluetooth Menu

12:00:00 001/500 P

Bluetooth Menu

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

Bluetooth Menu

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

52 SMART3 Colorimeter 09.16

4. Press Mode.

ENTER

to select Bluetooth

Bluetooth Mode

Mobile Device

BT Printer

12:00:00 001/500 P

5. Press or to scroll to BT Printer.

Mobile Device

Bluetooth Mode

BT Printer

12:00:00 001/500 P

6. Press

ENTER

. The screen will display Storing… for about 1 second and the meter will return to the Bluetooth Menu.

Bluetooth Power

Bluetooth Mode

Bluetooth Menu

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

Scan For Printers

The Smart3 can only be used with Bluetooth Mobile Printer (Code 5-0066).

1. Turn on the power to the Bluetooth Mobile Printer (Code 5-0066).

Editing/Set Up

SMART3 Colorimeter 09.16 53

2. From the Editing Menu, press or to scroll to Bluetooth Menu.

Editing Menu

Factory Setup

Set Power Save

Set Backlight Time

Bluetooth Menu

12:00:00 001/500 P

3. Press

ENTER

to select Bluetooth

Menu.

Editing/Set Up

4. Press or to scroll to

Bluetooth Scan.

5. Press

ENTER

to select Bluetooth Scan. The meter will begin scanning. A count down timer will begin counting down from 11 seconds.

If “No BT Available…” is displayed, Bluetooth is not enabled on the meter. Go to Bluetooth Power to enable it.

Bluetooth Menu

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

Bluetooth Menu

Bluetooth Power

Bluetooth Mode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

Bluetooth Scan

Scanning (11)...

12:00:00 001/500 P

54 SMART3 Colorimeter 09.16

6. The addresses of the available printers will be displayed. NOTE: The printer address for the Bluetooth Mobile Printer (Code 5-0066) can be determined by turning the power oﬀ and then pressing the Power button and the Paper Feed button simultaneously to print the printer speciﬁcations. The printer address is designated as Bluetooth: BT Address.

Select Printer

000D163140A0

Set Backlight Time

12:00:00 001/500 P

Editing/Set Up

7. Scroll to the desired printer and press ENTER to pair the printer. The screen will display the BT Address and Working… for about 1 second. The meter will display

000D163140A0

Connecting

Connect to Printer

a status message when the printer is connected.

12:00:00 001/500 P

Set BT Timeout

The Bluetooth Timeout can be set to allow the Bluetooth feature to be on to allow data to be sent to the Bluetooth printer and then turn oﬀ after a speciﬁed amount of time.

1. From the Editing Menu, press or

to scroll to Bluetooth Menu.

Factory Setup

Set Power Save

Set Backlight Time

Bluetooth Menu

12:00:00 001/500 P

Editing Menu

SMART3 Colorimeter 09.16 55

2. Press

ENTER

to select Bluetooth Menu.

Bluetooth Menu

Bluetooth Power

BluetoothMode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

3. Press or to scroll to Set BT

Timeout.

Editing/Set Up

4. Press

ENTER

to select Set BT Timeout.

5. Press or to scroll to desired option.

Bluetooth Menu

Bluetooth Power

BluetoothMode

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

Set BT Timeout

15 minutes

5 minutes

30 Seconds

Disable

12:00:00 001/500 P

Set BT Timeout

15 minutes

5 minutes

30 Seconds

Disable

12:00:00 001/500 P

6. Press

ENTER

. The screen will display Storing… for about 1 second and the meter will return to the Bluetooth Menu.

Bluetooth Power

Bluetooth Mode

Bluetooth Menu

Bluetooth Scan

Set BT Timeout

12:00:00 001/500 P

56 SMART3 Colorimeter 09.16

 SELECTING A LANGUAGE

There are seven languages available in the Smart3: English, Spanish, French, Portuguese, Italian, Chinese, and Japanese.

1. From the Editing Menu, press or to scroll to Select Language.

2. Press

ENTER

to select Select

Language.

3. Press or to scroll to desired

language.

Editing Menu

Set Power Save

Set Backlight Time

Bluetooth Menu

Select Language

12:00:00 001/500

Select Language

English

Spanish

French

Portugese

12:00:00 001/500

Select Language

English

Spanish

French

Portugese

12:00:00 001/500

Editing/Set Up

4. Press

ENTER

. The screen will display Storing... for about 1 second and the meter will return to the Editing Menu.

Set Power Save

Set Backlight Time

Editing Menu

Bluetooth Menu

Select Language

12:00:00 001/500

NOTE: If meter unintentionally switches to another language, use the procedure above to reset the meter to the desired language. For example, to reset the meter to English:

Turn meter on.

1. Press one time. Press

2. Press eight times. Press

3. Press

SMART3 Colorimeter 09.16 57

ENTER

.

ENTER

.

 LOOPING MENUS

Long menus, such as All Tests, incorporate a looping feature which allows the user to quickly reach the last choice in the menu from the ﬁrst choice. In a looping menu the last choices in the menu are above the ﬁrst choice and scrolling upward moves through the menu in reverse order. Scrolling downward moves through the menu from ﬁrst choice to last but the menu starts over following the last choice. So all menu choices can be reached by scrolling in either direction. The diagrams below demonstrate a looping menu.

AND SO ON AND SO ON AND SO ON

: : : : : : : : :

THIRD TO LAST LAST CHOICE LAST CHOICE

SECOND TO LAST AND SO ON AND SO ON

LAST CHOICE : : : : : :

TESTING MENU TESTING MENU TESTING MENU

FIRST CHOICE

Editing/Set Up

SECOND CHOICE THIRD TO LAST THIRD TO LAST

THIRD CHOICE

ANOTHER LAST CHOICE LAST CHOICE

12:00:00 001/500 12:00:00 001/500 12:00:00 001/500

AND ANOTHER FIRST CHOICE FIRST CHOICE

AND SO ON SECOND CHOICE SECOND CHOICE

: : : THIRD CHOICE THIRD CHOICE

: : : ANOTHER ANOTHER

LAST CHOICE AND ANOTHER AND ANOTHER

The feature called Looping Menu can be turned on and oﬀ in the Editing Menu. The default setting is ON.

: : : : : :

SECOND TO LAST SECOND TO LAST

COMPUTER CONNECTION

 OUTPUT

USB

 COMPUTER CONNECTION

USB Type A, USB mini B, Order Cable Code 1720-01.

 BLUETOOTH CONNECTION

Barriers to wireless signals can reduce the range of wireless devices. The Smart3 will work best if there are no walls between it and the receiving device.

The Smart3 colorimeter uses Bluetooth wireless technology to allow communication between the colorimeter and a bluetooth printer (Code 3-0066 only).

58 SMART3 Colorimeter 09.16

 WATERLINK CONNECT

The meter may be interfaced with any Windows-based 64 bit computer by using the LaMotte WaterLink Connect program and a USB cable. The program will store test information and results in a local database, and allow for exporting this data to a comma separated value (CSV) ﬁle. The meter will send the following data: time/date stamp, name of test, sample value, sample units, meter name, and location. To download WaterLink go to http://softwarecenter.lamotte.com/. Select “WaterLink Connect Software for Windows FREE Download”.

