Labconco Total Exhaust Biological Safety Cabinets User Manual
Use
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ua
TYPE
B2
Original instructions
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Purifier®Cell
M
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Total Exhaust Biological Safety
Cabinets
Models
32348 Series 33348 Series 34348 Series
32368 Series 33368 Series 34368 Series
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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com
Please read the User’s Manual before operating the equipment.
This page is intentionally blank.
Original instructions
Original instructions
g
Copyright © 2013 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
• Purifier® Logic
• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from
• Blood Drawing Chairs carry a ten-year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
®
+ Biological Safety Cabinets and PuriCare® Procedure Stations carry a
five-year warranty from date of installation or six years from date of shipment from
Labconco, whichever is sooner.
date of installation or three years from date of shipment from Labconco, whichever is sooner.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
e due to corrosion or accidental breakage is not covered.
Dama
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight
carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar
with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon
water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with
respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #4028911 Rev. B
ECO H728
Original instructions
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: PREREQUISITES 2
Space Requirements 2
Clearance 2
Location Requirements 3
Exhaust Requirements 4
Electrical Requirements 4-5
Service Line Requirements 5
User-Supplied Microscope Requirements –
With Scope-Ready
User-Supplied Microscope Requirements –
With Temp ZoneTM Package Only 6
CHAPTER 3: GETTING STARTED 7
Unpacking the Biosafety Cabinet 8
Preparing the Biosafety Cabinet for Operation 8
Moving and Lifting the Biosafety Cabinet 9
Installing the Biosafety Cabinet on an Existing Work Surface 9
Installing the Biosafety Cabinet on a Labconco Base Stand 9
Telescoping Base Stands 10
Manual or Electric Hydraulic Lift Base Stands 10
Connecting the Biosafety Cabinet to Utility Service Lines 11
Optional Vacu-Pass
Drain Valve Installation 13
Microscope Installation (optional) 14-15
Circulator Installation (optional) 16-17
Initial Certification 18
O
O
C
FF
TM
TM
Cord and Cable Portal Use 12
OONNTTEENNTTSS
C
Package Only 6
Original instructions
CHAPTER 4: PERFORMANCE FEATURES AND SAFETY
HEPA Filters 20
ULPA Filters 21
Laminar Airflow 21
Directional Airflow 22
Motor/Blower 23
Cabinet Air Intakes (Grilles), Ductwork & Air Balance Controls 24
Ultraviolet (UV) Lamp 24-25
Scope-ReadyTM Package Option 25
Temp-ZoneTM Package Option 26
Safety Precautions 26-27
CHAPTER 5: USING THE CABINET 28
System Reset Switch 28
Information Center 29
Alarm Screens 30
Operating the Sliding Sash 31
Starting the Biosafety Cabinet 31
The Logic+ Touchpad 32
Navigating the Logic+ Menu Screens 33
Navigating the MyLogicTM Menu Screens 34-36
Setting the Clock 34
Configuring the Logic+ 35-36
Navigating the Settings Screens 36-39
Units of Measure 36
Security Lock 37
RS-232 Output Rate 37
UV Settings 38-39
UV Lamp Hourmeter 38
Reset UV Lamp Hourmeter 38
Change UV Lamp Life 39
Service Menu Screens 39
Timer Operation 40
Interval Timer Operation 40
Stopwatch Timer Operation 40
If An Airflow Alert Activates 40
Resetting the Airflow Alert System 40
Working in the Biosafety Cabinet 41-43
PRECAUTIONS 19
Original instructions
CHAPTER 6: MAINTAINING THE CABINET 44
Routine Maintenance Schedule 44-45
Service Operations 45-49
Work Surface Removal 45
Front Grille Removal 46
Towel Catch Removal 47
Front Panel Removal & Installation 47-48
Changing the Fluorescent Lamps 48
Changing the Optional UV Lamp 48-49
Resetting a Circuit Breaker 49
Storage 49
CHAPTER 7: TROUBLESHOOTING 50-53
APPENDIX A: COMPONENTS 54-55
APPENDIX B: DIMENSIONS 56
APPENDIX C: SPECIFICATIONS 57-58
Electrical Data 57
Motor Specifications 57
Environmental Conditions 58
APPENDIX D: ACCESSORIES 59
APPENDIX E: QUICK CHART 60
APPENDIX F: INSTALLATION GUIDE FOR ALL PURE-VU
SEALS AND STAND-STILL ISOLATION PLATFORMS 61-69
DECLARATION OF CONFORMITY 70
CAUTION – See Manual. When this symbol is on the unit it
indicates a caution that is detailed in this manual.
