IRIS Cytofuge 12 Operator's Manual

Cytofuge®12 Cytocentrifuge System

A Division of IRIS International, Inc.

Operator’s Manual

Cytofuge®12 Cytocentrifuge System

Model Number M802

For in-vitro diagnostic use

Product Number

CF12 – Cytofuge 12 for 100-240 VAC, 50/60 Hz

StatSpin and Iris are registered trademarks of IRIS International, Inc.

Printed in U.S.A.

Table of Contents

How to use this manual ...................................................................................... ii

Section 1 ............................................................................................................. 1

Inspect Packaging .......................................................................................... 1

Verify Contents ............................................................................................... 1

Install .............................................................................................................. 1

Connect Power ............................................................................................... 2

Section 2 ............................................................................................................. 3

Principle and Intended Use ............................................................................ 3

Symbols and Definitions ................................................................................. 3

Operator Controls ........................................................................................... 4

Error Indicators ............................................................................................... 5

Accessories .................................................................................................... 5

Section 3 ............................................................................................................. 6

Overview ........................................................................................................ 6

Sample Volumes ............................................................................................ 6

Pre-concentration ........................................................................................... 6

Dilution ........................................................................................................... 6

Section 4 ............................................................................................................. 7

Opening and Closing the Cover ..................................................................... 7

Using the Removable Rotor ........................................................................... 7

Spinning Samples........................................................................................... 8

Programming .................................................................................................. 9

Section 5 ........................................................................................................... 10

Overview ...................................................................................................... 10

Cleaning ....................................................................................................... 10

Checking/Replacing Rotor Seal .................................................................... 11

Checking/Replacing Drive Cone O-rings ...................................................... 11

Checking the Rotor Speed ........................................................................... 12

Troubleshooting ............................................................................................ 12

Service ......................................................................................................... 13

Section 6 ........................................................................................................... 14

Specifications ............................................................................................... 14

References ................................ ................................ ................................ ... 15

Iris Sample Processing Warranty ................................................................. 16

Limitation of Liability ..................................................................................... 16

How to use this manual

WARNING - Do not expose the cytocentrifuge or its rotor to strong or concentrated acids, bases or oxidizing agents, aromatic or halogenated hydrocarbons, esters or ketones.

WARNING - Do not use the supplied line cord outside of North America. Use a power cord with a grounded male connector suitable for the power outlet being used, which is rated at 3.0 Amps or more and has an IEC320/CEE22 female connector to attach to the cytocentrifuge.

WARNING - Maintain a 300 mm (11.8 inches) clearance envelope around cytocentrifuge during operation. Do not deposit any potentially hazardous materials within the CLEARANCE ENVELOPE.

WARNING - Inspect instrument for cracks or any physical damage to housing, cover, lid and rotor. Damage may result in unsafe operation; discontinue use until repairs have been performed.

CAUTION - Do not overfill the concentrators. Addition of excess amounts of liquid to Cell or Filter

Concentrators will result in the fluid being “spun out” during centrifugation.

CAUTION - Do not place the cytocentrifuge on top of other equipment and do not place other objects or equipment on top of the cytocentrifuge.

CAUTION - The cover interlock by-pass is for emergency use only. Do not attempt to operate the Cytofuge 12 while the latch release tool is inserted in the housing. Misuse of the interlock bypass may expose the operator to unsafe conditions.

CAUTION - Before using any cleaning or decontamination methods except those recommended, operators should check with customer service that the proposed method will not damage the equipment.

CAUTION - The rotor (CFRT-12) is designed for many years of service but, depending on the frequency of use, the rotor components may develop cracks over time or if dropped on a hard surface. Inspect the rotor for damage or cracks before each use. If damage is found discontinue use and contact customer service or your distributor for a replacement.

CAUTION - Do not lift the rotor (CFRT-12) by the locking button - the lid may release allowing the rotor bowl to fall. Always lift the rotor by the black center grip.

CAUTION - The rotor (CFRT-12) is intended for use with biological samples only. Do Not place flammable or reactive substances in the rotor.

