Page 1
SMART3 Soil
Colorimeter
Operator’s
Manual
Firmware Version 2.10
1985-05-MN-V3
10/19
Warning! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision.
1985-05-MN-V3.indd
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 2
CONTENTS
GENERAL INFORMATION
Packaging & Delivery ............................................................................................................................ 5
General Precautions ........................................................................................................................... 5
Safety Precautions ..............................................................................................................................5
Limits of Liability .................................................................................................................................. 6
Warranty ................................................................................................................................................. 6
Register Your Meter .............................................................................................................................. 6
Specifi cations ...................................................................................................................................... 7
Statistical and Technical Defi nitions ............................................................................................... 9
Contents and Accessories ............................................................................................................... 10
EPA Compliance ..................................................................................................................................11
CE Compliance .................................................................................................................................... 11
IP 67 Certifi cation ...............................................................................................................................11
CHEMICAL TESTING
An Introduction to Colorimetric Analysis ..................................................................................... 12
Reagent Blank .................................................................................................................................... 13
Colorimeter Tubes & Chamber ....................................................................................................... 13
Meter Care............................................................................................................................................ 14
Sample Dilution & Volumetric Measurements ........................................................................... 14
Interferences ...................................................................................................................................... 15
Stray Light Interference .................................................................................................................. 15
OPERATION OF THE SMART3 COLORIMETER
Overview .............................................................................................................................................. 16
Components ....................................................................................................................................... 17
GENERAL OPERATING PROCEDURES
The Keypad ......................................................................................................................................... 18
Sample Holders .................................................................................................................................. 19
The Display & the Menus ................................................................................................................. 19
TESTING
Testing Menu ...................................................................................................................................... 21
General Testing Procedures ............................................................................................................ 22
Testing With LaMotte Pre-Programmed Tests .......................................................................... 22
Calibrating LaMotte Pre-Progammed Tests ................................................................................ 24
Measuring in the Absorbance Mode .............................................................................................. 28
EDITING MENU
Test Sequences....................................................................................................................................31
Editing a Sequence ........................................................................................................................... 32
Adding or Deleting a Test ................................................................................................................ 34
Creating a Soil Sequence Test .........................................................................................................38
Edit User Tests ................................................................................................................................... 45
Naming the Test ................................................................................................................................ 47
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 3
Selecting the Vial and Wavelength ............................................................................................... 49
Entering a Two Point Calibration ................................................................................................... 50
Entering a Multiple Point Calibration ..............................................................................................54
Selecting the Numerical Format of the Result ...........................................................................57
Selecting Units of Concentration ................................................................................................... 58
Setting the Clock ................................................................................................................................ 59
Logging Data ....................................................................................................................................... 60
Factory Setup ...................................................................................................................................... 61
Setting Power Save ............................................................................................................................ 61
Setting Backlight Time ......................................................................................................................62
Bluetooth Menu ...................................................................................................................................63
Selecting a Language .........................................................................................................................70
Looping Menus .....................................................................................................................................72
COMPUTER CONNECTION
Output ..................................................................................................................................................73
Computer Connection ....................................................................................................................... 73
Bluetooth Connection ........................................................................................................................73
BATTERY
Battery/AC Operation .........................................................................................................................74
Battery Replacement .........................................................................................................................75
MAINTENANCE
Cleaning ...............................................................................................................................................75
Repairs .................................................................................................................................................. 75
Meter Disposal .................................................................................................................................... 75
TROUBLESHOOTING
Error Messages ................................................................................................................................... 76
Troubleshooting Guide .......................................................................................................................77
Software Troubleshooting Guide .................................................................................................... 77
SMART3 COLORIMETER SOIL TEST PROCEDURES
APPENDIX
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 4
GENERAL INFORMATION
PACKAGING & DELIVERY
Experienced packaging personnel at LaMotte Company assure adequate protection
against normal hazards encountered in transportation of shipments. After the
product leaves the manufacturer, all responsibility for its safe delivery is assured
by the transportation company. Damage claims must be fi led immediately with the
transportation company to receive compensation for damaged goods.
Should it be necessary to return the instrument for repair or servicing, pack
instrument carefully in a suitable container with adequate packing material. A return
authorization number must be obtained from LaMotte Company by calling 1-800344-3100 or emailing tech@lamotte.com. Attach a letter with the authorization
number to the shipping carton which describes the kind of trouble experienced. This
valuable information will enable the service department to make the required repairs
more effi ciently.
GENERAL PRECAUTIONS
Before attempting to set up or operate this instrument it is important to read the
instruction manual. Failure to do so could result in personal injury or damage to the
equipment.
The Smart3 Colorimeter should not be stored or used in a wet or corrosive
environment. Care should be taken to prevent water or reagent chemicals from wet
colorimeter tubes from entering the colorimeter chamber.
NEVER PUT WET TUBES IN COLORIMETER.
SAFETY PRECAUTIONS
Read the labels on all LaMotte reagent containers prior to use. Some containers
include precautionary notices and fi rst aid information. Certain reagents are
considered potentially hazardous substances and are designated with a * in the
instruction manual. Safety Data Sheets (SDS) can be found at www.lamotte.com.
Read the SDS before using these reagents. Additional emergency information for
all LaMotte reagents is available 24 hours a day from the National Poison Control
Center 1-800-222-1222 or by contacting the 24 hour emergency line for ChemTel
1-800-255-3924 (USA, Canada, Puerto Rico); locations outside the North American
Continent 813-248-0585 (call collect). Be prepared to supply the name and four-digit
LaMotte code number found on the container label or at the top of the SDS or in the
contents list of the procedure. LaMotte reagents are registered with a computerized
poison control information system available to all local poison control centers.
Keep equipment and reagent chemicals out of the reach of young children.
Ensure that the protection provided by this equipment is not impaired. Do not intall or
use this equipment in a manner that is not indicated in this manual.
SMART3 Soil Colorimeter 9.18 5
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 5
LIMITS OF LIABILITY
Under no circumstances shall LaMotte Company be liable for loss of life, property,
profi ts, or other damages incurred through the use or misuse of its products.
WARRANTY
LaMotte Company warrants this instrument to be free of defects in parts and
workmanship for 2 years from the date of shipment. If it should become necessary to
return the instrument for service during or beyond the warranty period, contact our
Technical Service Department at 1-800-344-3100 or
tech@lamotte.com for a return authorization number or visit www.lamotte.com
for troubleshooting help. The sender is responsible for shipping charges, freight,
insurance and proper packaging to prevent damage in transit. This warranty does not
apply to defects resulting from action of the user such as misuse, improper wiring,
operation outside of specifi cation, improper maintenance or repair, or unauthorized
modifi cation. LaMotte Company specifi cally disclaims any implied warranties or
merchantability or fi tness for a specifi c purpose and will not be liable for any direct,
indirect, incidental or consequential damages. LaMotte Company’s total liability
is limited to repair or replacement of the product. The warranty set forth above is
inclusive and no other warranty, whether written or oral, is expressed or implied.
REGISTER YOUR METER
To register your meter with the LaMotte Service Department, go to
www.lamotte.com and choose SUPPORT on the top navigation bar.
6 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 6
SPECIFICATIONS
INSTRUMENT TYPE: Colorimeter
Readout 160 x 100 backlit LCD, 20 x 6 line graphical display
Wavelengths 428 nm, 525 nm, 568 nm, 635 nm
Wavelength Accuracy ±2% FS
Readable Resolution Determined by reagent system
Wavelength Bandwidth 10 nm typical
Photometric Range –2 to +2 AU
Photometric Precision ± 0.001 AU at 1.0 AU
Photometric Accuracy ±0.005 AU at 1.0 AU
Sample Chamber Accepts 25 mm diameter fl at-bottomed test tubes, 10
mm square cuvettes†, 16 mm COD test tubes
Light Sources 4 LEDs
Detectors 4 silicon photodiodes
Modes Pre-programmed tests, absorbance, %T
Pre-Programmed Tests YES, with automatic wavelength selection
User Defi ned Tests Up to 25 user tests can be input
Languages English, Spanish, French, Portuguese, Italian, Chinese,
Japanese
Temperature Operation: 0-50 °C; Storage: -40-60 °C
Operation Humidity
Range
USB Port Mini B
Power Requirements USB wall adapter, USB computer connection or lithium
Battery Charge Life: Approximately 380 tests with backlight on to
Electrical Rating Rated voltage (5V), Rated power of input current (1.0A) at
Data Logger 500 test results stored for download to a PC
Waterproof IP67 with USB port plug in place
0-90 % RH, non-condensing
ion rechargeable battery
1000 tests with backlight off . (Signal averaging disabled).
Battery Life: Approximately 500 charges.
mini-USB input port
SMART3 Soil Colorimeter 9.18 7
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 7
Certifi cations
BT Radio FCC ID:
T9J-RN42
Cert#
BQB
LISTED
CE Mark Safety
6514A-RN42
B014867-SPP and DUN
profi les
EN61610-1:2010
+Corrigendum 1:2011
EMC
EN 301 489-17 and
EN 301 489
US EMC FCC CFR 47, Part 15,
subpart B
Canada EMC CAN ICES-3(A)/NMB-3(A)
AU/NZ EMC AS/NZS CISPR 22
Dimensions (LxWxH) 3.5 x 7.5 x 2.5 inches, 8.84 x 19.05 x 6.35 cm
Weight 13 oz, 362 g (meter only)
†525 nm and 568 nm only
8 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
3.17
Page 8
STATISTICAL & TECHNICAL DEFINITIONS RELATED TO
PRODUCT SPECIFICATIONS
Method Detection Limit (MDL): “The method detection limit (MDL) is defi ned as the
minimum concentration of a substance that can be measured and reported with 99%
confi dence that the analyte concentration is greater than zero and is determined
from analysis of a sample in a given matrix containing the analyte.”1 Note that, “As Dr.
William Horwitz once stated, ‘In almost all cases when dealing with a limit of detection
or limit of determination, the primary purpose of determining that limit is to stay away
from it.’”
Accuracy: Accuracy is the nearness of a measurement to the accepted or true
value.3 The accuracy can be expressed as a range, about the true value, in which a
measurement occurs (i.e. ±0.5 ppm). It can also be expressed as the % recovery of a
known amount of analyte in a determination of the analyte (i.e. 103.5 %).
Resolution: Resolution is the smallest discernible diff erence between any two
measurements that can be made.4 For meters this is usually how many decimal places
are displayed. (i.e. 0.01). Note that the resolution many change with concentration
or range. In some cases the resolution may be less than the smallest interval, if it is
possible to make a reading that falls between calibration marks. A word of caution,
that resolution has very little relationship to accuracy or precision. The resolution
will always be less than the accuracy or precision but it is not a statistical measure
of how well a method of analysis works. The resolution can be very, very good and
the accuracy and precision can be very bad! This is not a useful measure of the
performance of a test method.
Repeatability: Repeatability is the within-run precision.5 A run is a single data set,
from set up to clean up. Generally, one run occurs on one day. However, for meter
calibrations, a single calibration is considered a single run or data set, even though it
may take 2 or 3 days.
Reproducibility: Reproducibility is the between-run precision.
2
6
Detection Limit (DL): The detection limit (DL) for the 2020we/wi
is defi ned as the minimum value or concentration that can be determined by the
meter, which is greater than zero, independent of matrix, glassware, and other sample
handling sources of error. It is the detection limit for the optical system of the meter.
1
CFR 40, part 136, appendix B
2
Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta,
American Laboratory, Sept 2003, P. 31.
3
Skoog, D.A., West, D. M., Fundamental of Analytical Chemistry, 2nd ed., Holt Rinehart
and Winston, Inc, 1969, p. 26.
4
Statistics in Analytical Chemistry: Part 7 – A Review, D. Coleman and L Vanatta,
American Laboratory, Sept 2003, P. 34.
5
Jeff ery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative
Chemical Analysis, 5th ed., Longman Scientifi c & Technical, 1989, p. 130.
SMART3 Soil Colorimeter 9.18 9
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 9
6
Jeff ery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative
Chemical Analysis, 5th ed., Longman Scientifi c & Technical, 1989, p. 130
CONTENTS AND ACCESSORIES
Contents
Smart3 Colorimeter
Test Tubes, with Caps Code 0290
COD/UDV Adapter Code 1724
USB Wall Adapter Code 1721
USB Cable Code 1720-01
Smart3 Colorimeter Quick Start Guide
Smart3 Colorimeter Manual
Accessories
Test Tubes, with Caps Code 0290-6
Replacement Chamber Code 3-0038
USB Cable Code 1720-01
USB Wall Adapter Code 1721
COD/UDV Adapter Code 1724
Bluetooth Mobile Printer Code 3-0066
Car Charger Code 5-0132
Small Field Carrying Case
(37.5 27.5 x 13.75 cm)
Large Field Carrying Case
(45 x 32.5 x 20 cm)
Code 1910-GCS150
Code 1910-GCS440
WARNING: Only use the USB Cable (1720-01) that is supplied with the kit.
Make no substitutions.
10 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 10
EPA COMPLIANCE
The Smart3 Colorimeter is an EPA-Accepted instrument. EPA-Accepted means
that the instrument meets the requirements for instrumentation as found in test
procedures that are approved for the National Primary Drinking Water Regulations
(NPDWR) or National Pollutant Discharge Elimination System (NPDES) compliance
monitoring programs. EPA-Accepted instruments may be used with approved test
procedures without additional approval.
CE COMPLIANCE
The Smart3 Colorimeter has earned the European CE Mark of Compliance for
electromagnetic compatibility and safety. The Declaration of Conformity for the
Smart3 colorimeter is available at www.lamotte.com.
This device complies with Part 15 of the FCC rules. Operation is subject to the following
two conditions (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause
undesired operation.
Note: This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses, and can radiate
radio frequency energy and, if not installed and used in accordance with the instruction
manual, may cause harmful interference to radio communications. Operation of this
equipment in a residential area is likely to cause harmful interferences in which case
the user will be required to correct the interference at his own expense.
IP67 CERTIFICATION
The Smart3 meets IP67 standards for protection against dust and immersion only
when the USB port plug is in place. Documentation is available at www.lamotte.com.