BATTERY

 BATTERY/AC OPERATION

The Smart3 may be operated on battery power using a USB wall adapter or USB computer connection. If using the meter as a bench top unit, use the wall adapter if possible to extend the battery life. The meter will remain on when the USB adapter is used.

To charge the battery with the wall adapter, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB type A connector) into the wall adapter. Plug the wall adapter into an AC outlet. Reinsert the rubber USB port plug after charging. To charge the battery from a computer, plug the smaller end of the USB cable (USB mini B connector) into the meter and the larger end of the USB cable (USB Type A connector) into a USB port on a computer. The battery icon will show no bars and ﬂash when the unit ﬁrst turns on. Then the indicator will indicate the battery status by showing 0, 1, 2, 3, or 4 bars.

It will take 6 hours to fully charge a low battery. The battery icon will ﬂash when the battery is charging. The battery icon will show four bars and stop ﬂashing when it is fully charged. The charging circuit will automatically switch to a ﬂoat charge when the battery is fully charged. The charger may remain connected. Some computers will NOT supply power to their USB ports during standby operation. The wall adapter will charge the unit continuously.

Storing the meter above ambient room temperature will decrease the battery charge more quickly than storage at room temperature. If the meter does not turn on, it means that the battery is at a very low charge. Charging the battery with the wall adapter in this state may take up to 10 hours. At low temperatures, approaching 0 °C, the battery will charge more slowly. It will not charge at all below 0 °C. The battery icon will show no bars and continuously ﬂash if the battery is getting low but the unit will still operate normally. A “Low Battery” message on the status bar of the display will replace the time when the battery voltage is too low for proper operation and accuracy may be degraded. A “Shutdown Low Batt” message on the display will appear for a few seconds before the power is switched oﬀ when the battery is too low to operate the unit. When the battery icon simultaneously ﬂashes bars 1 and 2 followed by bars 3 and 4, it is an indication that the battery is damaged and technical support should be contacted.

To extend the battery life:

◊ Shut down the unit with the power switch when not taking measurements or use the

power save option to have the unit automatically turn oﬀ after 5 minutes.

◊ Store the unit at a moderate temperature.

Editing/Set Up

SMART3 Colorimeter 09.16 59

◊ Fully charge the battery before storing the unit for extended periods of time. ◊ Fully charge the battery at least once per year. Failure to do so may result in a

permanently drained battery.

◊ Limit backlight use. The unit consumes 3X normal power with the backlight on. Set

the backlight time option to 10 seconds, or select “Button Control” and keep the backlight oﬀ.

 BATTERY REPLACEMENT

The lithium ion battery used in this unit should last for many years with normal use. When it no longer powers the unit long enough to meet testing requirements it will need to be replaced. Lithium ion batteries that are properly charged and stored do not usually lose all capacity; they just have less capacity after hundreds of charge cycles. This unit uses a custom battery assembly that is only available from LaMotte Company. Battery replacement must be performed at a LaMotte authorized repair facility. The water resistant housing of this meter should not be opened by the user. Contact LaMotte Company by phone (1-800-344-3100) or email (tech@lamotte.com) for a return authorization number.

MAINTENANCE

Editing/Set Up

 CLEANING

Clean the exterior housing with a damp, lint-free cloth. Do not allow water to enter the light chamber or any other parts of the meter. To clean the light chamber and optics area, point a can of compressed air into the light chamber and blow the pressurized air into the light chamber. Use a cotton swab dampened with Windex® window cleaner to gently swab the interior of the chamber. Do not use alcohol; it will leave a thin residue over the optics when dry.

 REPAIRS

Should it be necessary to return the meter for repair or servicing, pack the meter carefully in a suitable container with adequate packing material. A return authorization number must be obtained from LaMotte Company by calling 800-344-3100 (US only) or 410778-3100, faxing 410-778-6394, or emailing tech@lamotte.com. Often a problem can be resolved over the phone or by email. If a return of the meter is necessary, attach a letter with the return authorization number, meter serial number, a brief description of problem and contact information including phone and FAX numbers to the shipping carton. This information will enable the service department to make the required repairs more eﬃciently.

 METER DISPOSAL

Waste Electrical and Electronic Equipment (WEEE) Natural resources were used in the production of this equipment. This equipment may

contain materials that are hazardous to health and the environment. To avoid harm to the environment and natural resources, the use of appropriate take-back systems is

60 SMART3 Colorimeter 09.16

recommended. The crossed out wheeled bin symbol on the meter encourages the use of these systems when disposing of this equipment.

Take-back systems will allow the materials to be reused or recycled in a way that will not harm the environment. For more information on approved collection, reuse, and recycling systems contact local or regional waste administration or recycling services.

TROUBLESHOOTING

 ERROR MESSAGES

OVER RANGE

If the message OVERRANGE is displayed when scanning a sample, the sample may be over range or under range. If the sample is over range the sample should be diluted and tested again (see Sample Dilution Techniques and Volumetric Measurements, page 19).

If overrange is displayed, press continue testing on diluted samples.

Note: After pressing cncentration will be displayed. This concentration is an approximation only.

ENTER

to

, the overrange

Overrange

<Enter> continue

Scan Blank

Scan Sample

12:00:00 001/500

CALIBRATION

As with all pre-calibrated meters, it is highly recommended, even if not required by regulations, that the user periodically verify the performance of the meter by running standards with a predetermined concentration. Results outside of speciﬁcation are an indication that the meter needs to be adjusted. This can be done following the user calibration described on page 28. If the user calibration fails to properly adjust the meter then the meter should be returned to LaMotte Company for recalibration. (See page 73).

STRAY LIGHT

The Smart3 Colorimeter should have no problems with stray light. Make sure that the sample compartment lid is always fully closed, except when testing COD with the adapter.

 TROUBLESHOOTING GUIDE

005 Aluminum

Editing/Set Up

PROBLEM REASON SOLUTION

Flashing

“Low Battery” Battery voltage is very low.

“Shut Down Low Batt” Shut Down

SMART3 Colorimeter 09.16 61

Low battery. Readings are reliable.

Readings are not reliable.

Battery is too low to operate the unit.

Charge battery or use USB wall/computer adapter.

“Overrange” Sample is outside of

acceptable range.

Dilute sample and test again.

Unusually large negative or positive

Incorrect standards used to

calibrate meter. readings when performing calibration

 SOFTWARE TROUBLESHOOTING

PROBLEM REASON SOLUTION

No printer connected Colorimeter has not been

paired via Bluetooth, or pairing

was lost by leaving Bluetooth

range.

Printer does not appear in the list of Bluetooth devices

Editing/Set Up

Connection lost Bluetooth pairing with the

Printer in not powered on. Power on the printer

printer was lost.

Use fresh 0.0 standard in clean tube. Reset meter to factory default settings. Recalibrate meter.

Re-pair the colorimeter to the printer.

