The information contained in this manual and the accompanying products are copyrighted and all
rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic
design changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
•Purifier® Logic® Biological Safety Cabinets and PuriCare® Lab Animal Research Stations
carry a three-year warranty from date of installation or four years from date of shipment from
Labconco, whichever is sooner.
•SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date
of installation or three years from date of shipment from Labconco, whichever is sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight
carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the
delivery carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed
by various federal, state, or local regulations. All users of this equipment are required to become
familiar with any regulations that apply in the user’s area concerning the dumping of waste materials
in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held
harmless with respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #5233703, Rev. B
ECO G978
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: PREREQUISITES 2
Support, Vibration & Preventive Requirements 3
Location Requirements 3
Exhaust and Blower Requirements 3
ISO Class 3 Definition 5
Electrical Requirements 7
Space Requirements 7
CHAPTER 3: GETTING STARTED 8
Unpacking the Glove Box 9
Installing the Glove Box on a Supporting Structure &
Work Surface 10
Connecting to the Exhaust System (If Applicable) 10
Installation of Gloves to the Glove Ports 12
Connecting the Electrical Supply to the Glove Box 13
Setting Exhaust Volume with Speed Control Adjustment 13
Validating the Glove Box 14
Summary of Performance Testing Results 14
CHAPTER 4: PERFORMANCE FEATURES AND
SAFETY PRECAUTIONS 16
Performance Features 16
Safety Precautions 19
CHAPTER 5: USING YOUR GLOVE BOX 21 Routine Daily Work Procedures 21
HEPA Filter Applications, Suitability & Guidelines 23
Definition of Terms 24
Appropriate Chemicals for Carbon Filters 24
Hazardous Misapplications for Carbon Filters with
Volatile Chemicals 25
Chemical Carcinogen Use with Carbon Filters 25
Prohibited Acid Use 26
O
O
FF
C
C
OONNTTEENNTTSS
CHAPTER 6: MAINTAINING YOUR GLOVE BOX 27
Routine Maintenance Schedule 28
Decontamination 29
Determining When to Replace HEPA Filters 29
How to Install New HEPA Filters 29
HEPA Filter Leak Test 31
Initial Certification 33
Re-Certification 33
Fluorescent Light Replacement 33
Motorized Impeller Replacement 34
Speed Control Replacement 34
Ionizer Fan Maintenance 34
CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 35
CHAPTER 8: TROUBLESHOOTING 42
APPENDIX A: REPLACEMENT PARTS 43
APPENDIX B: DIMENSIONS 45
APPENDIX C: SPECIFICATIONS 46
APPENDIX D: IONIZER FAN PERFORMANCE CRITERIA 47
APPENDIX E: PRESSURE & FLOW RATE CONVERSIONS 49
APPENDIX F: REFERENCES 50
DECLARATION OF CONFORMITY 51
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco XPert® Weigh Box. Your
glove box provides personnel protection through superior containment rated
at Class 1 conditions for hazardous environments, per ISO 10648-2. The
XPert Weigh Box has two added features that provide an added benefit
during weighing procedures. These two features include the stainless steel
covered marble slab and the anti-static ionizer fan.
These glove boxes will effectively contain toxic or noxious particulates when
properly installed and operated. In positive or negative pressure operation
modes, the glove box can create an ISO standard Class 3 (ISO 14644-1) clean
air environment. This glove box uses two HEPA filters, rated at least 99.99%
efficient for 0.3-micron particles.
The XPert Weigh Boxes offer many unique features to enhance safety and
performance. To take full advantage of them, please acquaint yourself with
this manual and keep it handy for future reference. All glove box operators
must read and understand how HEPA filtered glove boxes operate; please
review Chapter 4: Performance Features and Safety Precautions before
beginning to work with this glove box. Even if you are an experienced user,
please review Chapter 5: Using Your XPert Weigh Box, which describes the
XPert features so that you can use the filtered glove box effectively and
safely.
Product Service 1-800-522-7658
1
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install the glove box, you need to prepare your site for
installation. You must be certain that the area is level and of solid
construction. In addition, a dedicated source of electrical power should be
located near the installation site to power the glove box. The glove box
should be strategically placed in the lab to provide efficient workflow and
prevent operator interference from normal traffic patterns.
Carefully read this chapter to learn the requirements for your installation site:
• The support, vibration and preventive requirements.
• The location requirements.
• The exhaust and blower requirements.
• The electrical power requirements.
• The space requirements.
Refer to Appendix B: XPert Weigh Box Dimensions for complete glove box
dimensions.
Refer to Appendix C:XPert Weigh Box Specifications for complete electrical
and environmental conditions, specifications and requirements.
