Labnet MultiGene Mini Personal Thermal Cycler User Manual

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MultiGeneMini

ThermalCycler

TC020-24 /TC020-24-230V TC050-18 /TC050-18-230V

Operation Manual

Ver.1.2

Labnet International

CONTENTS

CHAPTER 1 SPECIFICATIONS ...................................................................................................... 1

1 Normal Operating Conditions .................................................................................................. 1

2 Transportation and Storage Conditions................................................................................... 1

3 Basic Specifications................................................................................................................. 1

4 Performance ............................................................................................................................ 2

5 Software Functions .................................................................................................................. 2

CHAPTER 2 Features ........................................................................................................................ 3

1 Description............................................................................................................................... 3

2 Keyboard Diagram................................................................................................................... 4

3 Key Functions .......................................................................................................................... 4

4 Inspection before Power-up..................................................................................................... 5

5 Power-on Procedures .............................................................................................................. 5

CHAPTER 3 OPERATION GUIDE..................................................................................................... 6

1 How to Run a Program ............................................................................................................ 6

1.1 How to edit a program ....................................................................................................... 7

1.2 How to Set System Parameters ...................................................................................... 12

CHAPTER 4 TROUBLE SHOOTING............................................................................................... 14

1 Failure Analysis and Processing Procedures........................................................................ 14

2 Block Error Messages ........................................................................................................... 15

APPENDIX 1 Wiring Diagrams .................................................................................................... 16

APPENDIX 2 Parameter Values.................................................................................................. 18

CHAPTER 1 SPECIFICATIONS

This chapter describes the MultiGene Mini Thermal Cycler’s operation, transportation and storage conditions, basic parameters, performance and functions.

1 Normal Operating Conditions

Ambient operating temperature: 10°C ∼ 30°C Relative humidity: ≤70%

Power supply: ~100-120V, 200W, 50/60Hz ~220-240V, 200W, 50/60Hz

Note:

Before powering on, please confirm that the above operating conditions are satisfied. The unit must be plugged into a grounded outlet.

2 Transportation and Storage Conditions

Ambient temperature: -20 ºC～+55 ºC Relative humidity: ≤80%

3 Basic Specifications

Model Parameters

TC050-18

TC020-24

Sample Capacity

Tube Volume (ml)

0.5

0.2

Dimensions (mm)

297×212×200（L×W×H）

Display Screen

320×240 Backlit LCD

Weight (kg)

3.2

250V 2.5A (Ф5×20mm)

Fuse

125V 5A (Ф5×20mm)

Computer

Interface

RS232

4 Specifications

Temperature range: 4ºC～99ºC Maximum Heating rate: ≥5.0ºC/sec(max)

Maximum Cooling rate: ≥4.0ºC/sec(max) Temperature Resolution ± 0.1°C Block Temperature accuracy: ≤±0.3ºC Temperature display accuracy: ≤±0.5ºC Block temperature uniformity: ≤±0.5ºC Heated lid work temperature range: 105ºC±5ºC

Note: These performance specifications can be met when the unit is operated in Ambient

temperature: 23±5ºC,humidity≤70% Temperature control accuracy and block temperature uniformity are tested at the following

temperature settings: 55ºC，72ºC，95ºC

Max. number of cycles: 99 Max. Segments within a cycle: 9 Max. temperature time: 99 min. 59 sec. Max. incubation and in situ time: 99 hour 59 min.

Program storage capacity: 100

5 Software Functions

File editing and saving File accessing, modifying and deleting Automatic file link Heating/cooling rate setting Automatic temperature and time increments/decrements during cycling Real-time display of data at each phase of program execution Pause Stop Auto-restart in case of power failure Audible alarm Run time estimation Remaining time estimate Date and time (year, month, day, hour, minute, second) display Audible alarm in the event of failure

Note:

The above software functions are listed for reference only. Labnet reserves the right to modify the software without notice.

CHAPTER 2 Features

This chapter covers the MultiGene Mini Thermal Cycler’s mechanics, the keyboard and each key’s function, and preparations before power-on.

1 Description

① Heated lid & Block

② LCD Display

③ Keyboard

④ RS232 interface

⑤ Power switch

⑥ Power connecter

⑦ Fuse Socket

Note:

1 The fuse specifications are as follows: 250V:2.5A / 125V:5A, Ø5×20mm. The fuse

should be replaced with one that meets these specifications.

2 Contact your distributor or Labnet’s service department if you need proper fuses.

2 Keyboard Diagram

3 Key Functions

“CE”，can be used to change “+, -” when editing temperature and time.

