Labnet BioPette E BE20-1, BioPette E BE200-1, BioPette E BE1000-1, BioPette E BE20-8, BioPette E BE200-8 Operation Manual
BioPette E
Single and 8-channel Electronic Pipettes
Operation Manual
Version: 2.7
www.labnetlink.com
Labnet InternationalLabnet International
PO Box 841PO Box 841
Woodbridge, NJ 07095Woodbridge, NJ 07095
USAUSA
Declaration of Conformity Declaration of Conformity
Product Name: BioPette E Single/8-channel Electronic Pipettes Product Name: BioPette E Single/8-channel Electronic Pipettes
Model Names: BE20-1, BE200-1, BE1000-1, BE20-8, BE 200-8 Model Names: BE20-1, BE200-1, BE1000-1, BE20-8, BE 200-8
All models comply with the following European standards: All models comply with the following European standards:
EMC: EN 55011, Class A EMC: EN 55011, Class A
EN 60601-1-2 EN 60601-1-2
To the best of my knowledge and belief, these units conform to these
standards.
To the best of my knowledge and belief, these units conform to these
standards.
Name: Mike Revesz Name: Mike Revesz
Position: Director of Quality Control Position: Director of Quality Control
Issue Date: 2003/4/30 Issue Date: 2003/4/30
Copyright Notice: Copyright Notice:
N
o part of this manual may be reproduced or transmitted in any
form or by any means, electronic or mechanical, for any purpose,
without prior written permission of Labnet International, Inc.
N
o part of this manual may be reproduced or transmitted in any
form or by any means, electronic or mechanical, for any purpose,
without prior written permission of Labnet International, Inc.
Table of Contents
1. Safety Precautions...................................................................................1
1.1. Operation Safety Precautions ..........................................................1
1.2. Battery Safety..................................................................................1
2. General Description................................................................................3
2.1. Features............................................................................................3
2.2. Ranges and Specifications...............................................................4
3. Getting S t arted ........................................................................................4
3.1. Unpacking........................................................................................4
3.2. Inserting the Battery ........................................................................5
3.3. Charging the Battery........................................................................6
3.4. Start Pipetting..................................................................................8
3.5. Hanger Installation ..........................................................................9
4. BioPette E Overview...............................................................................9
4.1. Keypad Function............................................................................13
4.2. Audible Signals .............................................................................13
5. Function Map ........................................................................................15
6. SYS (System) Mode...............................................................................16
6.1. Aspirating Speed............................................................................16
6.2. Dispensing Speed ..........................................................................17
6.3. Audible Signals .............................................................................17
6.4. Tip Ejector Alarm..........................................................................17
6.5. Blow-out........................................................................................18
6.6. Reverse Pipetting...........................................................................18
6.7. Setting PROG (Programs) .............................................................19
6. AUTO Mode 20
7. MD (Multiple Dispensing) Mode ......................................................... 20
8. MIX Mode 21
9. SE (Sequential Aspirating/Dispensing) Mode.....................................22
10.1. SA (Sequential Aspirating) Mode – Not available on BE1000-1
because the diameter of 1000ul tips does not allow the required air gap to be
maintained.
..................................................................................................22
10.2. SD (Sequential Dispensing ) M ode................................................24
10. Sterilization 26
11.1. Removal of the Lower Piston Assembly .......................................27
11.2. Reattach the lower piston assembly...............................................28
11. Maintenance 29
12. Troubleshooting.....................................................................................30
Appendix A: T echnical Specifications...........................................................32
Appendix B : Tip Ejector Height Adjustment..............................................32
Appendix C: Warranty...................................................................................33
1. Safety Precautions
Before using the BioPette E for the first time, please read this entire
operating manual carefully. To guarantee problem free, safe operation of the
BioPette E, it is essential to observe the following points:
1.1. Operation Safety Precautions
When using infectious, radioactive, toxic and other solutions which
may pose health risks, please observe appropriate safety precautions.
1. Do not use the BioPette E in a potentially explosive environment or
with potentially explosive chemicals.