SMART3 Soil Colorimeter 9.18 11
Find Quality Products Online at: sales@GlobalTestSupply.com
3.17
www.GlobalTestSupply.com
Page 11
AN INTRODUCTION TO COLORIMETRIC ANALYSIS
Most test substances in water are colorless and undetectable to the human eye. To
test for their presence we must fi nd a way to “see” them. The Smart3 Colorimeter
can be used to measure any test substance that is itself colored or can be reacted
to produce a color. In fact a simple defi nition of colorimetry is “the measurement of
color” and a colorimetric method is “any technique used to evaluate an unknown color
in reference to known colors”. In a colorimetric chemical test the intensity of the color
from the reaction must be proportional to the concentration of the substance being
tested. Some reactions have limitations or variances inherent to them that may give
misleading results. Many such interferences are discussed with each particular test
instruction. In the most basic colorimetric method the reacted test sample is visually
compared to a known color standard. However, accurate and reproducible results are
limited by the eyesight of the analyst, inconsistencies in the light sources, and the
fading of color standards.
To avoid these sources of error, a colorimeter can be used to photoelectrically
measure the amount of colored light absorbed by a colored sample in reference to a
colorless sample (blank).
White light is made up of many diff erent colors or wavelengths of light. A colored
sample typically absorbs only one color or one band of wavelengths from the white
light. Only a small diff erence would be measured between white light before it passes
through a colored sample versus after it passes through a colored sample. The reason
for this is that the one color absorbed by the sample is only a small portion of the total
amount of light passing through the sample. However, if we could select only that one
color or band of wavelengths of light to which the test sample is most sensitive, we
would see a large diff erence between the light before it passes through the sample
and after it passes through the sample.
The Smart3 Colorimeter passes one of four colored light beams through one of four
optical fi lters which transmits only one particular color or band of wavelengths of light
to the photodectector where it is measured. The diff erence in the amount of colored
light transmitted by a colored sample is a measurement of the amount of colored
light absorbed by the sample. In most colorimetric tests the amount of colored light
absorbed is directly proportional to the concentration of the test factor producing the
color and the path length through the sample. However, for some tests the amount of
colored light absorbed is inversely proportional to the concentration.
The choice of the correct wavelength for testing is important. It is interesting to
note that the wavelength that gives the most sensitivity (lower detection limit) for a
test factor is the complementary color of the test sample. For example the NitrateNitrogen test produces a pink color proportional to the nitrate-nitrogen concentration
in the sample (the greater the nitrate-nitrogen concentration, the darker the pink
color). A wavelength in the green region should be selected to analyze this sample
since a pinkish-red solution absorbs mostly green light.
12 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 12
REAGENT BLANK
Some tests will provide greater accuracy if a reagent blank is determined to
compensate for any color or turbidity resulting from the reagents themselves.
A reagent blank is performed by running the test procedure on demineralized or
deionized water. Use sample water to Scan Blank. Insert the reacted reagent blank
in the colorimeter chamber and select Scan Sample. Note result of reagent blank.
Perform the tests on the sample water as described. Subtract results of reagent blank
from all subsequent test results. NOTE: Some tests require a reagent blank to be used
to Scan Blank.
COLORIMETER TUBES AND CHAMBER
The colorimeter uses one type of tube (Code 0290) for all test factors.
The handling of the tubes is of utmost importance. Tubes must be clean and free from
lint, fi ngerprints, dried spills and signifi cant scratches, especially the central zone
between the bottom and the sample line.
Scratches, fi ngerprints and water droplets on the tube can cause stray light
interference leading to inaccurate results. Tubes that have been scratched in the light
zone through excessive use should be discarded and replaced with new ones.
Tubes should always be washed on the inside and outside with mild detergent prior to
use to remove dirt or fi ngerprints. The tubes should be allowed to air-dry in an inverted
position to prevent dust from entering the tubes. Dry tubes should be stored with the
caps on to prevent contamination.
After a tube has been fi lled and capped, it should be held by the cap and the outside
surface should be wiped with a clean, lint-free absorbent cloth until it is dry and
smudge-free. Handling the tube only by the cap will avoid problems from fi ngerprints.
Always set the clean tube aside on a clean surface that will not contaminate the tube.
It is imperative that the tubes and light chamber be clean and dry. The outside of the
tubes should be dried with a clean, lint-free cloth or disposable wipe before they are
placed in the meter chamber.
Tubes should be emptied and cleaned as soon as possible after reading a sample to
prevent deposition of particulates on the inside of the tubes.
Variability in the geometry of the glassware and technique is the predominate cause of
variability in results. Slight variations in wall thickness and the diameter of the tubes
may lead to slight variations in the test results. To eliminate this error the tubes should
be placed in the chamber with the same orientation each time.
Chambers which have been scratched through excessive use should be discarded and
replaced with a new one.
SMART3 Soil Colorimeter 9.18 13
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 13
METER CARE
The optical system of the Smart3 must be kept clean and dry for optimal performance.
Dry the colorimeter tubes before placing them in the chamber to avoid introducing
moisture. For best results store the instrument in a area that is dry and free from
aggressive chemical vapors.
CALIBRATION
As with all pre-calibrated meters, it is highly recommended, even if not required by
regulations, that the user periodically verify the performance of the meter by running
standards with a predetermined concentration. Results outside of specifi cation are
an indication that the meter needs to be adjusted. This can be done following the user
calibration described on page 29. If the user calibration fails to properly adjust the
meter then the meter should be returned to LaMotte Company for recalibration. (See
page 74).
SAMPLE DILUTION TECHNIQUES & VOLUMETRIC
MEASUREMENTS
If a test result using the Smart3 Colorimeter gives an over range message then the the
sample must be diluted. The test should be repeated on the diluted sample to obtain
a reading which is in the concentration range for the test. (Note: This is not true for
colorimetric determination of pH.)
Example:
Measure 5 mL of the water sample into a graduated cylinder. Add demineralized
water until the cylinder is fi lled to the 10 mL line. The sample has been diluted by
one-half, and the dilution factor is therefore 2. Perform the test procedure, then
multiply the resulting concentration by 2 to obtain the test result.
The following table gives quick reference guidelines on dilutions of various proportions.
All dilutions are based on a 10 mL volume, so several dilutions will require small
volumes of the water sample. Graduated pipets should be used for all dilutions.
Deionized Water to
Size of Sample
10 mL 0 mL 1
5 mL 5 mL 2
2.5 mL 7.5 mL 4
1 mL 9 mL 10
0.5 mL 9.5 mL 20
If volumetric glassware is not available, dilutions can be made with the colorimeter
tube. Fill the tube to the 10 mL line with the sample then transfer it to another
container. Add 10 mL volumes of demineralized water to the container and mix.
Transfer back 10 mL of the diluted sample to the tube and follow the test procedure.
Continue diluting and testing until a reading, which is in the concentration range for
14 SMART3 Soil Colorimeter 9.18
Bring Volume to 10 mL Multiplication Factor
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 14
the test, is obtained. Be sure to multiply the concentration found by the dilution factor
(the number of total 10 mL volumes used).
Example:
10 mL of sample is diluted with three 10 mL volumes of demineralized water;
the dilution factor is four.
INTERFERENCES
LaMotte reagent systems are designed to minimize most common interferences. Each
individual test instruction discusses interferences unique to that test. Be aware of
possible interferences in the water being tested.
The reagent systems also contain buff ers to adjust the water sample to the ideal pH
for the reaction. It is possible that the buff er capacity of the water sample may exceed
the buff er capacity of the reagent system and the ideal pH will not be obtained. If
this is suspected, measure the pH of a reacted distilled water reagent blank using a
pH meter. This is the ideal pH for the test. Measure the pH of a reacted water sample
using the pH meter. If the pH is signifi cantly diff erent from the ideal value, the pH of
the sample should be adjusted before testing.
Interferences due to high concentration of the substance being tested, can be
overcome by sample dilution (see page 14)
STRAY LIGHT INTERFERENCE
When scanning samples in 16 mm tubes, such as COD, the sample chamber lid can
not be closed. The COD adapter minimizes stray light. To further reduce stray light
interference, do not scan sample in direct sunlight.
SMART3 Soil Colorimeter 9.18 15
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 15
OPERATION OF THE SMART3 COLORIMETER
OVERVIEW
The Smart3 is a portable, microprocessor controlled, direct reading colorimeter. It has
a graphical liquid crystal display and 6 button keypad. These allow the user to select
options from the menu driven software, to directly read test results or to review stored
results of previous tests in the data logger. The menus can be displayed in seven
diff erent languages.
The test library consists of over 80 LaMotte tests and 25 “User Tests”. The LaMotte
tests are precalibrated for LaMotte reagent systems.The colorimeter displays the
result of these tests directly in units of concentration. The 25 “User Tests” may be
used to enter additional calibrations. All of these tests may be arranged in any of 3
sequences. These sequences can be modifi ed a limitless number of times to meet
changing testing needs.
The optics feature 4 diff erent colored LEDs. Each LED has a corresponding silicon
photoiode with an integrated interference fi lter. The interference fi lters select a narrow
band of light from the corresponding LED for the colorimetric measurements. The
microporcessor automatically selects the correct LED/photodiode combination for the
test.
A USB wall adapter, USB computer connection or lithium battery powers the Smart3.
Bluetooth wireless technology allows communication between the colorimeter and the
Bluetooth Mobile Printer (Code 3-0066 only).
16 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 16
COMPONENTS
Figure 1 shows a diagram of the Smart3 Colorimeter and its components.
Top View
SMART3
ENTER
Bottom View
Serial
Number
COLORIMETER
EXIT
Lid
Side Views
USB Port
with plug
Figure 1
SMART3 Soil Colorimeter 9.18 17
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 17
GENERAL OPERATING PROCEDURES
The operation of the Smart3 Colorimeter is controlled by a microprocessor. The
microprocessor is programmed with menu driven software. A menu is a list of choices.
This allows a selection of various tasks for the colorimeter to perform, such as, scan
blank, scan sample, and edit test sequences. The keypad is used to make menu
selections which are viewed in the display. There are two selections accessible from the
Main Menu: Testing Menu and Editing Menu.
THE KEYPAD
The keypad has 6 buttons which are used to perform specifi c tasks.
This button will scroll up through a list of menu selections or
go to the next character on a character selection line.
ENTER
EXIT
The button is used to select choices in a menu viewed in the
display and to add the selected character to the name and
advance one character.
This button controls the backlight on the display.
This button will scroll down through a list of menu selections
or go to the previous character selection line.
This button exits to the previous menu on the name edit
screen, the name will not be saved.
This button turns the meter on or off .
ENTER
EXIT
18 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 18
SAMPLE HOLDERS
The sample chamber is designed for 25 mm round tubes. An adapter to hold 16
mm COD tubes and 10 mm square UDV cuvettes is included. 10 cm cuvettes can be
scanned only at 525 nm and 568 nm. The light path is blocked at 428 nm and 635 nm
with the use of the adapter. COD tubes can be scanned at all wavelengths.
Position the COD/UDV Adapter (Code 1724) so that the
notches in the adapter fi t around the posts on the chamber.
Turn the adapter counterclockwise until the arrows are at
the top and bottom of the chamber and the adapter is locked
into place. Turn the adapter clockwise to unlock the adapter
and remove it from the chamber.
THE DISPLAY & THE MENUS
The display allows menu selections to be viewed and selected. These selections
instruct the SMART3 to perform specifi c tasks. The menus are viewed in the display
using two general formats that are followed from one menu to the next. Each menu is
a list of choices or selections.
The display has a header line at the top and a footer line at the bottom. The header
displays the title of the current menu. The footer line displays the time and the date,
the data logger status, the bluetooth/printer status and the battery status. The menu
selection window is in the middle of the display between the header and the footer.
The menu selection window displays information in two general formats. In the fi rst
format only menu selections are displayed. Up to 4 lines of menu selections may be
displayed. If more selections are available they can be viewed by pressing the arrow
buttons to scroll the other menu selections into the menu selection
window. Think of the menu selections as a vertical list in the display that moves up
or down each time an arrow button is pressed. Some menus in the
Smart3 are looping menus. The top and bottom menu choices are connected in a loop.
Scrolling down past the bottom of the menu will lead to the top of the menu. Scrolling
up past the top of the menu will lead to the bottom of the menu.
Header Menu Title
Main Window Selection First Choice
Second Choice
Third Choice
Another
Footer
SMART3 Soil Colorimeter 9.18 19
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
12:00:00 001/500 P
And Another
And So On
Page 19
A light bar will indicate the menu choice. As the menu is scrolled through, the light bar
will highlight diff erent menu choices. Pressing the
choice that is indicated by the light bar.
In the second format the menu choice window takes advantage of the graphical
capabilities of the display. Large format graphic information, such as test results or
error messages or the LaMotte logo is displayed. The top two lines of the display are
used to display information in a large, easy to read format. The menus work in the
same way as previously described but two lines of the menu are visible at the bottom
of the display.
Header Menu Title
Message or Result Window
ENTER
button will select the menu
Result or
Message
Another
Main Window Selection And Another
Footer
12:00:00 001/500
And So On
Last Choice
As described previously, the
menu and a return to the previous menu. This allows a rapid exit from an inner menu
to the main menu by repeatedly pushing the
will turn the Smart3 off .
The display may show the following messages:
Battery Status
More choices are available and can be viewed by scrolling
up and/or down through the display.
Header Identifi es the current menu and information on units and
reagent systems if applicable.
Footer In the data logging mode the number of the data point
is displayed and the total number of data points in the
memory will be shown. The footer also shows current time
and battery status
20 SMART3 Soil Colorimeter 9.18
EXIT
button allows an exit or escape from the current
EXIT
button. Pushing at any time
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 20
TESTING
TESTING MENU
The Testing Menu is used to run all LaMotte pre-programmed tests, User Tests and
Absorbance tests at one of four wavelengths. Testing from any of three sequences can
also be done.
1. Press and briefl y hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
2. Press
ENTER
to select Testing
Menu.
3. Press or to scroll
to desired option. All Tests
contains all of the available preprogrammed tests. The three
sequences have user selected
tests. Absorbance has %T/ABS
tests.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Testing Menu
All Tests Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
Testing Menu
All Tests Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
4. Press
Tests.
ENTER
to select All
002 Alkalinity UDV
All Tests
005 Aluminum
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 21
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 21
GENERAL TESTING PROCEDURES
The following is a step-by-step example of how to run tests from the Testing Menu.
These test procedures are designed to be used with LaMotte SMART Reagent Systems.
LaMotte Company continuously updates the list of pre-programmed tests as the
calibrations become available. Call LaMotte Technical Services at 1-800-344-3100
(410-778-3100 outside the USA) or email at tech@lamotte.com for a current list of
available calibrations.
