Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com
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User’s Manual
Models
3970202, 3970205, 3970222, 3970225,
3970302, 3970305, 3970322, 3970325,
3970402, 3970405, 3970422, 3970425
Please read the User’s Manual before operating the equipment.
Purifier® Filtered PCR Enclosures
Copyright © 2004, 2007, 2012, 2013, 2014, 2015 Labconco Corporation. All rights reserved.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment. Glassware is not warranted from breakage when dropped or mishandled.
The warranty for Purifier® Filtered PCR Enclosures will expire one year from date of installation or
two years from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable
and only applies to the owner (organization) of record.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.
The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #3938201, Rev. C
ECO J457
ORIGINAL INSTRUCTIONS
TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION 1
ISO Class 5 Definition 2
Polymerase Chain Reaction Definition 2
About This Manual 3
Typographical Conventions 4
CHAPTER 2: PREREQUISITES 5
Support, Vibration & Movement Requirements 5
Location and Air Current Requirements 6
Airflow Specifications 6
Electrical Requirements 7
Space Requirements 7
CHAPTER 3: GETTING STARTED 8
Unpacking Your Enclosure 9
Installing the Filtered PCR Enclosure on a Supporting Structure
and Work Surface 9
Verifying HEPA Filter Integrity 11
Connecting the Electrical Supply Source to the Filtered PCR
Enclosures 11
Setting the Downflow Velocity with Speed Control 12
Validating the Filtered PCR Enclosure 12
Sealing the Filtered PCR Enclosure to the Work Surface 13
CHAPTER 4: PERFORMANCE FEATURES AND
SAFETY PRECAUTIONS 14
Components 15
Safety Precautions 19
CHAPTER 5: APPROPRIATE APPLICATIONS FOR YOUR
FILTERED PCR ENCLOSURE 21
Routine Daily Work Procedures 21
Suitable Applications 22
HEPA Filter Applications, Suitability & Guidelines 22
CHAPTER 6: MAINTAINING YOUR FILTERED PCR
ENCLOSURE 24
Routine Maintenance Schedule 25
When to Replace HEPA Filters 25
How to Install a New HEPA Filter 26
HEPA Filter Leak Test 28
Setting the Downflow Velocity with the Speed Control Adjustment 29
Calibrate and Operate the Airflow Monitor 29
Initial Certification 33
Re-Certification 33
Fluorescent Light Replacement 33
UV Light Replacement 33
Motorized Impeller Replacement 34
Speed Control Replacement 36
CHAPTER 7: ACCESSORIZING & MODIFYING YOUR
FILTERED PCR ENCLOSURE 37
CHAPTER 8: TROUBLESHOOTING & SERVICER
OPERATING LOG 40
APPENDIX A: FILTERED PCR ENCLOSURE COMPONENTS
AND REPLACEMENT PARTS 44
APPENDIX B: DIMENSIONS 49
APPENDIX C: FILTERED PCR ENCLOSURE
SPECIFICATIONS 50
APPENDIX D: REFERENCES 51
1
CHAPTER 1
INTRODUCTION
Congratulations on your purchase of a Labconco Purifier® Filtered PCR
Enclosure. Your enclosure provides a particulate free environment for Polymerase
Chain Reaction experiments. It is the result of Labconco’s more than 30 years
experience in manufacturing filtered enclosures.
These enclosures will effectively provide product protection from airborne
particulate matter. During operation, room air is drawn through the prefilter on
top, forced through the blower and then through the HEPA filter and diffuser. The
clean air in the work area in the enclosure meets or exceeds ISO Standard Class 5
conditions defined per ISO 14644-1 and Class 100 conditions as defined by
Federal Standard 209E. The enclosure is set to deliver nominal downward airflow
of 40-55 feet per minute. Each enclosure uses a single HEPA filter, which is rated
at least 99.99% efficient for 0.3-micron particles. The Purifier Filtered PCR
enclosures offer many unique features to enhance performance. A variable solidstate digital timer provides a UV light to destroy contaminating DNA and RNA in
the work area. The UV light automatically turns off to prepare for the next
experiment. To take full advantage of all features, please acquaint yourself with
this manual and keep it handy for future reference. If you are unfamiliar with how
Filtered PCR Enclosures operate, please review Chapter 4: Performance Features
and Safety Precautions before you begin working. Even if you are an experienced
user, please review Chapter 5: Using Your Filtered PCR Enclosure, which
describes the enclosure features.
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Chapter 1: Introduction
2
Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.
ISO
classification
number (N)
Maximum concentration limits (particles/m3 of air) for particles equal to and
larger than the considered sizes shown below (concentration limits are
calculated in accordance with 3.2 of Standard 14644-1)
0.1 µm
0.2 µm
0.3 µm
0.5 µm
1 µm
5 µm
ISO Class 1
10 2
ISO Class 2
100
24
10 4
ISO Class 3
1 000
237
102
35 8
ISO Class 4
10 000
2 370
1 020
352
83
ISO Class 5
100 000
23 700
10 200
3 520
832
29
ISO Class 6
1 000 000
237 000
102 000
35 200
8 320
293
ISO Class 7 352 000
83 200
2 930
ISO Class 8
3 520 000
832 000
29 300
ISO Class 9
35 200 000
8 320 000
293 000
ISO Class 5 Definition
Airborne particulate cleanliness inside any PCR Enclosure is designated by ISO
Class 5, which is equivalent to 3520 particles 0.5 µm or larger per cubic meter of
air per ISO Standard 14644-1. ISO Class 5 cleanliness is illustrated in the table to
follow and is equivalent to Class 100 air conditions as defined by Federal Standard
209E. Class 100 is equal to 100 particles 0.5 µm or larger per cubic foot of air.
Table 1-1 ISO Classification Number (N)
Polymerase Chain Reaction (PCR)
Definition
Polymerase Chain Reaction (PCR), is a laboratory process in which a particular
DNA segment from a mixture of DNA chains is rapidly replicated, producing a
large, readily analyzed sample of a piece of DNA. In PCR, DNA is immersed in a
solution containing the enzyme DNA polymerase, unattached nucleotide bases (the
subunits that DNA is composed of), and “primers”, short sequences of nucleotides
designed to bind with an end of the desired DNA segment. Two primers are used:
one primer binds at one end of the desired segment on one of the two paired DNA
strands and the other primer binds at the other end but on the other strand. The
solution is heated to break the bonds between the strands of the DNA. When the
solution cools, the primers bind to the separated strands, and DNA polymerase
quickly builds a new strand by joining the free nucleotide bases to the primers.
When this process is repeated, a strand that was formed with one primer binds to
the other primer, resulting in a new strand that is restricted solely to the desired
segment. Thus, the region of DNA between the primers is selectively replicated.
Further repetitions of the process can produce billions of copies of a small piece of
DNA in several hours. PCR was developed in 1985 by Kary B. Mullis, who was
awarded the 1993 Nobel Prize in chemistry for his work. It is used in a broad
range of applications from DNA fingerprinting to medical tests to identify diseases
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Chapter 1: Introduction
3
!
from the infectious agent’s DNA. See also nucleic acid. The Concise Columbia
Encyclopedia is licensed from Columbia University Press. Copyright © 1995 by
Columbia University Press. All rights reserved.
About This Manual
This manual is designed to help you learn how to install, use, and maintain your
Filtered (PCR) Enclosure.
If the unit is not operated as specified in this manual it may
impair the protection provided by the unit.
Si l'unité n'est pas utilisée comme spécifié dans ce manuel il
peut diminuer la protection fournie par l'unité.
Chapter 1: Introduction provides a brief overview of the Filtered PCR Enclosure,
explains the organization of the manual, and defines the typographical conventions
used in the manual.
Chapter 2: Prerequisites explains what you need to do to prepare your site before
you install the Filtered PCR Enclosure. Electrical and service requirements are
discussed.
Chapter 3: Getting Started contains the information you need to properly unpack,
inspect, install, and certify the Filtered PCR Enclosure.
Chapter 4: Performance Features and Safety Precautions explains how the
Purifier Filtered PCR Enclosure operates and the appropriate precautions you
should take when using it.
Chapter 5: Using Your Filtered Enclosure discusses the basic operation of how to
prepare, use and shut down your Filtered PCR Enclosure.
Chapter 6: Maintaining Your Filtered PCR Enclosure explains how to perform
routine maintenance on the PCR Enclosure.
Chapter 7: Accessorizing Your Filtered PCR Enclosure explains acceptable
modifications to the PCR Enclosure or how to add accessories.
Chapter 8: Troubleshooting contains a table of problems you may encounter while
using the Filtered PCR Enclosure including probable causes and suggested
corrective actions.
Appendix A: Components and Replacement Parts contains labeled diagrams of all
of the components of the Filtered PCR Enclosure.
Appendix B: Dimensions contains comprehensive diagrams showing all of the
dimensions for the Filtered PCR Enclosure.
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Chapter 1: Introduction
4
!
2'
3'
4'
!
Appendix C: Filtered PCR Enclosure Specifications contains the electrical
requirements for Filtered PCR Enclosures. Wiring diagrams are also included.
Appendix D: References lists the various resources available addressing clean work
stations and PCR Enclosures.
Typographical Conventions
Recognizing the following typographical conventions will help you understand and
use this manual:
Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:
Getting Started).
Steps required to perform a task are presented in a numbered format.
Comments located in the margins provide suggestions, reminders, and
references.
Critical information is presented in boldface type in paragraphs that are
preceded by the exclamation icon. Failure to comply with the information
following an exclamation icon may result in injury to the user or permanent
damage to the enclosure.
Critical information is presented in boldface type in paragraphs that are
preceded by the wrench icon. Only a trained certifier or contractor should only
perform these operations. Failure to comply with the information following a
wrench icon may result in injury to the user or permanent damage to your PCR
Enclosure.
Important information is presented in capitalized type in paragraphs that are
preceded by the pointer icon. It is imperative that the information contained in
these paragraphs be thoroughly read and understood by the user.
A number icon precedes information that is specific to a particular model of
enclosure. The 2' icon indicates the text is specific to the 2-foot wide model.
The 3' icon indicates the text is specific to the 3-foot model, etc.
CAUTION – See Manual. When this symbol is on the unit it indicates a
caution that is detailed in this manual.
ATTENTION - Voir manuel. Lorsque ce symbole est sur l'unité, il indique une
mise en garde qui est indiqué dans ce manuel.
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5
CHAPTER 2
PREREQUISITES
Before you install the Filtered PCR Enclosure, you need to prepare your site for
installation. You must be certain that the area is level and of solid construction. In
addition, a dedicated source of electrical power should be located near the
installation site to power the Filtered PCR Enclosure, and other apparatus.
Additionally, the enclosure should be strategically placed in the lab to provide
efficient workflow.
Carefully read this chapter to learn the requirements for your installation site:
The support, vibration and movement requirements.
The location and air current requirements.
The exhaust and blower requirements.
The electrical power requirements.
The space requirements.
Refer to Appendix B: Dimensions for complete enclosure dimensions.
Refer to Appendix C: Filtered PCR Enclosure Specifications for complete filtered
enclosure electrical and environmental conditions, specifications and requirements.
Support, Vibration and Movement
Requirements
At a minimum, the supporting structure usually consists of a base cabinet or base
stand and chemically-resistant work surface.
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Chapter 2: Prerequisites
6
Enclosure
Width
Model Description
Downflow
Velocity
(fpm)
Out-
Flow
Exhaust
Volume
(CFM)
Noise
Pressure
db(A)
Average
Outflow
Face
Velocity
(fpm)
2'
2' Filtered PCR Enclosure
30
40
50
60
120
165
210
250
53-57
58-60
60-61
62-63
85
115
145
170
3'
3' Filtered PCR Enclosure
30
40
50
60
180
250
310
370
58-60
60-62
62-66
66-70
85
115
145
170
4'
4' Filtered PCR Enclosure
30
40
50
60
240
330
420
500
58-62
60-64
62-66
66-70
85
115
145
170
!
Location and Air Current Requirements
The Purifier Filtered PCR Enclosures have been designed to provide particulate
free air meeting ISO Class 5 conditions by negating typical cross drafts and
turbulence within the opening. However, as a precautionary safety measure and a
higher level of quality management, it is recommended that the Filtered PCR
Enclosure be placed in an area away from:
High traffic areas where walking might cause an air disturbance or be a
nuisance.
Overhead or wall HVAC diffusers, fans, radiators or other lab equipment
producing air currents.
Doorways or windows that may be opened.
Do not position the unit so that it is difficult to operate the main
disconnect device.
Ne placez pas l'appareil de sorte qu'il est difficile de faire
fonctionner le dispositif principal de déconnexion.
Airflow Specifications
The Purifier Filtered PCR Enclosures use an integral motorized impeller to draw
room air from the top and through the enclosure. This room air is then pushed
down through the HEPA filter. The enclosure is factory set to deliver 40-55 fpm
downflow velocity measured 6" below the upper diffuser. The Class 5 HEPAfiltered air flows across the work area and exits out the front and back of the
enclosure. The Filtered PCR Enclosure provides a continuous downflow of
particulate free HEPA filtered air.
Data for the outflow exhaust volume, downflow velocities and out-flow face
velocities are listed for each Filtered PCR model below.
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Chapter 2: Prerequisites
7
Electrical Requirements
Standard duplex electrical receptacles should be nearby for connecting the Filtered
PCR Enclosure, or other equipment. The enclosure includes iris pass-throughs to
allow electrical cords through the back of the enclosure without leaving a large
hole.
Space Requirements
The dimensions for all the different models are shown in Appendix B: Dimensions.
Product Service 1-800-522-7658
8
!
CHAPTER 3
GETTING STARTED
Now that the site for your Filtered PCR Enclosure is properly prepared, you are
ready to unpack, inspect, install, and validate your enclosure. Read this chapter to
learn how to:
Unpack and move the enclosure.
Set up the enclosure with the proper supporting structure and work surface.
Verify HEPA filters integrity.
Connect the electrical supply.
Set the downflow velocity with the speed control adjustment.
Validate enclosure airflow.
Seal the enclosure to the work surface.
Depending upon which model you are installing, you may need common
mechanical and electrical installation tools in addition to wrenches, ratchets,
sockets, a nut driver set, a flat-blade screwdriver, a Phillips screwdriver, and a
carpenter level to complete the instructions in the chapter.
Each enclosure model weighs between 125 to 195 lbs. each (55
to 85 kg). The shipping container allows for lifting with a
mechanical lift truck or floor jack. If you must lift the
enclosure manually, follow safe-lifting guidelines. Do not lift
by the lower air foil.
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Chapter 3: Getting Started
9
!
The United
States
Interstate
Commerce
Commission
rules require
that claims be
filed with the
delivery
carrier within
fifteen (15)
days of
delivery.
Unpacking the Enclosure
Carefully remove the shrink-wrap or carton on the enclosure and inspect it for
damage that may have occurred in transit. If damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST
FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER.
LABCONCO CORPORATION AND ITS DEALERS ARE NOT
RESPONSIBLE FOR SHIPPING DAMAGES.
Do not discard the packing material until you have checked all of the components
and tested the enclosure. We recommend that you do not remove the enclosure
from its shipping container until it is ready to be placed into its final location.
Move the unit by placing a flat, low dolly under the shipping skid, or by using a
floor jack.
Do not move the enclosure by tilting it onto a hand truck.
Installing the Filtered PCR Enclosure on a
Supporting Structure and Work Surface
Use caution when lifting or moving the enclosure.
When installing the enclosure onto a chemical-resistant work surface or benchtop,
ensure that the structure can safely support the combined weight of the enclosure
and any related equipment. The work surface should be at least as wide and deep
as the enclosure to properly support it. The front of the enclosure should be
aligned within 0.30" (8mm) of the front of the work surface. Mounting holes are
provided in the Labconco accessory work surfaces to secure the enclosure.
Work Surface Specifications
The work surface should be smooth, rigid and durable, such as a chemical-resistant
epoxy resin. The surface should be non-porous and resistant to the materials used
in conjunction with the Purifier Filtered PCR Enclosure. The work surface should
also contain a dished recessed area for containing primary spills.
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Chapter 3: Getting Started
10
Filtered PCR Enclosure Work Surface Installation
1. Level the base cabinet or stand and the work surface. See Figure 3-1.
2. Position the work surface in its intended location and with the front of the
work surface towards you. (Rear mounting holes are located close to the
rear edge.)
3. Secure the work surface to the base cabinet or stand with a structural
adhesive or silicone sealant.
4. Insert the supplied mounting screws in the four holes. Allow a minimum
of 1/8" clearance under the head of the screw for positioning the enclosure.
5. Place the enclosure on the work surface and slide the rear flange and front
air foil flanges under the mounting screw heads.
6. Tighten the four screws to complete the installation.
Figure 3-1
Filtered PCR Enclosure Installation
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Chapter 3: Getting Started
11
HEPA Filter
Appropriate Use
Filter Size
Part No.
HEPA filters are high-efficiency particulate
air filters having a particulate removable
efficiency of 99.99% for particles with a
diameter of 0.3 micron.
2'
3707900
3'
3707901
4'
3707902
!
Verifying HEPA Filter Integrity
The HEPA filter is shipped installed with the gasket on the downstream side. The
HEPA filter is leak checked at Labconco. A second leak check is recommended
before using the enclosure and at least annually thereafter. Consult your Safety
Officer and Chapter 6 for the HEPA Filter Leak Test. See Figure 4-2 for HEPA
filter location, HEPA filter gasket, and filter clamp bolts. All seams downstream of
the HEPA filter are jacketed by positive pressure. This intrinsically safe design
ensures Class 5 conditions and a particulate free environment for PCR
experiments.
Connecting the Electrical Supply Source to the
Filtered PCR Enclosure
115V Models
Simply connect the 115V power cord supplied to the IEC electrical supply plug on
the back of the enclosure. If using at 50 Hz operations, blower performance
maximum airflow will be reduced by 17%.
230V Models
The 230V is shipped without a plug. Install the appropriate plug for your electrical
specifications per local codes.
Do not use any detachable power cord that is not adequately rated
for the unit.
Ne pas utliser un fil électrique amovible qui n’est pas du tension
nominale de l’appareil.
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Chapter 3: Getting Started
12
4'
2'
3'
Setting the Downflow Velocity with the
Speed Control
Adjustment of the speed control gives the correct downflow velocity and is located
behind the front panel. The downflow velocity should be from 30-65 fpm for
Class 100 (ISO Class 5) conditions. ISO Class 5 clean air conditions are
maximized at a setting within this range. The Filtered PCR Enclosure is factory
set at 40-55 fpm downflow velocity. (Consult your Safety Officer for airflow
recommendations for your application). Working at the lowest downflow
velocity appropriate for the application will give the quietest operation. Downflow
velocity measurements are made using an anemometer. An electric anemometer
can be obtained from your laboratory supply dealer. Downflow velocity
measurements should be taken 6" below the interior upper diffuser. Using a small
Phillips screwdriver, adjust the speed control to give the required downflow
velocity. See Figures 6-1 and 6-2 in Chapter 6 to locate the speed control. The
downflow velocity is increased by turning the speed control counterclockwise and
clockwise to decrease downflow velocity.
Validating the Filtered PCR Enclosure
To determine the actual downflow velocity, airflow velocity readings are taken.
This should be done 6" down from the upper diffuser located inside the top of the
enclosure. The “average downflow velocity” is achieved by averaging the
readings in the downflow test grid for each model:
The downflow test grid consists of 9 test points, 3 points in 3 rows. The back row
of the grid is 6" from the rear of the work area. The middle and front rows are
6.57" apart from the back to the front. The first and last column of the downflow
grid are spaced 6" in from each sidewall with a center column spaced 5.32" away
from the first and last column.
The downflow test grid consists of 12 test points, 4 points in 3 rows. The back row
of the 6" from the rear of the work area. The middle and front rows are 6.57" apart
from the back to the front. The first and last column of the downflow grid are
spaced 6" in from each sidewall with two columns spaced 7.54" away from the
first and last column.
The downflow test grid consists of 15 test points, 5 points in 3 rows. The back row
of the grid is 6" from the rear of the work area. The middle and front rows are
6.57" apart from the back to the front. The first and last column of the downflow
grid are spaced 6" in from each sidewall with three columns spaced 8.66" away
from the first and last column.
Refer to table in Chapter 2 Refer to the exhaust specification for proper airflow
volumes and downflow velocities for your particular model. A certified technician
should complete validation.
Product Service 1-800-522-7658
Chapter 3: Getting Started
13
The Purifier Filtered PCR Enclosures have been tested at Labconco before
shipment and provide ISO Class 5 clean air conditions inside the enclosure.
Labconco also performed extensive performance testing to validate the Filtered
PCR’s for product protection under ISO Class 5 conditions. For copies of these
validation reports, contact Labconco Customer Service.
Sealing the Filtered PCR Enclosure to the
Work Surface
When the Filtered PCR Enclosure has been set in place and wired, it may be sealed
at the work surface to prevent materials from collecting under the walls. A bead of
silicone sealant is recommended to seal the Filtered PCR Enclosure to the work
surface.
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