Labconco Protector Filtered Glove Box User Manual

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User’s Manual

Protector®Filtered Glove Boxes

Electrical Receptacle Voltage/Frequency

Base

Catalog

Model No.

50650 Fiberglass HEPA Single -10 -31 -35 -40 -45 50655 Stainless

50655 Stainless

Liner

Material

Steel

Filter Type

ULPA Single -10 -31 -35 -40 -45

ULPA Double -12 -33 -37 -42 -47

Liner

Width

North

America

100-115V

50/60 Hz

British

(UK) 230V

50/60 Hz

Schuko

230V

50/60 Hz

China/

Australia

230V

50/60 Hz

60 Hz only

North

America

230V

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Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com

Please read the User’s Manual before operating the equipment.

The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of defective material and workmanship, provided such defect results from normal and proper use of the equipment.

The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner, except the following;

• Purifier® Logic® Biological Safety Cabinets and PuriCare® Lab Animal Research Stations

carry a three-year warranty from date of installation or four years from date of shipment from Labconco, whichever is sooner.

• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date

of installation or three years from date of shipment from Labconco, whichever is sooner.

• Blood Drawing Chairs carry a ten year warranty.

• Carts carry a lifetime warranty.

• Glassware is not warranted from breakage when dropped or mishandled.

This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This statement may be altered by a specific published amendment. No individual has authorization to alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or accidental breakage is not covered.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.

Part #5424400, Rev. -

ECO F196

AABBLLEE

CHAPTER 1: INTRODUCTION 1

CHAPTER 2: PREREQUISITES 4

Support, Vibration & Preventive Requirements 5 Location Requirements 5 Exhaust and Blower Requirements 5 Particle Testing 6 ISO Class 3 Definition 7 Airflow and Pressure Charts 8 Electrical Requirements 12 Space Requirements 12

CHAPTER 3: GETTING STARTED 13 Unpacking the Glove Box 14 Installing the Glove Box on a Supporting Structure &

Work Surface 14 Connecting to the Exhaust System (If Applicable) 15 Outside Exhaust Connection Diagram 16 Positive Pressure Configuration 17 Installation of Gloves to the Glove Ports 18 Connecting the Electrical Supply to the Glove Box 18 Setting Exhaust Volume with Speed Control Adjustment 19 Resetting the Calibration Program 19 Validating the Glove Box 19 Summary of Performance Testing Results 20

CHAPTER 4: PERFORMANCE FEATURES AND SAFETY PRECAUTIONS 22

Performance Features 22 Safety Precautions 25

CHAPTER 5: USING YOUR GLOVE BOX 27 Starting the Filtered Glove Box 27 Control Panel Touchpad 28 All Program Screens 29 Main Setup Screens 30 Calibration Program Screens 31 Diagnostic Program Screens 32 Routine Daily Work Procedures 33

OONNTTEENNTTSS

Appropriate HEPA or ULPA Filter Applications,

Suitability & Guidelines 34 Definition of Terms 34 Appropriate Chemicals for Carbon Filters 35 Prohibited Acid Use 36

CHAPTER 6: MAINTAINING YOUR GLOVE BOX 37 Routine Maintenance Schedule 38 Decontamination 38 Determining When to Replace Filters 39 How to Install New Filters 39 HEPA or ULPA Filter Leak Test 41 Initial Certification 43 Re-Certification 43 Fluorescent Light Replacement 43 Motorized Impeller Replacement 45 Speed Control Replacement 46 Airflow/Pressure Monitor Circuit Board Replacement 46

CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 48

CHAPTER 8: TROUBLESHOOTING 54

APPENDIX A: REPLACEMENT PARTS 55

APPENDIX B: DIMENSIONS 58

APPENDIX C: SPECIFICATIONS 59

APPENDIX D: IONIZER FAN PERFORMANCE CRITERIA 60

APPENDIX E: CONVERSIONS 62

APPENDIX F: REFERENCES 63

DECLARATION OF CONFORMITY 64

CChhaapptteerr 11:: IInnttrroodduuccttiioonn

Congratulations on your purchase of a Labconco Protector® Filtered Glove Box. The stainless steel-lined glove box uses ULPA filters and the fiberglass glove box uses HEPA filters. Your glove box provides personnel protection through superior containment.

These glove boxes effectively contain toxic or noxious particulates when properly installed and operated. In standard negative or optional positive pressure operation modes, the glove box can create an ISO standard Class 3 (ISO 14644-1) clean air environment. This glove box uses either HEPA filters, rated at least 99.99% efficient for 0.3-micron particles or ULPA filters, rated at least 99.999% efficient for 0.12-micron particles.

The Protector Filtered Glove Boxes offer many unique features to enhance safety and performance. To take full advantage of them, please acquaint yourself with this manual and keep it handy for future reference. All glove box operators must read and understand how filtered glove boxes operate; please review Chapter 4: Performance Features and Safety Precautions before beginning to work with this glove box. Even if you are an experienced user, please review Chapter 5: Using Your Filtered Glove Box, which describes the Protector features so that you can use the filtered glove box effectively and safely.

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Chapter 1: Introduction

Protector Stainless Steel ULPA-Filtered Glove Box shown on Adjustable Height Base Stand.

Figure 1-1

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Chapter 1: Introduction

Protector Double Width Stainless Steel ULPA-Filtered Glove Box shown on Doublewide Base Stand.

Figure 1-2

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CChhaapptteerr 22:: PPrreerreeqquuiissiitteess

Before you install the glove box, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the glove box. The glove box should be strategically placed in the lab to provide efficient workflow and prevent operator interference from normal traffic patterns.

Carefully read this chapter to learn the requirements for your installation site:

• The support, vibration and preventive requirements.

• The location requirements.

• The exhaust and blower requirements.

• The electrical power requirements.

• The space requirements.

Refer to Appendix B: Protector Filtered Glove Box Dimensions for complete glove box dimensions.

Refer to Appendix C: Protector Filtered Glove Box Specifications for complete electrical and environmental conditions, specifications and requirements.

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Chapter 2: Prerequisites

Support, Vibration and Preventive Requirements

In the preparation of a glove box site, please consider the following:

• A bench or stand that is rigidly mounted to the floor or fixed to the wall,

but not both, may be appropriate. 35" to 40" (889mm-1016mm) is typical for standing height. (Labconco stands offered in Chapter 7 vary from 33" to 40".)

• The corners of a building typically have less vibration than the center,

which promotes analytical balance stability.

• The bench typically should not contain any vibration-producing

equipment, such as shakers or pumps.

• A marble slab with dampening pads placed within the enclosure is an

effective low cost means of controlling vibration (see Chapter 7: Accessorizing Your Filtered Glove Box).

Location Requirements

The Protector Filtered Glove Boxes have been designed to rest on a typical 29"-30" (737mm-762mm) deep work surface. The height should be 35"-40" for standing position. Avoid placing the glove box in high traffic areas where walking might disrupt the operator or experimentation.

Exhaust and Blower Requirements

The Protector Filtered Glove Box, as manufactured, uses an integral motorized impeller to pull room air through the inlet filter and exhaust the air through the outlet filter to discharge the clean air back into the laboratory. This negative pressure operation mode pulls all internally-contaminated air through the exhaust filter. The filtered exhaust air is then forced out the top of the glove box. An optional FilterMate™ Portable Exhauster with a carbon filter may be installed downstream of the exhaust to adsorb low levels of organic fumes, formaldehyde, or ammonia gases.

The filtered exhaust air from the glove box can be exhausted to the outside with the installation of a thimble exhaust connection kit and remote blower listed in Chapter 7.

Labconco does not recommend a hard duct connection using the built-in motorized impeller as the source of airflow out of the facility. This can create a positive pressure in the exhaust duct and is harder to maintain.

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Chapter 2: Prerequisites

Labconco does not recommend hard duct connections with remote blowers due to the air volume balance requirements with such systems. Hard duct connections should only be used when the glove box impeller is disconnected and only a single roof-mounted blower is used. Note that reliance on an external exhaust blower does not provide airflow control at the glove box.

If a positive internal pressure clean air ISO Class 3 condition (ISO 14644-1) is desired, the motorized impeller can be rotated to work in positive pressure mode (see Figure 3-2). See Chapter 7 for the Positive Pressure Conversion Kit. See the following Negative and Positive Pressure Particle Test data in the charts below. Both charts show the fast dilution rates of the glove boxes.

500

NOTE: Ambient Air Baseline Particle Count at 1 Minute

450

132155 Particles measuring 0.3 Micron detected within 1 Minute. 6499 Particles measuring 0.5 Micron detected within 1 Minute.

(Per Cubic Meter Per Minute)

400

350

300

250

200

150

100

400

143

144

123 123

Airflow Testing Performed at 42 CFM

with Glove Box Under Negative Pressure.

> 0.3 Micron

> 0.5 Micron

107

103 103

Number of Particles

Protector HEPA-Filtered Glove Box Particle Testing under Negative Pressure

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Time (Minutes)

100

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Chapter 2: Prerequisites

Protector HEPA-Filtered Glove Box Particle Testing under Positive Pressure

500

450

400

350

300

269

(Per Cubic Meter Per Minute)

250

200

150

100

NOTE: Ambient Air Baseline Particle Count at 1 Minute 32151 Particles measuring 0.3 Micron detected w ithin 1 Minute. 1074 Particles measuring 0.5 Micron detected within 1 Minute.

314

115

Number of Particles

012345678910111213141516

ISO Class 3 Definition

Time (Minutes)

Airflow Testing Performed at 42 CFM

with Glove Box Under Positive Pressure.

> 0.3 Micron

> 0.5 Micron

2 2

Airborne particulate cleanliness inside any clean air glove box or enclosure is designated by ISO Class 3, which is equivalent to 35 particles 0.5 µm or larger per cubic meter of air per minute as defined by ISO Standard 14644-1. ISO Class 3 cleanliness is illustrated in the table below and is equivalent to Class 1 air conditions as defined by Federal Standard 209E or l particle 0.5 µm or larger per cubic foot of air per minute.

Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.

