Labconco Protector Controlled Atmosphere Glove Box User Manual

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User’s Manual

Protector®Controlled Atmosphere Glove Boxes

Base Catalog

Model Number

50600 Manual Fiberglass Single 10 31 35 40 45

50800 Auto Fiberglass Single 10 31 35 40 45

50800 Combination Fiberglass Single 60 65 70 75 80

Notes: Manual is basic pressure control.

Auto is automatic pressure control. Combination is automatic pressure control with blower and filter conversion for particulates.

Operation

Type

Liner

Material

Steel

America

100-115V

50/60 Hz

Liner

Width

Single 10 31 35 40 45 50601 Manual Stainless

Double 12 33 37 42 47

Single 10 31 35 40 45 50801 Auto Stainless

Double 12 33 37 42 47

Single 60 65 70 75 80 50801 Combination Stainless

Double 62 67 72 77 82

(Last Two Digits of Configured Catalog Number)

North

Electrical Receptacle Voltage/Frequency

British

(UK) 230V

50/60 Hz

Schuko

230V

50/60 Hz

China

Australia

230V

50/60 Hz

America

60 Hz Only

North

230V

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Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com

Please read the User’s Manual before operating the equipment.

The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of defective material and workmanship, provided such defect results from normal and proper use of the equipment.

The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner, except the following;

• Purifier® Logic® Biological Safety Cabinets and PuriCare® Lab Animal Research Stations

carry a three-year warranty from date of installation or four years from date of shipment from Labconco, whichever is sooner.

• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date

of installation or three years from date of shipment from Labconco, whichever is sooner.

• Blood Drawing Chairs carry a ten year warranty.

• Carts carry a lifetime warranty.

• Glassware is not warranted from breakage when dropped or mishandled.

This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This statement may be altered by a specific published amendment. No individual has authorization to alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or accidental breakage is not covered.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.

Part #5426400, Rev. -

ECO F874

AABBLLEE

CHAPTER 1: INTRODUCTION 1

CHAPTER 2: PREREQUISITES 7

Support, Vibration & Preventive Requirements 8 Location Requirements 8 Oil & Exhaust Requirements for the Pressure Relief Bubbler 8 Electrical Requirements 9 Plumbing Requirements for Inert Gas, Vacuum, & Gas Purifier/

Drying Train 9

Space Requirements 10

CHAPTER 3: GETTING STARTED 11 Unpacking the Glove Box 12 Installing the Glove Box on a Supporting Structure &

Work Surface 12 Adding the Oil & Connecting the Pressure Relief Bubbler to

Outside Exhaust 13 Installation of Gloves to the Glove Ports 16 Connecting the Electrical Supply Source to the Glove Box 16 Connect the Plumbing Lines for Gas, Vacuum &

Gas Purifiers/Drying Trains 17 Validating the Glove Box 21 Oxygen Method Leak Testing Summary (ISO 10648-2) 24 Pressure Change Method Leak Testing Summary (ISO 10648-2) 25

CHAPTER 4: PERFORMANCE FEATURES AND

Performance Features 26 Safety Precautions 30

CHAPTER 5: USING YOUR GLOVE BOX 32 Routine Daily Work Procedures 32

Hazardous Use with Chemicals 34 Prohibited Acid Use 34 Auto Pressure Controller Operation 34 Front Panel Description 35 Rear Panel Description 35 Main Modes of Operation 36 Glove Box Pressure Control – Operation Mode 1 37 Glove Box Evacuation (Purge)/Fill – Operation Mode 2 38

SAFETY PRECAUTIONS 26

OONNTTEENNTTSS

Transfer Chamber Evacuation (Purge) and Fill – Operation Mode 3 39 Setup Menu – Operation Mode 4 40

CHAPTER 6: MAINTAINING YOUR GLOVE BOX 45 Routine Maintenance Schedule 45 Initial Certification 46 Recertification 46 Fluorescent Light Replacement 46 Solenoid Valve Replacement 48 Auto Pressure Controller Circuit Board Replacement 48

CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 50

CHAPTER 8: TROUBLESHOOTING 57

APPENDIX A: REPLACEMENT PARTS 58

APPENDIX B: DIMENSIONS 61

APPENDIX C: SPECIFICATIONS 62

APPENDIX D: CONTROLLED ATMOSPHERE OXYGEN &

MOISTURE REMOVAL RATES 63

APPENDIX E: APPROXIMATE LIFE SPAN FOR ACCESSORY ATMOSPURE RE-GEN GAS PURIFIER 69

APPENDIX F: IONIZER FAN PERFORMANCE CRITERIA 70

APPENDIX G: HELIUM LEAK TEST PROCEDURE 72

APPENDIX H: CONVERSIONS 74

APPENDIX I: REFERENCES 75

DECLARATION OF CONFORMITY 76

CChhaapptteerr 11:: IInnttrroodduuccttiioonn

Congratulations on your purchase of a Labconco Protector® Controlled Atmosphere Glove Box manufactured with fiberglass or stainless steel in a single or double width configuration. The Protector Controlled Atmosphere Glove Box comes equipped with four manual valves, pressure relief bubbler and either analog chamber pressure gauge indicators, or digital pressure gauge indicators. The Protector Controlled Atmosphere Glove Box is manufactured with either manual or automatic pressure control in single or double width. Combination glove boxes utilize automatic pressure control and feature HEPA or ULPA filters with integral blower. See Figures 1-1, 1-2, 1-3, and 1-4 at the end of Chapter 1. The glove boxes are designed to provide an effective physical barrier between the laboratory and the glove box interior. The barrier provides three distinct advantages. First, it helps protect the technician from hazardous materials. Second, it protects materials and laboratory equipment inside the main chamber from the effects of exposure to ambient air, oxygen, and moisture levels. Third, it provides a protective, leak-tight environment for your research, testing, or production procedures.

The permeation rate for stainless steel glove boxes is less than 0.16 ppm/min as tested to ISO 10648-2 test methods. The permeation rate for fiberglass glove boxes is less than 0.30 ppm/min as tested to ISO 10648-2 test methods. Each Protector Glove Box is helium leak tested at the factory to ensure no individual leaks greater than 1 x 10

Product Service 1-800-522-7658

-6

cc/sec. The test results are as follows:

Chapter 1: Introduction

Glove Box Type

Attainable

Purity

(ppm)

Oxygen Permeation at -4" w.g. per

ISO 10648-2 Test Method (ppm/min)

ISO 10648-2 Acceptance Level

for Class 1 Controlled Atmosphere Glove Box (ppm/min)

Usable Permeation

with pressure from (-1" w.g. to +1" w.g.)

(ppm/min)

Gas Port

Permeant

Glove

Covers

Installed 0.4 0.2 Installed 2 1

None 0.9 0.8 None 3 2

None 0.30 0.16

None <1.67 <1.67

None 0.16 0.14 None 0.17 0.10

Protector (FRP) Protector (stainless)

The Protector Glove Boxes offer many unique features to enhance performance. To take full advantage of them, you must read and understand this manual and keep it handy for future reference. If you are unfamiliar with how the Glove Boxes operate, please review Chapter 4: Performance Features and Safety Precautions before you begin working in the glove box. Even if you are an experienced user, please review Chapter 5: Using Your Glove Box so you can use the glove box efficiently.

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Chapter 1: Introduction

Protector Controlled Atmosphere Glove Box with Auto Pressure Control shown with AtmosPure Re-Gen Gas Purifier and Adjustable Height Base Stand

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Figure 1-1

Chapter 1: Introduction

Protector Controlled Atmosphere Glove Box with Manual Pressure Control and Adjustable Height Base Stand

Figure 1-2

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Chapter 1: Introduction

Protector Combination Glove Box utilizes Auto Pressure Control for controlled atmospheres and filtered air with Integral Blower for particulate containment and cleanliness applications. The Glove Box is shown with the Adjustable Height Base Stand.

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Figure 1-3

Chapter 1: Introduction

Protector Double Width Controlled Atmosphere Glove Box with Auto Pressure Control shown with AtmosPure Re-Gen Gas Purifier mounted on a doublewide mobile base stand

Figure 1-4

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CChhaapptteerr 22:: PPrreerreeqquuiissiitteess

Before you install the glove box, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the glove box. Additionally, the glove box should be strategically placed in the lab to provide efficient workflow and prevent operator interference from normal traffic flow.

Carefully read this chapter to learn the requirements for your installation site:

• The support, vibration and preventive requirements.

• The location requirements.

• The oil requirements and exhaust requirements for the pressure relief

bubbler.

• The electrical power requirements.

• The plumbing requirements for gas, vacuum, and gas purifiers or

drying trains.

• The space requirements.

Refer to Appendix B: Protector Glove Box Dimensions for complete glove box dimensions.

Refer to Appendix C: Protector Glove Box Specifications for complete electrical and environmental conditions, specifications and requirements.

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Chapter 2: Prerequisites

Support, Vibration and Preventive Requirements

In the preparation of a glove box site, please consider the following:

• A bench or stand that is rigidly mounted to the floor or fixed to the wall,

but not both, may be appropriate. 35" to 40" (889mm to 1,016mm) is typical for standing height (Labconco adjustable height base stands offered in Chapter 7 vary from 33" to 40" or 838mm to 1,016mm).

• The corners of a building typically have less vibration than the center,

which promotes analytical balance stability.

• The bench typically should not contain any vibration-producing

equipment, such as shakers or pumps.

• A marble slab with dampening pads placed within the enclosure is an

effective low cost means of controlling vibration for analytical balance operation (see Balance Vibration Isolator in Chapter 7: Accessorizing Your Glove Box).

Location Requirements

The Protector Glove Boxes have been designed to rest on a typical 29"-30" (737mm-762mm) deep work surface. The height should be 35"-40" for standing position. Avoid placing the glove box in high traffic areas where walking might disrupt the operator or experimentation.

Oil and Exhaust Requirements for the Pressure Relief Bubbler

To prevent over or under pressurization of the main glove box chamber and prevent glass breakage, the Protector Controlled Atmosphere Glove Box includes a pressure relief bubbler that activates at pressures and vacuums above +5" water gauge or below -5" water gauge. The glove box ships with an oil container with a specific gravity of 0.87 and the bubbler must be filled with oil to a level of 6" (152mm). See Chapter 3 for instructions for adding oil.

WARNING: The overpressurization of air at positive pressure would allow any contaminants inside the glove box

to vent into the room. To control this venting into the laboratory, Labconco recommends exhausting the 3/4" diameter pipe from the pressure relief bubbler to the outside. If needed, 3/4" I.D. neoprene hose circulation kits can be ordered from Chapter 7. If needed, purchase the 5441403 HEPA Filter Exhaust Kit from Chapter 7 to exhaust the bubbler through HEPA filtration and into the room.

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Chapter 2: Prerequisites

WARNING: In the case of failure caused by excessive vacuum within the main chamber, the user must safely

determine whether laboratory atmosphere entering the glove box could result in fire, explosive hazard or damage to property inside the glove box.