2. When using organic solvents or corrosive chemicals, please check their
compatibility with the pipette tips and the BioPette E.
3. Repair should be carried out by Labnet authorized service personnel
only. Contact Labnet Internatio nal, Inc. for information on authorized
service options.
4. Use original spare parts and accessories only.
1.2. Battery Safety
The BioPette E operates on a small but powerful Lithium-ion battery.
Misuse or abuse of the Lithium-ion battery may cause damage or injury
through fire, electric shock, or chemical leakage. Please read and
understand all warnings before using the battery.
1. When storing the battery, do not allow it to come into contact with any
metallic surfaces.
2. Never use the BioPette E while using the DC-in jack to charge the
battery.
1
2
3. Do not incinerate the Lithium battery or expose it to excessive heat.
4. Do not short-circuit, puncture, crush, disassemble, damage, force
over-discharge (reversal) or modify the battery.
5. Do not expose the battery to water or moisture.
6. Do not drop or subject the battery to strong impacts.
7. Only use the battery specified in this BioPette E manual.
8. Only use the specified, battery charging unit or DC-in jack to charge
the battery.
9. Do not use a leaking battery.
10. If charging is not completed within the specified time period, unplug
the charger and discontinue charging immediately.
11. The charger and battery temperature rises with extended periods of use.
Care should be taken to avoid burns.
12. Bu rns may result if the battery is removed immediately after extended
periods of use.
13. If fluid from the battery enters your eye, immediately rinse the eye with
plenty of fresh water and contact a doctor. If fluid from the battery
makes contact with your skin or clothing, wash the area thoroughly
with water.
2. General Description
Labnet's BioPette E is designed to replace the traditional manual pipette. It
features an accurate and efficient, as well as ergonomic and light-weight
design. Researchers can program this state-of-the-art product to conduct
different pipetting tasks. More accurate and comfortable pipetting can be
easily achieved without any concern of finger stress or injury.
2.1. Features
z High accuracy and precision
z Low forces
z Useful, practical operation protocols, such as Automatic
Pipetting(AUTO), Multiple Dispensing(MD), Mixing(MIX), and
Sequential Aspirating/Dispensing (SE), etc.
z 9 user-defined program sets
z 5 speeds for aspirating and dispensing
z Li-ion battery provides extended use on a single battery charge
z Ergonomic design
z Automatic calibration
z User-friendly graphic operation interface.
z Adjustable tip ejector.
z Automatically remembers the last-used pipetting protocol and settings
z Complies with CE, ISO-8655, GLP
3
2.2. Ranges and Specifications
Product No. Channels
Volume
Range (µl)
Increment
(µl)
Accuracy
(Rel.±)
Precision
( Rel. CV
)
BE20-1-X 1 2 -20 0.1
7.0-1.0% 2.0-0.3%
BE200-1-X 1 10 -200 1 2.5-0.8% 1.0-0.15%
BE1000-1-X 1 100 -1,000 1 3.0-0.8% 0.6-0.15%
BE20-8-X 8 2-20 0.1 7.0-1.0% 2.0-0.3%
BE200-8-X 8 10-200 1 3.5-0.8% 1.0-0.15%
Note:
1. X: power plug, no designation=U.S. plug, EU=European plug,
UK=U.K. plug, AU=Australian plug
2. BioPette E Accuracy and Precision Specifications have been attained
using Labnet brand pipette tips. Labnet guarantees Accuracy and
Precision only if Labnet brand tips or other approved tips are used.
3. Getting Started
3.1. Unpacking
Open the BioPette E package and confirm that all items are included:
z Single/8-channel BioPette E unit z Operation manual
z Quality assurance card z AC-DC power adaptor
z BioPette E hanger and
accessories
z Pipette tips
4
If there are any items missing, damaged, or not according to your order,
please contact your distributor or sales representatives for replacement
immediately.
Please charge the battery before first-time operation.
3.2. Inserting the Battery
Figure 1: Open the battery cover. Figure 2: Insert the battery.
