Labconco Biological Safety Cabinets 34609 User Manual
User’s Manual
Purifier®CELL Series
Biological Safety Cabinets
Models
34309 Series 34509 Series
34409 Series 34609 Series
TYPE
A2
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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX 816-363-0130
E-MAIL labconco@labconco.com
HOME PAGE www.labconco.com
Please read the User’s Manual before operating the equipment.
Copyright © 2009 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;
• Purifier® Logic® and Cell Logic® Biological Safety Cabinets and PuriCare® Lab Animal
Research Stations carry a three-year warranty from date of installation or four years from date
of shipment from Labconco, whichever is sooner.
• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date
of installation or three years from date of shipment from Labconco, whichever is sooner.
• Blood Drawing Chairs carry a ten year warranty.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight
carrier. Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar
with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon
water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with
respect to user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-8215525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #4028900, Rev. B
ECO F719
T
AABBLLEE
T
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: PREREQUISITES 2
Space Requirements 2
Clearance 2
Location Requirements 3
Exhaust Requirements 4
Electrical Requirements 4
Service Line Requirements 5
User-Supplied Microscope Requirements 5
User-Supplied Circulator Requirements 6
CHAPTER 3: GETTING STARTED 7
Unpacking the Biosafety Cabinet 8
Moving the Cabinet 8
Preparing the Biosafety Cabinet for Operation 9
Installing the Biosafety Cabinet on an Existing Work Surface 9
Installing the Cabinet on a Labconco Base Stand 9
Telescoping Base Stands 9
Manual or Electric Hydraulic Lift Base Stands 11
Connecting the Biosafety Cabinet to Utility Service Lines 11
Optional Exhaust System Connections 12
Drain Valve Installation 13
Microscope Installation (optional) 14
Circulator Installation (optional) 16
Initial Certification 18
CHAPTER 4: PERFORMANCE FEATURES AND SAFETY
HEPA Filters 19
ULPA Filters 20
Laminar Airflow 21
Directional Airflow 22
Motor/Blower 23
Cabinet Air Intakes (Grilles), Ductwork & Air Balance Controls 24
O
O
PRECAUTIONS 19
FF
C
C
OONNTTEENNTTSS
Ultraviolet (UV) Lamp 24
Scope-Ready Package Option 25
Temp-Zone Package Option 25
Safety Precautions 26
CHAPTER 5: USING THE CABINET 28
System Reset Switch 28
Information Center 29
Status Area 29
Data Area 30
Icon Area 30
Operating the Sliding Sash 31
Starting the Biosafety Cabinet 32
The Logic Touchpad 33
User Selectable Features 38
Timer Operation 38
Interval Timer Operation 39
Stopwatch Timer Operation 39
Menu Options 39
UV Lamp Operation 39
UV Lamp Timer 40
UV Lamp Maintenance 40
Light Operation 41
Light Timer 41
Light Maintenance 41
Blower Operation 42
Cabinet Set Up 42
If An Airflow Alert Activates 45
Resetting the Airflow Alert System 45
Working in the Biosafety Cabinet 45
CHAPTER 6: MAINTAINING THE CABINET 48
Routine Maintenance Schedule 48
Service Operations 49
Work Surface Removal 49
Front Grille Removal 49
Towel Catch Removal 50
Front Panel Removal & Installation 51
Changing the Fluorescent Lamps 52
Changing the Optional UV Lamp 52
Resetting a Circuit Breaker 53
Storage 53
CHAPTER 7: TROUBLESHOOTING 54
APPENDIX A: COMPONENTS 57
APPENDIX B: DIMENSIONS 59
APPENDIX C: SPECIFICATIONS 61
Electrical Data 61
Motor Specifications 61
Environmental Conditions 62
APPENDIX D: ACCESSORIES 63
Telescoping Base Stands 63
Telescoping Base Stand Shelf Kit 63
Manual or Electric Hydraulic Lift Base Stands 63
Hydraulic Base Stand Shelf Kit 64
SoLo Electric Hydraulic Lift Base Stands 64
Seismic Bracket Kit 64
Service Valve Kit 64
Ultraviolet Lamp Kits 64
IV Bar Kits 65
Shelf 65
Cord/Tubing Management System 65
Prefilter 65
Postfilter 65
Postfilter Conversion Kit 66
Canopy Connection Kit 66
Air-Tight Damper 66
Backdraft Damper 66