ISO

classification

number (N)

ISO Class 1 10 2

ISO Class 2 100 24 10 4

ISO Class 3 1 000 237 102 35 8

ISO Class 4 10 000 2 370 1 020 352 83

ISO Class 5 100 000 23 700 10 200 3 520 832 29

ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293

ISO Class 7 352 000 83 200 2 930

ISO Class 8 3 520 000 832 000 29 300

Maximum concentration limits (particles/m3 of air/minute) for particles equal to and larger than the considered sizes shown below (concentration limits are calculated in accordance with 3.2 of Standard 14644-1)

0.1 µm 0.2 µm 0.3 µm 0.5 µm 1 µm 5 µm

ISO Class 9 35 200 000 8 320 000 293 000

Table 2-1 ISO Classification Number (N)

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% Blower Speed

Control Setting

Min. 18%

35% 55% 75%

Max. 100%

Dial OFF

Chapter 2: Prerequisites

Additionally, the motorized impeller can also be used in a full recirculation mode providing the operator with some control of the atmosphere chemistry within the glove box. See Chapter 7 for the Recirculation Conversion Kit.

The airflow charts below represents typical operational data including air changes per minute, exhaust volume, noise levels, main chamber static pressures, and total system static pressures measured at the blower intake (suction side). Exhaust volumes are reduced by 17% for products operating at 230V. Exhaust volumes are reduced by 22% for ULPA filters. Exhaust volumes are reduced by 17% for products operating on 100V, 60 Hz and reduced 34% for 100V, 50 Hz products.

Air Changes

per Minute

(ACM)

0.9

1.8

2.8

3.8

5.0 0

HEPA Exhaust

Volume

(CFM)

15 30 48 65 85

Noise Pressure

dB (A)

52 54 59 63 68 37

Static Pressure

Inside Glove Box

(inches of water)

0.12

0.30

0.54

0.87

1.28 0

Total System

Static Pressure

(inches of water)

0.31

0.62

1.14

1.65

2.48 0

PROTECTOR HEPA-FILTERED GLOVE BOX

2.5

Static Pressure Test Results

Static Pressure Inside Glove Box

1.5

(Inches of Water Column)

Negative Static Pressure

0.5

10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90

Total Static Pressure at Blower Intake (Suction Side)

0.72

0.57

0.31

0.12

0.40

0.19

0.27

0.36

Airflow Volume

1.65

1.39

1.12

0.87

0.68

0.52

(Cubic Feet per Minute)

1.94

1.02

2.48

1.28

Static Pressure test results on the Protector HEPA-Filtered Glove Box

Note: Test results are approximately the same for positive pressure application

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Chapter 2: Prerequisites

PROTECTOR STAINLESS STEEL HEPA-FILTERED GLOVE BOX (230V)

PROTECTOR HEPA-FILTERED GLOVE BOX (230V)

Static Pressure Test Results

2.5

Static Pressure Inside Glove Box

Total Static Pressure at Blower Intake (Suction Side)

1.5

1.09

(Inches of Water Column)

Negative Static Pressure

0.5

0.44

0.26

20 25 30 35 40 45 50 55 60 65 70 75

0.54

0.32

Airflow Volume

0.81

0.68

0.49

(Cubic Feet per Minute)

1.45

0.93

Static Pressure test results on the Protector HEPA-Filtered Glove Box Note: Test results are approximately the same for positive pressure application

1.62

1.03

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Chapter 2: Prerequisites

PROTECTOR ULPA-FILTERED GLOVE BOX (115V)

2.5

Static Pressure Inside Glove Box

Static Pressure Test Results

2.41

Total Static Pressure at Blower

1.91

Intake (Suction Side)

1.60

1.5

Negative Static Pressure

1.13

(Inches of Water Column)

0.5

0.44

0.23

10 15 20 25 30 35 40 45 50 55 60 65 70

0.75

0.62

0.40

Airflow Volume

0.90

(Cubic Feet per Minute)

1.08

Static Pressure test results on the Protector ULPA-Filtered Glove Box

Note: Test results are approximately the same for positive pressure application.

1.36

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Chapter 2: Prerequisites

PROTECTOR STAINLESS STEEL ULPA-FILTERED GLOVE BOX (230V)

PROTECTOR ULPA-FILTERED GLOVE BOX (230V)

Static Pressure Test Results

2.5

Static Pressure Inside Glove Box

Total Static Pressure at Blower Intake (Suction Side)

1.5

1.23

(Inches of Water Column)

Negative Static Pressure

0.5

20 25 30 35 40 45 50 55 60

0.52

0.30

0.89

0.51

Airflow Volume

0.72

(Cubic Feet per Minute)

1.41

0.83

Static Pressure test results on the Protector ULPA-Filtered Glove Box

Note: Test results are approximately the same for positive pressure application

1.67

1.00

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Chapter 2: Prerequisites

For thimble-ducted glove boxes, the proper remote blower selection can be determined from these exhaust requirements as the remote blower only needs to be sized for the static pressure loss of the ductwork system. The thimble ducted exhaust blower should be sized for 25% additional airflow. When exhausting a glove box to the outside, it should be connected to a dedicated remote blower.

Labconco offers accessory remote blowers listed in Chapter 7. Contact Labconco for blower sizing assistance.

When a glove box is connected to a remote blower exhaust system, an adjustable damper (or valve) should be installed in the duct to control the airflow volumes properly. See Chapter 7 for Exhaust Dampers.

Electrical Requirements

Electrical service should be nearby for connecting the glove box cords, and accessory equipment. Single width units require one line cord and double width units require two line cords. Line cords are each rated at 100-115V, 15A or 230V, 12A. The glove box is rated 115V, 12A or 230V, 12A or 100V, 12A. A convenience outlet is located inside the glove box and is rated for 115V, 12A or 230V, 6A or 100V, 12A. Additional power strip accessories are available for connection to the outlet and are listed in Chapter 7.

Space Requirements

The dimensions are shown in Appendix B: Dimensions. Adequate space to the right of the transfer chamber is required for loading and unloading.

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CChhaapptteerr 33:: GGeettttiinngg SSttaarrtteedd

Once the site for your Protector Glove Box is properly prepared, you are ready to unpack, inspect, install, and validate your system. Read this chapter to learn how to:

• Unpack and move the glove box.

• Set up the glove box with the proper supporting structure and work

surface.

• Connect to an exhaust system if applicable.

• Install the gloves.

• Connect the electrical supply.

• Set the exhaust volume with the speed control adjustment.

• Reset the calibration program if applicable.

• Arrange validation for the glove box.

Each Protector Glove Box weighs 350 lbs. (158 kg) for

fiberglass models, 500 lbs. (225 kg) for single width stainless steel models, and 1000 lbs. (450 kg) for double width stainless steel models. The shipping container allows for lifting with a mechanical lift truck or floor jack. If you must lift the enclosure manually, follow safe-lifting guidelines. Do not lift by the upper sheet metal façade and front panel as damage can occur.

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Chapter 3: Getting Started

Unpacking the Glove Box

We recommend that you do not remove the glove box from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack.

Carefully remove the shrink-wrap and crating on the glove box and inspect it for damage that may have occurred in transit. If damaged, notify the

delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.

THE UNITED STATES INTERSTATE COMMERCE COMMISSION RULES REQUIRE THAT CLAIMS BE

)

FILED WITH THE DELIVERY CARRIER WITHIN FIFTEEN (15) DAYS OF DELIVERY.

DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.

IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.

Do not discard the packing material until you have checked all of the components and tested the glove box.

Installing the Glove Box on a Supporting Structure and Work Surface

Exercise caution when lifting or moving the glove box.

When installing the glove box onto a work surface or benchtop, ensure that the structure can safely support the combined weight of the glove box and any related equipment. The work surface should be as wide as the glove box to properly support it. The front of the glove box should be aligned with the front of the work surface for optimal comfort. A height of 35"-40" (889mm1016mm) is appropriate for standing operation. Adjustable Height Base Stands listed in Chapter 7 may be adjusted from 33"-40" (838mm-1016mm).

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Chapter 3: Getting Started

Connecting to the Exhaust System (If Applicable)

WARNING: The weight of any exhaust ductwork system

The Protector Filtered Glove Box has been manufactured to exhaust into the laboratory in its standard configuration. To remove chemical fumes and odors not trapped by the HEPA or ULPA filter, the glove box can be exhausted to the outside (see Figure 3-1), or connected to an accessory FilterMate Portable Exhauster with carbon filters. See Chapter 7 for accessories including: Thimble Exhaust Connection, FilterMate Portable Exhauster and separate Exhaust Kit, Positive Pressure Conversion Kit, Recirculation Kit, or Hard Duct Connection Kit.

Consult Labconco Customer Service if you require help sizing a remote blower for the exhaust volume and the duct work system (static pressure loss listed in Chapter 2).

must be supported independently of the glove box superstructure or damage may occur.

The exhaust system should be installed by a qualified HVAC contractor.

To ensure compatibility, the selected exhaust duct material should match the enclosure, procedures and chemical applications.

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Chapter 3: Getting Started

Figure 3-1

6" Thimble Exhaust Connection to Outside

(all dimension in inches)

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Chapter 3: Getting Started

Positive Pressure Glove Box Configuration

(shown with a portion of the front panel removed)

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Figure 3-2

Chapter 3: Getting Started

Installation of Gloves to the Glove Ports

With thumbs up and right/left orientation, secure the gloves in place on the glove ports by stretching the beaded cuff into the groove nearest the window. Install the separate 8" diameter O-ring over the gloves and into the outer groove of the glove port surface. Stainless steel band clamps are provided for securing the separate O-ring into the glove port groove. Replacement gloves and parts are listed in Appendix A with a description in Chapter 7.

Figure 3-3

Glove Installation

Connecting the Electrical Supply to the Glove Box

115V, 60 Hz; 100V, 50 Hz or 100V, 60 Hz Models

Connect the power cord supplied to the IEC electrical supply plug on the back of the glove box. Double width models utilize two power cords. 115V or 100V models are rated at 12 amps total.

The maximum circuit load for the interior electrical duplex is 10 amps.

230V, 50 Hz or 230V, 60 Hz Models

The same procedure applies for the 230V models. Install the power cord per local requirements. The 230V, 50 Hz or 230V, 60 Hz models are both rated at 12 amps total. The interior electrical duplex is rated at 10 amps.

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Chapter 3: Getting Started

Setting the Exhaust Volume with the Speed Control Adjustment

For Protector Filtered Glove Boxes, the speed control located on the front panel of the transfer chamber adjusts the exhaust air volume. The exhaust air volume should be adjusted to the lower settings for weigh operations.

