Labconco Purifier Filtered PCR Enclosures User Manual

Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com

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User’s Manual

Models

3970202, 3970205, 3970222, 3970225, 3970302, 3970305, 3970322, 3970325, 3970402, 3970405, 3970422, 3970425

Please read the User’s Manual before operating the equipment.

Purifier® Filtered PCR Enclosures

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of defective material and workmanship, provided such defect results from normal and proper use of the equipment. Glassware is not warranted from breakage when dropped or mishandled.

The warranty for Purifier® Filtered PCR Enclosures will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to the owner (organization) of record.

This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This statement may be altered by a specific published amendment. No individual has authorization to alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or accidental breakage is not covered.

The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with

any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,

land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.

Part #3938201, Rev. C

ECO J457

ORIGINAL INSTRUCTIONS

TABLE OF CONTENTS

CHAPTER 1: INTRODUCTION 1

ISO Class 5 Definition 2 Polymerase Chain Reaction Definition 2 About This Manual 3 Typographical Conventions 4

CHAPTER 2: PREREQUISITES 5

Support, Vibration & Movement Requirements 5 Location and Air Current Requirements 6 Airflow Specifications 6 Electrical Requirements 7 Space Requirements 7

CHAPTER 3: GETTING STARTED 8

Unpacking Your Enclosure 9 Installing the Filtered PCR Enclosure on a Supporting Structure

and Work Surface 9

Verifying HEPA Filter Integrity 11

Connecting the Electrical Supply Source to the Filtered PCR

Enclosures 11 Setting the Downflow Velocity with Speed Control 12 Validating the Filtered PCR Enclosure 12 Sealing the Filtered PCR Enclosure to the Work Surface 13

CHAPTER 4: PERFORMANCE FEATURES AND

SAFETY PRECAUTIONS 14

Components 15 Safety Precautions 19

CHAPTER 5: APPROPRIATE APPLICATIONS FOR YOUR

FILTERED PCR ENCLOSURE 21

Routine Daily Work Procedures 21 Suitable Applications 22 HEPA Filter Applications, Suitability & Guidelines 22

CHAPTER 6: MAINTAINING YOUR FILTERED PCR

ENCLOSURE 24

Routine Maintenance Schedule 25 When to Replace HEPA Filters 25 How to Install a New HEPA Filter 26

HEPA Filter Leak Test 28 Setting the Downflow Velocity with the Speed Control Adjustment 29 Calibrate and Operate the Airflow Monitor 29 Initial Certification 33 Re-Certification 33 Fluorescent Light Replacement 33 UV Light Replacement 33 Motorized Impeller Replacement 34 Speed Control Replacement 36

CHAPTER 7: ACCESSORIZING & MODIFYING YOUR

FILTERED PCR ENCLOSURE 37

CHAPTER 8: TROUBLESHOOTING & SERVICER OPERATING LOG 40

APPENDIX A: FILTERED PCR ENCLOSURE COMPONENTS AND REPLACEMENT PARTS 44

APPENDIX B: DIMENSIONS 49

APPENDIX C: FILTERED PCR ENCLOSURE

SPECIFICATIONS 50

APPENDIX D: REFERENCES 51

CHAPTER 1

INTRODUCTION

Congratulations on your purchase of a Labconco Purifier® Filtered PCR Enclosure. Your enclosure provides a particulate free environment for Polymerase Chain Reaction experiments. It is the result of Labconco’s more than 30 years experience in manufacturing filtered enclosures.

These enclosures will effectively provide product protection from airborne particulate matter. During operation, room air is drawn through the prefilter on top, forced through the blower and then through the HEPA filter and diffuser. The clean air in the work area in the enclosure meets or exceeds ISO Standard Class 5 conditions defined per ISO 14644-1 and Class 100 conditions as defined by Federal Standard 209E. The enclosure is set to deliver nominal downward airflow of 40-55 feet per minute. Each enclosure uses a single HEPA filter, which is rated at least 99.99% efficient for 0.3-micron particles. The Purifier Filtered PCR enclosures offer many unique features to enhance performance. A variable solidstate digital timer provides a UV light to destroy contaminating DNA and RNA in the work area. The UV light automatically turns off to prepare for the next experiment. To take full advantage of all features, please acquaint yourself with this manual and keep it handy for future reference. If you are unfamiliar with how Filtered PCR Enclosures operate, please review Chapter 4: Performance Features and Safety Precautions before you begin working. Even if you are an experienced user, please review Chapter 5: Using Your Filtered PCR Enclosure, which describes the enclosure features.

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Chapter 1: Introduction

Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.

ISO

classification

number (N)

Maximum concentration limits (particles/m3 of air) for particles equal to and larger than the considered sizes shown below (concentration limits are calculated in accordance with 3.2 of Standard 14644-1)

0.1 µm

0.2 µm

0.3 µm

0.5 µm

1 µm

5 µm

ISO Class 1

10 2

ISO Class 2

100

10 4

ISO Class 3

1 000

237

102

35 8

ISO Class 4

10 000

2 370

1 020

352

ISO Class 5

100 000

23 700

10 200

3 520

832

ISO Class 6

1 000 000

237 000

102 000

35 200

8 320

293

ISO Class 7 352 000

83 200

2 930

ISO Class 8

3 520 000

832 000

29 300

ISO Class 9

35 200 000

8 320 000

293 000

ISO Class 5 Definition

Airborne particulate cleanliness inside any PCR Enclosure is designated by ISO Class 5, which is equivalent to 3520 particles 0.5 µm or larger per cubic meter of air per ISO Standard 14644-1. ISO Class 5 cleanliness is illustrated in the table to follow and is equivalent to Class 100 air conditions as defined by Federal Standard 209E. Class 100 is equal to 100 particles 0.5 µm or larger per cubic foot of air.

Table 1-1 ISO Classification Number (N)

Polymerase Chain Reaction (PCR) Definition

Polymerase Chain Reaction (PCR), is a laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated, producing a large, readily analyzed sample of a piece of DNA. In PCR, DNA is immersed in a solution containing the enzyme DNA polymerase, unattached nucleotide bases (the

subunits that DNA is composed of), and “primers”, short sequences of nucleotides

designed to bind with an end of the desired DNA segment. Two primers are used: one primer binds at one end of the desired segment on one of the two paired DNA strands and the other primer binds at the other end but on the other strand. The solution is heated to break the bonds between the strands of the DNA. When the solution cools, the primers bind to the separated strands, and DNA polymerase quickly builds a new strand by joining the free nucleotide bases to the primers. When this process is repeated, a strand that was formed with one primer binds to the other primer, resulting in a new strand that is restricted solely to the desired segment. Thus, the region of DNA between the primers is selectively replicated. Further repetitions of the process can produce billions of copies of a small piece of DNA in several hours. PCR was developed in 1985 by Kary B. Mullis, who was awarded the 1993 Nobel Prize in chemistry for his work. It is used in a broad range of applications from DNA fingerprinting to medical tests to identify diseases

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Chapter 1: Introduction

About This Manual

This manual is designed to help you learn how to install, use, and maintain your Filtered (PCR) Enclosure.

If the unit is not operated as specified in this manual it may impair the protection provided by the unit.

Si l'unité n'est pas utilisée comme spécifié dans ce manuel il peut diminuer la protection fournie par l'unité.

Chapter 1: Introduction provides a brief overview of the Filtered PCR Enclosure, explains the organization of the manual, and defines the typographical conventions used in the manual.

Chapter 2: Prerequisites explains what you need to do to prepare your site before you install the Filtered PCR Enclosure. Electrical and service requirements are discussed.

Chapter 3: Getting Started contains the information you need to properly unpack, inspect, install, and certify the Filtered PCR Enclosure.

Chapter 4: Performance Features and Safety Precautions explains how the Purifier Filtered PCR Enclosure operates and the appropriate precautions you should take when using it.

Chapter 5: Using Your Filtered Enclosure discusses the basic operation of how to prepare, use and shut down your Filtered PCR Enclosure.

Chapter 6: Maintaining Your Filtered PCR Enclosure explains how to perform routine maintenance on the PCR Enclosure.

Chapter 7: Accessorizing Your Filtered PCR Enclosure explains acceptable modifications to the PCR Enclosure or how to add accessories.

Chapter 8: Troubleshooting contains a table of problems you may encounter while using the Filtered PCR Enclosure including probable causes and suggested corrective actions.