Remote Blowers 66
Ergonomic Chair with Armrests 67
Adjustable Footrest 67
Inflow/Downflow Sensor Kit 67
Aspirator Pump Kit 67
Turntable 67
ULPA Supply Filters 67
ULPA Exhaust Filters 67
Pure-Vu Air Barrier Seals 68
Stand-Still Isolation Platforms 69
Tube Racks/Blocks/Holders, Circulator Shelf,
Connectors and Tubing 69
APPENDIX E: QUICK CHART 70
APPENDIX F: INSTALLATION GUIDE FOR ALL PURE-VU
SEALS AND STAND-STILL ISOLATION
PLATFORMS 71
DECLARATION OF CONFORMITY 80
CChhaapptteerr 11::
IInnttrroodduuccttiioonn
Congratulations on the purchase of a Labconco Purifier® Cell Logic
Biosafety Cabinet. The Biosafety Cabinet is designed to protect you, the
product and the laboratory environment from biohazardous aerosols. The
Cell Logic Series Cabinet is the result of users like you requiring a customer
supplied microscope integrated into the cabinet and a temperature controlled
work area to maintain sample temperature. The Cell Logic Biosafety Cabinet
is available with either or both Scope-Ready™ and Temp-Zone™ package
options.
The Biosafety Cabinet offers many unique features to enhance safety,
performance and ergonomics. To take full advantage of them, please
acquaint yourself with this manual and keep it handy for future reference. If
you are unfamiliar with how biosafety cabinets operate, please review
Chapter 4: Performance Features and Safety Precautions before you begin
working in the cabinet. Even if you are an experienced biosafety cabinet
user, please review Chapter 5: Using the Cabinet; it describes the Biosafety
Cabinet’s features so that you can use it efficiently.
This manual and other technical information is available in PDF format
at our website: www.labconco.com.
®
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1
CChhaapptteerr 22::
PPrreerreeqquuiissiitteess
Before you install the Biosafety Cabinet, you need to prepare the site for
installation. Examine the location where you intend to install the cabinet.
You must be certain that the area is level and of solid construction. In
addition, a dedicated source of electrical power must be located near the
installation site.
Carefully read this chapter to learn:
• Location requirements.
• Electrical power requirements.
• Exhaust requirements.
• Service utility requirements.
• Space requirements.
• User-supplied microscope requirements (if needed)
• User-supplied circulator requirements (if needed)
Refer to Appendix C: Specifications, for complete biosafety cabinet electrical
and environmental conditions, specifications and requirements.
Space Requirements
The dimensions for the Cell Logic A2 Biosafety Cabinets are shown in Appendix
B: Dimensions.
Clearance
A minimum clearance of at least 6 inches (150 mm) is suggested on the top
and both sides of the cabinet for service.
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Chapter 2: Prerequisites
Location Requirements
Note: The Biosafety Cabinet should be located away from traffic patterns,
doors, fans, ventilation registers, fume hoods and any other air-handling
devices that could disrupt its airflow patterns. All windows in the room
should remain closed. Figure 2-1 shows the preferred location for the
Biosafety Cabinet.
Preferred location
Air register blocked or redirected
to prevent cabinet disruption
Figure 2-1
Alternate location
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Chapter 2: Prerequisites
Exhaust Requirements
If you intend to connect the Biosafety Cabinet to the optional Canopy
Connection Kit, first examine the location to ensure that it accommodates the
cabinet’s exhaust duct. The area directly above the cabinet’s exhaust port
should be clear of structural elements, water and utility lines, or other fixed
obstructions. There should be enough clearance to accommodate a 10-inch
diameter duct. Avoid cabinet locations that require an elbow directly above
the cabinet’s exhaust connection or an excessive number of elbows in the
exhaust system. For further information about the cabinet’s exhaust system
requirements, please refer to Chapter 3: Getting Started.
