Labconco Precise HEPA-Filtered Glove Boxes User Manual

User’s Manual
Precise®HEPA-Filtered Glove Boxes
Models Voltage, Frequency 5220200 115V, 60 Hz 5220220 230V, 50 Hz 5220221 230V, 60 Hz 5220230 100V, 50 Hz 5220231 100V, 60 Hz
complete your product registration card
online at register.labconco.com
Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com
Please read the User’s Manual before operating the equipment.
Copyright © 2007, 2008 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of defective material and workmanship, provided such defect results from normal and proper use of the equipment.
The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner, except the following;
Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Lab Animal Research
Stations carry a three-year warranty from date of installation or four years from date of shipment from Labconco, whichever is sooner.
SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from
date of installation or three years from date of shipment from Labconco, whichever is sooner.
Blood Drawing Chairs carry a ten year warranty.
Carts carry a lifetime warranty.
Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages of any kind. This statement may be altered by a specific published amendment. No individual has authorization to alter the provisions of this warranty policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by various federal, state, or local regulations. All users of this equipment are required to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821­5525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #5233702, Rev. A
ECO E766
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: PREREQUISITES 2
Support, Vibration & Preventive Requirements 3 Location Requirements 3 Exhaust and Blower Requirements 3 ISO Class 3 Definition 5 Electrical Requirements 7 Space Requirements 7
CHAPTER 3: GETTING STARTED 8 Unpacking the Glove Box 9 Installing the Glove Box on a Supporting Structure &
Work Surface 10 Connecting to the Exhaust System (If Applicable) 10 Installation of Gloves to the Glove Ports 12 Connecting the Electrical Supply to the Glove Box 13 Setting Exhaust Volume with Speed Control Adjustment 13 Validating the Glove Box 14 Summary of Performance Testing Results 14
CHAPTER 4: PERFORMANCE FEATURES AND SAFETY PRECAUTIONS 16
Performance Features 16 Safety Precautions 19
CHAPTER 5: USING YOUR GLOVE BOX 21 Routine Daily Work Procedures 21
HEPA Filter Applications, Suitability & Guidelines 23 Definition of Terms 24 Appropriate Chemicals for Carbon Filters 24 Hazardous Misapplications for Carbon Filters with Volatile Chemicals 25 Chemical Carcinogen Use with Carbon Filters 25 Prohibited Acid Use 26
O
O
FF
C
C
OONNTTEENNTTSS
CHAPTER 6: MAINTAINING YOUR GLOVE BOX 27
Routine Maintenance Schedule 28
Decontamination 29
Determining When to Replace HEPA Filters 29
How to Install New HEPA Filters 29
HEPA Filter Leak Test 31
Initial Certification 33
Re-Certification 33
Fluorescent Light Replacement 33
Motorized Impeller Replacement 34
Speed Control Replacement 34
CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 35
CHAPTER 8: TROUBLESHOOTING 41
APPENDIX A: REPLACEMENT PARTS 42
APPENDIX B: DIMENSIONS 44
APPENDIX C: SPECIFICATIONS 45
APPENDIX D: IONIZER FAN PERFORMANCE CRITERIA 46
APENDIX E: PRESSURE & FLOW RATE CONVERSIONS 48
APPENDIX F: REFERENCES 49
DECLARATION OF CONFORMITY 50
CChhaapptteerr 11:: IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco Precise® HEPA-Filtered Glove Box. Your glove box provides personnel protection through superior containment rated at Class 1 conditions for hazardous environments, per ISO 10648-2.
These glove boxes will effectively contain toxic or noxious particulates when properly installed and operated. In positive or negative pressure operation modes, the glove box can create an ISO standard Class 3 (ISO 14644-1) clean air environment. This glove box uses two HEPA filters, rated at least 99.99% efficient for 0.3-micron particles.
The Precise HEPA-Filtered Glove Boxes offer many unique features to enhance safety and performance. To take full advantage of them, please acquaint yourself with this manual and keep it handy for future reference. All glove box operators must read and understand how HEPA filtered glove boxes operate; please review Chapter 4: Performance Features and Safety Precautions before beginning to work with this glove box. Even if you are an experienced user, please review Chapter 5: Using Your HEPA-Filtered Glove Box, which describes the Precise features so that you can use the filtered glove box effectively and safely.
Product Service 1-800-522-7658
1
CChhaapptteerr 22:: PPrreerreeqquuiissiitteess
Before you install the glove box, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the glove box. The glove box should be strategically placed in the lab to provide efficient workflow and prevent operator interference from normal traffic patterns.
Carefully read this chapter to learn the requirements for your installation site:
The support, vibration and preventive requirements.
The location requirements.
