Labnet Biopette A Autoclavable Multichannel Pipettes User Manual

MULTICHANNEL
Labnet International, Inc.
MULTICHANNEL
A
A1 A2
B
F
D
H
E
MULTICHANNEL
4A 4B 4C 4D
G
MULTICHANNEL
CONTENTS
1 - INTRODUCTION 2 - VOLUME SETTING 3 - METHOD OF PIPETTING 4 - RECOMMENDATIONS 5 - RECALIBRATION 6 - CLEANING AND STERYLIZATION 7 - PIPETTE KIT AND ACCESSORIES 8 - SPARE PARTS
1 - INTRODUCTION
The BioPette™ series of adjustable multichannel pipettes have been designed for the filling of la­boratory microplates. The pipettes enable precise and simultaneous delivery of 8 or 12 preset-vol­ume doses of liquid.
BioPette™
pipettes are produced in four ranges of volumes: 1-10 µl, 5-50 µl, 20-200 µl, 50-300 µl.
The BioPette™ pipettes are equipped with a di­gital
counter which shows the pipetting volume. The set volume is visible in the window on the handle. The setting of the volume is done by tur
­ning of the pipetting pushbutton knob (Fig. 1A2) or by turning of the black knurled adjustment knob (Fig. 1B) in the right direction. The volume range is shown on the pipetting pushbutton (Fig. 1A1).
BioPette™
pipettes should be used with poly­propylene disposable tips, to ensure safety and accuracy, (Fig. 1H). The shafts without O-rings ensure the compatibility of the pipettes with a broad assortment of tips, and the special ejector shape reduces the force required for their ejec­tion. In order to protect the user against inad­vertent contact with used tips, the pipettes are equipped with tip ejectors, (Fig. 1F). By turning the multichannel module clockwise in relation to the handle, (Fig. 2), it is possible to disable the ejector’s operation in order to avoid acci­dental use, or to change its position in such a way as to make filling of microplates more con­venient.
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The specifications for accuracy and precision given in the following table are obtained using
Labnet
tips. These figures are only guaranteed
Labnet
when
BioPette 8
Model
BPE-10 5 ±4.0 ± 2.0
BPE-50 25 ±3.0 ± 1.2
BPE-200 100 ±1.5 ± 0.8
BPE-300 150 ±1.2 ± 1.0
BioPette 12
Model
BPT-10 5 ±4.0 ± 2.0
BPT-50 25 ±3.0 ± 1.2
BPT-200 100 ±1.5 ± 0.8
BPT-300 150 ±1.2 ± 1.0
tips are used.
channel
SPECIFICATIONS
Volume Accuracy Precision
[µl] [%] [%]
Min 1 ±8.0 ± 6.0
Max 10 ±2.0 ± 1.2
Min 5 ±4.0 ± 2.5
Max 50 ±1.6 ± 0.6
Min 20 ±3.0 ± 1.5
Max 200 ±1.0 ± 0.6
Min 50 ±1.6 ± 1.5
Max 300 ±1.0 ± 0.6
channel
SPECIFICATIONS
Volume Accuracy Precision
[µl] [%] [%]
Min 1 ±8.0 ± 6.0
Max 10 ±2.0 ± 1.2
Min 5 ±4.0 ± 2.5
Max 50 ±1.6 ± 0.6
Min 20 ±3.0 ± 1.5
Max 200 ±1.0 ± 0.6
Min 50 ±1.6 ± 1.5
Max 300 ±1.0 ± 0.6
These specifications are obtained in forward mode, using a gravimetric method with the tem­perature of the distilled water, tips and all other conditions stabilized between 19°C and 21°C. The values given include all components of error due to both normal hand warming and changing of the tips.
Performance tests: The pipette is calibrated in accordance with EN ISO 8655. Performance can be verified by checking the pipette using
MULTICHANNEL
the procedures outlined in the EN ISO 8655 standard.
The pipette design enables the user to perform the recalibration process according to the rules presented in section 5.
If the knob is accidentally turned too far, ie is set lower than the required value, the process must be repeated. The approach to the set vol­ume must always be made in the order of decreasing value.