TESTING WITH LaMOTTE PRE-PROGRAMMED TESTS
1. Press and briefl y hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
2. Press
ENTER
to select Testing
Menu.
3. Press
ENTER
to select All Tests
Menu.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Testing Menu
All Test Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
All Tests
002 Alkalinity UDV
005 Aluminum
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
4. Press or to scroll to
the desired test.
002 Alkalinity UDV
All Tests
005 Aluminum
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
22 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 22
5. Press
test.
ENTER
to select the
005 Aluminum
Scan Bank
Scan Sample
12:00:00 001/500
6. Insert the blank into the
chamber. Close the lid.Press
ENTER
to scan the blank. The
screen wil display Scan Blank
Blank Done for about 1 second
and then return to the test
menu.
7. Insert the reacted sample into
the chamber. Close the lid. Press
ENTER
to scan the sample. The
screen will display Scan Sample
Sample Done for about 1
second. The result will appear on
the screen.
8. To repeat the test, press
to scan the sample again. The
last blank scanned is used by
the colorimeter for repeated
scans. A diff erent blank can
be used by pressing or
to scroll to Scan Blank
and then scanning another
blank. Scroll with or
and make another
selection with
Absorbance of the last test can
be viewed by scrolling down and
choosing %T/Abs. Press
to escape to previous menus.
NOTE: The menus loop in this
screen so either or
will lead to the menu selection
needed.
ENTER
ENTER
. The %T or
EXIT
005 Aluminum
Scan Blank
Scan Sample
12:00:00 001/500
005 Aluminum
1.00 ppm
Scan Blank
Scan Sample
12:00:00 001/500
005 Aluminum
1.00 ppm
Scan Bank
Scan Sample
12:00:00 001/500
To Use the Print Test Function,
See the Bluetooth Menu Selection,
(page 63).
SMART3 Soil Colorimeter 9.18 23
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 23
CALIBRATING LaMOTTE PRE-PROGRAMMED TESTS
The LaMotte Pre-Programmed Tests have been pre-calibrated. Recalibration of
the pre-programmed tests by the user is not possible. However, a procedure to
standardize the calibration can be performed to obtain the most accurate readings or
to meet regulatory requirements.
The LaMotte Pre-Programmed tests are standardized with one standard solution. To
standardize over the full range of the test, the concentration of the standard should
be chosen from the high end of the range. Alternatively, if samples do not cover the full
range of the test, a standard should be chosen that is close to the concentration of
the samples.
For the SMART3 Soil colorimeter, the standard should be used instead of the soil
extract and prepared in distilled or deionized water for the range of the reagent
system before the multiplication factor has been applied to the reading on the display.
The following standards are recommended to standardize over the full range of the
tests:
Ammonia Nitrogen 3.00 ppm Ammonia Nitrogen
Copper 4.00 ppm Copper
Iron 4.00 ppm Iron
Manganese 11.00 ppm Manganese
Nitrate Nitrogen 2.00 ppm Nitrate Nitrogen
Nitrite Nitrogen 0.60 ppm Nitrite Nitrogen
Phosphorus 2.00 ppm Phosphate
Potassium 7.00 ppm Potassium
Sulfur 75 ppm Sulfate
Zinc 2.00 ppm Zinc
In the example below, the Aluminum calibration will be standardized.
Prepare a standard solution to be tested. In this example, 0.30 ppm aluminum.
24 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 24
1. Press and briefl y hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
2. Press
ENTER
to select Testing
Menu.
3. Press
ENTER
to select All Tests
Menu.
4. Press or to scroll to
the desired test factor.
Testing Menu
All Test Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
All Tests
002 Alkalinity UDV
005 Aluminum
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
All Tests
002 Alkalinity UDV
005 Aluminum
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
5. Press
ENTER
to select the
005 Aluminum
test.
Scan Blank
Scan Sample
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 25
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 25
6. Follow the test procedure in the
manual to test the prepared
standard. Insert the blank into
the chamber. Close the lid. Press
ENTER
to scan the blank. The
screen will display Scan Blank
Blank Done for about 1 second
and then return to the Test
Menu.
005 Aluminum
Scan Blank
Scan Sample
12:00:00 001/500
7. Insert the reacted standard
solution into the chamber. Close
the lid. Press
sample. The screen will display
Scan Sample Sample Done for
about 1 second. The result will
appear on the screen.
8. The displayed result can now be
standardized. Press or
to scroll to Calibrate.
9. Press
ENTER
A reverse font (light background
with dark characters) will appear
to indicate that the reading can
be adjusted.
ENTER
to scan the
to select Calibrate.
005 Aluminum
0.28 ppm
Scan Blank
Scan Sample
12:00:00 001/500
005 Aluminum
0.28 ppm
%T/Abs
Calibrate
12:00:00 001/500
005 Aluminum
0.28 ppm
, =Edit, ENTER=Save
+ENTER=Default
12:00:00 001/500
26 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 26
10. Press or to
adjust the value shown to the
concentration of the prepared
standard, 0.30 in this example.
NOTE: A maximum adjustment
of 25% is possible.
005 Aluminum
0.30 ppm
, =Edit, ENTER=Save
+ENTER=Default
12:00:00 001/500
Testing
11. Press
ENTER
to save the value.
To leave the Calibration
procedure without saving the
adjustment, press
Press and
time to return to the default
value.
The calibration has now been
standardized and can be used
for testing. Scroll to Scan Blank
and begin testing.
EXIT
ENTER
.
at any
005 Aluminum
0.30 ppm
%T/Abs
Calibrate
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 27
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 27
MEASURING IN THE ABSORBANCE MODE
1. Press and briefl y hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
2. Press
ENTER
to select Testing
Menu.
3. Press or to scroll to
Absorbance.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Testing Menu
All Test Menu
Sequence 1
Sequence 2
Sequence 3
12:00:00 001/500
Testing Menu
Sequence 1
Sequence 2
Sequence 3
Absorbance
12:00:00 001/500
4. Press
Absorbance.
ENTER
to select
101 Absorbance 428
Absorbance
102 Absorbance 525
103 Absorbance 568
104 Absorbance 635
12:00:00 001/500
5. Press or to scroll to
desired wavelength.
101 Absorbance 428
Absorbance
102 Absorbance 525
103 Absorbance 568
104 Absorbance 635
12:00:00 001/500
28 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 28
6. Press
wavelength.
ENTER
to select the
102 Absorbance 525
Testing
Scan Blank
Scan Sample
12:00:00 001/500
7. Insert the blank. Close the lid.
8. Insert the reacted sample. Press
ENTER
Press
The screen wil display Scan
Blank Blank Done for about
1 second and return to the
Absorbance menu.
ENTER
screen will display Scan Sample
Sample Done for about 1
second. The result will appear on
the screen.
to scan the blank.
to scan the sample. The
102 Absorbance 525
Scan Blank
Scan Sample
12:00:00 001/500
102 Absorbance 525
0.425
Scan Sample
Next Test
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 29
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 29
9. To repeat the test, scroll to Scan
ENTER
EXIT
ENTER
to
. The %T or
to escape
Sample and press
scan the sample again. The last
blank scanned is used by the
colorimeter for repeated scans.
A diff erent blank can be used by
Testing
pressing or to scroll
to Scan Blank and then scanning
another blank. Scroll with
or and make another
selection with
Absorbance of the last test can
be viewed by choosing
%T/Abs. Press
to previous menus.
NOTE: The menus loop in this
screen so either or
will lead to the menu selection
needed.
NOTE: The calibrate function
does not work in the Absorbance
mode.
0.425
Scan Sample
Next Test
12:00:00 001/500
Previous Test
%T/Abs
Calibrate
Print Test
102 Absorbance 525
EDITING MENU
The Editing Menu allows the user to edit sequences, edit user tests, set the clock, edit
the logging function, access factory setting, set the power saving function, set the
backlight time, and select a language.
The default factory settings are:
Date Format MM-DD-YYYY
Logging Enabled
Power Save 5 minutes
Backlight 10 seconds
Language English
30 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 30
TEST SEQUENCES
Sequence 1, Sequence 2, And Sequence 3 are alterable sequences. They may be edited
using the Editing Menu. Any of the LaMotte pre-programmed tests or User Tests may
be placed in these sequences in whatever testing order that is preferred. There are
three intial sequences in the Smart3 that can be customized by adding or deleting
tests:
Sequence 1 Sequence 2 Sequence 3
025 Chlorine T DPD 005 Aluminum 006 Ammonia-N LF
080 Phosphate HR 025 Chlorine T DPD 036 Cu Thiocarbamate
010 Benz
075 pH UDV 060 Manganese LR 068 Nitrite-N LR
12:00:00 001/500 12:00:00 001/500 12:00:00 001/500
063 Molybdenum HR 065 Nitrate-N LR 075 pH PR
086 Silica HR 068 Nitrite-N LR 081 Phosphate LR
036 Cu Thicarbamate 079 Phenol 087 Silica LR
055 Iron Bipyridyl 081 Phosphate LR
These alterable sequences allow a series of tests to be setup that are run frequently.
The order of the individual tests in the sequence can be determined by the user. After
running a test, press
pattern until the entire sequence has been completed.
otriazole 056 Iron Phenanthro 065 Nitrate-N LR
090 Sulfi de LR
ENTER
to select the next test in the sequence. Continue this
Testing
All Tests is a fi xed sequence containing the LaMotte pre-programmed tests, User
Tests, and Absorbance tests. Modifi cation of the alterable sequences is accomplished
through the Editing Menu. This menu is explained in greater detail in Editing Menu
(page 36).
Pressing
EXIT
while in a sequence menu will escape back to the Testing Menu.
Pressing the at any time will turn the colorimeter off .
NOTE: A sequence must contain at least one test.
SMART3 Soil Colorimeter 9.18 31
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 31
EDITING A SEQUENCE
The Edit Sequence menu allows three alterable test sequences (Sequence 1, Sequence
2, Sequence 3) to be edited.
1. Press and briefl y hold to
Testing
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
2. Press or to scroll to
the Editing Menu.
3. Press
ENTER
to select Editing
Menu.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
4. Press
Sequences.
ENTER
to select Edit
Edit Sequence 1
Edit Sequences
Edit Sequence 2
dit Sequence 3
E
12:00:00 001/500
32 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 32
5. Press or to scroll to
the desired sequence.
Edit Sequences
Edit Sequence 1
Edit Sequence 2
Edit Sequence 3
12:00:00 001/500
Testing
6. Press
ENTER
sequence to be edited.
to select the
EDIT SEQUENCE 2
005 Aluminum
025 Chlorine T DPD
056 Iron Phenanthro
060 Manganese LR
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 33
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 33
ADDING OR DELETING A TEST
There are three ways to alter a sequence: Insert Before, Insert After, and Delete. Insert
Before adds a new test to the sequence before the selected test. Insert After adds a
new test to the sequence after the selected test. Delete is used to remove an existing
test from a sequence.
Testing
ADDING A TEST
Below is a step-by-step example of how to add a test to SEQUENCE 2 starting from the
EDIT SEQUENCE 2 menu.
1. To add a test before or after an
existing test, press or
to scroll to the existing
test.
2. Press
ENTER
to select the
existing test.
3. Press or to scroll to
Insert Before or Insert After.
EDIT SEQUENCE 2
005 Aluminum
025 Chlorine T DPD
056 Iron Phenanthro
060 Manganese LR
12:00:00 001/500
Add or Delete
Insert Before
Insert After
e
Delet
12:00:00 001/500
Add or Delete
Insert Before
Insert After
e
Delet
12:00:00 001/500
4. Press
ENTER
to select the option,
Insert Before, in this example.
The All Test Menu will appear.
002 Alkalinity UDV
005 Aluminum
All Tests
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
34 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 34
5. Press or to scroll to
the test that will be added to the
sequence. In this example, 006
Ammonia-N LRS.
All Tests
002 Alkalinity UDV
005 Aluminum
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
Testing
6. Press
ENTER
to select the test.
The sequence will appear in the
Edit Sequence menu and the
new test will be added to the
sequence. All changes in the
sequence will be automatically
saved.
7. Press
EXIT
to exit the Edit
Sequence menu and return
to the Editing Sequences
Menu.
8. Press
ENTER
to select Edit
Sequences to continue editing
the sequences or press
twice to return to the Main
Menu.
EXIT
EDIT SEQUENCE 2
005 Aluminum
025 Chlorine T DPD
006 Ammonia-N LF
056 Iron Phenanthro
12:00:00 001/500
Edit Sequences
Edit Sequence 1
Edit Sequence 2
Edit Sequence 3
12:00:00 001/500
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 35
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 35
DELETING A TEST
Below is a step-by-step example of how to delete a test in SEQUENCE 2 starting from
the EDIT SEQUENCE 2 menu.
1. To delete a test, press or
Testing
to scroll to the test in the
sequence.
2. Press
ENTER
to select the
test.
3. Press or to scroll to
Delete.
EDIT SEQUENCE 2
005 Aluminum
025 Chlorine T DPD
006 Ammonia-N LF
056 Iron Phenathro
12:00:00 001/500
Add or Delete
Insert Before
Insert After
e
Delet
12:00:00 001/500
Add or Delete
Insert Before
Insert Aft
er
Delete
12:00:00 001/500
4. Press
36 SMART3 Soil Colorimeter 9.18
ENTER
to select Delete.
The sequence will appear in
the Edit Sequences menu and
the selected test will have been
deleted. All changes to the
sequence will automatically
have been saved.
005 Aluminum
025 Chlorine T DPD
056 Iron Phenanthro
060 Manganese LR
12:00:00 001/500
EDIT SEQUENCE 2
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 36
5. Press
EXIT
to exit the Edit
Sequence menu and return to
the Edit Sequences Menu.
Edit Sequences
Edit Sequence 1
Edit Sequences 2
Edit Sequences 3
12:00:00 001/500
Testing
6. Press
EXIT
to exit the Edit
Sequences menu and return to
the Editing Menu.
7. Press
ENTER
to select Edit
Sequences to continue editing
the sequences or press
return to the Main Menu.
EXIT
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
Main Menu
Testing Menu
to
Editing Menu
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 37
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 37
CREATING A SOIL TEST SEQUENCE
To create a soil testing sequence specifi cally for the reagent systems in this SMART3
Soil Manual follow the step by step procedure below. The test sequence currently in
the meter will be cleared and then the soil tests will be added to Sequence 1. Tests in
the soil sequence will be arranged in the order in which they appear in the manual.