Appendix A: Components and Replacement Parts contains labeled diagrams of all of the components of the Filtered PCR Enclosure.

Appendix B: Dimensions contains comprehensive diagrams showing all of the dimensions for the Filtered PCR Enclosure.

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Chapter 1: Introduction



Appendix C: Filtered PCR Enclosure Specifications contains the electrical requirements for Filtered PCR Enclosures. Wiring diagrams are also included.

Appendix D: References lists the various resources available addressing clean work stations and PCR Enclosures.

Typographical Conventions

Recognizing the following typographical conventions will help you understand and use this manual:

 Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:

Getting Started).

 Steps required to perform a task are presented in a numbered format.  Comments located in the margins provide suggestions, reminders, and

references.

 Critical information is presented in boldface type in paragraphs that are

preceded by the exclamation icon. Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to the enclosure.

 Critical information is presented in boldface type in paragraphs that are

preceded by the wrench icon. Only a trained certifier or contractor should only perform these operations. Failure to comply with the information following a wrench icon may result in injury to the user or permanent damage to your PCR Enclosure.

 Important information is presented in capitalized type in paragraphs that are

preceded by the pointer icon. It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user.

 A number icon precedes information that is specific to a particular model of

enclosure. The 2' icon indicates the text is specific to the 2-foot wide model. The 3' icon indicates the text is specific to the 3-foot model, etc.

 CAUTION – See Manual. When this symbol is on the unit it indicates a

caution that is detailed in this manual.

 ATTENTION - Voir manuel. Lorsque ce symbole est sur l'unité, il indique une

mise en garde qui est indiqué dans ce manuel.

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CHAPTER 2 PREREQUISITES

Before you install the Filtered PCR Enclosure, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the Filtered PCR Enclosure, and other apparatus. Additionally, the enclosure should be strategically placed in the lab to provide efficient workflow.

Carefully read this chapter to learn the requirements for your installation site:

 The support, vibration and movement requirements.  The location and air current requirements.  The exhaust and blower requirements.  The electrical power requirements.  The space requirements.

Refer to Appendix B: Dimensions for complete enclosure dimensions.

Refer to Appendix C: Filtered PCR Enclosure Specifications for complete filtered enclosure electrical and environmental conditions, specifications and requirements.

Support, Vibration and Movement Requirements

At a minimum, the supporting structure usually consists of a base cabinet or base stand and chemically-resistant work surface.

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Chapter 2: Prerequisites

Enclosure

Width

Model Description

Downflow

Velocity

(fpm)

Out-

Flow

Exhaust

Volume

(CFM)

Noise

Pressure

db(A)

Average

Outflow

Face

Velocity

(fpm)

2' Filtered PCR Enclosure

30 40 50 60

120 165 210 250

53-57 58-60 60-61 62-63

85 115 145 170

3' Filtered PCR Enclosure

30 40 50 60

180 250 310 370

58-60 60-62 62-66 66-70

85 115 145 170

4' Filtered PCR Enclosure

30 40 50 60

240 330 420 500

58-62 60-64 62-66 66-70

85 115 145 170

Location and Air Current Requirements

The Purifier Filtered PCR Enclosures have been designed to provide particulate free air meeting ISO Class 5 conditions by negating typical cross drafts and turbulence within the opening. However, as a precautionary safety measure and a higher level of quality management, it is recommended that the Filtered PCR Enclosure be placed in an area away from:

 High traffic areas where walking might cause an air disturbance or be a

nuisance.

 Overhead or wall HVAC diffusers, fans, radiators or other lab equipment

producing air currents.

 Doorways or windows that may be opened.

Do not position the unit so that it is difficult to operate the main disconnect device.

Ne placez pas l'appareil de sorte qu'il est difficile de faire fonctionner le dispositif principal de déconnexion.

Airflow Specifications

The Purifier Filtered PCR Enclosures use an integral motorized impeller to draw room air from the top and through the enclosure. This room air is then pushed down through the HEPA filter. The enclosure is factory set to deliver 40-55 fpm downflow velocity measured 6" below the upper diffuser. The Class 5 HEPAfiltered air flows across the work area and exits out the front and back of the enclosure. The Filtered PCR Enclosure provides a continuous downflow of particulate free HEPA filtered air.

Data for the outflow exhaust volume, downflow velocities and out-flow face velocities are listed for each Filtered PCR model below.

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Chapter 2: Prerequisites

Electrical Requirements

Standard duplex electrical receptacles should be nearby for connecting the Filtered PCR Enclosure, or other equipment. The enclosure includes iris pass-throughs to allow electrical cords through the back of the enclosure without leaving a large hole.

Space Requirements

The dimensions for all the different models are shown in Appendix B: Dimensions.

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CHAPTER 3

GETTING STARTED

Now that the site for your Filtered PCR Enclosure is properly prepared, you are ready to unpack, inspect, install, and validate your enclosure. Read this chapter to learn how to:

 Unpack and move the enclosure.  Set up the enclosure with the proper supporting structure and work surface.  Verify HEPA filters integrity.  Connect the electrical supply.  Set the downflow velocity with the speed control adjustment.  Validate enclosure airflow.  Seal the enclosure to the work surface.

Depending upon which model you are installing, you may need common mechanical and electrical installation tools in addition to wrenches, ratchets, sockets, a nut driver set, a flat-blade screwdriver, a Phillips screwdriver, and a carpenter level to complete the instructions in the chapter.

Each enclosure model weighs between 125 to 195 lbs. each (55 to 85 kg). The shipping container allows for lifting with a mechanical lift truck or floor jack. If you must lift the

enclosure manually, follow safe-lifting guidelines. Do not lift

by the lower air foil.

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Chapter 3: Getting Started



The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Unpacking the Enclosure

Carefully remove the shrink-wrap or carton on the enclosure and inspect it for damage that may have occurred in transit. If damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.

DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.

IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.

Do not discard the packing material until you have checked all of the components and tested the enclosure. We recommend that you do not remove the enclosure from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack.

Do not move the enclosure by tilting it onto a hand truck.

Installing the Filtered PCR Enclosure on a Supporting Structure and Work Surface

Use caution when lifting or moving the enclosure.

When installing the enclosure onto a chemical-resistant work surface or benchtop, ensure that the structure can safely support the combined weight of the enclosure and any related equipment. The work surface should be at least as wide and deep as the enclosure to properly support it. The front of the enclosure should be aligned within 0.30" (8mm) of the front of the work surface. Mounting holes are provided in the Labconco accessory work surfaces to secure the enclosure.

Work Surface Specifications

The work surface should be smooth, rigid and durable, such as a chemical-resistant epoxy resin. The surface should be non-porous and resistant to the materials used in conjunction with the Purifier Filtered PCR Enclosure. The work surface should also contain a dished recessed area for containing primary spills.

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Chapter 3: Getting Started

Filtered PCR Enclosure Work Surface Installation

1. Level the base cabinet or stand and the work surface. See Figure 3-1.

2. Position the work surface in its intended location and with the front of the

work surface towards you. (Rear mounting holes are located close to the rear edge.)

3. Secure the work surface to the base cabinet or stand with a structural

adhesive or silicone sealant.

4. Insert the supplied mounting screws in the four holes. Allow a minimum

of 1/8" clearance under the head of the screw for positioning the enclosure.

5. Place the enclosure on the work surface and slide the rear flange and front

air foil flanges under the mounting screw heads.

6. Tighten the four screws to complete the installation.

Figure 3-1

Filtered PCR Enclosure Installation

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Chapter 3: Getting Started

HEPA Filter

Appropriate Use

Filter Size

Part No.

HEPA filters are high-efficiency particulate air filters having a particulate removable efficiency of 99.99% for particles with a diameter of 0.3 micron.

3707900

3707901

3707902

Verifying HEPA Filter Integrity

The HEPA filter is shipped installed with the gasket on the downstream side. The HEPA filter is leak checked at Labconco. A second leak check is recommended before using the enclosure and at least annually thereafter. Consult your Safety Officer and Chapter 6 for the HEPA Filter Leak Test. See Figure 4-2 for HEPA filter location, HEPA filter gasket, and filter clamp bolts. All seams downstream of the HEPA filter are jacketed by positive pressure. This intrinsically safe design ensures Class 5 conditions and a particulate free environment for PCR experiments.

