Labnet C0336, C0336R User Manual
Centrifuge User Manual
C0336
C0336R
Copyright 2017
This manual was prepared with special care.
LABNET INTERNATIONAL may change the manual at any time and without notice because
of improvements, typographical errors, inaccuracies of current information or improvements
to facilities.
Content
page
1 Application ..........................................................................................1
2 Technical specification
3 Installation
3.1 Content of the package ...................................................................... 2
3.2 Location ......................................................................................2
3.3 Current protection ............................................................................3
..........................................................................................2
..........................................................................1
4 Safety notes ........................................................................................3
4.1 Operating personnel .......................................................................... 3
4.2 Warranty ......................................................................................3
4.3 Loading the rotor .............................................................................4
4.4 Safety hints ...................................................................................4
4.5 Maintenance conditions ......................................................................5
4.6 Safety precautions ............................................................................ 5
4.7 Residual risk ..................................................................................6
5 Operating ............................................................................................6
5.1 Centrifuge description ........................................................................6
5.2 Centrifuge overview ..........................................................................6
5.3 Construction ..................................................................................6
5.4 Rotor and accessories installation ........................................................... 7
5.5 Control device ................................................................................7
5.6 Setting parameters ...........................................................................7
5.7 Safety features................................................................................8
5.8 Increase in temperature (C0336 only) ....................................................... 8
6 Centrifuging ........................................................................................8
6.1 Control panel .................................................................................8
6.2 Display ........................................................................................9
6.3 Centrifuging notes .......................................................................... 10
6.4 Setting up RPM, RCF, TIME, temperature .................................................11
6.5 User programs .............................................................................. 12
6.6 Programs with user characteristics ....................................................... 15
6.7 Choosing rotors ............................................................................ 15
6.8 SHORT mode ............................................................................... 16
6.9 Terminating centrifugation .................................................................. 16
7 Temperature control ............................................................................ 16
7.1 Initial cooling during centrifuging –FAST COOL .......................................... 17
7.2 Initial cooling– THERMAL CHAMBER ..................................................... 17
7.3 Cooling in “START DELAY – OF TEMPERATURE” mode ............................... 17
7.4 Cooling in „SHORT” mode ................................................................. 17
7.5 Cooling notes ............................................................................... 17
8 Parameters of centrifugation ................................................................. 18
8.1 Accelerating/decelerating – changing characteristics .................................... 18
8.2 Radius ....................................................................................... 18
8.3 Sample density ............................................................................. 18
8.4 Temperature offset ......................................................................... 19
8.5 Thermal chamber ........................................................................... 19
8.6 Automatic lid opening ...................................................................... 20
8.7 Start delay - of time ......................................................................... 20
8.8 Start delay – of temperature ............................................................... 20
8.9 Errors ........................................................................................ 21
8.10 Temporarily disabled functions ............................................................. 22
8.11 Unbalance .................................................................................. 22
8.12 Screen saver ................................................................................ 23
8.13 Visual alarm ................................................................................. 23
8.14 Types of main screen ....................................................................... 23
8.14.1 Switching the basic display to simplified screen .......................................... 23
8.14.2 Switching the simplified screen to basic display .......................................... 23
8.15 Rotating time ................................................................................ 24
8.16 Sounds ...................................................................................... 24
8.17 Time/date ................................................................................... 24
8.18 Language choosing ........................................................................ 25
8.19 Other ........................................................................................ 25
8.20 Password protection ........................................................................ 26
8.21 Total work time ............................................................................. 27
8.22 Diagnostics ................................................................................. 27
8.23 Factory settings ............................................................................ 27
8.24 Rotor runtime .............................................................................. 27
8.25 Cycles history .............................................................................. 27
8.26 Manufacturer’s details ..................................................................... 27
9 Maintenance ...................................................................................... 28
9.1 Cleaning of the centrifuge .................................................................. 28
9.2 Maintenance of centrifuge elements ....................................................... 28
9.3 Sterilization ................................................................................. 29
9.3.1 Autoclaving ................................................................................. 29
9.4 Chemical resistance ........................................................................ 30
10 Troubleshooting ................................................................................. 31
10.1 Emergency cover release .................................................................. 32
Appendix A
Equipment Disposal - European Regulations ................................................ 32
Symbols and Conventions
Limited Warranty
NOMOGRAM
.................................................................................... 33
......................................................................................... 34
........................................................................ 32
1 Application
Centrifuges are used for separation samples taken from people’s, animal’s and plant’s components of different densities, under the influence of the centrifugal force, to provide information about their biological state (C0336 – ventilated,
C0336R– with cooling).