Remove the battery cover by pressing the latch (see Figure 1). Insert the
battery (see Figure 2). Metallic contacts must be face down. Close the cover
of the battery compartment. Press the
RESET
button to power on and
calibrate the BioPette E.
Note: After a charged battery is inserted, the BioPette E will automatically
carry out a calibration routine.
5
3.3. Charging the Bat tery
BioPette E’s Internal Charging Circuit
Optional Charger
There are two ways to charge the battery: (1) Use BioPette E’s internal
charging circuit, (2) Use the optional charger.
Insert the battery into the battery compartment. Insert the DC-in plug of the
power adaptor into the DC-in jack (see Figure 3). Connect the power plug
of the power adaptor with the external power source. Connecting the power
plug before inserting the battery will inactivate the charging process. The
indication bar inside battery symbol
will blink during the charging
process. When the battery is fully charged, the blinking will stop and the
BioPette E will beep once to remind the user.
Connect the DC-in plug of the AC-DC power adaptor to the charger.
Connect the power plug of the AC-DC power adaptor to the external power
source. The indication light will turn green. Insert the battery (see Figure 4).
Once the battery is correctly inserted and charging, the indicator light will
turn red. When the battery capacity is full, the indicator light will turn green
again.
6
Figure 3: Using BioPette E’s internal
charging circuit to charge the battery
Figure 4: Using the optional
charger to charge the battery
Note:
1. For safety reasons, the BioPette E cannot be used during charging.
2. Please do not over-charge the battery for a long period of time.
7
3.4. Start Pipetting
Figure 5: Correct hand position
There is no power On/Off button on the BioPette E. Once users insert the
fully-charged battery, the BioPette E will be switched on. It will perform a
calibration routine and switch on the LCD display as in Figure 8. Press any
keypad button or the
PLUNGER button to enter the function mode selection.
The BioPette E will be automatically powered off if it’s not in use for more
than 10 minutes.
Select the desired function mode and settings (refer to the following
chapters : 6 ~ 10 ) before pipetting. After the initial set-up, attach the
correct-sized tips to the cone(s) of BioPette E before operation.
The BioPette E is an ergonomic instrument designed to reduce hand fatigue
and repetitive stress injury. In orde r to maximize the benefits of the
BioPette E’s design, the instrument should be held as shown in figure 5.
Use your index finger to press the
PLUNGER button and use your thumb to
press the
TIP
ejector.
8
3.5. Hanger Instal lation
The BioPette E hanger can be attached to a shelf or counter using the screw
or sticker as shown in Figure 6. The finger rest of the BioPette E locks into
the hanger and keeps the BioPette E in a vertical position.
Figure 6: BioPette E hanger installation
4. BioPette E Overview
This section presents an overview of the BioPette E’s various components
and buttons (see Figure 7) as well as the symbols and indicators on the
LCD display (see Figure 8).
9
LCD Display
10
Figure 7: 1-channel BioPette E overvi ew
Keypad
Tip Ejector
Bushing
Lower Part
Cone
Battery
C
"RESET
"
"PLUNG
Finger R
Connect
i
(Front View) (Side View)
Figure 1 : Overview
11
12
Figure 8: LCD display Panel
4.1. Keypad Function
Button Symbol Function Description
Set/Enter
Confirms your choice
Exit/Esc
Exits to previous mode/setting
Up
Increases volume/ changes parameter
Down
Decreases volume/ changes parameter
Reset
RESET
Piston automatically determines the
home position
PLUNGER
PLUNGER
Activates aspirating/dispensing/
same as
button
Tip Ejector
TIP
Ejects the tips
Tips for Efficient Operation: The
PLUNGER button can be used to increase
volumes and to change modes and settings same as the “Up” button during
the function mode selection and volume settings. This operatio n feature can
speed up the setting processes.
4.2. Audible Signals
The audible signals are beneficial when you are familiarizing yourself with
the operating procedure of the BioPette E. They can also be switched off if
desired (see Section 6.3.)