Connecting the Electrical Supply Source to the Filtered PCR Enclosure

115V Models

Simply connect the 115V power cord supplied to the IEC electrical supply plug on the back of the enclosure. If using at 50 Hz operations, blower performance maximum airflow will be reduced by 17%.

230V Models

The 230V is shipped without a plug. Install the appropriate plug for your electrical specifications per local codes.

Do not use any detachable power cord that is not adequately rated

for the unit.

Ne pas utliser un fil électrique amovible qui n’est pas du tension nominale de l’appareil.

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Chapter 3: Getting Started

Setting the Downflow Velocity with the Speed Control

Adjustment of the speed control gives the correct downflow velocity and is located behind the front panel. The downflow velocity should be from 30-65 fpm for Class 100 (ISO Class 5) conditions. ISO Class 5 clean air conditions are maximized at a setting within this range. The Filtered PCR Enclosure is factory set at 40-55 fpm downflow velocity. (Consult your Safety Officer for airflow recommendations for your application). Working at the lowest downflow velocity appropriate for the application will give the quietest operation. Downflow velocity measurements are made using an anemometer. An electric anemometer can be obtained from your laboratory supply dealer. Downflow velocity measurements should be taken 6" below the interior upper diffuser. Using a small Phillips screwdriver, adjust the speed control to give the required downflow velocity. See Figures 6-1 and 6-2 in Chapter 6 to locate the speed control. The downflow velocity is increased by turning the speed control counterclockwise and clockwise to decrease downflow velocity.

Validating the Filtered PCR Enclosure

To determine the actual downflow velocity, airflow velocity readings are taken. This should be done 6" down from the upper diffuser located inside the top of the

enclosure. The “average downflow velocity” is achieved by averaging the

readings in the downflow test grid for each model: The downflow test grid consists of 9 test points, 3 points in 3 rows. The back row

of the grid is 6" from the rear of the work area. The middle and front rows are

6.57" apart from the back to the front. The first and last column of the downflow grid are spaced 6" in from each sidewall with a center column spaced 5.32" away from the first and last column.

The downflow test grid consists of 12 test points, 4 points in 3 rows. The back row of the 6" from the rear of the work area. The middle and front rows are 6.57" apart from the back to the front. The first and last column of the downflow grid are spaced 6" in from each sidewall with two columns spaced 7.54" away from the first and last column.

The downflow test grid consists of 15 test points, 5 points in 3 rows. The back row of the grid is 6" from the rear of the work area. The middle and front rows are

6.57" apart from the back to the front. The first and last column of the downflow grid are spaced 6" in from each sidewall with three columns spaced 8.66" away from the first and last column.

Refer to table in Chapter 2 Refer to the exhaust specification for proper airflow volumes and downflow velocities for your particular model. A certified technician should complete validation.

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Chapter 3: Getting Started

The Purifier Filtered PCR Enclosures have been tested at Labconco before shipment and provide ISO Class 5 clean air conditions inside the enclosure. Labconco also performed extensive performance testing to validate the Filtered

PCR’s for product protection under ISO Class 5 conditions. For copies of these

validation reports, contact Labconco Customer Service.

Sealing the Filtered PCR Enclosure to the Work Surface

When the Filtered PCR Enclosure has been set in place and wired, it may be sealed at the work surface to prevent materials from collecting under the walls. A bead of silicone sealant is recommended to seal the Filtered PCR Enclosure to the work surface.

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CHAPTER 4

PERFORMANCE FEATURES AND SAFETY PRECAUTIONS

The Purifier Filtered PCR Enclosure is designed to provide particulate free protection from airborne matter. During operation, room air is drawn from the top and filtered by the HEPA filter providing Class 5 conditions inside the enclosure. The Purifier Filtered PCR Enclosure delivers Class 5 air conditions when operating at downflow velocities of 30 to 65 feet per minute. The HEPA filter is jacketed by positive pressure preventing contaminated air inside the enclosure and ensuring Class 5 air conditions inside the enclosure. After each Polymerase Chain Reaction (PCR) experiment, the variable timed UV light can be run to decontaminate the work area from DNA and RNA.

The Purifier Filtered PCR Enclosure HEPA filter is located behind the front panel. Users are encouraged to routinely check airflow to satisfy Class 5 air conditions inside the enclosure or purchase a model with an airflow monitor. For additional surface decontamination inside the enclosure, users are encouraged to use the variable digital timed UV light and UV sash closure.

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Located

Behind

4 2 12

Control Panel

Chapter 4: Performance Features and Safety Precautions

Located

Behind

Figure 4-1

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Chapter 4: Performance Features and Safety Precautions

Airflow

Switch with Adjustment

Screw

UV Light

(Not

Shown)

16, (Not

Shown)

Figure 4-2

Filtered PCR Enclosure Airflow Diagram

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Chapter 4: Performance Features and Safety Precautions

Figure 4-3

UV Sash Closure Panel

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Chapter 4: Performance Features and Safety Precautions

1. Aerodynamic Lower Air Foil allows air to sweep the work surface and

promote ISO Class 5 air conditions inside the enclosure. See Figure 4-1.

2. Ergonomic Slope of 10 degrees provides maximum visibility and comfort,

reduces glare, thereby minimizing operator fatigue. See Figure 4-1.

3. Internal Depth of 25" provides necessary depth to work inside the

enclosure. See Figure 4-1.

4. Safety Glass Sash with Spring-Loaded Latch has a wiping seal and

features a spring-loaded latch to secure sash open for loading and cleaning. The sash must be down for normal operation. See Figure 4-1.

5. Utility Ports with Iris allows electrical cords and data cords to pass

through the back of enclosure without leaving a large hole. The enclosure ships with solid plugs. Iris plugs are included with the User’s Manual. See Figure 4-1 and 4-2.

6. Optional Guardian™ Airflow Monitor continuously monitors airflow.

An audio/visual alarm alerts the user to low airflow conditions. The

Guardian™ Airflow Monitor is an available option on all Purifier Filtered

PCR Enclosure models. See Figure 4-1 and 4-2.

7. Inherently Safe Impeller has a negative pressure plenum that surrounds

the positive pressure impeller so that if a leak should occur, the unfiltered air is captured and refiltered. See Figure 4-2.

8. Speed Control regulates the speed of the motorized impeller and is used

by the certifier to validate and adjust the downflow velocity. Located behind the front panel. See Figures 4-1 and 4-2.

9. Fluorescent Lamp is located above the work area, out of contact with the

clean air. A safety glass window beneath the lamp distributes the light evenly across the work surface. See Figure 4-1.

10. Filter Pressure Gauge indicates the pressure across the HEPA filter to

help predict HEPA filter loading. See Figure 4-1.

11. Filter Clamping Bolt evenly seals the HEPA filter to the frame of the

enclosure. See Figure 4-1 and 4-2.

12. Control Panel. The control panel, which is located above the sash,

contains the filter pressure gauge, control switches and the electronics. See Figure 4-1.

13. HEPA (High Efficiency Particulate Air) Filter is rated to remove

99.99% of all particles 0.3 micron in size. See Figure 4-2.

14. Lamp Ballast (Not Shown) for the fluorescent lamp is located behind the

control panel.

15. Variable Digital Timed UV (Ultraviolet) Lamp (Not Shown), allows the

operator to surface disinfect the work area of the enclosure when it is not in use. The UV light rays will not penetrate the plane of the sash and lower airfoil. The UV light timer is variable with eight settings in minutes of 5, 10, 15, 30, 60, 120, 240, and infinity. This provides extreme flexibility for

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Chapter 4: Performance Features and Safety Precautions

PCR protocol. It is easily changed by, selecting the time necessary located on the control panel. To operate, simply select the UV light option on the light switch.

16. UV Closure Panel provides protection when the UV lamp is on. The panel

must be in place for the UV lamp to work. See Figure 4-3.

17. Accessory Work Surface is dished and contoured to fit the dimensions of

the enclosures to contain spills.

18. Upper Diffuser Screen provides protection for the HEPA Filter and

promotes even distribution of downflow air. See Figure 4-2.

19. Prefilter (roughing) enhances the life of the HEPA filter by trapping large

particles. It should be replaced quarterly.