Its construction ensures easy operation, safe work and wide range of applications at laboratories engaged in routine
medical analyses, biochemical research works etc.
This centrifuge is not biotight and therefore during centrifugation of preparations requiring biotightness one has to
use closed and sealed containers and rotors. In the centrifuge, it is prohibited to centrifuge caustic, inflammable and
explosive preparations.
2 Technical specification
manufacturer LABNET INTERNATIONAL
type C0336 C0336R
mains voltage (L1+N+PE) 230V 120V 230V 120V
10% ±5% 10% ±5%
mains frequency 50/60Hz
connected load (max.) 250W 600W
overcurrent protection T 4A T 10A
cooling medium - R507 (CFC/HCFC free)
capacity (max.) 500 ml
speed – RPM 90 * 18000 rpm (step 1 rpm)
force – RCF 24270 x g (step 1 x g)
kinetic energy (max.) 8800 Nm
running time 00:00:01 ÷ 99:59:59 – [hours, min., sec] (step 1s)
time counting since start button is pressed / since preselected speed is reached
short-time operation mode – SHORT yes
continuous operation mode – HOLD yes
user programs 100
adjustable temperature - -20 ÷ 40*C* (step 1°C)
initial cooling (Fast Cool) no yes
guaranteed temperature with max.
rotor speed
cooling/heating without centrifuging no/no yes/no
cooling/heating with centrifuging no/no yes/no
acceleration (ACEL) 10 linear curves
deceleration (DECEL) 10 linear curves
programmable non-linear curves:
acceleration 10
deceleration 10
USB communication yes
Electromagnetic compatibility according to EN 61326-2-6:2006
ambient conditions PN-EN 61010-1 p.1.4.1
set-up site indoors only
ambient temperature 2° ÷ 40*C
humidity (maximum relative humidity) < 80%
excess-voltage category II EN 61010-1
pollution degree 2 EN 61010-1
safety area 300 mm
- ≤4*C
50Hz 60Hz
60Hz
Centrifuge user manual | 1
Degree of protection:
(according to PN-IEC 34-5)
dimensions: height (H) 320 mm
width (W) 365 mm
depth (D) 495 mm 660 mm
with open cover (Hoc) 665 mm
noise level 56 dB
weight 230V 28 kg 47 kg
weight 120V 29,5 kg 50,7 kg
* time and possibility of obtaining a set temperature is dependent on multiple factors, including: rotor type, established
RPM, ambient temperature; accuracy: - ±1*C appropriate for place of temperature sensor.
Menu languages: POLISH, ENGLISH, GERMAN, SPANISH, ITALIAN, PORTUGUESE, RUSSIAN, SWEDISH, FRENCH
(without national characters).
IP 21 IP 20
3 Installation
Open the package. Remove the box containing the accessories. Take out centrifuge from the container. Keep the box
and packing materials in case of service shipping.
3.1 Content of the package
name qty (pcs.)
centrifuge C0336 1
complete clamp 1
spanner for the rotor 1
emergency opening of the cover
power cord 230V / 120V 1
Fuse WTA T 4A 250V / WTA T10A 250V 2
petroleum jelly 20ml 1
USB A-A cable 1
user manual 1
3.2 Location
• The device is heavy, so lifting and carrying the centrifuge can lead to back injuries. Risk of injury
• Lifting and transporting of the centrifuge should be done with a sufficient number of helpers. Use
• The device should be lifted by the underside in the vicinity of the its feet and placed directly on
while lifting and carrying heavy loads.
a transport aid for transporting the centrifuge.
a suitable lab table.
2 | Centrifuge user manual
• Ensure safe location.
• The centrifuge shall not be located near source of heat and shall not be subjected to direct sunlight.
• Centrifuge should be flat-leveled. Effect of leveling shall be ensured by stable and flat-leveled table
top for the centrifuge.
• Centrifuge should be set horizontally on a rigid base.
• It is necessary to ensure a ventilation zone of the minimum 30cm round the centrifuge from every
direction. Do not veil ventilation holes!