(Consult your Safety Officer for airflow recommendations for your application). Working at the lowest exhaust volume appropriate for the

application will give the quietest operation. To increase the dilution rate of air volume changes after working in the glove box and to ensure proper cleaning, the speed control should be maintained at a higher setting above 3540 CFM for 5 to 10 minutes as recommended by the operator’s predetermined protocol. The exhaust volume is increased by turning the speed control counterclockwise.

Resetting the Calibration Program

With the gloves installed and transfer chamber doors closed, the maximum airflow volume (CFM) for the glove box has been preset between 50–85 CFM with a main chamber maximum pressure above 0.7" water gauge. Important Note: Transport, altitude and temperature differences may require a reset of the “Calibration Program” as outlined in Chapter 5. When “Calibration” is reset, it is best to try the previously calibrated values shown in “Diagnostics” as outlined in Chapter 5 and the test report form that shipped with the glove box.

Validating the Glove Box

The Protector Filtered Glove Box has been leak tested at the factory for filter and main chamber pressure integrity. The HEPA or ULPA filters should be leak checked upon start-up and annually thereafter per the procedure in Chapter 6. Upon start up, the main chamber pressure should be greater

than 0.7" w.g. at full blower speed to validate proper blower operation; if not, contact Labconco for service.

NOTE: Main chamber pressure and smoke removal tests

should be performed frequently, per your established quality system, to ensure safe performance.

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Chapter 3: Getting Started

Summary of Performance Testing Results

Labconco’s test results show that the Protector Filtered Glove Box meets all the performance criteria when operated at 50% airflow volumes (50% inflow corresponds to 42 CFM or less for weighing with an analytical balance or handling powders; and 35-42 CFM or above for safe air clean up). In cases where insufficient performance was observed, the lack of performance occurred under adverse test conditions or at airflow volumes below minimum recommended airflow volume for the specific operation. Contact Labconco or visit www.labconco.com for complete performance test data. The following provides a brief summary of the findings:

• Smoke purge tests were conducted at airflow volumes of 15-CFM to

84-CFM. The time required to visibly purge the main chamber of smoke varied from approximately 360 seconds at 15-CFM to 60 seconds at 84-CFM.

• Tracer gas was not detected in the breathing zone of the mannequin

during tracer gas containment tests with the glove box vented to the outside. Perimeter scans were also conducted during tracer gas testing of the Protector Filtered Glove Box, and under normal laboratory conditions testing surpassed ASHRAE 110 standards (0.10 PPM average or less) and achieved Labconco standards (0.05 PPM average or less).

• Surrogate naproxen sodium test results were conducted with three different

operators of varied skill level and stature. Each operator weighed 20, one gram samples over a period of 30 minutes or greater. In each case, the operator cleaned up after weighing and no detectable levels of naproxen sodium occurred at the operator, transfer chamber, or in the room. Detection limits were less than 2 ng/cassette or less than 20 ng/cu. meter. The Protector Filtered Glove Box clearly showed excellent repeatability and containment of naproxen sodium powder during typical weighing operations. Test results validated by Safebridge Consultants, Inc.

• Noise Pressure (Sound levels) varied from 51.8 dB(A) at 15-CFM to 68.5

dB(A) at 84 CFM. These sound levels will be much less if the exhaust air from the glove box is ducted to the outside.

• Airborne particulates (measured under positive and negative pressure)

within the main chamber of the glove box exceeded ISO Class 5 conditions (equivalent to Class 100) and achieved ISO Class 3 conditions (equivalent to Class 1).

• Glove box fluorescent lamp light levels (illumination) measured across

the work surface averaged above 80 foot candles for stainless steel models and above 120 foot candles for fiberglass models.

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Chapter 3: Getting Started

• Static pressure measured inside the glove box varied from negative .12

inches of water column at 15-CFM to negative 1.28 inches of water at 84CFM. The total system static pressure as measured between the exhaust filter and the blower varied from negative 0.31 inches of water column at 15-CFM to negative 2.48 inches of water at 84-CFM.

• Displacement (vibration level) measured on the work surface is

negligible at minimum through maximum airflow volume settings. The measured level of displacement ranged from 0.07 x 10-5 meters to 0.38 x 10-5 meters.

• Balance stability test results were excellent with no detectable instability

of an analytical balance to five decimal places and a top loader to three decimal places. Test results were confirmed with the glove box operating at various airflow volume settings and with various sample weights.

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CChhaapptteerr 44:: PPeerrffoorrm

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SSaaffeettyy PPrreeccaauuttiioonnss

Performance Features

The Protector Filtered Glove Box is designed to provide superior particulate containment. The filtered glove boxes have been tested to effectively contain toxic and noxious materials when properly installed and operated. Labconco engineered the filtered glove boxes to minimize the effects of turbulence. The concentrations of particulate materials in the main chamber are determined by the frequency of main chamber air volume changes. See Figure 4-1 to refer to a detailed description of the main performance features.

1. Fiberglass-lined models feature:

• Inlet and outlet HEPA filters, 99.99% efficient on 0.3 micron

particulates.

• Illumination exceeds 120 foot candles.

• One-piece molded fiberglass liner.

• Clear acrylic inner and outer transfer chamber doors.

2. Stainless steel-lined models feature:

• Inlet and outlet ULPA filters, 99.999% efficient on 0.12 micron

particulates.

• Illumination exceeds 80 foot candles.

• Type 304 stainless steel liner.

• Type 304 stainless steel inner and outer transfer chamber doors.

• Type 304 stainless steel front panel.

• Doublewide models include 4 glove ports with main chamber volume

of 34 cu. ft.

All models feature:

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Chapter 4: Performance Features and Safety Precautions

3. Particulate containment <20 nanograms per cubic meter as confirmed by

SafeBridge Consultants, Incorporated.

4. Fast “clean up time.” 0.05 ppm average tracer gas levels or less detected

during perimeter scan testing (surpasses ASHRAE 110 standards) after 5 minute dilution.

5. Airborne particulate cleanliness (measured under positive and negative

pressures per ISO 14644-1 test method) exceeds ISO Class 5 conditions (equivalent to Class 100) and achieves ISO Class 3 conditions.

6. Factory leak-tested with a mass spectrometer while pressurized with

helium at 1 inch of water gauge. No detectable leaks greater than 1 x 10-6 ml/sec.

7. Test port for challenging HEPA or ULPA filter integrity.

8. True Bag-In/Bag-Out Filter Disposal System for safe filter removal and

replacement.

9. LCD display provides real time monitoring of volume dilution rates in

cubic feet per minute (CFM), air changes/minute (ACM), and main chamber pressure (inches of water).

10. Built-in blower with speed control capable of airflow from 15 CFM to 85

CFM.

11. Large viewing area, 36" w x 27.6" high provides maximum visibility.

Viewing window is 3/8" thick, laminated safety glass. Double width models have double the viewing area.

12. Space-saving inner and outer transfer chamber doors that pivot upward,

are counterbalanced and equipped with quick-latches.

13. Interior right-handed side wall-mounted electrical duplex receptacle with

10 amp maximum (100/115 volt models) or 10 amp maximum (230 volt models) circuit load.

14. Interior left hand side wall-mounted pass-through plug for on-site

installation of an optional accessory: electrical outlet, RS-232 port, gas/liquid port, or port for oxygen or moisture monitor.

15. Pair of neoprene gloves, 0.015" thick, 30" long, size 9 ¾. Two pair for

double width models.

16. Switches for light, blower and electrical duplex.

17. Two 8" ID, epoxy-coated aluminum, double-grooved glove ports

mounted 17" apart, with neoprene gaskets. Double width models have four glove ports.

18. Two 25-watt fluorescent lights. Double width models have four lights.

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Chapter 4: Performance Features and Safety Precautions

Figure 4-1

Performance Features

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Chapter 4: Performance Features and Safety Precautions

Safety Precautions

1. It is the responsibility of the user to determine the suitability of this

product for the intended applications. Consult your Safety Officer.

2. Prior to using the glove box, check to make sure that the exhaust

blower is operating and that air is entering the glove box by observing the static pressure gauge.

3. Use good housekeeping in the glove box at all times. Clean up spills

immediately. Periodically clean glove box interior.

4. This product is NOT designed or intended to be explosion proof.

It is the responsibility of the user to determine and avoid lower explosive limits and flammability of any enclosed chemicals or gases. The user is also responsible for taking proper precautions to prevent equipment damage or injury due to explosion or combustion.

5. The use of flammable gases or solvents in the glove box should be

limited. Care must be taken to ensure against the concentration of flammable or explosive gases or vapors. Use of an open flame or exposure to high heat should be avoided in the glove box as heat can damage the fiberglass liner. Heat may harm the filter and damage the filter’s adhesive.

6. Perchloric acid use in this enclosure is prohibited.

7. Radioisotope usage in the glove box should be cleared with your

Safety Officer.

8. A qualified certification technician should test the integrity of the

filters before the box is initially used.

9. The glove box should be recertified whenever it is relocated or

serviced and at least annually thereafter.

10. The use of safety goggles, protective clothing, and any other personal

protective equipment recommended by your Safety Officer should be employed.

11. The HEPA or ULPA filter provides personnel and environmental

protection from particulate matter. Should the blower be reversed to positive pressure inside, this glove box may be used for operations requiring product protection from environmental particulate contamination.

12. HEPA or ULPA filters are only effective for entrapment of particulate

matter. Manipulations that generate gases or vapors from toxic chemicals or radionuclides are not captured by the HEPA or ULPA filters.

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Chapter 4: Performance Features and Safety Precautions

13. The surface of the HEPA or ULPA filter is fragile and should not be

touched. Care must be taken to avoid puncturing the filter during installation or normal operation. If you suspect that a filter has been damaged DO NOT use the glove box; contact a local certification agency or Safety Officer.

14. The filters in the glove box will gradually accumulate airborne

particulate matter from the room and from work performed in the glove box. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the glove box is operating and the nature of work being done in the glove box. With normal usage, the filters will last two to five years before requiring replacement (see Chapter 6).

15. Ensure that the filtered glove box is connected to an electrical service

in accordance with local and national electrical codes. Failure to do so may create a fire or electrical hazard. Do not remove or service any electrical components without first disconnecting the glove box from electrical service.