The exhaust and blower requirements.
The electrical power requirements.
The space requirements.
Refer to Appendix B: Precise HEPA-Filtered Glove Box Dimensions for complete glove box dimensions.
Refer to Appendix C: Precise HEPA-Filtered Glove Box Specifications for complete electrical and environmental conditions, specifications and requirements.
2
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Support, Vibration and Preventive Requirements
In the preparation of a glove box site, please consider the following:
A bench or stand that is rigidly mounted to the floor or fixed to the wall,
but not both, may be appropriate. 35" to 40" (889mm-1016mm) is typical for standing height. (Labconco stands offered in Chapter 7 vary from 33" to 40".)
The corners of a building typically have less vibration than the center,
which promotes analytical balance stability.
The bench typically should not contain any vibration-producing
equipment, such as shakers or pumps.
A marble slab with dampening pads placed within the enclosure is an
effective low cost means of controlling vibration (see Chapter 7: Accessorizing Your Filtered Glove Box).
Location Requirements
The Precise HEPA-Filtered Glove Boxes have been designed to rest on a typical 29"-30" (737mm-762mm) deep work surface. The height should be 35"-40" for standing position. Avoid placing the glove box in high traffic areas where walking might disrupt the operator or experimentation.
Exhaust and Blower Requirements
The Precise HEPA-Filtered Glove Box, as manufactured, uses an integral motorized impeller to pull room air through the inlet and exhaust HEPA filters and discharge the clean air back into the laboratory. This negative pressure operation mode pulls all internally-contaminated air through the exhaust HEPA filter. The HEPA-filtered exhaust air is then forced out the top of the glove box. An optional FilterMate™ Portable Exhauster with a carbon filter may be installed downstream of the exhaust to adsorb low levels of organic fumes, formaldehyde, or ammonia gases.
The HEPA-filtered exhaust air from the glove box can be exhausted to the outside with the installation of a thimble exhaust connection kit and remote blower listed in Chapter 7. As an option, if necessary, the built-in motorized impeller can be wall mounted if desired.
Labconco does not recommend a hard duct connection using the built-in motorized impeller as the source of airflow out of the facility. This creates a positive pressure in the exhaust duct.
Product Service 1-800-522-7658
3
Chapter 2: Prerequisites
Labconco does not recommend hard duct connections with remote blowers due to the air volume balance requirements with such systems. Hard duct connections should only be used when the glove box impeller is disconnected and only a single roof-mounted blower is used. Note that reliance on an external exhaust blower does not provide airflow control at the glove box.
If a positive internal pressure clean air ISO Class 3 condition (ISO 14644-1) is desired, the motorized impeller can be rotated to work in positive pressure mode (see Figure 3-3). See Chapter 7 for the Positive Pressure Conversion Kit. See the following Negative and Positive Pressure Particle Test data in the charts below. Both charts show the dilution rates of the glove boxes.
Precise HEPA-Filtered Glove Box Particle Testing under Negative Pressure
500
NOTE: Ambient Air Baseline Particle Count at 1 Minute 294132 Particles measuring 0.3 Micron detected within 1 Minute. 33296 Particles measuring 0.5 Micron detected within 1 Minute.
Airflow Testing Performed at 50 Dial Setting (61 CFM)
with Glove Box Under Negative Pressure.
> 0.3 Micron
> 0.5 Micron
60
24
0
0
012345678910111213141516171819
14
1 1 1 1
13
11
12
11 11
000 000 00
9
6
4
(Per Cubic Meter Per Minute)
Number of Particles
450
400
350
300
250
200
150
100
50
Time (Minutes)
0
4
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Precise HEPA-Filtered Glove Box Particle Testing under Positive Pressure
500
NOTE: Ambient Air Baseline Particle Count at 1 Minute
450
198671 Particles measuring 0.3 Micron detected within 1 Minute. 25473 Particles measuring 0.5 Micron detected w ithin 1 Minute.
400
350
300
(Per Cubic Meter Per Minute)
250
200
150
100
Number of Particles
50
0
0
0123456
306
Airflow Testing Performed at 50 Dial Setting (61 CFM)
with Glove Box Under Positive Pressure.
139
> 0.3 Micron
> 0.5 Micron
18
12
2
8
1
Time (Minutes)
ISO Class 3 Definition
Airborne particulate cleanliness inside any clean air glove box or enclosure is designated by ISO Class 3, which is equivalent to 35 particles 0.5 µm or larger per cubic meter of air per minute as defined by ISO Standard 14644-1. ISO Class 3 cleanliness is illustrated in the table below and is equivalent to Class 1 air conditions as defined by Federal Standard 209E or l particle 0.5 µm or larger per cubic foot of air per minute.
Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.
ISO
classification
number (N)
ISO Class 1 10 2
Maximum concentration limits (particles/m3 of air/minute) for particles equal to and larger than the considered sizes shown below (concentration limits are calculated in accordance with 3.2 of Standard 14644-1)
0.1 µm 0.2 µm 0.3 µm 0.5 µm 1 µm 5 µm
0
00
ISO Class 2 100 24 10 4
ISO Class 3 1 000 237 102 35 8
ISO Class 4 10 000 2 370 1 020 352 83
ISO Class 5 100 000 23 700 10 200 3 520 832 29
ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293
ISO Class 7 352 000 83 200 2 930
ISO Class 8 3 520 000 832 000 29 300
ISO Class 9 35 200 000 8 320 000 293 000
Table 2-1 ISO Classification Number (N)
Product Service 1-800-522-7658
5
Dial Setting
Min. Dial
20 Dial 40 Dial 50 Dial 60 Dial 80 Dial
Max. 100 Dial
Dial OFF
Chapter 2: Prerequisites
Additionally, the motorized impeller can also be used in a full recirculation mode providing the operator with some control of the atmosphere chemistry within the glove box. See Chapter 7 for the Recirculation Conversion Kit.
The airflow chart below represents typical operational data including air changes per minute, exhaust volume, noise levels, main chamber static pressures, and total system static pressures measured at the blower intake (suction side). Exhaust volumes are reduced by 17% for products operating on 100V, 60 Hz and reduced 34% for 100V, 50 Hz products.
Air Changes
per Minute
(acm)
1
1.5
3.3
4.7
6.2
7.2
7.7 0
Exhaust
Volume
(CFM)
13 20 43 61 80 93
100
0
Noise Pressure
dB (A)
44 48 48 65 68 70 71 37
Static Pressure
Inside Glove Box
(inches of water)
.14 .17 .45 .68 .80 .89 .91
0
Total System
Static Pressure
(inches of water)
.34 .51
1.32
1.81
2.14
2.38
2.42 0
PRECISE HEPA-FILTERED GLOVE BOX
2.5
Static Pressure Inside Glove Box
2
1.5
1
(Inches of Water Column)
Total Static Pressure at Blower Intake (Suction Side)
Negative Static Pressure
0.5
0.3435
0
10 20 30 40 50 60 70 80 90 100
0.14
0.5072
0.17
Static Pressure Test Results
1.81
1.323
0.68
0.45
Airflow Volume
(Cubic Feet per Minute)
2.141
0.8
2.375
0.89
2.422
0.91
6
Product Service 1-800-522-7658
!
Chapter 2: Prerequisites
For thimble-ducted glove boxes, the proper remote blower selection can be determined from these exhaust requirements as the remote blower only needs to be sized for the static pressure loss of the ductwork system. The thimble ducted exhaust blower should be sized for 25% additional airflow. When exhausting a glove box to the outside, it should be connected to a dedicated remote blower.
Labconco offers accessory remote blowers listed in Chapter 7. Contact Labconco for blower sizing assistance.
When a glove box is connected to a remote blower exhaust system, an adjustable damper (or valve) should be installed in the duct to control the airflow volumes properly. See Chapter 7 for Exhaust Dampers.
Electrical Requirements
Electrical service should be nearby for connecting the glove box, and accessory equipment. The glove box is rated 115V, 8A or 230V, 4A or 100V, 8A. A convenience duplex outlet is located inside the glove box and is rated for 115V, 5A or 230V, 2A or 100V, 5A. Additional power strip accessories are available for connection to the duplex outlet and are listed in Chapter 7.
Space Requirements
The dimensions are shown in Appendix B: Dimensions.
Product Service 1-800-522-7658
7
CChhaapptteerr 33:: GGeettttiinngg SSttaarrtteedd
Once the site for your Precise Glove Box is properly prepared, you are ready to unpack, inspect, install, and validate your system. Read this chapter to learn how to:
Unpack and move the glove box.
Set up the glove box with the proper supporting structure and work
surface.
Connect to an exhaust system if applicable.
Install the gloves.
Connect the electrical supply.
Set the exhaust volume with the speed control adjustment.
Arrange validation for the glove box.
Each Precise Glove Box weighs 205 lbs. (90 kg). The
!
shipping container allows for lifting with a mechanical lift truck or floor jack. If you must lift the enclosure manually, follow safe-lifting guidelines. Do not lift by the upper sheet metal façade and front panel as damage can occur to the plastic glove box liner (see Figure 3-1).
8
Product Service 1-800-522-7658
Chapter 3: Getting Started
Figure 3-1
Lifting Instructions
Unpacking the Glove Box
We recommend that you do not remove the glove box from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack.