Electrical Requirements

Electrical receptacles should be located nearby for connecting the glove box cords, or other optional laboratory equipment. Single width units require two line cords and double width units require three line cords. Line cords are each rated at 100-115V, 12A or 230V, 12A. The entire glove box is rated 115V, 12A or 230V, 12A or 100V, 12A. One outlet is located inside the glove box and is rated for 115V, 10A or 230V, 10A or 100V, 10A. The exterior vacuum pump outlet is rated 100-115V, 12A or 230V, 6A. Doublewide models have an additional outlet, located inside the center of the glove box and is rated for 115V, 10A or 230V, 10A or 100V, 10A. Additional power strip accessories are available and can be connected to the interior receptacle outlet (see Chapter 7).

Plumbing Requirements for Inert Gas, Vacuum, and Gas Purifier/Drying Train

The Protector Controlled Atmosphere Glove Box has two valves located on the main glove box chamber and two valves located on the transfer chamber. The two valves on the main chamber should be used for the operation of the main chamber inert gas inlet and vacuum outlet lines. For effective operation and to prevent overpressurization that can cause glass breakage, the gas inlet lines must never be set at a pressure greater than 10-14 psi (69-96 kPa) through the use of a gas supply pressure regulator. The two valves on the transfer chamber are used for inert gas inlet and vacuum outlet lines. The transfer chamber lines can be connected together with the main chamber gas inlet and vacuum outlet lines to simplify gas and vacuum connections to both chambers. On the rear of the Protector Glove Boxes, there are two 3/4" compression fittings for connection to an accessory AtmosPure Re-Gen Gas Purifier or other drying train. The Gas Purifier per Figure 1-1 in Chapter 1 is used for the removal of oxygen and moisture from the main chamber atmosphere. Other drying trains can be used to remove organic vapors, acids, ammonia, or radioisotopes. See Chapter 7 for ordering information and Chapter 3 for setup. Illustrations for gas, vacuum, and gas removal accessories are shown in Chapter 3.

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Chapter 2: Prerequisites

All solenoid valves utilize 3/8" (9.5mm) compression fittings or 3/8" (9.5mm) hose barbs for soft tubing. Both styles are offered for your convenience. Accessory gas and vacuum tubing kits are described in Chapter

7. Should you later require automatic chamber pressure controls (only for manually operated Protector Controlled Atmosphere Glove Boxes), see Chapter 3 for details on the accessory Pressure Controller and Chapter 7 for ordering information. The Pressure Controller is standard with Protector Glove Boxes with Auto Pressure Control and it automatically monitors and controls pressures in both the main chamber and transfer chamber; it also automates the operation of evacuating/purging and filling of the main chamber (gas outlet and gas inlet) up to 499 cycles and transfer chamber up to 199 cycles. Consult your Safety Officer should you require ventilation and/or filtration of the vacuum pump exhaust. Filtered exhaust (0.2 micron) and contaminate removal traps are available for the vacuum pump (see Chapter 7). See Chapter 3 for typical installation of vacuum outlet and inert gas inlet lines.

Space Requirements

The dimensions are shown in Appendix B: Dimensions. Adequate space to the right of the transfer chamber is required for loading and unloading.

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CChhaapptteerr 33:: GGeettttiinngg SSttaarrtteedd

Once the site for your glove box is properly prepared, you are ready to unpack, inspect, install, and validate performance of your system. Read this chapter to learn how to:

• Unpack and move the glove box.

• Set up the glove box with the proper supporting structure and work

surface.

• Add the oil and if required connect an exhaust system to the pressure

relief bubbler.

• Install the gloves.

• Connect the electrical supply.

• Connect the plumbing lines for gas, vacuum, and gas purifiers or

drying trains.

• Validate performance of the glove box.

Each Protector Glove Box model weighs as follows:

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• Fiberglass, 350 lbs/157 kg.

• Stainless, 500 lbs/225 kg.

• Stainless Doublewide, 1000 lbs/450 kg.

The shipping container allows for lifting with a mechanical lift truck or floor jack. If you must lift the glove box manually, follow safe-lifting guidelines. Do not lift by the upper sheet metal façade and front panel as damage can occur to the glove box liner.

Chapter 3: Getting Started

Unpacking the Glove Box

We recommend that you do not remove the glove box from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack.

Carefully remove the shrink-wrap and crating on the glove box and inspect it for damage that may have occurred in transit. If damaged, notify the

delivery carrier immediately and retain the entire shipment intact for inspection by the carrier.

THE UNITED STATES INTERSTATE COMMERCE COMMISSION RULES REQUIRE THAT CLAIMS BE

)

FILED WITH THE DELIVERY CARRIER WITHIN FIFTEEN (15) DAYS OF DELIVERY.

DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.

IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.

Do not discard the packing material until you have checked all of the components and tested the glove box.

Installing the Glove Box on a Supporting Structure and Work Surface

Exercise caution when lifting or moving the glove box.

When installing the glove box onto a work surface or benchtop, ensure that the structure can safely support the combined weight of the glove box and any related equipment. The work surface should be as wide as the entire glove box to properly support it. The front of the glove box should be aligned with the front of the work surface for optimal comfort. A height of 35"-40" (889 mm-1016 mm) is appropriate for standing operation. Labconco base stands from Chapter 7 may be adjusted in height from 33"-40" (838 mm

-1016 mm).

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Chapter 3: Getting Started

Adding the Oil and Connecting the Pressure Relief Bubbler to Outside Exhaust

The glove box ships with a separate oil container with a specific gravity of

0.87. The pressure relief bubbler prevents over or under pressurization of the glove box main chamber and activates at pressures and vacuums above +5" water gauge (1245 Pa) or below -5" water gauge (-1245 Pa) to prevent glass breakage and glove box liner damage. See Figure 3-1 as the bubbler must be filled with oil to a level of 6" (152 mm) measured vertically from the bottom of the stainless steel bubbler. Add the oil through the open exhaust port using a 3/4" I.D. hose (#5443500) and funnel.

As stated in Chapter 2, the main chamber pressure relief bubbler can be exhausted back into the room or exhausted to the outside, if required. Figure 3-1 shows the location of the pressure relief bubbler exhaust. After consulting with your Safety Officer and reading the prerequisites in Chapter 2, connect the exhaust the bubbler to the outside if your application requires it. If needed, the 3/4" I.D. neoprene circulation hose kit can be used and ordered from Chapter 7 to exhaust the bubbler to the outside. Furthermore, should your application require HEPA filtered exhaust to the room, purchase and install the 5441403 HEPA Filter Exhaust Kit from Chapter 7.

WARNING: The weight of the pressure relief exhaust

ductwork system must be supported independently of the glove box superstructure or damage may occur.

The exhaust system should be installed by a qualified HVAC contractor.

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Chapter 3: Getting Started

Liquid level sight gauge. Fill oil to 6" (152 mm) from bottom.

Consult with Safety Officer for exhausting 3/4" OD to the outside.

Fill with 0.87 specific gravity oil using a 3/4" I.D. hose and funnel.

Bottom of bubbler

Figure 3-1

Pressure Relief Bubbler Set-up

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Chapter 3: Getting Started

ON/OFF switch number one

Input power cord number one

Exterior vacuum pump receptacle (230V shown)

C.A. COM Port. 6-Pin Communication Cable Connector for shared vacuum pump use with accessory AtmosPure Re-Gen Gas Purifier (auto pressure control models only)

Foot switch connection (auto pressure control models only)

Plug vacuum pump in here for shared vacuum pump use.

Input power cord number two

ON/OFF switch number two

Electrical Connections, Back of Glove Box with Auto Pressure Control

Figure 3-2

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Chapter 3: Getting Started

Installation of Gloves to the Glove Ports

With the thumbs up and in a right/left orientation, secure the gloves in place on the glove ports by stretching the beaded glove cuff into the glove port groove nearest the window. Install the separate 8" diameter O-ring over the gloves, into the outer groove of the glove port surface. Stainless steel band clamps are provided for securing the separate O-ring into the glove port groove. Replacement gloves and parts are listed in Chapter 7.

Figure 3-3

Glove Installation

Connecting the Electrical Supply Source to the Glove Box

Connect the power cords supplied to both IEC electrical supply plugs located on the back of the glove box. For doublewide models, connect one extra power cord for the centrally located duplex on the left side. 100-115V models are rated at 12 amps for each power cord. 230V models are rated at 12 amps for one power cord and 6 amps for the other power cord to power the exterior vacuum pump outlet. The maximum circuit load for the interior electrical duplex is 10 amps. The exterior vacuum pump receptacle is rated at 8 amps for 100-115V models and 4.5 amps for 230V models. On auto pressure control and combination models, a foot switch for pressure control is supplied and is connected (3 position connector) to the back of the glove box as marked. See Figure 3-2 for all electrical connections. See Figures 1-1 and 1-3 for foot switch.

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Chapter 3: Getting Started

Connect the Plumbing Lines for Gas, Vacuum, and Gas Purifiers/Drying Trains

Labconco offers various vacuum and gas tubing installation kits listed in Chapter 7 for connecting inert gas, vacuum, and gas purifiers to the glove box. Refer to Figures 3-4, 3-5, 3-6, 3-7, and 3-8 that depict the installation tubing kits. From Chapter 7, order Gas and Vacuum Tubing Connection Kit 5245100 or 5245200 and install using Figures 3-4 through 3-8. For effective

operation and to prevent overpressurization that can cause glass breakage, the inert gas line must never be set at a pressure greater than 10-14 psi (69-96 kPa) through the use of a gas supply pressure regulator.

The primary connecting method involves the use of 3/8" O.D. x 3/16" I.D. tubing for gas and vacuum line connections; a tube support insert must be placed inside the soft tubing to prevent the tube from disconnecting. The alternative connecting method uses a tube connector and hose clamp instead of a tube support insert. Rotary vane vacuum pump model numbers can be found in Chapter 7 and are required for all models as depicted in Figure 3-7.

Consult your Safety Officer to determine the suitability and requirement for venting the exhaust port of a vacuum pump to the outside. An

accessory 0.2 micron filter or vacuum line particulate trap can be installed in the vacuum line to prevent particulate material contamination of the vacuum pump. The 0.2 micron filter can also be installed in the inert gas supply line connected to the main chamber and transfer chamber to insure a particulate free gas supply (see Chapter 7). If required, other accessory chemical traps can also be added to the vacuum line to prevent vacuum pump contamination (see Chapter 7).

For ultra low oxygen and moisture purity levels, Labconco recommends the accessory AtmosPure Re-Gen Gas Purifier shown in Figure 1-1, Figure 1-3, and Figure 3-7. (See performance results listed in Chapter 1.) See Chapter 7 to order the AtmosPure Re-Gen Gas Purifier. Chapter 7 and Figure 3-7 also list and depict the AtmosPure Regeneration Plumbing Kit and AtmosPure Circulation Kit required for proper performance of the AtmosPure Re-Gen Gas Purifier. Chapter 7 also lists alternative drying trains that reduce oxygen and moisture. Contact Labconco for further details.