16. Used HEPA or ULPA filters may be hazardous waste. The user is

responsible for recording chemicals used and disposal of used filters.

17. Ensure only trained operators use the glove box. New users must

review the User’s Manual with emphasis on Routine Daily Work Procedures in Chapter 5 and become familiar with the safe operation of the glove box.

18. Only powders and particulates removed by HEPA or ULPA filters are

appropriate for use in this glove box. Vapors and other gases should be exhausted to the outside or adsorbed with the use of an accessory FilterMate Portable Exhauster equipped with carbon filters. See Chapter 7.

19. Leave the impeller on for at least 5-10 minutes at 50%-100% speed

after work in the glove box has been completed to purge the interior of containments.

20. If a chemical, powder or particulates are spilled on the work surface,

DO NOT switch off the blower until all traces have been removed.

21. Tag glove box with appropriate warning if filters have been removed

or the glove box requires servicing.

22. If the blower fails during use, processes should cease and the area

should be vacated. Inform your Safety Officer immediately.

23. Consult your Safety Officer before removing any tubing that monitors

pressure and airflow or exhaust hoses as they may be contaminated.

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CChhaapptteerr 55:: UUssiinngg YYoouurr GGlloovvee BBooxx

Now that the installation of your glove box is completed, you are ready to use your Protector Filtered Glove Box.

Read this chapter to learn about:

• Starting the Filtered Glove Box

• Control Panel Touch Pad

• All Program Screens

• Routine Daily Work Procedures

• Appropriate HEPA Filter Applications, Suitability and Guidelines

• Definition of Terms

• Appropriate Chemicals for Carbon Filters

• Acid Use

Starting the Filtered Glove Box

1. To start the glove box blower, turn the “Blower” switch to on and

adjust the “Airflow” speed control.

2. To turn the fluorescent light on, turn the “Light” switch to on.

Note: The glove box doors must be completely closed before performing work.

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Chapter 5: Using Your Glove Box

Control Panel Touchpad

The touchpad of the Protector Filtered Glove Box is shown below. Take a moment to get familiar with the buttons, their location and function. Also familiarize yourself with the display located on the front. The display will report system functions, such as airflow status, pressure status, timer displays, low airflow alarm messages, and low pressure alarm messages.

Figure 5-1

Menu Button – This button toggles the display between menu modes. When

in the menu mode, this button returns you to the previous menu.

Mute Button

Select Up/Down Yes/No Buttons

values in the menu mode.

Enter Button

menu mode.

Timer Button

elapsed timer.

– Allows you to temporarily mute an alarm.

– Allows you to change options and

– Allows you to enter or select a value or select an option in

– Allows you to select either a repeating interval timer or an

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All Program Screens

Chapter 5: Using Your Glove Box

4 KEYS

MENU, ▲, ▼, ENTER

Press MENU ke

MENU OPTIONS ▲▼ to Choose, ENTER to Select, MENU to Exit

1. Setup

2. Timer

CLOCK TIMER ▲▼ to Choose, ENTER to Select, MENU to Exit

1. Interval Timer

2. Elapsed Timer

SETUP

1. Units of Measure

2. Security Lock

3. Calibration

4. Restore Defaults

5. Diagnostic

Enter

UNITS OF MEASURE ▲▼ to Choose, ENTER to Select, MENU to Exit

1. Metric (MPS) f 2. US (FPM)

SECURITY LOCK

*Security Lock Code 234.

Security Lock can prevent unauthorized personnel from changing settings. When Security Lock is ON, the unit will only allow the user to enter in a security lock.

▲▼ to Choose, ENTER to Select, MENU to Exit

00:00:00

▲▼ to Choose, ENTER to Select, MENU to Exit

00:00:00

▲▼ to Choose, ENTER to Select, MENU to Exit

*XXX

SECURITY LOCK ▲▼ to Choose, ENTER to Select, MENU to Exit

1. OFF

2. ON

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Chapter 5: Using Your Glove Box

Main Setup Screens

SETUP (cont.)

1. Units of Measure

2. Security Lock

3. Calibration

4. Restore Defaults

5. Diagnostic

Enter

CALIBRATION LOCK ▲▼ to Choose, ENTER to Select, MENU to Exit

RESTORE DEFAULTS

All User Data will be reset except calibration.

UP to Select MENU to Escape

DIAGNOSTIC TEST ▲▼ to Choose, ENTER to Select, MENU to Exit f 1. Keypad

2. Airflow Sensor

3. Pressure Sensor

Enter

Certifier’s Use Only!

234 code to access

calibration mode.

KEY PAD TEST

All Keys Are Off.

AIRFLOW SENSOR X1: 258/258 X5: 218/218 X5 CAL: 135/218

Airflow: 79/ 80 CFM

PRESSURE SENSOR Press: X1: 286 CAL: 258/286

Pressure: -1.99/1.97 IOW

See “Diagnostics Program Screens” for more info.

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Chapter 5: Using Your Glove Box

Calibration Program Screens

CALIBRATION LOCK

?? to Choose, ENTER to Select, MENU to Exit

Enter code for certification use only and then press

ENTER key

IRFLOW SENSOR

. Zero Adjust

2. Level Adjust

IRFLOW SENSOR UTO-ZERO

1. Disable

2. Enable

Enable

IRFLOW SENSOR ZERO

Please Wait:

IRFLOW Set to Known ?? to Choose, ENTER to Select, MENU to Exit

irflow:

CALIBRATION

?? to Choose, ENTER to Select, MENU to Exit

1. Airflow Senso

irflow Alarm

3. Pressure Senso

4. Pressure Alarm

5. Box Size

6. Box Type

BOX SIZE

. Single

2. Double

BOX TYPE

1. Positive Pressure

2. Negative Pressure

PRESSURE ALARM

larm Level

2. Alarm Delay

IRFLOW ALARM

larm Level

2. Alarm Delay

PRESSURE SENSOR

1. Zero Adjust

2. Level Adjust

?? to Choose, ENTER to Select, MENU to Exit

larm Level: 10 CFM

larm Level is best set at low levels such

as 10 CFM below oper

?? to Choose, ENTER to Select, MENU to Exit

larm Level: x

PRESSURE SENSOR

ZERO ADJUST Open all doors Enter when done

PRESSURE SENSOR

LEVEL ADJUST Set known Pressure ?? to Choose, ENTER to Select, MENU to Exit

Pressure: X.XX IOW

PRESSURE ALARM ?? to Choose, ENTER to Select, MENU to Exit

larm Level: X.XX IOW

LARM LEVEL

LARM DELAY

Pressure Sensor Auto-Zero Note: Every time blower is shut off, wait 100 seconds and thentake zero data for 30 seconds.

uto- Zero occurs while the blower is off for the entire time span. The “span” or difference between zero airflow and 70

is saved from “Pressure Sensor” calibration in step 2. Also, flash icon on LCD to shut Auto-Zero.

IRFLOW SENSOR ZERO

Please Wait:

150

SENSOR LEVEL ADJUST

irflow

CFM

ating speed.

Seconds

PRESSURE SENSOR

ZERO ADJUST

Wait

PRESSURE SENSOR

LEVEL ADJUST

Wait 150 Seconds

?? to Choose, ENTER to Select, MENU to Exit

larm Level: xxx Seconds

Seconds

150

LARM DELAY

CFM

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Chapter 5: Using Your Glove Box

Diagnostic Program Screens

DIAGNOSTIC TEST ▲▼ to Choose, ENTER to Select, MENU to Exit f 1. Keypad

2. Airflow Sensor

3. Pressure Sensor

KEY PAD TEST

All Keys Are Off.

AIRLFOW SENSOR X1: 261/XXX X5: 212/XXX X5 CAL: 134/212 Airflow: 70/70 CFM

Airflow Sensor Diagnostic Notes: X5 CAL example represents a pressure of 134 with the blower off and 212 with the blower speed to produce 70 CFM for this calibration example. XXX, 134, and 212 are calibrated values for this example.

PRESSURE SENSOR Press: X1: 286 CAL: 258/286

Pressure: -1.99/1.97 IOW

Pressure Sensor Diagnostic Notes: X1 example indicates raw voltage level of pressure sensor at present conditions. CAL example represents a raw pressure of 258 with the doors in the glove box open and no pressure inside the glove box and a raw pressure of 286 when the negative high pressure level was calibrated at -1.97 inches of H (IOW). Press example shows a current pressure of -1.99 IOW and the unit’s high calibration point of

1.97 IOW.

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Chapter 5: Using Your Glove Box

Routine Daily Work Procedures

Planning

• Thoroughly understand procedures and equipment required before

beginning work.

Start-up

Loading Materials and Equipment

Work Techniques

• Consult your Safety Officer for personal protective equipment

recommendations.

• Turn on exhaust blower and light.

• Allow the enclosure to operate for 2-5 minutes at 50-100% blower

speed.

• Load only the materials required for the procedure. Do not overload

the glove box.

• Be aware that gloves will inflate when the glove box is operated

under negative pressure. Ensure items in the glove box will not obstruct the movement of the gloves.

• After loading, wait 2-5 minutes at 50-100% blower speed to purge

airborne contaminants from the work area before beginning procedures.

• Segregate all clean and contaminated materials in the work area.

Final Purging

• All open trays, weigh vessels or containers must be sealed before

being removed from the glove box.

• Objects in contact with contaminated material must be surface

decontaminated before removal from the glove box.

• The interior surfaces should be cleaned per user’s protocol.

• Upon completion of work, the glove box should be allowed to operate

for 5 minutes undisturbed at 100% speed or 10 minutes at 50% speed to purge and dilute airborne contaminants from the work area.

• After purging the main chamber, the transfer chamber should also be

purged for 5 minutes at 100% speed or 10 minutes at 50% speed with the inner door open, before removing sealed material.

Shutdown

• Turn off the exhaust blower and light.

• Keep both doors closed when not in use.

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Chapter 5: Using Your Glove Box

Appropriate HEPA or ULPA Filter Applications, Suitability and Guidelines

• Microbiological materials and hazardous solids containment protecting

only the operator and the surrounding environment.

• Weighing of powders.

• Containment of hazardous particulates using a HEPA or ULPA filter.

• Powder or particulate procedures traditionally performed on an open

bench.

• The filtered glove box provides personal and environmental protection from

particulate matter, hazardous powders and microbiological materials.

