Labnet Incubator Shaker
I5311-DS and I-5311-DS-230V
Instruction Manual
Copyright July 2004
PO Box 841 Woodbridge, NJ 07095 USA
Phone: 732-417-0700 Fax: 732-417-1750
www.labnetlink.com
CONTENTS OF THIS USERS MANUAL
Graphic Symbols page 1 Controls & Calibration page 3
Safety Precautions page 1 Operating the I-5311-DS page 5
Introduction page 2 Care and Maintenance page 5
Specifications page 2 Troubleshooting Guide page 6
Unpacking page 2 Technical Support / Service page 6
Installation page 3 Accessories page 7
GRAPHIC SYMBOLS
Y our Labnet I-531 1-DS incubator shaker uses internationally accepted graphic symbols to
help convey information to the user and to call the users attention to important safety precautions and guides for using this equipment.
indicates that user should consult manual further description or discussion.
Indicates AC “Power On” Indicates “Heating”
Indicates “Degrees Centigrade” Indicates “Temperature”
Indicates Potential “Shock Hazard” Indicates Protective “Earthground”
SAFETY PRECAUTIONS
Use of this product in any manner not specified by the manufacturer may impair the
safety protection provided by the equipment and may result in physical damage and/
or personal injury . Please read all operating instructions in this manual prior to use of
this equipment.
1. Do not operate this unit in an explosive or flammable environment.
2. Do not incubate or shake flammable or explosive materials or highly reactive chemicals.
3. Lifting / Handling: These units are heavy and care should be taken to use appropriate
lifting devices. Units should only be lifted from their bottom surfaces and not by doors,
handles or knobs.
4. Leveling: The unit must sit level and solidly on the four leveling feet.
5. Load testing: T est all loads to be shaken under observation to insure load and unit
stability.
6. Units are not stackable. Do not stack one unit on top of another unit.
INTRODUCTION
This manual covers the specifications, operation and use of the Labnet I-531 1-DS shaking incubator and its accessories. Please pay special attention to the Safety Precautions section in this
manual.
The I-531 1-DS shaking incubator from Labnet International provides an extremely stable temperature environment, and an aggressive shaking motion for the mixing of materials for cell culture
growth at ambient and above ambient temperatures.
The I-531 1-DS shaking incubator uses microprocessor controls and mechanical convection to
maintain a stable temperature environment and to achieve fast chamber temperature recovery
after a door opening. The incubator interior is constructed from stainless steel for corrosion resistance and easy clean-up of accidental spills. All incubator doors have an integral glass window and
the incubator has a light to allow observation of samples without opening the door . Each door is
fully thermal gasketed.
The I-531 1-DS shaking incubator comes with one removable full shelf and four large, rubber padded adjustable feet for leveling the unit. Additional half and full shelves may be purchased. The I531 1-DS shaking mechanism supports a wide range of optional rack and flask platforms which are
user changeable.
SPECIFICATIONS
Chamber temperature range Ambient +5C to 70C (Ambient +10°C with shaker on)
Accuracy / Uniformity @37°C +/-0.3C / +/-0.5C
Temperature accuracy +/-0.2C
T emperature Controller Microprocessor - User Calibratable
Over temperature safety Independently settable
Shaker speed 20 to 300 rpm
Shaker orbit 19mm
Shaker max load 7.4kg*
Shaker timer Continuous (Hld) or 30 seconds to 99 minutes 50 seconds
Timer increments 30 seconds to 9 min 59 seconds in 1 second steps
10.0 minutes to 99 min 50 seconds in 10 second increments
Interior electrical outlet 1 amp
Chamber volume 2.5 cu ft / 70 liters
Exterior dimensions w x d x h 574 x 544 x 644mm
Chamber dimensions w x d x h 442 x 396 x 406mm
Electrical Ratings and Unit Size
I-531 1-DS 120V , 50/60Hz, 6 amp s
I-531 1-DS-230V 230V, 50/60Hz, 4 amps
* Max load is 4 x 2 liter flasks with 400ml liquid in each flask
UNPACKING
Upon receipt of your I-531 1-DS shaking incubator, examine the carton and unit for damages. If shipping
damage has occurred, a claim must be filed with the carrier. The carrier is responsible for correcting
shipping damages. Save all packaging until the unit has been shown to operate properly to
your satisfaction. Carefully remove the unit from the carton and shipping pallet.
The package should include:
I-531 1-DS Shaking Incubator Instruction manual
1 full shelf Power cord (both EU and UK cords in
2 shelf brackets 230V models)
4 adjustable feet Warranty Card
Complete and return the warranty card to register your new I-531 1-DS Shaking Incubator
INSTALLATION
Install the four adjustable feet and locate Shaking Incubator on a stable, flat, very solid surface
near a grounded electrical outlet. The location selected should be out of direct sunlight and
away from heat producing sources or hot or cold air drafts. At least 5cm ventilation clearance
is required around all sides of the incubator. Level the incubator using the four adjust able feet.