Re-pair the Printer to the colorimeter

62 SMART3 Colorimeter 09.16

SMART3

Colorimeter

Test

Procedures

1910-Test-V3.indd

SMART3 COLORIMETER REAGENT SYSTEMS

SMART3 REAGENT SYSTEMS LIST

LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available.

Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or email at tech@lamotte.com for a current list of available calibrations and more information. Some reagent systems are not available for shipment to Canada.

# of

Test Factor (Test #) Range (ppm) MDL Test Method (# of Reagents)

Alkalinity-UDV (002) 0–250 10 Unit Dose Vials (1) 100

Aluminum (005) † 0.00–0.30 0.01 Eriochrome Cyanine R (4) 50

Ammonia Nitrogen- Low Range, Fresh Water (006)

Ammonia Nitrogen- Low Range, Salt Water (007)

Ammonia Nitrogen- High Range (008)

Barium (009) 0-200 5 Barium Chloride (1) 50

Biguanide (011) 0–70 2 Colorimetric (1) 50

Borate-UDV (013) 0–80 5 Unit Dose Vial (1) 100

Boron (014) 0.00-0.80 0.05 Azomethine-H (2) 50

Bromine, Liquid DPD (015) 0.00-9.00 0.10 DPD, Liquid (3) 144

Bromine, Tablet (016) † 0.00–9.00 0.10 DPD (3) 100

Bromine-UDV (017) 0.0–22.0 0.1 DPD (1) 100

Cadmium (018) 0.00–1.00 0.02 PAN (4) 50

Carbohydrazide (019)

See Oxygen Scavengers

Chloride-TesTab (021) † 0.0–30.0 0.4 Argentometric (1) 50

Chlorine-Liquid DPD (024) 0.00–4.00 0.03 DPD (3) 144

Chlorine-Tablet DPD (025) † 0.00–4.00 0.03 DPD (3) 100

Chlorine-Free-UDV (026) 0.00–10.00 0.10 DPD (1) 100

Chlorine-Total-UDV (027) 0.00–10.00 0.10 DPD (1) 100

Chlorine Dioxide (022) 0.00–8.00 0.06 DPD/Glycine (2) 100

Chromium, Hexavalent (028) 0.00–1.00 0.01 Diphenylcarbohydrazide (1) 50

Chromium, Hex, Tri, Total (028) 0.00–1.00 0.01 Diphenylcarbohydrazide (5) 50

Cobalt (029) 0.00–2.00 0.04 PAN (3) 50

COD-Low Range (031) † 0–150 5 Digestion (1) 25

COD-Standard Range (032) 0-1500 50 Digestion (1) 25

0.00–1.00 0.05 Salicylate (3) 25

0.00–1.00 0.10 Salicylate (3) 25

0.00–4.00 0.05 Nesslerization (2) 50

0.000–0.900 0.04 Iron Reduction (3) 100

Tests

†Available for shipment to Canada

COD-High Range (030) † 0–15000 500 Digestion (1) 25

Color (033) 0–1000 cu 20 Platinum Cobalt (0) –

Copper-BCA (034) 0.00–3.50 0.04 Bicinchoninic Acid (1) 50

Copper-Cuprizone (035) 0.00–2.00 0.03 Cuprizone (2) 50

Copper-Thiocarbamate (036) 0.00–6.00 0.10 Diethyldithiocarbamate (1) 50

Copper-UDV (037) 0.0–4.0 0.1 Bicinchoninic Acid (1) 100

Cyanide (038) 0.00-0.35 0.03 Pyridine-Barbituric Acid (5) 50

Cyanuric Acid (039) 5–200 10 Melamine (1) 50

Cyanuric Acid-UDV (041) 5–150 10 Melamine (1) 100

DEHA (042) See Oxygen Scavengers

Dissolved Oxygen (043) 0.0–11.0 0.6 Winkler Colorimetric (3) 100

Erythorbic Acid (044) See Oxygen Scavengers

Fluoride (045) † 0.0–2.0 0.1 SPADNS (2) 50

Hardness (Total) UDV (048) 0–500 10 Unit dose Vial (1) 100

Hydrazine (049) 0.00–1.00 0.01 P-dimethyl-

Hydrogen Peroxide- Low Range (051)

Hydrogen Peroxide- High Range (050)

Hydrogen Peroxide-Shock (052) 0–225 10 DPD (2) 100

Hydroquinone (053) See Oxygen Scavengers

Iodine (054) † 0.0–14.0 0.2 DPD (2) 100

Iron-Bipyridyl (055) † 0.00–6.00 0.10 Bipyridyl (2) 50

Iron-Total, Ferrous, Ferric (056) † 0.0–5.0 0.1 1,10 Phenanthroline (2) 50

Iron-UDV (057) 0.0–10.0 0.1 Bipyridyl (1) 100

Lead (058) 0.0–5.0 0.1 PAR (5) 50

Manganese-Low Range (060) † 0.00–0.70 0.01 PAN (3) 50

Manganese-High Range (059) † 0.0–15.0 0.3 Periodate (2) 50

Methylethylketoxime (062) See Oxygen Scavengers

Molybdenum-High Range (063) 0.0–50.0 0.6 Thioglycolate (3) 50

Nickel (064) 0.00–8.00 0.15 Dimethylglyoxime (6) 50

Nitrate Nitrogen-Low Range (065) † 0.00–3.00 0.10 Cadmium Reduction (2) 20

Nitrate TesTab (066) † 0-60 5 Zinc Reduction (1) 50

Nitrate-UDV (067) 0-80 2 Zinc Reduction (1) 100

Nitrite Nitrogen-Low Range (068) † 0.00–0.80 0.02 Diazotization (2) 20

†Available for shipment to Canada

0.00–0.70 0.01 Iron Reduction (3) 100

0.00–3.00 0.02 Iron Reduction (3) 100

aminobenzaldehyde (2)

0.00–1.50 0.02 DPD (2) 100

0–60 1 DPD (2) 100

0.00–2.00 0.01 Iron Reduction (3) 100

0.00–3.00 0.01 Iron Reduction (3) 100

50

Nitrogen, Total*** (069) 0-25 mg/L 3 mg/L Chromotropic Acid/Digestion

(6)

Oxygen Scanvengers various various DEHA (3) 50

Ozone-DPD Liquid (073) 0.00–3.00 0.03 DPD (4) 144

Ozone-Low Range (071) 0.00–0.40 0.01 Indigo Trisulfonate (3) 100

Ozone-High Range (072) 0.00–2.50 0.05 Indigo Trisulfonate (3) 20

pH-Chlorophenol Red (074) † 5.0–6.8 – Chlorophenol Red (1) 100

pH-Phenol Red (075) † 6.6–8.4 – Phenol Red (1) 100

pH-Thymol Blue (077) † 8.0–9.5 – Thymol Blue (1) 100

Phenol (079) 0.00-6.00 0.05 Aminoabtipyrine (2) 50

Phosphate-Low Range (081) † 0.00–3.00 0.05 Ascorbic Acid Reduction (2) 50

Phosphate-High Range (080) † 0.0–70.0 0.5 Vanodomolybd-

phosphoric Acid (1)