• This glove box can be used for sample protection by reversing the

blower airflow direction, causing the main chamber to be under positive pressure. Positive Pressure Conversion Kit is required (see Chapter 7).

• Manipulations that generate gases or vapors, i.e., toxic chemicals or

radionuclides, require the use of a HEPA or ULPA and carbon filter in combination. Otherwise the glove box should be ducted to the outside.

• Each filtered glove box is provided with two HEPA or ULPA filters.

Follow the Filter change procedure in Chapter 6 for disposal instructions. For microbiological materials, follow the Decontamination Procedure in Chapter 6 for disposal instructions.

Definition of Terms

NIOSH – National Institute for Occupational Safety and Health/Mine Safety and Health Administration. (U.S.A.)

TWA – Recommended Exposure Limits expressed as a Time Weighted Average. The exposure limit for that chemical for up to a 10-hour workday, 40 hours a week. Expressed in units of parts per million or milligrams per cubic meter.

Odor Threshold – The value in parts per million or milligrams per cubic meter for which one might expect to smell a chemical’s presence in the air. This value is very subjective and detection will vary with the sensitivity of one’s nose. The period of time until the odor threshold is reached in the exhaust stream can be estimated from Labconco exclusive computerized filter modeling program. Contact Labconco on carbon filter life for specific applications. See Chapter 6.

Saturation Level or Time – There is a limit to the amount of chemical that can be adsorbed by activated carbon, or neutralized by chemically-treated carbon. Once the capacity of the carbon is reached, it is considered to be saturated and will adsorb (or neutralize) no further material; the outlet concentration of the chemical will equal the inlet concentration from that

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Chapter 5: Using Your Glove Box

point until the filter is replaced. (Note that the capacity of activated carbon is not a constant, but varies with the inlet concentration). Labconco Technical Specialists can determine with the computerized carbon-modeling program the estimated time saturation for a particular chemical. When using a HEPA or ULPA filter in all filtered glove boxes or in combination with a carbon filter, the speed control will need to be increased to allow for filter loading.

IDLH (Immediately Dangerous to Life and Health). An atmosphere that poses an immediate hazard to life or produces immediate irreversible health effects. IDLH concentrations should not be approached in the glove box.

Appropriate Chemicals for Carbon Filters

Below is a general set of rules to determine appropriateness of chemical usage. See Chapter 7 to attach and order an accessory FilterMate Portable Exhauster equipped with carbon filters.

Selected organic chemicals considered to be occupational

carcinogens by NIOSH can be used in the filtered glove box with carbon filters under rigid restrictions. See separate discussion on carcinogens for special instructions.

Organics must have time weighted exposure limits (TWA) of 1 PPM or greater.

Chemicals must have a detectable odor at concentrations below the TWA for the chemical.

Chemicals must be designated by NIOSH guidelines as acceptable for use with chemical cartridge-type respirators (the exception is formaldehyde and ammonia/amines, which use impregnated carbon). Chemicals not listed by NIOSH in the Pocket Guide must be approved by Labconco Product Specialist (or Engineering).

Inlet concentration must never exceed the IDLH (Immediately Dangerous to Life and Health) concentrations.

Chemicals having a recommendation by NIOSH of at least “Escape GMFOV” (Gas Mask Full-Face Respirator).

When evaporating a mixture of chemicals, the chemical having the lowest TWA will be used to determine if the mixture meets the guidelines.

Call a Labconco Product Specialist at 1-800-821-5525 for assistance in chemical appropriateness and use of an accessory FilterMate Portable Exhauster with your glove box.

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Chapter 5: Using Your Glove Box

Prohibited Acid Use

The Protector Stainless Steel Filtered Glove Box and internal metal parts must not be exposed to mineral acid use. No exceptions are permitted, as the glove box life span will be limited with mineral acid use. The door latch metal components and other metal parts inside the Protector Fiberglass Filtered Glove Box are susceptible to mineral acid use. However, the fiberglass liner by itself has superior chemical resistance over the stainless steel liner.

NOTE: If mineral acids are used with the Protector Stainless Steel

Filtered Glove Box, Labconco makes no claims about corrosion resistance and warranties will become void.

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CChhaapptteerr 66:: MMaaiinnttaaiinniinngg YYoouurr GGlloovvee BBooxx

Review this chapter on maintenance for the following:

1. Routine maintenance.

2. Decontamination.

3. Determining when to replace the filters.

4. How to install new filters.

5. HEPA or ULPA filter leak test.

6. Initial certification.

7. Re-certification.

8. Fluorescent light replacement.

9. Motorized impeller replacement.

10. Speed control replacement.

11. Airflow/Pressure monitor circuit board replacement.

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Chapter 6: Maintaining Your Glove Box

Routine Maintenance Schedule

Weekly

• Wipe down the interior surfaces of the glove box with a disinfectant

or cleaner, depending upon the application.

• Using a damp cloth, clean the exterior surfaces of the glove box,

particularly the front and top, to remove any accumulated dust.

• Operate the exhaust system, noting the airflow through the glove box

to effectively remove a source of visible smoke.

Monthly (or more often as required)

• Chart the operating static pressure at a typical speed control setting.

Change filters when the static pressure increases two-fold or airflow decreases two-fold.

Annually

• Replace the fluorescent lamps.

• Have the glove box re-certified by a qualified certification technician.

See Certification and Recertification in Chapter 6.

Decontamination

When used in conjunction with microbiologicals, the glove box should be decontaminated with formaldehyde gas before:

• Performing maintenance work in contaminated areas

• Filter changes

• Moving the glove box to a new location

• Changing research programs

The procedures for performing a gaseous decontamination are thoroughly outlined in the U.S. Department of Health, Education and Welfare booklet entitled Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets, available from NIH, Division of Safety, Bethesda, MD 20892, call 301-496-2801.

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Chapter 6: Maintaining Your Glove Box

Determining When to Replace Filters

The HEPA or ULPA filters in the filtered glove boxes gradually accumulate airborne particulate matter and powders from the enclosure and room. The rate of accumulation will depend upon the cleanliness of the room air, the amount of time the enclosure is operating, and the nature of work being done in the enclosure. In typical installations and usage, the filters will last two to five years before requiring replacement. Replace filters if the speed control is adjusted to full speed and the airflow is half its normal starting point. Replace filters if it fails the HEPA or ULPA Filter Leak Test in Chapter 6.

How to Install New Filters

The Protector Filtered Glove Boxes include a true bag-in/bag-out filter disposal system for safely removing the filter and protecting the worker from toxic powders and particulates (see Figure 6-1, Filter Changing Diagram). The accessory bags can be ordered from Labconco (see Chapter 7). Two bags will be required for each HEPA or ULPA filter for a total of 4 each to complete the change process. For your safety, wear appropriate personal protective equipment during the change of filters. If working with microbiologicals, use the decontamination procedure referenced above. (Consult your Safety Officer before performing filter changes).

1. Turn the glove box OFF. Refer to Appendix A for replacement parts

and Appendix B for dimensions. Remove the top metal cover.

2. Loosen the six filter nuts that secure each filter.

3. Place the accessory bag over the filter and seal to the filter housing.

4. Place both arms in the rear gloves of the bag, and carefully draw the

filter out of the glove box. Rest the filter on the floor, table or cart. Remove arms from bag gloves.

5. Create two seals between the glove box filter housing and the old

filter. Seals can be heat sealed or sealed with tie wraps. Cut off the bag between the two seals and leave the bag stub on the filter housing port to the glove box.

6. Place the new filter in another new bag. Then place the new bag with the

new filter over the old bag stub and onto the glove box filter housing.

7. Carefully remove the old bag from the filter housing using the single glove

near the access port. Draw the old bag and stub into the single glove and turn the glove inside out. Double seal this glove on the contaminated bag stub inside. Remove the bag and dispose of properly.

8. Carefully install the new filter and uniformly tighten the six filter nuts

until the filter gasket is compressed 50%. Then roll up the second filter bag, secure with strap and leave in place for the next filter replacement procedure.

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Chapter 6: Maintaining Your Glove Box

9. Turn the glove box ON and proceed to the Filter Leak Test in Chapter 6.

10. Reinstall the top metal cover.

Figure 6-1

Filter Changing Diagram

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Chapter 6: Maintaining Your Glove Box

HEPA or ULPA Filter Leak Test

Purpose

This test is performed to determine the integrity of the filter(s), the filter housing, and the filter mounting frames. The leak test is to be done by a qualified technician with calibrated equipment. Reference Leak Testing and Photometer scanning from the Institute of Environmental Services (IES-RPCC001.3)

Equipment Required

1. An aerosol photometer ATI model 2D, 2E, 2G or equivalent. Air

Techniques Hamilton Associates Inc. 11403 Cron Ridge Dr., Owings Mills, MD 21117

2. One aerosol generator of the Laskin nozzle(s) type. An aerosol of

mineral oil or suitable liquid shall be created by flowing air through it. The compressed air supplied to the generator should be adjusted to a pressure as indicated in the procedure. Air Techniques Inc. Model TDA4A or equal.

3. Mineral oil (Labconco P/N 1491400).

4. Sampling Nozzle, Rectangular 1/2" x 3-1/4", Air Techniques, Inc.

Procedure

When Using the ATI 2G Photometer

NOTE: Because the downstream side of the HEPA or ULPA filters cannot be scanned, a downstream average must be taken.

1. Turn on the photometer and allow it to operate for a minimum of 5

minutes. Leave the valve in the “CLEAR” setting.

2. Press the “ENTER” keypad. Press the “REF” keypad.

3. The display will show “P1” or “P2” for approximately 1 second, and

then display a numerical value.

4. Using the “▲” or “▼” keypads, increase or decrease the numerical

value, respectively, until it equals 68. (Set for 100 CFM, adjust for other airflows.)

5. Press the “ENTER” Keypad. The photometer will scan for 15

seconds, and then the “0” keypad will flash. Press the “Enter” keypad. The unit will scan for 5 seconds, the display will read “0000,” and the unit will sound a confirming tone.

6. Set the valve to “DOWNSTREAM.” Place the palm of your hand

over the sampling port of the pistol. There should be a strong vacuum at this port. If the vacuum is weak, contact Air Techniques Hamilton Associates.

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Chapter 6: Maintaining Your Glove Box

7. Ensure that the glove box speed control is adjusted to the maximum

setting and the blower is exhausting 55-100 CFM through the glove box.