Safety Precautions

1. Although the enclosure has been engineered to maintain Class 5 air

conditions, caution should always be used while working. Prior to using the enclosure, check to make sure that the blower is operating and periodically ensure that air is flowing through the enclosure at its specified downflow velocity of 30 to 65 fpm. The use of an airflow monitor is recommended to alert the user to a problem with airflow.

2. Use good housekeeping in the enclosure at all times. Clean up spills

immediately. Periodically clean enclosure interior.

3. Do not overload the work surface with apparatus or work material.

4. The Purifier Filtered PCR Enclosures should never be used with

biohazardous materials, toxins, acids, or radionuclides. Air from the work area is dispersed directly into the laboratory from the enclosure. A qualified Safety Officer must carefully assess any risk associated with the use of the Purifier Filtered PCR Enclosure.

5. Always work with your hands as far back into the enclosure as possible.

Keep all materials and apparatus inside the lower air foil of the enclosure.

6. Do not work in this enclosure without the blower running.

7. Perchloric acid use in this enclosure is prohibited.

8. Radioactive materials are prohibited in this enclosure. Consult your Safety

Officer.

9. Avoid cross drafts and limit traffic in front of the enclosure. Air

disturbances created may disturb Class 5 conditions.

10. A qualified certification technician should test the enclosure before it is

initially used. The enclosure should be validated annually and whenever it is relocated.

11. The sash must remain in the down position while using the enclosure.

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Chapter 4: Performance Features and Safety Precautions

12. The HEPA filter provides Class 5 clean air conditions and only provides

product protection inside the enclosure. Do not use the enclosure for personnel protection.

13. Never use flammable gases or solvents in the enclosure. Use of an open

flame must be avoided in the enclosure. Open flames may disrupt the airflow patterns in the enclosure, burn the HEPA filter and damage the

filter’s adhesive. Gases under high pressure should not be used in the

enclosure as they may disrupt airflow patterns.

14. HEPA filters are only effective for entrapment of particulate matter.

15. The surface of the HEPA filter is fragile and should not be touched. Care

must be taken to avoid puncturing the HEPA filter during maintenance. If you suspect that a HEPA filter has been damaged DO NOT use the enclosure; contact a local certification agency or Labconco.

16. The HEPA filter will gradually accumulate airborne particulate matter from

the room. The rate of accumulation will depend upon the cleanliness of the room air, and the amount of time the enclosure is operating. With normal usage, the HEPA filters will last two to five years before requiring replacement.

17. Avoid direct exposure to ultraviolet (UV) radiation; NEVER work in the

enclosure when the UV light is on. Verify the sash is closed and the UV closure panel is in place before turning on the UV light.

18. Ensure that the enclosure is connected to an electrical service in accordance

with local and national electrical codes. Failure to do so may create a fire or electrical hazard. Do not remove or service any electrical components without first disconnecting the filtered enclosure from electrical service.

19. Increase the downflow velocity by adjusting the speed control of the

motorized impeller or change the HEPA filter when the downflow velocity falls below acceptable limits established by your Safety Officer.

20. Ensure only trained operators use the enclosure. New users should review

the User’s Manual and become familiar with the operation of the enclosure.

21. Proper operation of the enclosure depends largely upon the enclosure

location and the operator’s work habits. The enclosure should be located

away from traffic patterns, doors, fans, ventilation registers, fume hoods, and any other air-handling device that could disrupt its airflow patterns. Consult Chapter 2: Prerequisites and Chapter 3: Getting Started sections of this manual for further details.

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CHAPTER 5

APPROPRIATE APPLICATIONS FOR YOUR FILTERED PCR ENCLOSURE

Now that the installation of your Filtered PCR Enclosure is completed, you are ready to use it. Read this chapter to learn about:

1. Routine Daily Work Procedures.

2. Suitable Applications.

3. Appropriate HEPA Filter Applications, Suitability and Guidelines.

Routine Daily Work Procedures

Planning

 Arrange for minimal disruptions, such as room traffic or entry into the

room while the enclosure is in use.

Start-up

 Turn on blower and light. Turn off the UV light and remove the UV

closure panel.

 Only raise the sash for loading and cleaning.  Allow the enclosure’s blower to operate unobstructed for 1 minute.  Wear a long sleeved lab coat, rubber gloves and use protective eyewear.

Wear a protective mask if appropriate. Consult your Safety Officer for additional personal protective equipment recommendations.

 Wipe down interior surfaces with a mild household detergent or

disinfectant. DO NOT use abrasive cleaners, bleach or solvents.

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Chapter 5: Appropriate Applications for Your Filtered PCR Enclosure

Loading Materials and Equipment

 Load only the materials required for the procedure. Do not overload the

enclosure.

 Do not obstruct upper air diffuser screen.  Large objects should not be placed close together.  After loading, wait one minute to purge airborne contaminants from the

work area.

Work Techniques

 Keep all materials inside the lower air foil, and perform all operations as

far to the rear of the work area as possible.

 Segregate all clean and contaminated materials in the work area.

Final Purging

 Upon completion of work, the enclosure should be allowed to operate for

two to three minutes undisturbed, to purge airborne contaminants from the work area before shutting down the blower.

 Select the UV time for your PCR protocol, install the UV closure panel and

turn on the UV light to decontaminate the work area as determined by your Safety Officer.

Unloading Materials and Equipment

 All open trays, weigh vessels or containers should be covered before being

removed from the enclosure.

Suitable Applications

Suitable applications for the Purifier Filtered PCR Enclosures are based on guidelines established by the Institute for Environmental Sciences. Particulates are filtered by the HEPA filter before air is returned to the room. Biohazardous materials or other hazardous solids cannot be used with the Purifier Filtered PCR Enclosure, as air is drawn over the work surface and out of the enclosure. The Purifier Filtered PCR Enclosure only provides product protection and offers no personnel protection whatsoever.

Listed below are suitable applications for HEPA filters.

HEPA Filter Applications, Suitability and Guidelines

 Procedures traditionally performed on an open bench where a clean Class 5 air

environment is now required.

 The HEPA filtered enclosure provides product protection from particulate

matter. Because air from the enclosure enters the room during operation, this enclosure should only be used for operations requiring sample protection from environmental contamination.

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Chapter 5: Appropriate Applications for Your Filtered PCR Enclosure

 HEPA filters are only effective for entrapment of particulate matter. The

surface of the HEPA filter is fragile and should not be touched. Care must be taken to avoid puncturing the HEPA filter during installation. If you suspect that a HEPA filter has been damaged, DO NOT use the enclosure. See Chapter 6 for Replacing the HEPA Filter.

 The HEPA filter will gradually accumulate airborne particulate matter from

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CHAPTER 6

MAINTAINING YOUR FILTERED PCR ENCLOSURE

Monitoring airflow and changing the filters is the primary maintenance required. Certification and recertification is also reviewed in Chapter 6.

Review this chapter on maintenance for the following:

1. Routine Maintenance.

2. Determination of when to replace the HEPA filters.

3. How to install a new HEPA filter.

4. HEPA filter leak test.

5. Speed control adjustment and setting the downflow velocity.

6. Operating and calibrating the airflow monitors.

7. Initial certification.

8. Re-certification.

9. Fluorescent light replacement.

10. UV light replacement, if applicable.

11. Motorized impeller replacement.

12. Speed control replacement.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

HEPA

Routine Maintenance Schedule

Weekly

 Wipe down the interior surfaces of the enclosure with a disinfectant or

cleaner, depending upon the usage of the unit and allow to dry.

 Using a damp cloth, clean the exterior surfaces of the enclosure,

particularly the front and top to remove any accumulated dust.

 Operate the exhaust system, noting the airflow velocity out the front of the

enclosure using a source of visible smoke. Airflow monitors are recommended for constant monitoring.

Monthly (or more often as required)

 Determine the actual downflow velocity 6" below the upper diffuser of the

enclosure where the average reading should be at the specified velocity. (Use calibrated thermal anemometer or other approved apparatus). Airflow alarms are recommended for constant monitoring.

 All weekly activities.  Check downflow velocity. Increase speed control or change HEPA filter

when downflow velocity of the enclosure drops below the recommended speed for your facility or if the airflow alarm monitor alerts you. An airflow monitor is recommended.

Annually

 Replace UV lamps if equipped.  Verify proper operation of the UV closure panel switch.  Have the enclosure validated by a qualified certification technician. See

Certification and Recertification to follow in Chapter 6.

 All monthly activities.  Change prefilter.

When to Replace HEPA Filters

The HEPA filter in the Purifier Filtered PCR Enclosure gradually accumulates airborne particulate matter from the room. The rate of accumulation will depend upon the cleanliness of the room air, and the amount of operational time the enclosure is operating. In typical installations and usage, the HEPA filters will last two to five years before requiring replacement. Replace HEPA filters when downflow velocity drops below the recommended 30-65 fpm velocity and the speed control is adjusted to full speed. Replace HEPA filter if it fails the HEPA Filter Leak Test in Chapter 6.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

ATTENTION: The filter clamp bolts should only be tightened enough to ensure a proper seal at maximum tightness. The filter gasket should be compressed 50% or less.

How to Install a New HEPA Filter

NOTE: Only a qualified certifier should service the HEPA filter. After the HEPA

filter is replaced, the enclosure MUST be certified. See Figure 6-1.

1. Unplug the enclosure and remove the prefilter located on top.

2. Remove the front panel by loosening the two screws that secure it.

3. Using a 9/16" deep socket, loosen the filter clamp bolts located on top.

Refer to Figure 6-1.

4. With the clamp bolts loosened, the HEPA filter-clamping frame should be

clear of filter. Carefully pull the filter straight out of the enclosure and discard properly.

5. Cover the surface of the new HEPA filter gasket with a light coating of

silicone grease, if desired.

6. Install the new HEPA filter by pushing it straight into the cabinet, ensuring

that it is correctly oriented with the gasket facing down on the exhaust side of the enclosure.

7. Tighten the clamp bolts uniformly until the filter gasket is properly

compressed against the frame. Inspect the seal thoroughly before proceeding.

8. Reinstall the front panel and prefilter.

9. Plug the enclosure in and have it check for leaks and downflow velocity.

Do NOT contact blower wheel while still in motion.

NE PAS être en contact avec la roué du ventilateur tant qu’il est

en marche.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Prefilter

Filter

Clamp Bolt

HEPA Filter

Sealing Surface

HEPA Filter

Gasket Side

Down as Shown

Gasket

Front Panel

Speed Control

Screw

HEPA Filter Changing Diagram & Filter Leak Test Diagram

Adjustment

Figure 6-1

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Chapter 6: Maintaining Your Filtered PCR Enclosure

HEPA

HEPA Filter Leak Test

Purpose

After installing the new HEPA filter, the HEPA filter should be leak checked. This test is performed to determine the integrity of the HEPA filter, the filter housing, and the filter seal. Leak testing must be done by a qualified technician with calibrated equipment. Remove the upper perforated diffuser located inside the enclosure by using a Phillips screwdriver to unfasten the screws (see Figure 6-

1). The filter passes the leak test at .01% or better. Reference Leak Testing and Photometer scanning from the Institute of Environmental Services (IES-RPCC001.3).

Apparatus

1. An aerosol photometer ATI model 2D, 2E, 2G or equivalent. Air

Techniques Hamilton Associates Inc., 11403 Cron Ridge Dr., Owings Mills, MD 21117

2. One aerosol generator of the Laskin nozzle(s) type. An aerosol of mineral

oil or suitable liquid shall be created by flowing air through it. The compressed air supplied to the generator should be adjusted to a pressure of 10± 1 psig. during operation. Air Techniques Inc. Model TDA-4A or equal. One nozzle at 10 psig is (67.5 cfm x 100ug/l)/(Vol. of air), For the 2' at 55 fpm or 230 cfm, one nozzle @10 psig is 6750/230 cfm = 30 ug/l. For the 3' at 55 fpm or 340 cfm, one nozzle @ 10 psig is 20 ug/l. For the 4' at 55 fpm or 460 cfm, one nozzle at 10 psig is 15 ug/l.

3. Mineral oil (Catalog #1491400).

4. Sampling Nozzle, Rectangular 1/2" x 3-1/4", Air Techniques, Inc.

Procedure

For the ATI 2G Photometer

1. Turn on the photometer and allow it to operate for a minimum of 5 minutes.

Leave the valve in the “CLEAR” setting.

2. Press the “ENTER” keypad. Press the “REF” keypad.

3. The display will display “P1” for approximately 1 second, and then display a

numerical value.

4. Using the “^” or “ν” keypads, respectively, increase or decrease the numerical

value until it equals 30 (2'), 20 (3'). 15 (4') for the Purifier Filtered PCR enclosure at 55 fpm downflow velocity.

5. Press the “ENTER” Keypad. The photometer will scan for 15 seconds, and

then the “0” keypad will flash. Press the “Enter” keypad. The unit will scan for 5 seconds, the display will read “0000,” and the unit will sound a confirming

tone.

6. Set the valve to “DOWNSTREAM.” Place the palm of your hand over the

sampling port of the pistol. There should be a strong vacuum at this port. If the vacuum is weak, contact Air Techniques Hamilton Associates.

7. Turn the enclosure on and let it operate for a minimum of 5 minutes.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Options

8. If necessary, adjust the speed control of the enclosure to maintain the following

downflow airflows at 55 fpm; 2' (230cfm), 3' (340 cfm), 4' (460 cfm.)

9. Position the aerosol generator discharge in the intake of the prefilter on top of

the enclosure.

10. Start the aerosol generator (Pressure to be 10 +/- 1 PSIG). Ensure that one

Laskin nozzle is in the “open” position.

11. Allow the generator to operate for a minimum of 15 seconds. Scan the

downstream exhaust side of the HEPA filter by passing the sampling nozzle of the gun in slightly overlapping strokes over the entire surface the filter, with the sampling port not more than 1 inch from the surface of the filter media. Scan the entire periphery of the filter and the gasket between the filter frame and the enclosure frame. Scanning shall be done at a traverse rate of not more than 2 inches per second.

Acceptance

Aerosol penetration shall not exceed 0.01 percent measured by the photometer.

Setting the Downflow Velocity with the Speed Control Adjustment

1. Remove the front panel by loosening the (2) Phillips screws on top that

secure the front panel.

2. The speed control is located on the electrical subassembly located behind

the switched control panel and below the front panel. See Figure 6-1.

3. Adjust the speed control with a small Phillips screwdriver by turning the

screw counterclockwise to increase blower speed or clockwise to decrease the blower speed. The speed control is very sensitive, so proceed with caution.

4. Measure the downflow velocity per the averaging technique outlined in

Chapter 3 and adjust the speed control slowly for the desired speed. Allow the speed to stabilize and re-measure the downflow velocity to confirm.

5. Replace the front panel and tighten the screws.

Calibrate and Operate the Airflow Monitor

Optional Guardian Airflow Monitor

Refer to Figure 6-2 for operation and calibration.

Labconco Airflow Monitor / Airflow Switch Operation

The Guardian Airflow Monitor consists of a circuit board and an airflow switch. This switch indicates airflow as safe or low. It does not provide an actual velocity, but a small setscrew in the back of the sensor can adjust the airflow level that it classifies as “good/safe” or “low/alert.”

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Chapter 6: Maintaining Your Filtered PCR Enclosure

The circuit board provides power to the sensor and also contains a “safe (green)” and “alert (red)” airflow LED indicators, as well as a “SILENCE ALARM” button

to quiet the audio alarm. When first powered up, the Printed Circuit Board (PCB) will light both red and green LED indicators and sound the alarm to indicate it is working. After 5 seconds, the air monitor will indicate either good or bad airflow based on what the connected airflow switch detects. For low airflow, the unit will wait for 10 seconds after low indications before it sounds both the audio alarm and

the red “alert” LED indicator. If the “SILENCE ALARM” button is pressed, the

audio alarm will be silenced, but the red “alert” LED will remain on. The alarm is

silenced indefinitely unless an airflow change is detected. If safe airflow is later

detected for 10 seconds, the green “safe” LED will be lit and the “alert” (red) LED

will be shut off. At any time the airflow is safe/good, one can press the SILENCE ALARM test button and the audio alarm and the red LED will turn on as long as this button is held down. The PCB has also a two-pin connector for use as an external output with isolated relay contacts that close when the red/alert LED is lit

(low airflow). These relay contacts are not affected by the “SILENCE ALARM”

button.

The PCB is mounted behind the front panel using standoffs and an appropriate label is used to highlight the “SILENCE ALARM” button with clear areas for the red and green LED’s.

By special order, the PCB can be prepared as a factory special with an additional connector for the following external inputs, and having the following possible functions:

 External Alarm allows an external signal to sound the alarm from the building

airflow system of a failed condition.

 Alarm Disable allows an external signal to prevent a “low” airflow alarm from

occurring.

 Night Setback allows an external signal to prevent a “low” airflow alarm from

occurring (not any different from Alarm Disable above other than the terminology.)

 Contact Labconco for ordering information on this special PCB.

Calibration

1. Ensure the flow switch and alarm circuit board are installed and

operational.

2. Allow the enclosure to operate for at least two minutes.

3. If factory installed, the monitor will alarm at 30±10 fpm downflow velocity

with the average outflow velocity set at 85±10 fpm.

4. To change the factory setting, set the downflow velocity to the desired

alarm condition using the speed control adjustment procedure outlined in Chapter 6. Once the alarm condition is set, use a small screwdriver to turn the adjustment screw on the airflow switch counterclockwise (facing the screw) until the “low” airflow red LED lights and the audible flow alarm sounds.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Enclosure Operating

Downflow Speed

Alarm Condition Set Point

Speed

55 ± 10 fpm

25-35 fpm

45 ± 10 fpm

25-35 fpm

5. Adjust the downflow velocity to the nominal operating point required. The

green “safe” LED should light.

6. Over time the HEPA filter will load and eventually slow the downflow

velocity. Once the alarm condition is met, simply increase the speed control as outlined in Chapter 6 or replace the HEPA filter if the speed control is maximized.

7. The table below lists typical alarm conditions based on normal operating

conditions. Typical alarm conditions are set at downflow velocities of 10 to 30 feet per minute below the normal operating conditions due to supply air and exhaust air fluctuations, as well as room air cross drafts. Consult your Safety Officer or Laboratory Manager for proper operating speeds.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Light Reflector

Speed Control

Airflow Monitor

Board

Airflow Switch

with Adjustment

Screw

Control Panel

Green “Safe” LED

Silence Alarm Button

with Test/Reset

Red “Alert” LED

with Audible Alarm

Figure 6-2

Guardian Airflow Monitor with Airflow Switch

LED Circuit

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Initial Certification

The Purifier Filtered PCR Enclosure has been certified at the factory for an average downflow velocity of 40-55 fpm along with the HEPA Filter Leak Test. The Purifier Filtered PCR Enclosures should be certified for the proper downflow velocity required by your Safety Officer. It is also a conservative recommendation to perform the HEPA Filter Leak Test again should there be any damage caused during transport.

Re-Certification

Under normal operating conditions, the Purifier Filtered PCR Enclosure should be recertified at least annually. The certifier should perform the minimum following tests:

 Downflow Velocity Test  HEPA Filter Leak Test

In addition, the following tests should also be performed at the user’s discretion:

 Measure of Line Voltage and Current  Smoke Test to determine proper airflow patterns  Lighting Intensity Test (when appropriate)  Noise Level Test (when appropriate)  Vibration Test (when appropriate)

Fluorescent Light Replacement

1. Disconnect the power.

2. Locate the small light reflector located under the control panel.

3. Remove the light reflector by removing two Phillips screws on the bottom

of the light reflector.

4. Rotate and remove the old fluorescent lamp.

5. Reinstall the new fluorescent lamp and light reflector in reverse order.

6. Power the unit up and try the new fluorescent lamp.

UV Light Replacement

1. Disconnect the power.

2. Locate the UV lamp located inside the enclosure.

3. Rotate and remove the old UV lamp.

4. Reinstall the new UV lamp.

5. Power the unit up and try the new UV lamp (UV closure panel must be

installed).

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Motorized Impeller Replacement

The motorized impeller must be replaced as a complete unit. When the motorized impeller is replaced, the capacitor should also be replaced. See Appendix A for Replacement Parts Diagram. See Figure 6-3 for an isometric view of the motorized impeller plenum assembly. The HEPA filter rests below the motorized impeller assembly.

Do NOT contact blower wheel while still in motion.

NE PAS être en contact avec la roué du ventilateur tant qu’il est en marche.

1. Unplug the enclosure from the electrical outlet.

2. Remove HEPA filter per the HEPA filter removal procedure outlined earlier in

this chapter.

3. Consult the wiring diagram in Appendix C of the manual and disconnect all the

wires of the motorized impeller. Be sure to connect wires on the new motor in the same way the old motor was wired. The 3' and 4' enclosures have two motorized impellers.

4. Remove four screws in each motorized impeller.

ATTENTION: High-speed blower. Never operate impeller with housing off.

5. Replace each capacitor with a new one of equal voltage and capacity.

6. Reassemble each new motorized impeller by reversing the assembly steps.

7. Perform a HEPA filter leak check.

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Chapter 6: Maintaining Your Filtered PCR Enclosure

2' Model

Mounting Screws

Impeller

HEPA Filter

Capacitor

Mounted here on

Motorized

Impeller

Wire Access

Hole

Impeller

Mounting Bracket

Hardware

Front Panel

Wire Access

Holes

Motorized

Impellers

3' or 4' Models

for Motorized

Electrical

Subassembly

located behind

the Control

Panel

Figure 6-3

Motorized Impeller Replacement

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Chapter 6: Maintaining Your Filtered PCR Enclosure

Speed Control Replacement

1. Remove the bracket that the speed control is attached to. See Figures 6-1, and

6-2.

2. Remove the two screws holding the speed control using a Phillips screwdriver.

Refer to Appendix A for Replacement Parts Diagram.

3. Disconnect all wires leading to the speed control. Connect wires on new speed

control in the same position as the old speed control.

4. Reassemble to the system in the same position and with the same screws that

were removed earlier.

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Catalog #

Description

Dimensions (W x D x H)

3909900

Black, 2-foot wide

24" x 29" x 1"

3909901

Black, 3-foot wide

36" x 29" x 1"

3909902

Black, 4-foot wide

48" x 29" x 1"

3909903

Gray, 2-foot wide

24" x 29" x 1"

3909904

Gray, 3-foot wide

36" x 29" x 1"

3909905

Gray, 4-foot wide

48" x 29" x 1"

CHAPTER 7

ACCESSORIZING AND

MODIFYING YOUR FILTERED PCR ENCLOSURE

There are several ways to accessorize and modify the Filtered PCR Enclosure for your individual requirements. These include the addition of accessory work surfaces, airflow monitors, filters, and storage cabinets or stands.

1. Work Surfaces

An optional dished work surface is available to attach to the Filtered PCR Enclosure.

Dished work surfaces are contoured to fit the dimensions of the filtered enclosures to contain spills. Epoxy is chemical resistant. See Figure 3-1 for installation.

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Chapter 7: Accessorizing and Modifying Your Filtered PCR Enclosure

Description

Order Part #’s

Individual Parts Required

Guardian Airflow Monitor 115V or 230V

1 ea.

3811500

Airflow Monitor Printed Circuit Board

1 ea.

3910700

Airflow Sensor w/ Connector

2 ea.

1885405

#6-32 x .31” Phillips machine screw to mount Airflow Monitor Circuit Board

2 ea.

1891808

#6-32 x .50” Phillips thread cutting screw to mount Airflow Sensor

1 ea.

3935501

Upper Diffuser w/ Air cutout 2'

1 ea.

3935503

Upper Diffuser w/ Air cutout 3'

1 ea.

3935505

Upper Diffuser w/ Air cutout 4'

Width

Catalog #

Size

3707900

18" x 18" x 3.31"

3707901

30" x 18" x 3.31"

3707902

42" x 18" x 3.31"

Filter

Catalog #

Qty.

3935700

3935701

3935702

2. Guardian™ Airflow Monitor

The Guardian Airflow Monitor allows you to continuously monitor airflow through the enclosure. The Guardian monitor can be placed on any Purifier Filtered PCR Enclosure.

3. Accessory Filters HEPA Filter

HEPA Filter is 99.99% efficient on particles 0.3 micron.

Prefilters (Roughing) A prefilter to extend HEPA filter life.

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Chapter 7: Accessorizing and Modifying Your Filtered PCR Enclosure

SOLVENT

ACID

Size/Description

Dual Doors

Right Hinge

Left Hinge

Dual Doors

Right Hinge

Left Hinge

48"

9902000

9901000

36"

9902100

9901100

30"

9902200

9901200

24" - 9902300

9902400

9901300

9901500

18" - - - -

9901400

9901600

12" - - - - - -

48" w/Self-Closing Doors

9903000

- - - - -

36" w/Self-Closing Doors

9903100

- - - - -

30" w/Self-Closing Doors

9903200

- - - - -

24" w/Self-Closing Doors

9903300

9903400

- - -

24" ADA

9906000

9906100

9905000

9905200

24" ADA w/Self-Closing Doors

9906200

9906300

- - -

18" ADA - - - -

9905100

9905300

12" ADA - - - - - -

STANDARD BASE

VACUUM PUMP

Size/Description

Dual Doors

Right Hinge

Left Hinge

Dual Doors

Right Hinge

Left Hinge

48"

9900000

- - - - -

36"

9900100

- - - - -

30"

9900200

- - - - -

24" - 9900300

9900600

- - -

18" - 9900400

9900700

9907000

9907100

12" - 9900500

9900800

- - -

48" w/Self-Closing Doors

- - - - -

36" w/Self-Closing Doors

- - - - -

30" w/Self-Closing Doors

- - - - -

24" w/Self-Closing Doors

- - - - -

24" ADA

9904000

9904300

- - -

24" ADA w/Self-Closing Doors

- - - - -

18" ADA

9904100

9904400

- - -

12" ADA

9904200

9904500

- - -

Base Stands

3747000

Telescoping Base Stand with Fixed Feet

24" w x 25.5" d x 27. 5" to 33.5" h

3746701

Telescoping Base Stand with Fixed Feet

36" w x 29.25" d x 27.5" to 33.5" h

3746702

Telescoping Base Stand with Fixed Feet

48" w x 29.25" d x 27.5" to 33.5" h

3747010

Telescoping Base Stand with 5" Casters

24" w x 25.5" d x 27. 5" to 33.5" h

3746711

Telescoping Base Stand with 5" Casters

36" w x 29.25" d x 27.5" to 33.5" h

3746712

Telescoping Base Stand with 5" Casters

48" w x 29.25" d x 27.5" to 33.5" h

4. Storage Cabinets and Stands

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PROBLEM

CAUSE

CORRECTIVE ACTION

Class 100 (ISO Class

5) air conditions are not present inside the enclosure.

Improper user techniques for the enclosure.

See “Certifying the Enclosure” Chapter 3 and “Safety Precautions” Chapter 4

sections in the manual. (Ref. Appendix D).

External factors are disrupting the enclosure airflow patterns.

See “Location Requirements” Chapter 2, “Certifying the Enclosure” Chapter 3, and “Safety Precautions” Chapter 4 sections of

this manual. (Ref. Appendix D).

Enclosure has improper downflow velocity.

Have enclosure certified and check exhaust system. Check HEPA filters for loading. Adjust enclosure speed control. Enclosure should have an average downflow velocity of 30-65 fpm.

Blower won’t

operate.

Unit not plugged into outlet.

Plug the enclosure into appropriate electrical service.

CHAPTER 8 TROUBLESHOOTING AND

SERVICER OPERATING LOG

Refer to the following table if your Filtered PCR Enclosure fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance.

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Chapter 8: Troubleshooting and Servicer Operating Log

PROBLEM

CAUSE

CORRECTIVE ACTION

Blower won’t

operate.

Circuit breaker(s) or Ground Fault Interrupter.

Reset circuit breaker. Blower wiring is disconnected.

Inspect blower wiring.

Blower switch is defective.

Replace switch.

Motorized impeller or blower is defective.

Replace motorized impeller or blower. See Chapter 6.

Poor internal Class 5 air conditions.

Enclosure sash not closed.

Close sash. HEPA filter clogged.

Replace HEPA filter or increase speed.

Blower and lights won’t operate.

Unit not plugged into outlet.

Plug enclosure into appropriate electrical service.

Circuit breaker(s) tripped.

Reset or replace circuit breaker.

Light does not work.

Lamp not installed properly.

Inspect lamp installation.

Lamp wiring disconnected.

Inspect lamp wiring. Defective lamp.

Replace lamp.

Light switch is defective.

Replace light switch.

Defective electronic ballast.

Replace ballast.

UV closure panel not installed .

Install UV closure panel (UV lights only).

Magnetic switch for UV closure panel defective.

Replace switch (UV lights only). UV timer circuit board defective.

Replace circuit board (UV lights only).

UV light timer does not work

No power. No lights. No display

Power cable to UV light timer circuit board is disconnected. Verify connection. Replace circuit board for UV light timer if it malfunctions.

Airflow monitor malfunction

No power. No lights. No display.

Power cable to circuit board is disconnected and needs to be connected. Sensor cable needs to be connected. Verify that all airflow monitor interface cables are connected. Check fuses on enclosure.

No audible alarm.

Alarm has been temporarily silenced using “SILENCE ALARM.”

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Chapter 8: Troubleshooting & Servicer Operating Log

PROBLEM

CAUSE

CORRECTIVE ACTION

Airflow monitor malfunction (Continued)

Wrong alarm set point.

Airflow monitor was not properly adjusted. Repeat calibration steps outlined in this manual in Chapter 6.

Constant audible alarm.

Check airflow and calibration of airflow monitor. See Chapter 6.

Continuous alarm.

Check the downflow velocity of the enclosure as the airflow of the system may have changed. If incorrect, adjust the speed control to increase downflow velocity. The HEPA filter may have become loaded. If downflow velocity is correct, calibrate the airflow monitor outlined in this manual in Chapter 6.

Monitor alarms; air way to airflow monitor sensor is blocked by insects, dust or debris.

Lightly clean the airway with clean air. Be careful not to touch sensitive electrical components.

Audible disable will not stay operational.

An alarm condition must be continuously present before the audible alarm can be silenced. If flow conditions fluctuate near the alarm set point, the airflow monitor will automatically reset it. Action should be taken to bring the enclosure airflow into proper operating parameters or adjust the alarm set point lower.

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Chapter 8: Troubleshooting and Servicer Operating Log

Date

Comments

Customer Name

Model Number

Date Installed

Serial Number

Unit Location

Service Operating Record Log

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APPENDIX A

FILTERED PCR ENCLOSURE

COMPONENTS AND REPLACEMENT PARTS

The components that are available for your Filtered PCR Enclosure are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service.

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Appendix A: Components and Replacement Parts

Item

Qty.

Part Number

Description

1 1 3911000

Glass, Side

2A 1 3911900

Glass, Sash 2'

2B 1 3911901

Glass, Sash 3'

2C 1 3911902

Glass, Sash 4'

3A 1 3994900

Sash Assembly, 2'

3B 1 3994901

Sash Assembly, 3'

3C 1 3994902

Sash Assembly, 4'

4A 1 3905600

Lower Air Foil, 2'

4B 1 3905601

Lower Air Foil, 3'

4C 1 3905602

Lower Air Foil, 4'

5A 1 3907400

Bracket, Air Foil R.H.

5B 1 3907401

Bracket, Air Foil L.H.

6 2 1554500

Stop, Sash Bumper.

7A 1 6916500

Latch, Sash

7B 1 3906700

Bracket, Latch

7C 1 1927403

Spring, Compression Latch

7D 2 1893206

Screw, 8-32 x .38" Lg. Phillips Pan S.S. Type F

8 1 3945300

Printed Circuit Board, UV Light Timer

9A 2 1889316

Screw, 10-24 x 1.00 PH Phil S.S.

9B 2 1912108

Washer, Plastic .194 ID. x .380 OD

9C 2 7868402

Bushing, Spacer .31 x .63

1.9 Ft

6913700

Wiper, Sash Seal

11A 2 1936800

Bushing, Heyco closed 1.50 Dia.

11B 2 1934601

Bushing, Heyco w/ Flex Shutter 1.50 Dia.