13
14
Audible Sound Operation
Low-tone beep Aspirating
High-tone beep Dispensing/ Tip Ejection/ Error codes
2 beeps
MD/MIX/SE mode cycle or RESET routine is
completed
5. Function Map
The main menu of the BioPette E includes five function modes (SYS,
AUTO, MD, MIX and SE). Each mode includes various function settings
(see Figure 9).
SYS
AUTO
MD MIX SE
Function
Mode
Aspirating
Speed
Dispensing
Number
(COUNT)
Basic
Aspirating/
Dispensing
Mix
Number
(
COUNT
)
Dispensing
Speed
Audible
Signals
Program
Recall
Reverse
Pipetting
Tip Ejection
Alarm
Blow-out
Dispensing
Vol ume
Mix
Vol ume
SD SA
Aspirating
Number
(1,2,3,…)
Dispensing
Number
(1,2,3,…)
Aspirating
Vol ume
Dispensing
Vol ume
Function
Settings
Function Map
Figure 9: Function map
15
6. SYS (System) Mode
Note :
1. These 7 function settings are sequential procedures. To store these
settings in memory, users have to complete the whole setting procedure
and press
«SET» when the LCD displays .
SYS mode is used to set up the function settings before pipetting. Once
users select and store these settings, they will no longer need to set up the
settings before every pipetting. There are 7 function settings (Aspirating
Speed, Dispensing Speed, Audible Signals, Tip Ejection Alarm, Blow-out,
Reverse Pipetting and Program Recall) in SYS mode.
2. Users can use the
«Up/Down» button to move back and forth to the
previous function settings.
6.1. Aspirating Speed
1. Press
«EXIT» to enter the function mode selection. Then press
«Up/Down» to select the
symbol (flashing).
2. Press
«SET» to confirm the selection of
mode and the
aspirating speed symbol
will start flashing.
There are 5 speeds available for both aspirating and dispensing:
3. Press
«Up/Down» to change the aspirating speed ( : Slowest, :
Fastest).
4. Press
«SET» to confirm the speed selection and move to the
“Dispensing Speed” function setting.
16
6.2. Dispensing Speed
1. The “Dispensing Speed” symbol
should now be flashing.
2. Press
«Up/Down» to change the aspirating speed ( : Slowest, :
Fastest).
3. Press
«SET» to confirm the speed selection and move to the
“Audible Signals” function setting.
6.3. Audible Signals
1. The “Audible Signals” symbol
should now be flashing.
2. Press
«Up/Down» to select the preference ( : On, : Off).
Please refer to Section 4.2 for the definition of “Au dibl e Si gnal s”.
3. Press
«SET» to confirm the selection and move to the “Tip
Ejection Alarm” setting.
6.4. Tip Ejector Alarm
1. The “Tip Ejector Alarm” symbol
should now be flashing.
2. Press
«Up/Down» to select the preference ( : On, :
Off).
The tip ejector alarm is designed to remind the users that the tips have been
ejected. It helps users to avoid using the same tip to contaminate different
reagents.
3. Press
«SET» to confirm the selection and move to the “Blow-out”
setting.
17
6.5. Blow-out
1. The “Blow-out” symbol
should now be flashing.
2. Press
«Up/Down» to select the preference ( : On, :
Off).
The automatic blow-out function, in different pipetting modes, simulates
the blow-out function in manual pipettes. To avoid the leftover final droplet
in tips, the blow-out setting is recommended in all protocols. The blow-out
setting will not be performed in “Reverse Pipetting” setting.
3. If
is selected, pressing «SET» will confirm the selection and
move to the “PROG” setting. If
is selected, pressing «SET»
will confirm the selection and move to the “Reverse Pipetting” setting.
Note: It is important to withdraw the tip quickly from the dispensing sample
vessel after dispense if Blow-out is selected, because the piston will retract
a short distance for creating the blow-out volume.
6.6. Reverse Pipetting
1. The “Reverse Pipetting” symbol
should now be flashing.
2. Press
«Up/Down» to choose the preference ( : On, :
Off).
The automatic reverse pipetting, in different pipetting modes, is to reserve
the final drop of sample. The BioPette E will not perform the reversed
pipetting in MIX or SE modes.