8. Position the aerosol generator discharge near the internal opening of

each filter. Check both filters.

9. Start the aerosol generator. Ensure that the generator is operating

with 1 nozzle on at 10 PSIG.

10. Allow the generator to operate for a minimum of 15 seconds. Place a

1/4" OD sampling tube in the exhaust downstream of the filters. The inlet filter can be checked from the test port on the exhaust filter and the exhaust filter can be checked from the blower inlet (suction side) test port. Connect this tube to the “downstream” sampling port of the photometer.

Acceptance

The downstream average penetration shall not exceed 0.005 percent as measured by the photometer.

Procedure When Using the ATI 2-D or 2-E Photometer

NOTE: Because the downstream side of the HEPA or ULPA filters cannot be scanned, a downstream average must be taken.

1. Turn on the photometer and allow it to operate for a minimum 5

minutes. Leave the range switch at “100%” and the valve in the “CLEAR” setting.

2. Use the screw on the face of the gauge to adjust the meter to 0 if

necessary.

3. If the “INT REF” switch is a square button, depress it. The switch will

remain depressed and illuminated. If the switch is a toggle type switch, push and hold the switch to the “ON” position. Keep this switch in the “ON” position through step 4.

4. Turn the “GAIN” knob until a value of 15 is obtained. (Set for 100

CFM, adjust for other airflows.)

5. Turn the range switch to 0.1. Turn the Stray Light knob until the

meter reads “0.”

6. Turn the valve to the “DOWNSTREAM” setting. Place the palm of

your hand over the sampling port of the pistol. There should be a strong vacuum at this port. Return the valve to the “CLEAR” setting.

7. Ensure that the glove box speed control is adjusted to the maximum

setting and the blower is exhausting 100 CFM through the glove box.

8. Position the aerosol generator discharge near the opening of each

filter. Check both filters.

9. Start the aerosol generator. Ensure that the generator is operating

with 1 nozzle on at 10 PSIG.

10. Allow the generator to operate for a minimum of 15 seconds. Place a

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Chapter 6: Maintaining Your Glove Box

test port. Connect this tube to the “downstream” sampling port of the photometer.

Acceptance

The downstream average penetration shall not exceed 0.005 percent as measured by the photometer.

Initial Certification

The Protector Filtered Glove Box has been leak tested at the factory with helium gas at positive 1" w.g. pressure; leaks cannot exceed 1 x 10 The Filter Leak Test has been performed at the factory on both filters with no particulate leaks greater than .005 percent. The filtered glove box should be certified before use for the three tests listed under Re-Certification below. It is also recommended to perform the Filter Leak Test again should there be any damage caused during transport.

-6

cc/sec.

Re-Certification

Under normal operating conditions, the glove box should be re-certified at least annually if serviced. The certifier should perform the following tests.

• Record airflow volume and glove box static pressure. Replace filters if

pressure doubles or airflow is reduced to half the original volume.

• Perform Filter Leak Test.

• Perform a Smoke Test to determine proper dilution rate inside the glove

box. Smoke removal should be completely purged in less than two minutes at full blower speed.

Fluorescent Light Replacement

1. Disconnect the power. Refer to Figure 6-2 for replacement.

2. Locate the upper front panel.

3. Remove the front panel by removing two screws located at the bottom

of the front panel on each side.

4. Remove the old fluorescent lamps.

5. Reinstall the new fluorescent lamps and front panel by reversing the

steps above.

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Chapter 6: Maintaining Your Glove Box

Fluorescent Bulbs

Front panel screw location

Fluorescent Bulb Spring Clip

Fluorescent Bulb Light Socket

Figure 6-2

Lights Replacement

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Chapter 6: Maintaining Your Glove Box

Motorized Impeller Replacement

The motorized impeller must be replaced as a complete unit. See Appendix A for replacement parts diagram. Consult your Safety Officer for proper decontamination or sealing of tubing before replacing the blower.

1. Unplug the motorized impeller from the electrical outlet. Refer to

Appendix A for replacement parts.

2. Remove the hose connecting the exhaust filter to the motorized impeller.

3. Remove the motorized impeller and support braces.

4. Label and remove the tubing as shown in Figure 6-3.

5. Reassemble the new motorized impeller by reversing the steps above.

WARNING: High-speed blower. Never operate impeller

with housing off.

Motorized Impeller Assembly

Rear Port Blower Static Pressure S.P.

Front Port Blower Total Pressure T.P.

T.P.

Silicone Larger I.D. Tubing

S.P.

Connection

Hose

Exhaust Filter

Housing

Main Chamber Static Pressure S.P.

Motorized Impeller & Tubing

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Figure 6-3

Chapter 6: Maintaining Your Glove Box

Speed Control Replacement

1. Disconnect the power and remove the front control panel where the speed

control is located.

2. Remove the two fasteners holding the speed control. Refer to Appendix

A for replacement parts.

3. Disconnect all wires leading to the speed control as shown in Figure 6-4.

Connect wires on new speed control in the same position as the old speed control.

4. Reassemble to the system in the same position and with the same

fasteners that were removed earlier.

Airflow/Pressure Monitor Circuit Board Replacement

1. Disconnect the power. Refer to Figure 6-4.

2. Remove the control panel supported by four screws.

3. Remove the circuit board from the control panel and replace with the new

circuit board.

4. Replace all the tubing as shown in Figure 6-4.

5. Reinstall the control panel with the new circuit board.

6. Calibrate the glove box airflow and pressure per the Calibration

procedure in Chapter 5.

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Chapter 6: Maintaining Your Glove Box

Typical Circuit Board Mounting Screw

Blower Total Pressure, T.P., Port Closest to Circuit Board (Larger Sensor)

Typical Circuit Board Connector

Silicone Larger I.D. Tubing

Main Chamber Static Pressure, S.P., Port Closest to Circuit Board (Smaller Sensor)

Speed Control and Wires

Blower Static Pressure, S.P., Port Away from Circuit Board (Larger Sensor)

Airflow/Pressure Monitor Circuit Board and

Speed Control Replacement

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Figure 6-4

CChhaapptteerr 77:: AAcccceessssoorriizziinngg YYoouurr GGlloovvee BBooxx

There are many ways to accessorize and modify the glove box for your individual requirements. These include many different accessories listed in this chapter.

1. Black Epoxy Flat Work Surface (Part #4882807)

A solid epoxy resin 60" x 30" x 1.25" work surface is available to support the glove box.

2. 30" Standard Base Cabinet (Part #9900200)

Two optional base cabinets may be ordered to support the 60" x 30" work surface and glove box. Contact Labconco for additional sizes.

3. Adjustable Height Base Stands with Attached Work Surface

(Part #5235500 w/casters, Part #5235501 w/adjustable feet, Part #5062020 Double Mobile Base Stand)

These stands, 60" x 31", with attached black laminate work surfaces have an adjustable height range from 33" to 40" and include a lower shelf for supporting accessory equipment. See Appendix B for dimensions of Double Mobile Base Stand for double width glove boxes.

4. Blower Foot Switch (Part #5441300)

Provides a hands-free means to shut off power to the built-in blower.

5. Bag Set for Bag-in/Bag-out HEPA Filter Replacement (Part #5241700)

Set of two convenience bags and two safety straps for changing and disposing of one filter safely with a bag-in/bag-out procedure.

6. Anti-Static Ionizer Fan (Part #5234400, 100/115V or

Part #5234401, 230V)

Used for weighing operations. The ionizer fan circulates ionized air inside the glove box to reduce static charge. Ionizer Replacement Points (set of 8 emitter electrodes) are ordered separately (Part #5235100). See Appendix D for additional information.

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Chapter 7: Accessorizing Your Glove Box

7. Balance Vibration Isolator (Part #5234600)

Isolates building vibration and aids with balance weighing. Includes a 20" x 13" x 2 1/4" marble slab with isolator pads and type 304 stainless steel top cover to protect the porous marble.

8. Add-A-Valve Kit (Part #5442600 for Transfer Chamber and Part

#5442700 for Main Chamber)

Includes the two valves, tubes, instructions and nuts to supply the main chamber or the transfer chamber to purge vacuum, fill gas, connect a drying train, or connect filtration components.

9. Vacuum Aspirator Kit

Includes the valve, hose, canister, and vacuum pump for evacuating fluids around the work surface.

Part # Descriptions

5241800 Vacuum Aspirator Kit, 115V 5241801 Vacuum Aspirator Kit, 230V

10. Electrical Power Strips

These convenience electrical power strips are available in US and international versions and plug into the interior receptacle of the glove box.

Part # Descriptions

5211500 US Power Strip 115V, 4 Position, 10A 5210601 UK Power Strip 250V, 6 Position, 10A 5290401 European Power Strip 250V, 4 Position, 10A 5332601 Australian Power Strip 250V, 6 Position, 10A

11. Electrical 9 Pin Sealed Pass-Through Kit (Part #5075619)

Includes sealed 9 pin (D-subminiature with male pins on one side and female pins on the other) connector and cord for data transfer to a typical printer outside the glove box from the balance. Note: Order the 9 pin installation kit separately which includes all the drill bits and hole saws for field installation (Part #5241100).

12. Mechanical or Electrical Straight Pass-Through Kit

(Part #5240700)

Provides three ID pass-through ports with sizes of .230/.395, .230/.530, and .450/.705. Order the installation kit with drill bits separately (Part #5240800). Useful for sealing cords and plumbing.

13. FilterMate Portable Exhausters and Filters for Secondary

Filtration (see models and filters below)

The FilterMate Portable Exhauster can successfully adsorb low level organic vapors, formaldehyde, ammonia, and secondary particulates via another HEPA filter.