“Right/Left”，moves the cursor one position to the left or right during file parameter setting.

“Up/Down”，moving the cursor one position up or down during file parameter setting.

“Enter”，press it to accept the present parameter settings displayed on the screen.

“Function”, press any button to accept the current items displayed above the buttons.

“Numbers & Letters”, select the letter by pressing the key until your selection appears on the screen.

4 Inspection before Power-up

Before switching the power on, please ensure that:

1) Supply voltage matches the voltage rating label on the instrument. (refer to Chapter 2);

2) The plug has been inserted into the power socket;

5 Power-on Procedures

Turn on the power (i.e. turn the power switch to position ‘1’), the MultiGene Mini Thermal Cycler will beep 4 times, and the LCD will display ‘ ’. This means the device is self-testing, which takes about 1-2 minutes. After the self-test is complete, the main menu will be displayed, and the device is ready for the following operations, editing, accessing, modifying, or deleting a file.

CHAPTER 3 OPERATION GUIDE

There are eight menu selections for the MultiGene Mini Thermal Cycler. In this chapter, we will explain operation, for example, how to edit, access, modify, delete, or run a protocol file and how to set parameters.

Warning!

If an abnormal sound or display appears on start up or if a failure error during the Self Test mode, Turn off the power immediately and contact Labnet or your distributor

Note:

If the number of samples is less than the number of wells in the block, the sample tubes should be evenly distributed over the block, in order to ensure that the heated lid will evenly press on the tops of the sample tubes. This also ensures that both the block’s load and the temperature distribution to each tube are even.

Note:

Before closing the cover, turn the knob counter-clockwise according to

the knob diagram until there is a "click....." Please note that the knob

can be tightened by turning it clockwise, After closing the cover but before power on, turn the knob clockwise until you hear a click, then turn the knob one more turn. When the knob is turned clockwise or counter-clockwise, you will hear a "click" sound, To open pull the release latch with your finger; and turn the knob counter-clockwise.

1 How to Run a Program

Switch on the power, the MultiGene Mini Thermal Cycler will beep 4 times. The screen will display “ ”，and the instrument will carry on self test. The self test takes about 1~2 minutes. If the self test is successful, the screen will

display the main Menu.

On the left top corner of the main screen the Hot lid status is displayed.

The center of the screen displays Labnet, the software version number, block model, default file, default user, control mode and sample volume.

 Press “File” to enter file list

interface.

 Press “System” to enter system

parameter setting menu.

Hotlid=020C 2009-04-01 10:05:00

Labnet

MultiGene Mini

Version: 2001-1.0

Default User: Labnet Default File: 1 Module Select: 24*0.2 Control Mode: Block

File System Run n

Hotlid=020C 2009-03-24 10:05:00

Labnet

MultiGene Mini

Version: 2009-1.0 Default User: Labnet Default File: 1 Module Select: 24*0.2 Control Mode: Block

Back Run n

If the default user does not have a password, press “Run” to run the default file. You will be prompted to enter sample volume(if the unit is set for Tube Mode) in the pop up window. If the unit is set for block mode, the unit will begin running the default file.

If the default user does have a password, you will be prompted to enter it before you can run the default file.

 Password Prompt

Use the keypad to enter the password. Press “Back” to go back the main menu. Press “Accept” to confirm the password and enter file running interface or sample volume input window. If the password is incorrect, a dialog box will be displayed as below:

 If the current Control Mode is Block

mode, press “Accept” button to enter file running interface. If the current Control Mode is Tube mode, press “Accept” button, a dialog box will be displayed to input the sample volume.

Enter sample volume used. By using Tube mode, the temperature overshoot and time will be adjusted for different sample volumes.

 Press “Back” to go back the main menu.  Press “Run” to enter the file running menu.

1.1 How to Edit a Program

Press F1 “File” on the main screen to enter the file list menu. There are 3 files listed as shown in the image. When the flashing bar is in the User Column, select users with the up/down keys; The File Name Column will display the files of the selected

Hotlid=off 2009-04-01 10:05:00

Labnet

MultiGene Mini

Version: 2009-1.0 Default User: Labnet Default File: 1 Module Select: 24*0.2 Control Mode: Block

Back Acceptt

Enter password

Please enter sample volume:

020µl

Please enter sample volume:

020µl

Password error. Try again.

Enter password

user. Shift to the File Name Column, using the “right” arrow button, then select the user’s file using the up/down keys.

 Press “Edit” to edit the file

indicated by the cursor.

 Press “New File” to create a new

file.

