Labconco Precise HEPA-Filtered Glove Boxes User Manual
User’s Manual
Precise®HEPA-Filtered
Glove Boxes
Models Voltage, Frequency
5220200 115V, 60 Hz
5220220 230V, 50 Hz
5220221 230V, 60 Hz
5220230 100V, 50 Hz
5220231 100V, 60 Hz
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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com
Please read the User’s Manual before operating the equipment.
Copyright © 2007, 2008 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all
rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic
design changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
• Purifier® Delta® Series Biological Safety Cabinets and PuriCare® Lab Animal Research
Stations carry a three-year warranty from date of installation or four years from date of
shipment from Labconco, whichever is sooner.
• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from
date of installation or three years from date of shipment from Labconco, whichever is
sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair
or replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement
may be altered by a specific published amendment. No individual has authorization to alter the
provisions of this warranty policy or its amendments. Lamps and filters are not covered by this
warranty. Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight
carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the
delivery carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed
by various federal, state, or local regulations. All users of this equipment are required to become
familiar with any regulations that apply in the user’s area concerning the dumping of waste materials
in or upon water, land, or air and to comply with such regulations. Labconco Corporation is held
harmless with respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #5233702, Rev. A
ECO E766
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: PREREQUISITES 2
Support, Vibration & Preventive Requirements 3
Location Requirements 3
Exhaust and Blower Requirements 3
ISO Class 3 Definition 5
Electrical Requirements 7
Space Requirements 7
CHAPTER 3: GETTING STARTED 8
Unpacking the Glove Box 9
Installing the Glove Box on a Supporting Structure &
Work Surface 10
Connecting to the Exhaust System (If Applicable) 10
Installation of Gloves to the Glove Ports 12
Connecting the Electrical Supply to the Glove Box 13
Setting Exhaust Volume with Speed Control Adjustment 13
Validating the Glove Box 14
Summary of Performance Testing Results 14
CHAPTER 4: PERFORMANCE FEATURES AND
SAFETY PRECAUTIONS 16
Performance Features 16
Safety Precautions 19
CHAPTER 5: USING YOUR GLOVE BOX 21
Routine Daily Work Procedures 21
HEPA Filter Applications, Suitability & Guidelines 23
Definition of Terms 24
Appropriate Chemicals for Carbon Filters 24
Hazardous Misapplications for Carbon Filters with
Volatile Chemicals 25
Chemical Carcinogen Use with Carbon Filters 25
Prohibited Acid Use 26
O
O
FF
C
C
OONNTTEENNTTSS
CHAPTER 6: MAINTAINING YOUR GLOVE BOX 27
Routine Maintenance Schedule 28
Decontamination 29
Determining When to Replace HEPA Filters 29
How to Install New HEPA Filters 29
HEPA Filter Leak Test 31
Initial Certification 33
Re-Certification 33
Fluorescent Light Replacement 33
Motorized Impeller Replacement 34
Speed Control Replacement 34
CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 35
CHAPTER 8: TROUBLESHOOTING 41
APPENDIX A: REPLACEMENT PARTS 42
APPENDIX B: DIMENSIONS 44
APPENDIX C: SPECIFICATIONS 45
APPENDIX D: IONIZER FAN PERFORMANCE CRITERIA 46
APENDIX E: PRESSURE & FLOW RATE CONVERSIONS 48
APPENDIX F: REFERENCES 49
DECLARATION OF CONFORMITY 50
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on your purchase of a Labconco Precise® HEPA-Filtered
Glove Box. Your glove box provides personnel protection through superior
containment rated at Class 1 conditions for hazardous environments, per
ISO 10648-2.
These glove boxes will effectively contain toxic or noxious particulates when
properly installed and operated. In positive or negative pressure operation
modes, the glove box can create an ISO standard Class 3 (ISO 14644-1) clean
air environment. This glove box uses two HEPA filters, rated at least 99.99%
efficient for 0.3-micron particles.