ATTENTION - Voir manuel. Lorsque ce symbole est allumé
l'appareil, il indique une mise en garde qui est indiqué dans
ce manuel.
Original instructions
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on the purchase of a Labconco® Purifier Cell Logic+©
Biosafety Cabinet. The biosafety cabinet is designed to protect you, the
product and the laboratory environment from biohazardous aerosols. The Cell
Logic Series Cabinet is the result of users like you requiring a customer
supplied microscope integrated into the cabinet and a temperature controlled
work area to maintain sample temperature. The Cell Logic Biosafety Cabinet
is available with either or both Scope-Ready™ and Temp-Zone™ package
options.
This biosafety cabinet offers many unique features to enhance safety,
performance and ergonomics. To take full advantage of them, please acquaint
yourself with this manual and keep it handy for future reference. If you are
unfamiliar with how biosafety cabinets operate, please review Chapter 4:
Performance Features and Safety Precautions before you begin working in
the cabinet. Even if you are an experienced biosafety cabinet user, please
review Chapter 5: Using the Cabinet; it describes the biosafety cabinet’s
features so that you can use it efficiently.
This manual and other technical information is available in PDF format
at our website: www.labconco.com.
If the unit is not operated as specified in this manual it may impair the
protection provided by the unit.
Si l'unité n'est pas utilisée comme spécifié dans ce manuel il peut
diminuer la protection fournie par l'unité.
Product Service 1-800-522-7658
1
Original instructions
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install the Logic+, you need to prepare the site for installation.
Examine the location where you intend to install the cabinet. You must be
certain that the area is level and of solid construction. In addition, a dedicated
source of electrical power must be located near the installation site.
Carefully read this chapter to learn:
• Location requirements.
• Electrical power requirements.
• Exhaust requirements.
• Service utility requirements.
• Space requirements.
• User-supplied microscope requirements (if needed)
• User-supplied circulator requirements (if needed)
Refer to Appendix C: Specifications, for complete biosafety cabinet electrical
and environmental conditions, specifications and requirements.
Space Requirements
The dimensions for the Logic+ B2 biosafety cabinets are shown in Appendix B:
Dimensions.
Clearance
A minimum clearance of at least 6 inches (150 mm) is suggested on the top
and both sides of the cabinet for service.
2
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Original instructions
Location Requirements
Note: The biosafety cabinet should be located away from traffic patterns,
doors, fans, ventilation registers, fume hoods and any other air-handling
devices that could disrupt its airflow patterns. All windows in the room
should remain closed. Figure 2-1 shows the preferred location for the
biosafety cabinet.
Preferred location
Main Disconnect Device
(Power cord plug in back
of the electronics module).
Chapter 2: Prerequisites
Figure 2-1
Air register blocked or redirected
to prevent cabinet disruption
Alternate location
Do not position the unit so that it is difficult to operate the main
disconnect device.
Ne placez pas l'appareil de sorte qu'il est difficile de faire fonctionner le
dispositif principal de déconnexion.
Product Service 1-800-522-7658
3
Original instructions
Chapter 2: Prerequisites
Exhaust Requirements
NOTE: THE EXHAUST BLOWER CANNOT RUN AT ITS NOMINAL
FLOW RATE WITH THE SASH COMPLETELY CLOSED. IF YOU
CHOOSE TO CLOSE THE SASH COMPLETELY TO USE THE UV
LIGHT FOR SURFACE DISINFECTION, THEN THE EXHAUST
BLOWER MUST BE SHUT OFF, OR ITS FLOW RATE REDUCED 90%
OR MORE FOR PROPER OPERATION. THE LOGIC+ HAS AN
OPTIONAL EN/CONTACT CIRCUIT BOARD THAT HAS DRY
CONTACTS THAT CAN BE CONFIGURED TO SIGNAL THE
EXHAUST BLOWER TO TURN ON WHEN THE LOGIC+’s BLOWER IS
ON. FOR FURTHER INFORMATION, CONTACT LABCONCO’S
PRODUCT SERVICE DEPARTMENT. AS AN ALTERNATIVE, A
REMOTE ELECTRICAL SWITCH FOR THE EXHAUST BLOWER CAN
BE INSTALLED NEAR THE LOGIC+.