 Press “Delete” and you will be

prompted “Do you want to delete the file? Please select ‘F3’ or

‘F4’”, Select（Delete） or （Back）.

 Press “Back” to go back to the

main menu.

 If the file has no password, press

“Edit” to enter file editing interface; If the file has a password, press “Edit”, a dialog will be displayed to indicate the user to enter the password.

Enter the password. Press “Accept” to confirm the password, and the system will enter the file editing menu; or press “Back” to go back to the file list interface.

 If the file has no password, press

“Run” to enter file run menu or enter the sample volume. If the file is password protected, press “Run”, a dialog box will be displayed to input the password. Press “Back” to go back the file list interface. Press “Accept” to confirm the password.

After pressing “Run” in the file list interface, and entering the password (If Password protected):

 If the current Control Mode is Block

mode, Press “Accept” to enter the file running interface; If the current Control Mode is Tube mode, press “Accept”, and a dialog box will be displayed to input sample volume.

Hotlid=off 2008-07-28 10:05:00

No.

User

File Name

Save Time

Jeff1*

AMP1

2007-07-28

Mike2

AMP2*

2007-07-28

Sue3

AMP3

2007-07-28

Back Accept t

Enter password

Please enter sample volume:

020µl

Enter password

Hotlid=off 2008-07-28 10:05:00

No.

User

File Name

Save Time

Jeff1*

Amp1

2008-07-28

Mike2

Amp2*

2008-07-28

Sue3

Amp3

2008-07-28

Edit New File Delete Back Run n

Hotlid=off 2008-07-28 10:05:00

No.

User

File Name

Save Time

Jeff1*

AMP1

2008-07-28

Mike2

AMP2*

2008-07-28

Sue3

AMP3

2008-07-28

Back Run n

Please enter sample volume:

020µl

File List Menu

Hotlid=020C 2008-11-01 10:05:00

100

Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0

Time 01:00 02:00 01:45 00:00

Seg. Temp(C) Time Ramp(C/s) +Temp +Time

1c 95.0 01:00 #.# +0.0 +0:00

2c 55.0 02:00 #.# +0.0 +0:00

3c 72.0 01:45 #.# +0.0 +0:00

4 04.0 00:00 #.# +0.0 +0:00

Cycle1：×10 From 01 to 03

+Seg. +Cycler Delete Back Save/Run

Use the key pad to input the sample volume. Press “Back” to go back to the file list menu. Press “Run” to enter file running menu.

Note：

The symbol “*”at the top of User and File indicates the default User and file. The Default User name is LABNET and the default file is 1. The

default file cannot be renamed, but it can be edited.

File Editing Screen

In this screen, you can add and edit cycles and segments of the file. A File is comprised by cycles and segments. The cycles contain the segments, which are repeated. Move the cursor by pressing “Up/Down” and “Right /Left”. Press the keys to modify parameter settings. Press “CE” to change +/-.  Press “+Seg.” to add a segment.

In every segment, you can set temperature (Temp), duration (Time), Ramping rate (Ramp), temperature increment of every cycle (+Temp) and time increment of every cycle (+Time). Press “Delete” to delete the present segment.

NOTE: setting ramp to #.# provides the maximum ramping rate.

 Press “+Cycle” to set cycle number and choose cycle segments to be included

in the cycle. The figure shows a cycle including the first segment to the third one, and the cycle is set to repeat 10 times (x10).

 Press “+Cycle” repeatedly to add cycles (max.: 5 sections ). Press “Delete” to

delete the present segment. Move cursor by pressing “Up/Down” to enter the former segment or the latter.

 Press “Save” to enter the file save menu.  Press “Back” to go back to the File List menu.

Please Note:

1. After editing a program, save it first before running.

2. When setting “Time” as “--:--” (i.e. ∞), the hot lid will automatically turn off

when the block reaches the set temperature.

File Saving Screen

In this screen use the keypad to enter the file name. Press “Enter” to move the cursor down one position. Select User and File Name through the popup character selection box. Click left button to delete characters while clicking right button to move the cursor. Press the “Caps” key to shift from a capital to lowercase letter, and on the top left a capital or small letter indication will appear. (example: abc)

 Press “Main” to go back to the

main interface.

 Press “Save” to save the file. When the file is normally saved, “File has been

saved. Please continue another operation” will appear. Press “Enter” to close the reminder window. If the file name was already in use, the system will display（Rename）or continue to save (Save).

 If the current Control Mode is Block mode, press “Run” to enter the file running

interface; if the current Control Mode is Tube mode, pressing “Run” will open, a dialog box to input the sample volume.