The Precise HEPA-Filtered Glove Boxes offer many unique features to
enhance safety and performance. To take full advantage of them, please
acquaint yourself with this manual and keep it handy for future reference.
All glove box operators must read and understand how HEPA filtered glove
boxes operate; please review Chapter 4: Performance Features and Safety
Precautions before beginning to work with this glove box. Even if you are
an experienced user, please review Chapter 5: Using Your HEPA-Filtered
Glove Box, which describes the Precise features so that you can use the
filtered glove box effectively and safely.
Product Service 1-800-522-7658
1
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install the glove box, you need to prepare your site for
installation. You must be certain that the area is level and of solid
construction. In addition, a dedicated source of electrical power should be
located near the installation site to power the glove box. The glove box
should be strategically placed in the lab to provide efficient workflow and
prevent operator interference from normal traffic patterns.
Carefully read this chapter to learn the requirements for your installation site:
• The support, vibration and preventive requirements.
• The location requirements.
• The exhaust and blower requirements.
• The electrical power requirements.
• The space requirements.
Refer to Appendix B: Precise HEPA-Filtered Glove Box Dimensions for
complete glove box dimensions.
Refer to Appendix C: Precise HEPA-Filtered Glove Box Specifications for
complete electrical and environmental conditions, specifications and
requirements.
2
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Support, Vibration and Preventive Requirements
In the preparation of a glove box site, please consider the following:
• A bench or stand that is rigidly mounted to the floor or fixed to the wall,
but not both, may be appropriate. 35" to 40" (889mm-1016mm) is
typical for standing height. (Labconco stands offered in Chapter 7 vary
from 33" to 40".)
• The corners of a building typically have less vibration than the center,
which promotes analytical balance stability.
• The bench typically should not contain any vibration-producing
equipment, such as shakers or pumps.
• A marble slab with dampening pads placed within the enclosure is an
effective low cost means of controlling vibration (see Chapter 7:
Accessorizing Your Filtered Glove Box).
Location Requirements
The Precise HEPA-Filtered Glove Boxes have been designed to rest on a
typical 29"-30" (737mm-762mm) deep work surface. The height should be
35"-40" for standing position. Avoid placing the glove box in high traffic
areas where walking might disrupt the operator or experimentation.
Exhaust and Blower Requirements
The Precise HEPA-Filtered Glove Box, as manufactured, uses an integral
motorized impeller to pull room air through the inlet and exhaust HEPA
filters and discharge the clean air back into the laboratory. This negative
pressure operation mode pulls all internally-contaminated air through the
exhaust HEPA filter. The HEPA-filtered exhaust air is then forced out the
top of the glove box. An optional FilterMate™ Portable Exhauster with a
carbon filter may be installed downstream of the exhaust to adsorb low levels
of organic fumes, formaldehyde, or ammonia gases.
The HEPA-filtered exhaust air from the glove box can be exhausted to the
outside with the installation of a thimble exhaust connection kit and remote
blower listed in Chapter 7. As an option, if necessary, the built-in motorized
impeller can be wall mounted if desired.
Labconco does not recommend a hard duct connection using the built-in
motorized impeller as the source of airflow out of the facility. This
creates a positive pressure in the exhaust duct.
Product Service 1-800-522-7658
3
Chapter 2: Prerequisites
Labconco does not recommend hard duct connections with remote
blowers due to the air volume balance requirements with such systems.
Hard duct connections should only be used when the glove box impeller
is disconnected and only a single roof-mounted blower is used. Note that
reliance on an external exhaust blower does not provide airflow control
at the glove box.
If a positive internal pressure clean air ISO Class 3 condition (ISO 14644-1)
is desired, the motorized impeller can be rotated to work in positive pressure
mode (see Figure 3-3). See Chapter 7 for the Positive Pressure Conversion
Kit. See the following Negative and Positive Pressure Particle Test data in
the charts below. Both charts show the dilution rates of the glove boxes.
