Labnet Deluxe ProBlot Hybridization Systems User Manual

ProBlot

Hybridization Systems

Models 6, 12, 12S and 24

Instruction Manual

Copyright March 2004

PO Box 841 Woodbridge, NJ 07095 USA
Phone: [phone] Fax: [phone]

www.labnetlink.com

CONTENTS OF THIS USERS MANUAL

Graphic Symbols page 1 Operating Features page 4
Safety Precautions page 1 Shaker (Problot 12S only) page 5
Introduction page 2 Rocking Platform / Installation page 5
Specifications page 2 Care and Maintenance page 6
Unpacking page 2 Decontamination page 6
Installation page 3 Troubleshooting Guide page 6

Stacking Problot Ovens page 3 Technical Support / Service page 7
Controls & Calibration page 3 Accessories page 7

GRAPHIC SYMBOLS

Your Labnet Problot hybridization incubator uses internationally accepted graphic symbols
to help convey information to the user and to call the users attention to important safety
precautions and guides for using this equipment. Use of this product in any manner not

specified by the manufacturer may impair the safety protection provided by the
equipment and may result in physical damage and/or personal injury. Please read
all operating instructions in this manual prior to use of this equipment.

indicates that user should consult manual further description or discussion.

Indicates AC “Power On” Indicates “Heating”

Indicates “Degrees Centigrade” Indicates “Temperature”

Indicates Potential “Shock Hazard” Indicates Protective “Earthground”

SAFETY PRECAUTIONS

1. Although the Problot Hybridization system has been designed to minimize user expo­sure to radioactive materials, proper precautions must be taken when using radioactive
material.

2. Always inspect the hybridization bottles, caps and seals before use. Do not use
bottles, caps or seals that are cracked or chipped. Cap seals should be replaced if they
are severely deformed or discolored. Special attention should be paid to inspecting
bottle rims / threads.

3. DO NOT take bottles to temperatures higher than 70C without opening and retight­ening

the cap to relieve pressure build-up. Temperatures above 70C can cause breakage of
hybridization bottles. If chamber temperatures rise above 70C when bottles are in use,
DO NOT OPEN THE OVEN DOOR. Reduce the temperature setting to below 70C or
turn the power off and allow sufficient time for the bottles to cool below 70C before
opening the door.

INTRODUCTION

This manual covers the specifications, operation and use of the Labnet series of Problot Hybrid­ization Ovens including Problot 6, Problot 12, Problot 12S and Problot 24. Please pay special
attention to the

Safety Precautions

section in this manual.

Problot Hybridization Ovens from Labnet International provide extremely stable temperature
environments and smooth variable speed rotation that are ideal for blot hybridization and blot
washing activities.

Labnet’s Problot Hybridization Ovens use microprocessor controls and mechanical convection
to maintain a stable temperature environment and to achieve fast chamber temperature recov­ery after a door opening. The oven interior is constructed from stainless steel for corrosion
resistance and the oven includes a drip pan for easy clean-up of accidental spills.
Problot rotisseries are easily removed from the oven and are adjustable for either horizontal or
more vigorous angled blot washing. All oven doors have an integral glass window to allow
observation of samples without opening the door and each door is fully thermal gasketed.

An optional rocking platform is available for each oven and the Problot Model 12S includes an
integral orbital shaking platform (10mm orbit) with optional flask platform.

Labnet Hybridization Bottles are recommended for optimum Problot Oven performance. These
bottles are made of high quality borosilicate glass, which provides a high level of protection
from the 32P beta emissions.

SPECIFICATIONS

Chamber temperature range Ambient +5C to 80C
Accuracy / Uniformity +/-0.1C / +/-0.5C
Rotisserie Speed 4 to 20 rpm
Optional rocker speed 8 to 40 rocks per minute
Shaker speed (Problot 12S only) 10 to 300 rpm at a maximum load of 4kg, 10mm orbit
Shaker timer (Problot 12S only) Continuous (RUN) or 0 to 120 minutes timed
Interior electrical outlet 1 amp (on 120V Problot 6, 12 and 24 models only)

Electrical Ratings and Unit Size Exterior (WxDxH) Chamber (WxDxH)
Problot 6&12 120V, 50/60Hz, 6 amps 483x445x495mm 368x279x343mm

220V, 50/60Hz, 3 amps

Problot 12S 120V, 50/60Hz, 6 amps 483x445x495mm 368x279x343mm

220V, 50/60Hz, 3 amps

Problot 24 120V, 50/60Hz, 7 amps 627x422x826mm 2 x 368x279x343mm

UNPACKING

Upon receipt of your Problot Hybridization Oven, examine the carton and unit for damages. If shipping
damage has occurred, a claim must be filed with the carrier. The carrier is responsible