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Chapter 3: Getting Started

Rotary vane vacuum pump electrical connection

Accessory AtmosPure ReGen Gas Purifier Circulation Connections (3/4" compression fittings)

Electronic solenoid valves for glove box models with auto pressure control

Inert gas connections (3/8" compression)

Vacuum line connections (3/8" compression)

Figure 3-4

Required Plumbing Connections

– Rear view of glove box with auto pressure control

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Chapter 3: Getting Started

(only used with flexible lines for soft tube support during compression)

Figure 3-5

Typical Orientation of Ferrules

Alternate Tube Connection Using Hose Connector

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Figure 3-6

Chapter 3: Getting Started

Figure 3-7

Rear View of Glove Box with auto pressure control, all plumbing connections,

and accessory AtmosPure Re-Gen Gas Purifier with plumbing connections

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Chapter 3: Getting Started

Figure 3-8

Rear View of Glove Box with manual control valves and the

plumbing connections

Validating the Glove Box

Each Protector Glove Box has been helium leak tested at the factory for integrity and found to have no leaks greater than 1 x 10 positive pressure of 5 inches helium. Refer to Chapter 6, Initial Certification and Appendix G for Helium Leak Test Method.

Overall Glove Box Main Chamber Leak Test The Protector Controlled Atmosphere Glove Box has been tested with an oxygen monitor detecting oxygen levels as low as 0.1 ppm; the rate of increase in oxygen over time was determined in accordance with the ISO 10648-2 oxygen leak decay test method. The results validate that the Protector Controlled Atmosphere Glove Box meets Class 1 conditions for controlled atmosphere chambers. Figure 3-9 and 3-10 show test results of the oxygen permeation or oxygen level increase as a function of time. Per ISO specifications, Class 1 controlled atmosphere chambers have a specified hourly leak rate of less than 5 x 10

-4

(< 1.67 ppm/min) per Table 3-1 listed at the end

of Chapter 3 with performance results summarized in Chapter 1.

-6

cc/sec while at a

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Chapter 3: Getting Started

Protector Stainless CA Glove Box

Oxygen Permeation at -4" H2O

ISO 10648-2 Test Method

Barometric Pressure

29.81 inches of Hg

Time Minutes

012345678910

Oxygen ppm

Figure 3-9

Main Chamber

Pressure: -4 Inches

Transfer Chamber

Pressure: 0 inches of

Leak Rate Box

Class 1 < 0.0005

Permeation

0.16ppm/min

4.7x10¯

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120

Chapter 3: Getting Started

Protector Fiberglass CA Glove Box

Oxygen Permeation at -4" H2O

ISO 10648-2 Test Method

100

Time Minutes

0 5 10 15 20 25 30 35 40

Oxygen ppm

Figure 3-10

The validation requirements and required testing instruments for all applications shall be determined by the end user and Safety Officer. Other validation test methods such as pressure leak decay tests may be conducted per ISO 10648-2 standards. The pressure leak decay test may be performed, but the pressure inside the glove box must be adjusted accurately

for temperature

and barometric pressure.

These pressure leak decay tests are conducted under negative pressure of

-250 Pa (-1" water gauge) for operational use or -1000 Pa (-4" water gauge) for the acceptance test; the pressure leak decay tests are usually performed under stable barometric pressure and temperature conditions to minimize the effect of these variables.

A 0.1°C fall in temperature results in an equivalent pressure change of -34 Pa or -0.14 inches of water gauge. A 0.01 inches of mercury rise in barometric pressure results in an equivalent pressure change of -34 Pa or

-0.14 inches of water gauge.

Barometric Pressure

29.61 inches of Hg

Main Chamber

Pressure: -4 Inches

Transfer Chamber

Pressure: 0 inches of

Leak Rate Box

Class 1 < 0.0005

Permeation

0.30ppm/min

8.7x10¯

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Chapter 3: Getting Started

Table 3-1

Classification of Glove Box Containment per ISO 10648-2

Class Hourly Leak Rate (Tf) Usage Example

1 ≤ 5 x 10-4 hr-1 Controlled atmosphere with high inert gas purity conditions 2 < 2.5 x 10-3 hr-1 Controlled atmosphere under inert gas conditions or

permanently hazardous atmosphere 3 < 10-2 hr-1 Permanently hazardous atmosphere 4 < 10-1 hr-1 Atmosphere which could be hazardous

The required classification of leak tightness of each glove box must be decided by the end user, their Safety Officer, and any oversight authorities.

Oxygen Method Leak Testing Summary (ISO 10648-2)

The method consists of measuring the increase in the oxygen concentration as a function of time inside the glove box main chamber, previously purged with an inert gas. The difference in the oxygen concentration between the end and the beginning of the test, calculated on an hourly basis, gives the hourly leak rate Tf:

Tf = 300 * (O2f –O2i) / (t * 106) where O2f is the final oxygen concentration (ppm). O2i is the initial oxygen concentration (ppm). t is the duration of the test in minutes. 300 = 60 x 100/20 where 60 represents 60 minutes in an hour and 100/20 represents 20% oxygen in normal air.

Note: Use negative pressure of 1000 Pa (-4" water gauge) for acceptance test and negative pressure of 250 Pa (-1" water gauge) for operational test.

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Chapter 3: Getting Started

Pressure Change Method Leak Testing Summary (ISO-10648-2)

The method consists of measuring the pressure rise per unit time after establishing a negative pressure in the main chamber of the glove box. Use

-1000 Pa for the acceptance test and -250 Pa for the operational test. The hourly leak rate includes adjustments for barometric pressure and temperature changes which can be calculated as T

Tf = (60/t) * ((PnT1/P1Tn) -1) where t is the duration of the test in minutes. P1 is the absolute pressure (ambient barometric pressure minus under pressure) at the first reading in Pascals. Pn is the absolute pressure (ambient barometric pressure minus under pressure) at the last reading in Pascals. T1 is the temperature at the first reading in Kelvin. Tn is the temperature at the last reading in Kelvin. 60 represents 60 minutes in an hour. T in Kelvin is equal to degrees Celsius + 273.

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SSaaffeettyy PPrreeccaauuttiioonnss

Performance Features

The Protector Controlled Atmosphere Glove Box comes fully equipped with four manual valves, pressure relief bubbler, and pressure monitoring of the main chamber and transfer chamber. Manual Control models utilize analog gauges and Auto Pressure Controller models utilize automatic control with digital LCD display gauges. Protector Controlled Atmosphere Glove Boxes are available in single width fiberglass, single width stainless steel, and double width stainless steel. The Protector Controlled Atmosphere Glove Box is designed to meet the needs of the laboratory scientists requiring an inert atmosphere for sensitive chemical operations. Labconco has engineered the Protector Controlled Atmosphere Glove Boxes to meet the ISO 10648-2 Class 1 atmosphere leak specification. The Protector Controlled Atmosphere Glove Box protects the technician from hazardous materials and protects valuable laboratory materials from the effects of oxygen and moisture exposure. The Protector Controlled Atmosphere Glove Boxes provide a leak tight environment for manufacturing and chemical research procedures. See Figure 4-1 to refer to a detailed description of the Protector Controlled Atmosphere Glove Box main performance features.

1. Fiberglass-lined models include:

• One piece molded fiberglass liner with clear acrylic inner and outer

transfer chamber doors.

• Performance tested to maintain oxygen levels as low as 0.4 ppm and

moisture levels as low as 2 ppm.

• Oxygen permeation <0.3 ppm/min per ISO 10648-2. Exceeds Class 1

conditions at <1.67 ppm/min.

• Illumination exceeds 120 foot candles.

2. Stainless steel-lined models include:

• Type 304 stainless steel liner with Type 304 stainless steel inner and

outer transfer chamber doors. Type 304 stainless steel front panel.

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Chapter 4: Performance Features and Safety Precautions

• Performance tested to maintain oxygen levels as low as 0.2 ppm and

moisture levels as low as 1 ppm.

• Oxygen permeation <0.16 ppm/min per ISO 10648-2. Exceeds Class

1 conditions at <1.67 ppm/min.

• Illumination exceeds 80 foot candles.

• Doublewide models include 4 glove ports with main chamber volume

of 34 cu. ft.

3. Basic Manual Control Models include:

• Main chamber pressure analog gauge with readings from -5 to +5

inches of water gauge.

• Transfer chamber pressure analog gauge with readings from 0 to -29.5

inches of mercury.

• Switch to control exterior outlet for vacuum pump connection.

4. Auto Pressure Controller Model include:

• Automatic control of the main chamber pressure (-5 to +5 inches of

water gauge), main chamber evacuation and filling cycles (up to 499 cycles), and transfer chamber evacuation and filling cycles (up to 199 cycles).

• Regulation of vacuum/pressure between -5 and +5 inches of water

gauge in the main chamber and between 0 and -29.5 inches of mercury in the transfer chamber.

• Mutable, audible alarm that sounds when pressure/vacuum level is

outside set limits.

• LCD display of main chamber and transfer chamber pressure in

inches of water, inches of mercury and mBar.

• Three-position foot pedal (increase gas/fill, off, evacuation/decrease

gas).

• C.A. COM (6-Pin) port for shared vacuum pump use with the optional

AtmosPure™ Re-Gen Air Purifier.

All models feature:

5. Large viewing area, 36" w x 27.6" high, provides maximum visibility.

Removable viewing window is 3/8" thick, laminated safety glass. Double wide models have two viewing areas.

6. Pair of neoprene gloves, 0.015" thick, 30" long, size 9 ¾. Doublewide

models include two pair. See Replacement Parts for all glove types and sizes.

7. Space-saving inner and outer transfer chamber doors that pivot upward,

counterbalanced and equipped with quick-latches.

8. Four manual valves – two on the main chamber and two on the transfer

chamber – for evacuating, filling and manual control. With 3/8" compression fittings and 3/8" hose barbs.

9. Factory-installed, rear-mounted, low profile stainless steel pressure relief

bubbler at ±6 inches of water gauge.

10. Two rear-mounted inlet and outlet 3/4" OD compression fittings for easy

connection to optional AtmosPure Re-Gen Air Purifier or other accessory drying train.

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Chapter 4: Performance Features and Safety Precautions

11. Interior right-hand side wall-mounted electrical duplex receptacle with 10

amp maximum (100/115 volt models) or 10 amp maximum (230 volt models) circuit load. Doublewide models include an extra interior receptacle.

12. Interior left-hand side wall-mounted pass-through plug for on-site

installation of an optional accessory; electrical outlet, RS-232 port, gas/liquid port, or port for oxygen or moisture monitor.

13. Switches for light and electrical duplex.

14. Two 8" ID, epoxy-coated aluminum, double-grooved glove ports

mounted 17" apart, with neoprene gaskets. Doublewide models have four glove ports.

15. Two 25-watt fluorescent lights. Doublewide models feature four 25-watt

fluorescent lights.

Combination models include:

16. Combination models include all the features of the Protector Controlled

Atmosphere Gove Box with Auto Pressure Control as well as all the features of Protector Filtered Glove Boxes. Through the use of manually operated valves suspended from the ceiling of the main chamber, the auto pressure control panel, and the manually operated valves on the transfer chamber, the Protector Combination Glove Box can easily be converted from a Protector Controlled Atmosphere Glove Box to a Protector Filtered Glove Box. See Figure 1-3 in Chapter 1. Consult the Protector Filtered Glove Box manual for details concerning the blower and filtration system.

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Chapter 4: Performance Features and Safety Precautions

Performance Features

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Figure 4-1

Chapter 4: Performance Features and Safety Precautions

Safety Precautions

1. It is the responsibility of the user to determine the suitability of this

product for the intended applications. Consult your Safety Officer for application review.

2. Although the glove box has been engineered to maintain optimum

operator safety, caution should always be used while working. Prior to using the glove box, check to make sure that the main chamber pressure is set for comfortable glove manipulation. Changes in glove position will affect pressure in the glove box.