Before deciding on a location, examine it carefully to ensure that it
accommodates the cabinet’s exhaust duct. The area directly above the
cabinet’s exhaust port should be clear of structural elements, water and utility
lines, or other fixed obstructions. There should be enough clearance to allow
for the passage of a 10" stainless steel duct. Avoid cabinet locations that
require either an elbow directly on top of the cabinet’s exhaust connection or
an excessive number of elbows to clear other items. For a further discussion
of the cabinet’s exhaust system requirements, please go to Chapter 3: Getting
Started.
Electrical Requirements
The biosafety cabinet models have the following electrical requirements:
Model # Requirements
3x34xxx0x 115 VAC, 60 Hz, 12 Amps
3x34xxx1x 100 VAC, 50/60 Hz, 12 Amps
3x34xxx-20, 30, 40, 50, 60, 70 230 VAC, 50/60 Hz, 6 Amps
3x36xxx0x 115 VAC, 60 Hz, 12 Amps
3x36xxx1x 100 VAC, 50/60 Hz, 12 Amps
3x36xxx-20, 30, 40, 50, 60, 70 230 VAC, 50/60 Hz, 8 Amps
Note: A dedicated outlet with an appropriate circuit breaker should be
located as close as possible to the right rear side of the cabinet, at a
height even with, or higher than, the top of the bench or stand. Consult
your local electrical codes for properly rated circuit breakers. For safe
operation the dedicated outlet must provide the protective earthing
ground connection to the cabinet.
Table 2-1
4
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Original instructions
Note: On 100 and 115 VAC models, both electrical outlets are protected by a
ground fault interrupter circuit (GFIC). Labconco does not recommend
plugging the biosafety cabinet into a GFIC outlet.
Electrical outlets in the cabinet are restricted to 5 amps
Do not use any detachable power cord that is not adequately
Chapter 2: Prerequisites
maximum current.
Prises électriques dans l'armoire sont limitées à 5 courant
maximum ampères.
rated for the unit.
Ne pas utliser un fil électrique amovible qui n’est pas du tension
nominale de l’appareil.
Service Line Requirements
All utility service lines should be ¼ inch O.D., brass, copper, or stainless
steel, and equipped with an easily accessible shut-off valve. If the service line
pressure exceeds 40 PSI, it must be equipped with a pressure regulator to
reduce the line pressure.
Note: The use of flammable gases or solvents should be avoided in the
biosafety cabinet. Open flame in the cabinet will disrupt the laminar airflow
in the cabinet and may damage the HEPA filters. Flammable gases or
solvents may reach explosive concentrations in the cabinet or ductwork. If
you feel that the procedure requires the use of an open flame or flammable
materials, contact the institution’s safety office.
The use of air or gases under high pressure should be avoided as they may
seriously disrupt the airflow patterns in the cabinet.
Product Service 1-800-522-7658
5
Original instructions
Chapter 2: Prerequisites
User-Supplied Microscope Requirements –
Purifier Cell Logic Biosafety Cabinets with
Scope-Ready™ Package Only
If installing a microscope, locate the Stand-Still™ Isolation Platform in a
separate box shipped with the cabinet. The Stand-Still Isolation Platform
supports the microscope on top of the stainless steel work surface to
eliminate up to 94% of vibrations and to promote safe airflow. If using a
stereoscope with a forward extending base, the extended Stand-Still XL
Isolation Platform should be ordered from Appendix D: Accessories. Figures
from Appendix F should be used to assist your set-up.
On models with the Scope-Ready package, the Cell Logic Biosafety Cabinet
includes a clear, disposable Pure-Vu™ Seal that can be altered to
accommodate most microscope’s eyepieces. Utilize the Punch Kit
(#4027801), supplied with your cabinet, to cut the two eyepiece holes at
approximately 2.75" (69.9 mm) spacing to match the height of your scope.