• Table for centrifuge should possess safety zone of the minimum 30cm round the centrifuge from
every direction (safety needs in case of malfunction according to EN 61010-020.
• Table for centrifuge should be free of contaminants before locating of centrifuge.
• Passed parameters of the centrifuge are referring to the above named temperatures (see 2. Technical
specification).
• At the change of the place from cold to warm one, condensation of water will occur inside the centrifuge. It is important then that sufficient time be provided for drying the centrifuge prior to starting
the centrifuge again (min. 4 hours).
• Do not position the centrifuge so that it is difficult to operate the power switch
• Supply voltage given on the rating plate has to be consistent with local supply voltage. LABNET
INTERNATIONAL laboratory centrifuges are 1st safety class devices and they are provided with the
three-core cable with the plug resistant to dynamic loadings. Mains socket shall be provided with
the safety pin.
• It is recommended to install emergency cut-out that shall be located far from the centrifuge, near the
exit or beyond the room.
•
Before switching on, check whether the centrifuge is connected to power supply correctly. It is
obligatory to use only power cord recommended by manufacturer.
• Before using check whether the device is correctly installed.
3.3 Current protection
The centrifuge is equipped with thermal current protection. Fuse is situated in the plug-in socket unit
at back wall of the centrifuge.
4 Safety notes
4.1 Operating personnel
• Laboratory centrifuge can be operated by laboratory personnel after getting acquainted with user
manual.
• User manual shall be always held near the centrifuge.
• The centrifuge cannot be misused.
• If the centrifuge is used in a manner not specified by the manufacturer, the protection provided by
the device may be impaired.
4.2 Warranty
• Warranty period amounts to minimum 24 months (unless otherwise specified in the purchase documents).
• The service life of the centrifuge specified by the manufacturer amounts to 10 years.
• Manufacturer reserves the right to make technical changes in manufactured products.
• Maximum period of storage of not used centrifuge amounts to 1 year. After this period, a service
authorized by manufacturer should carry out technical inspection of the centrifuge.
Centrifuge user manual | 3
4.3 Loading the rotor
• Laboratory centrifuge can be operated by laboratory personnel after getting acquainted with user
manual.
• User manual shall be always held near the centrifuge.
• The centrifuge cannot be misused.
• It is necessary to insert test tubes symmetrically on the opposite sides.
FILLING TUBES
• Fill test tubes outside the centrifuge.
• Please pay special attention to the quality and proper thickness of the glass test tubes walls. Those
shall be test tubes for centrifuges.
• Fill test tubes outside the centrifuge.
4.4 Safety hints
ROTORS MAINTENANCE
• Lubricate the swing-out rotor journal pins.
• Use only accessories in good condition.
• Protect equipment against corrosion using accurate preventive maintenance.
HS ACCESSORIES MAINTENANCE
• HS accessories maintenance.
• Make sure that rubber O-rings are lightly coated with silicone grease.
HAZARDOUS MATERIALS
• Accessories are not biotight. For centrifuging infectious materials, it is necessary to use hermetically
closed tubes meeting demands of biotightness, in order to prevent germs migration into the centrifuge
and beyond it.
• It is not allowed to subject to centrifugation toxic materials with damaged leak proof seals of the
rotor or test-tube. Proper disinfection procedures have to be carried out when dangerous substances
contaminated the centrifuge or its accessories.
CORRECT
CORRECT
WRONG
WRONG
4 | Centrifuge user manual
EXPLOSIVE AND COMBUSTIBLE MATERIALS
• It is not allowed to centrifuge explosive and inflammable materials.
• It is not allowed to centrifuge substances prone to reacting in result of supplying high energy during
centrifugation. The centrifuge cannot be operated in explosion-endangered areas
• It is not allowed to centrifuge materials capable of generating inflammable or explosive mixtures
when subjected to air.
4.5 Maintenance conditions
START-UP
• Prior to switching the centrifuge on, one shall read carefully all sections of this instruction in order to
ensure smooth operation and avoid damages of this device or its accessories.
• In order to protect the centrifuge against unbalance, fill in the test tubes up to the same weight.
TRANSPORTATION
• Centrifuge must not be transported with the rotor mounted on the shaft.
GENERAL HINTS
• One must use original rotors, test-tubes and spare parts only.