3. Use good housekeeping in the glove box at all times. Clean up spills

immediately. Periodically clean interior per your standard operating procedures for the specific chemicals in use.

4. This product is NOT designed or intended to be explosion proof.

It is the responsibility of the user to determine and avoid the lower explosive limits and flammability of the enclosed gases used within the controlled atmosphere glove box. The user is responsible for taking proper precautions to prevent equipment damage or injury due to an explosion or combustion of chemicals.

5. The use of flammable gases or solvents in the glove box should be

limited. Care must be taken to ensure against the concentration buildup of flammable or explosive gases. DANGER: The addition

of oxygen inside the glove box must be avoided to prevent explosion, death, or serious injury.

6. Perchloric acid use in this glove box is prohibited.

7. Radioisotope material usage in the glove box should be approved by

your Safety Officer.

8. A qualified certification technician should test the glove box before it

is initially used.

9. The glove box should be recertified at least annually after installation.

10. The use of safety goggles, protective clothing, and any other personal

protective equipment recommended by your Safety Officer should be observed.

11. The pressure relief bubbler provides protection from over or

under pressurization and should be filtered and/or ducted to the outside if working with hazardous materials. Consult your Safety

Officer. When operating the transfer chamber gas inlet and vacuum outlet valves, insure the inner transfer door is closed. Failure to do so can result in the pressure relief valve opening, thereby, introducing laboratory air into the glove box main chamber or glove box air into the laboratory. All filters and ducting are NOT included with the glove box.

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Chapter 4: Performance Features and Safety Precautions

12. All powders, gases, and particulates removed by the operation of

the vacuum pumps connected to this glove box are to be filtered and/or ducted to the outside.

13. Exhaust vacuum pumps should be filtered and/or ducted to the

outside when using hazardous materials. Consult your Safety

Officer. Manipulations that generate gases or vapors from toxic chemicals or radionuclides, must be evaluated carefully from the standpoint of buildup to dangerous levels. The decontamination of the glove box and compliance with applicable regulations is the responsibility of the operator and safety officer. Venting or filtering of vacuum pump exhaust is the operator’s responsibility.

14. Ensure that the glove box is connected to electrical service in

accordance with local and national electrical codes. Failure to do so may create a fire or electrical hazard. Do not remove or service any electrical components without first disconnecting the glove box from electrical service.

15. Ensure only trained operators use the glove box. New users must

review the User’s Manual and become familiar with the operation of the glove box.

16. Tag the glove box with appropriate warnings, if work inside the glove

box is hazardous or needs to remain untouched. Tag the glove box if it requires servicing.

17. Consult your Safety Officer before removing any hoses to vacuum

pumps, gas lines, pressure gauges, or exhaust hoses; they may be contaminated with chemicals used inside the glove box.

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Now that the installation of your glove box is completed, you are ready to use it.

Read this chapter to learn about:

• Routine Daily Work Procedures.

• Hazardous Use with Chemicals.

• Prohibited Acid Use.

• Auto Pressure Controller operation.

Routine Daily Work Procedures

Planning

• Thoroughly understand procedures and equipment required before

beginning work. Work from a written plan.

• Consult your Safety Officer for personal protective equipment

recommendations and operating pressure confirmation.

Start-up

Loading Materials and Drying Train Set-Up

• Basic start-up procedure includes: turning on the vacuum pump,

opening the inert gas valve on the gas supply tank, and turning on the glove box light. The accessory AtmosPure Re-Gen Gas Purifier can be started once an inert atmosphere below 5000 ppm is established.

• Load only the materials and equipment required for the procedure.

Do not overload the glove box. When loading, ensure the inner door is closed before opening the outer door. Then close the outer door.

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Chapter 5: Using Your Glove Box

• Perform evacuation and fill cycle on the transfer chamber using the

vacuum outlet and gas inlet valves. Depending on the vacuum level achieved, it takes 1-5 cycles to reduce oxygen and moisture levels below 1%. Porous materials being transferred may require longer sustained vacuum purges to remove the oxygen and moisture trapped in the materials.

• After transferring materials to the main glove box chamber, perform

the required evacuation and fill cycles (1-499) on the main chamber using the vacuum outlet and gas inlet valves. Normally 75-100 evacuation and fill cycles will reduce moisture and oxygen levels below 1% (see Appendix D). An additional 100 cycles will reduce moisture and oxygen levels below 0.3% or 3,000 ppm. The use of the accessory AtmosPure Re-Gen Gas Purifier can now be turned on.

The use of external glove port covers will expedite the evacuation and fill cycle times and help maintain low moisture and oxygen levels when the glove box is not in use.

• Maintaining a slight main chamber pressure difference of -1" to +1"

water gauge can help to preserve the glove box atmosphere. The moisture/oxygen permeation is reduced at slight pressure differences.

• The AtmosPure Re-Gen Gas Purifier is required to further reduce

and maintain oxygen and moisture levels below 0.3%. The

AtmosPure Re-Gen Gas Purifier can maintain oxygen levels below 1 ppm and moisture levels below 1 ppm.

• Monitoring moisture and oxygen levels inside the glove box is

recommended by using accessory monitors (see Chapter 7).

Work Techniques

• Segregate all clean and contaminated materials in the work area.

• Maintain work at a slightly negative pressure if working with

hazardous compounds.

• Continuously run the AtmosPure Re-Gen Gas Purifier to preserve the

inert atmosphere if so desired.

Transferring Materials to the Outside

• All open chemical weigh vessels or containers must be sealed before

removing materials from the glove box main chamber.

• Upon completion of work, the main glove box chamber must be

evacuated and filled approximately 5-10 times to remove airborne contaminants from the work area. The exact number of cycles should be determined by your Safety Officer.

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Chapter 5: Using Your Glove Box

• Objects in contact with previously contaminated internal atmospheres

must be surface decontaminated or bagged before removal from the glove box through the transfer chamber.

• After placing materials to be removed in the transfer chamber and

locking the internal door, the transfer chamber must be evacuated and filled approximately 1-5 times depending on vacuum level achieved. This ensures the removal of any remaining airborne contaminants before the outer door is opened. Never apply a high vacuum purge on sensitive electronic moisture and oxygen monitors. The exact number of cycles should be determined by your Safety Officer.

Shutdown

• To help preserve the controlled atmosphere, leave the glove box main

chamber under slight pressure differences and continuously run the AtmosPure Re-Gen Gas Purifier. The use of the accessory external glove port covers can assist in preserving the inert atmosphere.

• Keep the outer door closed when not in use.

Hazardous Use with Chemicals

Hazardous chemicals may be used safely only when the glove box vacuum pump and pressure relief bubbler are both properly vented and/or filtered to the outside. Consult your Safety Officer for proper

venting and filtering of the vacuum pump exhaust and the pressure relief valve for your application.

Prohibited Acid Use

On stainless steel models, the glove box interior must not be exposed to mineral acid use. The glove box life span will be limited with use of mineral acids.

NOTE: Labconco makes no claims about corrosion resistance of the

glove box with mineral acid use and warranties will become void.

Auto Pressure Controller Operation

The Auto Pressure Controller performs three automatic modes on the glove box. First, it cycles the evacuating/filling process in the main glove box chamber up to 499 cycles and in the transfer chamber up to 199 cycles. Second, it regulates vacuum/pressure between -5 and +5 inches of water gauge in the main glove box chamber through the glove box pressure control program mode. Third, it regulates vacuum in the transfer chamber between 0 and -29 inches of mercury during the evacuating/filling process.

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Chapter 5: Using Your Glove Box

Front Panel Description

The front panel includes a large display screen and touchpad keys that are adjacent to the LCD display. The operation of these keys will be defined by text on the display screen (LCD). Each key may perform a different operation depending on the control mode selected.

Rear Panel Description

The rear panel includes the power inlet and circuit breakers, power on/off switch, the outlet receptacle for plugging in a vacuum pump (the receptacle varies for 115 volt and 230 volt models), and electrical connection for the glove box pressure control footswitch. The 4 electric solenoid valves that provide control of the glove box Main Chamber gas in/gas out, and Transfer Chamber gas in/gas out are also located on the rear panel. See the separate mechanical installation instructions for plumbing connections to these valves in Chapter 3 and Figure 3-4.

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Chapter 5: Using Your Glove Box

Main Modes of Operation

The Labconco Glove Box Pressure Controller display has four main screens that provide access to the 4 modes of operation listed below.

1.) Glove Box Pressure Control Mode

2.) Glove Box Purge and Fill Mode

3.) Transfer Chamber Purge and Fill Mode

4.) Setup Mode

Press the MODE key at the upper-left hand corner of the display screen to switch between each of these four operation modes. Please be aware that if one of the glove box modes is running/started, other glove box operation modes cannot be used. For example, if the Glove Box Pressure Control mode has started (using the start key), the menu page for the Glove Box Purge/Fill mode cannot be used – this prevents operational control conflicts from occurring at the same time.

Mode Key

Mute/Alarm

Increase

Lower Limit

Decrease

Lower Limit

Manual

Purge

Start/Stop

Setup

Increase Upper Limit

Decrease Upper Limit

Manual

Fill

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Mode Key

Mute/Alarm

Increase

Lower Limit

Decrease

Lower Limit

Chapter 5: Using Your Glove Box

Glove Box Pressure Control – Operation Mode 1

The Pressure Control mode will allow the user to set a high pressure limit up to +5 inches water column and a low pressure limit to -5 inches water column [+/- 12.6 mBar or +/- 1245 Pacals]. Set these high and low pressure limits with the keys adjacent to the “UP” and “DOWN” arrows on the display. Once these limits are set, and the START/STOP key is pressed, the Pressure Controller will automatically control the gas inlet valve, gas outlet valve, and the power to the outlet receptacle for a vacuum pump. Pressure in the Glove Box Main Chamber will be displayed inside the glove box icon on the display screen.

Manual control keys for evacuation “PURGE” and “FILL” (located along the bottom of the display) will allow the corresponding valves to be controlled by the user to increase or decrease the pressure inside the glove box. Note: These manual

keys will not operate the valves when the pressure is outside the limits of +/- 5 inches of water [+/- 12.6 mBar]. A three-position footswitch is included with the

Automatic Pressure Controller. The footswitch provides manual foot control of the gas inlet and vacuum outlet valves. The pressure inside the glove box will be displayed inside the glove box icon on the display screen.

An audio alarm will actuate if the pressure exceeds the maximum limits of +/- 5 inches of water. An “alarm reset” key (identified by the musical icon) is provided to mute this alarm. The alarm will reset automatically when the pressure returns within +/- 5 inches [+/- 12.6 mBar].

Manual

Purge

Manual

Fill

Start/Stop

Setup

Increase Upper Limit

Decrease Upper Limit

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Mode Key

Mute/Alarm

Increase

Lower Limit

Decrease

Lower Limit

Chapter 5: Using Your Glove Box

Glove Box Evacuation (Purge)/Fill – Operation Mode 2

The Glove Box Purge/Fill mode uses the same solenoid valves on the glove box that are used in the Pressure Control Mode. The Pressure Control mode will be temporarily suspended while a “Purge/Fill” command is being executed.