2
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Support, Vibration and Preventive Requirements
In the preparation of a glove box site, please consider the following:
•A bench or stand that is rigidly mounted to the floor or fixed to the wall,
but not both, may be appropriate. 35" to 40" (889mm-1016mm) is
typical for standing height. (Labconco stands offered in Chapter 7 vary
in height from 33" to 40".)
•The corners of a building typically have less vibration than the center,
which promotes analytical balance stability.
•The bench typically should not contain any vibration-producing
equipment, such as shakers or pumps.
•A marble slab with dampening pads placed within the enclosure is an
effective low cost means of controlling vibration and one is included
with the XPert Weigh Box.
Location Requirements
The XPert Weigh Boxes have been designed to rest on a typical 29"-30"
(737mm-762mm) deep work surface. The height should be 35"-40" for
standing position. Avoid placing the glove box in high traffic areas where
walking might disrupt the operator or experimentation.
Exhaust and Blower Requirements
The XPert Weigh Box, as manufactured, uses an integral motorized impeller
to pull room air through the inlet and exhaust HEPA filters and discharge the
clean air back into the laboratory. This negative pressure operation mode
pulls all internally-contaminated air through the exhaust HEPA filter. The
HEPA-filtered exhaust air is then forced out the top of the glove box. An
optional FilterMate™ Portable Exhauster with a carbon filter may be
installed downstream of the exhaust to adsorb low levels of organic fumes,
formaldehyde, or ammonia gases.
The HEPA-filtered exhaust air from the glove box can be exhausted to the
outside with the installation of a thimble exhaust connection kit and remote
blower listed in Chapter 7. As an option, if necessary, the built-in motorized
impeller can be wall mounted.
Labconco does not recommend a hard duct connection using the built-in
motorized impeller as the source of airflow out of the facility. This
creates a positive pressure in the exhaust duct.
Product Service 1-800-522-7658
3
Chapter 2: Prerequisites
Labconco does not recommend hard duct connections to remote blowers
without a thimble due to the air volume balance requirements with such
systems. Hard duct connections should only be used when the glove box
impeller is disconnected and only a single roof-mounted blower is used.
Note that reliance on an external exhaust blower does not provide
airflow control at the glove box.
If a positive internal pressure clean air ISO Class 3 condition (ISO 14644-1)
is desired, the motorized impeller can be rotated to work in positive pressure
mode (see Figure 3-3). See Chapter 7 for the Positive Pressure Conversion
Kit. See the following Negative and Positive Pressure Particle Test data in
the charts below. Both charts show the dilution rates of the glove boxes.
500
450
400
350
300
(Per Cubic Meter Per Minute)
250
200
150
100
Number of Particles
NOTE: Ambient Air Baseline Particle Count at 1 Minute
294132 Particles measuring 0.3 Micron detected within 1 Minute.
33296 Particles measuring 0.5 Micron detected within 1 Minute.
Airflow Testing Performed at 50 Dial Setting (61 CFM)
with Glove Box Under Negative Pressure.
> 0.3 Micron
> 0.5 Micron
50
0
0
0
012345678910111213141516171819
60
24
14
1111
13
11
12
1111
0000000
9
6
4
Time (Minutes)
XPert Weigh Box Particle Testing under Negative Pressure
0
4
Product Service 1-800-522-7658
Chapter 2: Prerequisites
500
NOTE: Ambient Air Baseline Particle Count at 1 Minute
450
198671 Particles measuring 0.3 Mi cron detected within 1 Minute.
25473 Particles measuring 0.5 Micron detected within 1 Minute.
400
350
300
(Per Cubic Meter Per Minute)
250
200
150
100
Number of Particles
50
0
0
0123456
306
Airflow Testing Performed at 50 Dial Setting (61 CFM)
with Glove Box Under Positive Pressure.
139
> 0.3 Micron
> 0.5 Micron
18
12
2
8
1
0
00
Time (Minutes)
XPert Weigh Box Particle Testing under Positive Pressure
ISO Class 3 Definition
Airborne particulate cleanliness inside any clean air glove box or enclosure is
designated by ISO Class 3, which is equivalent to 35 particles 0.5 µm or larger
per cubic meter of air per minute as defined by ISO Standard 14644-1. ISO
Class 3 cleanliness is illustrated in the table below and is equivalent to Class 1
air conditions as defined by Federal Standard 209E or 1 particle 0.5 µm or
larger per cubic foot of air per minute.
Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.