008 Ammonia-N HR
036 Cu Thiocarbamate
055 Iron Bipyridyl
059 Manganese HR
065 Nitrate-N LR
068 Nitrite-N LR
081 Phosphate LR
085 Potassium
089 Sulfate HR
097 Zinc LR
Editing/Set Up
1. Press and briefl y hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
will appear.
2. Press or to scroll to
the Editing Menu.
3. Press
ENTER
to select Editing
Menu.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
38 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 38
4. Press
Sequences.
ENTER
to select Edit
Edit Sequences
Edit Sequence 1
Edit Sequence 2
dit Sequence 3
E
12:00:00 001/500
5. Press or to scroll
to the desired sequence. In this
exmple, Edit Sequence 1.
6. Press
ENTER
to select the
sequence to be edited.
7. Press
ENTER
to select the test to
be edited. In this example, 025
Chlorine T DPD.
Edit Sequences
Edit Sequence 1
Edit Sequence 2
dit Sequence 3
E
12:00:00 001/500
Edit Sequence 1
025 Chlorine T DPD
080 Phosphate HR
010 Benz
otriazole
075 pH PR
12:00:00 001/500
Add or Delete
Insert Before
Insert After
e
Delet
Editing/Set Up
12:00:00 001/500
8. Press or to scroll to
Delete.
Insert Before
Insert Aft
Add or Delete
er
Delete
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 39
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 39
9. Press
ENTER
to select Delete.
Edit Sequence 1
080 Phosphate HR
010 Benzotriazole
075 pH UD
V
063 Molybdenum HR
12:00:00 001/500
10. Press or and
to select the next test to be
edited. In this example, 080
Phosphate HR.
Editing/Set Up
11. Press or to scroll to
Delete.
12. Press
ENTER
to select Delete.
Repeat the procedure to delete
the tests in the series except
for 055 Iron Bipyridal which will
remain. Tests will be added to
the sequence.
NOTE: It is not possible to empty
a test sequence completely. One
test will remain. To remove the
remaining test, add a new test
and then delete the unwanted
test. In creating the soil
sequence, the last test—055
Iron Biryridal—will be included
in the sequence and will not be
deleted.
ENTER
Add or Delete
Insert Before
Insert After
e
Delet
12:00:00 001/500
Add or Delete
Insert Before
Insert Aft
er
Delete
12:00:00 001/500
Edit Sequence 1
055 Iron Bipyridyl
12:00:00 001/500
40 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 40
13. Press
ENTER
tests to the sequence.
to begin adding
Add or Delete
Insert Before
Insert After
e
Delet
12:00:00 001/500
14. Press
ENTER
to select Insert
Before.
15. Press or to scroll to
the fi rst test to be added to the
sequence. In this example, 036
Cu Thiocarbamate because the
tests will be added in the order
that they appear in the manual.
16. Press
ENTER
to select the test to
be added.
All Tests
002 Aluminum UDV
005 Alumnium
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
Add or Delete
003 Color
034 Cu BCA
T
035 Cu Cuprizone
036 Cu Thiocarbamate
12:00:00 001/500
Edit Sequence 1
036 Cu Thiocarbamate
055 Iron Bipyridal
Editing/Set Up
12:00:00 001/500
17. Press
Thiocarbamate.
ENTER
to select 036 Cu
036 Cu Thiocarbamate
Edit Sequence 1
055 Iron Bipyridal
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 41
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 41
18. Press or to scroll to
Insert Before.
Add or Delete
Insert Before
Insert After
e
Delet
12:00:00 001/500
19. Press
ENTER
to select Insert
Before. Press or
to scroll to the next test to be
added. In this example, 008
Ammonia-N HR.
Editing/Set Up
20. Press
ENTER
to select 008
Ammonia-N HR test to be
added.
21. Press to scroll to the last
test in the sequence. In this
example, 055 Iron Bipyridal.
Add or Delete
006 Ammonia-N LRF
007 Ammonia-N LRS
008 Ammonia-N HR
009 Barium
12:00:00 001/500
Edit Sequence 1
008 Ammonia-N HR
036 Cu Thiocarbamate
055 Iron Bip
12:00:00 001/500
yridal
Edit Sequence 1
008 Ammonia-N HR
036 Cu Thiocarbamat
e
055 Iron Bipyridal
12:00:00 001/500
22. Press
ENTER
to select 055 Iron
Bipyridal. Press or
to scroll to Insert After.
Insert Before
Insert After
Add or Delete
Delete
12:00:00 001/500
42 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 42
23. Press
Before.
ENTER
to select Insert
All Tests
002 Aluminum UDV
005 Alumnium
006 Ammonia-N LRF
007 Ammonia-N LRS
12:00:00 001/500
24. Press
ENTER
to select the test to
be added.
25. Press to scroll to the last
test in the sequence. In this
example, 059 Manganese HR.
26. Press
ENTER
to 059 Manganese
HR. Press or to
scroll to Insert After.
Edit Sequence 1
008 Ammonia-N HR
036 Cu Thiocarbamat
e
055 Iron Bipyridal
059 Manganese HR
12:00:00 001/500
All Tests
008 Ammonia-N HR
036 Cu Thiocarbamat
e
055 Iron Bipyridal
059 Manganese HR
12:00:00 001/500
Add or Delete
Insert Before
Insert After
Delete
Editing/Set Up
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 43
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 43
27. Press
ENTER
to select Insert
After. Repeat the procedure to
add the remaining tests to the
sequence.
059 Manganese HR
065 Nitrate-N LR
068 Nitrate-N LR
081 Phosphate LR
085 Potassium
089 Sulfate HR
097 Zinc LR
All Tests
081 Phosphate LR
otassium
285 P
089 Sulfate LR
099 Zinc LR
12:00:00 001/500
28. Press
EXIT
Sequences Menu.
Editing/Set Up
29. Press
EXIT
Editing Menu.
30. Press
EXIT
Main Menu. Press or
to scroll to the Testing
Menu.
to escape the
to escape the
to escape to the
Editing Menu
Edit Sequence 1
Edit Sequence 2
dit Sequence 3
E
12:00:00 001/500
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
44 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 44
31. Press
ENTER
to select Testing
Menu. Press or to
scroll to Sequence 1 to begin
testing.
All Test Menu
Sequence 1
Testing Menu
Sequence 2
Sequence 3
12:00:00 001/500
EDIT USER TESTS
If a test other than the LaMotte programmed tests is performed regularly, a calibration
for it may be entered in one of the 25 User Tests. These tests are originally named
“User Test 1 - 25”. It will be possible to rename the test, select a wavelength, enter
a new calibration, select the number of decimal places used to display the results,
and select the units. A User Test may be added for a reagent system for which no
precalibrated test exists. A calibration of a LaMotte reagent system may also be
entered. The calibration of a User Test can be changed at any time.
The User Tests have the ability to handle 2 data points. The colorimeter will determine
the absorbance of the standards and calculate a response that will be stored to
determine the concentration of future samples of unknown concentration. These
standards should cover all the concentrations for the range of the test being
performed and be scanned beginning with the low concentration and fi nishing with the
high concentration (for more information about this, see CALIBRATION CURVES, page
16). Prepare these standards prior to entering a new calibration.
Editing/Set Up
NOTE: A calibration procedure must be performed before using any of the User Tests.
The User Tests can be placed in any of the alterable sequences using Edit Sequences.
1. Press and briefl y hold to
turn the meter on. The LaMotte
logo screen will appear for about
3 seconds and the Main Menu
Testing Menu
Editing Menu
Main Menu
will appear.
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 45
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 45
2. Press or to scroll to
the Editing Menu.
Main Menu
Testing Menu
Editing Menu
12:00:00 001/500
3. Press
ENTER
to select Editing
Menu. Press to scroll to
Edit User Test.
Editing/Set Up
4. Press
ENTER
to select Edit User
Test.
5. Press or to scroll to
the desired user test.
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
Edit User Test
105 USER TEST 01
106 USER TEST 02
107 USER TES
T 03
108 USER TEST 04
12:00:00 001/500
Edit User Test
108 USER TEST 04
109 USER TEST 05
110 USER TEST 06
111 USER TEST 07
12:00:00 001/500
6. Press
Test.
ENTER
to select the User
Name the Test
111 USER TEST 07
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
46 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 46
NAMING THE TEST
A User Test can be up to 16 characters long. The menu choices for each character are
26 upper case letters A to Z, 26 lower case letters a to z, ten numerals 0 to 9, a space,
a dash (-) and a decimal point (.). The existing name is displayed on the bottom line
of the display. The character which is to be edited will blink and that character is also
displayed in the center of the display. The character can be changed by using
or to scroll to other characters. Use
name is saved at any time by pressing
sixteenth character.
ENTER
EXIT
or by pressing
to select a character. The edited
ENTER
after selecting the
1. From the User Test menu, press
to scroll to Name the
Test.
2. Press
ENTER
to select Name
the Test. A blinking reverse
font (dark background with a
light character) will appear to
indicate the character that will
be adjusted. The same character
will also appear in the center of
the display.
3. Press or to scroll
to the desired character. In this
example, the test name will be
H2O.
The character for a blank space
is located after the lower case
z.
111 USER TEST 07
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
Name the Test
P Q R S T U V W X Z
111 U SER TEST 07
12:00:00 001/500
Name the Test
C D E F G H I J K L M
111 U SER TEST 07
12:00:00 001/500
Editing/Set Up
SMART3 Soil Colorimeter 9.18 47
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 47
4. Press
ENTER
to save the
character and move to the next
character.
Name the Test
N O P Q R S T U V W X
111 H S ER TEST 07
12:00:00 001/500
5. Press or to scroll to
the desired character.
Editing/Set Up
6. Press
ENTER
to save the
character and move to the next
character.
7. Press or to scroll to
the desired character.
Name the Test
X Y Z . 0 1 2 3 4 5 6 7
111 H S ER TEST 07
12:00:00 001/500
Name the Test
9 A B C D E F G H I J K
111 H2 E R TEST 07
12:00:00 001/500
Name the Test
J K L M N O P Q R S T
111 H2 O R TEST 07
12:00:00 001/500
48 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 48
8. Press
ENTER
to save the
character. Repeat the procedure
until the test name is complete.
After selecting the last
character in the name:
Hold down and press
and release
ENTER
to save all
selected characters and to
delete all characters to the right
of the cursor and exit the name
edit screen.
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
Hold down and press
and release
ENTER
to save all
remaining characters and exit
the name edit screen.
THe meter will return to the Edit
Test manu.
SELECT THE VIAL AND WAVELENGTH
The Smart3 Colorimeter accepts three diff erent vials (the 25 mm 0290 tube, UDVs and
COD tubes) at 4 diff erent wavelengths (428, 525, 560, and 635 nm). The colorimeter
uses diff erent settings for each of the twelve combinations of vial and wavelength.
1. From the User Test menu, press
or to scroll to
Select Vial/WL.
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
111 H2O
Editing/Set Up
2. Press
Vial/WL.
ENTER
to select Select
Ch1 428nm 25mm
Select Vial/WL
Ch2 525nm 25mm
Ch3 635nm 25mm
Ch4 568nm 25mm
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 49
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 49
3. Press or to
scroll to the channel with the
desired wavelingth and vial size
combination. The screen will
display storing... for about 1
second and the meter will return
to the User Test menu.
Select Vial/WL
Ch1 428nm 25mm
Ch2 525nm 25mm
Ch3 635nm 25mm
Ch4 568nm 25mm
12:00:00 001/500
4. Press
ENTER
to select the Vial/
WL option. The screen will
display Storing... for about 1
second and the meter will return
to the Edit Test menu.
Name the Test
Select Vial/WL
STD Calibration
111 H2O
Enter Constants
12:00:00 001/500
Editing/Set Up
ENTERING A TWO POINT CALIBRATION
The Smart3 Colorimeter can scan two reacted standards and create a calibration
curve. To create a calibration curve with multiple data points see Entering a Multiple
Point Calibration Curve (pg. 54).
1. From the User Test menu, press
or to scroll to STD
(Standard) Calibration.
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
111 H2O
2. Press
ENTER
to select STD
Low Standard
Calibration. The values for the
standards will be entered. The
screen will display the Low
Standard screen. A blinking
6 7 8 9 . - 0 1 2 3 4 5
0 0.000000
reverse font (dark background
with a light character) will
appear to indicate the character
12:00:00 001/500
that will be adjusted. The same
character will also appear in the
center of the display.
50 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 50
3. Press or to scroll
to the fi rst character of the low
concentration. In this example,
1.00 ppm.
Low Standard
7 8 9 . - 0 1 2 3 4 5 6
0 .000000
12:00:00 001/500
4. Press
ENTER
to save the
character and move to the next
character.
5. Press or to scroll to
the desired character.
6. Press
ENTER
to save the
character and move to the next
character.
Low Standard
5 6 7 8 9 . - 0 1 2 3 4
1 . 000000
12:00:00 001/500
Low Standard
5 6 7 8 9 . - 0 1 2 3 4
1 . 000000
12:00:00 001/500
Low Standard
6 7 8 9 . - 0 1 2 3 4 5
1. 0 00000
12:00:00 001/500
Editing/Set Up
7. Press or to scroll to
Low Standard
the desired character.
6 7 8 9 . - 0 1 2 3 4 5
1. 0 00000
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 51
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 51
8. Press
ENTER
to save the
character. Repeat the procedure
until the low concentration value
is complete. (the character for
a blank space is located after
the 9.) After the fi nal character
is complete the meter will save
the low concentration value.
The screen will display the High
Standard screen. A blinking
reverse font (dark background
with a light character) will
appear to indicate the character
that will be adjusted. The same
character will appear in the
center of the display.
High Standard
6 7 8 9 . - 0 1 2 3 4 5
0 .0000
12:00:00 001/500
9. Use or and
Editing/Set Up
to select the characters for the
high concentration value. In this
example, 7.5 ppm.
10. After the fi nal character is
entered the meter will save the
high concentration value. The
screen will display instructions
for completing the calibration
procedure.
11. The standards wil be scanned.
Insert the blank. Press
ENTER
and the Insert Low Standard
screen will appear.
ENTER
High Standard
6 7 8 9 . - 0 1 2 3 4 5
7.50000 0
12:00:00 001/500
STD Calibration
Insert Blank
nter> continue
<E
12:00:00 001/500
STD Calibration
Insert Low Standard
nter> continue
<E
12:00:00 001/500
52 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 52
12. Insert the low standard.
ENTER
Press
. The Insert
High Standard screen will be
displayed.