Electrical Requirements
The different Biosafety Cabinet models have the following electrical
requirements:
Voltage
and Size Base Model Last Model Digits Requirements
115V
3'
4'
5'
6'
100V
3'
4'
5'
6'
230V
3'
4'
5'
6'
34309-xx
34409-xx
34509-xx
34609-xx
34309-xx
34409-xx
34509-xx
34609-xx
34309-xx
34409-xx
34509-xx
34609-xx
115 VAC, 60 Hz, 12 Amps
-30 to 31, -60 to 61, -80 to 81
115 VAC, 60 Hz, 16 Amps
100 VAC, 50/60 Hz, 12 Amps
-32 to 33, -62 to 63, -82 to 83
100 VAC, 50/60 Hz, 16 Amps
230 VAC, 50/60 Hz, 6 Amps
-34 to 43, -64 to 73, -84 to 93
230 VAC, 50/60 Hz, 8 Amps
Table 2-1
Note: A dedicated outlet with a circuit breaker rated at 20 amps should
be located as close as possible to the right rear side of the cabinet, at a
height even with, or higher than, the top of the bench or stand.
Note: On 115 VAC models, both electrical outlets are protected by a ground
fault interrupter circuit (GFIC). Labconco does not recommend plugging the
Biosafety Cabinet into a GFIC outlet.
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Chapter 2: Prerequisites
Service Line Requirements
All utility service lines should be ¼ inch O.D., brass, copper, or stainless
steel, and equipped with an easily accessible shut-off valve. If the service
line pressure exceeds 100 PSI, it must be equipped with a pressure regulator
to reduce the line pressure.
Note: The use of flammable gases or solvents should be avoided in the
Biosafety Cabinet. Open flame in the cabinet will disrupt the laminar airflow
in the cabinet and may damage the HEPA filters. Flammable gases or
solvents may reach explosive concentrations in the cabinet or ductwork. If
you feel that the procedure requires the use of an open flame or flammable
materials, contact your institution’s safety office.
The use of air or gases under high pressure should be avoided as they may
seriously disrupt the airflow patterns in the cabinet.
User-Supplied Microscope Requirements –
Purifier Cell Logic Biosafety Cabinets with
Scope-Ready™ Package Only
If installing a microscope, locate the Stand-Still™ Isolation Platform in a
separate box shipped with the cabinet. The Stand-Still Isolation Platform
supports the microscope on top of the stainless steel work surface to
eliminate up to 94% of vibrations and to promote safe airflow. If using a
stereoscope with a forward extending base, the extended Stand-Still XL
Isolation Platform should be ordered from Appendix D: Accessories. Figures
from Appendix F should be used to assist your set-up.
On models with the Scope-Ready package, the Cell Logic Biosafety Cabinet
includes a clear, disposable Pure-Vu™ Seal that can be altered to
accommodate most microscope’s eyepieces. Utilize the Punch Kit
(#4027801), supplied with your cabinet, to cut the two eyepiece holes at
approximately 2.75" (69.9 mm) spacing to match the height of your scope.
As a convenience, pre-cut Pure-Vu eyepiece seals can be ordered from
Appendix D: Accessories. The pre-cut accessory Pure-Vu Seals are based off
the nominal height from the base of the microscope to eyepiece centerline
and can save you valuable time modifying the seal shipped with the Cell
Logic. If using a stereoscope with a forward extending base, one of the PureVu XL Seals should be ordered from Appendix D: Accessories. Figures from
Appendix F should be used to assist your set-up for all Pure-Vu Seals.
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Chapter 2: Prerequisites
User Supplied Circulator Requirements – Purifier
Cell Logic Biosafety Cabinets with Temp Zone™
Package Only
The Temp Zone™ features a temperature controlled area on the work surface
where heated or chilled samples may be transferred and kept at optimum
temperature. A user-supplied circulator that continually delivers heated or
chilled water at a minimum flow rate of 0.5 GPM (1.9 LPM) with typical
volume of 6 liters is required and may be obtained from your laboratory
supply dealer. See Figure 3-6 in Chapter 3.
