PLANT TIS SUE TEST KIT
L
Mott
WARNING! This set contains chemicals
that may be harmful if misused. Read
cautions on individual containers
carefully. Not to be used by children
except under adult supervision
NI TRATES, PHOS PHO RUS, PO TAS SIUM
MODEL PT -3R • CODE 5026
QUANTITY CONTENTS CODE
2 x 120 mL *Universal Extracting Solution, Concentrated *5227-J
30 mL *Nitrate Reagent #1 *5146-G
30 g *Nitrate Reagent #2 Powder *5147-G
30 mL *Phosphorus Reagent #2 *5156-G
50 *Phosphorus Reagent #3 Tablets *5157-H
50 *Potassium Reagent B Tablets *5161-H
120 mL *Potassium Reagent C *5162-J
1 Funnel, plastic 0459
1 Filter Papers, (100 sheets) 0465
1 Test Tube, w/cap, “Potash A” 0245
1 Test Tube, “Phosphorus B” 0244
2 Test Tubes, extraction, w/caps, 7 & 14 mL 0704
1 Spot Plate, plastic 0159
1 Spoon, 0.5 g 0698
1 Stirring Rod, plastic 0519
2 Pipets, 1.0 mL, plastic 0354
1 Pipet, transfer, plastic 0364
1 Pipet, plain, glass, w/cap 0341
1 Pipet, plastic, w/cap 0392
1 LaMotte Soil Handbook 1504
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to
www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
To order individual reagents or test kit components, use the specified code number.
Distilled water should be used in the extraction procedure. Natural waters
may be used only if they are free of nitrates (i.e., a nitrate test is performed on
the water sample blank gives no result). Phosphorus and potassium, if present,
are usually present in trace amounts only.
COL LEC TION & PREP A RA TION
OF PLANT TIS SUE SAM PLES
Fresh plant material should be obtained from the growing crop, both from
normal and problem plants. It is important to test both healthy and problem
plants, as the tests are most meaningful when used in a comparative manner.
Select small lots of the leaf petioles, or succulent portion of the stem, in the
part of the plant most affected by any observable abnormal symptoms. Using a
clean, sharp knife or razor blade, cut the material into fine bits of not more
than g to
that will fill the Extraction Tube (0704) to the bottom mark when placed in
1
inch in length and thickness. An amount of material is used
16
the tube without packing.
EX TRAC TION PRO CE DURE
1. Fill the Extraction Tube (0704) to the lower line with the plant tissue
to be tested.
2. Use a 1 mL pipet (0354) to add 2 mL of *Universal Extracting
Solution, Concentrated (5227).
3. Fill the tube to the upper line with distilled or Nitrate-free water.
4. Cap and shake vigorously for 5 minutes.
5. Use a sheet of filter paper (0465) and the funnel (0459) to filter the
tissue extract into a clean Extraction Tube.
NI TRATE NI TRO GEN TEST
1. Use a clean 1 mL pipet (0354) to transfer 1 mL of the filtered tissue
extract to one of the larger depressions on the spot plate (0159).
2. Use the plastic pipet with screw cap (0392) to add 10 drops of
*Nitrate Reagent #1 (5146) to the filtrate in the spot plate.
3. Use the 0.5 g spoon (0698) to add 0.5 g of *Nitrate Reagent #2
Powder (5147). Stir thoroughly with a Stirring Rod (0519).
4. Allow to stand 5 minutes for full color development. Then observe
the color. Compare resulting color from the healthy plant test with
the problem plant test.
Results:
Dark Pink Color Abundant nitrate
Light Pink Color Adequate nitrate
No color No nitrate reserve, probably deficient
PHOS PHO RUS TEST
1. Fill the “Phosphorus B” Tube (0244) to the line with the filtered
tissue extract.
2. Use the glass pipet with screw cap (0341) to add 6 drops of
*Phosphorus Reagent #2 (5156) to the tube containing the filtrate.
3. Add one *Phosphorus Reagent #3 Tablet (5157). Cap and shake
until tablet dissolves.
4. Immediately note the color. Compare the color development from
healthy and problem plants.
Results:
Deep Blue Color Abundant phosphorus
Light Blue Color Adequate phosphorus
Yellow to Colorless Deficient to
Extremely Deficient phosphorus
PO TAS SIUM (POT ASH) TEST
1. Fill the “Potash A” Tube (0245) to the lower line with the filtered
tissue extract.
2. Add one *Potassium Reagent B Tablet (5161). Cap and shake until
tablet dissolves.
3. Use the transfer pipet (0364) to add *Potassium Reagent C (5162)
until the tube is filled to the upper line. Allow the *Potassium
Reagent C to run slowly down the side of the tube. Swirl tube gently
to mix. A precipitate indicates the presence of potassium. The
heavier the precipitate, the more potassium is present. Compare
formation of precipitate in healthy plant tests and problem plant
tests.
Results:
Heavy Precipitate Adequate to
Abundant potassium (potash)
Medium Precipitate Possible potassium deficiency
Trace Precipitate Deficient potassium
No Precipitate Extremely Deficient potassium
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Chal lenges
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
®
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