Phosphorus, ppb (082) 0–3000 ppb 50 Ascorbic Acid/Reduction (5) 50

Phosphorus, Total, Low Range*** (084)

Phosphorus, Total, High-Range*** (083)

Potassium (085) † 0.0-10.0 0.8 Tetraphenylboron (2) 100

Silica-Low Range (087) 0.00–4.00 0.05 Heteropoly Blue (4) 50

Silica-High Range (086) 0–75 1 Silicomolybdate (3) 50

Sulfate-High Range (089) † 0–100 3 Barium Chloride (1) 50

Sulﬁde-Low Range (090) 0.00–1.50 0.06 Methylene Blue (3) 50

Surfactants (092) 0.0-8.0 0.5 Bromphenol Blue (3) 100

Tannin (093) 0.0–10.0 0.1 Tungsto-molybdophosphoric

Turbidity (095) 0–400 FAU 3 Absorption (0) –

Zinc-Low Range (097) † 0.00–3.00 0.05 Zincon (6) 50

***Requires COD Adapter Code 5-0087 and Heater Block. †Available for shipment to Canada

0.00–3.50 mg/L

5-100 mg/L 5 Molybdovanadate/Digestion (5) 25

0.50 Ascorbic Acid/Digestion 25

Acid (2)

25

50

ALKALINITY–UDV

UNIT DOSE VIALS • CODE 4318-J

QUANTITY CONTENTS CODE

1 *Alkalinity Unit Dose Vials, 20 pouches *4318-J

Equipment needed but not supplied:

STANDARD ACCESSORY PACKAGE · CODE 1961

1 Package of 3 Vials (empty) 0156

1 Syringe, 3 mL, plastic 1184

1 Foil Storage Bag 9467

Or:

ADVANCED ACCESSORY PACKAGE · CODE 1962

1 Pipettor, 3mL, w/tip 30529-BRAND

1 Cuvette Rack 31695

1 Package of 3 Vials (empty) 0156

1 Foil Storage Bag 9467

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Test Procedures

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

APPLICATION: Drinking and surface waters; swimming pool water.

RANGE: 0–250 ppm as CaCO

MDL: 10 ppm

METHOD The sample is added to a buﬀered indicator reagent. The

color that develops, ranging from yellow to blue, will indicate the amount of alkalinity in the sample.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Quats and poly quats at high concentrations will interfere.

Smart3 Test Procedures 09.16 ALKALINITY, UDV

Samples should be analyzed as soon as possible after collection. Sample may be refrigerated for 24 hours.

3

PROCEDURE

Use COD/UDV adapter

1. Press and hold until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 002 Alkalinity UDV) from Testing

4. Scroll to and select 002 Alkalinity UDV from menu.

5. Rinse a clean vial (0156) with sample water.

6. Use the syringe (1184) to add 3 mL of sample to the vial.

7. Insert the vial into chamber, close lid and select Scan Blank.

8. Remove vial from the colorimeter.

9. Use the syringe (1184) to add 3 mL of sample to an *Alkalinity-UDV vial

10. Wait 90 seconds.

11. Invert vial 3 times to mix.

12. Insert tube into chamber, close lid and select Scan Sample. Record result.

13. Press to turn the colorimeter oﬀ or press

NOTES: For best possible results, a reagent blank should be determined to account for

Test Procedures

any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

ENTER

to select Testing Menu.

Menu.

(4318).

NOTE: If powder residue remains in the bottom of the vial after inverting, invert once more and tap bottom of vial sharply once or twice to dislodge powder. Mix.

EXIT

to exit to a previous menu

or make another menu selection.

UDVs from opened pouches should be used promptly. Store unused vials from opened pouches in the Foil Storage Bag (9467) to extend the shelf life of the reagent. Generally, UDVs stored in the bag should be used within 10 days if the humidity is less than 50% and within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced.

ALKALINITY, UDV Smart3 Test Procedures 09.16

ALUMINUM

ERIOCHROME CYANINE R METHOD • CODE 364I-01-SC

QUANTITY CONTENTS CODE

5 g Aluminum Inhibitor Reagent 7865-C

2 x 120 mL *Aluminum Buﬀer Reagent *7866-J

120 mL Aluminum Indicator Reagent 7867-J

15 mL Aluminum Complexing Reagent 7868-E

1 Spoon, 0.05 g, plastic 0696

2 Pipets, 1.0 mL, plastic 0354

1 Test Tube, glass, 5 mL w/cap 0230

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Aluminum is the third most common element in the earth’s crust, which accounts for its wide appearance in many water supplies. Aluminum exists in water as soluble salts, colloidal compounds, and insoluble compounds. In wastewater that has been treated by alum coagulation it will appear in one or more of the above forms. Properly treated drinking water should have an aluminum concentration below 0.05 mg/L.

Test Procedures

APPLICATION: Drinking, surface, and saline waters; domestic and

industrial wastewater.

RANGE: 0.00–0.30 ppm Aluminum

MDL: 0.01 ppm

METHOD: Aluminum ions buﬀered to a pH of 6.0 react with

Eriochrome Cyanine R dye to produce a pink to red complex in proportion to the concentration.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Fluoride and polyphosphate will interfere. Interference from

Smart3 Test Procedures 11.18 ALUMINUM

Collect sample in acid washed glass or plastic bottle. Analyze as soon as possible.

iron and manganese is eliminated by the addition of an inhibitor.

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 005 Aluminum) from Testing

4. Scroll to and select 005 Aluminum from menu.

5. Rinse a clean colorimeter tube (0290) with sample water. Fill to the 10 mL line

6. Insert tube into colorimeter chamber and select Scan Blank.

7. Rinse a clean test tube (0230) with sample water. Fill to the 5 mL line with

8. Remove tube from colorimeter. Empty sample from tube (0290).

9. Add 5 mL sample from test tube (0230) to empty tube (0290).

10. Use the 0.05 g spoon (0696) to add one measure of Aluminum Inhibitor Reagent

11. Use a 1.0 mL pipet (0354) to add 2 mL of *Aluminum Buﬀer Reagent (7866). Cap

12. Use a second 1.0 mL pipet (0354) to add 1 mL of Aluminum Indicator

13. At end of 5 minute waiting period, mix, insert tube into chamber, close lid and

14. Press to turn the colorimeter oﬀ or press

Test Procedures

NOTE: For the best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Add 5 drops of Aluminum Complexing Reagent (7868). Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

ENTER

to select Testing Menu.

Menu.

with sample.

sample.

(7865). Cap and mix to dissolve powder.

and mix.

Reagent (7867). Cap and mix contents. Wait 5 minutes for maximum color development.

select Scan Sample. Record result.

EXIT

to exit to a previous menu

or make another menu selection.

ALUMINUM Smart3 Test Procedures 11.18

AMMONIA NITROGEN - LOW RANGE

SALICYLATE METHOD • CODE 3659-01-SC

QUANTITY CONTENTS CODE

60 mL *Salicylate Ammonia #1 *3978-H

10 g *Salicylate #2 Reagent *7457-D

2 x 5 g *Salicylate #3 Reagent Powder *7458-C

1 Spoon, 0.1 g, plastic 0699

1 Spoon, 0.15 g, plastic 0727

1 Pipet, 1.0 mL, plastic 0354

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Ammonia nitrogen is present in various concentrations in many surface and ground water supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply is cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes accepted as chemical evidence of pollution when encountered in natural waters.