Quick connection fittings are included on the left side of the cabinet and are
sized for 3/8" diameter polyethylene tubing. As a convenience, an ample
supply (20'; 6 m) of 3/8" (9.5 mm) diameter tubing, insulation, and quick
connect fittings (3/8 tube x 1/8 NPT, 3/8 tube x 1/4 NPT, 3/8 tube x 3/8 NPT)
are supplied with the Temp-Zone package for connection to a water
circulator. The water circulator can be located underneath the work surface
of the cabinet or next to the cabinet on the floor.
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CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd
Now that the installation is properly prepared, you are ready to inspect,
install, and certify the Purifier Biosafety Cabinet. This chapter covers how to:
• Unpack and move the Biosafety Cabinet.
• Install the cabinet.
• Connect the electrical supply source.
• Connect the service lines.
• Connect to an exhaust system (optional).
• Arrange certification of the Biosafety Cabinet.
• Install the microscope (optional – Scope-Ready package only).
• Connect the circulator (optional – Temp-Zone package only).
Tools required for installation the Biosafety Cabinet include two 1/2"
wrenches, a flat-blade screwdriver, a #2 Phillips screwdriver, and a
carpenter’s level.
Note: The Biosafety Cabinet models weigh between 400–700 lbs. (182-318 kg).
The shipping pallet allows for lifting with a mechanical lift truck or floor jack. If
you must lift the product manually, use at least six (6) persons and follow safelifting guidelines.
Note: The side panels must be removed to access the fasteners that secure the
Biosafety Cabinet to the pallet. DO NOT ATTEMPT TO LIFT THE
BIOSAFETY CABINET BY THE SIDE PANELS; DAMAGE WILL
OCCUR.
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Chapter 3: Getting Started
Unpacking the Biosafety Cabinet
Carefully remove the outer carton and inspect the cabinet for damage that
may have occurred in transit. If the Biosafety Cabinet is damaged, notify the
delivery carrier immediately and retain the entire shipment intact for
inspection by the carrier.
Note: United States Interstate Commerce Commission rules require that
claims be filed with the delivery carrier within fifteen (15) days of delivery.
Do not return goods without the prior authorization of Labconco.
Unauthorized returns will not be accepted.
If the cabinet was damaged in transit, you must file a claim directly with the
freight carrier. Labconco Corporation and its dealers are not responsible for
shipping damages.
Do not discard the carton or packing material for the Biosafety Cabinet until
all of the components have been checked, installed and tested.
The cabinet is secured to the pallet in two places on each side. To access the
nuts and bolts, remove the side panels by removing and keeping the two
Phillips screws on both panels. Swing the front of each panel away from the
Cabinet, and lift it straight up to remove the panel from the cabinet.
The following are located in a box underneath the work surface:
• User’s Manual
• Drain Valve Assembly and fasteners
• Power Cord
• Product Registration Card
If you did not receive one or more of the components listed for the cabinet, or
if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.
Moving the Cabinet
Move the cabinet, attached to its pallet, by using a floor jack, or a furniture
dolly underneath the unit. DO NOT
hand truck.
8
move the cabinet by tilting it onto a
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Chapter 3: Getting Started
Preparing the Biosafety Cabinet for Operation
Installation instructions for the cabinet (Labconco P/N 1056801) are attached
to the sash of the Biosafety Cabinet. If these instructions are missing or
unclear, contact Product Service at 800-821-5525 or 816-333-8811.
Installing the Biosafety Cabinet on an Existing
Work Surface
Note: The Biosafety Cabinet is very top heavy. Use caution when lifting or
moving it.
When installing the Biosafety Cabinet onto an existing work surface or
benchtop, ensure that the structure can safely support the combined weight of
the cabinet and any related equipment. The work surface should be at least as
wide as the Cabinet and 31 inches (787 mm) deep to properly support the unit.
A hole or notch may be cut in the supporting surface in the right front corner
to accommodate the optional drain valve.