As a convenience, pre-cut Pure-Vu eyepiece seals can be ordered from
Appendix D: Accessories. The pre-cut accessory Pure-Vu Seals are based off
the nominal height from the base of the microscope to eyepiece centerline
and can save you valuable time modifying the seal shipped with the Cell
Logic. If using a stereoscope with a forward extending base, one of the PureVu XL Seals should be ordered from Appendix D: Accessories. Figures from
Appendix F should be used to assist your set-up for all Pure-Vu Seals.
User Supplied Circulator Requirements – Purifier
Cell Logic Biosafety Cabinets with Temp Zone™
Package Only
The Temp Zone™ features a temperature controlled area on the work surface
where heated or chilled samples may be transferred and kept at optimum
temperature. A user-supplied circulator that continually delivers heated or
chilled water at a minimum flow rate of 0.5 GPM (1.9 LPM) with typical
volume of 6 liters is required and may be obtained from your laboratory
supply dealer. See Figure 3-6 in Chapter 3.
Quick connection fittings are included on the left side of the cabinet and are
sized for 3/8" diameter polyethylene tubing. As a convenience, an ample
supply (20'; 6 m) of 3/8" (9.5 mm) diameter tubing, insulation, and quick
connect fittings (3/8 tube x 1/8 NPT, 3/8 tube x 1/4 NPT, 3/8 tube x 3/8 NPT)
are supplied with the Temp-Zone package for connection to a water
circulator. The water circulator can be located underneath the work surface
of the cabinet or next to the cabinet on the floor.
6
Product Service 1-800-522-7658
Original instructions
CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Now that the installation is properly prepared, you are ready to inspect,
install, and certify the Logic+ biosafety cabinet. This chapter covers how to:
• Unpack and move the biosafety cabinet.
• Install the cabinet.
• Connect the electrical supply source.
• Connect the service lines.
• Connect to an exhaust system (optional).
• Arrange certification of the biosafety cabinet.
• Install the microscope (optional – Scope-Ready package only).
• Connect the circulator (optional – Temp-Zone package only).
Tools required for installation the biosafety cabinet include two 1/2"
wrenches, a flat-blade screwdriver, a #2 Phillips screwdriver, and a
carpenter’s level.
Note: The biosafety cabinet models weigh between 400–700 lbs. (182-318
kg). The shipping pallet allows for lifting with a mechanical lift truck or
floor jack. If you must lift the product manually, use at least six (6) persons
and follow safe-lifting guidelines.
Product Service 1-800-522-7658
7
Original instructions
Chapter 3: Getting Started
Unpacking the Biosafety Cabinet
Carefully remove the outer carton and inspect the cabinet for damage that
may have occurred in transit. If the biosafety cabinet is damaged, notify the
delivery carrier immediately and retain the entire shipment intact for
inspection by the carrier.
Note: United States Interstate Commerce Commission rules require that
claims be filed with the delivery carrier within fifteen (15) days of delivery.
Do not return goods without the prior authorization of Labconco.
Unauthorized returns will not be accepted.
If the cabinet was damaged in transit, you must file a claim directly with the
freight carrier. Labconco Corporation and its dealers are not responsible for
shipping damages.
Do not discard the carton or packing material for the biosafety cabinet until
all of the components have been checked, installed and tested.
The cabinet is secured to the pallet in two places on each side. To access the
nuts and bolts, remove the side panels by removing and keeping the two
Phillips screws on both panels. Swing the front of each panel away from the
cabinet, and lift it straight up to remove the panel from the cabinet.
Note: The side panels must be removed to access the fasteners that secure the
biosafety cabinet to the pallet. DO NOT ATTEMPT TO LIFT THE
BIOSAFETY CABINET BY THE SIDE PANELS; DAMAGE WILL
OCCUR.
Preparing the Biosafety Cabinet for Operation
Installation instructions (Labconco P/N 1056801) are attached to the sash of
the biosafety cabinet. If these instructions are missing or unclear, contact
Product Service at 800-821-5525 or 816-333-8811. The following are located
in a box underneath the work surface:
• User’s Manual CD
• Drain Valve Assembly and fasteners
• Power Cord
• Product Registration Card
• Vacu-Pass
If you did not receive one or more of the components listed for the cabinet, or
if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.