Clockwise rotation of a foot raises the incubator . Plug in the unit to a properly rated and
grounded electrical outlet and the unit will be ready for use. Install the optional platform you
have selected by plugging in the four bottom protrusions. Larger flask platforms may require
being screw clamped to the shaking mechanism.
The shelf may be installed when shaking flasks 1 liter or smaller . However, the shelf severely
limits the illumination from the chamber light and the shelf may possibly rattle under aggressive
shaking conditions.
CONTROLS & CALIBRATION
The controls for the I531 1-DS Shaker Incubator include power to the oven, temperature setting,
safety temperature overshoot setting and shaker speed and shaking time. In addition there is
a door-open shaker shut-off switch and a chamber light switch.
- Main Power Switch - This switch (illuminated GREEN when on) turns the power to the unit
On and Off.
-T emperature Controller and T emperature Set – The controller has a 3-digit display for
displaying chamber temperature or set point information. UP and Down arrow pads are used
to change the set point and controller mode of operation. T o enter the set point mode of
operation, press either the UP or DOWN arrow pad one time. The display will start to blink,
going from bright to dim. While blinking, the display is showing the set point. T o change the set
point, use the UP and DOWN arrow pads. If the arrow pads are not pressed for five (5) seconds, the display will stop blinking and will read the chamber temperature. After setting temperature, allow at least one hour for the chamber temperature to stabilize and 24 hours for
optimum stabilization.
-Calibration- The Shaking Incubator is calibrated at 37C at the factory . The unit can be recalibrated after the chamber temperature has stabilized at the set point for several hours.
Suspend a certified reference thermometer in the chamber . Compare the units display to the
reference thermometer. If there is an unaccept able difference, put the controller into calibration
mode by pressing both the UP and DOWN arrow pads at the same time until the two outside
decimal points begin to flash. While the decimal points are flashing, the display can be calibrated to match the reference thermometer by pressing the UP or DOWN arrow pads until the
display reads the correct value. Allow the shaker incubator to st abilize again, and recalibrate if
necessary .
-Heating Indicator-This indicator will illuminate GREEN when the controller is calling for heat
from the heater. This indicator will be on continuously while the oven heats up to the set temperature and will then cycle on and off at the set temperature.
-Safety Thermostat-The safety thermostat located on the back of the unit is manually set and
completely independant of the Main T ermperature Controller . The safety gurads against any
failure of the Main Controller that would allow temperature to rise past the safety set point. If
the temperature rises to the safety set point, the Safety takes control of the heating element
and allows continued use of the incubator until the problem can be resolved or service can be
arranged.
-Safety Indicator-This indicator wil illimunate RED when the Safety Thermostat is activated.
Under normal operating conditions this indicator should never be on.
-Chamber Light Switch-This switch turns the chamber light on and off when power is on.
-SHAKER CONTROLS-The shaker controls are also microprocessor based.
-RUN lamp-This lamp illuminates when the shaker is started and stays illuminated when the
shaker is running.
-Timer lamp-This lamp is illuminated when the shaker display is in the time display mode
counting down the remaining run time or showing the set run time.
-RPM lamp-This lamp illuminates when the shaker display is speed display mode showing the
current shaking speed or showing the set shaking speed.
-Stop / Start-This pad starts and stops the shaking mechanism and causes the RUN lamp to
be illuminated.
-Push to Shift / T urn Set Knob- This encoder provides the following multiple functions:
• It increases and decreases the shaker speed setting in 1 rpm increments from 20 rpm
to 300 rpm.
• It increases and decreases the run time setting from 30 seconds to 99 minutes and 50
seconds and HOLD (Hld) for continuous operation. Time is changed in 1 second
increments to 10 minutes and then 10 second increments thereafter .
• It shifts the shaker display from time display to speed display and back
-Shaker Display- This three digit display shows the shaker speed setting or shaker time
setting or remaining shaker run time while counting down. It also shows:
• Hld - When the shaker is set for continuous un-timed running
• Opn - When the incubator door has been opened, automatically stopping the shaking
motion (note the timer continues to count down but is not displayed)
• End - When a timed shaking run has ended
• F60 / F50 - When the shaking incubator is first powered up indicating the line
frequency of the power line.
-Door Open Switch - This switch senses when the incubator shaker door is open and sends
a signal to the microprocessor which causes the shaker to stop running as a safety measure.
However, in a timed run, the timer will continue to count down when the door is open. Closing
the door restarts the shaking motion.
OPERATING THE LABNET I-5311-DS INCUBATOR SHAKER
As advised in the installation section, it is very important that the unit be level and placed on a
very solid surface. When loading the platforms, it is also important to load these in a balanced
manner or the unit can vibrate and possibly walk. Shaking unbalanced loads for extensive
periods may also damage the shaker.