• In case of faulty operation of the centrifuge one shall ask for assistance of service of LABNET
INTERNATIONAL company or its authorized representatives.
• It is not allowed to switch the centrifuge on if it is not installed properly or rotor is not fitted correctly.
CENTRIFUGES SUBSTANCES
• It isn’t allowed to exceed load limit set by the manufacturer. Rotors are intended for fluids of average
homogeneous density equal to 1,2 g/cm3 or smaller when centrifugation is carried out at maximum
speed. When fluids of higher density shall be used, then it is necessary to change density of centrifuges
sample in PARAM/DENSITY field.
4.6 Safety precautions
For safety reasons, inspections of the centrifuge carried out by the authorized service at least once a year after the
period of warranty. The reason for more frequent inspections could be corrosion inducing environment. Examinations should end with issuing report of validation that checks on the technical state of the laboratory centrifuge. It
is being recommended to establish document where every repairs and reviews are being registered. Both these
documents should be stored in the place of use of the centrifuge.
INSPECTION PROCEDURES CARRIED OUT BY THE OPERATOR
Operator has to pay special attention to the fact that the centrifuge parts of key importance due to
safety reasons are not damaged. This remark is specifically important as for:
• Centrifuge accessories and especially structural changes, corrosion, preliminary cracks, abrasion
of metal parts.
• Screw joints.
• Inspection of the rotor assembly.
• Inspection of bioseals of the buckets if such are used.
• Control of execution of the guarantee yearly technical inspection of the centrifuge
Only the manufacturer-specified holders, included in the equipment list, as well as centrifuge capillaries, which diameter, length and durability are suitable, should be used for spinning in this centrifuge.
The use of equipment made by other manufacturers should be consulted with the manufacturer of
the centrifuge.
• It is not allowed to lift or shift the centrifuge during operation, and rest on it.
• It is not allowed to stay in the safety zone within 30 cm distance around the centrifuge neither leave
within this zone some things, e.g. glass vessels.
• It is not allowed to put any objects on the centrifuge.
COVER OPENING
• It isn’t allowed to open the cover manually in emergency procedure when rotor is still turning.
Centrifuge user manual | 5
ROTORS
Fig.3. Assembly of angle rotor
4. Complete clamp
• It is not allowed to use the rotors and round carriers with signs of corrosion or other mechanical
defects.
• It is not allowed to centrifuge highly corrosive substances which may cause material impairment and
lower mechanical properties of rotor and round carriers.
• It isn’t allowed to use rotors and accessories not admitted by the manufacturer. Let to use commercial
glass and plastic test tubes, which are destined to centrifuging in this laboratory centrifuge. One
should absolutely not use poor quality elements. Cracking of glass vessels and test tubes could
result in dangerous vibration of the centrifuge.
• It is not allowed to carry out centrifugation with the rotor caps taken off or not driven tight.
4.7 Residual risk
The centrifuge is built according to the state-of-the-art and the recognized safety regulations.
Nevertheless, still remain some level of residual risk due to improper operation and malfunctions. It is possible to
decrease residual risk by strictly applying user manual conditions and correcting malfunction which could threaten
safety, immediately.
5 Operating
5.1 Centrifuge description
New generation of LABNET INTERNATIONAL laboratory centrifuges is provided with state-of-the-art microprocessor control systems, very durable and quiet asynchronous brushless motors and accessories consistent with
requirements of the present-day user.
5.2 Centrifuge overview
Fig.2. Right side of centrifuge
1. Motor axle
2. Rotor
3. Rotor lid
Fig.1. General view
6
5
4
3
2
1
4
3
2
1
Fig.4. Mains socket back
of the centrifuge
1
1. Main socket
2. Fuse socket
1. Power switch
2. USB
3. Control panel
4. Point of emergency lid opening
5. Lid
6. Inspection glass
2
5.3 Construction
6 | Centrifuge user manual
The centrifuge has rigid self-supporting structure. Housing was made of sheet aluminum, back made of steel
sheet. Front and cover was made of ABS type plastic. Cover is fixed on steel axles of hinges and from the front it
is locked with two electromagnetic locks blocking possible opening during centrifugation. Rotation chamber casing
was made of thick steel sheet. The rotation chamber bowl is made of stainless steel sheet. Rotors and containers
are from aluminum, lids from polycarbonate and reductive inserts from the polypropylene.