8
TM
Accessories (optional)
Product Service 1-800-522-7658
Original instructions
Moving and Lifting the Cabinet
Move the cabinet, attached to its pallet, by using a floor jack, or a furniture
dolly underneath the unit. DO NOT move the cabinet by tilting it onto a hand
truck.
When lifting the cabinet DO NOT lift the cabinet in the middle front area of
the hull. Lifting here may bend or distort the bottom of the cabinet, causing
damage to the unit.
Chapter 3: Getting Started
Installing the Biosafety Cabinet on an Existing
Work Surface
Note: The biosafety cabinet is very top heavy. Use caution when lifting or
moving it.
When installing the biosafety cabinet onto an existing work surface or benchtop,
ensure that the structure can safely support the combined weight of the cabinet
and any related equipment. The work surface should be at least as wide as the
cabinet and 31 inches (787 mm) deep to properly support the unit.
A hole or notch may be cut in the supporting surface in the right front corner
to accommodate the optional drain valve.
Installing the Cabinet on a Labconco Base Stand
Labconco offers accessory Base Stands in a variety of configurations to suit
your particular needs. Stands can be ordered with adjustable telescoping legs
or with a manually or electrically adjustable hydraulic lift.
Product Service 1-800-522-7658
9
Original instructions
Chapter 3: Getting Started
Telescoping Base Stands
These stands are included with some Logic+ models, or are available
separately. The base stands for each width cabinet are listed in Table 3-1
below.
Width Base Stand w/Feet Model #
4' 3401004
6' 3401006
Table 3-1
Manual or Electric Hydraulic Lift Base Stands
These base stands offer infinitely adjustable height between 25.5 and 33.5
inches (648 to 851 mm), giving a cabinet work surface height of 28.0 to 36.0
inches. The height is adjusted either by a manual (hand crank) or electric
pump that drives hydraulic rams in the legs of the stands. All of the hydraulic
stands are equipped with fixed feet, but can be converted to caster wheels
with the addition of Caster Kit #3784000. The base stands for each cabinet
size is listed in Table 3-2 below.
Width Manual Lift Stand # Electric (115V) Lift Stand # Electric (230V) Lift Stand #
4' 3780201 3780101 3780104
6' 3780202 3780102 3780105
Table 3-2
Note: When installing the cabinet on a hydraulic lift base stand, a local codecompliant flexible duct connection must be used between the Logic+’s
exhaust connection and the exhaust ductwork. Ensure that the hydraulic lines
and the electrical cord are clear of any obstructions before installing the
cabinet on the stand or operating the lift system.
10
Product Service 1-800-522-7658
Original instructions
Connecting the Biosafety Cabinet to Utility
Service Lines
Note: Some models have a solenoid valve connected to the service valve on
the right side, rear position. The solenoid prevents gas from flowing to the
service valve when the unit blower is off. It is the only service valve position
that can be fitted with a solenoid valve. Connect the gas service to the
solenoid valve.
The service lines (if any) should be connected to the tube fitting(s) on the
outside of the liner wall as shown in Figure 3-1. To install the tubing, follow
these steps:
1. Ensure that the tubing is ¼ inch O.D., soft metal, and that the end has
been completely deburred.
2. Route the tubing from the rear of the cabinet, ensuring that it will line up
with the slot in the back of the side panel. The slot is located from 8 ¾ to
11 ¼ inches (222 to 288 mm) from the bottom of the cabinet.
Chapter 3: Getting Started
Note: Make sure that the tube routing will not contact any electrical wires.
DO NOT loop service line tubing within the side panels of the cabinet.
3. Make sure that the nut on the tube fitting is loose, but do not remove it.
Look inside the fitting to make sure the tube ferrule is there.
4. Push the tube into the fitting until it is properly seated. The tube will go
approximately ¾ inch (19 mm) into the fitting.
5. Tighten the tube fitting nut hand tight and then, using a 7/16-inch wrench,
tighten it at least ¾ turn more.
6. Close the service valve in the biosafety cabinet and then slowly open the
shutoff valve on the service valve. Test all fittings for leakage. Tighten
the tube nut slightly if needed.
Product Service 1-800-522-7658
Figure 3-1
Tube fitting nut
Tube fitting
11
Original instructions
Chapter 3: Getting Started
Optional Vacu-PassTM Cord & Cable Portal Use
Note: There must be enough clearance to pass the cord or cable between the
Logic+’s exterior dress panel and any obstruction.