STD Calibration
Insert High Standard
nter> continue
<E
12:00:00 001/500
13. Insert the high standard. Press
ENTER
. The meter will display
the constants for the calibration
curve.
14. Press
ENTER
to save the
calibration curve and return to
the Edit User Test Menu.
K0 = 0.57246
K1 = 7.14547
K2 = 0.00000
K3 = 0.00000
<Enter> Save
12:00:00 001/500
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
Editing/Set Up
SMART3 Soil Colorimeter 9.18 53
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 53
ENTERING A MULTIPLE POINT CALIBRATION
The Smart3 can directly create a 2 point calibration curve. (See Entering a Two Point
Calibration on page 50.) To create a multiple point calibration curve, constants
obtained from a linear regression of multiple data points can be entered into the
Smart3.
1. Scan reactions of multiple concentrations at the appropriate wavelength in the
absorbance mode on the Smart3.
2. Plot the concentration (y axis) versus absorbance (x axis) in a program
capable of linear regression such as Excel.
3. Enter the constants obtained from the linear regression equation into the
Smart3.
For Example:
y = 0.001x3 – 0.017x2 + 0.181x – 0.049
K0 = – 0.049
Editing/Set Up
K1 = 0.181
K2 = – 0.017
K3 = 0.001
OR (Over Range) = 10
1. From the User Test menu, press
or to scroll to Enter
Constants.
Name the Test
111 H2O
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
2. Press
Constants.
ENTER
to select Enter
K0=0.00000
K1=1.00000
K2=0.00000
K3=0.00000
OR=100.00000
12:00:00 001/500
54 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 54
3. Press
ENTER
to begin entering
the values for the constants. A
reverse font (dark background
with a light character) will
appear to indicate the character
that will be adjusted. The same
character will also appear in the
center of the display.
Enter Constants K0
6 7 8 9 . - 0 1 2 3 4 5
0 .00000
12:00:00 001/500
4. Press or to scroll to
the fi rst character of K0. In this
example, – 0.049.
5. Press
ENTER
to save the
character and move to the next
character.
6. Press or to scroll to
the next character.
Enter Constants K0
6 7 8 9 . - 0 1 2 3 4 5
0 .00000
12:00:00 001/500
Enter Constants K0
5 6 7 8 9 . - 0 1 2 3 4
– . 00000
12:00:00 001/500
Enter Constants K0
6 7 8 9 . - 0 1 2 3 4 5
– . 00000
12:00:00 001/500
Editing/Set Up
7. Press
ENTER
to save the
Enter Constants K0
character and move to the next
character. Press or
to scroll to the next character.
5 6 7 8 9 . - 0 1 2 3 4
– 0 0 0000
Note: The character for a blank
space is located after the 9.
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 55
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 55
8. Press
ENTER
to save the
character. Repeat the procedure
until the K0 value is complete.
After the fi nal character is
complete the meter will save
the K0 value and the meter will
display K1 screen.
Enter Constants K1
6 7 8 9 . - 0 1 2 3 4 5
0 .00000
12:00:00 001/500
9. Use , and
to select the characters for the
remaining values: K1, K2, K3,
and over range. In this example,
10 ppm.
Editing/Set Up
10. After the fi nal character is
entered the meter will save
the constants. The screen will
display Storing... and return to
the Edit Test menu.
ENTER
Constants OR
6 7 8 9 . - 0 1 2 3 4 5
10.00000 0
12:00:00 001/500
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
56 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 56
SELECTING THE NUMERICAL FORMAT OF THE RESULT
To input tests with very diff erent ranges, the number of decimal places displayed for
a result can be selected. A test which ranges from 20 to 1000 ppm should not be
displayed with three decimal places. A test with a range from 0.010 to 0.500 needs
three decimal places (the microprocessor will always calculate the concentration
to many more signifi cant fi gures than will be displayed). The choice of 0, 1, 2, or 3
decimal places are available.
1. From the User Test menu, press
or to scroll to
Decimal Places.
2. Press
ENTER
to select Decimal
Places.
3. Press or to scroll to
the desired number of decimal
places.
111 H2O
Select Vial/WL
STD Calibration
Enter Constants
Decimal Places
12:00:00 001/500
Decimal Places
None 0
One 0.0
Two 0.00
Three 0.000
12:00:00 001/500
Decimal Places
None 0
One 0.0
Two 0.00
Three 0.000
12:00:00 001/500
Editing/Set Up
4. Press
ENTER
to select the
decimal places. The screen wil
display Storing... for about 1
second and the meter will return
to the Edit Test menu.
Select Vial/WL
STD Calibration
Enter Constants
111 H2O
Decimal Places
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 57
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 57
SELECTING THE UNITS OF CONCENTRATION
The Smart3 Colorimeter has seven options for units of concentration. They are No
Units, ppm, pH, FAU, ppb, ppt and mgL.
1. From the User Menu, press
to scroll to Select
Units.
2. Press
ENTER
to select Select
Units.
Editing/Set Up
3. Press or to scroll to
the desired units.
111 H2O
STD Calibration
Enter Constants
Decimal Places
Select Units
12:00:00 001/500
Select Units
No Units
ppm
pH
FAU
12:00:00 001/500
Select Units
No Units
ppm
pH
FAU
12:00:00 001/500
4. Press
ENTER
to select the units.
The screen will display Storing...
for about 1 second and the
meter will return to the Edit Test
menu.
STD Calibration
Enter Constants
Decimal Places
111 H2O
Select Units
12:00:00 001/500
58 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 58
SETTING THE CLOCK
Setting the clock allows the correct time and date stamp to be stored with each
reading in the data logger.
1. From the Editing Menu, press
or to scroll to Set
Clock.
2. Press
ENTER
to select Set Clock.
The year is displayed. Press
or to scroll to the
appropriate character. Press
ENTER
to select the character.
The month, day, hour, format
hour, minute, second, AM/PM
will be dislpayed. Repeat for
each.
3. Press
ENTER
to select the fi nal
character. The time and date
will be saved and the meter will
return to the Editing Menu.
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
Set Time
Year: 2000
12:00:00 001/500
Editing Menu
Edit Sequences
dit User Test
E
Set Clock
Logging
12:00:00 001/500
Editing/Set Up
SMART3 Soil Colorimeter 9.18 59
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 59
LOGGING DATA
The default setting for the data logger is enabled. The meter will log the last 500 data
points. The counter in the center bottom of the display will show how many data points
have been logged. The display will show 500+ when the data logger has exceeded 500
points and the data points are being overwritten.
1. From the Editing Menu, press
or to scroll to
Logging.
2. Press
ENTER
to select
Logging.
Editing/Set Up
3. Press or to scroll to
desired function.
Editing Menu
Edit Sequences
Edit User Test
Set Clock
Logging
12:00:00 001/500
Logging
Display Test Log
Logging Enabled
ogging Disabled
L
Erase Log
12:00:00 001/500
Logging
Display Test Log
Logging Enabled
Logging Disabled
rase Log
E
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and return to the Editing
Menu.
Edit Sequences
Edit User Test
Editing Menu
Set Clock
Logging
12:00:00 001/500
60 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 60
FACTORY SETUP
The Factory Setup menu is used in manufacturing of the Smart3 Colorimeter. This
menu is not for use by the operator in the fi eld.
SETTING POWER SAVE
The power saving Auto Shutoff feature will turn the meter off when a button has not
been pushed for a set amount of time. The default setting is disabled. To change the
setting:
1. From the Editing Menu, press
or to scroll to Set
Power Save.
2. Press
ENTER
to select Set PWR
Save.
3. Press or to scroll to
desired function.
Editing Menu
Set Clock
Logging
Factory Setup
Set Power Save
12:00:00 001/500
Set PWR Save
Disable
5 Minutes
15 Minutes
30 Minut
12:00:00 001/500
es
Set PWR Save
Disable
5 Minut
es
15 Minutes
30 Minutes
12:00:00 001/500
Editing/Set Up
4. Press
ENTER
. The screen will
display Storing.... for about 1
second and the meter will retun
to the Editing Menu.
Set Clock
Logging
Editing Menu
Factory Setup
Set PWR Save
12:00:00 001/500
SMART3 Soil Colorimeter 9.18 61
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 61
SETTING THE BACKLIGHT TIME
The backlight illuminates the display for enhanced viewing. The default setting is 10
seconds. If Button Control is chosen the backlight button on the key pad will act as
an on/off switch and the backlight will remain on or off when the meter is being used.
When one of the other settings – 10, 20 or 30 seconds – is chosen, the display will be
illuminated for the specifi ed amount of time after any button is pressed.
NOTE: The backlight feature uses a signifi cant amount of power. The longer the
backlight is on, the more frequently the battery will have to be charged if the
USB/Wall Adapter is not being used.
1. From the Editing Menu, press
or to scroll to
Backlight Time.
Editing/Set Up
2. Press
ENTER
to select Set
Backlight Time.
3. Press or to scroll to
desired option.
Editing Menu
Logging
Factory Setup
Set Power Save
Set Backlight Time
12:00:00 001/500
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
Set Backlight Time
Button Control
10 seconds
20 seconds
30 seconds
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
Logging
Factory Setup
Editing Menu
Set Power Save
Set Backlight Time
12:00:00 001/500
62 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 62
BLUETOOTH MENU
Enabling Bluetooth Power
Bluetooth wireless technology allows communication between the colorimeter and a
Bluetooth Mobile Printer (Code 5-0066 only). Bluetooth Power must be enabled to use
the Bluetooth Mobile Printer (Code 5-0066). When Bluetooth Power is enabled, the
Bluetooth icon will fl ash next to the battery icon on the bottom line of the display.
If the Bluetooth feature is not being used to connect to the printer, Bluetooth Power
should be disabled to conserve the battery life. The default Bluetooth Power setting is
disabled.
1. From the Editing Menu, press
or to scroll to
Bluetooth Menu.
2. Press
ENTER
to select Bluetooth
Menu.
3. Press or to scroll to
Bluetooth Power.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Bluetooth Menu
12:00:00 001/500
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500
Editing/Set Up
SMART3 Soil Colorimeter 9.18 63
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 63
4. Press
ENTER
Bluetooth Power.
to select
Bluetooth Power
Enable Bluetooth
Disable Bluetooth
12:00:00 001/500
5. Press or to scroll to
desired option.
Editing/Set Up
6. Press
ENTER
to select the option.
Bluetooth Power must be
enabled to use the Bluetooth
Mobile Printer (Code 5-0066).
The screen will display
Storing… for about 1 second
and the meter will return to the
Bluetooth Menu.
A fl ashing P symbol will
indicate that Bluetooth is
enabled.
Bluetooth Power
Enable Bluetooth
Disable Bluetooth
12:00:00 001/500
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
64 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 64
Setting The Bluetooth Mode
A Bluetooth connection is available only for the Bluetooth Mobile Printer (Code
5-0066).
1. From the Editing Menu, press
or to scroll to
Bluetooth Menu.
2. Press
ENTER
to select Bluetooth
Menu.
3. Press or to scroll to
Bluetooth Mode.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Bluetooth Menu
12:00:00 001/500 P
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
Editing/Set Up
12:00:00 001/500 P
4. Press
Bluetooth Mode.
ENTER
to select
Mobile Device
Bluetooth Mode
BT Printer
12:00:00 001/500 P
SMART3 Soil Colorimeter 9.18 65
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 65
5. Press or to scroll
to BT Printer.
Bluetooth Mode
Mobile Device
BT Printer
12:00:00 001/500 P
6. Press
ENTER
. The screen will
display Storing… for about 1
second and the meter will return
to the Bluetooth Menu.
Bluetooth Power
Bluetooth Mode
Bluetooth Menu
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
Scan For Printers
The Smart3 can only be used with Bluetooth Mobile Printer (Code 5-0066).
1. Turn on the power to the
Bluetooth Mobile Printer (Code
5-0066).
2. From the Editing Menu, press
or to scroll to
Bluetooth Menu.
Factory Setup
Set Power Save
Set Backlight Time
Bluetooth Menu
Editing Menu
12:00:00 001/500 P
66 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 66
3. Press
Bluetooth Menu.
ENTER
to select
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
4. Press or to scroll
to Bluetooth Scan.
5. Press
ENTER
to select
Bluetooth Scan. The meter will
begin scanning. A count down
timer will begin counting down
from 11 seconds.
If “No BT Available…” is
displayed, Bluetooth is not
enabled on the meter. Go to
Bluetooth Power to enable it.
Bluetooth Menu
Bluetooth Power
Bluetooth Mode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
Bluetooth Scan
Scanning (11)...
12:00:00 001/500 P
SMART3 Soil Colorimeter 9.18 67
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 67
6. The addresses of the available
printers will be displayed.
NOTE: The printer address
for the Bluetooth Mobile
Printer (Code 5-0066) can be
determined by turning the power
off and then pressing the Power
button and the Paper Feed
button simultaneously to print
the printer specifi cations. The
printer address is designated as
Bluetooth: BT Address.
Select Printer
000D163140A0
Set Backlight Time
12:00:00 001/500 P
7. Scroll to the desired printer and
press ENTER to pair the printer.
The screen will display the BT
Address and Working… for
000D163140A0
Connecting
Connect to Printer
about 1 second. The meter will
display a status message when
the printer is connected.
12:00:00 001/500 P
68 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 68
Set BT Timeout
The Bluetooth Timeout can be set to allow the Bluetooth feature to be on to allow data
to be sent to the Bluetooth printer and then turn off after a specifi ed amount of time.
1. From the Editing Menu, press
or to scroll to
Bluetooth Menu.
2. Press
ENTER
to select Bluetooth
Menu.
3. Press or to scroll to
Set BT Timeout.
Editing Menu
Factory Setup
Set Power Save
Set Backlight Time
Bluetooth Menu
12:00:00 001/500 P
Bluetooth Menu
Bluetooth Power
BluetoothMode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
Bluetooth Menu
Bluetooth Power
BluetoothMode
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
4. Press
Timeout.
ENTER
to select Set BT
15 minutes
Set BT Timeout
5 minutes
30 Seconds
Disable
12:00:00 001/500 P
SMART3 Soil Colorimeter 9.18 69
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 69
5. Press or to scroll
to desired option.
Set BT Timeout
15 minutes
5 minutes
30 Seconds
Disable
12:00:00 001/500 P
6. Press
ENTER
. The screen will
display Storing… for about 1
second and the meter will return
to the Bluetooth Menu.