ISO
classification
number (N)
ISO Class 1 10 2
ISO Class 2 100 24 10 4
ISO Class 3 1 000 237 102 35 8
ISO Class 4 10 000 2 370 1 020 352 83
ISO Class 5 100 000 23 700 10 200 3 520 832 29
ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293
ISO Class 7 352 000 83 200 2 930
Maximum concentration limits (particles/m3 of air/minute) for particles equal to
and larger than the considered sizes shown below (concentration limits are
calculated in accordance with 3.2 of Standard 14644-1)
0.1 µm 0.2 µm 0.3 µm 0.5 µm 1 µm 5 µm
ISO Class 8 3 520 000 832 000 29 300
ISO Class 9 35 200 000 8 320 000 293 000
Table 2-1 ISO Classification Number (N)
Product Service 1-800-522-7658
5
Dial Setting
Min. Dial
20 Dial
40 Dial
50 Dial
60 Dial
80 Dial
Max. 100 Dial
Dial OFF
Chapter 2: Prerequisites
Additionally, the motorized impeller can also be used in a full recirculation
mode providing the operator with some control of the atmosphere chemistry
within the glove box. See Chapter 7 for the Recirculation Conversion Kit.
The airflow chart below represents typical operational data including air
changes per minute, exhaust volume, noise levels, main chamber static
pressures, and total system static pressures measured at the blower intake
(suction side). Exhaust volumes are reduced by 17% for products operating
on 100V, 60 Hz and reduced 34% for 100V, 50 Hz products.
Air Changes
per Minute
(acm)
1
1.5
3.3
4.7
6.2
7.2
7.7
0
Exhaust
Volume
(CFM)
13
20
43
61
80
93
100
0
Noise Pressure
dB (A)
44
48
48
65
68
70
71
37
Static Pressure
Inside Glove Box
(inches of water)
.14
.17
.45
.68
.80
.89
.91
0
Total System
Static Pressure
(inches of water)
.34
.51
1.32
1.81
2.14
2.38
2.42
0
XPERT WEIGH BOX
2.5
Static Pressure Inside Glove Box
2
1.5
1
(Inches of Water Column)
Negative Static Pressure
0.5
0
102030405060708090100
Total Static Pressure at Blower
Intake (Suction Side)
0.5072
0.3435
0.17
0.14
Static Pressure Test Results
1.81
1.323
0.68
0.45
Airflow Volume
(Cubic Feet per Minute)
2.141
0.8
2.375
0.89
2.422
0.91
6
Product Service 1-800-522-7658
!
Chapter 2: Prerequisites
For thimble-ducted glove boxes, the proper remote blower selection can be
determined from these exhaust requirements as the remote blower only needs
to be sized for the static pressure loss of the ductwork system. The thimble
ducted exhaust blower should be sized for 25% additional airflow. When
exhausting a glove box to the outside, it should be connected to a dedicated
remote blower.
Labconco offers accessory remote blowers listed in Chapter 7. Contact
Labconco for blower sizing assistance.
When a glove box is connected to a remote blower exhaust system, an
adjustable damper (or valve) should be installed in the duct to control the
airflow volumes properly. See Chapter 7 for Exhaust Dampers.
Electrical Requirements
Electrical services should be nearby for connecting the glove box, and
accessory equipment. The glove box is rated 115V, 8A or 230V, 4A or
100V, 8A. A convenience duplex outlet is located inside the glove box and is
rated for 115V, 5A or 230V, 2A or 100V, 5A. A four outlet power strip is
included with the 115V XPert Weigh Box for connection to the duplex outlet.
International outlet strips are listed in Chapter 7.
Space Requirements
The dimensions are shown in Appendix B: Dimensions. Provide adequate
space to the right of the transfer chamber for loading and unloading.
Product Service 1-800-522-7658
7
CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Once the site for your XPert Weigh Box is properly prepared, you are ready
to unpack, inspect, install, and validate your system. Read this chapter to
learn how to:
• Unpack and move the glove box.
• Set up the glove box with the proper supporting structure and work
surface.
• Connect to an exhaust system if applicable.
• Install the gloves.
• Connect the electrical supply.
• Set the exhaust volume with the speed control adjustment.
• Arrange validation for the glove box.
Each XPert Glove Box weighs 275 lbs. (124 kg). The
!
shipping container allows for lifting with a mechanical lift
truck or floor jack. If you must lift the enclosure
manually, follow safe-lifting guidelines. Do not lift by the
upper sheet metal façade and front panel as damage can
occur to the plastic glove box liner (see Figure 3-1).
8
Product Service 1-800-522-7658
Chapter 3: Getting Started
Figure 3-1
Lifting Instructions
Unpacking the Glove Box
We recommend that you do not remove the glove box from its shipping
container until it is ready to be placed into its final location. Note: The
vibration isolating marble slab is packaged in a separate cardboard
carton attached to the main cardboard carton. Move the unit by placing a
flat, low dolly under the shipping skid, or by using a floor jack.
Carefully remove the carton shrink-wrap on the glove box and inspect it for
damage that may have occurred in transit. If damaged, notify the delivery
carrier immediately and retain the entire shipment intact for inspection
by the carrier.