3. Press
«SET» to confirm the selection and move to the “PROG”
setting.
18
Note: If Reverse Pipetting is selected, users have to press the
PLUNGER
button one more time after pipetting to dispense the remaining liquid
during the pipetting.
will appear on the LCD to remind users to
dispense the remaining liquid.
6.7. Setting PROG (Programs)
Store Operating Mode to Storage Locations
The memory program includes 9 different storage locations (PROG 01 ~
09). Your favorite operating modes with user-selected settings can be stored
to these locations for future recalls. Before setting the programs, you must
have programmed operating mode (e.g. AUTO, MD mode, etc).
1. Complete the setup of function mode (e.g. AUTO, MD, MIX mode).
2. Press and hold
«SET» for 3 seconds to enter PROG mode.
3. Press
«Up/Down» to select PROG number from 01 ~ 09.
4. Press
«SET » to confirm the location selection and save the
functions.
Recall Stored Programs from the Storage Location
1. Press
«EXIT» to enter the function mode selection. Then press
«Up/Down» until the
symbol starts flashing.
19
2. Press
«SET» repeatedly to confirm the selection of all 6 function
settings until the
symbol starts flashing.
3. Press
«Up/Down» to select the PROG number.
4. Press
«SET» to confirm the selection.
5. Press the
PLUNGER button to operate the BioPette E in the selected
program.
Note: During the recall process, the changes of other settings will not b e
stored.
6. AUTO Mode
1. Press «EXIT» to enter the mode selection, which causes the
symbol to start flashing. If not, press
«Up/Down» to select AUTO
mode.
In AUTO mode, the BioPette E performs the aspirating and dispensing of a
set liquid volume.
2. Press
«SET» to confirm the mode selection.
3. Use
«Up/Down» to select the desired pipetting volume.
4. Press
«SET» to confirm selected volume and the BioPette E is
ready for pipetting.
Tip: Users can use the
PLUNGER button instead of «Up» to speed up
the volume setting.
7. MD (Multiple Dispensing) Mode
1. Press «EXIT» to enter the mode selection.
20
2. Press
«Up/Down» to make the symbol flash.
In MD mode, the BioPette E performs repetitive dispensing of a selected
volume. For best accuracy using the Multidispensing Mode it is
recommended to select Reverse Pipetting in System Mode and blow out the
residual volume in the end of every multiple dispensing cycle. If the
reversed function is performed, the sum of the dispensing aliquots and an
automatically selected excess volume is aspirated into the tip. The excess
volume ensures the best accuracy during multidispensing.
3. Press
«SET» to confirm the selection. The symbol will
then appear.
4. Use
«Up/Down» to select the desired pipetting count.
5. Press
«SET» to confirm the selection of count. The default
pipetting volume will start flashing.
6. Use
«Up/Down» to select the desired pipetting volume.
7. Press
«SET» to confirm the pipetting volume. The pipetting
volume will show the total aspirating volume.
8. After the settings are completed, first press the
PLUNGER button to
aspirate the sample according to the pipetting volume. Then press the
PLUNGER button repeatedly to dispense the samples according to the
pipetting counts. For the last count, you will hear two beeps to indicate
that the next dispense is the last. If using the recommended Reverse
Pipetting mode, the display will show
to remind the user to
press the
PLUNGER button and dispense the extra remaining amount of
liquid. After completing the Multiple Dispensing cycle, pressing the
PLUNGER button again will aspirate liquid into the tip and repeat the set
MD cycle.
8. MIX Mode
21
1. Press «EXIT» to enter mode selection.
2. Press
«Up/Down» repeatedly until the symbol starts
flashing.
After the settings are completed (see instructions below), Mixing is
performed automatically by pressing the
PLUNGER button once.
3. Press
«SET» to confirm the selection. The symbol will
then appear.
4. Use
«Up/Down» to select the desired mixing count.
5. Press
«SET» to confirm the selection. Th e default pip etting volume
will start flashing.
6. Use
«Up/Down» to select the desired mixing volume.
7. Press
«SET» to confirm the selection. Th e total mixing volume and
counts will be displayed.