Bluetooth Power
Bluetooth Mode
Bluetooth Menu
Bluetooth Scan
Set BT Timeout
12:00:00 001/500 P
SELECTING A LANGUAGE
There are seven languages available in the Smart3: English, Spanish, French,
Portuguese, Italian, Chinese, and Japanese.
1. From the Editing Menu, press
or to scroll to
Select Language.
Set Power Save
Set Backlight Time
Bluetooth Menu
Select Language
12:00:00 001/500
Editing Menu
2. Press
Language.
ENTER
to select Select
English
Select Language
Spanish
rench
F
Portugese
12:00:00 001/500
70 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 70
3. Press or to scroll to
desired language.
Select Language
English
Spanish
rench
F
Portugese
12:00:00 001/500
4. Press
ENTER
. The screen will
display Storing... for about 1
second and the meter will return
to the Editing Menu.
Set Power Save
Set Backlight Time
Editing Menu
Bluetooth Menu
Select Language
12:00:00 001/500
NOTE: If meter unintentionally switches to another language, use the procedure above
to reset the meter to the desired language. For example, to reset the meter to English:
Turn meter on.
1. Press one time. Press
2. Press eight times. Press
3. Press
ENTER
.
ENTER
ENTER
.
.
SMART3 Soil Colorimeter 9.18 71
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 71
LOOPING MENUS
Long menus, such as All Tests, incorporate a looping feature which allows the user to
quickly reach the last choice in the menu from the fi rst choice. In a looping menu the
last choices in the menu are above the fi rst choice and scrolling upward moves through
the menu in reverse order. Scrolling downward moves through the menu from fi rst
choice to last but the menu starts over following the last choice. So all menu choices
can be reached by scrolling in either direction. The diagrams below demonstrate a
looping menu.
AND SO ON AND SO ON AND SO ON
: : : : : : : : :
: : : : : : : : :
THIRD TO LAST LAST CHOICE LAST CHOICE
SECOND TO LAST AND SO ON AND SO ON
LAST CHOICE : : : : : :
TESTING MENU TESTING MENU TESTING MENU
FIRST CHOICE
SECOND CHOICE THIRD TO LAST THIRD TO LAST
THIRD CHOICE
ANOTHER LAST CHOICE LAST CHOICE
12:00:00 001/500 12:00:00 001/500 12:00:00 001/500
AND ANOTHER FIRST CHOICE FIRST CHOICE
AND SO ON SECOND CHOICE SECOND CHOICE
: : : THIRD CHOICE THIRD CHOICE
: : : ANOTHER ANOTHER
LAST CHOICE AND ANOTHER AND ANOTHER
: : : : : :
SECOND TO LAST SECOND TO LAST
The feature called Looping Menu can be turned on and off in the Editing Menu. The
default setting is ON.
72 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 72
COMPUTER CONNECTION
OUTPUT
USB
COMPUTER CONNECTION
USB Type A, USB mini B, Order Cable Code 1720.
BLUETOOTH CONNECTION
Barriers to wireless signals can reduce the range of wireless devices. The Smart3 will
work best if there are no walls between it and the receiving device.
The Smart3 colorimeter uses Bluetooth wireless technology to allow communication
between the colorimeter and a bluetooth printer (Code 3-0066 only).
WATERLINK CONNECT
The meter may be interfaced with any Windows-based 64 bit computer by using
the LaMotte WaterLink Connect program and a USB cable. The program will store
test information and results in a local database, and allow for exporting this data to
a comma separated value (CSV) fi le. The meter will send the following data: time/
date stamp, name of test, sample value, sample units, meter name, and location. To
download WaterLink go to http://softwarecenter.lamotte.com/. Select “WaterLink
Connect Software for Windows FREE Download”.
SMART3 Soil Colorimeter 9.18 73
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 73
BATTERY
BATTERY/AC OPERATION
The SMART3 may be operated on battery power using a USB wall adapter or USB
computer connection. If using the meter as a bench top unit, use the wall adapter if
possible to extend the battery life. The meter will remain on when the USB adapter is
used.
To charge the battery with the wall adapter, plug the smaller end of the USB cable
(USB mini B connector) into the meter and the larger end of the USB cable (USB type
A connector) into the wall adapter. Plug the wall adapter into an AC outlet. Reinsert the
rubber USB port plug after charging. To charge the battery from a computer, plug the
smaller end of the USB cable (USB mini B connector) into the meter and the larger end
of the USB cable (USB Type A connector) into a USB port on a computer. The battery
icon will show no bars and fl ash when the unit fi rst turns on. Then the indicator will
indicate the battery status by showing 0, 1, 2, 3, or 4 bars.
It will take 6 hours to fully charge a low battery. The battery icon will fl ash when the
battery is charging. The battery icon will show four bars and stop fl ashing when it is
fully charged. The charging circuit will automatically switch to a fl oat charge when the
battery is fully charged. The charger may remain connected. Some computers will NOT
supply power to their USB ports during standby operation. The wall adapter will charge
the unit continuously.
Storing the meter above ambient room temperature will decrease the battery charge
more quickly than storage at room temperature. If the meter does not turn on, it means
that the battery is at a very low charge. Charging the battery with the wall adapter in
this state may take up to 10 hours. At low temperatures, approaching 0 °C, the battery
will charge more slowly. It will not charge at all below 0 °C. The battery icon will show
no bars and continuously fl ash if the battery is getting low but the unit will still operate
normally. A “Low Battery” message on the status bar of the display will replace the time
when the battery voltage is too low for proper operation and accuracy may be degraded.
A “Shutdown Low Batt” message on the display will appear for a few seconds before the
power is switched off when the battery is too low to operate the unit. When the battery
icon simultaneously fl ashes bars 1 and 2 followed by bars 3 and 4, it is an indication
that the battery is damaged and technical support should be contacted.
To extend the battery life:
◊ Shut down the unit with the power switch when not taking measurements or use
the power save option to have the unit automatically turn off after 5 minutes.
◊ Store the unit at a moderate temperature.
◊ Fully charge the battery before storing the unit for extended periods of time.
◊ Fully charge the battery at least once per year. Failure to do so may result in a
permanently drained battery.
◊ Limit backlight use. The unit consumes 3X normal power with the backlight on. Set
the backlight time option to 10 seconds, or select “Button Control” and keep the
backlight off .
74 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 74
BATTERY REPLACEMENT
The lithium ion battery used in this unit should last for many years with normal use.
When it no longer powers the unit long enough to meet testing requirements it will need
to be replaced. Lithium ion batteries that are properly charged and stored do not usually
lose all capacity; they just have less capacity after hundreds of charge cycles. This unit
uses a custom battery assembly that is only available from LaMotte Company. Battery
replacement must be performed at a LaMotte authorized repair facility. The water
resistant housing of this meter should not be opened by the user. Contact LaMotte
Company by phone (1-800-344-3100) or email (tech@lamotte.com) for a return
authorization number.
MAINTENANCE
CLEANING
Clean the exterior housing with a damp, lint-free cloth. Do not allow water to enter the
light chamber or any other parts of the meter. To clean the light chamber and optics
area, point a can of compressed air into the light chamber and blow the pressurized air
into the light chamber. Use a cotton swab dampened with Windex® window cleaner to
gently swab the interior of the chamber. Do not use alcohol; it will leave a thin residue
over the optics when dry.
REPAIRS
Should it be necessary to return the meter for repair or servicing, pack the meter
carefully in a suitable container with adequate packing material. A return authorization
number must be obtained from LaMotte Company by calling 800-344-3100 (US only) or
410-778-3100, faxing 410-778-6394, or emailing tech@lamotte.com. Often a problem
can be resolved over the phone or by email. If a return of the meter is necessary, attach
a letter with the return authorization number, meter serial number, a brief description
of problem and contact information including phone and FAX numbers to the shipping
carton. This information will enable the service department to make the required repairs
more effi ciently.
METER DISPOSAL
Waste Electrical and Electronic Equipment (WEEE)
Natural resources were used in the production of this equipment. This equipment may
contain materials that are hazardous to health and the environment. To avoid harm to
the environment and natural resources, the use of appropriate take-back systems is
recommended. The crossed out wheeled bin symbol on the meter encourages the use of
these systems when disposing of this equipment.
SMART3 Soil Colorimeter 9.18 75
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 75
Take-back systems will allow the materials to be reused or recycled in a way that will
not harm the environment. For more information on approved collection, reuse, and
recycling systems contact local or regional waste administration or recycling services.
TROUBLESHOOTING
ERROR MESSAGES
OVER RANGE
If the message OVERRANGE is displayed when scanning a sample, the sample may be
over range or under range. If the sample is over range the sample should be diluted and
tested again (see Sample Dilution Techniques and Volumetric Measurements, page 14).
If overrange is displayed, press
ENTER
to continue testing on diluted
samples.
Note: After pressing
overrange cncentration will be
displayed. This concentration is an
approximation only.
ENTER
, the
Overrange
<Enter> continue
Scan Blank
Scan Sample
12:00:00 001/500
005 Aluminum
CALIBRATION
As with all pre-calibrated meters, it is highly recommended, even if not required by
regulations, that the user periodically verify the performance of the meter by running
standards with a predetermined concentration. Results outside of specifi cation are
an indication that the meter needs to be adjusted. This can be done following the user
calibration described on page 24. If the user calibration fails to properly adjust the
meter then the meter should be returned to LaMotte Company for recalibration. (See
page 75).
STRAY LIGHT
The Smart3 Colorimeter should have no problems with stray light. Make sure that
the sample compartment lid is always fully closed, except when testing COD with the
adapter.
76 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 76
TROUBLESHOOTING GUIDE
PROBLEM REASON SOLUTION
Flashing
“Low Battery” Battery voltage is very
“Shut Down Low Batt”
Shut Down
“Overrange” Sample is outside of
Low battery. Readings
are reliable.
low. Readings are not
reliable.
Battery is too low to
operate the unit.
acceptable range.
Charge battery or use
USB wall/computer
adapter.
Charge battery or use
USB wall/computer
adapter.
Charge battery or use
USB wall/computer
adapter.
Dilute sample and test
again.
Unusually large
negative or positive
readings when
performing calibration
Incorrect standards
used to calibrate meter.
Use fresh 0.0 standard
in clean tube. Reset
meter to factory default
settings. Recalibrate
meter.
SOFTWARE TROUBLESHOOTING
PROBLEM REASON SOLUTION
No printer connected Colorimeter has
not been paired via
Bluetooth, or pairing
was lost by leaving
Bluetooth range.
Printer does not
appear in the list of
Bluetooth devices
Connection lost Bluetooth pairing with
Printer in not powered
on.
the printer was lost.
Re-pair the colorimeter
to the printer.
Power on the printer
Re-pair the Printer to the
colorimeter
SMART3 Soil Colorimeter 9.18 77
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 77
SMART3 Soil
Colorimeter
Test
Procedures
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 78
SMART3 SOIL COLORIMETER REAGENT SYSTEMS
SMART3 REAGENT SYSTEMS LIST
LaMotte Company continuously updates the list of pre-programmed tests as the
calibrations become available.
Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA)
or email at tech@lamotte.com for a current list of available calibrations and more
information.
# of
Test Factor (Test #) Range (ppm) Test Method (# of Reagents)
Ammonia NitrogenHigh Range (008)
Calcium & Magnesium 0-4000
Chloride 0-1000 Direct Reading Titrator (2) 50
Copper-Thiocarbamate (036) 0.00–6.00 Diethyldithiocarbamate (1) 50
Copper-UDV (037) 0.0–4.0 Bicinchoninic Acid (1) 100
Iron-Bipyridyl (055) 0.00–6.00 Bipyridyl (2) 50
Manganese-High Range (059) 0.0–15.0 Periodate (2) 50
Nitrate Nitrogen-Low Range (065) 0.00–3.00 Cadmium Reduction (2) 20
Nitrite Nitrogen-Low Range (068) 0.00–0.80 Diazotization (2) 20
Phosphate-Low Range (081) 0.00–3.00 Ascorbic Acid Reduction (2) 50
Potassium (085) 0.0-10.0 Tetraphenylboron (2) 100
Sulfur (089) 0–100 Barium Chloride (1) 50
Zinc-Low Range (097) 0.00–3.00 Zincon (6) 50
0.00–4.00 Nesslerization (2) 50
Schwarzenbach EDTA (8) 50
Calcium
0-2400
Magnesium
Tests
80 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 79
ELECTRONIC TEST METHODS
pH
PROCEDURE
1. Use the 10 g Soil Measure (1164) to add one level measure of the soil sample to a
50 mL beaker (0944). Use the graduated cylinder (0416) to add 10 mL of deionized
water. Stir thoroughly.
2. Let stand for at least 30 minutes, stirring two or three times.
3. Stir mixture just prior to making the pH reading. Determine the pH reading of the
sample by following the instructions for the pH Meter.
LIME REQUIREMENT - WOODRUFF METHOD
PROCEDURE
1. Use the 10 g Soil Measure (1164) to add one level measure of the soil sample to a
50 mL beaker (0944). Use the graduated cylinder (0416) to add 10 mL of deionized
water. Stir thoroughly.
2. Let stand for at least 15 minutes.
3. Add 20 mL of Woodruff Buff er Solution (5272). Mix well, and let stand for at least 20
minutes, stirring two or three times.
Testing
4. Take reading using the pH meter. Stir mixture just prior to making reading.
5. Each 0.1 pH unit drop from pH 7.0 indicates a lime requirement equivalent to 1000
lbs calcium carbonate (CaCO3)/acre.
SMART3 Soil Colorimeter 9.18 81
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 80
SOLUBLE SALTS (TOTAL DISSOLVED SALTS)
Most plants will get along well at soluble salts concentrations of below 1000 parts per
million. However, greenhouse and many sensitive garden plants may be damaged if
the soluble salts are over 500 parts per million of chlorides, particularly some of the
most sensitive legumes. If the soluble salts are greater that 1000 parts per million,
the chlorides and sulfates should be determined to learn whether the soluble salts are
Testing
chlorides or sulfates. In calcareous soils, the sulfates represent gypsum and have little
eff ect on the production of plants.
PROCEDURE
1. Fill a 50 mL beaker (0944) with the soil to be tested, tapping it lightly to eliminate
any trapped air and then strike off the surface.
2. Empty the contents of the beaker into the 300 mL bottle (0991). Add 100 mL of
deionized water.
3. Cap the bottle and shake vigorously. Allow to stand for thirty minutes. During the
thirty minute waiting period the bottle should be shaken vigorously three or four
times.