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Chapter 7: Accessorizing Your Glove Box

Part # Descriptions

3970000 115V FilterMate (HEPA only included) 3970020 230V FilterMate (HEPA only included) 3970001 115V FilterMate (Carbon Filter sold separately) 3970021 230V FilterMate (Carbon Filter sold separately) 3970002 115V FilterMate w/thimble (HEPA included) 3970022 230V FilterMate w/thimble (HEPA included) 3970003 115V FilterMate Combination HEPA/Carbon (order

Carbon Filter separately)

3970023 230V FilterMate Combination HEPA/Carbon (order

Carbon Filter separately) 3970004 115V FilterMate (Two Carbon Filters Sold separately) 3970024 230V FilterMate (Two Carbon Filters sold separately) 3707900 HEPA Filter for FilterMate 3923400 Organic Carbon Filter for FilterMate, 12 lbs. 3923401 Formaldehyde Carbon Filter for FilterMate, 14 lbs. 3923402 Ammonia Carbon Filter for FilterMate, 16 lbs. 3776002 Replacement Bag to Bag-In/Bag-Out HEPA Filter

14. 5" Thimble Exhaust Connector for Connection to the FilterMate

(Part #5441000)

Connects to the top of the blower on the glove box for attaching the FilterMate Portable Exhauster. The thimble is necessary for balancing the blower in the glove box with the FilterMate Portable Exhauster.

15. 6" Thimble Exhaust Connection to Building Exhaust (Part #5440900)

Provides a 6" exhaust thimble connection to the blower on the glove box for exhausting to the outside. The thimble is necessary for balancing the blower on the roof with the glove box blower.

16. 6" Hard Duct Connection to House Exhaust (Part #5440800)

Provides a 6" hard duct connection for using a roof-mounted blower and by-passing the blower on the glove box.

17. Remote Wall-Mount Blower Kit (Part #5229800)

Provides a wall-mount bracket, 4" dia. x 6' long hose, hose clamps, and 8' power cord for moving the blower from the glove box and remotely mounting it to a wall within 5'.

18. 6" Exhaust Dampers

Exhaust dampers allow airflow adjustments to be made, maintaining proper airflow to roof-mounted remote blowers or building exhaust.

Part # Descriptions

3924000 6" Epoxy-Coated Steel In-Line Adjustable Damper 4724200 6" PVC In-Line Adjustable Damper

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Chapter 7: Accessorizing Your Glove Box

19. Remote Blowers

Has a 1/4 hp direct drive motor and corrosion-resistant epoxy-coated steel housing and wheel with blower inlet of 6.00" ID. Outlet dimensions are 4.25" x 7.38" OD.

CFM @ Static Pressure-Inches of H2O S.P. 0.0" 0.125" 0.25" 0.50" 0.75" 0.87" CFM 595 560 515 420 300 167

Part # Description 4863500 Remote Blower, 115 V, 60 Hz. 4.4 amps 4863501 Remote Blower, 115/230 V, 50 Hz, 5.6/2.8 amps

Figure 7-1

All dimensions in inches

20. Positive Pressure Conversion Kit (Part #5441200)

Converts integral glove box blower from negative pressure operation to positive pressure.

21. Recirculation Kit (Part #5441100)

Allows the blower exhaust to be connected to the HEPA inlet filter for recirculation of the exhaust air. Useful with drying train components on the main chamber.

22. Filtration Components for Moisture and Fume Removal

The following components are useful in removing organics, acids, ammonia, moisture, and other gases as indicated. They are connected to the glove box using the #5442600 or 5442700 Add-A-Valve Kit.

Part # Descriptions

5248200 Vacuum Pump for Circulation, 115V, 60 Hz w/switch 5248201 Vacuum Pump for Circulation, 230V, 50 Hz 7815301 Canister with Stand for Traps 7815200 Moisture & Solvent Trap Insert (molecular sieve) 7814800 Acid Vapor Trap Insert 7815000 Radioisotope Trap Insert

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Chapter 7: Accessorizing Your Glove Box

Part # Descriptions

7995600 Ammonia Trap Insert 5244100 Flowmeter, Visual 5240100 Gas & Vacuum 0.2 Micron Filter

23. Polycarbonate Viewing Window with Frame (Part #5066700)

Polycarbonate window, for those users that prefer polycarbonate over the standard laminated safety glass (includes metal frame).

24. Interior Glove Port Cover (Part #5060400)

Quickly seals glove box should sudden leak occur due to glove damage. Stainless steel with molded rubber gasket and spring-loaded latches with adjustable closure knob.

25. Interior Shelves (Part #5061600)

Provide shelf space across back wall of glove box interior. Three stainless steel storage shelves are attached to epoxy-coated steel upright supports. Shelves have adjustable height and measure 30" wide x 6" deep. Installation hardware and instructions included.

26. Gloves (see Appendix A: Replacement Part numbers for other

glove sizes and material types)

Neoprene gloves are the most resistant to abrasion and tearing. Butyl gloves provide higher impermeability and improved dexterity. Hypalon gloves are made with a mixture of neoprene and butyl rubber to provide an intermediate level of neoprene and butyl features.

27. External Glove Port Covers (Part #5242200)

Cap and clamp attaches to the glove ports to eliminate glove inflation during purging and filling operations and reduce the cycle time of the purging/filling process. Set of two.

28. Left side Sealed Electrical Plug Kit (see Part #’s below)

Part # Descriptions

5075622 115V single outlet 5075623 230V IEC single outlet

29. DOP Test Kit for Filters (Part #5242400)

Includes the connections and hoses to carry out the Filter Leak Test documented in Chapter 6 of this manual.

30. Drying Train Tubing Kit

Includes the (FEP) tubing and plumbing fittings for connecting typical moisture, and other gas fume removal components (see item 23 above). Fluorinated ethylene-propylene (FEP) tubing has very low moisture absorption.

Part # Descriptions

5242500 Drying Train Tubing Kit; Moisture, Solvents,

and Radioisotopes

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Chapter 7: Accessorizing Your Glove Box

31. HEPA and ULPA Filters

Part # Descriptions

5225105 Replacement Standard HEPA Filter, 99.99%

efficient on 0.3 micron particles

5225106 Optional ULPA Filter, 99.999% efficient on 0.3

micron particles

32. Inlet-Outlet Connection Kit – No HEPA (Part #5253400)

The 3.94" OD inlet-outlet connection kit consists of two sealed flange connections that attach to the inlet and outlet vent holes of the glove box main chamber when the HEPA filter housings and HEPA filter are removed. The connection kit enables direct connection of 4.0" ID hose or ductwork to the glove box.

33. Left Side Door Option (Factory Prepared Only)

Any stainless steel glove box can be factory prepared with a large sealed left side door for use in conveniently loading large equipment. With the left side door option, the need to remove the front laminated safety glass frame is eliminated. The left side door has an opening of

16.5" (419 mm) wide x 23.2" (589 mm) high. Contact Labconco at time of order for the left side door option.

Left Side Door Option

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Figure 7-1

CChhaapptteerr 88:: TTrroouubblleesshhoooottiinngg

Refer to the following table if the Protector Filtered Glove Box fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance.

PROBLEM CAUSE CORRECTIVE ACTION

Blower won’t operate.

Blower and lights won’t operate.

Lights do not work.

No power to electrical receptacle.

Airflow/Pressure Monitor will not operate.

Unit not plugged into outlet. Plug the glove box into appropriate electrical

service. Circuit breaker(s) or Ground Fault Interrupter. Blower wiring is disconnected. Blower switch is defective. Replace switch if no voltage at blower. Motorized impeller or blower is defective. Unit not plugged into outlet. Plug unit into appropriate electrical service.

Circuit breaker(s) tripped. Reset or replace circuit breaker. Lamp not installed properly. Inspect lamp installation. Lamp wiring disconnected. Inspect lamp wiring. Defective lamp. Replace lamp. Light switch is defective. Replace light switch. Defective electronic ballast. Replace ballast. Unit not plugged in. Plug unit into electrical service. Circuit breaker(s) tripped. Reset or replace circuit breaker. Receptacle switch is defective. Receptacle is defective. Replace receptacle. Unit not plugged into outlet. Circuit breakers tripped. LCD Display is defective. Replace circuit board. Alarms, airflow, or pressure not calibrated.

Reset circuit breaker.

Inspect blower wiring per wiring diagram on

unit.

Replace motorized impeller or blower. See

Chapter 6.

Replace receptacle switch.

Plug unit into appropriate electrical service.

Reset or replace circuit breaker.

Calibrate the monitor per the menus and

procedure in Chapter 5.

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The components that are available for your glove box are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service.

Item Qty. Part Number Description

1A 1 5066600 Window, Glass Viewing 1B 1 5066700 Window, Polycarbonate Viewing 2A 1 5067200 Window, Gasket, Fiberglass Models 2B 1 5098400 Window, Gasket, Stainless Steel Models 2C 0.01 quart 1576800 Adhesive, Plyobond, Fiberglass Models 2D 0.01 quart 1594800 Adhesive, 3M #4799, Stainless Steel Models 3A 1 pair 1640600 9 ¾ size Neoprene Glove (standard) 3B 1 pair 1640601 9 ¾ size Butyl Gloves 3C 1 pair 1640602 9 ¾ size Hypalon Gloves 3D 1 pair 1640500 8 ½ size Neoprene Gloves

3E 1 pair 1640501 8 ½ size Butyl Gloves

3F 1 pair 1640502 8 ½ size Hypalon Gloves 3G 2 each 1640000 O-rings 3H 2 each 1965600 Clamp – O-Ring Gloves

4A 1 5410800 Complete Door Assy., Fiberglass Inner 4B 1 5098500 Complete Door Assy., Stainless Steel Inner 5A 1 5422700 Inner Acrylic Door Only, Fiberglass Models Only 5B 1 5064200 Door Gasket, Inner and Outer, Fiberglass Models Only 5C 0.1 tube 1594800 Gasket Sealant, Fiberglass Only

6 1 5410000 Outer Acrylic Door Only, Fiberglass Models Only 7A 1 5098300 Door Gasket, Inner and Outer, Stainless Models Only 7B 0.1 tube 1594800 Gasket Sealant, Stainless Steel Models Only, 3M #4799

8 1 5414000 Complete Door Assy., Fiberglass Outer

9 1 5098600 Complete Door Assy., Stainless Steel Outer

10A 1 5068601 Clamp, Door Plated Steel, Fiberglass Models 10B 1 5068600 Clamp, Door Stainless Steel Models

11 1 5064600 Door Adjustment Screw 12 1 5410000 Outer Acrylic Door Only

13A 1 5422300 Blower Assy., 115V Complete 13B 1 5422301 Blower Assy., 230V Complete 13C 1 5229100 Motorized Impeller ONLY 115V 13D 1 5229101 Motorized Impeller ONLY 230V

13E 1 1306600 Capacitor, 15 MFD-115V

13F 1 1306800 Capacitor, 4 MFD-230V 13G 4 1601800 Stud Mount Vibration Isolator 13H 1 5232700 Internal Harness Blower Assy. 14A 2 5225105 HEPA Filter 14B 2 5225106 ULPA Filter 14C 1 5241700 Bag-In-Bag-Out Kit 14D 12 1930200 Nut, Nylon #10-24

15 2 6962801 Airflow Silicone Tube Assy. 16 1 6962802 Main Chamber Pressure Tygon Tube Assy.