Note that this display screen looks similar to the Pressure Control mode described previously. However, the “SETUP” key has changed to a “CYCLES” key. Up to 499 cycles can be set for automatic “Purge/Fill” of the glove box main chamber by holding the “Cycles” key. To decrease the number cycles, press the “CYCLES” key to 499 cycles and go around again until a lower cycle quantity appears. Use this display screen to also set the low pressure level for purging and the high pressure level for filling by adjusting the upper and lower limit keys. The Labconco Pressure Controller allows the glove box Main Chamber pressure levels to be set to +/- 5 inches of water column [+/- 12.6 mBar]. Once the pressure limits and number of cycles have been selected, press the key adjacent to “START/STOP,” and the automated purge and fill process will begin and continue for the number of cycles selected. Each cycle number will be displayed during the process. To cancel the automated purge and fill process, press the key adjacent to “START/STOP” and the process will be stopped.

Note that when the “Glove Box Purge/Fill” mode is in operation, there are no keys available to allow for system setup as the “CYCLES” key replaced the “SETUP” key.

Manual

Purge

Manual

Fill

Start/Stop

Cycles (up to 499)

Increase Upper Limit

Decrease Upper Limit

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Mode Key

Mute/Alarm

Increase

Lower Limit

Decrease

Lower Limit

Chapter 5: Using Your Glove Box

Use the “MODE” key in the upper left hand corner adjacent to the icon to return to the “Glove Box Pressure Control” screen to use the “Select” mode.

Transfer Chamber Evacuation (Purge) and Fill – Operation Mode 3

The Transfer Chamber Purge/Fill mode controls the pressure changes inside the transfer chamber. Pressure in the transfer chamber will be displayed inside the transfer chamber icon on the display screen. This operation mode controls the gas inlet and gas outlet solenoid valves connected to the transfer chamber.

The user may select up to 199 “Purge/Fill” cycles by adjusting the cycles increase and decrease arrow keys. The user can select the level of pressure inside the transfer chamber during each purge between 0 and -29.5 inches of Mercury/Hg [0 -999 mBars] by adjusting the lower limit keys. Once the lower pressure limit and number of cycles have been selected, press the key adjacent to “Start,” and the automated purge and fill process will begin and continue for the number of cycles selected. Each cycle will be displayed during the process. To cancel the automated purge and fill process, press the key adjacent to “Stop” and the process will be stopped.

Manual

Purge

Manual

Fill

Start/Stop

Setup

Increase Cycles

Decrease Cycles

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Chapter 5: Using Your Glove Box

Setup Menu – Operation Mode 4

Pressing the key opposite the word “Setup,” allows access to the setup operation mode. In the setup mode, the user may select their preferred units of pressure that are displayed, restore settings back to the default state, or calibrate the pressure sensors. The keys adjacent to the up and down arrows on the lower right of the display are used to select one of the three options. After toggling up or down, push the NEXT key to select the option.

Back to

Screen

Option 1

Units of Measure: If this option is selected, the key adjacent to “Next” is used to select pressure units of inches of water, inches of mercury or mBar.

Helpful conversions are as follows:

1 mBar = .39 inches of water column 1 mbar = .0292 inches of mercury 1 inch of water = 2.52 mBar = .0737 inches of mercury 1 inch of mercury = 34.25 mBar

All pressures are measured with respect to atmosphere pressure.

Select Key Up

Select Key Down

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Chapter 5: Using Your Glove Box

Option 2

Restore Defaults: The restore defaults option restores all control settings to the initial programmed settings which are listed in the back of these operating instructions.

Option 3

Calibration: The pressure sensors inside the controller can be calibrated using atmospheric pressure (single point calibration) or atmospheric pressure and a specific pressure level (two point calibration). Two point calibration provides increased accuracy; however, Labconco recommends that only the single point calibration be used because it does not require a calibrated pressure measurement standard. When “Calibration” is selected, the display screen below appears. The keys adjacent to the up and down arrows on the lower right of the display are used to select one of the options. After toggling up or down, push the NEXT key to select the option.

Select Key Up

Select Key Down

Back to

Screen

Note: If option 2 or 3 above is selected, an accurate, traceable pressure measuring device must be connected to the Labconco pressure sensors. Consult Labconco for further instructions for this type of calibration.

Option 1 above is normally selected. Both doors must be open so atmospheric pressure is available to both the glove box main chamber and transfer chamber. Select the NEXT key to advance the screen to sensor offset for Option 1. The display screen changes are shown on the next page for Option 1.

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Chapter 5: Using Your Glove Box

Back to

Screen

Yes, All

Doors

Open

To calibrate the pressure sensors for both chambers, the doors must be open so that atmospheric pressure is present at both sensors. This is the “zero point” for both sensors. Both pressure gauges on the glove box will also read “zero.”

With both doors open, press the key adjacent to “Yes.” Once this is done, the following display screen on the next page appears:

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Main Glove

Box Chamber

Select Key

Chapter 5: Using Your Glove Box

Back to

Screen

Save

Zero

Point

This display screen confirms that the doors are open and the sensors are ready for auto-zeroing. First, select the Main Glove Box Chamber key (Box) and wait for complete calibration to zero pressure. Next, select the Transfer Chamber key (CH) and wait for complete calibration to zero pressure. Once the key adjacent to “Save” is pressed, the new sensor offset valves for both chambers are stored, and this pressure becomes the zero point for both chambers. Select the back key two times

when calibration is complete to

return to the three main modes of operation.

Pressure Control Foot Switch (Labconco #5243600)

Transfer Chamber Select Key

The three position (increase gas/fill, off, purge/decrease gas) foot switch should be plugged into the back panel of the Labconco Pressure Controller. The Foot Switch has the same effect as pressing the Purge and Fill keys on the display panel. The action of the Foot Switch depends on what mode of operation is shown on the display. If the transfer chamber screen is shown on the display, the Foot Switch will control the Transfer Chamber gas in and gas out valves. If the display shows the Glove Box Pressure Control or the Glove Box Purge/Fill, the Foot Switch will control the Glove Box Main Chamber gas in and gas out valves. The foot switch will not work when the pressure/vacuum inside has exceeded the user preset limits shown on the display.

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Chapter 5: Using Your Glove Box

Auto Pressure Controller Specifications

Glove Box

Operating Range Default Settings

Pressure Control

Pressure Control Limits -5 inches of water column to

+5 inches of water column;

+/- 5 inches of water column; [+/- 12.6 mBar].

[+/- 12.6 mBar]

Manual Control Available Purge (Gas out);

Fill (Gas in)

Audio Alarm level Whenever pressure

outside limits

Glove Box Purge/Fill

Purge/Fill Pressure Limit +/- 5 inches of water column;

Manual Control Available Purge (Gas out);

+/- 5 inches of water column;

[+/- 12.6 mBar].

Fill (Gas in)

Number of Purge/Fill

0 to 499 – main chamber 1 Cycles Audio Alarm level Whenever pressure

outside limits

Transfer Chamber Purge/Fill

Purge Pressure Limit -29.5 inches of Mercury/Hg

[-999 mBars] Fill Pressure Limit 0 inches of Mercury/Hg

[0 mBars](atmosphere) Number of Purge/Fill

0 to 199 – transfer chamber 1

-29.5 inches of Mercury/Hg [-999 mBars] 0 inches of Mercury/Hg [0 mBars](atmosphere)

Cycles Audio Alarm level -29.5 inches of Mercury/Hg

[-999 mBars]

Whenever pressure/vacuum outside limits

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CChhaapptteerr 66:: MMaaiinnttaaiinniinngg YYoouurr GGlloovvee BBooxx

Review this chapter on maintenance for the following:

1. Routine maintenance.

2. Initial certification.

3. Recertification.

4. Fluorescent light replacement.

5. Solenoid valve replacement for Auto Pressure Controller, if equipped.

6. Auto Pressure Controller circuit board replacement.

Routine Maintenance Schedule

Weekly

• Wipe down the interior surfaces of the glove box with a disinfectant

or cleaner, depending upon the application.

• Using an appropriate cleaner and cloth, clean the exterior surfaces of

the glove box, particularly the front and top, to remove any accumulated dust.

• Chart the pressure decay of the glove box, noting the change in

pressure over short time periods. Adjust as necessary for temperature and barometric pressure changes. See Chapter 3: Validating the Glove Box.

• If required, monitor and record oxygen and moisture levels inside the

glove box with accessory monitors (see Chapter 7).

• Check inlet inert gas pressure to ensure it never exceeds 10-14 psi

(69-96 kPa).

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Chapter 6: Maintaining Your Glove Box

Annually

• Replace the fluorescent lamps.

• Have the glove box certified by a qualified technician. See

Certification and Recertification in Chapter 6.

Initial Certification

Each Protector Glove Box has been leak tested at the factory with helium gas at positive 5" w.g. pressure; no leaks greater than 1 x 10-6 cc/sec are allowable. The glove box should be certified by your Safety Officer before use, with one of the two tests listed below under Recertification. See Appendix G for Labconco Helium Leak Test Method performed on all glove boxes at the factory.

Recertification

Under normal operating conditions, the glove box should be recertified at the time of installation and at least annually thereafter. The certifier should choose and perform one of the following tests. Refer to Validating the Glove Box in Chapter 3.

• Oxygen Method Leak Test per ISO 10648-2 test methods.

• Pressure Change Leak Test per ISO 10648-2 test methods.

Fluorescent Light Replacement

1. Disconnect the power. Refer to Figure 6-1 for replacement.

2. Locate the upper front panel.

3. Remove the front panel by removing two screws located at the bottom

of the front panel on both sides.

4. Remove the old fluorescent lamps.

5. Reinstall the new fluorescent lamps and front panel by reversing the

steps above.

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Chapter 6: Maintaining Your Glove Box

Front panel screw location

Fluorecent

Bulbs

Figure 6-1

Fluorescent Lights

Fluorescent Bulb Spring Clip

Fluorescent Bulb Light Socket

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Chapter 6: Maintaining Your Glove Box

Solenoid Valve Replacement

1. Decontaminate the glove box interior, if required.

2. Wear appropriate protective equipment as determined by your Safety

Officer.

3. Turn the glove box and vacuum pump OFF. Turn the manual valves

on the control panel to closed.

4. Remove the rear access panel and the solenoid valve located on the

rear of the Auto Pressure Controller. See Figures 3-4 and 4-1.

5. Replace with the new solenoid valve with proper IN or OUT airflow

direction. Vacuum is OUT and Inert Gas is IN.

6. Turn the glove box ON and re-certify.

Auto Pressure Controller Circuit Board Replacement

1. Disconnect the power. Refer to Figure 6-2. Check with your Safety

Officer for contaminated hoses and seal the ends with tape.

2. Remove the control panel supported by four screws.

3. Remove the circuit board from the control panel and replace with the

new circuit board.

4. Replace the pressure hoses as shown in Figure 6-2.

5. Reinstall the control panel with the new circuit board.

6. Calibrate the pressures of both chambers per the Auto Pressure

Controller Calibration procedure in Chapter 5.

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Chapter 6: Maintaining Your Glove Box

Typical circuit board mounting screw

Transfer chamber pressure hose connection attached to port nearest circuit board.

Typical circuit board connectors

Main chamber pressure hose connection attached to port nearest circuit board.

Figure 6-2

Auto Pressure Controller Circuit Board

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CChhaapptteerr 77:: AAcccceessssoorriizziinngg YYoouurr GGlloovvee BBooxx

There are many ways to accessorize and modify the controlled atmosphere glove box for your individual requirements. These include many different accessories listed in this chapter.

1. Adjustable Height Base Stands with Work Surface (Part

#5235500 w/casters, Part #5235501 w/adjustable feet, Part #5062020 Double Mobile Base Stand)

These single stands, 60" x 31", with attached black laminate work surfaces have an adjustable height range from 33" to 40" (838mm1,016mm) and include a lower shelf for supporting accessory equipment such as the vacuum pump and AtmosPure Re-Gen Gas Purifier. See Appendix B for dimensions of Double Mobile Base Stand for doublewide glove boxes.

2. Anti-Static Ionizer Fan (Part #5234400, 100/115V or

Part #5234401, 230V International)

Used for weighing operations. The ionizer fan circulates ionized air inside the glove box to reduce static charge. Ionizer Replacement Points (set of 8 emitter electrodes) are ordered separately (Part #5235100). See Appendix F for additional information.

3. Balance Vibration Isolator (Part #5234600)

Isolates building vibrations and aids with balance weighing. Includes a 20" x 13" x 2 1/4" marble slab with four isolator pads and type 304 stainless steel top cover to protect the porous marble.

4. Vacuum Aspirator Kit

Includes the valve, hose, canister, and vacuum pump for evacuating fluids around the work surface.

Part # Descriptions

5241800 Vacuum Aspirator Kit, 115V 5241801 Vacuum Aspirator Kit, 230V International

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Chapter 7: Accessorizing Your Glove Box

5. Electrical Power Strips

These convenience electrical power strips are available in US and international versions and plug into the interior receptacle of the glove box.

Part # Descriptions

5211500 US Power Strip 115V, 4 Position, 10A 5210601 UK Power Strip 250V, 6 Position, 10A 5290401 European Power Strip 250V, 4 Position, 10A 5332601 Australian Power Strip 250V, 6 Position, 10A

6. Electrical 9 Pin Sealed Pass-Through Kit (Part #5075619)

Includes sealed 9 pin (D-subminiature with male pins on one side and female pins on the other) connector and cord for data transfer outside the glove box. Typical data transfer includes oxygen/moisture levels or analytical balance measurements to a printer.

7. Gas Inlet / Vacuum Outlet Tubing Kits and Direct Drive Vacuum

Pumps

These flexible vinyl tubing kits provide the necessary tubing and fittings to make connections to an inert gas supply tank and a rotary vane vacuum pump. See Figures 3-4 and 3-7.

Part # Descriptions Used With

5245100 Complete Gas/Vac Tubing Kit (Required) Manual Valves, all Protector Glove Boxes 5245200 Complete Gas/Vac Tubing Kit (Accessory) Auto Pressure Controller, accessory only 1472100 Direct Drive Vacuum Pump, 115V, 50/60Hz All Glove Boxes 7739400 Direct Drive Vacuum Pump, 230V, 50/60Hz,

NEMA 6-15P plug

7739402 Direct Drive Vacuum Pump, 230V, 50/60Hz

with reverse IEC plug

All Glove Boxes, International Version

8. AtmosPure Re-Gen Gas Purifiers (see catalog #’s below)

The AtmosPure Re-Gen Purifier produces an ultrapure inert atmosphere for materials sensitive to moisture and/or oxygen by circulating an inert gas and removing oxygen and moisture inside the box. Compatible with any Protector or Precise Controlled Atmosphere Glove Box, the AtmosPure allows the boxes to meet or exceed ISO 10648-2 test methods. The Purifier has the capacity to remove up to five liters of oxygen (13,500 ppm) and 660 grams of moisture (18 million ppm) at standard temperature and pressure (STP). Depending on the application, glove box liner material and frequency of use, regeneration may be necessary only once every one to three months and completed in only 13 hours. See Figure 1-1 in Chapter 1.

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Chapter 7: Accessorizing Your Glove Box

Part # Descriptions Electrical Receptacle & Plug Type

5121810 100-115 volts, 50/60 Hz North America, 100-115 Volts 5121831 208-230 volts, 50/60 Hz British (UK) 5121835 208-230 volts, 50/60 Hz Schuko 5121840 208-230 volts, 50/60 Hz China/Australia 5121845 208-230 volts, 60 Hz North America, 208-230 Volts

9. AtmosPure Regeneration Plumbing Kit (Part #5441700)

The regeneration plumbing kit includes flexible tubing and fittings to make all the regeneration service supply tubing connections. These are the connections to the 4% hydrogen inert gas mixture, vacuum, and vent.

10. AtmosPure Circulation Kits (see Part #’s below)

The circulation kits include all the plumbing and tubing parts needed to connect the Gas Purifier to the glove box. There are two options for neoprene lined hose or stainless steel tubing.

Part # Description Used On

5441800 3/4" I.D. Neoprene lined hose All Glove Boxes, single width 5441801 3/4" I.D. Neoprene lined hose All Glove Boxes, double width 5441802 3/4" Rigid Stainless Steel Protector Glove Boxes, single width 5441803 3/4" Rigid Stainless Steel Protector Glove Boxes, double width

11. AtmosPure Roughing Pre-Filter Kit (Part #5441500)

Provides two roughing particulate pre-filters and clamp plates to mount inside Protector Glove Boxes only. It is used to filter the inlet and outlet of the glove box as the inert gas is purified. Order individual 5122400 filters separate. Two each required.

12. AtmosPure P100 Particulate Filter Kit (Part #5441600)

Provides two P100 particulate filters and mount housings that are similar to respirator cartridges. P100 filters are 99.97% efficient for all particulates. It is used to filter the inlet and outlet of the glove box as the inert gas is purified. Order replacement filter set 5441601 separate which includes two P100 particulate filters.

13. Oxygen Monitors

Oxygen monitors allow the user to continuously monitor oxygen levels inside the glove box. Monitors are normally placed inside the main chamber to provide the best performance. Never operate a

transfer chamber vacuum evacuation on sensitive oxygen monitors as damage can occur.

Part # Descriptions

5244500 0-10,000 ppm Oxygen Monitor (with three ranges of 0-100 ppm, 100-

1,000 ppm, and 1,000-10,000 ppm) 100V/115V – 50/60 Hz

5244501 0-10,000 ppm Oxygen Monitor (with three ranges of 0-100 ppm, 100-

1,000 ppm, and 1,000-10,000 ppm) 230V – 50/60 Hz – International

5244400 0.1-100% Oxygen Monitor (1,000 ppm lowest) 100V/115V/230V –

50/60 Hz

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Chapter 7: Accessorizing Your Glove Box

14. Moisture Monitors/Hygrometers

Moisture monitors or hygrometers allow the user to continuously monitor moisture levels inside the glove box. Moisture monitors are normally placed inside the main chamber to provide the best performance. Never

operate a transfer chamber vacuum evacuation on these sensitive hygrometers as damage can occur.

Part # Descriptions

5244900 1-1000 ppm Moisture Monitor/Hygrometer (Factory Set at 1-

1,000 ppm, with three settings of 1-10 ppm, 1-100 ppm, and 11,000 ppm) 100V/115V – 50/60 Hz

5244901 1-1000 ppm Moisture Monitor/Hygrometer (Factory Set at 1-

1,000 ppm, with three settings of 1-10 ppm, 1-100 ppm, and 11,000 ppm) 230V – 50/60 Hz – International

5244800 0-100% Hand Held Digital Hygrometer with Temperature

Display; measures relative humidity and temperature

5442200 Moisture Meter Probe and Calibration Stand -80 to 0°C dew

point range or 0 to 6000 ppm (Display is in dew point only), 100-115V, 50-60 Hz

5442201 Moisture Meter Probe and Calibration Stand -80 to 0°C dew

point range or 0 to 6000 ppm (Display is in dew point only), 230V, 50-60 Hz

15. Exterior Monitor Plumbing Kits (Part #’s below)

The exterior monitor plumbing kits provide attachment plumbing to oxygen or moisture monitors outside the glove box. Note: The control and accuracy of the oxygen and moisture monitors will be reduced due to the surrounding ambient air rather than inert gas inside the glove box.

Part # Descriptions Used With

5442400 Plumbing Kit for Oxygen Monitor (0 to 10,000 ppm) 5244500, 01 5442500 Plumbing Kit for Monitor/Hygrometer with pump (0 to 10,000 ppm) 5244900, 01

16. Automatic Pressure Controller (for use with manual control

Protector Glove Boxes only)

The Pressure Controller provides the operator the convenience of automatically regulating pressure and vacuum within the glove box main chamber and transfer chamber. The end user can also automate the evacuation/fill cycles up to 499 cycles for the main chamber and up to 199 cycles on the transfer chamber. The vacuum and pressure can be automatically regulated between -5 to +5 inches of water gauge in the main chamber and between 0 to -29 inches of mercury gauge in the transfer chamber. See illustration below and figures in Chapter 3.

Part # Electrical Configuration

5238600 100/115 Volts, 50/60 Hz operation 5238601 230 Volts, 50/60 Hz operation 5239100 Benchtop Bracket (required and ordered separate)

Product Service 1-800-522-7658

Chapter 7: Accessorizing Your Glove Box

17. AtmosPure 6-pin Communication Cable (Part #5442000)

Required for use when AtmosPure and glove box configured with Auto Pressure Controller are sharing one vacuum pump.

18. AtmosPure 9-pin Oxygen/Moisture Monitor Cable (Part #5442100)

Required for use when the AtmosPure is used in AUTO mode. Allows for automatic control of the AtmosPure blower from an accessory Oxygen or Moisture Monitor (sold separately).

19. AtmosPure 3/4" to 3/8" Adapter Kit (See Part #’s below)

Fittings permit 3/4" ID neoprene hose included with 5441800 and 5441801 Kits (sold separately) to attach to separate filtration components listed under item 20 below. Two fittings included with each kit.

Part # Description

5441900 Elbows 5441901 Reducers

20. Filtration Components for Moisture, Oxygen and Fume Removal

The following components can be used to remove organics, acids, ammonia, oxygen, moisture and other gases as indicated. They are connected to the two auxiliary valves on the main glove box chamber for recirculation of main chamber atmosphere and contaminant removal of air.

Part # Descriptions

5248200 Vacuum Pump for Circulation, 115V, 60 Hz w/switch 5248201 Vacuum Pump for Circulation, 230V, 50 Hz International 7815301 Canister with Stand for Traps 7815200 Moisture & Solvent Trap Insert; to provide less than 50 ppm

moisture (molecular sieve) 7814800 Acid Vapor Trap Insert 7815000 Radioisotope Trap Insert 7995600 Ammonia Trap Insert 5244100 Flowmeter, Visual 5244200 Oxygen and Moisture Removal Column catalysts can be

replaced but not regenerated; to provide less than 5 ppm

oxygen and less than 50 ppm moisture (refillable) 5244300 Oxygen and Moisture Removal Column Support Stand

Complete Moisture Drying Train; includes pump, column(s), stainless steel tubing and molecular sieve to achieve less than 5ppm moisture only.