CAUTION - While in the resting position, the rotor is designed to maintain the Concentrators at an angle to prevent fluid from contacting the slide and/or the filter card prior to spinning. Keep the rotor level during transport to and from the cytocentrifuge to ensure proper concentrator angle.

CAUTION - Do not defeat any safety interlocks while checking the rotor speed. Bypassing interlocks could result in personal injury.

CAUTION - Unplug the cytocentrifuge from the wall outlet before cleaning or performing maintenance.

This manual along with information contained on product labels and in package inserts should provide you with all the information you need to operate and maintain the Cytofuge 12 Cytocentrifuge system.

When the information requires special attention, a caution symbol appears next to the italicized text.

Please pay close attention to the instructions that accompany the notes and symbols as well as the standard laboratory practices outlined by your facility and local regulatory agencies. The table below lists all the CAUTIONS and WARNINGS for the Cytofuge® 12 Cytocentrifuge.

iii

CAUTION – Do not lean, or place materials, on the cytocentrifuge.

CAUTION - Follow Universal Precautions with all biological specimens, regardless of whether the

specimen is known to contain an infectious agent. Bioseals cannot be relied upon as a sole safeguard against contamination by pathogens. Always load and unload the rotor under a biological safety hood. (See references).

CAUTION – The seals in the removable rotor are intended to stop gross fluid leakage only. These seals should not be relied upon for protection against biohazardous contamination.

Please use the system as intended. Improper use of the Cytofuge® 12 Cytocentrifuge and/or its accessories may cause personal injury, damage to the system, inaccurate results, or potentially nullify warranties.

The Cytofuge® 12 Cytocentrifuge System, and its associated components and consumables are covered under U.S. Patent Nos. 5,480,484 & 5,679,154.

Inspect Packaging

The package contains:

One Cytofuge® 12 Cytocentrifuge (CF12)

One cytocentrifuge rotor (CFRT-12)

One Accesory Carton The Accesory Carton consists of:

One line cord (for use in North America only).

One Operator’s Manual CD

One latch release tool

3-well Cell Concentrators and 3-well gaskets pkg of 4, (CC03)

1-well Cell Concentrators and 1-well gaskets pkg of 4, (CC01)

Stainless Steel Clips for Cell Concentrators pkg of 8, (CLIP)

Backing Plates for Cell Concentrators pkg of 4, (BP01)

Filter Concentrators 2 bags of 4 each (FF01)

Stainless Steel Clips for Filter Concentrators 3 pkgs of 4, (FFCL)

The Cytofuge accessories have been damaged in transport, please inform your carrier immediately.

NOTE: Save the shipping carton and packing materials to facilitate return should service be required.

12 Cytocentrifuge and its accessories are delivered in a single carton. If the cytocentrifuge or

Verify Contents

Section 1

Unpacking and Installation

Install

o Place the cytocentrifuge on a solid level surface suitable for operation of laboratory instrumentation.

CAUTION - Do not place the cytocentrifuge on top of other equipment and do not place other objects or equipment on top of the cytocentrifuge.

o Position the cytocentrifuge out of direct sunlight and away from sources of intense heat or cold.

Improper conditions can cause damage to the housing, rendering unit unsafe to operate.

o See the Appendix for the acceptable range of operating temperature and humidity. o Remove the foam shipping retainer around the rotor before operating the cytocentrifuge.

WARNING - Maintain a 300 mm (11.8 inches) clearance envelope around cytocentrifuge during operation. Do not deposit any potentially hazardous materials within the CLEARANCE ENVELOPE.

Connect Power

o Plug the cytocentrifuge into a grounded outlet supplying 100-240 V @ 50-60 Hz. o Turn on the power switch (located on the rear panel, above the line cord connector). o When the Cytofuge 12 completes the power-on check it will give a two-beep ready signal. Press the

STOP/OPEN button to release the latch and open cover.

+ 14 hidden pages

IRIS Cytofuge 12 Operator's Manual

Specifications and Main Features

Frequently Asked Questions

User Manual