Window Components

Transfer Chamber Components

Blower and Filter Components

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Appendix A: Replacement Parts

Item Qty. Part Number Description

17A 1 1307000 Switch, 2 Position 17B 1 3823600 Retainer, Switch

18 1 5410600 Control Board, Airflow/Pressure Monitor 19A 1 1327201 Circuit Breaker, 10A 19B 1 1327200 Circuit Breaker, 3A, 115V 19C 2 1327205 Circuit Breaker, 2A, 230V

20 1 1333800 Inlet Power, IEC Connector 21A 1 1230700 Duplex Receptacle, Interior 100-115V, N. America 21B 1 1295200 Receptacle, UK 230V 21C 1 1295300 Receptacle, Schuko 230V 21D 1 1295100 Receptacle, China/Australia 230V 21E 1 1283900 Duplex Receptacle, Interior 230V, N. America

21F 1 5233400 Gray Duplex Wallplate, 100-115V or 23V, N. America 21G 1 1294900 Wallplate, UK or Schuko 21H 1 1295000 Cover, China/Australia 22A 1 1305800 Power Cord 100-115V, N. America 22B 1 1332600 Power Cord 230V, UK 22C 1 1336100 Power Cord 230V, Schuko 22D 1 1332700 Power Cord 230V, China/Australia 22E 1 1338000 Power Cord 230V, N. America

23 2 9721901 Lamp, Fluorescent F25T8-SP41, 25 watt 24 2 3836800 Lampholder, Push On

25 1 3838100 Ballast Assembly, Fluorescent 26A 1 5423000 Wiring Diagram, G.B. 115V 26B 1 5423100 Wiring Diagram, G.B. 230V 27A 1 5437400 Wiring Diagram, Doublewide 115V (Left Side) 27B 1 5437401 Wiring Diagram, Doublewide 230V (Left Side) 28A 1 3704400 Blower Speed Control, 115V 28B 1 3704401 Blower Speed Control, 230V (adjust potentiometer) 28C 1 1931500 Knob, Speed Control 28D 1 5229400 Extension, Speed Control 28E 1 1890302 Set Screw, Extension #8-32 x .12"

29A 1 5413900 Panel Access, Left 29B 8 1909217 Acorn Nut, #10-24

30 1 1934601 Heyco Bushing, Pass-Through 31A 1 5413601 Front Panel, Painted Fiberglass Models 31B 1 5413600 Front Panel, Stainless Steel, Stainless Steel Models 31C 2 1889308 Screw, #10-24 x 0.50 Phillips

32 1 5424700 Top Cover Assy.

Electrical Components

Miscellaneous

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Appendix A: Replacement Parts

Figure A-1

Replacement Parts

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DDiim

See the following for all dimensions of the glove boxes. For conversion, 1" equals 25.4 mm. All dimensions are in inches.

Product Service 1-800-522-7658

AAppppeennddiixx CC:: SSppeecciiffiiccaattiioonnss

This Appendix contains technical information about all the glove boxes including electrical specifications and environmental operating conditions.

• All models: 12 Amps, 100-115V or 230V, 50/60 Hz

• Single width units require one line cord and double width units require

two line cords.

Environmental Conditions

• Indoor use only.

• Maximum altitude: 6562 feet (2000 meters).

• Ambient temperature range: 41° to 120°F (5° to 49°C).

• Maximum relative humidity: 80% for temperatures up to 88°F (31°C),

decreasing linearly to 50% relative humidity at 104°F (40°C).

• Main supply voltage fluctuations not to exceed ±10% of the nominal

voltage.

• Transient over-voltages according to Installation Categories II (Over-

voltage Categories per IEC 1010). Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed.

• Used in an environment of Pollution degrees 2 (i.e., where normally

only non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.

Product Service 1-800-522-7658

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Static Electricity Test

The tests were performed by monitoring the level of static electricity found on the work surface and interior surfaces within the glove box. Static electricity levels were measured with an AlphaLab Surface DC Voltmeter

http://scientificmeter.com/surface_dc.htm.

A DESCO Emit Mini Zero Volt Ionizer http://www.descoemit.com/ViewProduct.aspx?pid=

50661&h=1323

was installed in the glove box. The DESCO ionizer is a dual steady state DC auto balancing bench top ionizer. The ionizer helps to neutralize static electricity on surrounding surfaces. The time required by the ionizer to neutralize static electricity will be the shortest when objects are within 12" to 36" directly in front of the ionizer. The time for neutralization increases as the distance from the ionizer increases. The time required for sufficient electrostatic decay will also vary depending upon user application, moisture levels within the supply air and the level of cleanliness within the glove box interior.

Product Service 1-800-522-7658

(

Electrostatic with Glove Box Blower & Ionizer Fan OFF 100 CFM Blower & Ionizer Fan ON After 1 Hour 100 CFM Blower & Ionizer Fan ON After 2 Hours

The mini zero volt ionizer’s neutralization time will be shortest when objects are approximately 12" to 36" directly in front of the ionizer and will increase as the distance from the ionizer increases. Set the fan speed switch on the side of the ionizer to the LOW or HIGH position. Higher airflow will result in faster neutralization rates. Position the ionizer so that the maximum airflow is directed at the items or area to be neutralized. The LED will turn on during power up and remain GREEN during normal operation. The ionizer has a grounding jack and must have a good earth ground to maintain proper balance. Always plug the AC power adaptor into the ionizer and then into the appropriate AC power source. The ionizer has no On/Off switch so it should be running as soon as it is plugged in. The ionizer is designed to run off of 24VDC ±10% 300mA.

The balance adjustment for the ionizer can be accomplished by inserting a small screwdriver or trimmer adjustment tool into the balance adjustment hole located on the side of the unit. To increase the output in a positive direction, turn the potentiometer in a clockwise direction. Conversely, to increase the output in a negative direction, turn the potentiometer in a counter clockwise direction. It is recommended that you use a surface DC voltmeter when adjusting the balance of the ionizer.

Under normal conditions, the ionizer will attract dirt and dust (especially on the emitter electrodes). To maintain optimum neutralization efficiency and operation, cleaning with isopropyl alcohol should be performed on a regular basis. If the performance of the ionizer degrades because of dirty or corroded points, the LED on the front of the unit will turn YELLOW; the audible alarm will sound continuously. Under this condition, the unit is not able to maintain balanced ionization. The input power cord must be disconnected before the unit is opened for maintenance. The emitter electrodes should be cleaned using the alcohol cleaners included or a swab wet with isopropyl alcohol. Unscrew the 4 screws on the back of the unit and then remove the screen. After cleaning the emitter electrodes, reinstall the screen and 4 screws. The emitter electrodes should not require replacement during the life of the unit with normal handling. Verify the balance of the ionizer with a surface DC voltmeter after cleaning.

Appendix D: Ionizer Fan Performance Criteria

Test Point Locations and Voltage Levels

Metal

Housing

Inlet

HEPA

+ 420 + 220 + 734 + 375 + 106 + 151 + 153

+ 96 + 114 + 290 + 93 + 45 + 52 + 35

+ 28 + 58 + 51 - 52 - 15 - 20 - 22

Metal Housing Exhaust

HEPA

Metal

Transfer

Chamber

Handle

Back Interior Surface

Left

FRP Interior Surface

Right

FRP Interior Surface

Static Electricity Test Results (VOLTS) on the Glove Box

Neutralization is best between +/-100V)

Center

FRP

Work

Surface

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AAppppeennddiixx EE:: CCoonnvveerrssiioonnss

Pressure Conversions

mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar

From

mmHg

in.Hg

in.H2O

ft.H2O

atm

lb/in.2

Kg/cm2

kPa

bar

1 .03937 .5353 .04461 .00132 .01934 .00136 .1333 .0013

25.40 1 13.60 1.133 .03342 .4912 .03453 3.387 .0339

1.868 .07355 1 .08333 .00246 .03612 .00254 .2490 .0025

22.42 .8826 12 1 .02950 .4334 .03048 2.988 .0299

760 29.92 406.8 33.90 1 14.70 1.033 101.3 1.013

51.71 2.036 27.69 2.307 .06805 1 .07031 6.895 .0689

735.6 28.96 393.7 32.81 .9678 14.22 1 98.05 .981

7.500 .2953 4.016 .3347 .00987 .1451 .0102 1 .01

750 29.53 401.6 33.47 .987 14.51 1.02 100 1

Flow Rate Conversions

From

lit/sec

gal/min

ft3/sec

ft3/min

bbl/hr

bbl/day

lit/sec gal/min ft3/sec ft3/min bbl/hr bbl/day

1 15.85 0.03532 2.119 22.66 543.8

0.06309 1 0.00223 0.1337 1.429 34.30

28.32 448.8 1 60 641.1 1.54x10

0.4719 7.481 0.01667 1 10.69 256.5

0.04415 0.6997 0.00156 0.09359 1 24

0.00184 0.02917 6.50x10

0.00390 0.04167 1

Product Service 1-800-522-7658

AAppppeennddiixx FF:: RReeffeerreenncceess

Many excellent reference texts and booklets are currently available. The following is a brief listing:

Pharmaceutical Isolators, A Guide to their application design and control. Pharmaceutical Press 2004, Editors: Midcalf, Phillips, Neiger, and Coles.

Isolation Technology, A Practical Guide, 2004 CRC Press, 2nd Edition, www.crcpress.com

International Standard ISO 10648-2 Containment Enclosures

• Classification according to leak tightness and associated checking

methods.

International Standard ISO 14644-7 Clean Rooms and associated controlled environments

• Separate device such as glove boxes and isolators.

NIOSH Pocket Guide to Chemical Hazards U.S. Dept of Health and Human Resources Centers for Disease Control and Prevention National Institute for Occupational Safety and Health

Product Service 1-800-522-7658

Labconco Protector Filtered Glove Box User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual