Labconco Biological Safety Cabinets 34609 User Manual

User’s Manual

Purifier®CELL Series
Biological Safety Cabinets

Models

34309 Series 34509 Series
34409 Series 34609 Series

TYPE

A2

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Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX [phone]
E-MAIL [e-mail]
HOME PAGE www.labconco.com

Please read the User’s Manual before operating the equipment.

Copyright © 2009 Labconco Corporation. All rights reserved.

The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.

Warranty

Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.

The warranty for all Labconco products will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner, except the following;

• Purifier® Logic® and Cell Logic® Biological Safety Cabinets and PuriCare® Lab Animal
Research Stations carry a three-year warranty from date of installation or four years from date
of shipment from Labconco, whichever is sooner.

• SteamScrubber® & FlaskScrubber® Glassware Washers carry a two-year warranty from date
of installation or three years from date of shipment from Labconco, whichever is sooner.

• Blood Drawing Chairs carry a ten year warranty.

• Carts carry a lifetime warranty.

• Glassware is not warranted from breakage when dropped or mishandled.

This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.

Returned or Damaged Goods

Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight
carrier. Labconco Corporation and its dealers are not responsible for shipping damages.

The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.

Limitation of Liability

The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar
with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon
water, land, or air and to comply with such regulations. Labconco Corporation is held harmless with
respect to user’s compliance with such regulations.

Contacting Labconco Corporation

If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821­5525 or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.

Part #4028900, Rev. B

ECO F719

T

AABBLLEE

T

CHAPTER 1: INTRODUCTION 1

CHAPTER 2: PREREQUISITES 2

Space Requirements 2
Clearance 2
Location Requirements 3
Exhaust Requirements 4
Electrical Requirements 4
Service Line Requirements 5
User-Supplied Microscope Requirements 5
User-Supplied Circulator Requirements 6

CHAPTER 3: GETTING STARTED 7
Unpacking the Biosafety Cabinet 8
Moving the Cabinet 8
Preparing the Biosafety Cabinet for Operation 9
Installing the Biosafety Cabinet on an Existing Work Surface 9
Installing the Cabinet on a Labconco Base Stand 9
Telescoping Base Stands 9
Manual or Electric Hydraulic Lift Base Stands 11
Connecting the Biosafety Cabinet to Utility Service Lines 11
Optional Exhaust System Connections 12
Drain Valve Installation 13
Microscope Installation (optional) 14
Circulator Installation (optional) 16
Initial Certification 18

CHAPTER 4: PERFORMANCE FEATURES AND SAFETY

HEPA Filters 19
ULPA Filters 20
Laminar Airflow 21
Directional Airflow 22
Motor/Blower 23
Cabinet Air Intakes (Grilles), Ductwork & Air Balance Controls 24

O

O

PRECAUTIONS 19

FF

C

C

OONNTTEENNTTSS

Ultraviolet (UV) Lamp 24
Scope-Ready Package Option 25
Temp-Zone Package Option 25
Safety Precautions 26

CHAPTER 5: USING THE CABINET 28
System Reset Switch 28

Information Center 29
Status Area 29
Data Area 30
Icon Area 30
Operating the Sliding Sash 31
Starting the Biosafety Cabinet 32
The Logic Touchpad 33

User Selectable Features 38
Timer Operation 38
Interval Timer Operation 39
Stopwatch Timer Operation 39
Menu Options 39
UV Lamp Operation 39
UV Lamp Timer 40
UV Lamp Maintenance 40
Light Operation 41
Light Timer 41
Light Maintenance 41
Blower Operation 42
Cabinet Set Up 42
If An Airflow Alert Activates 45
Resetting the Airflow Alert System 45
Working in the Biosafety Cabinet 45

CHAPTER 6: MAINTAINING THE CABINET 48
Routine Maintenance Schedule 48
Service Operations 49
Work Surface Removal 49
Front Grille Removal 49
Towel Catch Removal 50
Front Panel Removal & Installation 51
Changing the Fluorescent Lamps 52
Changing the Optional UV Lamp 52
Resetting a Circuit Breaker 53
Storage 53

CHAPTER 7: TROUBLESHOOTING 54

APPENDIX A: COMPONENTS 57

APPENDIX B: DIMENSIONS 59

APPENDIX C: SPECIFICATIONS 61

Electrical Data 61
Motor Specifications 61
Environmental Conditions 62

APPENDIX D: ACCESSORIES 63
Telescoping Base Stands 63
Telescoping Base Stand Shelf Kit 63
Manual or Electric Hydraulic Lift Base Stands 63
Hydraulic Base Stand Shelf Kit 64
SoLo Electric Hydraulic Lift Base Stands 64
Seismic Bracket Kit 64
Service Valve Kit 64
Ultraviolet Lamp Kits 64
IV Bar Kits 65
Shelf 65
Cord/Tubing Management System 65
Prefilter 65
Postfilter 65
Postfilter Conversion Kit 66
Canopy Connection Kit 66
Air-Tight Damper 66
Backdraft Damper 66
Remote Blowers 66
Ergonomic Chair with Armrests 67
Adjustable Footrest 67
Inflow/Downflow Sensor Kit 67
Aspirator Pump Kit 67
Turntable 67
ULPA Supply Filters 67
ULPA Exhaust Filters 67
Pure-Vu Air Barrier Seals 68
Stand-Still Isolation Platforms 69

Tube Racks/Blocks/Holders, Circulator Shelf,
Connectors and Tubing 69

APPENDIX E: QUICK CHART 70

APPENDIX F: INSTALLATION GUIDE FOR ALL PURE-VU
SEALS AND STAND-STILL ISOLATION
PLATFORMS 71

DECLARATION OF CONFORMITY 80

CChhaapptteerr 11::
IInnttrroodduuccttiioonn

Congratulations on the purchase of a Labconco Purifier® Cell Logic
Biosafety Cabinet. The Biosafety Cabinet is designed to protect you, the
product and the laboratory environment from biohazardous aerosols. The
Cell Logic Series Cabinet is the result of users like you requiring a customer
supplied microscope integrated into the cabinet and a temperature controlled
work area to maintain sample temperature. The Cell Logic Biosafety Cabinet
is available with either or both Scope-Ready™ and Temp-Zone™ package
options.

The Biosafety Cabinet offers many unique features to enhance safety,
performance and ergonomics. To take full advantage of them, please
acquaint yourself with this manual and keep it handy for future reference. If
you are unfamiliar with how biosafety cabinets operate, please review
Chapter 4: Performance Features and Safety Precautions before you begin
working in the cabinet. Even if you are an experienced biosafety cabinet
user, please review Chapter 5: Using the Cabinet; it describes the Biosafety
Cabinet’s features so that you can use it efficiently.

This manual and other technical information is available in PDF format
at our website: www.labconco.com.

®

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CChhaapptteerr 22::
PPrreerreeqquuiissiitteess

Before you install the Biosafety Cabinet, you need to prepare the site for
installation. Examine the location where you intend to install the cabinet.
You must be certain that the area is level and of solid construction. In
addition, a dedicated source of electrical power must be located near the
installation site.

Carefully read this chapter to learn:

• Location requirements.

• Electrical power requirements.

• Exhaust requirements.

• Service utility requirements.

• Space requirements.

• User-supplied microscope requirements (if needed)

• User-supplied circulator requirements (if needed)

Refer to Appendix C: Specifications, for complete biosafety cabinet electrical
and environmental conditions, specifications and requirements.

Space Requirements

The dimensions for the Cell Logic A2 Biosafety Cabinets are shown in Appendix
B: Dimensions.

Clearance

A minimum clearance of at least 6 inches (150 mm) is suggested on the top
and both sides of the cabinet for service.

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Chapter 2: Prerequisites

Location Requirements

Note: The Biosafety Cabinet should be located away from traffic patterns,
doors, fans, ventilation registers, fume hoods and any other air-handling
devices that could disrupt its airflow patterns. All windows in the room
should remain closed. Figure 2-1 shows the preferred location for the
Biosafety Cabinet.

Preferred location

Air register blocked or redirected

to prevent cabinet disruption

Figure 2-1

Alternate location

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Chapter 2: Prerequisites

Exhaust Requirements

If you intend to connect the Biosafety Cabinet to the optional Canopy
Connection Kit, first examine the location to ensure that it accommodates the
cabinet’s exhaust duct. The area directly above the cabinet’s exhaust port
should be clear of structural elements, water and utility lines, or other fixed
obstructions. There should be enough clearance to accommodate a 10-inch
diameter duct. Avoid cabinet locations that require an elbow directly above
the cabinet’s exhaust connection or an excessive number of elbows in the
exhaust system. For further information about the cabinet’s exhaust system
requirements, please refer to Chapter 3: Getting Started.

Electrical Requirements

The different Biosafety Cabinet models have the following electrical
requirements:

Voltage

and Size Base Model Last Model Digits Requirements

115V

3'
4'
5'
6'

100V

3'
4'
5'
6'

230V

3'
4'
5'
6'

34309-xx
34409-xx
34509-xx
34609-xx

34309-xx
34409-xx
34509-xx
34609-xx

34309-xx
34409-xx
34509-xx
34609-xx

115 VAC, 60 Hz, 12 Amps

-30 to 31, -60 to 61, -80 to 81
115 VAC, 60 Hz, 16 Amps

100 VAC, 50/60 Hz, 12 Amps

-32 to 33, -62 to 63, -82 to 83

100 VAC, 50/60 Hz, 16 Amps

230 VAC, 50/60 Hz, 6 Amps

-34 to 43, -64 to 73, -84 to 93

230 VAC, 50/60 Hz, 8 Amps

Table 2-1

Note: A dedicated outlet with a circuit breaker rated at 20 amps should
be located as close as possible to the right rear side of the cabinet, at a
height even with, or higher than, the top of the bench or stand.

Note: On 115 VAC models, both electrical outlets are protected by a ground

fault interrupter circuit (GFIC). Labconco does not recommend plugging the
Biosafety Cabinet into a GFIC outlet.

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Chapter 2: Prerequisites

Service Line Requirements

All utility service lines should be ¼ inch O.D., brass, copper, or stainless
steel, and equipped with an easily accessible shut-off valve. If the service
line pressure exceeds 100 PSI, it must be equipped with a pressure regulator
to reduce the line pressure.

Note: The use of flammable gases or solvents should be avoided in the
Biosafety Cabinet. Open flame in the cabinet will disrupt the laminar airflow
in the cabinet and may damage the HEPA filters. Flammable gases or
solvents may reach explosive concentrations in the cabinet or ductwork. If
you feel that the procedure requires the use of an open flame or flammable
materials, contact your institution’s safety office.

The use of air or gases under high pressure should be avoided as they may
seriously disrupt the airflow patterns in the cabinet.

User-Supplied Microscope Requirements –
Purifier Cell Logic Biosafety Cabinets with
Scope-Ready™ Package Only

If installing a microscope, locate the Stand-Still™ Isolation Platform in a
separate box shipped with the cabinet. The Stand-Still Isolation Platform
supports the microscope on top of the stainless steel work surface to
eliminate up to 94% of vibrations and to promote safe airflow. If using a
stereoscope with a forward extending base, the extended Stand-Still XL
Isolation Platform should be ordered from Appendix D: Accessories. Figures
from Appendix F should be used to assist your set-up.

On models with the Scope-Ready package, the Cell Logic Biosafety Cabinet
includes a clear, disposable Pure-Vu™ Seal that can be altered to
accommodate most microscope’s eyepieces. Utilize the Punch Kit
(#4027801), supplied with your cabinet, to cut the two eyepiece holes at
approximately 2.75" (69.9 mm) spacing to match the height of your scope.
As a convenience, pre-cut Pure-Vu eyepiece seals can be ordered from
Appendix D: Accessories. The pre-cut accessory Pure-Vu Seals are based off
the nominal height from the base of the microscope to eyepiece centerline
and can save you valuable time modifying the seal shipped with the Cell
Logic. If using a stereoscope with a forward extending base, one of the Pure­Vu XL Seals should be ordered from Appendix D: Accessories. Figures from
Appendix F should be used to assist your set-up for all Pure-Vu Seals.