Precise HEPA-Filtered Glove Box Particle Testing under Negative Pressure
500
NOTE: Ambient Air Baseline Particle Count at 1 Minute
294132 Particles measuring 0.3 Micron detected within 1 Minute.
33296 Particles measuring 0.5 Micron detected within 1 Minute.
Airflow Testing Performed at 50 Dial Setting (61 CFM)
with Glove Box Under Negative Pressure.
> 0.3 Micron
> 0.5 Micron
60
24
0
0
012345678910111213141516171819
14
1 1 1 1
13
11
12
11 11
000 000 00
9
6
4
(Per Cubic Meter Per Minute)
Number of Particles
450
400
350
300
250
200
150
100
50
Time (Minutes)
0
4
Product Service 1-800-522-7658
Chapter 2: Prerequisites
Precise HEPA-Filtered Glove Box Particle Testing under Positive Pressure
500
NOTE: Ambient Air Baseline Particle Count at 1 Minute
450
198671 Particles measuring 0.3 Micron detected within 1 Minute.
25473 Particles measuring 0.5 Micron detected w ithin 1 Minute.
400
350
300
(Per Cubic Meter Per Minute)
250
200
150
100
Number of Particles
50
0
0
0123456
306
Airflow Testing Performed at 50 Dial Setting (61 CFM)
with Glove Box Under Positive Pressure.
139
> 0.3 Micron
> 0.5 Micron
18
12
2
8
1
Time (Minutes)
ISO Class 3 Definition
Airborne particulate cleanliness inside any clean air glove box or enclosure is
designated by ISO Class 3, which is equivalent to 35 particles 0.5 µm or
larger per cubic meter of air per minute as defined by ISO Standard 14644-1.
ISO Class 3 cleanliness is illustrated in the table below and is equivalent to
Class 1 air conditions as defined by Federal Standard 209E or l particle 0.5
µm or larger per cubic foot of air per minute.
Table 1-1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones.
ISO
classification
number (N)
ISO Class 1 10 2
Maximum concentration limits (particles/m3 of air/minute) for particles equal to
and larger than the considered sizes shown below (concentration limits are
calculated in accordance with 3.2 of Standard 14644-1)
0.1 µm 0.2 µm 0.3 µm 0.5 µm 1 µm 5 µm
0
00
ISO Class 2 100 24 10 4
ISO Class 3 1 000 237 102 35 8
ISO Class 4 10 000 2 370 1 020 352 83
ISO Class 5 100 000 23 700 10 200 3 520 832 29
ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293
ISO Class 7 352 000 83 200 2 930
ISO Class 8 3 520 000 832 000 29 300
ISO Class 9 35 200 000 8 320 000 293 000
Table 2-1 ISO Classification Number (N)
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5
Dial Setting
Min. Dial
20 Dial
40 Dial
50 Dial
60 Dial
80 Dial
Max. 100 Dial
Dial OFF
Chapter 2: Prerequisites
Additionally, the motorized impeller can also be used in a full recirculation
mode providing the operator with some control of the atmosphere chemistry
within the glove box. See Chapter 7 for the Recirculation Conversion Kit.
The airflow chart below represents typical operational data including air
changes per minute, exhaust volume, noise levels, main chamber static
pressures, and total system static pressures measured at the blower intake
(suction side). Exhaust volumes are reduced by 17% for products operating
on 100V, 60 Hz and reduced 34% for 100V, 50 Hz products.