5.4 Rotor and accessories installation
• Connect the centrifuge to the mains (master switch on the back side of the centrifuge).
• Turn on the centrifuge (button on the side of the centrifuge).
• Open the cover of the centrifuge by pressing the COVER key (see section Centrifuging/Control Panel). Prior
to putting the rotor in, one has to check if the rotating chamber is free of impurities, e.g., such as dust, glass
splinters, residues of fluids that must be taken away.
• One shall fit the rotor on the motor shaft driving it home on the cone.
• Screw-in the bolt for fixing the rotor (clockwise) and screw it tightly home with the supplied spanner for the rotor.
• Swing-out rotors have to be provided with the buckets in all seats. One should remember that every buckets
swings individually. Bucket suspension studs should be lubricated periodically with petroleum jelly.
• In case of rotors designed with the cover they must not be used without it. Rotor covers must be closed exactly.
Rotor covers ensure smaller drags of the rotors, proper setting of the test-tubes and airtight sealing.
• One should use only buckets intended for selected types of the rotor.
• Fill test tubes outside the centrifuge.
• In case of centrifuging in an angle rotor, test tubes (buckets) have to be filled properly in order to prevent from
pouring fluids during centrifuging.
liquid
Tubes must be filled so that the material does not escape from
the reservoir during centrifugation.
centrifugal force
One shall fill tubes according to formula:
Max liquid level < Tube height – Internal tube diameter/2
Internal tube diameter
Tube height
Max liquid level
Observe the manufacturer’s restrictions about the filling of the test tube.
It is recommended to equalize vessels loads as much as possible in order to ensure minimal vibrations
during operation.
• In order to prolong lifetime of the rotor and gaskets rotors shall be lubricated with the maintenance oil, while
gaskets and threaded parts shall be lubricated with the petroleum jelly.
• For replacement of the rotor one shall unscrew clamping and then grab the rotor with both hands at opposite
sides, taking it away from drive shaft by pulling it up.
5.5 Control device
The microprocessor control unit of the centrifuge ensures broad possibilities of providing, realization and reading
of work parameters.
5.6 Setting parameters
Data setting and read-out system forms hermetically closed keyboard with distinctly accessible operation points.
Easily readable displays signaling individual performed operations facilitate operator’s programming and recording
of parameters and condition of the centrifuge.
The centrifuge is provided with the USB interface that enables connection of the centrifuge to external PC unit
with the printer and recording the centrifugation parameters.
Centrifuge user manual | 7
5.7 Safety features
Cover lock
The centrifuge can be started only with properly closed cover. While, the cover can be opened only after stopping the rotor. In case of emergency opening of the cover during operation, the centrifuge will be immediately
switched-off and the rotor will brake till complete stopping.
Unbalance detecting
When loads of opposite buckets or carriers in rotors are unbalanced, the drive will be switched-off during acceleration or operation of the centrifuge – and the error message will be displayed.
Rotor verification and checking compatibility with loaded program
Directly after starting centrifuging, a unit verifies the type of the rotor applied and in the case of its incompatibility
with the type indicated in the application or absence of the rotor, the spinning process shall be stopped with
simultaneous displaying the error message. The conformity of the type of the rotor is signaled with a single audible
signal. In case auto identification (see 9.8 Other) option is checked, proper rotor will be automatically chosen,
without user engagement.
Rest state inspection
Opening of the centrifuge’s cover is possible only with the rotor in the state of rest. When the rotor is being
stopped, the STOP diode is on and goes off when it is stopped. (Excepting emergency cover opening) – see p.
TROUBLESHOOTING.
Checking of excessive temperature
If temperature in rotation chamber exceeds 50°C (C0336) / 65°C (C0336R) caused by, for example, malfunction
of cooling system, drive will be switched off and error message will be displayed. The reboot is only possible after
chilling device.
5.8 Increase in temperature (C0336 only)
In uncooled centrifuges, the temperature in the rotor chamber, rotor and sample can increase to above 40°C,
based on the run time, g-force (RCF)/speed and ambient temperature.
6 Centrifuging
Power switching ON/OFF is carried out with master switch situated on the side wall of the centrifuge. All settings
on the centrifuge are done by means of the control panel.
6.1 Control panel
The control panel placed on the front casing serves the purpose of controlling centrifuge operation.
8 | Centrifuge user manual
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