Before powering up the unit, you should first set the mechanical Safety Thermostat on the back
of the unit to a temperature just above your intended operating temperature or at max (setting
of 10) this safety is not needed.
After you first power up the unit you should notice the shaker display first showing dashes then
the power input line frequency (F60 or F50 for 60 Hz or 50Hz) and the temperature controller
display showing some random numbers and dots blinking.
The shaker display and temperature display should then settle down with the shaker display
showing the last time that was set (or HLd) for continuous operation, and the temperature
display showing the chamber temperature.
Y ou may now set the desired operating temperature, the desired shaking speed and the
desired shaking time (see Controls section of this manual). It is recommended that you test all
loads to be shaken a slow speed under observation and then gradually raise the speed to
insure the load and unit are stable. The unit should also be allowed to equilibrate to the set
temperature before using.
Once the temperature, shaking speed and shaking time are set, use the Stop / S tart pad to
initiate shaking. When shaking in the timed mode, the word End will be displayed on the
shaker display when the shaking is done. In timed mode the word Hld (hold) will be displayed
on the shaker display . The user may switch the shaker display to show the shaking speed by
pressing the “Push to Shift” knob.
The user can observe the load being shaken through the glass window and can also turn on
the chamber light for additional visibility . The chamber light should not be left on for an
extended period as it adds heat to the chamber and may cause the chamber temperature to rise above the set point.
The user may open the chamber door at any time to gain access the chamber . If the shaker is
running when the door is open, the shaker power will be immediately cut causing the shaker to
slow and stop. The shaker display will show Opn for open. Shaking will automatically resume
when the door is closed if there is still time remaining on the shaker run. Note: The clock
continues to count down when the door is open even though the shaker has stopped.
CARE & MAINTENANCE
No routine maintenance is requires for the electrical or mechanical components of the unit. The
incubator exterior , interior and shaking platform should be wiped down periodically with a soft
damp cloth with mild soap. Do not use chlorine-based bleach or abrasives. Any spills in the
incubator and/or on the shaking platforms should be cleaned up immediately . Be sure to
disconnect the power cord before cleaning or decontaminating the oven.
TROUBLESHOOTING GUIDE
PROBLEM POSSIBLE SOLUTION
Oven will not power up or not heat Check power cord, outlet and unit circuit breaker
Shaking platform will not operate Check timer set to Hld or a time setting
Check that nothing is blocking shaker platform
Press shaker STOP/ST ART button
T emperature too high Check set point and readjust if necessary
Check calibration
Chamber temperature goes above set Normal operation in initial heat up or if door
point and settles back to set point opened for a long period
T emperature will not remain stable Check that set point is at least 5C above ambient
or display shows “LO” (or 10°C above ambient when shaker is on) which is
minimum operating point
Indicated temperature is unstable A slight variation of +/-0.1C is normal. Larger fluctua-
tions may be ambient variations from drafts, door
opening and closing, a fan obstruction or failure or
electrical noise from RFI (motors, etc.)
T emperature is too low If door has opened, unit may not have recovered yet.
Confirm temperature set point.
Safety thermostat set too low .
Unit will not heat above temperature Confirm set point. Check temperature of chamber
that is below set point with a thermometer and recalibrate if needed.
T emperature display and reference Be sure that unit has been allowed to stabilize for
thermometer do not match 1 hour. Thermometer should be suspended in
the chamber and not touching any surface. Only
certified reference thermometers should be used.
Cannot adjust set point or calibration Turn unit off for 5 seconds to reset. If problem
or display persists call service persists, call for service.
Unit calibrated at one temperature This can be a normal condition if temperatures or load
and not at another vary widely . For best accuracy , calibrate at set point
TECHNICAL SUPPORT / SERVICE
Should your I-531 1-DS shaking incubator require service, call Labnet International’s Service
Department at 732-417-0700 between the hours of 9:00am and 5:00pm EST . If service
necessitates the return of the unit to Labnet’s Service Department, a return authorization (RA)
number must first be obtained and a decontamination certificate completed. The unit must be
thoroughly decontaminated before shipping. The unit must then be sufficiently packed for
shipment. Any shipping damage on returned unit s will be the customer’s responsibility .
ACCESSORIES
I-5322 Full shelf w / brackets
I-5323 Half shelf w / brackets
I-5330 Universal flask clamp platform
I-5331 Flat mat platform
I-5330-50 30 x 50ml flasks platform
I-5330-125 20 x 125ml flasks platform
I-5330-250 12 x 250ml flasks platform
I-5330-500 8 x 500ml flasks platform
I-5330-1000 6 x 1000ml flasks platform
I-5330-2000 4 x 2000ml flasks platform
Flask clamps are available in 50ml, 125ml, 150ml, 500ml, 1000ml, and 2000ml.
T est tube rack clamps are also available.
Labnet is continuously adding accessories. Check with your representative or call Labnet directly
at 732-417-0700 or check our website: www.labnetlink.com for a complete listing of accessories
and flask clamps.
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