Part # Descriptions

5061300 115V, 60 Hz – one column 5061301 115V, 60 Hz – two columns, one in use while other regenerates 5061302 230V, 50 Hz – one column 5061303 230V, 50 Hz – two columns, one in use while other regenerates 5117000 Optional Second Column for 5061300 or 5061302 7837300 Molecular Sieve Replacement Media for two refills

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Chapter 7: Accessorizing Your Glove Box

21. Drying Train Tubing Kit

Includes the (FEP) tubing and connections required for connecting typical drying train components such as moisture, oxygen, and other gases. Fluorinated ethylene-propylene (FEP) tubing has very low moisture absorption.

Part # Descriptions

5242500 Drying Train Tubing Kit; moisture, solvents and radioisotopes 5242501 Drying Train Tubing Kit; oxygen and moisture only

22. Gas and Vacuum 0.2 Micron Filter (Part #5240100)

Used for removal of particulates in a drying train, vacuum pump exhaust or inert gas line. 0.25"-0.38" ID tube connection with additional fittings for attachment to 0.50" ID hose.

23. External Glove Port Covers (Part #5242200)

Cap and clamp attaches to the glove ports to eliminate glove inflation during purging and filling operations and reduce the cycle time of the purging/filling process. Sold as a set of two.

24. Interior Glove Port Cover (Part #5060400)

Quickly seals glove box should sudden leak occur due to glove damage. Stainless steel with molded rubber gasket and spring-loaded latches with adjustable closure knob.

25. Interior Shelves (Part #5061600)

Provide shelf space across back wall of glove box interior. Three stainless steel storage shelves are attached to epoxy-coated steel upright supports. Shelves have adjustable height and measure 30" wide x 6" deep. Installation hardware and instructions included.

26. Gloves (see Appendix A: Replacement Part numbers for other

glove sizes and material types)

Neoprene gloves are the most resistant to abrasion and tearing. Butyl gloves provide higher impermeability and improved dexterity. Hypalon gloves are made with a mixture of neoprene and butyl rubber to provide an intermediate level of neoprene and butyl features.

27. Left Side Sealed Electrical Plug Kit (see Part #’s below)

Part # Descriptions

5075622 115V single outlet 5075623 230V IEC single outlet

28. Vacuum Line HEPA Filter and Housing

Part # Descriptions

5441400 Housing for HEPA Filter and connections 5441401 HEPA Filter, Round 99.99% efficient on 0.3 micron 5441403 Pressure Relief Bubbler Room Exhaust Kit for HEPA filtration

Product Service 1-800-522-7658

Chapter 7: Accessorizing Your Glove Box

29. Left Side Door Option (Factory Prepared Only)

Any stainless steel glove box can be factory prepared with a large sealed left side door for use in conveniently loading large equipment. With the left side door option, the need to remove the front laminated safety glass frame is eliminated. The left side door has an opening of

16.5" (419 mm) wide x 23.2" (589 mm) high. Contact Labconco at time of order for the left side door option.

Figure 7-1

Left Side Door Option

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Refer to the following table if your glove box fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance.

PROBLEM CAUSE CORRECTIVE ACTION

Lights will not operate.

No power to electrical receptacle.

Auto Pressure Controller Solenoid Valves will not operate.

Auto Pressure Controller will not operate.

Unit not plugged into outlet. Plug unit into appropriate electrical service.

Circuit breaker(s) tripped. Reset or replace circuit breaker.

Lamp not installed properly. Inspect lamp installation.

Lamp wiring disconnected. Inspect lamp wiring.

Defective lamp. Replace lamp.

Light switch is defective. Replace light switch.

Defective electronic ballast. Replace ballast. Unit not plugged in. Plug unit into electrical service.

Circuit breaker(s) tripped. Reset or replace circuit breaker.

Receptacle switch is defective.

Receptacle is defective. Replace receptacle. Plumbing is installed incorrectly.

Solenoid Valve is defective. Replace solenoid valve. Unit not plugged into outlet. Circuit breaker(s) tripped.

LCD Display is defective. Replace circuit board or power supply

Replace receptacle switch.

Check plumbing and insure VAC is “out” to vacuum pump and GAS is “in” from inert gas supply.

Plug unit into appropriate electrical service. Reset or replace circuit breaker.

circuit board.

Product Service 1-800-522-7658

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The components that are available for your glove box are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service.

Item Qty. Part Number Description

1A 1 5066600 Window, Glass Viewing 1B 1 5066700 Window, Polycarbonate Viewing 2A 1 5067200 Window, Gasket, Fiberglass Models 2B 1 5098400 Window, Gasket, Stainless Steel Models 2C 0.01 quart 1576800 Adhesive, Plyobond, Fiberglass Models 2D 0.01 quart 1594800 Adhesive, 3M #4799, Stainless Steel Models 3A 1 pair 1640600 9 ¾ size Neoprene Glove (standard) 3B 1 pair 1640601 9 ¾ size Butyl Gloves 3C 1 pair 1640602 9 ¾ size Hypalon Gloves 3D 1 pair 1640500 8 ½ size Neoprene Gloves 3E 1 pair 1640501 8 ½ size Butyl Gloves

3F 1 pair 1640502 8 ½ size Hypalon Gloves 3G 2 each 1640000 O-rings 3H 2 each 1965600 Clamp – O-Ring Gloves

4A 1 5410800 Complete Door Assy., Fiberglass Inner 4B 1 5098500 Complete Door Assy., Stainless Steel Inner 5A 1 5422700 Inner Acrylic Door Only, Fiberglass Models Only 5B 1 5064200 Door Gasket, Inner and Outer, Fiberglass Models Only 5C 0.1 tube 1594800 Gasket Sealant, Fiberglass Only

6 1 5410000 Outer Acrylic Door Only, Fiberglass Models Only 7A 1 5098300 Door Gasket, Inner and Outer, Stainless Models Only 7B 0.1 tube 1594800 Gasket Sealant, Stainless Steel Models Only, 3M #4799

8 1 5414000 Complete Door Assy., Fiberglass Outer

9 1 5098600 Complete Door Assy., Stainless Steel Outer

10A 1 5068601 Clamp, Door Plated Steel, Fiberglass Models 10B 1 5068600 Clamp, Door Stainless Steel Models

11 1 5064600 Door Adjustment Screw 12 1 5410000 Outer Acrylic Door Only

13 1 5411500 Ball Valve, Control Panel 14A 1 5417600 Solenoid Valve, Pressure Controller Only, 115V 14B 1 5417601 Solenoid Valve, Pressure Controller Only, 230V 15A 1 1408200 Nut, Compression 15B 1 1408300 Ferrule (Large) 15C 1 1408400 Ferrule (Small)

16 1 liter bottle 1988000 Oil, Bubbler

17 1 1410100 Union, Straight 3/8 T

18 1 5427600 Bubbler with Liquid Level Gauge

19 1 5425100 Elbow, 3/4

20 1 5425200 Plug, 3/4

21 1 1365200 Pressure Gauge, ±5" H2O, Basic Manual Units Only

22 1 1365300 Vacuum Gauge, 0-30” Hg, Basic Manual Units Only 23A 1 1409900 Insert, Tube (used inside tubing to gauges) 23B 2 5426600 Tubing, 3/8 O.D. x 3/16 I.D. cut to length 23C 2 6964400 Fitting, Barbed Tube 23D 4 ft. 1554304 Tubing, Tygon 5/32 O.D. x 3/32 I.D.

Window Components

Transfer Chamber Components

Plumbing Components

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Appendix A: Replacement Parts

Item Qty. Part Number Description

24A 1 1307000 Switch, 2 Position

24B 1 3823600 Retainer, Switch

25 1 5240000 Membrane Switch Decal, Auto Pressure Controller Only 26 1 5053400 Control Board, Auto Pressure Controller Only 27 1 5053700 Power Supply Board 12VDC/5.2A, auto Pressure Controller Only 28 1 5243600 Foot Switch, Auto Pressure Controller Only

29A 1 1327211 Circuit Breaker, 12A

29B 1 1327203 Circuit Breaker, 6A 29C 1 1327201 Circuit Breaker, 10A

29D 1 1327200 Circuit Breaker, 3A

29E 1 1327205 Circuit Breaker, 2A

30 1 1333800 Inlet Power, IEC Connector

31A 1 7907802 Outlet Assy., VAC Pump 115V

31B 1 1333900 Receptacle Outlet IEC, VAC Pump 230V

32A 1 1230700 Duplex Receptacle, Interior 100-115V, N. America

32B 1 1295200 Receptacle, UK 230V 32C 1 1295300 Receptacle, Schuko 230V

32D 1 1295100 Receptacle, China/Australia 230V

32E 1 1283900 Duplex Receptacle, Interior 230V, N. America

32F 1 5233400 Gray Duplex Wallplate, 100-115V or 23V, N. America 32G 1 1294900 Wallplate, UK or Schuko 32H 1 1295000 Cover, China/Australia 33A 1 1305800 Power Cord 100-115V, N. America

33B 1 1332600 Power Cord 230V, UK

33C 1 1336100 Power Cord 230V, Schuko 33D 1 1332700 Power Cord 230V, China/Australia

33E 1 1338000 Power Cord 230V, N. America

34 2 9721901 Lamp, Fluorescent F25T8-SP41, 25 watt 35 2 3836800 Lampholder, Push On 36 1 3838100 Ballast Assembly, Fluorescent

37A 1 5423000 Wiring Diagram, G.B. 115V

37B 1 5423100 Wiring Diagram, G.B. 230V 38A 1 5425400 Wiring Diagram, Auto Pressure 115V

38B 1 5425401 Wiring Diagram, Auto Pressure 230V 39A 1 5437400 Wiring Diagram, Doublewide 115V

39B 1 5437401 Wiring Diagram, Doublewide 230V

40A 1 5413900 Panel Access, Left

40B 8 1909217 Acorn Nut, #10-24

41 1 1934601 Heyco Bushing, Pass-Through

42A 1 5413601 Front Panel, Painted Fiberglass Models

42B 1 5413600 Front Panel, Stainless Steel, Stainless Steel Models

42C 2 1889308 Screw, #10-24 x 0.50 Phillips 43A 1 5252700 Combination Isolation Valve, Interior

43B .01 1584100 Spray, Dry Silicone Lubricant

44 1 5427700 Union, Straight 3/4T (used to exhaust bubbler)

Electrical Components

Miscellaneous

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Appendix A: Replacement Parts

Figure A-1

Replacement Parts

Product Service 1-800-522-7658

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See the following for all dimensions of the glove boxes. For conversion, 1" equals 25.4mm. All dimensions in inches.

Product Service 1-800-522-7658

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This Appendix contains technical information about all the glove boxes including electrical specifications and environmental operating conditions.

• All models: 12 Amps, 100-115V or 230V, 50/60 Hz

• Vacuum pump exterior receptacle rated at 12 Amps, 100-115V, 50/60

Hz or 6 Amps, 230V 50/60 Hz.

• Single width units require two line cords and double width units require

three line cords.

Environmental Conditions

• Indoor use only.

• Maximum altitude: 6562 feet (2000 meters).

• Ambient temperature range: 41° to 120°F (5° to 49°C).

• Maximum relative humidity: 80% for temperatures up to 88°F (31°C),

decreasing linearly to 50% relative humidity at 104°F (40°C).

• Main supply voltage fluctuations not to exceed ±10% of the nominal

voltage.

• Transient over-voltages according to Installation Categories II (Over-

voltage Categories per IEC 1010). Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed.

• Used in an environment of Pollution degrees 2 (i.e., where normally

only non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.