8. Press the
PLUNGER button to start the mixing function. After the
completion of mixing, pressing the PLUNGER b utton will re-activate th e
mixing process until the user changes the functio n mod e .
9. SE (Sequential Aspirating/Dispensing) Mode
SE mode includes SA (Sequential Aspirating) mode and SD (Sequential
Dispensing) mode.
10.1. SA (Sequential Aspirating) Mode – Not available on BE1000-1
because the diameter of 1000ul tips does not allow the required air
gap to be maintained.
1. Press
«EXIT» to enter function mode selection.
22
2. Press
«Up/Down» repeatedly until the
symbol starts flashing.
The BioPette E performs repetitive aspirations of the selected volumes. An
air gap will be created automatically between two aspirations. When the
sequential aspiration is completed, the next operation will dispense all of
the aspiration volume together.
3. Press
«SET» to confirm the selection.
4. Use
«Up/Down» until the symbol starts flashing.
5. Press
«SET» to confirm the selection.
6. Under the
symbol, “01” will appear. Press «Up/Down» to
set the volume of the first aspiration.
7. Press
«SET» to confirm the selection.
8. Repeat steps 6 and 7 to set the sequential aspiration volumes. The total
aspirating volume (including air gap volume) cannot exceed maximum
volume range.
9. Press
«SET» to confirm the selection.
10. Th e count “01” and aspirating volume will appear on the display. The
pipetting direction and speed
will flash to indicate that the BioPette
E is ready for liquid pick-up. Press the
PLUNGER button to aspirate
the samples. will appear to remind users to create an air gap (by
pressing the
PLUNGER button with the tip out of the sample) between
sample aspirations.
11. When the aspirations are completed, the BioPette E will make two
beeping sounds and
will flash to indicate the BioPette E is ready
for dispensing.
12. After th e sequential aspirating cycle has been completed and the liquid
has been dispensed, pressing the
PLUNGER button will re-activate the
whole cycle again until users change the function mode.
Note:
1. If the blow-out setting is selected, the BioPette E will automatically
blow-out the liquid during sequential aspiration mode.
23
2. A1000 models do not include the Sequential Aspirating (SA) function,
because the air gap is not strong enough to support the weight of
samples.
3. Sequential Aspirating (SA) function will n ot perform Reverse Pipetting
even it is selected in System (SYS) mode.
10.2. SD (Sequential Dispensing) Mode
24
1. Press
«EXIT» to enter function mode selection.
2. Press
«Up/Down» repeatedly until
symbol starts flashing.
The BioPette E performs repetitive dispensing of various selected volumes.
As with Multiple Dispensing mode, for best accuracy in SD Mode, it is
recommended to select Reverse Pipetting in System Mode.
3. Press
«SET» to confirm the selection.
4. Press
«Up/Down» repeatedly until symbol starts flashing.
5. Press
«SET» to confirm the selection.
6. Under
symbol, “01” will appear. Press «Up/Down» to set
the volume of the first dispense.
7. Press
«SET» to confirm the selection.
8. Repeat steps 6 and 7 to set the sequential dispensing volu mes. The total
dispensing volume cannot exceed the maximum volume range.
9. Press
«SET» to confirm the selection.
10. The count “01” and the total aspirating volume will appear on the
display. The pipetting direction and speed
will flash to indicate the
BioPette E is ready for liquid pick-up. Press the
PLUNGER button to
aspirate the sample. When the aspiration is completed, the dispensing
count and volume will appear and
will flash to indicate that the
BioPette E is ready for dispensing.
11. Th e BioPette E will make two beeping sounds to remind the user of the
last measured volume to dispense. At the end of the sequential
dispensing cycle, if Reverse Pipette mode is selected,
will be
displayed to remind the user to press
PLUNGER bu tton to the dispense
the remaining extra amount of sample. After completing the
dispensing, pressing the
PLUNGER button again will re-activate the
whole cycle until the user changes the function mode.
Note: If the blow-out setting is selected, the BioPette E will automatica lly
blow-out the liquid at the end of a sequential dispensing cycle.
25
10. Sterilization
Only the lower piston assembly of BioPette E can be steam-autoclaved
(121
o
C, 1 bar, 20 m i nut es). NOTE: It is important not to exceed the the
temperature of 121C or 20 minute time during the autoclaving cycle.
Autoclaving at a higher temperature or for longer period of time will
damage the manifold and void the warranty. The autoclaved parts
must be allowed to dry completely at room temperature for at least 2 hours
before reassembly. Users can follow Figures 11~14 to disassemble BioPette
E. Please “DO NOT” use excessive force to pull do w n the lowe r part
before releasing the cylinder lock as shown in Figure 10. This action will
break the connection mechanism of the lower part, permenently damaging
the instrument and will void the warranty.
Don't use force to pull the lower portion before
releasing the cylinder lock.
Cylinder lock
Figure 10: Incorrect removal of the lower piston assembly.
26
11.1. Removal of the Lower Piston Assembly
Always remember to press
RESET
button before you disassemble the
lower part of BioPette E.
1. Press the
RESET
button. Wait until you hear two beeps.
2. Loosen the lower part of BioPette E by unscrewing the connecting nut
in counter-clockwise direction as shown in Figure 11.
3. Pull the lower piston assembly downward slowly until the metal
cylinder lock appears as shown in Figure 12.
4. Lay the BioPette E down on a flat surface. Push the metal cylinder
lock downward until you he ar a click sound as shown in Figure 13.
5. The lower piston assembly will come off easily and automatically as
shown in Figure 14.
Figure 11: Loosen the connecting nut
by unscrewing counter-clockwise.
Figure 12: Pull the lower
p
iston
assembly downward slowly until
the cylinder lock appears.
27
Cylinder lock
Figure 13: Push the metal cylinder lock
downward until you hear a click sound.
Figure 14: The lower piston
assembly will come off easily and
automatically.
11.2. Reattach the lower piston assembly
Always remember to press
RESET
button after you remove the lower
piston assembly of BioPette E.
Follow these steps to reassemble BioPette E:
1. Press the
PLUNGER bu tton or any button of the keypad to make th e step
motor shaft retract about 1cm back into the handle. The step motor will
automatically retract inside if users do not touch any button for 10
minutes.
2. Connect the lower part with connecting nut. Tighten up the connecting
nut.
28
3. Press the
RESET
button. The step motor shaft will connect with the
piston of the lower part automatically. After the connection is
completed, the BioPette E will beep twice.
11. Maintenance
The outside of BioPette E may be wiped clean wi t h 60 % Is op r opanol, 70%
ethanol or mild detergent, and then wiped dry with a lint-free cloth. If the
pipette is severely contaminated or if very corrosive chemicals are
dispensed, the lower part of the BioPette E should be disassembled. The
individual parts should be rinsed in distilled water and then air dried. For
complete information on proper disassembly of the BioPette E, contact
Labnet’s service department.
It is recommended to clean the BioPette E at regular intervals depending on
how much the pipette is used, as well as lubricating it once per year. If the
pipette is autoclaved often, you may need to lubricate the pipette more
frequently. Lubrication of the piston should be performed only by
authorized Labnet service personnel, or trained users, and only the proper
BioPette E lubricant available from Labnet International should be used*.
For complete information on proper service of your BioPette, contact
Labnet International’s Service Department.
It is recommended to check the performance of your BioPette E regularly
(e.g. every 3 ~ 6 months) and always after maintenance or service. More
frequent testing of the BioPette E may be desired depending upon
requirements of the application, freq uency o f use, number of operators
using the pipette, nature of the liquid dispensed and the acceptable
maximum permissible errors established by the user (ISO 8655-1). For
more information of BioPette E calibration procedures, users are welcome
to visit out web site at www.labnetlink.co m.
29
*Note: Using improper lubricants will deter or block the movement of
BioPette E’s piston.
12. Troubleshooting
To ensure the product’s quality and performance, the BioPette E is designed
with a self-diagnosis program. This program will constantly monitor the
accuracy of pipetting volume, battery stat us and auto-calibration function.