Carefully remove the carton shrink-wrap on the glove box and inspect it for damage that may have occurred in transit. If damaged, notify the delivery
carrier immediately and retain the entire shipment intact for inspection by the carrier.
THE UNITED STATES INTERSTATE COMMERCE COMMISSION RULES REQUIRE THAT CLAIMS BE
)
)
FILED WITH THE DELIVERY CARRIER WITHIN FIFTEEN (15) DAYS OF DELIVERY.
DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED.
Product Service 1-800-522-7658
9
Chapter 3: Getting Started
IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT
)
Do not discard the packing material until you have checked all of the components and tested the glove box.
CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES.
Installing the Glove Box on a Supporting Structure and Work Surface
Exercise caution when lifting or moving the glove box.
When installing the glove box onto a work surface or benchtop, ensure that the structure can safely support the combined weight of the glove box and any related equipment. The work surface should be as wide as the glove box to properly support it. The front of the glove box should be aligned with the front of the work surface for optimal comfort. A height of 35"-40" (889mm­1016mm) is appropriate for standing operation. Adjustable Height Base Stands listed in Chapter 7 may be adjusted from 33"-40" (838mm-1016mm).
Connecting to the Exhaust System (If Applicable)
WARNING: The weight of any exhaust ductwork system
!
The Precise HEPA-Filtered Glove Box has been manufactured to exhaust into the laboratory in its standard configuration. To remove chemical fumes and odors not trapped by the HEPA filter, the glove box can be exhausted to the outside, or connected to an accessory FilterMate Portable Exhauster with carbon filters (see Figure 3-2). See Chapter 7 for accessories including: Thimble Exhaust Connection, FilterMate Portable Exhauster, Positive Pressure Conversion Kit, Recirucation Kit, Hard Duct Connection.
Consult Labconco Customer Service if you require help sizing a remote blower for the exhaust volume and the duct work system (static pressure loss listed in Chapter 2).
!
10
must be supported independently of the glove box superstructure or damage may occur.
The exhaust system should be installed by a qualified HVAC contractor.
To ensure compatibility, the selected exhaust duct material should match the enclosure, procedures and chemical applications.
Product Service 1-800-522-7658
Chapter 3: Getting Started
Exhaust Connection to Outside
(All dimensions in inches)
Product Service 1-800-522-7658
Figure 3-2
11
Chapter 3: Getting Started
Positive Pressure Glove Box Configuration
Figure 3-3
Installation of Gloves to the Glove Ports
With thumbs up and right/left orientation, secure the gloves in place on the glove ports by stretching the beaded cuff into the groove nearest the window. Install the separate 8" diameter O-ring over the gloves and into the outer groove of the glove port surface. Stainless steel band clamps are provided for securing the separate O-ring into the glove port groove. Replacement gloves and parts are listed in Appendix A with a description in Chapter 7.
12
Figure 3-4
Glove Installation
Product Service 1-800-522-7658
Chapter 3: Getting Started
Connecting the Electrical Supply to the Glove Box
115V, 60 Hz; 100V, 50 Hz; or 100V, 60 Hz Models
Connect the power cord supplied to the IEC electrical supply plug on the back of the glove box. 115V or 100V models are rated at 8 amps total.
The maximum circuit load for the interior electrical duplex is 5 amps.
230V, 50 Hz or 230V, 60 Hz Models
The same procedure applies for the 230V model except it is shipped without a plug. Install the appropriate plug per local requirements. The 230V, 50 Hz or 230V, 60 Hz models are both rated at 4 amps total. The interior
electrical duplex is rated at 2 amps.
Setting the Exhaust Volume with the Speed Control Adjustment
For Precise HEPA-Filtered Glove Boxes, adjustment of the speed control provides control of exhaust air volume and is located on the front panel. The exhaust air volume should be adjusted at the low setting for weighing operations. (Consult your Safety Officer for airflow recommendations for your application). Working at the lowest exhaust volume appropriate for the application will give the quietest operation. To increase the rate of air volume changes after working in the glove box and to ensure proper cleaning, the speed control should be set at a higher (50 to 100) dial setting for 5 to 10 minutes as recommended by the operator’s predetermined protocol and the chart below. The exhaust volume is increased by turning the speed control counterclockwise.
Minimum
Dial Setting
for Cleaning
Internal Air
Volume
50 4.7 61 10
100 7.7 100 5
Approximate
Air Volume
Changes per
Minute
Estimated
Total Exhaust
Volume
(CFM)
Recommended
Times to
Clean Internal
Air Volume
(minutes)
Product Service 1-800-522-7658
13
Loading...
+ 37 hidden pages