2 - SETTING THE VOLUME
Volume is set using the pipetting pushbutton (Fig. 1A2) or while the value is shown
black adjustment knob (Fig. 1B),
on the digital counter. The volume shown by the counter is represented by three digits which should be read from top to bottom. Typical readings are shown in the fol­lowing table:
Model
BPE-10
BPT-10
BPE-50
BPT-50
BPE-200
BPT-200
BPE-300
BPT-300
Counter
readings
0 3 5
0 6 5
0 8 5
2 5 0
Set
volume
3.5 µl
6.5 µl
85.0 µl
250.0 µl
Increments
0.02 µl
0.10 µl
0.20 µl
1.0 µl
To attain the maximum accuracy, set volume must be approached from a higher value. If the desired value is lower than the previous value, simply use the knob in the pushbutton (Fig. 1A2) or the black adjustment knob (Fig. 1B) to adjust the value down to 1/3 turn above the required set­ting. If the desired value is higher than the pre­vious value, use the knob in the pushbutton or the black adjustment ring to adjust the value to 1/3 turn above the required value. The setting should then be performed in the following manner:
With the volume set to 1/3 above the required setting, slowly turn the pipetting pushbutton knob or the black adjustment knob to the required volume.
3 - METHOD OF PIPETTING
Fit the tips onto cones of the multichannel module. In order to put tips on the multichannel shafts hold the pipette vertically and press it against the tips in the rack box, until the shafts about 1.5 mm into the multichannel module.
retreat
The suspension system of the shafts ensures even and tight sealing of tips. The rolling movement does not have to be performed to seal the tips tightly. The liquid aspirated into the tips should not flow out by gravity from properly fixed tips. While holding the pipette in a vertical position, tips should be immersed in the liquid to a depth of 2-4 mm, and flushed once by drawing a dose of liquid and dispensing it out with slow and steady movement.
Next, still holding the pipette vertically, the ope­rator should press the pipetting button until the first resistance point is felt, and immerse the tips in the liquid to the depth of 2-4 mm, (Fig. 3B). Releasing the pipetting button with a slow and uniform movement during 2-3 seconds, the liquid should be drawn into the tips, (Fig. 3B) which should then be lifted above the liquid’s surface. The pipette should next be positioned at an angle of 10-45 degrees in relation to inner walls of destination vessels and tips should be emptied by pressing the pipetting button slowly until the first resistance point is felt, (Fig. 3C). After waiting one second, the pipetting button should be pressed to the second resis­tance point in order to expel remaining liquid, (Fig. 3D). Then tips should be lifted out from the vessels while maintaining contact between the ends of the tips and the inner walls of the vessel until the pipetting button has been rela­sed, (Fig. 3E). Finally, pressing the ejector but­ton, the operator should separate the tips from multichannel module’s cones, (Fig. 3F).
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MULTICHANNEL
4 - RECOMMENDATIONS
To achieve maximum safety, precision and re­liability, the following principles should be ob­served:
• do not draw liquids without tips fitted on the pipette cones,
• do not lay down the pipette with tips filled,
• do not draw volumes of liquid exceeding the pipette’s range,
• check if the tips are fitted properly,
• during operation, the pipette must be held ver­tically, tips should be immersed in liquid to the depth of 2 to 4 milimeters and the pipet­ting button should be depressed and released slowly and evenly,
• new tips must be pre-rinsed prior to pipetting, by drawing and expelling the liquid to be me­asured. This is especially important when wor­king with liquids of viscosities and densities different from that of water and in temperatu­res different from ambient temperature,
• when pipeting liquids which tend to wet walls of tips, such as serums, proteins or organic solvents, measuring must be performed much slower than with other liquids,
tips must be replaced with new ones, when
• changing from one liquid to another, or if drops of liquid remain inside the tips,
• after work, the pipette should be stored verti­cally in a holder with tips removed.
5 - RECALIBRATION
BioPette™
method, using
pipettes are calibrated by gravimetric
Labnet
tips and distilled water, at the temperature 20±1°C, according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy error (the difference between the real aspirated volume and the preset volume) exceeds the permissible value given in the table in section 1, the pipette recalibration procedure should be carried out.
Before starting the recalibration it is necessary
5
to check whether the following requirements have been fulfilled during error determination:
• the ambient temperature, and the temperature of the pipette, tips and water was identical
• the density of the liquid used is close to that of distilled water
a balance with appropriate sensitivity has been
• used
Volume checked
0.1 - 10
10 - 100
> 100
[µl]
Balance sensitivity
0.001
0.01
0.1
[mg]
• mg/µl conversion factor has been taken into account
•the requirements given in sections 3 and 4 have been fulfilled
If the above conditions are satisfied and the accu­racy error for selected volume given in section 1 exceeds the permissible value, the pipette recalibration procedure should be carried out.