Air Changes
per Minute
(acm)
1
1.5
3.3
4.7
6.2
7.2
7.7
0
Exhaust
Volume
(CFM)
13
20
43
61
80
93
100
0
Noise Pressure
dB (A)
44
48
48
65
68
70
71
37
Static Pressure
Inside Glove Box
(inches of water)
.14
.17
.45
.68
.80
.89
.91
0
Total System
Static Pressure
(inches of water)
.34
.51
1.32
1.81
2.14
2.38
2.42
0
PRECISE HEPA-FILTERED GLOVE BOX
2.5
Static Pressure Inside Glove Box
2
1.5
1
(Inches of Water Column)
Total Static Pressure at Blower
Intake (Suction Side)
Negative Static Pressure
0.5
0.3435
0
10 20 30 40 50 60 70 80 90 100
0.14
0.5072
0.17
Static Pressure Test Results
1.81
1.323
0.68
0.45
Airflow Volume
(Cubic Feet per Minute)
2.141
0.8
2.375
0.89
2.422
0.91
6
Product Service 1-800-522-7658
!
Chapter 2: Prerequisites
For thimble-ducted glove boxes, the proper remote blower selection can be
determined from these exhaust requirements as the remote blower only needs
to be sized for the static pressure loss of the ductwork system. The thimble
ducted exhaust blower should be sized for 25% additional airflow. When
exhausting a glove box to the outside, it should be connected to a dedicated
remote blower.
Labconco offers accessory remote blowers listed in Chapter 7. Contact
Labconco for blower sizing assistance.
When a glove box is connected to a remote blower exhaust system, an
adjustable damper (or valve) should be installed in the duct to control the
airflow volumes properly. See Chapter 7 for Exhaust Dampers.
Electrical Requirements
Electrical service should be nearby for connecting the glove box, and
accessory equipment. The glove box is rated 115V, 8A or 230V, 4A or
100V, 8A. A convenience duplex outlet is located inside the glove box and is
rated for 115V, 5A or 230V, 2A or 100V, 5A. Additional power strip
accessories are available for connection to the duplex outlet and are listed in
Chapter 7.
Space Requirements
The dimensions are shown in Appendix B: Dimensions.
Product Service 1-800-522-7658
7
CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Once the site for your Precise Glove Box is properly prepared, you are ready
to unpack, inspect, install, and validate your system. Read this chapter to
learn how to:
• Unpack and move the glove box.
• Set up the glove box with the proper supporting structure and work
surface.
• Connect to an exhaust system if applicable.
• Install the gloves.
• Connect the electrical supply.
• Set the exhaust volume with the speed control adjustment.
• Arrange validation for the glove box.
Each Precise Glove Box weighs 205 lbs. (90 kg). The
!
shipping container allows for lifting with a mechanical lift
truck or floor jack. If you must lift the enclosure manually,
follow safe-lifting guidelines. Do not lift by the upper sheet
metal façade and front panel as damage can occur to the
plastic glove box liner (see Figure 3-1).
8
Product Service 1-800-522-7658
Chapter 3: Getting Started
Figure 3-1
Lifting Instructions
Unpacking the Glove Box
We recommend that you do not remove the glove box from its shipping
container until it is ready to be placed into its final location. Move the unit
by placing a flat, low dolly under the shipping skid, or by using a floor jack.
Carefully remove the carton shrink-wrap on the glove box and inspect it for
damage that may have occurred in transit. If damaged, notify the delivery
carrier immediately and retain the entire shipment intact for inspection
by the carrier.
THE UNITED STATES INTERSTATE COMMERCE
COMMISSION RULES REQUIRE THAT CLAIMS BE
)
)
FILED WITH THE DELIVERY CARRIER WITHIN
FIFTEEN (15) DAYS OF DELIVERY.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
Product Service 1-800-522-7658
9
Chapter 3: Getting Started
IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU
MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT
)
Do not discard the packing material until you have checked all of the
components and tested the glove box.
CARRIER. LABCONCO CORPORATION AND ITS
DEALERS ARE NOT RESPONSIBLE FOR SHIPPING
DAMAGES.
Installing the Glove Box on a Supporting
Structure and Work Surface
Exercise caution when lifting or moving the glove box.
When installing the glove box onto a work surface or benchtop, ensure that
the structure can safely support the combined weight of the glove box and
any related equipment. The work surface should be as wide as the glove box
to properly support it. The front of the glove box should be aligned with the
front of the work surface for optimal comfort. A height of 35"-40" (889mm1016mm) is appropriate for standing operation. Adjustable Height Base
Stands listed in Chapter 7 may be adjusted from 33"-40" (838mm-1016mm).
Connecting to the Exhaust System (If Applicable)
WARNING: The weight of any exhaust ductwork system
!
The Precise HEPA-Filtered Glove Box has been manufactured to exhaust
into the laboratory in its standard configuration. To remove chemical fumes
and odors not trapped by the HEPA filter, the glove box can be exhausted to
the outside, or connected to an accessory FilterMate Portable Exhauster with
carbon filters (see Figure 3-2). See Chapter 7 for accessories including:
Thimble Exhaust Connection, FilterMate Portable Exhauster, Positive
Pressure Conversion Kit, Recirucation Kit, Hard Duct Connection.
Consult Labconco Customer Service if you require help sizing a remote
blower for the exhaust volume and the duct work system (static pressure loss
listed in Chapter 2).
!
10
must be supported independently of the glove box
superstructure or damage may occur.
The exhaust system should be installed by a qualified
HVAC contractor.
To ensure compatibility, the selected exhaust duct material
should match the enclosure, procedures and chemical
applications.
Product Service 1-800-522-7658
Chapter 3: Getting Started
Exhaust Connection to Outside
(All dimensions in inches)
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Figure 3-2
11
Chapter 3: Getting Started
Positive Pressure Glove Box Configuration
Figure 3-3
Installation of Gloves to the Glove Ports
With thumbs up and right/left orientation, secure the gloves in place on the
glove ports by stretching the beaded cuff into the groove nearest the window.
Install the separate 8" diameter O-ring over the gloves and into the outer
groove of the glove port surface. Stainless steel band clamps are provided for
securing the separate O-ring into the glove port groove. Replacement gloves
and parts are listed in Appendix A with a description in Chapter 7.
12
Figure 3-4
Glove Installation
Product Service 1-800-522-7658
Chapter 3: Getting Started
Connecting the Electrical Supply to the Glove Box
115V, 60 Hz; 100V, 50 Hz; or 100V, 60 Hz Models
Connect the power cord supplied to the IEC electrical supply plug on the
back of the glove box. 115V or 100V models are rated at 8 amps total.
The maximum circuit load for the interior electrical duplex is 5 amps.
230V, 50 Hz or 230V, 60 Hz Models
The same procedure applies for the 230V model except it is shipped without
a plug. Install the appropriate plug per local requirements. The 230V, 50 Hz
or 230V, 60 Hz models are both rated at 4 amps total. The interior
electrical duplex is rated at 2 amps.
Setting the Exhaust Volume with the Speed
Control Adjustment
For Precise HEPA-Filtered Glove Boxes, adjustment of the speed control
provides control of exhaust air volume and is located on the front panel. The
exhaust air volume should be adjusted at the low setting for weighing
operations. (Consult your Safety Officer for airflow recommendations
for your application). Working at the lowest exhaust volume appropriate
for the application will give the quietest operation. To increase the rate of air
volume changes after working in the glove box and to ensure proper cleaning,
the speed control should be set at a higher (50 to 100) dial setting for 5 to 10
minutes as recommended by the operator’s predetermined protocol and the
chart below. The exhaust volume is increased by turning the speed control
counterclockwise.
Minimum
Dial Setting
for Cleaning
Internal Air
Volume
50 4.7 61 10
100 7.7 100 5
Approximate
Air Volume
Changes per
Minute
Estimated
Total Exhaust
Volume
(CFM)
Recommended
Times to
Clean Internal
Air Volume
(minutes)
Product Service 1-800-522-7658
13
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