Installing the Cabinet on a Labconco Base Stand
Labconco offers accessory Base Stands in a variety of configurations to suit
the particular needs. Stands can be ordered with adjustable telescoping legs
or with a manually or electrically adjustable hydraulic lift.
Telescoping Base Stands
These stands are available with either fixed feet or casters. Adjust the leg
height before installing the cabinet on top of the stand. The height can be set
in 1-inch intervals between 27.5 to 34.5 inches (699 to 876 mm), providing a
Cabinet work surface height from 30.0 to 37.0 inches (762 to 940 mm). The
Base Stands for each width Cabinet are listed in Table 3-1 below.
Width Base Stand w/Feet Model # Base Stand w/Casters Model #
3' 3730300 3730310
4' 3730400 3730410
5' 3830500 3830510
6' 3730600 3730610
Table 3-1
1. Before positioning the Telescoping Base Stand in its final location, adjust
the height. A plastic bag containing the mounting bolts for the Biosafety
Cabinet is shipped with the base stand. Remove and save it.
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Chapter 3: Getting Started
2. Select the height of the stand and slide four (4) leg extensions into base
stand corner posts and attach with 2 ¼ inch long bolt, flatwasher,
lockwasher and nut. Ensure that the same height hole is selected for each
leg. Tighten the leg bolts securely. See Figure 3-1.
Figure 3-1
3. CAUTION: Only lift and support the weight of the cabinet at the
stainless steel sides and back. Damage will occur if lifted or supported on
the front edge. Move the base stand into its final location. Using a
carpenter’s level, adjust each leveling foot until the stand is level in both
planes as shown in Figure 3-2. You are now ready to lift the Biosafety
Cabinet onto its stand.
Figure 3-2
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Chapter 3: Getting Started
Manual or Electric Hydraulic Lift Base Stands
These base stands offer infinitely adjustable height between 25.5 and 33.5
inches (648 to 851 mm), giving a Cabinet work surface height of 28.0 to 36.0
inches. The height is adjusted either by a manual (hand crank) or electric
pump that drives hydraulic rams in the legs of the stands. All of the
hydraulic stands are equipped with fixed feet, but can be converted to caster
wheels with the addition of Caster Kit #3784000. The Base Stands for each
Cabinet model is listed in Table 3-2 below.
Width Manual Lift Stand # Electric (115V) Lift Stand # Electric (230V) Lift Stand #
3' 3780200 3780100 3780103
4' 3780201 3780101 3780104
5' 3780203 3780106 3780107
6' 3780202 3780102 3780105
Table 3-2
Note: When installing the Cabinet on the hydraulic lift base stand, ensure that
the hydraulic lines and the electrical cord are clear of any obstructions before
installing the cabinet on the stand or operating the lift system.
Connecting the Biosafety Cabinet to Utility
Service Lines
Note: Some models have a solenoid valve connected to the service valve on
the right side, rear position. The solenoid prevents gas from flowing to the
service valve when the unit blower is off. It is the only service valve position
that can be fitted with a solenoid valve. Connect the gas service to the
solenoid valve.
The service lines (if any) should be connected to the tube fitting(s) on the
outside of the liner wall as shown in Figure 3-3. To install the tubing, follow
these steps:
1. Ensure that the tubing is ¼ inch O.D., soft metal, and that the end has
been completely deburred.
2. Route the tubing from the rear of the cabinet, ensuring that it will line up
with the slot in the back of the side panel. The slot is located from 8 ¾ to
11 ¼ inches (222 to 288 mm) from the bottom of the cabinet.
Note: Make sure that the tube routing will not contact any electrical wires.
DO NOT loop service line tubing within the side panels of the Cabinet.
3. Make sure that the nut on the tube fitting is loose, but do not remove it.
Look inside the fitting to make sure the tube ferrule is there.
4. Push the tube into the fitting until it is properly seated. The tube will go
approximately ¾ inch (19 mm) into the fitting.
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11
Chapter 3: Getting Started
5. Tighten the tube fitting nut hand tight and then, using a 7/16-inch wrench,
tighten it at least ¾ turn more.