4. Filter the contents of the bottle using funnel (0459) and fi lter paper (0463) and
collect the fi ltrate in a 100 mL bottle (0990) which is then used as a conductivity
chamber.
5. Determine the TDS reading of the sample by following the instructions for the TDS
Meter.
6. To convert conductivity to Soluble Salts (Total Dissolved Solids), use the following
formula.
ppm Soluble Solids (Total Dissolved Solids) = Micromhos/cm @ 25°C x 0.7
82 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 81
EXTRACTION PROCEDURE
The following method of extraction is employed for obtaining the soil fi ltrate for the
tests for Nitrate Nitrogen, Phosphorous, Potassium, Calcium, Magnesium, Ammonia
Nitrogen, Nitrite Nitrogen, Manganese, Copper, Zinc, and Iron. Separate extractions are
required for the Chloride and Sulfate tests. Consult the LaMotte Soil Handbook (1504)
for information on sampling and preparation of sample for testing.
MULTIPLE TEST PROCEDURE
1. Use the 1 mL pipet (0354) to add 5 mL of *Acid Extracting Solution (6361) to the
100 mL graduated cylinder (0419). Add deionized water to 75 mL graduation.
2. Pour this solution into the 100 mL bottle (0990).
3. Use the Soil Measure (1165) to add 15 g (one level measure) of the soil sample to
the bottle.
4. Cap the bottle and shake for 5 minutes.
5. Use the funnel (0459) and fi lter paper (0463) to fi lter and collect all of the soil
extract in a 100 mL bottle (0990).
6. The soil extract is used for all of the tests listed above, except Chloride and
Sulfate.
SINGLE TEST PROCEDURE
1. Use the 1 mL pipet (0354) to add 1 mL of *Acid Extracting Solution (6361) to the
test tube (0701), then add deionized water to fi ll to the 15 mL line.
Testing
2. Use the 1.0 g spoon (0697) to add 3 measures of soil to the extracting solution in
the test tube.
3. Cap the tube and shake for 5 minutes.
4. Filter, using the funnel (0459) and fi lter paper (0463) and collect all of the soil
extract.
5. The soil extract is used for all of the tests listed above except Chloride and
Sulfate.
SMART3 Soil Colorimeter 9.18 83
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 82
NEUTRALIZATION OF SOIL FILTRATE
In the test procedures for Ammonia Nitrogen, Calcium & Magnesium, Copper, Iron,
Manganese and Zinc require that the acidity of the soil extract be neutralized before the
test procedure is performed. This is done by adding *Sodium Hydroxide, 15% (7886) to
the soil extract until Bromthymol Blue Test Paper (2931) indicates that the pH is in the
proper range.
Testing
1. Add one drop of *Sodium Hydroxide, 15% (7886) to the soil extract. Stir with the
stirring rod.
2. Touch the stirring rod to the Bromthymol Blue Test Paper (2931).
3. If the test paper does not change from yellow to blue or green, continue adding
*Sodium Hydroxide, 15% to the soil extract, one drop at a time. Stir and test the pH
after the addition of each drop until the test paper changes from yellow to green or
blue.
84 SMART3 Soil Colorimeter 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 83
AMMONIA NITROGEN
NESSLERIZATION METHOD • CODE 3642-SC
QUANTITY CONTENTS CODE
30 mL Ammonia Nitrogen Reagent #1 V-4797-G
2 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G
1 Pipet, 1 mL, plastic 0354
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
A fertile soil may be expected to give a low ammonia nitrogen test reading, unless there
has been a recent application of nitrogenous fertilizer in forms other than the nitrate.
The rapid disappearance of ammonia after fertilizer application indicates the desired
transformation of the ammonia to the more available nitrate compounds. In forest soils,
ammonia is the most abundant available form of nitrogen. If there is a satisfactory
rate of nitrogen transformation, the humus layers of a forest soil will produce very high
concentrations of ammonia nitrogen.
Testing
RANGE: 0.00-200.00 lb/acre Ammonia Nitrogen
METHOD: Ammonia forms a colored complex with Nessler’s
Reagent in proportion to the amount of ammonia
present in the sample. Rochelle salt is added to prevent
precipitation of calcium or magnesium in undistilled
samples.
INTERFERENCES: Sample turbidity and color may interfere. Turbidity may
be removed by a fi ltration procedure. Color interference
may be eliminated by blanking the instrument with a
sample blank.
Smart3 Soil Test Procedures 9.18 AMMONIA NITROGEN
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 84
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Scroll to and select All Tests (or another sequence containing 008 Ammonia-N HR)
Testing
4. Scroll to and select 008 Ammonia-N HR from menu.
5. Use the 1 mL pipet (0354) to transfer 2 mL of soil extract into a clean tube (0290).
6. Insert tube into chamber, close lid and select Scan Blank. (See Note)
7. Remove tube from colorimeter. Add 12 drops of Ammonia Nitrogen Reagent #1 (V-
8. Use the 1.0 mL pipet (0354) to add 1.0 mL of *Ammonia Nitrogen Reagent #2 (V-
9. At end of the 5 minute waiting period, immediately mix, insert tube into chamber,
ENTER
to select Testing Menu.
from Testing Menu.
Dilute to the 10 mL line with deionized waer. Mix and neutralize according to the
procedure on page 84.
4797). Cap and mix. Wait 1 minute.
4798). Cap and mix. Allow 5 minutes for maximum color development.
close lid and select Scan Sample. Multiply the result by 50 to determine the
ammonia-nitrogen concentration in lb/acre.
10. Press to turn the colorimeter off or press the
EXIT
exit to a previous menu
or make another menu selection.
NOTE: It is strongly suggested that a reagent blank be determined to account for any
contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This
test result is the reagent blank. Subtract the reagent blank from all subsequent test
results of unknown samples. It is necessary to determine the reagent blank only when a
new lot number of reagents is obtained.
Ammonia Nitrogen Concentration Chart
Pounds per Acre Range
0-24 lb/acre Low
25-68 lb/acre Medium
Over 71 lb/acre High
AMMONIA NITROGEN Smart3 Soil Test Procedures 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 85
CALCIUM & MAGNESIUM
SCHWARZENBACH EDTA METHOD • CODE M-CAL-MAG
QUANTITY CONTENTS CODE
30 mL *Calcium & Magnesium Buff er *5126-G
60 mL Standard EDTA Reagent 5254-H
50 Calcium Hardness Indicator Tablets 5250A-H
30 mL Calcium Magnesium Inhibitor Reagent 3922-G
30 mL *CM Indicator Reagent *6522WT-G
30 mL *Sodium Hydroxide w/Metal Inhibitors *4259-G
15 mL *Inhibitor Solution *9258-E
15 mL *Triethanolamine Reagent *3921-E
2 Direct Reading Titrators, 0-1000 Range 0384
1 Pipet, transfer, plastic 0364
1 Test tube, 5-10-15 mL, glass, w/cap 0778
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Testing
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
The amount of total calcium in soils may range from as little as 0.1% to as much 25%.
A calcium defi ciency is rarely a problem due to widely accepted practice of applying
lime to soil to raise the pH to the proper range for optimum plant growth. As an
important mineral nutrient, calcium is a component of cell walls in plants and is known
to stimulate root and leaf development as well as activate several enzyme reactions
involved in plant metabolism. Indirectly, calcium infl uences crop yields by reducing soils
acidity and by reducing the toxicity of several other soil minerals such as manganese,
zinc, and aluminum.
The Schwarzenbach EDTA titration method, used to determine calcium and magnesium,
involves two titrations. The fi rst titration gives the calcium and magnesium content, the
second only calcium. Magnesium is calculated from the diff erence between the titration
values.
Smart3 Soil Test Procedures 09.18 CALCIUM & MAGNESIUM
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 86
RANGE: 0-4000 lb/acre Calcium
0-2400 lb/acre Magnesium
METHOD: Titration with Schwarzenbach EDTA
INTERFERENCE: Sample color and turbidity may interfere with endpoint.
Testing
PROCEDURE
I. DILUTION OF SOIL EXTRACT
Use the 30 mL graduated cylinder (0418) to measure 10 mL of the soil extract and
transfer it to a 50 mL beaker (0944). Add 10 mL of deionized water, mix and neutralize
according to the procedure on page 84.
II. TITRATION A, CALCIUM & MAGNESIUM
Carefully read the LaMotte Direct Reading Titrator Manual (1649) before performing the
titrations described below.
1. Fill the test tube (0778) to the 5 mL line with the soil extract from above. Dilute to
the 10 mL line with deionized water.
2. Add 5 drops of Calcium Magnesium Inhibitor Reagent (3922).
3. Wait 5 minutes.
4. Use a pipet (0364) to add 5 drops of *Calcium & Magnesium Buff er (5126).
5. Add 10 drops of *CM Indicator (6522WT).
6. Fill the Direct Reading Titrator (0384) with the Standard EDTA Reagent (5254).
Insert the tip of the Titrator into the center hole of the test tube cap.
7. While gently swirling the tube, slowly press the plunger to titrate until the color
changes from red to blue and does not revert to red upon standing for 1 minute.
8. Read the test result from the scale where the large ring on the Titrator meets the
Titrator barrel. Multiply by 5.16. This is Titration Value A.
III. TITRATION B, CALCIUM
1. Fill the test tube (0778) to the 5 mL line with the diluted soil extract. Dilute to 10
mL with deionized water.
2. Add 2 drops of *Inhibitor Solution (9258).
3. Add 2 drops of *Triethanolamine Reagent (3921).
4. Add 8 drops of *Sodium Hydroxide w/Metal Inhibitors (4259).
CALCIUM & MAGNESIUM Smart3 Soil Test Procedures 09.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 87
5. Add one Calcium Hardness Indicator Tablet (5250A) to the test sample. Cap and
shake to dissolve the tablet. A red color will develop.
6. Immediately titrate the sample. Fill the Direct Reading Titrator with Standard EDTA
Reagent (5254). Insert the tip of the Titrator into the hole in the cap of the test
tube.
7. While gently shaking the tube, slowly press the plunger to titrate until the red color
changes to a clear blue and does not revert to red upon standing for 1 minute. Read
the test result from the scale where the large ring on the Titrator meets the Titrator
barrel. Multiply by 5.16. This is Titration Value B.
IV. FINAL RESULTS
Calcium Content = 0.4 x Titration Value B = ppm Ca
Magnesium Content = 0.24 (Value A - Value B) = ppm Mg
Multiply the results by 2 to obtain the content in pounds per acre.
EXAMPLE:
Titration Value A is 640 ppm CaCO
Titration Value B is 520 ppm CaCO
3
3
Calcium = 0.4 x 520 = 208 ppm Ca
= 208 x 2 = 416 lb/acre Ca
Magnesium = 0.24 (640 - 520)
= 0.24 x 120 = 29 ppm Mg
= 29 x 2 = 58 lb/acre Mg
Testing
Smart3 Soil Test Procedures 09.18 CALCIUM & MAGNESIUM
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 88
CHLORIDE
DIRECT READING TITRATOR METHOD • CODE M7241
QUANTITY CONTENTS CODE
15 mL *Chloride Reagent #1 *4504-E
60 mL *Silver Nitrate, 0.141N *3062DR-H
1 Test Tube, 5-10-15 mL, plastic, w/cap 0701
1 Spoon, 1g 0697
1 Test Tube, 5-10-15 mL, glass, w/cap 0778
1 Direct Reading Titrator, 0-1000 Range 0384
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Chlorides are present in practically all soils. Application of fertilizer may increase
chloride levels. Chlorides are removed from soil by leaching. Excessive concentrations
are toxic to plants. A high test reading, particularly where stunted growth has been
observed, may indicate poisoning due to high chloride levels in the soil. This test
is valuable on saline soils or when contamination from sea water or sea spray is
suspected. Normal soils of humid regions rarely give readable tests, except when
recently receiving liberal amounts of fertilizers containing chlorides.
Testing
RANGE: 0-1000 lb/acre Chloride
METHOD: In a neutral or slightly alkaline solution, potassium
dichromate indicates the endpoint of the silver nitrate
titration.
INTERFERENCES: Bromine, iodide and cyanide register as equivalent chloride
concentrations.
Smart3 Soil Test Procedures 9.18 CHLORIDE
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 89
PROCEDURE
Carefully read the LaMotte Direct Reading Titrator Manual (1649) before performing
the titration procedure described below. The Titrator is calibrated in terms of parts per
million chloride and each minor division on the Titrator scale equals 20 ppm.
1. Fill a clean test tube (0701) to the 15 mL line with deionized water.
Testing
2. Add 3 measures of soil using the 1 g spoon (0697). Cap tube and shake for fi ve
minutes.
3. Filter and collect all of the soil fi ltrate using the funnel (0459) and fi lter paper
(0463). The extract does not have to be clear since a slight turbidity does not
interfere in the test.
4. Fill the test tube (0778) to the 10 mL line with the fi ltrate.
5. Add three drops of *Chloride Reagent #1 (4504) to the sample. Cap and shake to
mix. A yellow color will result.
6. Fill the Direct Reading Titrator (0384) with *Silver Nitrate, 0.141 (3062DR) in the
manner described in the instruction manual.
7. Titrate the test sample with *Silver Nitrate, 0.141 (3062DR) until the yellow color
changes permanently to pink. Read the test result from the scale where the large
ring on the Titrator meets the Titrator barrel. If the plunger reaches the bottom
mark (1000 ppm) on the Titrator scale before the endpoint color change occurs,
refi ll the Titrator and continue the titration procedure. Be sure to include the value
of the original amount added (1000 ppm) when recording the fi nal result.
CHLORIDE Smart3 Soil Test Procedures 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 90
COPPER
DIETHYLDITHIOCARBAMATE METHOD • CODE M3639-46-65-SC
QUANTITY CONTENTS CODE
15 mL *Copper 1 *6446-E
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Like many other micronutrients, the amount of available copper varies considerably with
the type of soil. Well drained sandy soils are usually low in copper while heavily clay-type
soils contain an abundant supply of copper. Like manganese, copper may be unavailable
in soils that have a high organic make-up because it readily forms insoluble complexes
with organic compounds.
Generally from 0.2-25 lb/acre of copper is added to the soil to correct a defi cient level.
Copper is another metal that is necessary in the formation of the chlorophyll molecule
and like other metals, e.g. iron, manganese and zinc acts as a catalyst.
RANGE: 0.00–35.00 ppm Copper
METHOD: Copper ions form a yellow colored chelate with
diethyldithiocarbamate around pH 9-10 in proportion to
the concentration of copper in the sample.
INTERFERENCES: Bismuth, cobalt, mercurous, nickel and silver ions and
chlorine (6 ppm or greater) interfere and must be absent.
Testing
Smart3 Soil Test Procedures 9.18 COPPER
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 91
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Select All Tests (or another sequence containing 036 Cu Thiocarbamate) from
Testing
4. Scroll to and select 036 Cu Thiocarbamate from menu.
5. Fill a clean tube (0290) to the 10 mL line with the soil extract then neutralize
6. Insert tube into chamber, close lid and select Scan Blank.
7. Remove tube from colorimeter and add 5 drops of *Copper 1 (6446). Cap and mix.
8. Insert tube into chamber, close lid and select Scan Sample. Multiply the result by 5
ENTER
to select Testing Menu.
Testing Menu.
according to the procedure on page 84.
Solution will turn yellow if copper is present.
to determine the copper concentration in ppm.
9. Press to turn colorimeter off or press
EXIT
to exit to a previous menu or
make another menu selection.
NOTE: The reaction may stain the tubes. Scrub the tubes thoroughly after each use.
Copper Concentration Chart
Parts Per Million Range
0-1 ppm Low
1-3 ppm Marginal
3-4 ppm Adequate
COPPER Smart3 Soil Test Procedures 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 92
IRON
BIPYRIDYL METHOD • CODE 3648-SC
QUANTITY CONTENTS CODE
30 mL *Iron Reagent #1 *V-4450-G
5 g *Iron Reagent #2 Powder *V-4451-C
1 Pipet, 0.5 mL, plastic 0353
1 Spoon, 0.1 g, plastic 0699
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Iron is essential to the formation of chlorophyll, and iron defi ciency causes chlorosis.
While most soils contain abundant iron, only a fraction is soluble and readily available to
the growing plant. This is particularly true in neutral or alkaline soils. Acid soils contain
higher levels of available iron.
Testing
RANGE: 0.00–30.00 ppm Iron
METHOD: Ferric iron is reduced to ferrous iron and subsequently
forms a colored complex with bipyridyl for a quantitative
measure of total iron.
INTERFERENCES: Strong oxidizing agents interfere, as well as copper and
cobalt in excess of 5.0 mg/L.
Smart3 Soil Test Procedures 9.18 IRON
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 93
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Select All Tests (or another sequence containing 055 Iron Bipyridyl) from Testing
Testing
4. Scroll to and select 055 Iron Bipyridyl from menu.
5. Fill a clean tube (0290) to the 10 mL line with the soil extract then neutralize
6. Insert tube into chamber, close lid and select Scan Blank.
7. Remove tube from colorimeter. Use the 0.5 mL pipet (0353) to add one measure of
8. Use the 0.1 g spoon (0699) to add 0.1 g of *Iron Reagent #2 Powder (V-4451).
9. At the end of 3 minute waiting period, DO NOT MIX. Insert tube into chamber,
ENTER
to select Testing Menu.
Menu.
according to the procedure on page 84.
*Iron Reagent #1 (V-4450). Cap and mix.
Cap and shake vigorously for 30 seconds. Wait three minutes for maximum color
development.
close lid and select Scan Sample. Multiply results by 5 to determine the iron
concentration in ppm.
10. Press to turn colorimeter off or press
EXIT
to exit to a previous menu or
make another menu selection.
NOTE: For best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent
blank, follow the above test procedure to scan a distilled or deionized water blank. Then
follow the above procedure to perform the test on a distilled or deionized water sample.
This test result is the reagent blank. Subtract the reagent blank from all subsequent
test results of unknown samples. It is necessary to determine the reagent blank only
when a new lot number of reagents is obtained.
Iron Concentration Chart
Parts Per Million Range
0.0-1.3 ppm Very Low
1.4-3.0 ppm Low
3.0-5.0 ppm Medium
5.0-10.0 ppm Medium High
Over 10.0 ppm High
IRON Smart3 Soil Test Procedures 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 94
MANGANESE
PERIODATE METHOD • CODE 3669-SC
QUANTITY CONTENTS CODE
10 g Manganese Buff er Reagent 6310-D
15 g *Manganese Periodate Reagent *6311-E
1 Spoon, 0.1 g, plastic 0699
1 Spoon, 0.15 g, plastic 0727
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
The amount of manganese available to the plant is dependant upon the soil pH, the
quantity of organic matter present, and the degree of aeration. Manganese defi ciency
is most likely to occur in neutral or alkaline soils because it is less soluble at elevated
pH levels. In extremely acid soils, where manganese is more soluble, toxic levels may
exist which may reduce crop yields. In slightly acid sandy soils, manganese may leach
past the root zone and not be able for utilization by the plant. Also, it is believed that
manganese may form insoluble organic complexes in some soils that have high humus
content. All of the factors contribute to the availability of this essential element. Only
soil or tissue tests can determine whether defi cient or toxic levels of manganese exist.
Testing
Although manganese is known to play an important role in many of the metabolic
processes in the plant, little is known about its function other than it is required in some
enzyme reactions and is required for the formation of chlorophyll in the plant.
RANGE: 0.00–75.00 ppm Manganese
METHOD: Periodate oxidizes soluble manganous compounds into
permanganate.
INTERFERENCES: Reducing substances capable of reacting with periodate or
permanganate must be removed or destroyed before the
periodate oxidation is attempted.
Smart3 Soil Test Procedures 9.18 MANGANESE
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 95
PROCEDURE
1. Press and hold until colorimeter turns on.
2. Press
3. Select All Tests (or another sequence containing 059 Manganese HR) from Testing
Testing
4. Scroll to and select 059 Manganese HR from menu.
5. Fill a clean tube (0290) to the 10 mL line with the soil extract then neutralize
6. Insert tube into chamber, close lid and select Scan Blank.
7. Remove tube from colorimeter. Use the 0.1 g spoon (0699) to add two measures of
8. Use the 0.15 g spoon (0727) to add one measure of *Manganese Periodate
9. At the end of the two minute waiting period, mix, insert tube into chamber, close
ENTER
to select Testing Menu.
Menu.
according to the procedure on page 84.
Manganese Buff er Reagent (6310). Cap and mix until powder dissolves.
Reagent (6311). Cap and shake for one minute. An undissolved portion of the
reagent may remain in the bottom of the tube without adversely aff ecting the test
results. Wait two minutes for maximum color development. Solution will turn pink if
manganese is present.
lid and select Scan Sample. Multiply the result by 5 to determine the manganese
concentration in ppm.
10. Press to turn colorimeter off or press
EXIT
to exit to a previous menu or
make another menu selection.
Mangansese Concentration Chart
Parts Per Million Range
0-5 ppm Low
5-12 ppm Medium
13-24 ppm Medium High
25-40 ppm High
Over 40 Very High
MANGANESE Smart3 Soil Test Procedures 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 96
NITRATE-NITROGEN
CADMIUM REDUCTION METHOD • CODE 3649-SC
QUANTITY CONTENTS CODE
2 x 60 mL *Mixed Acid Reagent *V-6278-H
5 g *Nitrate Reducing Reagent *V-6279-C
1 Spoon, 0.1 g, plastic 0699
1 Dispenser Cap 0692
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Nitrogen is a component of the chlorophyll (green color) in plants, thus giving plants
the rich green color characteristic of a healthy plant. Nitrogen promotes succulence
in forage crops and leafy vegetables. When used at the recommended rates, nitrogen
improves the quality of leaf crops. It also simulates the utilization of phosphorus,
potassium and other essential nutrient elements. The above-ground growth of plants is
enhanced with nitrogen. Nitrogen hastens crop maturity (assuming all other nutrients
are adequately supplied and excessive nitrogen rates are not applied). Nitrogen is very
infl uential in fruit sizing.
Testing
RANGE: 0.00–300.00 lb/acre Nitrate Nitrogen
METHOD: Powdered cadmium is used to reduce nitrate to nitrite.
The nitrite that is originally present plus reduced nitrate
is determined by diazotization of sulfanilamide and nitrite
followed by coupling with N-(1 naphthyl)-ethylenediamine
dihydrochloride to form a highly colored azo dye which is
measured colorimetrically.
INTERFERENCES: Nitrite interferes at all levels. Strong oxidizing and reducing
substances interfere. Low results might be obtained for
samples that contain high concentrations of iron and
copper.
Smart3 Soil Test Procedures 9.18 NITRATE-NITROGEN
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 97
PROCEDURE
NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for
refi ll reagents.
1. Press and hold until colorimeter turns on.
Testing
2. Press
3. Select All Tests (or another sequence containing 065 Nitrate-N LR) from Testing
4. Scroll to and select 065 Nitrate-N LR from menu.
5. Use the 1 mL pipet (0354) to add 1 mL of soil extract to a clean tube (0290) and
6. Insert tube into chamber, close lid and select Scan Blank.
7. Remove tube from colorimeter and pour off 5 mL of the diluted extract into
8. Pour the 5mL diluted extract from a graduated cylinder or similar into the tube. Use
9. Use the 0.1 g spoon (0699) to add two measures of *Nitrate Reducing Reagent (V-
10. Hold tube by index fi nger and thumb and mix by inverting approximately 60 times a
ENTER
to select Testing Menu.
Menu.
dilute to the 10 mL line with deionized water. Cap tube and mix.
graduated cylinder or similar. Discard the remaining diluted extract.
the graduated cylinder or similar to measure 5 mL of *Mixed Acid Reagent (V-6278)
and add to tube. Cap and mix. Wait 2 minutes.
6279). Cap.
minute for four minutes. Wait 10 minutes for maximum color development.
NOTE: At end of waiting period an undissolved portion of Nitrate Reducing Reagent
may remain in bottom of the tube without aff ecting results.
11. At the end of the 10 minute waiting period, mix, insert tube into chamber, close
lid and select Scan Sample. Multiply the result by 100 to determine the nitratenitrogen concentration in lb/acre.
12. Press to turn colorimeter off or press
EXIT
to exit to a previous menu or
make another menu selection.
NOTE: For best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent
blank, follow the above test procedure to scan a distilled or deionized water blank. Then
follow the above procedure to perform the test on a distilled or deionized water sample.
This test result is the reagent blank. Subtract the reagent blank from all subsequent
test results of unknown samples. It is necessary to determine the reagent blank only
when a new lot number of reagents are obtained.
To convert Nitrate Nitrogen (NO3–N) results to ppm Nitrate (NO
NITRATE-NITROGEN Smart3 Soil Test Procedures 9.18
–
), multiply by 4.4.
3
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 98
Nitrate-Nitrogen Concentration Chart
Pounds Per Acre Range
0.0-9.0 lb/acre Low
11-29 lb/acre Medium
33-51 lb/acre Medium High
53-100 lb/acre High
Over 100 lb/acre Very High
Testing
Smart3 Soil Test Procedures 9.18 NITRATE-NITROGEN
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 99
NITRITE-NITROGEN
DIAZOTIZATION METHOD • CODE 3650-SC
QUANTITY CONTENTS CODE
2 x 60 mL *Mixed Acid Reagent *V-6278-H
5 g *Color Developing Reagent *V-6281-C
1 Spoon, 0.1 g, plastic 0699
1 Dispenser Cap 0692
*WARNING: Reagents marked with an * are considered to be potential health hazards.
To view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the
contents list or in the test procedures. Omit any letter that follows or precedes the four
digit code number. For example, if the code is 4450WT-H, search 4450. To obtain a
printed copy, contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Nitrites are formed as an intermediate step in the production of nitrate. Soils that are
well drained and aerated contain only small amounts of nitrite nitrogen. Excessive
nitrites, which are toxic to plants, may result from soil conditions unfavorable to the
formation of nitrate, such as inadequate aeration. High nitrite readings may also be
encountered in soils with large amounts of nitrates, where
a portion of the nitrate nitrogen decomposes to form nitrites.
Testing
RANGE: 0.00–40.00 lb/acre Nitrite-Nitrogen
METHOD: The compound formed by diazotization of sulfanilamide and
nitrite is coupled with N–(1–naphthyl)–ethylenediamine
to produce a reddish-purple color, which is read
colorimetrically.
INTERFERENCES: There are few known interfering substances at
concentration less than 1000 times the nitrite-nitrogen
concentration; however, the presence of strong oxidants or
reductants may readily aff ect nitrite concentrations. High
alkalinity (above 600 mg/L) will give low results due to a
shift in pH.
Smart3 Soil Test Procedures 9.18 NITRITE-NITROGEN
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Page 100
PROCEDURE
NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for
refi ll reagents.
1. Press and hold until colorimeter turns on.
Testing
2. Press
3. Select All Tests (or another sequence containing 068 Nitrite-N LR) from Testing
4. Scroll to and select 068 Nitrite-N LR from menu.
5. Use the 1 mL pipet (0354) to add 2 mL of soil extract to a clean tube (0290) and
6. Insert tube into chamber, close lid and select Scan Blank.
7. Remove tube from colorimeter and pour off 5 mL of the diluted extract into a
8. Pour the 5 mL diluted extract from the graduated cylinder into the colorimeter
9. Use the 0.1 g spoon (0699) to add two measures of *Color Developing Reagent (V-
ENTER
to select Testing Menu.
Menu.
dilute to the 10 mL line with deionized water. Cap tube and mix.
graduated cylinder or similar. Discard the remaining diluted extract.
tube. Use graduated cylinder or similar to measure 5 mL of *Mixed Acid Reagent
(V-6278) and add to tube. Cap and mix.
6281). Cap and mix by gently inverting for 1 minute. Wait 5 minutes for maximum
color development.
10. At the end of the 5 minute waiting period, mix, insert tube into chamber, close lid
and select Scan Sample. Multiply the result by 50 to determine the nitrite-nitrogen
concentration in lb/acre.
11. Press to turn colorimeter off or press
EXIT
button to exit to a previous
menu or make another menu selection.
NOTE: To convert nitrite-nitrogen (NO2–N) results to ppm nitrite (NO
by 3.3.
–
), multiply results
2
Nitrite-Nitrogen Concentration Chart
Pounds Per Acre Range
0.0-2.0 lb/acre Low
2.5-4.0 lb/acre Medium
4.5-10.0 lb/acre Medium High
Over 10 lb/acre High
NITRITE-NITROGEN Smart3 Soil Test Procedures 9.18
Find Quality Products Online at: sales@GlobalTestSupply.com
www.GlobalTestSupply.com
Loading...