THE UNITED STATES INTERSTATE COMMERCE
COMMISSION RULES REQUIRE THAT CLAIMS BE
)
)
FILED WITH THE DELIVERY CARRIER WITHIN
FIFTEEN (15) DAYS OF DELIVERY.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
Product Service 1-800-522-7658
9
Chapter 3: Getting Started
IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU
MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT
)
Do not discard the packing material until you have checked all of the
components and tested the glove box.
CARRIER. LABCONCO CORPORATION AND ITS
DEALERS ARE NOT RESPONSIBLE FOR SHIPPING
DAMAGES.
Installing the Glove Box on a Supporting
Structure and Work Surface
Exercise caution when lifting or moving the glove box.
When installing the glove box onto a work surface or benchtop, ensure that
the structure can safely support the combined weight of the glove box and
any related equipment. The work surface should be as wide as the glove box
to properly support it. The front of the glove box should be aligned with the
front of the work surface for optimal comfort. A height of 35"-40" (889mm1016mm) is appropriate for standing operation. Adjustable Height Base
Stands listed in Chapter 7 may be adjusted from 33"-40" (838mm-1016mm).
Connecting to the Exhaust System (If Applicable)
WARNING: The weight of any exhaust ductwork system
!
The XPert Weigh Box has been manufactured to exhaust into the laboratory
in its standard configuration. For exhausting to the outside of the building
(see Figure 3-2), a Thimble Exhaust Connection should be ordered (see
Chapter 7). To remove chemical fumes and odors not trapped by the HEPA
filter, the glove box can be exhausted to the outside, or connected to an
accessory FilterMate Portable Exhauster with carbon filters (see Figure 3-2).
See Chapter 7 for accessories including: Thimble Exhaust Connection,
FilterMate Portable Exhauster, Positive Pressure Conversion Kit,
Recirucation Kit, Hard Duct Connection.
Consult Labconco Customer Service if you require help sizing a remote
blower for the exhaust volume and the duct work system (static pressure loss
listed in Chapter 2).
must be supported independently of the glove box
superstructure or damage may occur.
The exhaust system should be installed by a qualified
HVAC contractor.
10
Product Service 1-800-522-7658
!
Chapter 3: Getting Started
To ensure compatibility, the selected exhaust duct material
should match the enclosure, procedures and chemical
applications.
Exhaust Connection to Outside
(All dimensions in inches)
Product Service 1-800-522-7658
Figure 3-2
11
Chapter 3: Getting Started
Positive Pressure Glove Box Configuration
Figure 3-3
Installation of Gloves to the Glove Ports
With thumbs up and right/left orientation, secure the gloves in place on the
glove ports by stretching the beaded cuff into the groove nearest the window.
Install the separate 8" diameter O-ring over the gloves and into the outer
groove of the glove port surface. Stainless steel band clamps are provided for
securing the separate O-ring into the glove port groove. Replacement gloves
and parts are listed in Appendix A with a decription in Chapter 7.
12
Figure 3-4
Glove Installation
Product Service 1-800-522-7658
Chapter 3: Getting Started
Connecting the Electrical Supply to the Glove Box
115V, 60 Hz; 100V, 50 Hz; or 100V, 60 Hz Models
Connect the power cord supplied to the IEC electrical supply plug on the
back of the glove box. 115V or 100V models are rated at 8 amps total.
The maximum circuit load for the interior electrical duplex is 5 amps.
230V, 50 Hz or 230V, 60 Hz Models
The same procedure applies for the 230V model except it is shipped without
a plug. Install the appropriate plug per local requirements. The 230V, 50 Hz
or 230V, 60 Hz models are both rated at 4 amps total. The interior
electrical duplex is rated at 2 amps.
Setting the Exhaust Volume with the Speed
Control Adjustment
For XPert Weigh Boxes, adjustment of the speed control provides control of
exhaust air volume and is located on the front panel. The exhaust air volume
should be adjusted at the low setting for weighing operations. (Consult your Safety Officer for airflow recommendations for your application).
Working at the lowest exhaust volume appropriate for the application will
give the quietest operation. To increase the rate of air volume changes after
working in the glove box and to ensure proper cleaning, the speed control
should be set at a higher (50 to 100) dial setting for 5 to 10 minutes as
recommended by the operator’s predetermined protocol and the chart below.
The exhaust volume is increased by turning the speed control
counterclockwise.
Minimum
Dial Setting
for Cleaning
Internal Air
Volume
50 4.7 61 10
100 7.7 100 5
Approximate
Air Volume
Changes per
Minute
Estimated
Total Exhaust
Volume
(CFM)
Recommended
Times to
Clean Internal
Air Volume
(minutes)
Product Service 1-800-522-7658
13
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