LaMotte POLYPHOSPHATE KIT LOW RANGE COMPARATOR User Manual

PLANT TIS SUE TEST KIT

Mott

WARNING! This set contains chemicals

that may be harmful if misused. Read

cautions on individual containers

carefully. Not to be used by children

except under adult supervision

NI TRATES, PHOS PHO RUS, PO TAS SIUM

MODEL PT -3R • CODE 5026

QUANTITY CONTENTS CODE

2 x 120 mL *Universal Extracting Solution, Concentrated *5227-J 30 mL *Nitrate Reagent #1 *5146-G 30 g *Nitrate Reagent #2 Powder *5147-G 30 mL *Phosphorus Reagent #2 *5156-G 50 *Phosphorus Reagent #3 Tablets *5157-H 50 *Potassium Reagent B Tablets *5161-H 120 mL *Potassium Reagent C *5162-J 1 Funnel, plastic 0459 1 Filter Papers, (100 sheets) 0465 1 Test Tube, w/cap, “Potash A” 0245 1 Test Tube, “Phosphorus B” 0244 2 Test Tubes, extraction, w/caps, 7 & 14 mL 0704 1 Spot Plate, plastic 0159 1 Spoon, 0.5 g 0698 1 Stirring Rod, plastic 0519 2 Pipets, 1.0 mL, plastic 0354 1 Pipet, transfer, plastic 0364 1 Pipet, plain, glass, w/cap 0341 1 Pipet, plastic, w/cap 0392 1 LaMotte Soil Handbook 1504

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.

To order individual reagents or test kit components, use the specified code number.

Distilled water should be used in the extraction procedure. Natural waters may be used only if they are free of nitrates (i.e., a nitrate test is performed on the water sample blank gives no result). Phosphorus and potassium, if present, are usually present in trace amounts only.

COL LEC TION & PREP A RA TION OF PLANT TIS SUE SAM PLES

Fresh plant material should be obtained from the growing crop, both from normal and problem plants. It is important to test both healthy and problem plants, as the tests are most meaningful when used in a comparative manner. Select small lots of the leaf petioles, or succulent portion of the stem, in the part of the plant most affected by any observable abnormal symptoms. Using a clean, sharp knife or razor blade, cut the material into fine bits of not more than g to that will fill the Extraction Tube (0704) to the bottom mark when placed in

inch in length and thickness. An amount of material is used

the tube without packing.

EX TRAC TION PRO CE DURE

1. Fill the Extraction Tube (0704) to the lower line with the plant tissue to be tested.

2. Use a 1 mL pipet (0354) to add 2 mL of *Universal Extracting Solution, Concentrated (5227).

3. Fill the tube to the upper line with distilled or Nitrate-free water.

4. Cap and shake vigorously for 5 minutes.

5. Use a sheet of filter paper (0465) and the funnel (0459) to filter the

tissue extract into a clean Extraction Tube.

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