The recalibration can be performed within one full turn of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the pipette, tips and liquid should be within the range 20-25°C and stabilized during weigh-
within ±0.5°C
ing
•Measurements should be conducted using distilled water
• Balance sensitivity should be suitable for the volume to be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette volume according to the following table:
Range of the pipette volumes [µl]
1 - 10
5 - 50
20 - 200
50 - 300
Preset
volume
[µl]
1
5
20
50
Permissible
volumes
[µl]
0.92 - 1.08
4.8 - 5.2
19.6 - 20.4
49.2 - 50.8
Volume change V for full turn of the calibra-
tion key [µl]
(24 increments)
0.33
1.67
6.30
10.00
6
MULTICHANNEL
•Perform three aspiration series (each series should include the aspirations from all chan­nels), weigh each time and calculate the aver­age value of the aspirations.
• Calculate average aspirated volume in µl mul­tiplying the average aspiration amount [mg] by the distilled water density coefficient [µl/mg], which depends on temperature and pressure according to the following table:
Temperature [°C]
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040
Pressure [kPa]
95 101.3 105
If the average aspirated volume exceeds the permissible value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A)
Warning: The pipetting pushbutton consists of 2 parts: the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After removal of the pushbutton, both parts are separated.
•Holding the volume setting knob to protect it against rotation, insert the calibration key into the cuts of the calibration screw, (Fig. 4B)
• Turn the key clockwise to reduce the aspirat­ed volume, or counter-clockwise to increase the volume, (Fig. 4C). One full turn of the calibra­tion key changes the pipette aspiration volume by the amount given in the table
• Take out the key and fix the pipetting pushbut­ton (Fig. 4D). The pipetting pushbutton should be fixed by placing the knob on the arbor first (Fig. 1A2) and then the pushbutton (Fig. 1A1).
Determine the average aspirated volume. The average volume should be within the permissi­ble range given in the table. If the volume exceeds the values stated, the recalibration pro­cedure should be repeated.
6 - CLEANING AND STERILIZATION
Cleaning
External surfaces of the pipetting pushbutton, the ejector button, the handgrip, shaft and the adjustment knob may be cleaned using a cloth dampened in isopropyl alcohol.
Sterilization:
The pipette can be sterilized in the autoclave at 121 °C for 20 minutes. After sterilization, the pi­pette should be dried and cooled to room tem­perature.
It is recommended:
- to sterilize the pipette in autoclave with an initial vacuum and drying cycle,
- prior to sterilization unscrew the nut connecting the handle and module slightly. After autoclav­ing these parts should be screwed tight again.
The precision of the results should not alter if the pipetting process and autoclaving are carried out as described in this manual. Because a slight change in the accuracy of the dosage may occur, it is recommended to:
- check the calibration of the pipette after the ini­tial first, third and fifth autoclaving cycles and then after every 10 autoclaving cycles.
7 - PIPETTE KIT AND ACCESSORIES
Pipette kit
The pipettes are delivered in the kits including:
• Pipette
• Instruction manual
• Calibration key
• Identification labels
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MULTICHANNEL
Accessories
Pipettor Tip Choices
BPE-10
BPE-50 BPE-200 BPT-50 BPT-200
BPE-300 BPT-300
Pipettor Stand Choices
BPE
BPT
P 4803-10, 10 µl tips, racked
P 4803-200, 200 µl tips, racked
P 4803-300, 300 µl tips, racked
P 5421 Stand for eight channel pipette
P 5422 Stand for twelve channel pipette
8 - SPARE PARTS
The spare parts for multichannel, (Fig. 1, 4):
A: Pipetting pushbutton A1: Pushbutton A2: Knob B: Adjustment knob C: Ejector button D: Piston’s assembly E: Shaft F: Ejector G: Calibration key
Can be ordered from a Labnet representative (type of the pipette and name of the part for this pipette should be specified).
Warning: The replacement of the plunger requires conducting of calibration proce­dure according to section 5.
Before returning a pipette to a Labnet representa­tive please ensure that the pipette is completely free of any contamination (chemical, microbial or radioactive).
Contact Labnet at 732-417-0700, fax: 732-417-1750, email: labnet@labnetlink.com
BPM/08/2004
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