Labnet Labpette R Instruction Manual

1. Components and functions

(1) Plunger

Depressing the plunger dispenses the set volume of liquid

(2) Volume adjustment dial

Select position 1 to 5 to set desired volume (see table 1)

(3) Fill lever

Fills tip with liquid when pushed upwards

(4) Locking lever

Secures reservoir tip in place

(5) Reservoir tip

Reservoir for holding liquid to be dispensed. For best
results use Labnet Combi-Syringes.

Labpette R

Instruction Manual

2. Selecting the volume

• Locate the volume to be dispensed in table 1

• Set the volume adjustment dial to the position

indicated at the top of the column.

• Choose the appropriate reservoir tip from the left
hand column of the chart.

• The maximum number of dispensing steps for the
selected tip and volume is located at the bottom of
the column.

3. Inserting reservoir tip

• Push the fill lever down until it stops.

• Push the locking lever upwards.

• Insert a reservoir tip with slight pressure to lock it in

place.

• Push the fill lever down slightly and press down on
the locking lever to secure the tip in place.

• Use the volume adjustment dial to set the required
volume.

4. Filling the reservoir tip

• Immerse the end of the reservoir tip into the liquid to
be drawn up.

• Slowly raise the fill lever upwards to draw liquid into
the tip.

• Dispense the first step with the plunger.

Table 1
Dial position 12345

using 0.05 ml tip 1 µl2 µl3 µl4 µl5 µl
using 0.50 ml tip 10 µl 20 µl 30 µl40 µl50 µl
using 1.25 ml tip 25 µl 50 µl 75 µl 100 µl 125 µl
using 2.50 ml tip 50 µl 100 µl 150 µl 200 µl 250 µl
using 5.00 ml tip 100 µl 200 µl 300 µl 400 µl 500 µl
using 12.50 ml tip 250 µl 500 µl 750 µl 1000 µl 1250 µl
using 25.00 ml tip 500 µl 1000 µl 1500 µl 2000 µl 2500 µl
using 50.00 ml tip 1000 µl 2000 µl 3000 µl 4000 µl 5000 µl
Steps 48 23 15 1 1 8

Please note :

25 ml und 50 ml tips require the use of an
adapter (adapters are supplied free with
each Labpette R repeating pipettors).

Attach the adapter by placing it on the tip

as shown in the illustration and turning
clockwise. After the tip has been used,
the adapter may be removed and reused.

0,05 ml tips should be used with a 10
pipette tip on the liquid end.

The fill lever must be raised slowly to
prevent negative pressure and air

bubbles in the liquid.
A small air bubble in the upper part of
the reservoir will not affect the
accuracy as the last step is not
carried out.

µl

The reservoir tip does not need to be
filled completely.

The 0.5 ml, l, 1.25 ml and 2.5 ml tips
may be used with a 100

affixed to the end. This facilitates filling
from vessels with narrow necks and
provides better accuracy with small
volumes.

µl pipette tip

5. Dispensing

• Check that the volume setting is correct.

• Wipe the end of the tip with a lint free wipe or other

appropriate material.

• Place the tip at an angle to the wall of the vessel.
Press the plunger down to the stop to dispense.

After using the 25 ml and 50 ml
tips, the adapter can be removed
by turning it counter-clockwise and
reused with another tip.

Avoid touching the end of the tip

when removing it from the pipette.
When using viscous solutions,
the fit of the tip may loosen after
several pipettings.

6. Removing the tip

• Empty the tip completely.

• Push the locking lever upwards.

• Remove the tip from the dispenser.

Do not attempt to disassemble the
Labpette R repeating pipettor. Doing so will void
the warranty.

7. Maintenance

Labpette R repeating pipettors requires no
maintenance. Potential clogs are best removed
with warm water or isopropanol.

8.
Troubleshooting

Problem Possible cause Remedy

Tips will not fit in pipette Fill lever has not been pushed down completely Push fill lever down to the
stop

Locking lever cannot be pushed down Tip has not been inserted Properly Remove the tip and push
tip piston in. Be sure the
filling lever is in the down
position, replace tip and
press locking lever down
again

Fill lever cannot be moved upwards Locking lever has not been pushed Push the locking lever
down completely down to the stop

Dispensed volume is not correct Volume adjustment wheel is not locked in place Lock adjustment wheel in
place

Table 2

9. Technical data

Weight : Approx. 100 grams
Accuracy and precision : see Table 2
Liquid : bidistilled water
Reference temperature : 20° - 25° C

± 0.5° C constant

Measurement : according to

Subject to technical modifications
Made in Germany

DIN 12650

Dial position 1 2 3 4 5 Inpre- Inaccur­Steps 48 23 15 11 8 cision (%) acy(%)

using 0.05 ml tip 1
using 0.50 ml tip 10
using 1.25 ml tip 25
using 2.50 ml tip 50
using 5.00 ml tip 100
using 12.50 ml tip 250
using 25.00 ml tip 500
using 50.00 ml tip 1000

10.Ordering information

Labnet Combi-Syringe tips may be ordered
from your Labnet dealer. All sizes are supplied
in stackable dispenser boxes and are available
in sterile or non-sterile packaging.

11.Warranty

Labpette R repeating pipettors are guaranteed
against defects in materials and workmanship
for a period of 6 months from the data of
purchase. This warranty is valid only if the
dispenser has been used in compliance with
these instructions. If a failure cannot be
remedied using the troubleshooting guide
above, contact Labnet for further assistance.

µl2 µl3 µl4 µl5 µl< 5 < ± 2,5

µl 20 µl 30 µl 40 µl 50 µl < 0,7 < ± 0,8
µl 50 µl 75 µl 100 µl 125 µl < 0,5 < ± 0,8
µl 100 µl 150 µl 200 µl 250 µl < 0,4 < ± 0,8

µl 200 µl 300 µl 400 µl 500 µl < 0,3 < ± 0,4
µl 500 µl 750 µl 1000 µl 1250 µl < 0,3 < ± 0,3
µl 1000 µl 1500 µl 2000 µl 2500 µl < 0,3 < ± 0,2

µl 2000 µl 3000 µl 4000 µl 5000 µl < 0,2 < ± 0,2

P.O. Box 841 • Woodbridge, NJ 07095 • Phone: 888-LABNET1 or 732 417-0700
Fax: [phone] • email: [e-mail] • http://www.labnetlink.com