Relation of cycle and segments in a file

File

section 1

Cycle1 ×** From ** to **

Seg. 1

Seg. 2

Seg. 16

section 2

Cycle2 ×** From ** to **

section 5

Cycle5 ×** From ** to **

File Save Interface

Hotlid=off 2009-07-28 10:05:00

abc

User： LabnetI I

File Name： 1 1

Password： ***** *

Password confirm：***** *

Main Caps Save Run n

Please enter sample volume: 050µl

Enter the sample volume. Press “Back” to go back to the file save menu. Press “Run” to start running the file.

In the file save menu, if you press “Run” without pressing “Save”, the system will display “The modifications will be lost after running. Do you still want to run?”

 Press “No” to go back file save

interface.

 Press “Yes” to enter the file

running menu and sample volume input screen. (Note: the modification here cannot be saved after the program is run.

When the file is running, “Now

Running•••” will continuously flash.

When the protocol is complete, “File running is over is displayed, Press ‘Enter’ to return to the main menu.”  Press “Stop” and “Do you want

to stop running?” will be displayed. Select ‘F1’or ‘F3’”.You can then select either “Stop” or “Start”.

 Press “Pause” and the display

will show “Pause state now. Press ‘F3’ to continue running.” You can then select “Start” to continue.

 Press “View File” to view the file in the editing format.

Note: the file cannot be edited while running

 Press “Skip” to stop current temperature segment and run the next segment.

Hotlid=off 2007-07-28 10:05:00

abc

User： Labnet i

File Name： Num1 1

Password： Num1 1

Password confirm：Num1 1

No Yes s

File running interface

Hotlid=off 2007-11-1 10:05:00

100

75 50 25

Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0

Time 1:00 02:00 01:45 00:00

File Name:Num1 User: Labnet Now Running ••• 1c Temp=00.0 Time=00:00 Cycle 01 of 10 Total Time: 00h 56m Remaining Time:00h 56m

Stop Pause Start Skip View File

1.2 How to Set System Parameters

In the main screen press “System” to enter the system parameter settings menu. Press “Enter” to move the cursor to the next item. This operation is followed by the symbol “ ”. Press “Up/Down” to scroll to an option, and information can be entered by using the keyboard. Press “Right/Left” to move cursor’s position.  Press “Back” to go back to the

main menu.

 Press “Save” to save parameters.

Note: When setting a parameter, press the “Save” button, otherwise the changes will be lost.

1.2.1 “Date” and “Time” indicate the system time and date.

1.2.2 “Module Select” stands for module type, including two optional modes,

named “18*0.5” and “24*0.2”. The Module type will be preset for the type of unit you purchased. If a different size block is later installed, this setting will need to be changed.

1.2.3 “Control Mode” stands for default control model after power-on,

including two optional modes, named “Block” and “Tube”.

“Block” mode offers basic temperature control during the course of cooling &

heating only. Compared to the block, the cooling & heating rate of the samples in the block are a little slower, therefore, the actual rate of change of the sample temperature is different from the block temperature. The actual time for the sample to reach the block temperature is longer than the time it takes for the block to reach the set temperature. Therefore the dwell time of the sample at the set temperature can be shorter than the dwell time of the block at the set temperature.(Please refer to the following figure: relation between block temperature and reagent temperature.)

System Parameter Setting

Block Reagent

Hotlid=off 2009-02-28 10:06:00

Date(yy-mm-dd): 09-02-28 Time(hh-mm-ss): 10:06:19

Module Select: 24*0.2 Control Mode: Block Hotlid Temp: OFF Block Temp: 10C

Key Sound: OFF Run End Sound: ON

Back Save 5

“Tube mode” controls the cooling & heating rate of the block, inconsideration of the sample temperature changing at a slower rate. In tube mode, there is an overshoot after reaching the set temperature, which allows the sample to reach the set temperature faster, and will help the stay at the set temperature for a longer period. (Please refer to the following figure: relation between block temperature and reagent temperature.)

1.2.4 “Key Sound” and “Run End Sound” stand for an audible key tone when

pressing a key and an audible alert at the end of a run, respectively.

Block

Reagent

CHAPTER 4 TROUBLE SHOOTING

1 Failure Analysis and Processing Procedures

No.

Phenomenon

Failure Analysis

Troubleshooting

Disconnected power supply

Check power supply for correct connection

Bad fuse

Replace it (250V 2.5A,Φ5×20mm or 125V 5A, Φ5×20mm )

Switch failure

Replace switch

No display and the two beeps missed when power on

Others