Note: Some Vacu-Pass components and the cord or cable passing through it
may become contaminated during use of the cabinet. Ensure all potentially
contaminated components are surface decontaminated before handling or
removal from the cabinet.
1. Remove the grommet from the liner side wall. Remove the solid sealing
plug from the body of the portal by either pressing it through from the
outside, or by carefully inserting a spatula or similar device between the
sealing plug and the body of the portal, and prying the plug out.
2. Pass the cord or cable through the body of the portal, and then through
one of the plugs that has been cut for cord or cable use, then through the
grommet, as shown in Figure 3-2.
Note: select a plug with a hole that is slightly smaller than the cord or cable,
to create a proper seal. This will also help minimize movement of the cord or
cable if it is accidentally pulled during use.
Figure 3-2
3. Position the cord or cable as it will be used in the cabinet, and then push
the plug back into the body of the portal until it seats in the portal.
Reinstall the grommet.
12
Product Service 1-800-522-7658
Original instructions
Drain Valve Installation
In order to prevent damage during shipping, the drain valve assembly has not
been installed. If desired, the valve should be installed after the cabinet is in
its final location.
To install the valve assembly, follow these steps:
Note: The work surface is heavy. Use caution when handling it.
1. Lift the work surface out of the biosafety cabinet by lifting on the knobs
at the front of the work surface. Steady the work surface while pulling it
straight out the front of the cabinet.
2. Using a putty knife, remove and discard the stainless steel cover that is
sealed over the drain mounting holes. Scrape out remaining sealant that is
around the holes.
3. Apply a light coating of silicone sealant (user supplied) to the mounting
surface of the drain assembly. Attach the drain assembly under the
bottom of the cabinet as shown in Figure 3-3. Wipe off any excess sealant
from the cabinet bottom. Ensure that the center drain hole is unobstructed.
Chapter 3: Getting Started
4. Make sure the drain valve is in the closed position.
5. Reinstall the work surface.
6. Allow the silicone sealant to cure for at least eight hours before exposing
it to liquid.
NOTE: The drain valve assembly
attaches to the underside of the cabinet
bottom.
Apply a light coat of silicone sealant to
this surface of the connector, aligning the
three holes in the connector with the three
holes in the biosafety cabinet liner.
Figure 3-3
Product Service 1-800-522-7658
13
Original instructions
Chapter 3: Getting Started
Microscope Installation (optional)
In order to successfully install a user-supplied microscope in a Cell Logic
Biosafety Cabinet with Scope-Ready package, please follow the steps below
and refer to Figure 3-4 as well as Appendix F: Installation Guide for all
Pure-Vu Seals and Stand-Still Isolation Platforms:
Note: The work surface is heavy. Use caution when handling it.
1. Before installing a microscope, locate the Stand-Still Isolation
Platform that is packaged in a separate carton underneath the work
surface. Note: Stereoscopes will typically require the Stand-Still XL
Isolation Platform found in www.labconco.com.
2. Install the Stand Still Isolation Platform so that the leading edge is in
line with the work surface leading edge. The Stand-Still Isolation
Platform is elevated to promote safe airflow and isolate up to 94% of
vibrations. See Appendix F for typical pictures of proper set-up of the
Stand-Still Isolation Platform.
3. With the sash in the up position, place a user-supplied microscope
inside and on top of the Stand-Still Isolation Platform. Remove the
microscope eyepieces.
4. The Cell Logic Biosafety Cabinet with Scope-Ready Package
includes one Pure-Vu Seal that requires 0.5" (12.7 mm) diameter
holes spaced approximately 2.75" (69.9 mm) apart and located from
the base of the microscope to the centerline of the eyepiece. The
eyepiece holes can be cut with the 0.5" (12.7 mm) diameter hole
Punch Kit (#4027801) supplied with your cabinet.
For your convenience, pre-cut accessory Pure-Vu Seals are available per
www.labconco.com
5. If punching the 0.5" (12.7 mm) diameter holes, remove the
microscope eyepieces and line up the centerline of the eyepiece base
with the seal. Then mark the centerline of each eyepiece hole spaced
at approximately 2.75" (69.9 mm).