Test Procedures

Ammonia is rapidly oxidized in natural water systems by special bacterial groups that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in the water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to the expanded growth of undesirable algae and other forms of plant growth that overload the natural system and cause pollution.

Smart3 Test Procedures 09.16 AMMONIA NITROGEN, Low Range

APPLICATION: Low concentrations of ammonia in fresh, brackish and salt

water; fresh and salt water aquariums.

RANGE: 0.00 - 1.00 ppm Ammonia-Nitrogen

MDL: 0.05 ppm Fresh Water

0.10 ppm Salt Water

METHOD: Salicylate and ammonia react at high pH in the presence of a

chlorine donor and an iron catalyst to form a blue indophenol dye, the concentration of which is proportional to the ammonia concentration in the sample.

SAMPLE HANDLE & PRESERVATION:

Ammonia solutions tend to be unstable and should be analyzed immediately. Samples may be stored for 24 hours at 4°C or 28 days at –20°C.

INTERFERENCES: There are few interferences in most natural waters. High

concentrations of reducing agents, such as hydrazine, react with the chlorine donor and can result in negative interferences. Color and turbidity can also interfere.

Test Procedures

AMMONIA NITROGEN, Low Range Smart3 Test Procedures 09.16

PROCEDURE - FRESH WATER

1. Press and hold until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 006 Ammonia-N LRF) from

4. Scroll to and select 006 Ammonia-N LRF from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with

6. Insert tube into chamber, close lid and select Scan Blank. (See Note.)

7. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL

8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent

9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures

10. At the end of the 12 minute waiting period, immediately mix and insert tube into

11. Press to turn colorimeter oﬀ or press

ENTER

to select Testing Menu.

Testing Menu.

sample.

of *Salicylate Ammonia #1 (3978). Cap and mix.

(7457). Cap and mix until dissolved. Wait 1 minute.

of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least 30 seconds and all solid has dissolved. Wait 12 minutes for maximum color development.

chamber, close lid and select Scan Sample. Record result.

EXIT

to exit to a previous menu or

make another menu selection.

Test Procedures

CALCULATIONS:

To express results as Unionized Ammonia (NH3):

ppm Unionized Ammonia (NH3) =

ppm Ammonia-Nitrogen (NH3–N) x 1.2

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

+

) =

4

ppm Ammonia-Nitrogen (NH3–N) x 1.3

To determine the percentages of Unionized and Ionized Ammonia-Nitrogen, consult the Appendix.

NOTE: It is strongly suggested that a reagent blank be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

Smart3 Test Procedures 09.16 AMMONIA NITROGEN, Low Range

PROCEDURE - SALT WATER

1. Press and hold until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 007 Ammonia-N LRS) from

4. Scroll to and select 007 Ammonia-N LRS from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with

6. Insert tube into chamber, close lid and select Scan Blank. (See Note.)

7. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL

8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent

9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures

10. At the end of the 20 minute waiting period, immediately mix and insert tube into

11. Press to turn colorimeter oﬀ or press

ENTER

to select Testing Menu.

Testing Menu.

sample.

of *Salicylate Ammonia #1 (3978). Cap and mix.

(7457). Cap and mix until dissolved. Wait 1 minute.

of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least 30 seconds and all solid has dissolved. Wait 20 minutes for maximum color development.

chamber, close lid and select Scan Sample. Record result.

EXIT

to exit to a previous menu or

make another menu selection.

CALCULATIONS:

Test Procedures

To express results as Unionized Ammonia (NH3):

ppm Unionized Ammonia (NH3) =

ppm Ammonia-Nitrogen (NH3–N) x 1.2

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

4

+

) =

ppm Ammonia-Nitrogen (NH3–N) x 1.3

To determine the percentages of Unionized and Ionized Ammonia-Nitrogen, consult the Appendix.

NOTE: It is strongly suggested that a reagent blank be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

AMMONIA NITROGEN, Low Range Smart3 Test Procedures 09.16

AMMONIA NITROGEN - HIGH RANGE

NESSLERIZATION METHOD • CODE 3642-SC

QUANTITY CONTENTS CODE

30 mL Ammonia Nitrogen Reagent #1 V-4797-G

2 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G

1 Pipet, 1 mL, plastic 0354

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Ammonia nitrogen is present in various concentrations in many surface and ground water supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply is cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes accepted as chemical evidence of pollution when encountered in natural waters.

Ammonia is rapidly oxidized in natural water systems by special bacterial groups that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in the water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to the expanded growth of undesirable algae and other forms of plant growth that overload the natural system and cause pollution.

Test Procedures

Smart3 Test Procedures 09.16 AMMONIA NITROGEN, High Range

APPLICATION: Drinking, surface, and saline waters; domestic and

industrial wastes.

RANGE: 0.00–4.00 ppm Ammonia Nitrogen

MDL: 0.05 ppm

METHOD: Ammonia forms a colored complex with Nessler’s

Reagent in proportion to the amount of ammonia present in the sample. Rochelle salt is added to prevent precipitation of calcium or magnesium in undistilled samples.

SAMPLE HANDLING & PRESERVATION:

Ammonia solutions tend to be unstable and should be analyzed immediately. Sample may be stored for 24 hours at 4°C or 28 days at –20°C.

INTERFERENCES: Sample turbidity and color may interfere. Turbidity may

be removed by a ﬁltration procedure. Color interference may be eliminated by blanking the instrument with a sample blank.

Test Procedures

AMMONIA NITROGEN, High Range Smart3 Test Procedures 09.16

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Scroll to and select All Tests (or another sequence containing 008 Ammonia-N HR)

4. Scroll to and select 008 Ammonia-N HR from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber, close lid and select Scan Blank. (See Note)

7. Remove tube from colorimeter. Add 8 drops of Ammonia Nitrogen Reagent #1 (V-

8. Use the 1.0 mL pipet (0354) to add 1.0 mL of *Ammonia Nitrogen Reagent #2 (V-

9. At end of the 5 minute waiting period, immediately mix, insert tube into chamber,

10. Press to turn the colorimeter oﬀ or press the

CALCULATIONS:

ENTER

to select Testing Menu.

from Testing Menu.

4797). Cap and mix. Wait 1 minute.

4798). Cap and mix. Allow 5 minutes for maximum color development.

close lid and select Scan Sample. Record result.

EXIT

exit to a previous menu

or make another menu selection.

To express results as Unionized Ammonia (NH3):

ppm Unionized Ammonia (NH3) =

ppm Ammonia-Nitrogen (NH3–N) x 1.2

To express results as Ionized Ammonia (NH4):

ppm Ionized Ammonia (NH

4

+

) =

ppm Ammonia-Nitrogen (NH3–N) x 1.3

To determine the percentages of Unionized and Ionized Ammonia-Nitrogen, consult the Appendix.

NOTE: It is strongly suggested that a reagent blank be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

Smart3 Test Procedures 09.16 AMMONIA NITROGEN, High Range

Test Procedures

AMMONIA NITROGEN, High Range Smart3 Test Procedures 09.16

BARIUM

BARIUM CHLORIDE METHOD • CODE 3638-SC

QUANTITY CONTENTS CODE

50 g Barium Reagent Powder 6330-H

1 Spoon, 0.5g, plastic 0698

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Barium is a naturally occurring metal that is found in rocks and, in trace amounts, in natural waters. A barium concentration above 2 ppm in drinking water is a classiﬁed as a contaminant. Barium sulfate is used in the medical ﬁeld as an X-ray radio contrast agent for imaging the gastrointestinal tract. Barium has many industrial applications. It is used in the manufacture of paints, brakes, root canal ﬁllings, glass, and motor oil detergents. Barium nitrate imparts a bright yellow-green color to ﬁreworks and ﬂares. Barium sulfate is a component of oil well drilling mud which is used to lubricate drill bits. Barium is also a constituent of some electro ceramics and high temperature yttrium barium copper oxide (YBCO) superconductors.

Test Procedures

APPLICATION: Industrial

RANGE: 0 – 200 ppm barium

MDL: 5 ppm

METHOD: Barium is precipitated in an acid medium with sodium

sulfate to form a barium sulfate suspension in proportion to the amount of barium present.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: Suspended matter and color interference may be

Smart3 Test Procedures 09.16 BARIUM

Barium samples may be preserved by refrigeration at 4°C up to 7 days in glass or plastic containers without any change in concentration.

removed by a ﬁltration step. Silica in excess of 500 mg/L will interfere. Strontium will interfere. Check for stray light interference (see page 20).

PROCEDURE

11. Press and hold until colorimeter turns on.

12. Press

13. Select All Tests (or another sequence containing 009 Barium) from Testing

14. Scroll to and select 009 Barium from menu.

15. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample.

16. Insert the tube into chamber, close the lid and select Scan Blank.

17. Remove the tube from the colorimeter.

18. Use the 0.5 g spoon (0698) to add one measure of Barium Reagent Powder (6330).

19. Insert tube into chamber, close lid and select Scan Sample. Record result in ppm

20. Press to turn the colorimeter oﬀ or press

NOTE: A while ﬁlm is deposited on the inside of test tubes as a result of the barium test. Thoroughly clean and rinse test tubes after each test.

ENTER

to select Testing Menu.

Menu.

Cap and mix by inverting for one minute.

barium.

make another menu selection.

EXIT

to exit to a previous menu or

For the most accurate results, samples and reactions should be at 25 ±4°C.

Test Procedures

BARIUM Smart3 Test Procedures 09.16

BIGUANIDE

COLORIMETRIC METHOD • CODE 4044

QUANTITY CONTENTS CODE

2 X 60 mL Biguanide Indicator 3994-H

1 Pipet, plastic, 1.0 mL 0354

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Biguanide is a non-chlorine, non-bromine chemical sanitizer. It is more stable than chlorine or bromine and has little chemical odor. Biguanide is an eﬀective bacteriacide but, unlike chlorine and bromine, it does not destroy organic contaminants. Therefore, hydrogen peroxide is added to biguanide pools on a regular basis to eliminate organic contaminants. The optimum recommended level of biguanide is 30 to 50 ppm.

APPLICATION: Swimming pools

RANGE: 0–70 ppm Biguanide

MDL: 2 ppm

METHOD: Biguanide complexes with the proprietary indicator

to produce a colored solution. The color ranges from yellow through green to blue depending on the biguanide concentration.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCES: The only interfering substances that are likely to be

Smart3 Test Procedures 09.16 BIGUANIDE

Samples should be analyzed as soon as possible.

encountered in pool water are oxidized manganese and oxidizing agents, such as chlorine, bromine and ozone.

Test Procedures

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 011 Biguanide) from Testing

4. Scroll to and select 011 Biguanide from menu.

5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample.

6. Insert the tube into chamber, close lid and select Scan Blank.

7. Remove the tube from colorimeter.

8. Use the 1.0 mL pipet (0354) to add 2.0 mL of Biguanide Indicator (3994). Cap and

9. Wait 1 minute.

10. Insert the tube into chamber. Close lid.

11. Select Scan Sample. Record result in ppm Biguanide

12. Press to turn the colorimeter oﬀ or press

NOTE: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents is obtained.

Test Procedures

ENTER

to select Testing Menu.

Menu.

invert three times to mix.

make another menu selection.

EXIT

to exit to a previous menu or

BIGUANIDE Smart3 Test Procedures 09.16

BORATE - UDV

UNIT DOSE VIALS • CODE 4322-J

QUANTITY CONTENTS CODE

100 *Borate UDV, 20 pouches *4322-J

Equipment needed but not supplied:

STANDARD ACCESSORY PACKAGE · CODE 1961

1 Package of 3 Vials (empty) 0156

1 Syringe, 3 mL, plastic 1184

1 Foil Storage Bag 9467

Or:

ADVANCED ACCESSORY PACKAGE · CODE 1962

1 Pipettor, 3mL, w/tip 30529-BRAND

1 Cuvette Rack 31695

1 Package of 3 Vials (empty) 0156

1 Foil Storage Bag 9467

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Test Procedures

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Some swimming pools use a borate buﬀering system. Borates lower the level of carbon dioxide in the pool, which slows algae growth. This results in a lower chlorine requirement. Free chlorine levels in pools with borate systems can be maintained at 1.0 ppm.

Small amounts of boron are necessary for plant growth but large amounts can be toxic. In humans, boron aids in the uptake of calcium and the production of strong bones. An excess of boron can aﬀect the central nervous system resulting in a syndrome known as borism. Some natural waters may contain small amounts of boron. Large concentrations may be due to industrial eﬄuent entering waterways. Boron compounds are used in cleaning compounds, paper and paints, fertilizers, glass and ceramics, ﬁre retardants and the production of alloys. In the atomic energy ﬁeld, boron is a component of neutron shields and nuclear reactors.

Smart3 Test Procedures 09.16 BORATE, UDV

APPLICATION: swimming pools, surface and saline waters, hydroponic

solutions, industrial waste.

RANGE: 0.00 – 80.0 ppm boron

MDL: 5 ppm

METHOD: Borate reacts with a selective boron indicator powder to

form a colored complex at pH 7.2 to 7.4 in proportion to the concentration of boron present.

SAMPLE HANDLING & PRESERVATION:

Store samples in polyethylene bottles. Do not use borate detergents or glassware.

INTERFERENCES: Interferences in swimming pool water are unlikely.