Error (Err) messages will appear on the display if the BioPette E fails to
perform the attempted action properly. In the case of Error (Err) messages
or faults, please refer to the solutions in the following table to clear the
error messages or faults.
If the following solutions are not able to resolve the problem, please contact
Labnet International’s Service Department to arrange for authorized service.
It is also recommended to check Labnet Internatio nal’s web site at
www.labnetlink.com for useful and current technical information.
30
Symptom Possible Cause Solution
Bad battery Charge the
battery
“Err 01”
Bad power adaptor Replace the
power adaptor
“Err 02” Auto-calibration is not
working
Press the
RESET
button
“Err 03” Inaccurate pipetting volume
Press the
RESET
button
Step motor failure “Err 04”
Photo-couple failure
Press the
RESET
button
Droplets left inside
the tip
Unsuitable tip Use quality, low
retention tips
Leakage or volume
too small
Non-uniform wetting of the
plastic tip
Rinse the tip once
prior to pipetting
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Tip incorrectly attached Attach firmly
Unsuitable tip Use high-quality
tips
Low battery Charge the
battery
The lower manifold is not
correctly attached
Detach and
reassemble
Foreign material blocking
the hole at bottom of the
cone
Use MIX mode
and distilled
water to wash. Air
dry
Failure to aspirate
Piston movement is blocked Lubricate piston
Bad battery contact Reinsert the
battery
Rusted battery contact Replace with new
battery
Power on failure
Dead battery Charge the
battery
Function Mode
setup failure
Tip Ejector can’t bounce
back
Adjust Tip
Ejector position
Appendix A: T echnical Specifications
Operation Mode
Automatic Pipetting (AUTO), Multiple
Dispensing (MD), Mixing (MIX),
Sequential Aspirating/Dispensing (SE),
System Setup (SYS)
Program memory
9 programs
Aspirating/Dispensing Speed
5 speeds
Auto-calibration
Yes
Piston Motor
High precision stepping motor
Power Saving Feature
Yes, after 10 minutes
Power Adaptor
100/120/220/240 V AC-DC 3.6 V
Audible Alerts
Yes
Autoclavable
Yes, lower manifold only
Operating Temperature
5 ~ 50
o
C
Operating Humidity
RH: 0 ~ 85%
Battery
900 mAh/3.6 V or above, Li-Ion
Battery Charger
Optional
Stand for 3 units
Optional
Certification
Complies with CE, Class A
(EN60101-1-2, EN50082-1, EN 55011)
Complies with ISO-8655/ DIN 12650
Note:
1. U.S. patents pending
2. Specifications are subject to change without prior notice
Appendix B : Tip Ejector Height Adjustment
1. Remove the lower manifold the of BioPette E as described in Section
11.1.
2. Remove the flat-head scr ew and bushing as shown in Figure 15.
3. Use flat-head screw driver or other tool to rotate the tip ejector height
adjustment nut. By rotating the nut in counter-clockwise dir ection, the
tip ejector can be adjusted down. This height adjustment feature enables
efficient ejecting of different manufacturer’s tips.
32
Tip ejector height
adjustment nut
Flat-head screw
Figure 15 Tip ejector height adjustme nt
Appendix C: Warranty
Labnet’s BioPette E electronic pipettes are covered by a warranty for one
year against defects in materials and workmanship. This period begins from
the date of purchase, and within this period all defective parts will be
replaced at no charge by Labnet International. The warranty does not cover
defects caused by excessive wear and tear or damage due to shipping,
accident, abuse, misuse, problems with electrical power, or usage not in
accordance with product instructions, or if other than original spare parts
supplied by the manufacturer have been used. Each BioPette E electronic
pipette is tested and documented by the manufacturer before shipping.
Labnet’s Quality Control System guarantees that the performance of the
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Labnet International Inc., PO Box 841, Woodbridge, NJ 08837
TEL: +732-417-0700 FAX: +732-417-1750
US Toll Free: 888-522-6381
E-mail: Labnet@labnetlink.com
Web Site:
www.labnetlink.com
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