Product Service 1-800-522-7658

1000000

100000

10000

Oxygen (PPM)

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Protector CA (Stainless Steel) Oxygen Removal with AtmosPure Re-Gen Gas Purifier

210000

42000

10000

3300

1000

100

0 50 100 150 200 250

653.6, 220 cycles stop and

AtmosPure circulation blower

starts and isolation valves are

opened.

55.7

26.2

14.1

7.7

4.2

2.4

1.5

1.1

0.8

0.7

0.1

Time (Minutes)

This chart shows oxygen level decrease from 210,000 ppm at atmospheric conditions to 0.7 ppm after 210 minutes. Two separate calibrated oxygen monitors confirmed results.

Product Service 1-800-522-7658

100000

10000

Moisture (PPM)

1000

100

Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates

Protector CA (Stainless Steel) Moisture Removal with AtmosPure Re-Gen Gas Purifier

11253, 40% RH

1560

696

445

87.5

167.5, 220 cycles stop and AtmosPure circulation blower starts and isolation valves are

opened.

15.9

1 10 100 1000 10000

Time (Minutes)

8.5

3.5

2.4

1.8

This chart shows moisture level decrease from 11,253 ppm (40% relative humidity) to 1.0 ppm after 8965 minutes. Two separate calibrated moisture monitors confirmed results.

1.0

Product Service 1-800-522-7658

1000

100

Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates

Protector CA (Stainless Steel) Oxygen Removal with AtmosPure Re-Gen Gas Purifier

753.6

55.7

Oxygen (PPM)

0 20 40 60 80 100 120 140 160

0.1

26.2

14.1

7.7

4.2

2.4

1.1

1.5

0.8

Time (Minutes)

This chart shows oxygen level decrease from 753.6 ppm to 0.7 ppm after 150 minutes. Two separate calibrated oxygen monitors confirmed results.

0.7

Product Service 1-800-522-7658

1000

Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates

Protector CA (Stainless Steel) Moisture Removal with AtmosPure Re-Gen Gas Purifier

853.2

100

Moisture (PPM)

1 10 100 1000 10000

87.5

15.9

8.5

3.5

2.4

Time (Minutes)

This chart shows moisture level decrease from 853.2 ppm to 1.0 ppm after 8905 minutes. Two separate calibrated moisture monitors confirmed results.

1.8

1.0

Product Service 1-800-522-7658

Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates

Protector CA (Fiberglass) Oxygen Removal with AtmosPure Re-Gen Gas Purifier

1000

100

Oxygen (PPM)

969.2

63.9

28.0

0 20 40 60 80 100 120 140 160

0.1

15.3

7.9

4.3

2.4

1.6

Time (Minutes)

1.2

0.9

1.0

This chart shows oxygen level decrease from 969.2 ppm to 0.77 ppm after 150 minutes. Two separate calibrated oxygen monitors confirmed results.

0.77

Product Service 1-800-522-7658

1000

100

Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates

Protector CA (Fiberglass) Moisture Removal with AtmosPure Re-Gen Gas Purifier

600

50.1

Moisture (PPM)

10.6

6.7

4.6

1 10 100 1000 10000

Time (Minutes)

This chart shows moisture level decrease from 600 ppm to 2.3 ppm after 9875 minutes. Two separate calibrated moisture monitors confirmed results.

3.5

2.3

Product Service 1-800-522-7658

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Protector (FRP) Fiberglass

Attainable Oxygen Purity (ppm) 0.4 ppm 0.2 ppm

Usable Oxygen Permeation 0.16 ppm/min 0.14 ppm/min

Approximate Life (ppm) 13,500 ppm 13,500 ppm

Approximate Life (days) 58 67

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Protector Stainless Steel

Product Service 1-800-522-7658

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Static Electricity Test

The tests were performed by monitoring the level of static electricity found on the work surface and interior surfaces within the glove box. Static electricity levels were measured with an AlphaLab Surface DC Voltmeter

http://scientificmeter.com/surface_dc.htm.

A DESCO Emit Mini Zero Volt Ionizer http://www.descoemit.com/ViewProduct.aspx?pid=

50661&h=1323

was installed in the glove box. The DESCO ionizer is a dual steady state DC auto balancing bench top ionizer. The ionizer helps to neutralize static electricity on surrounding surfaces. The time required by the ionizer to neutralize static electricity will be the shortest when objects are within 12" to 36" directly in front of the ionizer. The time for neutralization increases as the distance from the ionizer increases. The time required for sufficient electrostatic decay will also vary depending upon user application, moisture levels within the supply air and the level of cleanliness within the glove box interior.

Product Service 1-800-522-7658

(

Electrostatic with Glove Box Blower & Ionizer Fan OFF 100 CFM Blower & Ionizer Fan ON After 1 Hour 100 CFM Blower & Ionizer Fan ON After 2 Hours

The mini zero volt ionizer’s neutralization time will be shortest when objects are approximately 12" to 36" directly in front of the ionizer and will increase as the distance from the ionizer increases. Set the fan speed switch on the side of the ionizer to the LOW or HIGH position. Higher airflow will result in faster neutralization rates. Position the ionizer so that the maximum airflow is directed at the items or area to be neutralized. The LED will turn on during power up and remain GREEN during normal operation. The ionizer has a grounding jack and must have a good earth ground to maintain proper balance. Always plug the AC power adaptor into the ionizer and then into the appropriate AC power source. The ionizer has no On/Off switch so it should be running as soon as it is plugged in. The ionizer is designed to run off of 24VDC ±10% 300mA.

The balance adjustment for the ionizer can be accomplished by inserting a small screwdriver or trimmer adjustment tool into the balance adjustment hole located on the side of the unit. To increase the output in a positive direction, turn the potentiometer in a clockwise direction. Conversely, to increase the output in a negative direction, turn the potentiometer in a counter clockwise direction. It is recommended that you use a surface DC voltmeter when adjusting the balance of the ionizer.

Under normal conditions, the ionizer will attract dirt and dust (especially on the emitter electrodes). To maintain optimum neutralization efficiency and operation, cleaning with isopropyl alcohol should be performed on a regular basis. If the performance of the ionizer degrades because of dirty or corroded points, the LED on the front of the unit will turn YELLOW; the audible alarm will sound continuously. Under this condition, the unit is not able to maintain balanced ionization. The input power cord must be disconnected before the unit is opened for maintenance. The emitter electrodes should be cleaned using the alcohol cleaners included or a swab wet with isopropyl alcohol. Unscrew the 4 screws on the back of the unit and then remove the screen. After cleaning the emitter electrodes, reinstall the screen and 4 screws. The emitter electrodes should not require replacement during the life of the unit with normal handling. Verify the balance of the ionizer with a surface DC voltmeter after cleaning.

Appendix F: Ionizer Fan Performance Criteria

Test Point Locations and Voltage Levels

Metal

Housing

Inlet

HEPA

+ 420 + 220 + 734 + 375 + 106 + 151 + 153

+ 96 + 114 + 290 + 93 + 45 + 52 + 35

+ 28 + 58 + 51 - 52 - 15 - 20 - 22

Metal Housing Exhaust

HEPA

Metal

Transfer

Chamber

Handle

Back Interior Surface

Left

FRP Interior Surface

Right

FRP Interior Surface

Static Electricity Test Results (VOLTS) on the Glove Box

Neutralization is best between +/-100V)

Center

FRP

Work

Surface

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Purpose

The purpose of this test is to assure that all gasketed joints and seams of the enclosure are helium leak tight.

Test Setup

1. Connect one of the valves to a vacuum pump.

2. Connect the other valve to a helium supply tank regulated between 10

and 15 PSI.

3. Install two neoprene gloves onto the glove ports using standard O-

rings and clamps.

Test Procedure

1. Be sure the inner transfer chamber door is latched closed and the

outer door is open.

2. Open the helium inlet test valve and allow box to pressurize to +5

H2O gauge than close valve. Then with the vacuum pump running, open the test outlet valve and allow the box to be evacuated to -5" H2O gauge then close valve. Repeat this purge/fill for a total of three cycles then close both valves.

3. While Glove Box pressure is held at +5.0" minimum, sniff all Glove

Box joints, seals and seams of the Glove Box shell using a test probe connected to a helium mass spectrometer (Inficon model or equal calibrated at 1.7 x 10-7 STD. CC/SEC). Also sniff the inner transfer door seal by inserting test probe through the outer transfer chamber door. Any detectable leaks greater than 1 x 10 cause for rejection.

4. Close and latch the outer door and open the inner door. Adjust Glove

Box pressure to +5.0".

5. Sniff the outer transfer door seal and make sure there are no leaks

greater than rate specified in Step 3 above.

-6

STD.CC/SEC is

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Appendix G: Helium Leak Test Procedure

Other Equipment

1. For a less critical helium leak measurement using a lower cost leak

detector, Labconco recommends using Restek (www.restek.com) or other instrumentation available from a laboratory supply dealer.

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AAppppeennddiixx HH:: CCoonnvveerrssiioonnss

Pressure Conversions

mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar

From

mmHg

in.Hg

in.H2O

ft.H2O

atm

lb/in.2

Kg/cm2

kPa

bar

1 .03937 .5353 .04461 .00132 .01934 .00136 .1333 .0013

25.40 1 13.60 1.133 .03342 .4912 .03453 3.387 .0339

1.868 .07355 1 .08333 .00246 .03612 .00254 .2490 .0025

22.42 .8826 12 1 .02950 .4334 .03048 2.988 .0299

760 29.92 406.8 33.90 1 14.70 1.033 101.3 1.013

51.71 2.036 27.69 2.307 .06805 1 .07031 6.895 .0689

735.6 28.96 393.7 32.81 .9678 14.22 1 98.05 .981

7.500 .2953 4.016 .3347 .00987 .1451 .0102 1 .01

750 29.53 401.6 33.47 .987 14.51 1.02 100 1

Flow Rate Conversions

From

lit/sec

gal/min

ft3/sec

ft3/min

bbl/hr

bbl/day

lit/sec gal/min ft3/sec ft3/min bbl/hr bbl/day

1 15.85 0.03532 2.119 22.66 543.8

0.06309 1 0.00223 0.1337 1.429 34.30

28.32 448.8 1 60 641.1 1.54x10

0.4719 7.481 0.01667 1 10.69 256.5

0.04415 0.6997 0.00156 0.09359 1 24

0.00184 0.02917 6.50x10

0.00390 0.04167 1

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AAppppeennddiixx II:: RReeffeerreenncceess

Many excellent reference texts and booklets are currently available. The following is a brief listing:

Pharmaceutical Isolators, A Guide to their application design and control. Pharmaceutical Press 2004, Editors: Midcalf, Phillips, Neiger, and Coles.

Isolation Technology, A Practical Guide, 2004 CRC Press, 2nd Edition, www.crcpress.com

International Standard ISO 10648-2 Containment Enclosures

• Classification according to leak tightness and associated checking

methods.

NIOSH Pocket Guide to Chemical Hazards

U.S. Dept of Health and Human Resources Centers for Disease Control and Prevention National Institute for Occupational Safety and Health

The Manipulation of Air Sensitive Compounds, by D.R. Shriver and M.A. Drezdzon; published by John Wiley and Sons.

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Labconco Protector Controlled Atmosphere Glove Box User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual