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PHOSPHONATE TEST KIT
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DIRECT READING TITRATOR METHOD
MODEL OP-DR · CODE 7634-DR
QUANTITY CONTENTS CODE
15 mL *Hydrochloric Acid, 1.0N *6130-E
30 mL Meta Cresol Purple Indicator 2202-G
15 mL Sodium Thiosulfate, 0.1N 6155-E
10 g Xylenol Orange Powder 6165-D
60 mL Thorium Nitrate Solution 6158PS-H
1 Test Tube, 10 mL, glass 0822
1 Test Tube, 5-10-15 mL, glass, w/cap 0778
1 Special Perforated Cap 0601
1 Spoon, 0.1 g 0699
1 Direct Reading Titrator, 0-20 Range 0378
1 Low-High pH Octet Comparator 7712
*WARNING: Reagents marked with a * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these
reagents see MSDS CD or www.lamotte.com. To obtain a printed copy,
contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code
number.
NOTE: Carefully read the instruction manual for the Direct Reading
Titrator before performing the titration procedure described below. The
Titrator is calibrated in terms of parts per million Phosphonate, and
each minor division on the Titrator scale equals 0.4 ppm.
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USE OF THE OCTET COM PARA TOR
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The Octet Comparator contains eight
permanent color standards. A test sample is
inserted into the openings in the top of the
comparator. The sample can then be compared
to four color standards at once, and the value
read off the comparator. For optimum color
comparison, the comparator should be
positioned between the operator and a light
source, so that the light enters through the
special light-diffusing screen in the back of the
comparator. Avoid viewing the comparator
against direct sunlight or an irregularly lighted
background.
pH AD JUST MENT
Prior to performing the actual test titration, the pH of the test sample
must be adjusted to approximately pH 2.6. The pH adjustment is made
by adding *Hydrochloric Acid to the test sample. The amount of
*Hydrochloric Acid to be added is determined as follows:
1. Fill the test tube (0822) to the 10 mL line with the sample water.
2. Add 14 drops of Meta Cresol Purple Indicator (2202). A color
somewhere between yellow and purple will develop, depending on
the pH of the sample.
3. Add *Hydrochloric Acid (6310), one drop at a time, mixing
between drops, counting the number of drops, and continually
comparing the color of the sample against the two pH color
standards in the Low-High pH Comparator (7712). When the
sample color is between these two standard colors, the sample has
been adjusted to the proper pH. The sample color must not be too
yellow or too red.
4. Record the number of drops of *Hydrochloric Acid used in Step C.
The same number of drops will be added to the sample in Step 3 of
the Test Procedure.
5. Discard this sample.
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PRO CE DURE
1. Fill the test tube (0778) to the 10 mL line with sample water.
2. Add 1 drop of Sodium Thiosulfate (6155).
3. Add the exact number of drops of *Hydrochloric Acid (6130)
recorded in Step D above.
4. Use the 0.1 g measuring spoon (0699) to add one level measure of
Xylenol Orange Powder (6165). Swirl to dissolve. The solution will
turn yellow.
5. Replace the regular cap on the bottle of Thorium Nitrate (6158PS)
with the special perforated dispenser cap (0601). Fill the Direct
Reading Titrator (0378) with this reagent. Insert the Titrator into
the center hole of the titration tube cap.
6. While gently swirling the titration tube, slowly press the plunger to
titrate until the solution changes from yellow to pink.
7. Read the test result directly from the scale where the large ring on
the Titrator meets the Titrator barrel.
8. Replace the regular cap on the Thorium Nitrate bottle for storage.
9. For most accurate results, a blank test should be run on a sample of
the water containing no Phosphonate. Any result from this blank
test should be subtracted from the result recorded in Step 7 above.
10. This test has been calibrated for Dequest 2006. When a different
compound is to be tested, the amount of Thorium Nitrate added
should be multiplied by a conversion factor to determine ppm
Phosphonate.
Phosphonates Compound Name Factor
Dequest 2000 AMP(NTP) 0.8
Dequest 2006 NaAMP 1.0
Dequest 2010 HEDP(A) 0.6
Belcor 575 0.5
11. If any other Phosphonate compound is used, the factor must be
determined experimentally using standard solutions of that
compound.
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LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 · Chestertown · Mary land · 21620 · USA
800-344-3100 · 410-778-3100 (Out side USA) · Fax 410-778-6394
Visit us on the web at www.lamotte.com
®
04 /09
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