6. Remove the 14 screws (#6-32 x 0.25") that retain the Pure-Vu Seal.
7. Either reinstall one of the pre-cut accessory Pure-Vu Seals from
www.labconco.com
then re-install the seal. For your convenience, see Appendix F for
installation of pleated Pure-Vu XL Seals used with stereoscopes
requiring frequent eyepiece height adjustment of +/- 2.0" (51 mm).
8. After the seal is re-installed, then move the microscope forward to
carefully stretch the Pure-Vu Seal over both of the microscope
eyepiece bases.
9. Finally, re-install the microscope eyepieces.
, and may be an easier installation.
or punch the holes in the blank seal supplied and
14
Product Service 1-800-522-7658
Original instructions
10. The Pure-Vu Seal should now provide an optimum seal for safe
Chapter 3: Getting Started
airflow and ergonomic visibility.
Figure 3-4
Outer
Aerodynamic
Collar
Pure-Vu Seal
Mounting
Screws
Product Service 1-800-522-7658
15
Original instructions
Chapter 3: Getting Started
Circulator Installation (optional)
In order to successfully install a user-supplied circulator on a Cell Logic
Biosafety Cabinet with Temp-Zone package please follow the steps below
and refer to Figure 3-5:
Note: The work surface is heavy and two Easy-Clean™ Prop Rods are
provided to pivot and support the Temp-Zone work area and the work
surface.
1. The Temp-Zone work area has supply and return lines for the user-
supplied circulator. Refer to Figure 3-5 and locate the two quick
connect elbows on the left side of the cabinet behind the left side
panel.
2. Find a suitable place next to the cabinet or underneath the cabinet to
place the circulator. Accessory shelves for small circulators are
available from www.labconco.com. Larger circulators should be
placed on the floor.
3. Pivot the work surface up and use the Easy-Clean Prop Rods to
support the work surface. Then locate the tubing, insulation, and
quick connect fittings shipped with your Cell Logic. The tubing is
3/8" OD (9.5 mm) and tube inserts must be placed inside the tubing to
properly support the tubing inside the quick connect fitting. 20 feet
(6.1 meters) of tubing length is provided for your convenience.
Note: The tube inserts prevent the tubing from collapsing at
connection points and must be used.
4. Plan, route and cut the tubing from the two elbows on the left side of
the cabinet to the circulator.
5. Locate the quick connect fittings (3/8" tube x 1/8 NPT, 3/8" tube x 1/4
NPT, and 3/8" tube x 3/8 NPT) and install the appropriate fittings to the
user-supplied circulator with appropriate pipe sealant or PTFE tape.
6. Place tube inserts in all ends of the cut-to-size tubing.
7. Cut and install the insulation around the cut tubing lengths.
8. Insert cut tubing lengths into the elbows on the cabinet and then
install both tubing lengths into the circulator to complete the water
circulator loop.
9. Read and follow the circulator instructions before operating. The
circulator should have a minimum flow rate of 0.5 GPM (1.9 LPM).
In all cases, the temperature of the circulator will not equal the
temperature of samples on the Temp-Zone. Factors such as the
sample vessels, sample sizes, aluminum tube racks, and length of
tubing connecting the circulator to the Temp-Zone can influence the
sample temperature. Circulator settings to hold heated incubator
sample temperatures may require 0.3-2.0°C higher temperatures than
the desired sample temperature. Furthermore, circulator settings to
maintain chilled refrigerated samples may require 0.3-3.0°C colder
temperatures than the desired sample temperature. Experimentation is
necessary to achieve proper sample temperatures.
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Product Service 1-800-522-7658
Original instructions
10. For chilled applications, the Temp-Zone work area comes with a drain
Chapter 3: Getting Started
line installed that can be routed to the cabinet’s drain valve, if desired.
This Temp-Zone drain line installation is optional for chilled
applications should excessive condensation occur under the work
surface. Simply snap in the supplied press-fit elbow to the drain valve
shown in Figure 3-5. Follow the diagram to finish the installation.
Figure 3-5
Quick-Connect
Elbow Fitting
Circulator
(User-Supplied)
Circulator Tubing
and Insulation
Prop Rod
Press-fit
Elbow
Drain Valve
Optional
Drain Line
Temp-Zone
Product Service 1-800-522-7658
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