Test Procedures

BORATE, UDV Smart3 Test Procedures 09.16

PROCEDURE

Use COD/UDV adapter

1. Press until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 013 Borate UDV) from Testing

4. Scroll to and select 013 Borate UDV from menu.

5. Rinse a clean vial (0156) with sample water.

6. Use the syringe (1184) to add 3mL of sample to the vial.

7. Insert the vial into chamber, close the lid and select Scan Blank.

8. Remove the vial from the colorimeter.

9. Use the syringe (1184) to add 3mL of sample to a *Borate UDV vial (4322).

10. Wait 3 minute.

11. Invert the vial three times to mix.

12. Immediately insert tube into chamber, close lid and select Scan Sample. Record

13. Press to turn the colorimeter oﬀ or press

NOTE: For best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank. Then follow the above procedure to perform the test on a distilled or deionized water sample. This test result is the reagent blank. Subtract the reagent blank from all subsequent test results of unknown samples. It is necessary to determine the reagent blank only when a new lot number of reagents are obtained.

ENTER

to select Testing Menu.

Menu.

NOTE: If powder residue remains in the bottom of the vial after inverting or air bubbles form, invert once more and tap bottom of vial sharply once or twice to dislodge powder and bubbles. Mix.

result in ppm borate.

EXIT

to exit to a previous menu or

make another menu selection.

Test Procedures

NOTE: UDVs from opened pouches should be used promptly. Store unused vials from opened pouches in the Foil Storage Bag (9467) to extend the shelf life of the reagent. Generally, UDVs stored in the bag should be used within 10 days if the humidity is less than 50% and within 5 days if humidity is greater than 50%. The Foil Storage Bag contains a desiccant pack with indicator. When the indicator in the window turns from blue to pink, the bag should be replaced.

Smart3 Test Procedures 09.16 BORATE, UDV

Test Procedures

BORATE, UDV Smart3 Test Procedures 09.16

BORON

AZOMETHINE-H METHOD · CODE 4868-01

QUANTITY CONTENTS CODE

120 mL *Boron Buﬀer *4869-J

10 g *Boron Indicator Powder *4870-D

1 Pipet, plastic, 1.0 mL 0354

1 Spoon, 0.15 g 0727

1 Dark storage chamber, brown 0108

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Small amounts of boron are necessary for plant growth but large amounts can be toxic. In humans, boron aids in the uptake of calcium and the production of strong bones. An excess of boron can aﬀect the central nervous system resulting in a syndrome known as borism. Some natural waters may contain small amounts of boron. Large concentrations may be due to industrial eﬄuent entering waterways. Boron compounds are used in cleaning compounds, paper and paints, fertilizers, glass and ceramics, ﬁre retardants and the production of alloys. In the atomic energy ﬁeld, boron is a component of neutron shields and nuclear reactors. Some swimming pools use boron buﬀering systems.

Test Procedures

Smart3 Test Procedures 09.16 BORON

APPLICATION: Surface and saline waters, hydroponic solutions, industrial

waste, swimming pools.

RANGE: 0.00–0.80 ppm Boron

MDL: 0.05

METHOD: Azomethine-H and borate form a yellow complex at pH 6 in

proportion to the concentration of boron present.

SAMPLE HANDLING & PRESERVATION:

Store samples in polyethylene bottles. Do not use borate detergents or glassware.

INTERFERENCES: Interferences in drinking water are unlikely. Manganese,

zirconium, chromium, titanium, copper, vanadium, aluminum, beryllium and iron may cause high results.

Test Procedures

BORON Smart3 Test Procedures 09.16

PROCEDURE

1. This test requires a Reagent Blank. Rinse a tube (0290) with clear, colorless, boron free water. Fill to 10 mL line with clear, colorless, boron free water.

2. Use the 1.0 mL pipet (0354) to add 2 mL of *Boron Buﬀer (4869). Cap and mix.

3. Use the 0.15 g spoon (0727) to add one level measure of *Boron Indicator Powder (4870). Press full spoon against side of jar to compress powder. Scrape oﬀ excess powder on inside neck of bottle. Tap excess oﬀ spoon handle.

4. Cap and shake vigorously for 30 seconds.

5. Insert the tube into meter chamber. Close lid.

6. Start a timer set for 30 minutes. Do not open the lid during the waiting time. The reaction is photosensitive.

7. Rinse a clean tube (0290) with Sample Water. Fill to the 10 mL line with sample water. Repeat steps 2–4.

8. Insert the tube into the Dark Storage Chamber (0108). Close top.

9. Start a second timer set for 30 minutes. Do not open the chamber during the waiting time. The reaction is photosensitive.

10. When 2 minutes remain on the ﬁrst timer (Reagent Blank), press and hold ON button until colorimeter turns on.

11. Press and hold until colorimeter turns on.

12. Press

13. Select All Tests (or another sequence containing 014 Boron) from Testing

14. Scroll to and select 014 Boron from menu.At the end of the Reagent Blank 30

15. Insert the tube into meter chamber, close lid and select Scan Blank.

16. Remove the tube from colorimeter.

17. At the end of the Sample Water 30 minute waiting period, remove Sample Water

18. Insert tube into meter chamber, close lid and select Scan Sample. Record result in

19. Press to turn colorimeter oﬀ or press the

ENTER

to select Testing Menu.

Menu.

minute waiting period, remove Reagent Blank tube from meter chamber. Invert several times to mix.

tube from Dark Storage Chamber. Invert several times to mix.

ppm boron.

EXIT

to exit to a previous menu

or make another menu selection.

Test Procedures

Smart3 Test Procedures 09.16 BORON

Test Procedures

BORON Smart3 Test Procedures 09.16

BROMINE

LIQUID DPD METHOD · CODE 4859

QUANTITY CONTENTS CODE

30 mL DPD 1A Free Chlorine Reagent P-6740-G

30 mL *DPD 1B Free Chlorine Reagent *P-6741-G

30 mL DPD 3 Total Chlorine Reagent P-6743-G

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Like chlorine,bromine is an eﬀective germicidal agent employed in drinking water treatment, pool and spa water sanitation, food service sanitation, and other public health applications.

APPLICATION: Drinking, surface, and saline waters; swimming pool water;

domestic and industrial waters and wastes.

RANGE: 0.00–9.00 Bromine

MDL: 0.10 ppm

METHOD: In buﬀered sample bromine reacts with diethyl-p-phenylene

diamine (DPD) to produce a pink-red color in proportion to the concentration of bromine present.

SAMPLE HANDLING & PRESERVATION:

INTERFERENCE: The only interfering substance likely to be encountered in

Bromine in aqueous solutions is not stable, and the bromine content of samples or solutions, particularly weak solutions, will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of bromine present in such solutions. For best results start analysis immediately after sampling. Samples to be analyzed for bromine cannot be preserved or stored.

water is oxidized manganese. The extent of this interference can be determined by treating a sample with sodium arsenite to destroy the bromine present so that the degree of interference can be estimated.

Test Procedures

Iodine and chlorine can also interfere, but these are not normally present unless they have been added as sanitizers.

Smart3 Test Procedures 09.16 BROMINE, Liquid, DPD

PROCEDURE

1. Press and hold until colorimeter turns on.

2. Press

3. Select All Tests (or another sequence containing 015 Bromine L DPD) from Testing

4. Scroll to and select 015 Bromine L DPD from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber, close lid and select Scan Blank.