12A 2 3940500

Hinge, outer

12B 2 3940501

Hinge, Inner

12C 4 1591606

Rubber Bumper

12D 2 3939600

2' and 4' Sash Closure Door

12E 2 3939601

3' Sash Closure Door

12F 2 3940700

Extension Bi-Fold Door 3'

12G 2 3940701

Extension, Bi-Fold Door 4'

13A 1 3907700

Reflector, Light 2'

13B 1 3907701

Reflector, Light 3'

13C 1 3907702

Reflector, Light 4'

13D 2 1889308

Screw, Mach. 10-24 x .50 PH Phillips

14A 1 3708300

Lamp, Fluorescent 2' Enclosure (F15T8-SP35)

14B 1 9721902

Lamp, Fluorescent 3' Enclosure (F17T8)

14C 1 9721901

Lamp, Fluorescent 4' Enclosure (F25T8)

15A 1 1270100

Lamp, UV 2' or 3' Enclosure (G15T8)

15B 1 1271300

Lamp, UV 4' enclosure (G30T8)

16A 1 3911700

Bracket, Right Reflector Support

16B 1 3911701

Bracket, Left Reflector Support

16C 4 1893108

Screw, #8 x 1/2 AB Hex Washer Head

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Appendix A: Components and Replacement Parts

Item

Qty.

Part Number

Description

17A 1 3937910

Wiring Harness Main, 115V, 2'

17B 1 3937911

Wiring Harness Main, 230V, 2'

17C 1 3938010

Wiring Harness Main, 3' and 4' 115V

17D 1 3938011

Wiring Harness Main, 3' and 4' 230V

18A 1 3811500

Guardian Airflow Monitor Printed Circuit Board

18B 2 1885405

Screw Mach #6-32 x .31 PH Phil. S.S.

19 1 3936700

Label, Front Set

20A 1 3910700

Airflow Switch

20B 2 1891808

Screw, Thread Cutting #6-32 x .50 PH

22A 1 1307000

Switch, Rocker (2 position) - Blower

22B 1 1306300

Switch, Rocker (3 position) – UV Light Only

23A 1 3904800

Front Panel, 2'

23B 1 3904801

Front Panel, 3'

23C 1 3904802

Front Panel, 4'

23D 1 4472400

Screw, Thread cut 10-24 x .50 Type F PH Phillips

24A

1, 1 or 2

3934500

Impeller, Motorized 2', 3' or 4' Enclosure, 115V

24B

1, 1 or 2

3934501

Impeller, Motorized 2', 3' or 4' Enclosure, 230V

24C 2 3934600

Impeller, Motorized 4', 115V (older units prior to 7-1-05)

24D 2 3934601

Impeller, Motorized 4', 230V (older units prior to 7-1-05)

25A 1 1306900

Capacitor, 2', 115V 10 MFD

25B

1 or 2

1306900

Capacitor, 3' or 4', 115V 10 MFD

25C 2 1306600

Capacitor, 4', 115V 15 MFD (prior to 7-1-05)

25D 2 1306800

Capacitor, 2', 230V 4 MFD (effective 2MFD)

25E

2 or 4

1306800

Capacitor, 3' or 4', 230V 4 MFD (effective 2MFD)

25F 2 1306800

Capacitor, 4', 230V 4 MFD (prior to 7-1-05)

26A 1 1295510

Ballast, Fluorescent 2', 115V

26B 1 1295510

Ballast, Fluorescent 2', 230V

26C 1 1294000

Ballast, Fluorescent 3' or 4', 115V or 230V

27A 1 1295510

Ballast, UV 2' or 3', 115V

27B 1 1295510

Ballast, UV 2' or 3', 230V

27C 1 1295700

Ballast, UV 4', 115V

27D 1 1295700

Ballast, UV 4', 230V

28A 1 3922100

Speed Control, 115V

28B 1 3922101

Speed Control, 230V

29A 1 3935500

Upper Diffuser Screen 2' (Internal)

29B 1 3935502

Upper Diffuser Screen 3' (Internal)

29C 1 3935504

Upper Diffuser Screen 4' (Internal)

29D 1 3935501

Upper Diffuser w/ Air cutout 2'

29E 1 3935503

Upper Diffuser w/ Air cutout 3'

29F 1 3935505

Upper Diffuser w/ Air cutout 4'

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Appendix A: Components and Replacement Parts

Item

Qty.

Part Number

Description

1893712

Screw, #10 x .75 PH Phil. Thd. Forming

31A 2 1881196

Capscrew, 3/8 –16 x 6.0 Lg. Hx. Hd.

31B 4 1911018

Washer, Flat 3/8

31C 2 1906925

Nut, Hex 3/8 Nylon Lock

32A 1 3707900

HEPA Filter (2') 18 x 18 x 3.32 w/ Gasket

32B 1 3707901

HEPA Filter (3') 30 x 18 x 3.32 w/ Gasket

32C 1 3707902

HEPA Filter (4') 42 x 18 x 3.32 w/ Gasket

35A 1 1333800

IEC Power Inlet, 115V

35B 1 1305800

Power Cord, 115V, Right Angle

35C 1 1305900

Power Cord, 230V, Right Angle

35D 1 1339200

Receptacle, RFI Inlet 230V

36A

1 - (115V)

1327204

Circuit Breaker, 5 AMP

36B

2 - (230V)

1327200

Circuit Breaker, 3 AMP

40A 1 1952500

Gauge, Pressure Mini Helic II

40B 1 3667400

Bracket, Gauge

40C 2 1905617

Nut, #10-24 KEPS

40D 2 1551700

Tubing, 3/16" ID x 5/16" OD, PBC 7.00" Lg.

40E

2, 1" Long

3788200

Snubber, Filter Pressure Gauge

41A 1 3560510

Panel, UV Closure 2 Ft.

41B 1 3560511

Panel, UV Closure 3 Ft.

41C 1 3560512

Panel, UV Closure 4 Ft.

42 2 1907002

Tape, hook and latch, fastening

43 1 1322800

Switch assembly, magnetic

Product Service 1-800-522-7658

Appendix A: Components and Replacement Parts

Fluorescent

15 7 14

35, 36

Not

Shown

1 6 3

12, Not Shown

8, UV Light Timer Located Behind Control Panel

Figure A-1

Replacement Parts, Purifier Filtered PCR

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APPENDIX B DIMENSIONS

Figure B-1

Purifier Filtered PCR Enclosure

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APPENDIX C FILTERED PCR ENCLOSURE

SPECIFICATIONS

This Appendix contains technical information about all Purifier Filtered PCR Enclosures including electrical specifications and environmental operating conditions.

Electrical Specifications

 5 Amps, 115V, 60 Hz  3 Amps, 230V, 50/60 Hz

Environmental Conditions

 Indoor use only.  Maximum altitude: 6562 feet (2000 meters).  Ambient temperature range: 41° to 104°F (5° to 40°C).  Maximum relative humidity: 80% for temperatures up to 88°F (31°C),

decreasing linearly to 50% relative humidity at 104°F (40°C).

 Main supply voltage fluctuations not to exceed ±10% of the nominal

voltage.

 Transient over-voltages according to Installation Categories II (Over-

voltage Categories per IEC 1010). Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed.

 Used in an environment of Pollution degrees 2 (i.e., where normally only

non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.

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APPENDIX D REFERENCES

Many excellent reference texts and booklets are currently available. The following is a brief listing:

Bryan, D., and R.C. Marback. 1984. Laminar-airflow equipment certification: What the Pharmacist needs to Know. American Journal of Hospital Pharmacy. 41.1343-1348.

General Services Administration. 1988. FED-STD-209E Federal Standard – Clean Room and Work Station Requirements, Controlled Environment. Washington D.C.

IES Recommended Practice: IES-RP-CC-001-86. Recommended Practice for HEPA Filters. Mt. Prospect, IL; Institute for Environmental Sciences. 1986.

IES Recommended Practice: IES-RP-CC-001-96. Laminar Flow Clean Air Devices. Mt. Prospect, IL: Institute for Environmental Sciences. 1986.

IES Document: IES-CC-011-85-T. A Glossary of Terms and Definitions Related to Contamination Control. Mt. Prospect, IL: Institute for Environmental Sciences. 1985.

ISO 14644-1: 1999. Cleanrooms and Associated Controlled Environment International Organization for Standardization.

Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR, Journal of Chemical Microbiology. May 2000. p. 1747-1752. Vol. 38, No. 5.

Elimination of Contaminating DNA within Polymerase Chain Reaction Reagents: Implications for a General Approach to Detection of Uncultured Pathogens, Journal of Clinical Microbiology. Mar. 1993. p. 646 – 652. Vol. 31, No. 3.

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Labconco Purifier Filtered PCR Enclosures User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual