The information contained in this manual and the accompanying products are copyrighted and all
rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic
design changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
•Purifier® Logic® Biological Safety Cabinets and PuriCare® Lab Animal Research Stations
carry a three-year warranty from date of installation or four years from date of shipment from
Labconco, whichever is sooner.
•SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date
of installation or three years from date of shipment from Labconco, whichever is sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight
carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the
delivery carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed
by various federal, state, or local regulations. All users of this equipment are required to become
familiar with any regulations that apply in the user’s area concerning the dumping of waste materials
in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held
harmless with respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #5424400, Rev. -
ECO F196
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: PREREQUISITES 4
Support, Vibration & Preventive Requirements 5
Location Requirements 5
Exhaust and Blower Requirements 5
Particle Testing 6
ISO Class 3 Definition 7
Airflow and Pressure Charts 8
Electrical Requirements 12
Space Requirements 12
CHAPTER 3: GETTING STARTED 13
Unpacking the Glove Box 14
Installing the Glove Box on a Supporting Structure &
Work Surface 14
Connecting to the Exhaust System (If Applicable) 15
Outside Exhaust Connection Diagram 16
Positive Pressure Configuration 17
Installation of Gloves to the Glove Ports 18
Connecting the Electrical Supply to the Glove Box 18
Setting Exhaust Volume with Speed Control Adjustment 19
Resetting the Calibration Program 19
Validating the Glove Box 19
Summary of Performance Testing Results 20
CHAPTER 4: PERFORMANCE FEATURES AND
SAFETY PRECAUTIONS 22
Performance Features 22
Safety Precautions 25
CHAPTER 5: USING YOUR GLOVE BOX 27
Starting the Filtered Glove Box 27
Control Panel Touchpad 28
All Program Screens 29
Main Setup Screens 30
Calibration Program Screens 31
Diagnostic Program Screens 32
Routine Daily Work Procedures 33
O
O
FF
C
C
OONNTTEENNTTSS
Appropriate HEPA or ULPA Filter Applications,
Suitability & Guidelines 34
Definition of Terms 34
Appropriate Chemicals for Carbon Filters 35
Prohibited Acid Use 36
CHAPTER 6: MAINTAINING YOUR GLOVE BOX 37
Routine Maintenance Schedule 38
Decontamination 38
Determining When to Replace Filters 39
How to Install New Filters 39
HEPA or ULPA Filter Leak Test 41
Initial Certification 43
Re-Certification 43
Fluorescent Light Replacement 43
Motorized Impeller Replacement 45
Speed Control Replacement 46
Airflow/Pressure Monitor Circuit Board Replacement 46
CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 48
CHAPTER 8: TROUBLESHOOTING 54
APPENDIX A: REPLACEMENT PARTS 55
APPENDIX B: DIMENSIONS 58
APPENDIX C: SPECIFICATIONS 59
APPENDIX D: IONIZER FAN PERFORMANCE CRITERIA 60
APPENDIX E: CONVERSIONS 62
APPENDIX F: REFERENCES 63
DECLARATION OF CONFORMITY 64
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco Protector® Filtered Glove
Box. The stainless steel-lined glove box uses ULPA filters and the fiberglass
glove box uses HEPA filters. Your glove box provides personnel protection
through superior containment.
These glove boxes effectively contain toxic or noxious particulates when
properly installed and operated. In standard negative or optional positive
pressure operation modes, the glove box can create an ISO standard Class 3
(ISO 14644-1) clean air environment. This glove box uses either HEPA
filters, rated at least 99.99% efficient for 0.3-micron particles or ULPA
filters, rated at least 99.999% efficient for 0.12-micron particles.
The Protector Filtered Glove Boxes offer many unique features to enhance
safety and performance. To take full advantage of them, please acquaint
yourself with this manual and keep it handy for future reference. All glove
box operators must read and understand how filtered glove boxes operate;
please review Chapter 4: Performance Features and Safety Precautions
before beginning to work with this glove box. Even if you are an
experienced user, please review Chapter 5: Using Your Filtered Glove Box,
which describes the Protector features so that you can use the filtered glove
box effectively and safely.
Product Service 1-800-522-7658
1
Chapter 1: Introduction
Protector Stainless
Steel ULPA-Filtered
Glove Box shown on
Adjustable Height
Base Stand.
2
Figure 1-1
Product Service 1-800-522-7658
Chapter 1: Introduction
Protector Double
Width Stainless Steel
ULPA-Filtered Glove
Box shown on
Doublewide Base
Stand.
Figure 1-2
Product Service 1-800-522-7658
3
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install the glove box, you need to prepare your site for
installation. You must be certain that the area is level and of solid
construction. In addition, a dedicated source of electrical power should be
located near the installation site to power the glove box. The glove box
should be strategically placed in the lab to provide efficient workflow and
prevent operator interference from normal traffic patterns.
Carefully read this chapter to learn the requirements for your installation site:
• The support, vibration and preventive requirements.
• The location requirements.
• The exhaust and blower requirements.
• The electrical power requirements.
• The space requirements.
Refer to Appendix B: Protector Filtered Glove Box Dimensions for complete
glove box dimensions.
Refer to Appendix C:Protector Filtered Glove Box Specifications for
complete electrical and environmental conditions, specifications and
requirements.
4
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Support, Vibration and Preventive Requirements
In the preparation of a glove box site, please consider the following:
•A bench or stand that is rigidly mounted to the floor or fixed to the wall,
but not both, may be appropriate. 35" to 40" (889mm-1016mm) is
typical for standing height. (Labconco stands offered in Chapter 7 vary
from 33" to 40".)
•The corners of a building typically have less vibration than the center,
which promotes analytical balance stability.
•The bench typically should not contain any vibration-producing
equipment, such as shakers or pumps.
•A marble slab with dampening pads placed within the enclosure is an
effective low cost means of controlling vibration (see Chapter 7: Accessorizing Your Filtered Glove Box).
Location Requirements
The Protector Filtered Glove Boxes have been designed to rest on a typical
29"-30" (737mm-762mm) deep work surface. The height should be 35"-40"
for standing position. Avoid placing the glove box in high traffic areas where
walking might disrupt the operator or experimentation.
Exhaust and Blower Requirements
The Protector Filtered Glove Box, as manufactured, uses an integral
motorized impeller to pull room air through the inlet filter and exhaust the air
through the outlet filter to discharge the clean air back into the laboratory.
This negative pressure operation mode pulls all internally-contaminated air
through the exhaust filter. The filtered exhaust air is then forced out the top
of the glove box. An optional FilterMate™ Portable Exhauster with a carbon
filter may be installed downstream of the exhaust to adsorb low levels of
organic fumes, formaldehyde, or ammonia gases.
The filtered exhaust air from the glove box can be exhausted to the outside
with the installation of a thimble exhaust connection kit and remote blower
listed in Chapter 7.
Labconco does not recommend a hard duct connection using the built-in
motorized impeller as the source of airflow out of the facility. This can
create a positive pressure in the exhaust duct and is harder to maintain.
Product Service 1-800-522-7658
5
Chapter 2: Prerequisites
Labconco does not recommend hard duct connections with remote
blowers due to the air volume balance requirements with such systems.
Hard duct connections should only be used when the glove box impeller
is disconnected and only a single roof-mounted blower is used. Note that
reliance on an external exhaust blower does not provide airflow control
at the glove box.
If a positive internal pressure clean air ISO Class 3 condition (ISO 14644-1)
is desired, the motorized impeller can be rotated to work in positive pressure
mode (see Figure 3-2). See Chapter 7 for the Positive Pressure Conversion
Kit. See the following Negative and Positive Pressure Particle Test data in
the charts below. Both charts show the fast dilution rates of the glove boxes.
500
NOTE: Ambient Air Baseline Particle Count at 1 Minute
450
132155 Particles measuring 0.3 Micron detected within 1 Minute.
6499 Particles measuring 0.5 Micron detected within 1 Minute.
(Per Cubic Meter Per Minute)
400
350
300
250
200
150
100
400
143
144
123123
Airflow Testing Performed at 42 CFM
with Glove Box Under Negative Pressure.
> 0.3 Micron
> 0.5 Micron
107
103103
Number of Particles
Protector HEPA-Filtered Glove Box Particle Testing under Negative Pressure
50
0
0
0
012345678910111213141516
38
8
6
7
9
5
8
2
Time (Minutes)
100
5
6
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Protector HEPA-Filtered Glove Box Particle Testing under Positive Pressure
500
450
400
350
300
269
(Per Cubic Meter Per Minute)
250
200
150
100
98
NOTE: Ambient Air Baseline Particle Count at 1 Minute
32151 Particles measuring 0.3 Micron detected w ithin 1 Minute.
1074 Particles measuring 0.5 Micron detected within 1 Minute.
314
115
83
Number of Particles
50
0
0
012345678910111213141516
17
4
3
ISO Class 3 Definition
56
2
Time (Minutes)
Airflow Testing Performed at 42 CFM
with Glove Box Under Positive Pressure.
> 0.3 Micron
> 0.5 Micron
23
12
00
25
22
13
6
00
Airborne particulate cleanliness inside any clean air glove box or enclosure is
designated by ISO Class 3, which is equivalent to 35 particles 0.5 µm or
larger per cubic meter of air per minute as defined by ISO Standard 14644-1.
ISO Class 3 cleanliness is illustrated in the table below and is equivalent to
Class 1 air conditions as defined by Federal Standard 209E or l particle 0.5
µm or larger per cubic foot of air per minute.
Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.
ISO
classification
number (N)
ISO Class 1 10 2
ISO Class 2 100 24 10 4
ISO Class 3 1 000 237 102 35 8
ISO Class 4 10 000 2 370 1 020 352 83
ISO Class 5 100 000 23 700 10 200 3 520 832 29
ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293
ISO Class 7 352 000 83 200 2 930
ISO Class 8 3 520 000 832 000 29 300
Maximum concentration limits (particles/m3 of air/minute) for particles equal to
and larger than the considered sizes shown below (concentration limits are
calculated in accordance with 3.2 of Standard 14644-1)
0.1 µm 0.2 µm 0.3 µm 0.5 µm 1 µm 5 µm
ISO Class 9 35 200 000 8 320 000 293 000
Table 2-1 ISO Classification Number (N)
Product Service 1-800-522-7658
7
% Blower Speed
Control Setting
Min. 18%
35%
55%
75%
Max. 100%
Dial OFF
Chapter 2: Prerequisites
Additionally, the motorized impeller can also be used in a full recirculation
mode providing the operator with some control of the atmosphere chemistry
within the glove box. See Chapter 7 for the Recirculation Conversion Kit.
The airflow charts below represents typical operational data including air
changes per minute, exhaust volume, noise levels, main chamber static
pressures, and total system static pressures measured at the blower intake
(suction side). Exhaust volumes are reduced by 17% for products operating
at 230V. Exhaust volumes are reduced by 22% for ULPA filters. Exhaust
volumes are reduced by 17% for products operating on 100V, 60 Hz and
reduced 34% for 100V, 50 Hz products.
Air Changes
per Minute
(ACM)
0.9
1.8
2.8
3.8
5.0
0
HEPA Exhaust
Volume
(CFM)
15
30
48
65
85
0
Noise Pressure
dB (A)
52
54
59
63
68
37
Static Pressure
Inside Glove Box
(inches of water)
0.12
0.30
0.54
0.87
1.28
0
Total System
Static Pressure
(inches of water)
0.31
0.62
1.14
1.65
2.48
0
PROTECTOR HEPA-FILTERED GLOVE BOX
2.5
Static Pressure Test Results
Static Pressure Inside Glove Box
2
1.5
1
(Inches of Water Column)
Negative Static Pressure
0.5
0
1015202530354045505560657075808590
Total Static Pressure at Blower
Intake (Suction Side)
0.72
0.57
0.31
0.12
0.40
0.19
0.27
0.36
Airflow Volume
1.65
1.39
1.12
0.87
0.68
0.52
(Cubic Feet per Minute)
1.94
1.02
2.48
1.28
Static Pressure test results on the Protector HEPA-Filtered Glove Box
Note: Test results are approximately the same for positive pressure application
Total Static Pressure at Blower
Intake (Suction Side)
1.5
1.23
1
(Inches of Water Column)
Negative Static Pressure
0.5
0
202530354045505560
0.52
0.30
0.89
0.51
Airflow Volume
0.72
(Cubic Feet per Minute)
1.41
0.83
Static Pressure test results on the Protector ULPA-Filtered Glove Box
Note: Test results are approximately the same for positive pressure application
1.67
1.00
Product Service 1-800-522-7658
11
!
Chapter 2: Prerequisites
For thimble-ducted glove boxes, the proper remote blower selection can be
determined from these exhaust requirements as the remote blower only needs
to be sized for the static pressure loss of the ductwork system. The thimble
ducted exhaust blower should be sized for 25% additional airflow. When
exhausting a glove box to the outside, it should be connected to a dedicated
remote blower.
Labconco offers accessory remote blowers listed in Chapter 7. Contact
Labconco for blower sizing assistance.
When a glove box is connected to a remote blower exhaust system, an
adjustable damper (or valve) should be installed in the duct to control the
airflow volumes properly. See Chapter 7 for Exhaust Dampers.