6. Close the service valve in the Biosafety Cabinet and then slowly open the
shutoff valve on the service valve. Test all fittings for leakage. Tighten
the tube nut slightly if needed.
Figure 3-3
Tube fitting nut
Tube fitting
Optional Exhaust System Connections
All Class II, Type A2 Biosafety Cabinets are shipped to recirculate their
HEPA filtered exhaust air back into the laboratory.
Certain applications such as working with odorous products or volatile toxic
materials will require the connection of the Biosafety Cabinet to an exhaust
system.
Note: The canopy connection, also referred to as a thimble or air gap
connection, allows single or multiple biohazard cabinets to be connected to
an exhaust system. During operation, the exhaust system draws all of the
cabinet’s exhaust air, plus a volume of room air (through the slots in the
canopy) into the exhaust duct. Canopy connections function as a “shock
absorber” allowing the system to function properly during changes in room
air pressure.
For information on selecting a canopy connection, go to Appendix D:
Accessories.
Warning: If the research involves the use of toxic compounds or volatile
materials, contact the facility’s safety officer or Labconco to ensure that the
Biosafety Cabinet and its exhaust system are compatible with the materials
you will be working with.
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Chapter 3: Getting Started
Drain Valve Installation
In order to prevent damage during shipping, the drain valve assembly has not
been installed. If desired, the valve should be installed after the Cabinet is in
its final location.
To install the valve assembly, follow these steps:
Note: The work surface is heavy. Use caution when handling it.
1. Lift the work surface out of the Biosafety Cabinet by lifting on the knobs
at the front of the work surface. Steady the work surface while pulling it
straight out the front of the cabinet.
2. Using a putty knife, remove and discard the stainless steel cover that is
sealed over the drain mounting holes. Scrape out remaining sealant that
is around the holes.
3. Apply a light coating of silicone sealant (user supplied) to the mounting
surface of the drain assembly. Attach the drain assembly under the
bottom of the cabinet as shown in Figure 3-4. Wipe off any excess sealant
from the cabinet bottom. Ensure that the center drain hole is
unobstructed.
4. Make sure the drain valve is in the closed position.
5. Reinstall the work surface.
6. Allow the silicone sealant to cure for at least eight hours before exposing
it to liquid.
NOTE: The drain valve
assembly attaches to the
underside of the cabinet
bottom.
Apply a light coat of silicone
sealant to this surface of the
connector, aligning the three
holes in the connector with
the three holes in the
Biosafety Cabinet liner.
Figure 3-4
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13
Chapter 3: Getting Started
Microscope Installation (optional)
In order to successfully install a user-supplied microscope in a Cell Logic
Biosafety Cabinet with Scope-Ready package, please follow the steps below
and refer to Figure 3-5 as well as Appendix F: Installation Guide for all
Pure-Vu Seals and Stand-Still Isolation Platforms:
Note: The work surface is heavy. Use caution when handling it.
1. Before installing a microscope, locate the Stand-Still Isolation
Platform that is packaged in a separate carton underneath the work
surface. Note: Stereoscopes will typically require the Stand-Still XL
Isolation Platform found in Appendix D: Accessories.
2. Install the Stand Still Isolation Platform so that the leading edge is in
line with the work surface leading edge. The Stand-Still Isolation
Platform is elevated to promote safe airflow and isolate up to 94% of
vibrations. See Appendix F for typical pictures of proper set-up of the
Stand-Still Isolation Platform.
3. With the sash in the up position, place a user-supplied microscope
inside and on top of the Stand-Still Isolation Platform. Remove the
microscope eyepieces.
4. The Cell Logic Biosafety Cabinet with Scope-Ready Package
includes one Pure-Vu Seal that requires 0.5" (12.7 mm) diameter
holes spaced approximately 2.75" (69.9 mm) apart and located from
the base of the microscope to the centerline of the eyepiece. The
eyepiece holes can be cut with the 0.5" (12.7 mm) diameter hole
Punch Kit (#4027801) supplied with your cabinet.
For your convenience, pre-cut accessory Pure-Vu Seals are available per
Appendix D: Accessories, and may be an easier installation.
5. If punching the 0.5" (12.7 mm) diameter holes, remove the
microscope eyepieces and line up the centerline of the eyepiece base
with the seal. Then mark the centerline of each eyepiece hole spaced
at approximately 2.75" (69.9 mm).
6. Remove the 14 screws (#6-32 x 0.25") that retain the Pure-Vu Seal.
7. Either re-install one of the pre-cut accessory Pure-Vu Seals from
Appendix D or punch the holes in the blank seal supplied and then reinstall the seal. For your convenience, see Appendix F for installation
of pleated Pure-Vu XL Seals used with stereoscopes requiring
frequent eyepiece height adjustment of +/- 2.0" (51 mm).
8. After the seal is re-installed, then move the microscope forward to
carefully stretch the Pure-Vu Seal over both of the microscope
eyepiece bases.
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Chapter 3: Getting Started
9. Finally, re-install the microscope eyepieces.
10. The Pure-Vu Seal should now provide an optimum seal for safe
airflow and ergonomic visibility.
Outer
Aerodynamic
Collar
Pure-Vu Seal
Mounting
Screws
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Figure 3-5
15
Chapter 3: Getting Started
Circulator Installation (optional)
In order to successfully install a user-supplied circulator on a Cell Logic
Biosafety Cabinet with Temp-Zone package please follow the steps below
and refer to Figure 3-6:
Note: The work surface is heavy and two Easy-Clean™ Prop Rods are
provided to pivot and support the Temp-Zone work area and the work
surface.
1. The Temp-Zone work area has supply and return lines for the usersupplied circulator. Refer to Figure 3-6 and locate the two quick
connect elbows on the left side of the cabinet behind the left side
panel.
2. Find a suitable place next to the cabinet or underneath the cabinet to
place the circulator. Accessory shelves for small circulators are
available from Appendix D: Accessories. Larger circulators should be
placed on the floor.
3. Pivot the work surface up and use the Easy-Clean Prop Rods to
support the work surface. Then locate the tubing, insulation, and
quick connect fittings shipped with your Cell Logic. The tubing is
3/8" OD (9.5 mm) and tube inserts must be placed inside the tubing to
properly support the tubing inside the quick connect fitting. 20 feet
(6.1 meters) of tubing length is provided for your convenience.
Note: The tube inserts prevent the tubing from collapsing at
connection points and must be used.
4. Plan, route and cut the tubing from the two elbows on the left side of
the cabinet to the circulator.
5. Locate the quick connect fittings (3/8" tube x 1/8 NPT, 3/8" tube x 1/4
NPT, and 3/8" tube x 3/8 NPT) and install the appropriate fittings to the
user-supplied circulator with appropriate pipe sealant or Teflon tape.
6. Place tube inserts in all ends of the cut-to-size tubing.
7. Cut and install the insulation around the cut tubing lengths.
8. Insert cut tubing lengths into the elbows on the cabinet and then
install both tubing lengths into the circulator to complete the water
circulator loop.
9. Read and follow the circulator instructions before operating. The
circulator should have a minimum flow rate of 0.5 GPM (1.9 LPM).
In all cases, the temperature of the circulator will not equal the
temperature of samples on the Temp-Zone. Factors such as the
sample vessels, sample sizes, aluminum tube racks, and length of
tubing connecting the circulator to the Temp-Zone can influence the
sample temperature. Circulator settings to hold heated incubator
sample temperatures may require 0.3-2.0°C higher temperatures than
the desired sample temperature. Furthermore, circulator settings to
maintain chilled refrigerated samples may require 0.3-3.0°C colder
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Chapter 3: Getting Started
temperatures than the desired sample temperature. Experimentation is
necessary to achieve proper sample temperatures.
10. For chilled applications, the Temp-Zone work area comes with a drain
line installed that can be routed to the cabinet’s drain valve, if desired.
This Temp-Zone drain line installation is optional for chilled
applications should excessive condensation occur under the work
surface. Simply snap in the supplied press-fit elbow to the drain valve
shown in Figure 3-4. Follow the diagram from Figure 3-6 to finish
the installation.
Quick-Connect
Elbow Fitting
Circulator
(User-Supplied)
Product Service 1-800-522-7658
Circulator Tubing
and Insulation
Figure 3-6
Prop Rod
Press-fit
Elbow
Drain Valve
Optional
Drain Line
Temp-Zone
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Chapter 3: Getting Started
Initial Certification
Prior to use, a qualified certifier should certify all Biosafety Cabinets. Under
normal operating conditions, the Biosafety Cabinet should be recertified at
least annually and when relocated or serviced. The certifier should perform
the following tests, as recommended in ANSI/NSF International Standard
Number 49 in effect when the cabinet was manufactured:
• Downflow Velocity Profile Test
• Inflow Velocity Test
• Airflow Smoke Patterns
• HEPA Filter Leak Test
• Vibration Test *
• Noise Level Test *
• Lighting Intensity Test *
*These tests are user comfort related tests and may be omitted at the
user’s or certifier’s discretion.
If you have any questions regarding certification agencies or need assistance
in locating one, contact Labconco’s Product Service Department at 1-800522-7658 or 816-333-8811.
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Product Service 1-800-522-7658
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All Purifier Series Cell Logic Biosafety Cabinets operate using the following
principles:
• Filtration and retention of particulates by High Efficiency Particulate
Air (HEPA) filter(s)
• Laminar airflow
• Directional airflow
The major components in a Biosafety Cabinet are:
• The HEPA filter(s) or optional ULPA filters
• The motor/blower to force air through the Cabinet
• Cabinet air intakes (grilles), ductwork and air balance controls
The two major package options of a Cell Logic Biosafety Cabinet are:
• The Scope-Ready™ package option to allow a user-supplied
microscope to be integrated into the cabinet.
• The Temp-Zone™ package option that includes a temperature
controlled work area when connected to a user-supplied circulator.
HEPA Filters
HEPA filters are disposable, dry-type particulate filters. The filter material or
media is typically made of borosilicate microfibers formed into a thin sheet,
in a process similar to the production of paper. This sheet is folded, or pleated
to increase its surface area. The pleats are held in place by aluminum
diffusers or by beads of glue that add rigidity to the media pack. The pack is
then set into a frame, and sealed as shown in Figure 4-1.
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Chapter 4: Performance Features and Safety Precautions
The HEPA filter manufacturer establishes the efficiency of the filter by
challenging it with an aerosol of known particle size. The number of particles
that penetrate the filter are quantified, and this establishes the efficiency of
the filter. Thus, the filters used in the Purifier Cell logic Biosafety Cabinets
are at least 99.99% efficient in removing particles 0.3 micron.
Note: The HEPA filter media is very fragile. DO NOT touch the media. If
you think the media of a HEPA filter is damaged, DO NOT USE THE
CABINET. Have the HEPA filter integrity tested by a certifier before using
the cabinet.
Note: HEPA Filters are only effective against particulate material. Gases
will pass through the filter.
Polyurethane seal
Between media pack
and filter frame
Figure 4-1
Filter frame
Filter media pack –
A continuous sheet
of filter media
ULPA Filters
Optional ULPA filters may be used to replace the standard HEPA filters in
the Purifier Cell Logic Biosafety Cabinets. ULPA filters have the same
properties as described above except they are rated at least 99.999% efficient
in removing particles 0.1-0.2 or 0.2-0.3 micron.
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Chapter 4: Performance Features and Safety Precautions
Laminar Airflow
Laminar airflow is defined as the movement of a body of air in a single
direction, with a uniform velocity. In practice, the laminar downflow of air in
the cabinet captures any aerosol generated in the work area of the cabinet,
and directs it to the HEPA filters. In order to be true laminar downflow, a
number of individual downflow velocity test points (The Downflow Velocity
Profile) must be +/- 16 feet per minute (0.08 m/s) of the average of all the test
points. This is illustrated in Figure 4-2.
Figure 4-2
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