LaMotte POL-H User Manual

LaMotte BioPaddles™ TECH DOCUMENT

Call: 800-344-3100

Email: tech@lamotte.com

BioPaddles™ Colony Identification App

SAB/SAB

Code 5551

USE:

Selective isolation of fungi (yeasts and molds) (SAB)

Side 1 & 2: Sabouraud Dextrose Agar (SAB) (colorless / slightly hazy)

APPLICATION

Sabouraud Dextrose Agar (SAB) is a modification of dextrose agar described by Sabouraud.1 SAB is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permit selectivity of fungi.2 Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics,3 in the mycological evaluation of food,4 and clinically to aid in the diagnosis of yeast and fungal infections.5

PADDLE AGARS

Sabouraud Dextrose Agar (SAB) – Enzymatic digest of casein and enzymatic digest of animal tissue provide the nitrogen and vitamin source required for organism growth in SAB. The high concentration of dextrose is included as an energy source. Agar and a proprietary polymer are the solidifying agents.

CULTURE CONTROLS

10-300 inoculum (CFU)

SAB Agar Aspergillus niger GROWTH Candida albicans GROWTH Escherichia coli INHIBITED Microsporum canis GROWTH Trichophyton mentagrophytes GROWTH

Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.

Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.

Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (eds.). 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance Association, Washington, D.C.

U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8thed. AOAC International, Gaithersburg, MD.

Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). Manual of clinical microbiology, 6th ed. American Society for Microbiology,

Washington, D.C.

For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory.

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LaMotte BioPaddles™ TECH DOCUMENT

Call: 800-344-3100

Email: tech@lamotte.com

STORAGE / EXPIRATION

Store tightly sealed BioPaddles™ in a cool, dry location. Shield from direct sunlight. Store BioPaddles™ at room temperature (65 - 77°F/18 - 25°C). Avoid sudden temperature changes. Temperature fluctuations may result in condensation settling at the bottom of the vial. This will not affect the culture properties, but could reduce the shelf-life or cause the agar to separate from the plastic paddle support. Do not refrigerate. Refrigeration may result in water condensation. Avoid freezing. If freezing occurs, thaw for 3-6 hours in the refrigerator (40°F; 4.4°C). Freezing can promote excess water loss and variation in media surface due to crystal formation.

Refer to Best Before End date (See: BBE stamped on vial). Discard if paddle agar appears oxidized and darker than the expected color or if contaminants appear. The expiration date is based on medium in an intact container that is stored as directed.

SAMPLING

Liquids: Twist to remove paddle from vial. Fill vial to 40 mL fill line with the liquid to be sampled. The 40 mL volume can be used to calculate Total Viable Count (TVC) and/or Total Colony Count (TCC). Replace paddle. Allow a contact time of 15 seconds. Remove the paddle. Empty the vial. Replace the paddle in the vial.

Surfaces: Twist to remove paddle from vial. Allow the paddle surface (10 cm2) to come into physical contact with the test surface. Recovery rate is about 50%. To ensure an accurate recovery, gently sweep (or touch) the paddle to cover a 20 cm2 area. Replace paddle in vial.

INCUBATION

Temperature Minimum Period Optimal Period

35°C (bacteria) 72 hours 5-7 days 20-25°C (fungi) 5 days 7 days

COLONY MEASURING

Each BioPaddles™ paddle has molded media attachment points that are 4mm in length (point-to-point). This feature provides a useful guidepost to estimating nearby colony size.

For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory.

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IDENTIFICATION

ORGANISM

PHYSIOLOGY

◆

Precision Test Strip

Available

GROWTH

COLONY

LaMotte BioPaddles™ TECH DOCUMENT

Call: 800-344-3100

Email: tech@lamotte.com

SAB

IMAGE

B. subtilis

E. coli

lternaria spp.

spergillus niger

• Lactose (-)

• Indole (-) ◆

• Oxidase (-) ◆

• Catalase (+) ◆

• Urease (+) ◆

• Gram (+) Rod

• Lactose (+)

• Indole (+) ◆

• Oxidase (-) ◆

• Catalase (+) ◆

• Urease (-) ◆

• Gram (-) Rod)

• Catalase (+) ◆

• Ascomycete

•

Catalase (+) ◆

•

Ascomycete

+++

PARTIAL COMPLETE INHIBITION

+++

• Translucent to dull, off-white; opaque

• Smooth to rough

• irregular / dendroid margins

to spreading

• 2-4mm

---

• Downy to woolly; flat

• Grayish, short, aerial hyphae

• later becomes greenish black

or olive brown with a light border

• 2-5++cm

• Granular

• White, w/ jet black fruiting

bodies w/ yellow/gray hyphae

• 2-5++cm

---

For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory.

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