Labnet ENDURO GEL XL E0160, ENDURO GEL XL E0160-230V, ENDURO GEL XL E0160-230V-UK, ENDURO GEL XL E160-CAN User Manual
E0160
ENDURO™ GEL XL
User Manual
HORIZONTAL GEL ELECTROPHORESIS UNIT
Lit M00078 Rev 3; September 2016
E0160-230V
E0160-230V-UK
E160-CAN
About This Manual
This manual is designed to assist you in the optimal usage of your Enduro
GelXL. The manual is available in English, French, German, Italian, Portuguese,
and Spanish on our website at www.labnetinternational.com/document-center
A.
TABLE OF CONTENTS
I. MAINTENANCE 1
II. OPTIONS AND SPECIFICATIONS 2
A.
Components and Accessories 2
Specifications 3
B.
III. OPERATING INSTRUCTIONS 3
A.
Preparation of the Agarose Gel and Electrophoresis Buffer – DNA 3
B. Preparation of the Agarose Gel and Electrophoresis Buffer - RNA 5
Casting the Gel 6
C.
D. Removing the Comb 7
E. Loading the Samples onto the Gel 8
F.
Electrical Connections to the Safety Lid 8
G. Sample Electrophoresis 9
Detection and Documentation of Separated Fragments 10
H.
IV APPENDICES 11
Buffers for Electrophoresis 11
B. Physical Properties of Electrophoretic Plastics 12
V REFERENCES 12
DECLARATION OF CONFORMITY 17
WARRANTY 18
I. MAINTENANCE
Please handle the unit with care:
Do not expose the unit or its accessories to temperatures above
60°C.
Do not expose the unit to organic solvents.
Do not clean the unit with abrasive cleaners or cleaning aids.
In most cases, rinsing with deionized water will sufficiently clean the unit.
For heavier dirt, use a mild cleansing solution such as dish soap (alkaline
cleansers are not recommended). Hand wash and dry with a soft cloth.
To remove residual ethidium bromide, occasionally soak the unit in 1%
commercial bleach solution for 16 hours. Rinse well.
PLEASE NOTE: The degradation of acrylic due to solvents may result in
substantial discoloration, cracking, warpage, or etching of the
electrophoresis unit.
Do not apply any of the following solvents: benzene, xylene,
toluene, chloroform, carbon tetrachloride, alcohols, phenols, ketones,
or esters.
Do not expose the ABS combs supplied with this unit to
formaldehyde for extended periods. When casting gels containing
formaldehyde, remove the combs promptly upon hardening of the gel
and rinse completely with deionized water.
Elimination of RNase Contamination
Should treatment of the unit to eliminate RNase contamination be desired, clean
the unit with a mild detergent as described above followed by soaking for 10
minutes in a solution of 3 % hydrogen peroxide, and then for 1 hour in 0.1 % DEPC
(diethyl pyrocarbonate). Pour out final rinse, and air dry.
CAUTION: DEPC is a suspected carcinogen; handle with care.
Alternatively, soak the unit and accessories in freshly made 2.2 mM acetic
anhydride treated water (200 µl/liter) for at least five minutes. Solutions for RNA
work (electrophoresis buffers, etc) may be made from the same acetic anhydride
treated water as well.
1
Catalog #
Description
E0161
E0162
E0163
E0164
E0165
(
2) 1mm 5/8 tooth Reversible com b
E0166
E0167
E0168
R1000
-
100BP
R1000
-
1KB
WARNINGS:
CAUTION! Injury, damage to equipment, or property, may result if used in a
manner not specified by the manufacturer.
CAUTION! A pi nch hazard exists between the plastic enclosure and the shaking
head.
CAUTION! NOT for use with f lam mable liquids.
II. OPTIONS AND SPECIFICATIONS
A. Components and Accessories
Catalog # Description
E0 160 ENDURO Gel XL Complete Electrophoresis System
Comes complete with 1) 12.5 x 12 cm, 2) 12.5 x 6 cm UV Transmittant casting
trays, casting stand with divider, and four 1.0 mm thick 28/14 reversible tooth
combs, power cord and manual.
Accessories
(1)12.5 x 12cm UV Transmittant Casting Tray
(2) 12.5 x 6 cm UV Transmittant Casting Tray
(4) 6 x 6cm UV Transmittant Casting Tray
(2) 1 mm x 14/28 tooth Reversible Comb
Micro casting set - (4) 6 x 6cm UV Transmittant Casting Tray, 2) 1mm
5/8 tooth Reversi ble combs, Casting stand with divider
Casting Stand with divider
Standard casting set – (1) 12.5 x 12cm tray, (2) 12.5x6cm trays, (4)
14/28 tooth multi-channel compatible combs, casting stand with
divider
Molecular Weight marker 100 bp
Molecular Weight marker 1 Kb
B. Specifications
Unit dimensions 24.5 x 17.0 x 6.2 cm
Gel dimensions 12.5 x 12.0 cm
Maximum sample capacity: 112 samples (4 combs, 26
samples each)
Buffer Capacity: 300 ml
Distance Between Electrodes: 13.5 cm
Electrophoresis Tank
Overall dimension 18.3 × 16.4 × 5.6 cm
Material characteristic UV transmitting (50% at 254nm,
80% at 312nm)
Solution volume 300ml (includes buffer and gels)
Safety Lid
Overall dimension 19.7 × 16.9 × 3.8 cm
Material characteristic UV non-transmitting Polycarbonate
2
Power Supply
Overall dimension 7.5 × 17.0 × 6.2 cm
Weight 410 g
Input Voltage AC100 - 240V, 50/60Hz
Output Voltage 10 to 150 volts; Constant peak
voltage of 150V
Output Amperage 10 to 400 mA
Maximum Wattage 45 W
Timer 99 hours 59 min, and continuous
model
Safety Switch Micro-sensor (hall) in the Power
Supply. No output without safety lid,
Memory Function Automatic memory (the last used V
and T)
III. OPERATING INSTRUCTIONS
A. Preparation of the Agarose Gel and Electrophoresis Buffer -
DNA
1. Select the percentage gel necessary to effectively resolve your
sample, using Table 1 as a guideline.
Table 1: Gel Concentrations and Resolving Ranges
Concentration of Efficient Range of
Agarose in Gel Separation of Linear DNA
(% w/V) (Kb)
0.3% 5 - 60
0.6% 1 - 20
0.7% 0.8 - 10
0.9% 0.5 - 7
1.2% 0.4 - 6
1.5% 0.2 - 3
2.0% 0.1 - 2
Table taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 1, 6.8 613.
2. Weigh an appropriate quantity of agarose (0.3 % means 0.3 g of
agarose per 100 ml of gel volume) and place it into a 250 ml flask.
Note a 4mm gel will use 100 mls of agarose solution.
3. Make 500 ml of either 1X TAE or 1X TBE electrophoresis buffer
(see below).
Electrophoresis Buffers
The two most commonly used buffers for horizontal
electrophoresis of double stranded DNA in agarose gels are TrisAcetate-EDTA (TAE) and Tris-Borate-EDTA (TBE). While the
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