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5

Chapter 2: Prerequisites

User Supplied Circulator Requirements – Purifier
Cell Logic Biosafety Cabinets with Temp Zone™
Package Only

The Temp Zone™ features a temperature controlled area on the work surface
where heated or chilled samples may be transferred and kept at optimum
temperature. A user-supplied circulator that continually delivers heated or
chilled water at a minimum flow rate of 0.5 GPM (1.9 LPM) with typical
volume of 6 liters is required and may be obtained from your laboratory
supply dealer. See Figure 3-6 in Chapter 3.

Quick connection fittings are included on the left side of the cabinet and are
sized for 3/8" diameter polyethylene tubing. As a convenience, an ample
supply (20'; 6 m) of 3/8" (9.5 mm) diameter tubing, insulation, and quick
connect fittings (3/8 tube x 1/8 NPT, 3/8 tube x 1/4 NPT, 3/8 tube x 3/8 NPT)
are supplied with the Temp-Zone package for connection to a water
circulator. The water circulator can be located underneath the work surface
of the cabinet or next to the cabinet on the floor.

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CChhaapptteerr 33::
GGeettttiinngg SSttaarrtteedd

Now that the installation is properly prepared, you are ready to inspect,
install, and certify the Purifier Biosafety Cabinet. This chapter covers how to:

• Unpack and move the Biosafety Cabinet.

• Install the cabinet.

• Connect the electrical supply source.

• Connect the service lines.

• Connect to an exhaust system (optional).

• Arrange certification of the Biosafety Cabinet.

• Install the microscope (optional – Scope-Ready package only).

• Connect the circulator (optional – Temp-Zone package only).

Tools required for installation the Biosafety Cabinet include two 1/2"
wrenches, a flat-blade screwdriver, a #2 Phillips screwdriver, and a
carpenter’s level.

Note: The Biosafety Cabinet models weigh between 400–700 lbs. (182-318 kg).
The shipping pallet allows for lifting with a mechanical lift truck or floor jack. If
you must lift the product manually, use at least six (6) persons and follow safe­lifting guidelines.

Note: The side panels must be removed to access the fasteners that secure the
Biosafety Cabinet to the pallet. DO NOT ATTEMPT TO LIFT THE

BIOSAFETY CABINET BY THE SIDE PANELS; DAMAGE WILL
OCCUR.

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Chapter 3: Getting Started

Unpacking the Biosafety Cabinet

Carefully remove the outer carton and inspect the cabinet for damage that
may have occurred in transit. If the Biosafety Cabinet is damaged, notify the
delivery carrier immediately and retain the entire shipment intact for
inspection by the carrier.

Note: United States Interstate Commerce Commission rules require that
claims be filed with the delivery carrier within fifteen (15) days of delivery.

Do not return goods without the prior authorization of Labconco.
Unauthorized returns will not be accepted.

If the cabinet was damaged in transit, you must file a claim directly with the
freight carrier. Labconco Corporation and its dealers are not responsible for
shipping damages.

Do not discard the carton or packing material for the Biosafety Cabinet until
all of the components have been checked, installed and tested.

The cabinet is secured to the pallet in two places on each side. To access the
nuts and bolts, remove the side panels by removing and keeping the two
Phillips screws on both panels. Swing the front of each panel away from the
Cabinet, and lift it straight up to remove the panel from the cabinet.

The following are located in a box underneath the work surface:

• User’s Manual

• Drain Valve Assembly and fasteners

• Power Cord

• Product Registration Card

If you did not receive one or more of the components listed for the cabinet, or
if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.

Moving the Cabinet

Move the cabinet, attached to its pallet, by using a floor jack, or a furniture
dolly underneath the unit. DO NOT
hand truck.

8

move the cabinet by tilting it onto a

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Chapter 3: Getting Started

Preparing the Biosafety Cabinet for Operation

Installation instructions for the cabinet (Labconco P/N 1056801) are attached
to the sash of the Biosafety Cabinet. If these instructions are missing or
unclear, contact Product Service at 800-821-5525 or 816-333-8811.

Installing the Biosafety Cabinet on an Existing
Work Surface

Note: The Biosafety Cabinet is very top heavy. Use caution when lifting or
moving it.

When installing the Biosafety Cabinet onto an existing work surface or
benchtop, ensure that the structure can safely support the combined weight of
the cabinet and any related equipment. The work surface should be at least as
wide as the Cabinet and 31 inches (787 mm) deep to properly support the unit.

A hole or notch may be cut in the supporting surface in the right front corner
to accommodate the optional drain valve.

Installing the Cabinet on a Labconco Base Stand

Labconco offers accessory Base Stands in a variety of configurations to suit
the particular needs. Stands can be ordered with adjustable telescoping legs
or with a manually or electrically adjustable hydraulic lift.

Telescoping Base Stands

These stands are available with either fixed feet or casters. Adjust the leg
height before installing the cabinet on top of the stand. The height can be set
in 1-inch intervals between 27.5 to 34.5 inches (699 to 876 mm), providing a
Cabinet work surface height from 30.0 to 37.0 inches (762 to 940 mm). The
Base Stands for each width Cabinet are listed in Table 3-1 below.

Width Base Stand w/Feet Model # Base Stand w/Casters Model #

3' 3730300 3730310

4' 3730400 3730410

5' 3830500 3830510

6' 3730600 3730610

Table 3-1

1. Before positioning the Telescoping Base Stand in its final location, adjust
the height. A plastic bag containing the mounting bolts for the Biosafety
Cabinet is shipped with the base stand. Remove and save it.

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Chapter 3: Getting Started

2. Select the height of the stand and slide four (4) leg extensions into base
stand corner posts and attach with 2 ¼ inch long bolt, flatwasher,
lockwasher and nut. Ensure that the same height hole is selected for each
leg. Tighten the leg bolts securely. See Figure 3-1.

Figure 3-1

3. CAUTION: Only lift and support the weight of the cabinet at the
stainless steel sides and back. Damage will occur if lifted or supported on
the front edge. Move the base stand into its final location. Using a
carpenter’s level, adjust each leveling foot until the stand is level in both
planes as shown in Figure 3-2. You are now ready to lift the Biosafety
Cabinet onto its stand.

Figure 3-2

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Chapter 3: Getting Started

Manual or Electric Hydraulic Lift Base Stands

These base stands offer infinitely adjustable height between 25.5 and 33.5
inches (648 to 851 mm), giving a Cabinet work surface height of 28.0 to 36.0
inches. The height is adjusted either by a manual (hand crank) or electric
pump that drives hydraulic rams in the legs of the stands. All of the
hydraulic stands are equipped with fixed feet, but can be converted to caster
wheels with the addition of Caster Kit #3784000. The Base Stands for each
Cabinet model is listed in Table 3-2 below.

Width Manual Lift Stand # Electric (115V) Lift Stand # Electric (230V) Lift Stand #

3' 3780200 3780100 3780103

4' 3780201 3780101 3780104

5' 3780203 3780106 3780107

6' 3780202 3780102 3780105

Table 3-2

Note: When installing the Cabinet on the hydraulic lift base stand, ensure that
the hydraulic lines and the electrical cord are clear of any obstructions before
installing the cabinet on the stand or operating the lift system.

Connecting the Biosafety Cabinet to Utility
Service Lines

Note: Some models have a solenoid valve connected to the service valve on
the right side, rear position. The solenoid prevents gas from flowing to the
service valve when the unit blower is off. It is the only service valve position
that can be fitted with a solenoid valve. Connect the gas service to the
solenoid valve.

The service lines (if any) should be connected to the tube fitting(s) on the
outside of the liner wall as shown in Figure 3-3. To install the tubing, follow
these steps:

1. Ensure that the tubing is ¼ inch O.D., soft metal, and that the end has
been completely deburred.

2. Route the tubing from the rear of the cabinet, ensuring that it will line up
with the slot in the back of the side panel. The slot is located from 8 ¾ to
11 ¼ inches (222 to 288 mm) from the bottom of the cabinet.

Note: Make sure that the tube routing will not contact any electrical wires.
DO NOT loop service line tubing within the side panels of the Cabinet.

3. Make sure that the nut on the tube fitting is loose, but do not remove it.
Look inside the fitting to make sure the tube ferrule is there.

4. Push the tube into the fitting until it is properly seated. The tube will go
approximately ¾ inch (19 mm) into the fitting.

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Chapter 3: Getting Started

5. Tighten the tube fitting nut hand tight and then, using a 7/16-inch wrench,
tighten it at least ¾ turn more.

6. Close the service valve in the Biosafety Cabinet and then slowly open the
shutoff valve on the service valve. Test all fittings for leakage. Tighten
the tube nut slightly if needed.

Figure 3-3

Tube fitting nut

Tube fitting

Optional Exhaust System Connections

All Class II, Type A2 Biosafety Cabinets are shipped to recirculate their
HEPA filtered exhaust air back into the laboratory.

Certain applications such as working with odorous products or volatile toxic
materials will require the connection of the Biosafety Cabinet to an exhaust
system.

Note: The canopy connection, also referred to as a thimble or air gap
connection, allows single or multiple biohazard cabinets to be connected to
an exhaust system. During operation, the exhaust system draws all of the
cabinet’s exhaust air, plus a volume of room air (through the slots in the
canopy) into the exhaust duct. Canopy connections function as a “shock
absorber” allowing the system to function properly during changes in room
air pressure.

For information on selecting a canopy connection, go to Appendix D:

Accessories.

Warning: If the research involves the use of toxic compounds or volatile
materials, contact the facility’s safety officer or Labconco to ensure that the
Biosafety Cabinet and its exhaust system are compatible with the materials
you will be working with.

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Chapter 3: Getting Started

Drain Valve Installation

In order to prevent damage during shipping, the drain valve assembly has not
been installed. If desired, the valve should be installed after the Cabinet is in
its final location.

To install the valve assembly, follow these steps:

Note: The work surface is heavy. Use caution when handling it.

1. Lift the work surface out of the Biosafety Cabinet by lifting on the knobs
at the front of the work surface. Steady the work surface while pulling it
straight out the front of the cabinet.

2. Using a putty knife, remove and discard the stainless steel cover that is
sealed over the drain mounting holes. Scrape out remaining sealant that
is around the holes.

3. Apply a light coating of silicone sealant (user supplied) to the mounting
surface of the drain assembly. Attach the drain assembly under the
bottom of the cabinet as shown in Figure 3-4. Wipe off any excess sealant
from the cabinet bottom. Ensure that the center drain hole is
unobstructed.

4. Make sure the drain valve is in the closed position.

5. Reinstall the work surface.

6. Allow the silicone sealant to cure for at least eight hours before exposing
it to liquid.

NOTE: The drain valve
assembly attaches to the
underside of the cabinet
bottom.

Apply a light coat of silicone
sealant to this surface of the
connector, aligning the three
holes in the connector with
the three holes in the
Biosafety Cabinet liner.

Figure 3-4

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13

Chapter 3: Getting Started

Microscope Installation (optional)

In order to successfully install a user-supplied microscope in a Cell Logic
Biosafety Cabinet with Scope-Ready package, please follow the steps below
and refer to Figure 3-5 as well as Appendix F: Installation Guide for all
Pure-Vu Seals and Stand-Still Isolation Platforms:

Note: The work surface is heavy. Use caution when handling it.

1. Before installing a microscope, locate the Stand-Still Isolation
Platform that is packaged in a separate carton underneath the work
surface. Note: Stereoscopes will typically require the Stand-Still XL
Isolation Platform found in Appendix D: Accessories.

2. Install the Stand Still Isolation Platform so that the leading edge is in
line with the work surface leading edge. The Stand-Still Isolation
Platform is elevated to promote safe airflow and isolate up to 94% of
vibrations. See Appendix F for typical pictures of proper set-up of the
Stand-Still Isolation Platform.

3. With the sash in the up position, place a user-supplied microscope
inside and on top of the Stand-Still Isolation Platform. Remove the
microscope eyepieces.

4. The Cell Logic Biosafety Cabinet with Scope-Ready Package
includes one Pure-Vu Seal that requires 0.5" (12.7 mm) diameter
holes spaced approximately 2.75" (69.9 mm) apart and located from
the base of the microscope to the centerline of the eyepiece. The
eyepiece holes can be cut with the 0.5" (12.7 mm) diameter hole
Punch Kit (#4027801) supplied with your cabinet.

For your convenience, pre-cut accessory Pure-Vu Seals are available per
Appendix D: Accessories, and may be an easier installation.

5. If punching the 0.5" (12.7 mm) diameter holes, remove the
microscope eyepieces and line up the centerline of the eyepiece base
with the seal. Then mark the centerline of each eyepiece hole spaced
at approximately 2.75" (69.9 mm).

6. Remove the 14 screws (#6-32 x 0.25") that retain the Pure-Vu Seal.

7. Either re-install one of the pre-cut accessory Pure-Vu Seals from
Appendix D or punch the holes in the blank seal supplied and then re­install the seal. For your convenience, see Appendix F for installation
of pleated Pure-Vu XL Seals used with stereoscopes requiring
frequent eyepiece height adjustment of +/- 2.0" (51 mm).

8. After the seal is re-installed, then move the microscope forward to
carefully stretch the Pure-Vu Seal over both of the microscope
eyepiece bases.

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Chapter 3: Getting Started

9. Finally, re-install the microscope eyepieces.

10. The Pure-Vu Seal should now provide an optimum seal for safe
airflow and ergonomic visibility.

Outer
Aerodynamic
Collar

Pure-Vu Seal

Mounting
Screws

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Figure 3-5

15

Chapter 3: Getting Started

Circulator Installation (optional)

In order to successfully install a user-supplied circulator on a Cell Logic
Biosafety Cabinet with Temp-Zone package please follow the steps below
and refer to Figure 3-6:

Note: The work surface is heavy and two Easy-Clean™ Prop Rods are
provided to pivot and support the Temp-Zone work area and the work
surface.

1. The Temp-Zone work area has supply and return lines for the user­supplied circulator. Refer to Figure 3-6 and locate the two quick
connect elbows on the left side of the cabinet behind the left side
panel.

2. Find a suitable place next to the cabinet or underneath the cabinet to
place the circulator. Accessory shelves for small circulators are
available from Appendix D: Accessories. Larger circulators should be
placed on the floor.

3. Pivot the work surface up and use the Easy-Clean Prop Rods to
support the work surface. Then locate the tubing, insulation, and
quick connect fittings shipped with your Cell Logic. The tubing is
3/8" OD (9.5 mm) and tube inserts must be placed inside the tubing to
properly support the tubing inside the quick connect fitting. 20 feet
(6.1 meters) of tubing length is provided for your convenience.
Note: The tube inserts prevent the tubing from collapsing at
connection points and must be used.

4. Plan, route and cut the tubing from the two elbows on the left side of
the cabinet to the circulator.

5. Locate the quick connect fittings (3/8" tube x 1/8 NPT, 3/8" tube x 1/4
NPT, and 3/8" tube x 3/8 NPT) and install the appropriate fittings to the
user-supplied circulator with appropriate pipe sealant or Teflon tape.

6. Place tube inserts in all ends of the cut-to-size tubing.

7. Cut and install the insulation around the cut tubing lengths.

8. Insert cut tubing lengths into the elbows on the cabinet and then
install both tubing lengths into the circulator to complete the water
circulator loop.

9. Read and follow the circulator instructions before operating. The
circulator should have a minimum flow rate of 0.5 GPM (1.9 LPM).
In all cases, the temperature of the circulator will not equal the
temperature of samples on the Temp-Zone. Factors such as the
sample vessels, sample sizes, aluminum tube racks, and length of
tubing connecting the circulator to the Temp-Zone can influence the
sample temperature. Circulator settings to hold heated incubator
sample temperatures may require 0.3-2.0°C higher temperatures than
the desired sample temperature. Furthermore, circulator settings to
maintain chilled refrigerated samples may require 0.3-3.0°C colder

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temperatures than the desired sample temperature. Experimentation is
necessary to achieve proper sample temperatures.

10. For chilled applications, the Temp-Zone work area comes with a drain
line installed that can be routed to the cabinet’s drain valve, if desired.
This Temp-Zone drain line installation is optional for chilled
applications should excessive condensation occur under the work
surface. Simply snap in the supplied press-fit elbow to the drain valve
shown in Figure 3-4. Follow the diagram from Figure 3-6 to finish
the installation.

Quick-Connect
Elbow Fitting

Circulator
(User-Supplied)

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Circulator Tubing
and Insulation

Figure 3-6

Prop Rod

Press-fit
Elbow

Drain Valve

Optional
Drain Line

Temp-Zone

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Initial Certification

Prior to use, a qualified certifier should certify all Biosafety Cabinets. Under
normal operating conditions, the Biosafety Cabinet should be recertified at
least annually and when relocated or serviced. The certifier should perform
the following tests, as recommended in ANSI/NSF International Standard
Number 49 in effect when the cabinet was manufactured:

• Downflow Velocity Profile Test

• Inflow Velocity Test

• Airflow Smoke Patterns

• HEPA Filter Leak Test

• Vibration Test *

• Noise Level Test *

• Lighting Intensity Test *

*These tests are user comfort related tests and may be omitted at the

user’s or certifier’s discretion.

If you have any questions regarding certification agencies or need assistance
in locating one, contact Labconco’s Product Service Department at 1-800­522-7658 or 816-333-8811.

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maannccee FFeeaattuurreess aanndd

SSaaffeettyy PPrreeccaauuttiioonnss

All Purifier Series Cell Logic Biosafety Cabinets operate using the following
principles:

• Filtration and retention of particulates by High Efficiency Particulate
Air (HEPA) filter(s)

• Laminar airflow

• Directional airflow

The major components in a Biosafety Cabinet are:

• The HEPA filter(s) or optional ULPA filters

• The motor/blower to force air through the Cabinet

• Cabinet air intakes (grilles), ductwork and air balance controls

The two major package options of a Cell Logic Biosafety Cabinet are:

• The Scope-Ready™ package option to allow a user-supplied
microscope to be integrated into the cabinet.

• The Temp-Zone™ package option that includes a temperature
controlled work area when connected to a user-supplied circulator.

HEPA Filters

HEPA filters are disposable, dry-type particulate filters. The filter material or
media is typically made of borosilicate microfibers formed into a thin sheet,
in a process similar to the production of paper. This sheet is folded, or pleated
to increase its surface area. The pleats are held in place by aluminum
diffusers or by beads of glue that add rigidity to the media pack. The pack is
then set into a frame, and sealed as shown in Figure 4-1.

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The HEPA filter manufacturer establishes the efficiency of the filter by
challenging it with an aerosol of known particle size. The number of particles
that penetrate the filter are quantified, and this establishes the efficiency of
the filter. Thus, the filters used in the Purifier Cell logic Biosafety Cabinets
are at least 99.99% efficient in removing particles 0.3 micron.

Note: The HEPA filter media is very fragile. DO NOT touch the media. If
you think the media of a HEPA filter is damaged, DO NOT USE THE
CABINET. Have the HEPA filter integrity tested by a certifier before using
the cabinet.

Note: HEPA Filters are only effective against particulate material. Gases
will pass through the filter.

Polyurethane seal
Between media pack
and filter frame

Figure 4-1

Filter frame

Filter media pack –

A continuous sheet

of filter media

ULPA Filters

Optional ULPA filters may be used to replace the standard HEPA filters in
the Purifier Cell Logic Biosafety Cabinets. ULPA filters have the same
properties as described above except they are rated at least 99.999% efficient
in removing particles 0.1-0.2 or 0.2-0.3 micron.

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Laminar Airflow

Laminar airflow is defined as the movement of a body of air in a single
direction, with a uniform velocity. In practice, the laminar downflow of air in
the cabinet captures any aerosol generated in the work area of the cabinet,
and directs it to the HEPA filters. In order to be true laminar downflow, a
number of individual downflow velocity test points (The Downflow Velocity
Profile) must be +/- 16 feet per minute (0.08 m/s) of the average of all the test
points. This is illustrated in Figure 4-2.

Figure 4-2

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Chapter 4: Performance Features and Safety Precautions

Directional Airflow

Directional airflow also plays a key role in biosafety cabinet performance.
Air is drawn into the front of the cabinet at the front grille. This “curtain” of
air makes it more difficult for aerosols to escape out of the work area of the
cabinet and into the outside environment. This airflow is often calculated and
referred to as the Inflow Volume or Average Inflow Velocity. This is
illustrated in Figure 4-3.

Figure 4-3

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Motor/Blower

The motor/blower assembly pulls room air into the front of the cabinet, and
re-circulates it internally. During its recirculation, the air is split into two
separate streams. One path leads through the exhaust HEPA filter and out of
the unit. The second path flows through the supply HEPA filter, which then
flows down through the work area, as shown in Figure 4-4. The motor in the
Purifier Cell Logic Cabinet is an electronically commutated motor (ECM).
The ECM is a brushless DC motor that includes its own power supply to
convert the incoming alternating current to direct current, as well as its own
microprocessor to control and measure the motor’s operation. The motor is
preprogrammed to deliver a consistent volume of air, even as the HEPA
filters load over time.

Figure 4-4

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Chapter 4: Performance Features and Safety Precautions

Cabinet Air Intakes (Grilles), Ductwork and Air
Balance Controls

The location, size, and pattern of the grilles at the front and rear of the work
area affect cabinet containment and performance.

Note: Never block or obstruct the grilles of the Biosafety Cabinet.

The internal ductwork of the Biosafety Cabinet conveys the air from the work
area to the blower, and then from the blower to the filters. The positive
pressure rigid plenum of the Biosafety Cabinet is designed to deliver a more
uniform airflow to both HEPA filters, optimizing filter loading and
operational life.

Ultraviolet (UV) Lamp

Note: The Ultraviolet (UV) lamp is not recommended or readily available for
Cell Logic Biosafety Cabinets with the Scope-Ready Package because the
sash cannot be safely closed with the microscope in place. Good surface
disinfection is the preferred method. Furthermore, the following factors
cause concern with UV and microscopes:

• The microscope creates a shadow where the UV light cannot reach.

• Removing the microscope is often undesirable.

• Running UV light with the microscope inside will degrade the

microscope.

The optional UV lamp generates a primary wavelength of light of 254nm. A
secondary emission is in the visible (blue) wavelength, resulting in the
characteristic blue color while operating. UV light with a wavelength of 254
nm is biocidal, primarily by creating thymine dimers in DNA. These dimers
prevent the correct transcription of the DNA into RNA, resulting in cellular
death or viral inactivation. In order to be effective, the UV light must directly
strike the nucleic acid, and its effectiveness can be diminished or negated by
dissolved proteins or metals, or by other UV-opaque substances protecting
the target nucleic acid.

Because of its limitations, UV light should be used as an adjunct to good
surface disinfection practices. In order to get optimum performance from the
UV light, it should be replaced after 6,000 hours of operation or less, and the
exterior surface of the lamp should be kept clean and free of dust.

Note: The Cell Logic Biosafety Cabinet records the number of hours of
operation of the UV light. You can program in the number of hours (in 100­hour increments) it will operate before a replacement message is displayed.

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Note: UV irradiation is absorbed by the tempered safety glass of the sash.
Independent research has shown that the level of UV irradiation on the
outside of the cabinet’s sash is equal to background radiation levels.

Note: The UV sensitivity of a target organism varies, depending on the UV
output of the lamp, the genus and species of the organism, the medium the
agent is suspended in, etc. Contact the facilities Health and Safety Officer at
your facility for UV light use and recommendations.

Scope-Ready™ Package Option

Models with the Scope-Ready package option allow a user-supplied
microscope to be integrated into the safety cabinet. On these models, two
features work together to assure containment and reduced vibration:

• The Pure-Vu™ Seal includes a 7" wide x 10.5" (267 mm) high sash
cutout that accommodates most microscopes’ eyepieces. The clear
disposable eyepiece seal prevents contaminants from escaping while
providing ergonomic visibility into the cabinet. The easy removable
seal attaches tightly to an aerodynamic, epoxy-coated steel collar.
The unique teardrop shape of the aerodynamic collar design keeps
contaminants contained with the microscope in place. The Pure-Vu
Seal conforms to both ASHRAE 110 and NSF 49 testing criteria.

• The Stand-Still™ Isolation Platform supports the microscope on the
cabinet’s work surface and removes up to 94% of vertical and
horizontal vibrations. ASHRAE 110 and NSF 49 testing confirms
this unique elevated, aerodynamic base ensures safe airflow.
Constructed of seamless Type 304 stainless steel, the isolation
platform is non-porous, easy to clean, and autoclavable. Important
Note: Always remove the rubber isolation feet prior to autoclaving.
The spacious 12" (305 mm) wide x 18.75 (476 mm) deep platform fits
most microscopes. See Appendix D: Accessories for ordering an
extended Stand-Still XL Isolation Platform for use with microscope
bases that extend over the front of the lower work surface.

Temp-Zone™ Package Option

Models with the Temp-Zone package option include a temperature controlled
area 10.5" (267 mm) wide x 12" (305 mm) deep (7" (178 mm) wide x 12"
(305 mm) deep on 3' models). Samples heated in an incubator or chilled in a
refrigerator may be transferred to the Temp-Zone and kept at optimum
temperature. Quick connection fittings on the left side of the cabinet attach
to a user-supplied circulator that continually delivers heated or chilled water
through channels located underneath the Temp-Zone area of the worksurace.
Uniform temperature distribution is maintained on the Temp-Zone area of the
work surface. Temp-Zone work area temperatures of 0.3-0.5°C are
maintained with the circulator heated to 37°C and within 0.3-1.6°C with the

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Chapter 4: Performance Features and Safety Precautions

circulator chilled to 3.7°C. Actual set points will vary depending on
variables such as sample tube rack, sample size, and length of tubing.

Safety Precautions

WARNING: The Biosafety Cabinet Biohazard Cabinet should be certified
by a certification technician before its initial use. The cabinet should be
recertified whenever it is relocated, serviced or at least annually thereafter.
Do not assume that filter integrity and airflow performance have not been
compromised during shipping.

Some internal components of the Biosafety Cabinet may become
contaminated during operation of the unit. Only experienced personnel
competent in decontamination procedures should decontaminate the cabinet
before servicing these components. If you have any questions regarding
certification agencies, or need assistance in locating one, contact Labconco’s
Product Service Department at 800-821-5525 or 816-333-8811.

DO NOT load more than 50 lbs. (23 Kg) in the work area. Exceeding this
limit may damage the work surface and its supports. Excessive weight in the
cabinet may increase the risk of it overturning, or failure of hydraulic lift
stands, resulting in the cabinet and stand overturning. If your application
requires loading more than 50 lbs., contact Labconco’s Product Service
Department at 800-821-5525 or 816-333-8811 for assistance.

Ensure that the Cabinet is connected to electrical service in accordance with
local and national electrical codes. Failure to do so may create a fire or
electrical hazard. Do not remove or service any electrical components
without first disconnecting the Biosafety Cabinet from electrical service.

Avoid the use of flammable gases or solvents in the Biosafety Cabinet. Care
must be taken to ensure against the concentration of flammable or explosive
gases or vapors. An open flame should NOT be used in the Biosafety
Cabinet. Open flames will disrupt airflow patterns, burn the HEPA filter
and/or damage the filter’s adhesive. Gases under high pressure should not be
used in the Biosafety Cabinet, as they may disrupt its airflow patterns.

HEPA filters only remove particulate matter. Operations generating volatile
toxic chemicals or radionuclides must be evaluated carefully.

The media of HEPA filters is fragile and should not be touched. Avoid
puncturing either HEPA filter during installation or normal operation. If you
suspect that a HEPA filter has been damaged, DO NOT use the cabinet;
contact a local certification agency or Labconco at 800-821-5525 or 816-333­8811 for re-certification information.

The HEPA filters in the Biosafety Cabinet will gradually accumulate airborne
particulate matter from the room and from work performed in the cabinet.
The rate of accumulation will depend upon the cleanliness of the room air,

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operating time and the nature of work being done in the cabinet. The Filter
Gauge accurately displays the amount of filter life remaining.

Proper operation of the cabinet depends largely upon its location and the
operator’s work habits. Consult the Installation and Normal Operation
sections of this manual for further details.

Avoid direct exposure of plastic or coated materials to ultraviolet (UV)
radiation. Never bypass the UV safety interlock that only allows the UV
light to work when the sash is closed. When surface disinfecting the
Biosafety Cabinet:

• Avoid splashing the disinfecting solution on skin or clothing.

• Ensure adequate ventilation.

• Carefully follow the disinfectant’s safety instructions.

• Always dispose of disinfecting solutions in accordance with local and

national laws.

• DO NOT allow disinfectants with high concentrations of free chlorine
to contact the stainless steel components of the Biosafety Cabinet for
a long period of time. Free chlorine will corrode stainless steel after
extended contact.

The electrical receptacle cover may be removed to facilitate surface
decontamination. In the event of gross contamination, the cover should
be removed, sterilized and/or decontaminated as required and discarded.
Order Replacement Receptacle Cover #1287900. See Appendix A:
Components for a list of service parts.

Biosafety Cabinets should be decontaminated for any of the following
reasons:

• Before maintenance work requiring entry into contaminated areas.

• Before HEPA filter changes.

• Before performing certification tests requiring entry into

contaminated areas.

• Before relocating the cabinet.

• Before changing research programs.

• After the gross spill of biohazardous material or toxic chemicals.

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CChhaapptteerr 55::
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System Reset Switch

The Biosafety Cabinet has a system reset switch for resetting its
microprocessors. The switch is located on the front of the electronics module,
on top of the Cabinet, as shown in Figure 5-1. Ensure that the switch is in the
“ON” (up) position before attempting to operate the Cabinet.

The System Reset
Switch

Figure 5-1

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Information Center

The Information Center is an LCD display located on the right side wall at
eye level. The center’s display is divided into three separate areas, as shown
in Figure 5-2, below. The display will turn itself off one minute after the
blower is turned off or the sash is closed.

Status Area

Data Area

Icon Area

Figure 5-2

Status Area

The Status Area displays the system’s condition during operation. This line
can display any of the following messages:

Blower Off

The cabinet blower is shut off.

Please Wait

For three minutes after the cabinet blower is turned on, the display will
say this, to allow the cabinet to reach proper operating conditions.

OK

The motor is operating properly, and the sash is not open too far.

Night-Smart™

If the Night-Smart mode is selected, as outlined later in the chapter, this
message will appear for one minute after the sash is closed, and then the
display will automatically shut off.

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Chapter 5: Using The Cabinet

Sash is too high

The sash is open too far for safe operation.

Airflow Alert

The airflow patterns in the cabinet have changed, resulting in a sudden
change in the motor speed. This is most likely due to a blockage of the
grille or the exhaust filter outlet. It may also be caused by removal of the
work surface while the cabinet is in operation.

System Error

The motor and display circuit board are not communicating properly.
DO NOT USE THE CABINET UNTIL THE PROBLEM HAS BEEN
CORRECTED.

Data Area

The Data Area displays the Filter Gauge. This bar graph displays the percent
of remaining filter life. When the cabinet is new, or after the HEPA filters are
replaced, the gauge will be “full”, or display approximately 100%. As the
filters load, or if the grille or exhaust discharge are blocked, the bar graph
will show a reduced filter life.

The graph is user-selectable as a bar graph with percentage displayed, a bar
graph only, or a semi-circular graph showing filter life remaining.

Various factors such as temporary obstructions to airflow, temperature and
barometric pressure can cause the displayed percentage to vary slightly.
Ignore short term changes in the Filer Gauge’s value. As the Filter Gauge
approaches 20% remaining capacity, consider having the cabinet filter
replaced.

When the optional airflow sensor is installed and calibrated, the inflow and
calculated downflow velocities are also displayed during operation.

Icon Area

The Icon Area displays icons for those features selected or in operation. The
Icons are:

Normal blower operation –

In this mode the cabinet blower is operating normally.

Night-SmartTM blower operation

When Night-Smart
when the sash is closed, to maintain the cleanliness of the air in
the work area. When the sash is opened, the blower resumes
normal operation.

is selected, the blower slows to idle speed

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Fluorescent light

UV light

Timer

Security Lock

Mute

Chapter 5: Using The Cabinet

In this mode the fluorescent lights are operating. When the sash is
fully closed, the fluorescent lights automatically turn off.

In this mode the UV (germicidal) light is operating. When the
sash is fully closed, the UV light can be programmed to
automatically turn on. When the sash is raised, the light
automatically turns off, to protect the laboratory from UV
irradiation. Note: The UV light feature is not recommended or
readily available for Cell Logic Biosafety Cabinets with the
Scope-Ready Package.

When either the interval or stopwatch timer mode is active, this
icon appears.

When the security lock is activated the keypad does not respond to
any buttons until the proper sequence of keystrokes is entered.

When this icon is on, the audible alarm is muted for
approximately 5 minutes.

Operating the Sliding Sash

The counterbalanced, anti-racking sash mechanism requires only a few
pounds of force to move the sash up or down. You can open or close the sash
smoothly with one or two hands positioned on either handle.

Note: Cell Logic Biosafety Cabinets with Scope-Ready Package will not
allow for easy movement of the sash with the microscope eyepieces installed.
The blower and safety interlock system will typically work in manual mode
for these models unless the microscope is disengaged from the Pure-Vu Seal.

The sash position alarm and safety interlock system senses the sash position
and acts appropriately. The Cell Logic Biosafety Cabinet has been
programmed to operate at a 9-inch (229 mm) sash opening, depending on
model. Raising the sash above its operating height will activate the audible
and visual alarms. The audible alarm can be temporarily muted (for
approximately five minutes) by depressing and releasing the Mute/OK
button. Closing the sash back to its operating position will reset the alarm and
defeat the muting of the alarm. The safety interlock system senses when the
sash is closed and allows the optional ultraviolet (UV) lamp to operate only
when the sash is closed, to protect the operator from irradiation.

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Starting the Biosafety Cabinet

1. To start the Biosafety Cabinet, raise the sash until its bottom edge
aligns with the sash position label on the left corner post. The decal is
shown in Figure 5-3.

2. Press the blower button to start the unit. The unit will run a self-test
for approximately 15 seconds. Additionally, you will be instructed to
“Please Wait” for three minutes to allow the cabinet to reach proper
operating conditions. If the alarm sounds, recheck the sash position.
If the sash is too high, the sash audible alarm and the LCD display
will indicate the sash is too high.

3. To turn the UV light on, the sash must be completely closed to
prevent the escape of any UV radiation. Push the UV light button to
activate the UV light.

Note: The sash must be completely closed for the UV light to activate.

Sash position label

Figure 5-3

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The Logic Touchpad

The touchpad of the Cell Logic Biosafety Cabinet is shown in Figure 5-4.
Take a moment to get familiar with the buttons, their location and function.
Also familiarize yourself with the display located on the right side wall. The
display will report system functions, such as filter capacity, timer displays,
alarm or error messages, as well as icons that illuminate when cabinet
functions such as UV light and blower are operational

Blower Button – Starts or stops the cabinet blower. When
the blower is in Smart-StartTM mode, closing the sash turns
the blower off. When the sash is raised, the blower restarts
automatically. In Night-SmartTM mode, when the sash is
closed, the motor slows to idle to maintain air cleanliness in
the work area. When the sash is reopened, the blower
resumes normal operation. Pressing this button overrides
Smart-StartTM and Night-Smart

TM

operation.

Light Button – Turns the fluorescent lamps on or off.
Closing the sash automatically turns the lights off. When
the lights are in Smart-StartTM mode, raising the sash turns
the lights on automatically.

UV Light Button – Turns the UV lamp on or off. When
the UV lamp is in Smart-StartTM mode, closing the sash
turns the light on. When the sash is raised, the light turns
off automatically.

Timer Button

– Allows you to select either a repeating

interval timer, or an elapsed timer (stopwatch).

Mute/OK Button

– Mutes all audible alarms for

approximately 5 minutes, unless it is a system error alarm.
When in the Menu mode, this button is used to select an
option.

Menu Button

– This button toggles the display between

the display and menu modes. When in the menu mode,
pressing this button returns you to the previous menu level.

Select Buttons – Allow you to choose different options in
the menu mode.

Figure 5-4

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Chapter 5: Using The Cabinet

Figure 5-5

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Chapter 5: Using The Cabinet

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Chapter 5: Using The Cabinet

User Selectable Features

The Purifier Cell Logic Biosafety Cabinet offers the user unparalleled
flexibility and convenience. The operation of the blower, fluorescent and UV
lights can either be operated manually, or you may choose to use the Smart­Start™ or Night-Smart™ features that activate functions automatically when
the sash is opened or closed.

The UV lamp can be programmed to operate for a given time interval when
the sash is closed, before it shuts off.

NOTE: During the first fifteen seconds of operation, the Cabinet
performs an auto-diagnostic routine to check the operation of the
microprocessor. You cannot access the menu during this time.

NOTE: When you are in the menu mode, if a selection is not made within
30 seconds, the display will reset back to display mode.

NOTE: Pressing the appropriate touchpad button will override Smart­StartTM or Night-SmartTM selections.

To access the menu, press the “MENU” button. The display panel will
show the first level menu. To select from the various menu options press
the “▲” or “▼” buttons until the selected option is displayed. Press
“OK” to accept that option, or press “MENU” to return to the previous
menu level.

For further explanation of the menu and its options, please refer to figure 5-4.

Timer Operation

NOTE: The timer button allows activation of an interval (countdown) or
stopwatch (elapsed) timer. The timers cannot be operated
simultaneously.

To access the main timer menu, press the “Timer” button anytime during
normal operation. The main timer menu is shown on the LCD display. Use
the “▲” and “▼” buttons to highlight the Interval or Stopwatch Timer. Press
the “OK” button to select the highlighted timer function.

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Interval Timer Operation

1. When selected, the Interval Timer menu is displayed on the LCD.
The timer defaults to 0:00:00 (hours:minutes:seconds).

2. Press and hold the “▲” or “▼” buttons to increase or decrease the
timer interval.

3. When the proper interval is entered on the display, press the “OK”
button to start the timer.

4. When the timer reaches 0:00:00, an audible alarm will sound, and the
timer will reset itself and repeat the countdown .

5. Press the “OK” button to pause the timer.

6. Press the “Menu” button to clear the interval timer and return to the
main timer menu.

Stopwatch Timer Operation

1. When selected, the Stopwatch Timer menu is displayed on the LCD.
The timer defaults to 0:00:00.

2. Press the “OK” button to start the timer.

3. Press the “OK” button again to zero the timer.

4. Press the “Menu” button to clear the stopwatch timer and return to the
main timer menu.

Menu Options

UV Lamp Operation

This menu option allows you to select whether the UV light will turn on
automatically every time the sash is completely closed.

Smart-Start

When the “Smart-Start” option is selected, the UV light will turn on
every time the sash is closed.

Manual

When the “manual touchpad” option is selected, the UV light must be
turned on and off at the touchpad.

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UV Lamp Timer

The UV Lamp Timer allows you to decide whether the UV lamp will operate
continuously or for a time period that you select.

Interval

When the “Interval” option is selected, the UV Interval Timer menu is
displayed.

UV Interval Timer

This window lets you set the amount of time the UV lamp
stays lit when activated. Use the “▲” and “▼” buttons to
select the amount of time you need, then press the “OK”
button to set the interval.

NOTE: Raising the sash will reset the timer to its original
setting. Pressing “UV Light” will turn the light off,
pressing “Mute/OK” will pause the timer.

Continuous

In the continuous mode, the UV light will remain lit until the “UV Light”
button is pressed, or the sash is raised.

UV Lamp Maintenance

This selection allows you to check how many hours the UV lamp has burned.
It also allows you to set the number of hours you want the lamp to last, and to
reset the UV lamp hourmeter.

UV Lamp Hourmeter

This display only shows how many hours the UV lamp has been lit, and
how many hours remain until you will receive a warning to replace the
lamp.

Change UV Lamp Life

In this option, you can set the number of operating hours before
receiving the replace UV lamp warning. For most UV lamps, the output
of UV light decreases at a constant rate. Typically, after 6,000 hours of
operation the lamp will output 80% of the UV light it did when it was
new. This option allows you to set the warning at a life you prefer, in
100 hour increments.

Reset UV Lamp Clock

This option lets you reset the UV hourmeter to 0 whenever the lamp has
been replaced.

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Light Operation

This menu option allows you to select whether the fluorescent lights will turn
on automatically every time the sash is opened.

NOTE: The fluorescent lights will automatically turn off when the sash is
closed, no matter what its setting is.

Smart-Start

When the “Smart-Start” option is selected, the fluorescent lights will
turn on every time the sash is opened.

Manual

When the “manual touchpad” option is selected, the fluorescent lights
must be turned on and off at the touchpad.

Light Timer

The fluorescent Lamp timer allows you to decide whether the fluorescent
lamps will operate continuously or for a limited time period that you select.
This may be desired for energy-savings as well as a safeguard for light­sensitive materials or animals.

Interval

When the “interval” option is selected, the Light Interval Timer menu is
displayed.

Light Interval Timer

This window lets you set the amount of time for the fluorescent
lamps to stay lit once activated. Use the “▲” and “▼” buttons to
select the amount of time, then press the “OK” button to set the
interval.

NOTE: Closing the sash will reset the timer to its original
setting. Pressing “Light” will turn the lights off, pressing
“Mute/OK“ will pause the timer.

Continuous

In the continuous mode, the fluorescent lights will remain lit until
the “light” button is pressed, or the sash is closed.

Light Maintenance

This selection allows you to check how many hours the fluorescent lamps
have burned. It also allows you to set the number of hours before replacement
and to reset the light hourmeter.

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Chapter 5: Using The Cabinet

Light Hourmeter

This display only shows the hours the fluorescent lamps have been lit
and how many hours remain until you receive a warning to replace the
lamps.

Change Light Life

In this option, you can set the hourmeter before you get the replace light
warning. For most fluorescent lamps, the output of light decreases at a
constant rate. Typically, after 6,000 hours of operation the lamp will
output less light than it did when it was new. This option allows you to
set the warning at what ever level you prefer, in 100 hour increments.

Reset Light Clock

This option resets the light hourmeter to 0 after the fluorescent lamps
have been replaced.

Blower Operation

This selection allows you to choose to operate the blower in Smart-Start,
Manual Touchpad, or in Night-Smart modes.

Smart-Start

When the “Smart-Start” option is selected, the blower will turn on every
time the sash is opened. When the sash is closed, the blower will shut
off.

Manual Touchpad

When the “Manual Touchpad” option is selected, the blower must be
turned on and off at the touchpad.

Night-Smart

When the “Night-Smart” option is selected, the blower will turn on every
time the sash is opened. When the sash is closed, the blower speed will
decrease, to deliver 20-30% of the volume of air during normal
operation.

Cabinet Set Up

These selections allow for the customization of the display and the cabinet
operation.

Units of Measure

When equipped with the optional airflow sensor, this option allows you
to select the air velocity units of measure. When “Metric” is selected, the
air velocities will be displayed in meters-per-second. When “US” is
selected, the velocities will be displayed in feet-per-second.

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Chapter 5: Using The Cabinet

Security Lock

NOTE: The security lock automatically reactivates and locks out
the keypad every time the blower is shut off, by the keypad or by
closing the sash. After the initial auto diagnostic screen is cleared,
the security lock reactivates.

The security lock feature prevents operation of the Biosafety Cabinet by
unauthorized users. To engage the security lock, access the Security
Lock submenu, as described in Figure 5-4. Select the “secure” option,
and press the “OK” button. A ‘lock’ icon appears on the LCD display.
Once engaged, the operator must enter the proper sequence of keystrokes
to operate the cabinet. The proper sequence to unlock the Security Lock
is:

Press the “▼” keypad 3 times within 2 seconds.

After successful entry of the unlock code, the lock icon disappears from
the display, and the user has full access to the keypad functions.

To disable the Security Lock, enter the Security Lock submenu and
select the non-secure mode. The lock is completely disabled.

Filter Life Display Options

This menu selection determines how the filter life remaining gauge is
displayed. When “Bar Graph with %” is selected, the gauge will be
displayed as a horizontal bar graph with the filter life remaining shown
graphically and digitally. When “Circle Graph” is selected, the gauge is
displayed as a semicircular bar graph, including a digital display.

Data Output Rate

Note: This selection will only work if the optional RS-232 board is
installed.

This menu option selects the rate that the RS-232 board outputs data.
Data can be output at a rate of once per second, once every 10 seconds,
once every 30 seconds, or once per minute.

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Chapter 5: Using The Cabinet

Restoring Software Default Settings

To restore the default settings to their original configuration:

1. Access the Menu, and select “Cabinet Set Up”. Press “Mute/OK”

2. In the Cabinet Set Up submenu, select “Restore Defaults”. Press
“Mute/OK”

3. Press “Mute/OK” again to restore the software defaults. Pressing
“Menu” will escape this submenu without restoring the defaults.

Table of Default Settings

UV Lamp: Smart-Start, Continuous, Reset UV Lamp

Hourmeter, Set UV Lamp Life to 6000 hours.

Fluorescent Lamp: Smart-Start, Reset Lamp Hourmeter, Set

Fluorescent Lamp Life to 6000 hours.

Blower: Smart-Start

Units of Measure: Ft / Min

Security Lock: Non-secure

Data Output Rate: Every second via optional RS-232 Board

Calibration

This selection is for the use of qualified certifiers only to adjust the
blower speed, or the display of the filter life remaining gauge.

Diagnostic Test Operation

When the “Diagnostic Test” menu is selected, the LCD allows keypad/
hardware testing to help troubleshoot any malfunction.

Keypad Testing

When keypad testing is selected, the LCD shows when each key is
pressed, to confirm the touchpad is working. In addition, when the
“Light” and “UV Light” keys are pressed, the associated relays are
energized, so the lamps should work. The Timer button energizes an
auxiliary relay. When “Mute/OK” is pressed, the audio alarm should
work. When the “▲” key is pressed, the Airflow Alarm relay is
energized. When the “▼” key is pressed, the Blower On relay will be
energized. Finally, the sash position switches can be checked in this
mode as the LCD displays the sash position information that the
controller is receiving.

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Chapter 5: Using The Cabinet

If An Airflow Alert Activates

The most common causes of an Airflow Alert are:

• Blockage of the inlet grilles or exhaust outlet.

• Removal of the work surface or grille during operation.

Resetting the Airflow Alert System

The Airflow Alert automatically resets to normal operation once the motor
speed has stabilized.

Working In the Biosafety Cabinet

Planning

• Thoroughly understand procedures and equipment required before
beginning work.

• Arrange for minimal disruptions, such as room traffic or entry into the
room while the cabinet is in use.

Start-up

• Turn off UV light if included.

• Slowly raise the sash until the bottom of the sash aligns with the sash

indicator decal located on the left side of the work area.

• Turn on the fluorescent light and cabinet blower if the Smart-Start
features have not been activated.

• Check the air grilles for obstructions, and note the filter gauge
reading.

• Allow the cabinet to operate unobstructed for 5 minutes.

• Wash hands and arms thoroughly with germicidal soap.

• Wear a long sleeved lab coat with knit cuffs and over-the-cuff rubber

gloves. Use protective eyewear. Wear a protective mask if
appropriate.

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Chapter 5: Using The Cabinet

Wipe-Down

• Raise the sash to its full open position (approximately 21.75 inches or
552 mm). Mute the alarm by depressing the "Alarm Silence" switch.

• Wipe down the interior surfaces of the cabinet with 70% ethanol, or a
suitable disinfectant, and allow to dry.

Loading Materials and Equipment

• Only load the materials required for the procedure. Do not overload
the cabinet.

• Do not obstruct the front, side, or rear return air grilles.

• Large objects should not be placed close together.

• Slowly close the sash until it is in the correct operating position.

• After loading the cabinet, wait two to three minutes to purge airborne

contaminants from the work area.

Work Techniques

• Keep all materials at least 4 inches (100 mm) inside from the sash,
and perform all contaminated operations as far to the rear of the work
area as possible.

• Segregate all clean and contaminated materials in the work area.

• Arrange materials to minimize the movement of contaminated

materials into clean areas.

• Keep all discarded contaminated material to the rear of the work area.

• Avoid moving materials or the operator's hands and arms through the

front access opening during use.

• Avoid the use of an open flame. Use disposable lab ware or an
electric incinerator as alternatives.

• Use proper aseptic technique.

• Avoid using techniques or procedures that disrupt the airflow patterns

of the cabinet.

• If there is a spill or splatter during use, all objects in the cabinet
should be surface decontaminated before removal. Thoroughly
disinfect the working area of the cabinet WHILE IT IS STILL IN
OPERATION, to prevent the release of contaminants from the
cabinet.

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Chapter 5: Using The Cabinet

Final Purging

• Upon completion of work, the cabinet should be allowed to operate
for two to three minutes undisturbed, to purge airborne contaminants
from the work area.

Unloading Materials and Equipment

• Objects in contact with contaminated material should be surface
decontaminated before removal from the cabinet.

• All open trays or containers should be covered before being removed
from the cabinet.

Wipe-Down

• Wipe down the interior surfaces of the cabinet with 70% ethanol, or a
suitable disinfectant, and allow to dry.

• Periodically lift the work surface and wipe down the area beneath it.

• Inspect and clean the towel catch located at the rear of the work area,

beneath the work pan.

• Dispose of rubber gloves appropriately, and have lab coat laundered
properly.

• Wash hands and arms thoroughly with germicidal soap.

Shutdown

• Lower the sash to turn off the fluorescent light and cabinet blower.

Note: Cell Logic Biosafety Cabinets with Scope-Ready Package will
not allow for easy movement of the sash with the microscope
eyepieces installed. The blower and safety interlock system will
typically work in manual mode for these models unless the
microscope is disengaged from the Pure-Vu Seal.

• If appropriate for Cell Logic Biosafety Cabinets with Temp-Zone
Package, activate the UV light.

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CChhaapptteerr 66::
MMaaiinnttaaiinniinngg tthhee CCaabbiinneett

The common service operations necessary to maintain the Biosafety Cabinet
for peak performance are listed below.

Note: This manual covers operation and maintenance operations for the
owners/users of the Cell Logic Biosafety Cabinets. Complete certification
procedures, service operations and specifications are published in a separate
publication Technical Manual: Purifier® Logic® Biosafety Cabinets and

PuriCare

website: www.labconco.com. A complete certifier service kit is available to
qualified certifiers from Labconco. Call Labconco’s Product Service
Department at 800-821-5525 or 816-333-8811.

®

Procedure Stations. This manual is available from Labconco’s

Routine Maintenance Schedule

Weekly

• Using 70% ethanol, or a suitable disinfectant, surface disinfect the
inside of the cabinet and the work surface.

• Using an appropriate glass cleaner, or LabSolutions Glass & Surface
Wipes, Labconco part # 1570000; clean the sash and the surface of
the UV lamp, if so equipped.

• Operate the cabinet blower, noting the percent filter life remaining in
an operational log.

Monthly (or more often as required)

• Using a damp cloth, or LabSolutions Glass & Surface Wipes,
Labconco part # 1570000; clean the exterior surfaces of the cabinet,
particularly the front and top of the cabinet, to remove any
accumulated dust.

• Disinfect and lift the work surface. Surface disinfect the lower
plenum with a solution of 70% ethanol, or a suitable disinfectant.
Check the towel catch for retained materials.

• Check all service valves, if so equipped, for proper operation.

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Chapter 6: Maintaining The Cabinet

• Check the UV and fluorescent light hourmeters, and record their
readings in an operational log.

• All weekly activities.

Semiannually or Annually

• Have the cabinet re-certified by a qualified certification technician.

• All monthly activities.

Service Operations

Work Surface Removal:

Note: The work surface of the cabinet must be thoroughly decontaminated

before removing it.

Note: The Temp-Zone package option has a prop rod on both sides to aid in
pivoting and cleaning. The Temp-Zone work surface requires disconnection
of the tubing from the quick connect fittings on the left side of the cabinet
before removal.

1. Lift the front edge of the work surface straight up by grasping the knob
handles at either front corner.

2. Pull the work surface straight out, letting its rear edge rest on the center
support underneath.

3. Reinstall the work surface by resting the bottom on the center rail while
pushing it back into the cabinet. Be sure to engage the tabs on the back
corners of the work surface with the slots on the rear wall of the work
area.

Front Grille Removal:

Note: The grille must be thoroughly decontaminated before removing it.

1. Remove the work surface as described earlier.

2. At one end of the grille, grip the front of grille with one hand, and the
back with the other hand. Pivot that end of the grille upward and
inward, paralleling the angle of the sash, as shown in Figure 6-1.

3. Pull the other end of the sash up and away from the bottom edge of the
cabinet.

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Chapter 6: Maintaining The Cabinet

4. Reinstall the grille by reversing the above sequence, ensuring that the
grille properly engages the bottom edge of the cabinet.

Figure 6-1

Towel Catch Removal:

Although not normally required, the towel catch can be removed for cleaning,
inspection, etc.

Note: The work surface of the cabinet and the towel catch must be
thoroughly decontaminated before removing either.

1. Remove the work surface as described above.

2. Lift the front end of the center support straight up to release it from the
locating pins (A). Set the support aside in the work area.

3. Remove the towel catch by pivoting the bottom out toward you (B).

4. Surface decontaminate the towel catch before removing it.

5. Reinstall the towel catch by sliding it back into position, ensuring that the
two slots on either end of the towel catch properly engage the rails on the
rear of the duct, as shown in Figure 6-2. Also ensure that the DOP
Sampling Line (the clear tube with a black cap) either passes through a
hole in the towel catch, or passes along the outside edge of the catch
without being kinked.

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Chapter 6: Maintaining The Cabinet

Front Panel Removal and Installation:

Figure 6-2

Figure 6-3

1. Locate and remove the two Phillips
screws that secure the front panel as
shown in Figure 6-3. They are
located on the bottom corners of the
front dress panel.

2. Swing the bottom of the dress panel
out to clear the fluorescent light and
then lift the front dress panel
straight up and away from the
cabinet.

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Chapter 6: Maintaining The Cabinet

1. To reinstall the panel, reverse these
steps, ensuring that the plastic pin
in the top corners of the dress panel
properly engage the corner posts.

Changing the Fluorescent Lamps:

Figure 6-4

1. Unplug the cabinet or turn off the System Reset Switch located on the top
of the cabinet.

2. Remove the front dress panel as noted in Figure 6-3.

3. Remove the fluorescent lamps by pulling the lamp sockets straight off
each end of the lamp, and releasing both lamps from the spring clips that
secure them in place.

4. Install the new lamps by reversing the removal procedure.

Changing the Optional UV Lamp:

Note: For optimum performance, the UV lamp should be changed on an
annual basis.

The UV lamp and the work area of the cabinet must be thoroughly
decontaminated before removing the lamp.

1. Start the cabinet and let it operate for 5 minutes.

2. Raise the sash to its full open position.

3. Thoroughly surface decontaminate the UV lamp and the work area of the
cabinet.

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Chapter 6: Maintaining The Cabinet

4. Unplug the cabinet or turn off the System Reset Switch, located on the
top of the cabinet.

5. Remove the UV lamp by rotating it 90 degrees and lifting it straight up
and out of its sockets.

6. Install new lamp by reversing the removal procedure.

Resetting a Circuit Breaker:

To reset any of the circuit breakers located on the left side of the electronics
module, depress the white button until it sets.

Circuit Breakers- The
front breaker protects
the electrical outlets,
the rear breaker
protects the motor and
lights.

Figure 6-5

Storage

If the Biosafety Cabinet is to be left unused for more than one month, it
should be prepared for storage.

Note: The cabinet should not be stored in areas of excess humidity or
temperature extremes. If the cabinet is moved during storage, it must be
recertified before use.

1. Close the sash completely and seal the bottom edge and the exhaust outlet
with plastic sheeting.

2. Unplug the cabinet.

3. Ensure that the cabinet will not be moved or disturbed while being stored.

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CChhaapptteerr 77::
TTrroouubblleesshhoooottiinngg

Refer to the following table if the Biosafety Cabinet fails to operate properly.
If the suggested corrective actions do not solve the problem, contact
Labconco for additional assistance.

PROBLEM CAUSE CORRECTIVE ACTION

Cabinet blower and
lights won’t turn on

Check connection to control box on top

System Reset Switch

Circuit breaker(s)

Keypad disconnected

Blower won’t turn on
but lights work

Blower wiring is

Blower motor is

Keypad disconnected

Unit not plugged into
outlet

is Off

tripped

or defective

Sash closed Raise sash.

disconnected

defective

or defective

Plug the Biosafety Cabinet into
appropriate electrical service.

of Cabinet.

Turn on the System Reset Switch.

Reset circuit breakers.

Run keypad diagnostics and check
connections.

Inspect blower wiring.

Replace blower motor.

Run keypad diagnostics and check
connections.

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Chapter 7: Troubleshooting

PROBLEM CAUSE CORRECTIVE ACTION

Fluorescent light not
working

Lamp(s) are defective Replace defective lamp(s)

Lamp wiring is

Defective lamp

Keypad disconnected

Fluorescent light is
dim or flickering

UV light not
working

Lamp is defective Replace defective lamp.

Lamp wiring is

Defective lamp

Keypad disconnected

UV light is dim or
flickering

Sash is closed Open sash – Fluorescent lights will not

work with the sash closed.

Inspect lamp wiring.

disconnected

Replace lamp ballasts.

ballasts

Run keypad diagnostics and check

or defective

Lamp(s) are defective Replace defective lamp(s)

Lamp wiring is
disconnected

Defective lamp
ballast

Sash is open Close sash – UV light will not work

disconnected

ballast

or defective

Lamp is defective or
is at end of operating
lifetime.

Lamp wiring is
disconnected

Defective lamp
ballast

connections.

Inspect lamp wiring.

Replace lamp ballast.

with the sash open.

Inspect lamp wiring.

Replace lamp ballast.

Run keypad diagnostics and check
connections.

Replace defective or worn out lamp.

Inspect lamp wiring.

Replace lamp ballast.

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Chapter 7: Troubleshooting

PROBLEM CAUSE CORRECTIVE ACTION

Airflow Alert goes
off and/or there is a
slight decrease in
filter life remaining
gauge

Blockage of the

Blockage of the

Blockage or

Contamination of
work in the cabinet

Restriction of the

External factors are

Cabinet is out of

HEPA filter loading The gauge reading steadily decreases as

the Cabinet is used.

Check all return air slots and grilles to
return air slots or
grille

exhaust outlet

restriction under the
work surface

Improper technique
or procedure for the
Biosafety Cabinet

return air slots or
grille – blockage of
the exhaust outlet

disrupting the cabinet
airflow patterns or
acting as a source of
contamination

adjustment/HEPA
filter(s) are defective

ensure that they are not blocked or

restricted.

Ensure that the exhaust outlet is not

blocked or restricted.

Ensure that the towel catch and plenum

beneath the work surface are

unobstructed.

See “Use of the Cabinet” section in the

manual.

Ensure that all return air slots, grilles

and the exhaust outlet are unobstructed.

See “Installation” section of this

manual.

Have cabinet recertified.

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AAppppeennddiixx AA::
CCoom

Illustration A-1 indicates the location of the following service parts, and
replacement accessory parts:

Biosafety Cabinet Replacement Parts

Item Quantity Part No. Description

1 1 3837201 Postfilter for 4-foot models (optional)
1A 2 3837202 Postfilters for 5-foot models (optional)
1B 2 3837203 Postfilters for 6-foot models (optional)

2 1 3838500 Exhaust HEPA Filter 3-ft
2A 1 3838501 Exhaust HEPA Filter 4-ft
2B 1 3838502 Exhaust HEPA Filter 5-ft
2C 1 3838503 Exhaust HEPA Filter 6-ft

3 1 3838400 Supply HEPA Filter 3-ft
3A 1 3838401 Supply HEPA Filter 4-ft
3B 1 3838402 Supply HEPA Filter 5-ft
3C 1 3838403 Supply HEPA Filter 6-ft

4 2 9721901 Lamp, Fluorescent, 3-ft
4A 2 9721900 Lamp, Fluorescent, 4-ft
4B 2 9721903 Lamp, Fluorescent, 5- & 6-ft

5 1 1271300 Lamp, UV (models with UV light only)

6 2 1287900 Receptacle Cover

7 14 1885404 Screw, #6-32 x 0.25" PH (for Pure-Vu Seal – not

mppoonneennttss

shown, see Figure 3-5)

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Appendix A: Components

1

2

3

4

5

6

A-1

58

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*

AAppppeennddiixx BB::

meennssiioonnss

DDiim

All dimensions in inches.

B-1

Width “A” “B” “C”

3' 42.3 36.5 13.6

4' 54.3 48.5 19.9

5' 66.3 60.5 26.9

6' 78.3 72.5 32.9

*When equipped with Postfilter

68.5" WITH
CANOPY CONNECTION

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Appendix B: Dimensions

B-2

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AAppppeennddiixx CC::
SSppeecciiffiiccaattiioonnss

Electrical Data

Cabinet Model Electrical Requirements

34309 Series 115 VAC – 60 Hz, 1 Phase – 12 Amps
34409 Series 115 VAC – 60 Hz, 1 Phase – 12 Amps
34509 Series 115 VAC – 60 Hz, 1 Phase – 16 Amps
34609 Series 115 VAC – 60 Hz, 1 Phase – 16 Amps

Motor Specifications

Cabinet Model Electrical Requirements

All 3- and 4-foot
Cabinets, all Voltages

Cabinet Model Electrical Requirements

All 5- and 6-foot
Cabinets, all Voltages

Electronically Commutated Motor (ECM)
120-277 VAC – 50/60 Hz,

7.7 Full Load Amps @115VAC

4.3 Full Load Amps @230VAC
1/2 H.P. 1500 RPM maximum
Automatic Thermal Protection

Electronically Commutated Motor
120-277 VAC – 50/60 Hz,

9.6 Full Load Amps @115VAC

6.8 Full Load Amps @230VAC
3/4 H.P. 1500 RPM maximum
Automatic Thermal Protection

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Appendix C: Specifications

Environmental Conditions

• Indoor use only.

• Maximum altitude: 12000 feet (3657 meters).

• Ambient temperature range: 41° to 104°F (5° to 40°C).

• Maximum relative humidity: 80% for temperatures up to 88°F

(31°C), decreasing linearly to 50% relative humidity at 104°F (40°C).

• Main supply voltage fluctuations not to exceed ±10% of the nominal
voltage.

• Transient overvoltages according to Installation Categories II
(Overvoltage Categories per IEC 1010). Temporary voltage spikes on
the AC input line that may be as high as 1500V for 115V models and
2500V for 230V models are allowed.

• Used in an environment of Pollution degrees 2 (i.e., where normally
only non-conductive atmospheres are present). Occasionally,
however, a temporary conductivity caused by condensation must be
expected, in accordance with IEC 664.

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AAppppeennddiixx DD::
AAcccceessssoorriieess

Note: Accessories marked with an asterisk (*) require a qualified
certifier to install and/or calibrate before use.

Telescoping Base Stands

These stands are available with either fixed feet or caster wheels. The user
sets the height of these stands before installation. The height can be set from

27.5 to 33.5 inches in 1-inch intervals, providing a cabinet work surface
height of 30.0 to 36.0 inches. The Base Stands for each Purifier Cell Logic
Series are listed below.

Width Cell Logic Series # Base Stand w/Feet # Base Stand w/wheels #

3' 34309-xx 3730300 3730310
4' 34409-xx 3730400 3730410
5' 34509-xx 3830500 3830510
6' 34609-xx 3730600 3730610

Telescoping Base Stand Shelf Kit (# 3850100)

This 20 x 8-inch long shelf is specifically designed to hold the Aspirator
Pump or other small accessories, and attaches to the lower right side of the
base stand. The kit includes shelf, braces and self-drilling fasteners. NOTE:

A power drill with a 5/16-inch socket is needed to attach the fasteners to
the base stand.

Manual or Electric Hydraulic Lift Base Stands

These base stands offer infinitely adjustable height between 25.5 and 33.5
inches, giving a cabinet work surface height of 28.0 to 36.0 inches. The
height is adjusted either by a manual (hand crank) or electric pump that
drives hydraulic rams in the legs of the stands. All of the hydraulic stands are
equipped with fixed feet, but can be converted to caster wheels with the
addition of Caster Kit #3784000. The Base Stands for each Purifier Cell
Logic Series are listed below.

Width Cell Logic Series # Manual Lift Stand #

3' 34309-xx 3780200 3780100 3780103
4' 34409-xx 3780201 3780101 3780104
5' 34509-xx 3780203 3780106 3780107
6' 34609-xx 3780202 3780102 3780105

Electric (115V) Lift

Stand #

Electric (230V)

Lift Stand #

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Appendix D: Accessories

Hydraulic Base Stand Shelf Kit (# 3850200)

This 22 ¼ x 11 ¼-inch long shelf is specifically designed to hold the
Aspirator Pump or other small accessories, and attaches to the lower left side
of the base stand. The kit includes shelf and self-drilling fasteners. NOTE:

A power drill with a 5/16-inch socket is needed to attach the fasteners to
the base stand.

SoLo Electric Hydraulic Lift Base Stands

These base stands permit the Logic to be lowered enough to be transferred
through a standard doorway as low as 78 inches. Casters provide mobility
and lock in place. The SoLo Stands for each Purifier Cell Logic Series are
listed below.

Width Cell Logic Series # 115V SoLo Stand # 230V SoLo Stand #

3' 34309-xx 3780310 3780314
4' 34409-xx 3780311 3780315
5' 34509-xx 3780312 3780316
6' 34609-xx 3780313 3780317

*Seismic Bracket Kit (# 3857000)

Stainless steel brackets and attaching hardware to secure Telescoping Base
Stands in areas of seismic activity (does not include fasteners to attach to
floor).

*Service Valve Kit (# 3747500)

Includes serrated hose tip ball valve with quarter turn handle, hardware and
instructions for plumbing to services mounts on left or right side interior. All
cabinets are factory prepared to accept up to 4 fixtures. Shipping weight 4
lbs. (2 kg).

Ultraviolet Lamp Kits

Includes a 254 nm UV lamp and ballast.
Note: Not recommended for Purifier Cell Logic Biosafety Cabinets with
Scope-Ready Package.

Catalog # For use with Shipping Weight

3858500 All 115 VAC models 5 lbs. (2.3 kg)
3858501 All 100 VAC models 5 lbs. (2.3 kg)
3858502 All 230 VAC models 5 lbs. (2.3 kg)

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Appendix D: Accessories

IV Bar Kits

Bar supports intravenous solution bottles and bags. Kits include IV bar,
mounting hardware, and four hangers.

Catalog # For use with Shipping Weight

3858600 3-foot Purifier Cell Logic 3 lbs. (1.4 kg)
3858601 4-foot Purifier Cell Logic 4 lbs. ( 1.8 kg)
3858602 5-foot Purifier Cell Logic 5 lbs. (2.3 kg)
3858603 6-foot Purifier Cell Logic 6 lbs. (2.7 kg)

Shelf (# 3859000)

The wire shelf measures 8 inches wide by 5 inches deep, with a weight
capacity of 2.2 lbs. (1 kg) and mounts to the rear grille. Shipping weight 5.0
lbs. (2.3 kg).

Cord/Tubing Management System (# 3859100)

The kit includes three cord hooks and carabineers. The hooks mount to the
rear grille, and the carabineers secure power cords and/or tubing along the
rear wall of the work area. Shipping weight 5.0 lbs. (2.3 kg).

Prefilter

The prefilter is a sponge-type, adhesive-backed element that attaches to the
towel catch, to prevent objects from being drawn into the blower or onto the
HEPA filters.

Catalog # For use with Shipping Weight

3859200 3-foot Purifier Cell Logic 1 lbs. (0.5 kg)
3859201 4-foot Purifier Cell Logic 1 lbs. (0.5 kg)
3859202 5-foot Purifier Cell Logic 1 lbs. (0.5 kg)
3859203 6-foot Purifier Cell Logic 1 lbs. (0.5 kg)

Postfilter

The postfilter is a carbon-impregnated, cardboard-framed disposable filter.
These filters require either a factory-installed postfilter kit, or installation of a
Postfilter Conversion Kit, shown below.

Catalog # For use with Shipping Weight

3837201 4-foot Purifier Cell Logic 5 lbs. (2.3 kg)
3837202 5-foot Purifier Cell Logic 7 lbs. (3.2 kg)
3837203 6-foot Purifier Cell Logic 10 lbs. (4.6 kg)

Not available for 3-foot models

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Appendix D: Accessories

*Postfilter Conversion Kit

These kits allow the retrofitting of carbon postfilters in Logic cabinets that
originally did not have this option.

Catalog # For use with Shipping Weight

3859301 4-foot Purifier Cell Logic 5 lbs. (2.3 kg)
3859302 5-foot Purifier Cell Logic 5 lbs. (2.3 kg)
3859303 6-foot Purifier Cell Logic 5 lbs. (2.3 kg)

*Canopy Connection Kit

Contains the transition for thimble ducting the Biosafety Cabinet Logic
Series Cabinets to a 10-inch diameter stainless steel duct.

Catalog # For use with Duct Dia. Shipping Weight

3898000 3-foot Purifier Cell Logic 10" 15 lbs. (7 kg)
3898001 4-foot Purifier Cell Logic 10" 15 lbs. (7 kg)
3898002 5-foot Purifier Cell Logic 10" 15 lbs. (7 kg)
3898003 6-foot Purifier Cell Logic 10" 15 lbs. (7 kg)

*Air-Tight Damper (# 3776800)

The 10-inch diameter stainless steel damper mounts atop the Canopy
Connection Kit to adjust exhaust airflow. Shipping weight 13.0 lbs. (5.9 kg).

NOTE: This damper IS NOT prepared for the installation of the canopy
alarm sensor. If you want a canopy airflow sensor, order #3859700, listed
below.

*Backdraft Damper (# 3858800)

The backdraft damper mounts in a vertical run of duct to prevent the reverse
pressurization of the exhaust system. Sized for 10-inch diameter ductwork.
Shipping weight 5.0 lbs. (2.3 kg).

*Remote Blowers

Labconco offers a full line of epoxy-coated steel remote exhaust blowers for
use with its Biosafety Cabinets. Please contact Labconco’s Customer Service
Department at 800-821-5525 for assistance with blower selection and sizing.

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Appendix D: Accessories

Ergonomic Chair with Armrests (# 3744000)

Lab chair has 6-way articulating seat and back control for personalized
adjustment. Pneumatic mechanism adjusts seat height from 18 1/4" to 25
3/4". Five-leg black reinforced composite base rests on 2" ball bearing
casters. Aluminum support ring. Removable arm rests. Black vinyl
upholstery. Shipping weight 35 lbs. (15.9 kg).

Adjustable Footrest (# 3746000)

Elevates feet and permits angle repositioning while in use. 18 1/2"w x 11
1/2"d x 8"h. Shipping weight 6 lbs. (2.7 kg).

*Inflow/Downflow Sensor Kit (#3859400)

Includes airflow sensor, hardware and wiring needed to display inflow and
downflow on the Information Center LCD. Shipping weight 3 lbs. (1.4 kg).

Aspirator Pump Kit (# 3850300 or 3850301)

Kit includes components required to outfit the cabinet with a system to safely
aspirate fluids. The pump has an integrated 2L glass jar with fill limiter that
seals to contain fluids. The chemically resistant diaphragm pump can be
switched with the foot pedal provided. 10-ft. of clear tubing, filter as well as
a sealed grommet for the cabinet wall is in the kit.

• 3850300 Vacuum Aspiration Kit (Logic) 110V

• 3850301 Vacuum Aspiration Kit (Logic) 220V

Turntable (# 3852000)

The 12-inch diameter Turntable is intended to be utilized in the rear corners
of the biosafety cabinet work area to provide quick access to supplies. Ball
bearing hub and stainless steel upper and lower surfaces are autoclaveable.

*ULPA Supply Filters (# 3859500, 01, 02, 03)

Rated at 99.999% efficiency with particles 0.12 microns.

*ULPA Exhaust Filters (# 3859600, 01, 02, 03)

Rated at 99.999% efficiency with particles 0.12 microns.

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Appendix D: Accessories

Pure-Vu Air Barrier Seals

Used to seal microscopes on Cell Logic Biosafety Cabinets with Scope­Ready package. Note: Stereo microscope’s eyepieces have a broad range of
eyepiece height movement and will usually require the pleated Pure-Vu XL
Seal sizes, 4027810 and 4027811. Contact Labconco for ordering
information on Pure-Vu Seals for microscopes not listed.

Labconco P/N Pure-Vu Seal Size Nominal A2 Eyepiece Height Fits Microscope

4027800 00 Blank, must be punched

4027801 0.5" (12.7 mm)

Diameter Punch Kit

4027810 10 Universal XL,

4027811 11 XL Stereo 16"

4027812 12 12.0"

4027813 13 13.0"

4027814 14 14.0" Zeiss Axiovert 40

4027815 15 15.0" Nikon Eclipse TS 100

4027816 16 16.0" Leica DM IL LED

4027817 17 17.0" Leica DM 6000 B Digital

Used with 4027800
and 4027810

Oversized Blank
(+/- 2.0" height adjustment)

(+/- 2.0" height adjustment)

All, ships with cabinet

Ships with cabinet

All stereoscopes, Zeiss
Discovery V8 Stereo

Nikon SMZ 1500 Stereo,
Olympus SZX 16 Stereo,
Leica MZ95 Stereo

Inverted

Inverted, Olympus
CKX31 Inverted

Inverted

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Appendix D: Accessories

Stand-Still Isolation Platforms

Used to support microscopes, isolate vibration, and promote airflow
containment.

4028200 Stand-Still Isolation Platform (Std. Size 12" (305 mm) wide x

18.75" (476 mm) deep x 1.5" (38 mm) high.

4028100 Stand-Still XL Isolation Platform (Extended Size 12" (305 mm)
wide x 22.75" (578 mm) deep x 1.5" (38 mm) high. Typically used with
stereo microscopes requiring forward extended support.

Tube Racks/Blocks/Holders, Circulator Shelf,
Connectors and Tubing

Used to hold samples for Temp-Zone and connect the user-supplied
circulator.

Labconco P/N Tube Size/Sample Volume Block Tube

Capacity
4026400 0.5 ml microcentrifuge, 7.5 mm 18 2 x 9
4026401 0.5 ml microcentrifuge, 7.5 mm 96 8 x 12
4026402 1.5 ml microcentrifuge, 10.5 mm 18 2 x 9
4026403 1.5 ml microcentrifuge, 10.5 mm 96 8 x 12
4026404 1.5 ml, 10 mm 12 2 x 6
4026405 5 ml, 12 mm 12 2 x 6
4026406 6 ml, 13 mm 12 2 x 6
4026407 15 ml round, 16 mm 12 2 x 6
4026408 15 ml conical 12 2 x 6
4026409 50 ml round, 28 mm 4 4 x 1
4026410 50 ml conical, 28 mm 4 4 x 1
4026411 Microtiter Well Plates, 3.4" x 5.0"

1.5 ml microcentrifuge

0.5 ml microcentrifuge
4026600 Universal Bead Bath, Small (0.25l) Universal 4 x 2
4026601 Universal Bead Bath, Large (0.5l) Universal 4 x 4
4026602 Beads for Universal Bead Bath,

0.75 liter volume
4028600 Shelf, Small Circulator (fits

Hydraulic Lift Base Stands)
1543400 Polyethylene Tubing 3/8" (order by ft.)
9108000 Tube Support Insert for 3/8"

Polyethylene Tubing
1621000 Foam Rubber Insulation
1548900 Plug-in Elbow 3/8
1544603 Male Connector 3/8 Tube x 1/8 NPT
1544600 Male Connector 3/8 Tube x 1/4 NPT
1544605 Male Connector 3/8 Tube x 3/8 NPT

Well plate

12
12

Arrangement

1 x 1
1 x 12
1 x 12

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69

AAppppeennddiixx EE::
QQuuiicckk CChhaarrtt

Model 34309 34409 34509 34609

Cabinet Size (in feet) 3 4 5 6

Sash Opening (inches) 9 9 9 9

Starting Serial #

Nominal Avg. Downflow (FPM) 55+/-5 55+/-5 55+/-5 55+/-5
Nominal Average Inflow (FPM) 105+/-5 105+/-5 105+/-5 105+/-5

Supply HEPA Data

Labconco P/N 3838400 3838401 3838402 3838403

Exhaust HEPA Data

Labconco P/N 3838500 3838501 3838502 3838503

Motor/Blower Data

Labconco P/N2 3832200 3832201 3832202 3832203

Motor HP 1/2 1/2 3/4 3/4

Blower Size (inches) 10 x 8 10 x 8 10 x 8 10 x 8

Fluorescent/UV Lamp Data

Fluorescent Lamps (2 each) F25T8 TL741 F32T8 TL741 F40T8 SP41 F40T8 SP41

Light intensity (ft-candles)3 90-100 90-100 90-100 90-100

UV Lamp G30T8 G30T8 G30T8 G30T8

UV energy(uW-cm2)4 240 240 240 240

Prefilter Data

Labconco P/N 3858900 3858901 3858902 3858903

Postfilter Data

Labconco P/N NA 3837201 3837202 3837203

Canopy Data

Avg. Exhaust Vol. Range (CFM) 228-251 304-333 378-416 453-498

Canopy Alarm Setpoint Vol. (CFM)5

Nominal Canopy Vol. (CFM) 323 401 480 590

Canopy Vol. Range (CFM) 311-335 385-417 458-502 569-611

Canopy Slot Area (F2) 0.135 0.186 0.236 0.304
Nominal Slot Velocity (FPM) 550 400 315 330
Nominal Slot Volume (CFM) 75 75 75 100

Canopy Vacuum (In. H2O) .15-.25 .15-.25 .15-.25 .15-.25

Slot Velocity Alarm Setpoint (FPM) 150 100 100 100

Labconco Canopy P/N 3898000 3898001 3898002 3898003

1

0705_ 0705_ 0705_ 0705_

228-251 304-333 378-416 453-498

1. The primary serial tag is on the lower outside edge of the right corner post.

The secondary serial tag is located on the front of the electronics module on
the top right side of the cabinet. The first two digits of the serial number are
the year of production, the next two are the month. The next 5 digits are the
sequence of production, and the letter following the serial number is the
revision level of the cabinet.

2. Each motor must be programmed by Labconco for the appropriate width

cabinet.

3. As per ANSI/NSF Std. 49 with a background of 15 ft-candles.

4. Measured in the geometric center of the work surface.

5. If the optional Canopy Alarm is installed

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AAppppeennddiixx FF::
IInnssttaallllaattiioonn GGuuiiddee ffoorr aallll PPuurree--VVuu

allss aanndd SSttaanndd--SSttiillll IIssoollaattiioonn

SSeea
PPllaattffoorrm

Figure F-1 is an aid to show the proper installation of the pure-Vu Seal for
the Cell Logic Biosafety Cabinet with Scope-Ready Package. Figure F-1
also shows the proper placement of the Stand-Still Isolation Platform.

mss

Figure F-1

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

Figure F-2 is an aid to show the Pure-Vu Seal fully installed with the
eyepieces extended and sealed. Figure F-2 also shows the microscope moved
forward into proper position on the Stand-Still Isolation Platform.

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Figure F-2

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

Stereoscopes with forward extending bases and frequent eyepiece height
adjustment typically require the selection of a Pure-Vu XL Seal and Stand­Still XL Isolation Platform. Figures F-3 through F-10 are an aid in setting up
the pleated Pure-Vu XL Seal. Figures F-11 and F-12 show typical complete
installations of stereo microscopes and proper placement of the Stand-Still
XL Isolation Platform. Listed next are instructions for setting up the pleated
design of the Pure-Vu XL Seal.

1. Per Figure F-3, with the outer aerodynamic collar removed, place the
Pure-Vu XL Seal over the inner aerodynamic mounting collar.
Locate the top five holes and secure with transparent tape as shown.
Pure-Vu XL Seals allow for eyepiece height flexibility of +/- 2.0" (51
mm).

Figure F-3

2. Per Figure F-4, form the first pleat and secure with transparent tape.
The seal shown has pre-cut eyepiece holes (Labconco #4027811) and
is designed for the upper portion of the glass cutout. This upper
portion of the glass cutout is used frequently with stereoscopes.
However, should you need a pleat at the middle or lower position of
the glass cutout, then the pleat can be moved lower when setting up
the Blank Pure-Vu XL Seal (4027810). See Figure F-4.

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Figure F-4

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

3. Per Figure F-5, form the second pleat and secure with transparent
tape.

Figure F-5

4. Per Figure F-6 and Figure 3-5, place the Outer Aerodynamic collar
over the Pure-Vu XL Seal and lightly secure in place with the 14
Phillips mounting screws. Do not tighten.

Figure F-6

5. Per Figure F-7, remove the transparent tape and then tighten the 14
Phillips mounting screws.

Figure F-7

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

6. Per Figure F-8, move the microscope forward through the holes in the
Pure-Vu Seal and install the eyepiece. A tight seal should be formed
around the eyepiece.

Figure F-8

7. Figure F-9 shows the finished installation.

Figure F-9

8. Per Figure F-10, adjust the scope eyepiece height for proper focus for
the specimen range.

Figure F-10

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

9. Figure F-11 shows the completed installation of the Pure-Vu XL Seal
and Stand-Still XL Isolation Platform for one scope manufacturer.
The Pure-Vu XL Seals extend 5.5" below the aerodynamic collar.

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Figure F-11

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

10. Figure F-12 shows a different installation of the Pure-Vu XL Seal and
Stand Still XL Isolation Platform for another separate scope
manufacturer.

Figure F-12

11. Refer to Appendix D: Accessories to order Pure-Vu Seals and Stand
Still Isolation Platforms and use Chapter 3 and Appendix F as an aid
for proper setup.

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

Pure-Vu Template

Note: Not to scale, see next page.

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Appendix F: Installation Guide Pure-Vu Seals/Stand-Still Isolation Platforms

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79

DECLARATION OF CONFORMITY

Application Council Directive(s): 73/23/EEC, 89/336/EEC, 2002/95/EC (RoHS),

2004/108/EC

Standard(s) to which conformity is declared: EN61010-1, EN55022, EN61000-3-2/3,
EN61326-1

Manufacturer’s Name: Labconco Corporation

Manufacturer’s Address: 8811 Prospect Avenue
Kansas City, MO 64132 USA

Importer’s Name: See Shipping/Customs Documents

Importer’s Address: See Shipping/Customs Documents for your equipment

Type of Equipment: Laboratory Equipment

Model No.: Purifier Cell Logic Series Biological Safety Cabinets

34309-XX 3' Wide, 9" Sash
34409-XX 4' Wide, 9" Sash
34509-XX 5' Wide, 9" Sash
34609-XX 6' Wide, 9" Sash
34410-XX 4' Wide, 8" Sash, Total Exhaust
34610-XX 6' Wide, 8" Sash, Total Exhaust

Serial No.: Various – See Individual Declaration

Year of Manufacture: 2009 and Subsequent

I, the undersigned, hereby declare that the equipment specified above conforms to the
above Directive(s) and Standard(s).

See individual Declaration of Conformity which
will be signed by the importer for your country.

Place: _______________________________________
(Signature)

Date: _______________________________________
(Full Name)

_______________________________________

(Position)

Labconco P/N 36960-71, Rev. A, F676

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