Electrical Requirements
Electrical service should be nearby for connecting the glove box cords, and
accessory equipment. Single width units require one line cord and double width
units require two line cords. Line cords are each rated at 100-115V, 15A or
230V, 12A. The glove box is rated 115V, 12A or 230V, 12A or 100V, 12A. A
convenience outlet is located inside the glove box and is rated for 115V, 12A or
230V, 6A or 100V, 12A. Additional power strip accessories are available for
connection to the outlet and are listed in Chapter 7.
Space Requirements
The dimensions are shown in Appendix B: Dimensions. Adequate space to
the right of the transfer chamber is required for loading and unloading.
12
Product Service 1-800-522-7658
CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Once the site for your Protector Glove Box is properly prepared, you are
ready to unpack, inspect, install, and validate your system. Read this chapter
to learn how to:
• Unpack and move the glove box.
• Set up the glove box with the proper supporting structure and work
surface.
• Connect to an exhaust system if applicable.
• Install the gloves.
• Connect the electrical supply.
• Set the exhaust volume with the speed control adjustment.
• Reset the calibration program if applicable.
• Arrange validation for the glove box.
Each Protector Glove Box weighs 350 lbs. (158 kg) for
!
fiberglass models, 500 lbs. (225 kg) for single width stainless
steel models, and 1000 lbs. (450 kg) for double width
stainless steel models. The shipping container allows for
lifting with a mechanical lift truck or floor jack. If you must
lift the enclosure manually, follow safe-lifting guidelines. Do
not lift by the upper sheet metal façade and front panel as
damage can occur.
Product Service 1-800-522-7658
13
Chapter 3: Getting Started
Unpacking the Glove Box
We recommend that you do not remove the glove box from its shipping
container until it is ready to be placed into its final location. Move the unit
by placing a flat, low dolly under the shipping skid, or by using a floor jack.
Carefully remove the shrink-wrap and crating on the glove box and inspect it
for damage that may have occurred in transit. If damaged, notify the
delivery carrier immediately and retain the entire shipment intact for
inspection by the carrier.
THE UNITED STATES INTERSTATE COMMERCE
COMMISSION RULES REQUIRE THAT CLAIMS BE
)
)
)
FILED WITH THE DELIVERY CARRIER WITHIN
FIFTEEN (15) DAYS OF DELIVERY.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU
MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT
CARRIER. LABCONCO CORPORATION AND ITS
DEALERS ARE NOT RESPONSIBLE FOR SHIPPING
DAMAGES.
Do not discard the packing material until you have checked all of the
components and tested the glove box.
Installing the Glove Box on a Supporting
Structure and Work Surface
Exercise caution when lifting or moving the glove box.
When installing the glove box onto a work surface or benchtop, ensure that
the structure can safely support the combined weight of the glove box and
any related equipment. The work surface should be as wide as the glove box
to properly support it. The front of the glove box should be aligned with the
front of the work surface for optimal comfort. A height of 35"-40" (889mm1016mm) is appropriate for standing operation. Adjustable Height Base
Stands listed in Chapter 7 may be adjusted from 33"-40" (838mm-1016mm).
14
Product Service 1-800-522-7658
Chapter 3: Getting Started
Connecting to the Exhaust System (If Applicable)
WARNING: The weight of any exhaust ductwork system
!
The Protector Filtered Glove Box has been manufactured to exhaust into the
laboratory in its standard configuration. To remove chemical fumes and
odors not trapped by the HEPA or ULPA filter, the glove box can be
exhausted to the outside (see Figure 3-1), or connected to an accessory
FilterMate Portable Exhauster with carbon filters. See Chapter 7 for
accessories including: Thimble Exhaust Connection, FilterMate Portable
Exhauster and separate Exhaust Kit, Positive Pressure Conversion Kit,
Recirculation Kit, or Hard Duct Connection Kit.
Consult Labconco Customer Service if you require help sizing a remote
blower for the exhaust volume and the duct work system (static pressure loss
listed in Chapter 2).
!
must be supported independently of the glove box
superstructure or damage may occur.
The exhaust system should be installed by a qualified
HVAC contractor.
To ensure compatibility, the selected exhaust duct material
should match the enclosure, procedures and chemical
applications.
Product Service 1-800-522-7658
15
Chapter 3: Getting Started
16
Figure 3-1
6" Thimble Exhaust Connection to Outside
(all dimension in inches)
Product Service 1-800-522-7658
Chapter 3: Getting Started
Positive Pressure Glove Box Configuration
(shown with a portion of the front panel removed)
Product Service 1-800-522-7658
Figure 3-2
17
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