7. Remove tube from colorimeter. Add 5 drops of DPD 1A Free Chlorine Reagent

8. Insert tube into chamber, close lid and select Scan Sample.

9. Press to turn colorimeter oﬀ or press the

NOTE: To test for bromine in the presence of chlorine, add 5 drops of Glycine Solution (6811-E) (sold separately) before adding the DPD Reagents.

ENTER

to select Testing Menu.

Menu.

(P-6740). Add 5 drops of *DPD 1B Free Chlorine Reagent (P-6741). Cap and mix. Solution will turn pink if bromine is present.

EXIT

to exit to a previous menu or

make another menu selection.

Test Procedures

BROMINE, Liquid, DPD Smart3 Test Procedures 09.16

BROMINE

DPD TABLET METHOD • CODE 3643-SC

QUANTITY CONTENTS CODE

100 DPD #1 IG Tablets 6903A-J

100 DPD #3 IG Tablets 6197A-J

15 mL Glycine Solution 6811-E

1 Tablet Crusher 0175

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Like chlorine, bromine is an eﬀective germicidal agent employed in drinking water treatment, pool and spa water sanitization, food service sanitation, and other public health applications.

Test Procedures

Smart3 Test Procedures 09.16 BROMINE, Tablet, DPD

APPLICATION: Drinking, surface, and saline waters; swimming pool water;

domestic and industrial waters and wastes.

RANGE: 0.00–9.00 Bromine

MDL: 0.10 ppm

METHOD: In buﬀered sample bromine reacts with diethyl-p-phenylene

diamine (DPD) to produce a pink-red color in proportion to the concentration of bromine present.

SAMPLE HANDLING & PRESERVATION:

Bromine in aqueous solutions is not stable, and the bromine content of samples or solutions, particularly weak solutions, will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of bromine present in such solutions. For best results start analysis immediately after sampling. Samples to be analyzed for bromine cannot be preserved or stored.

INTERFERENCE: The only interfering substance likely to be encountered in

water is oxidized manganese. The extent of this interference can be determined by treating a sample with sodium arsenite to destroy the bromine present so that the degree of interference can be estimated.

Iodine and chlorine can also interfere, but these are not normally present unless they have been added as sanitizers.

Test Procedures

BROMINE, Tablet, DPD Smart3 Test Procedures 09.16

PROCEDURE A: BROMINE (NO CHLORINE)

1. Press and hold until colorimeter turns on.

2. Press

ENTER

to select Testing Menu.

3. Select All Tests (or another sequence containing 016 Bromine T DPD) from Testing Menu.

4. Scroll to and select 016 Bromine T DPD from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber, close lid and select Scan Blank.

7. Remove tube from colorimeter. Add one DPD #1 IG Tablet (6903A). Cap tube and and shake for 10 seconds. Invert slowly 5 times. Solution will turn pink if bromine is present.

8. Insert tube into chamber, close lid and select Scan Sample.

9. Press to turn colorimeter oﬀ or press the

EXIT

to exit to a previous menu or

make another menu selection.

PROCEDURE B: BROMINE IN THE PRESENCE OF CHLORINE

1. Press and hold until colorimeter turns on.

2. Press

ENTER

to select Testing Menu.

3. Select All Tests (or another sequence containing 016 Bromine T DPD) from Testing Menu.

4. Scroll to and select 016 Bromine-LR from menu.

5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.

6. Insert tube into chamber close lid and select Scan Blank.

7. Remove blank from colorimeter. Add 5 drops of Glycine Solution (6811). Cap and mix.

8. Add one DPD#1 IG Tablet (6903A). Cap tube and shake for 10 seconds. Invert slowly 5 times. Solution will turn pink if bromine is present. Insert tube into chamber, close lid and select Scan Sample.

9. Press to turn colorimeter oﬀ or press the

EXIT

to exit to a previous menu or

make another menu selection.

Smart3 Test Procedures 01.18 BROMINE, Tablet, DPD

Test Procedures

PROCEDURE C: FREE AVAILABLE, TOTAL AVAILABLE & COMBINED CHLORINE IN THE PRESENCE OF BROMINE

1. Perform the test for Free and Combined Chlorine.

2. Perform the test for bromine in the presence of chlorine.

Calculations:

Residual Bromine (ppm) = Reading BR

Free Chlorine in the Presence of Bromine = Free Chlorine - 0.45 (Reading BR)

Total Chlorine in the Presence of Bromine = Total Chlorine - 0.45 (Reading BR)

Combined Chlorine in the Presence of Bromine = Total Chlorine - Free Chlorine

NOTE: Combined chlorine is not aﬀected by the presence of bromine, so the calculation is the same as when only chlorine is present.

Test Procedures

Smart3 Test Procedures 09.16 BROMINE, Tablet, DPD

BROMINE - UDV

DPD METHOD–UNIT DOSE VIALS · CODE 4311-J

QUANTITY CONTENTS CODE

100 Free Chlorine Unit Dose Vials, 20 pouches 4311-J

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com. Search for the four digit reagent code number listed on the reagent label, in the contents list or in the test procedures. Omit any letter that follows or precedes the four digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy, contact LaMotte by email, phone or fax.

Emergency information for all LaMotte reagents is available from Chem-Tel: (US, 1-800-255-3924) (International, call collect, 813-248-0585).

Equipment needed but not supplied:

STANDARD ACCESSORY PACKAGE · CODE 1961

1 Package of 3 Vials (empty) 0156

1 Syringe, 3 mL, plastic 1184

1 Foil Storage Bag 9467

Or:

ADVANCED ACCESSORY PACKAGE · CODE 1962

1 Pipettor, 3mL, w/tip 30529-BRAND

1 Cuvette Rack 31695

1 Package of 3 Vials (empty) 0156

1 Foil Storage Bag 9467

Like chlorine, bromine is an eﬀective germicidal agent employed in drinking water treatment, pool and spa water sanitization, food service sanitation, and other public health applications.

Smart3 Test Procedures 11.18 BROMINE, UDV

Test Procedures

APPLICATION: Drinking, surface, and saline waters; swimming pool water;

domestic and industrial waters and wastes.

RANGE: 0.0–22.0 ppm Bromine

MDL: 0.10 ppm

METHOD: In buﬀered sample bromine reacts with diethyl-p-phenylene

diamine (DPD) to produce a pink-red color in proportion to the concentration of bromine present.

SAMPLE HANDLING & PRESERVATION:

Bromine in aqueous solutions is not stable, and the bromine content of samples or solutions, particularly weak solutions, will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of bromine present in such solutions. For best results start analysis immediately after sampling. Samples to be analyzed for bromine cannot be preserved or stored.

INTERFERENCE: The only interfering substance likely to be encountered in

water is oxidized manganese. The extent of this interference can be determined by treating a sample with sodium arsenite to destroy the bromine present so that the degree of interference can be estimated.

Iodine and chlorine can also interfere, but these are not normally present unless they have been added as sanitizers.

Test Procedures

BROMINE, UDV Smart3 Test Procedures 11.18

Lamotte SMART3 Colorimeter User Manual

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Frequently Asked Questions

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