VIAFLO Electronic Pipettes Operating instructions
900-00002-00_V05
Declaration of conformity | Konformitätserklärung |
Déclaration de conformité | Declaración de conformidad |
Dichiarazione di conformità
INTEGRA Biosciences – 2 Wentworth Drive, Hudson, New Hampshire 03051
declares on its own responsibility that the product | erklärt in alleiniger Verantwortung,
dass das Produkt | déclare sous sa responsabilité exclusive, que le produit |
declara bajo su propia responsabilidad que el producto | dichiara sotto la propria
responsabilità che il prodotto
VIAFLO Electronic
Pipettes
in accordance with EC directives | gemäss der EU-Richtlinien | est conforme au terme
directives CE | de acuerdo con las directivas CE | in conformità alle direttive CE
2006/95/EC Low voltage equipment
2004/108/EC Electromagnetic compatibility
2011/65/EC Restriction of Hazardous Substances
2002/96/EC Waste Electrical and Electronic Equipment
is in compliance with the following normative documents: | mit den folgenden normativen
Dokumenten übereinstimmt: | aux documents normatifs ci-après: | cumple las
documentos normativos: | soddisfa le normative seguenti:
EN 61010-1 Safety requirements for electrical equipment for measurement,
EN 61326-1 Electrical equipment for measurement, control and laboratory
Standards for Canada and USA
CAN/CSA-C22.2
No. 61010-1
UL Std. No. 61010-1 Safety requirements for electrical equipment for measurement,
FCC, Part 15, Class A Emission
Models: 4011, 4012, 4013, 4014, 4015, 4021, 4022, 4023,
4024, 4031, 4032, 4033, 4034, 4041, 4042, 4121, 4122, 4123,
4124, 4131, 4132, 4143, 4144, 4163, 4164
control and laboratory use - General requirements.
use - EMC requirements.
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
control and laboratory use - General requirements.
Hudson, New Hampshire, USA - April 5, 2013
Gary Nelson
President
George Kalmakis
VP Operations
VIAFLO Electronic Pipettes – Operating instructions V05
Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................7
1.2 Intended use.....................................................................................7
1.3 Safety notes .....................................................................................8
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................9
2.2 Pipette configurations.......................................................................9
2.3 Overview of the VIAFLO Electronic Pipettes..................................10
2.3.1 VIAFLO pipette parts ..........................................................10
2.3.2 Back view............................................................................11
2.3.3 Display ................................................................................11
2.3.4 Touch wheel........................................................................12
2.3.5 Left and right arrow buttons ................................................12
2.3.6 Purge button .......................................................................12
2.3.7 Run key...............................................................................12
2.3.8 Tip ejector ...........................................................................13
2.3.9 Reset button........................................................................13
Chapter 3 Installation
3.1 Operating environment ...................................................................14
3.2 Charging the battery.......................................................................14
3.2.1 Charging the battery on a stand..........................................14
3.2.2 Charging the battery with UPS............................................15
3.3 Toolbox - adapt your VIAFLO Electronic Pipettes..........................15
3.3.1 Preferences.........................................................................16
3.3.2 Calibration & Services.........................................................17
3.3.3 Communications .................................................................18
3.3.4 Owner Information ..............................................................18
3.3.5 Help Language....................................................................19
3.3.6 Write protect........................................................................19
3.3.7 Password key......................................................................19
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VIAFLO Electronic Pipettes – Operating instructions V05
Chapter 4 Operation
4.1 Turn on/off the device.....................................................................20
4.2 Attaching and removing GripTips ...................................................20
4.3 Start pipetting .................................................................................21
4.3.1 Pipetting..............................................................................21
4.3.2 Blowout modes ...................................................................21
4.3.3 Recommendations for pipetting ..........................................22
4.4 Pipetting options and settings ........................................................23
4.4.1 Edit option...........................................................................23
4.4.2 Volume selection.................................................................23
4.4.3 Speed selection ..................................................................24
4.4.4 Pace....................................................................................25
4.4.5 Count, Mix Cycle and Rows................................................25
4.4.6 Help.....................................................................................25
4.5 VOYAGER tip spacing ...................................................................26
4.5.1 Set the number of tip positions and the tip spacing ............26
4.5.2 Tip spacing operation..........................................................27
4.5.3 Homing the tips...................................................................27
4.6 Troubleshooting/FAQ .....................................................................28
4.6.1 General ...............................................................................28
4.6.2 Electronic ............................................................................29
Chapter 5 Pipetting modes
5.1 Overview pipetting modes ..............................................................31
5.2 Detailed description of pipetting modes .........................................32
5.2.1 Pipet mode..........................................................................32
5.2.2 Repeat dispense mode .......................................................33
5.2.3 Sample dilute mode ............................................................34
5.2.4 Pipet/mix mode ...................................................................34
5.2.5 Manual pipet mode .............................................................35
5.2.6 Reverse pipet mode............................................................36
5.2.7 Variable dispense mode .....................................................37
5.2.8 Variable aspirate mode .......................................................38
5.2.9 Sample dilute/mix mode......................................................39
5.2.10 Serial dilution mode ..........................................................40
5.3 Custom step-based programming mode ........................................41
5.3.1 Create a custom program ...................................................41
5.3.2 Program tip adjustments in a custom program ...................42
5.3.3 Modify existing programs ....................................................42
5.3.4 Example of custom mode ...................................................43
4 INTEGRA Biosciences AG
VIAFLO Electronic Pipettes – Operating instructions V05
Chapter 6 Maintenance
6.1 Cleaning .........................................................................................44
6.1.1 Assembly of single channel pipettes...................................44
6.1.2 Assembly of multichannel fixed spacing pipettes................46
6.2 Sterilization.....................................................................................48
6.2.1 Autoclaving the disassembled components ........................ 49
6.3 Servicing.........................................................................................49
6.3.1 Shipping to INTEGRA Biosciences.....................................49
6.3.2 Changing O-rings of tip fittings............................................49
6.3.3 Lubrication ..........................................................................50
6.4 Calibration ......................................................................................51
6.4.1 Materials .............................................................................51
6.4.2 Definitions ...........................................................................51
6.4.3 Test Conditions and Environment.......................................52
6.4.4 Pre-wetting of tips ...............................................................52
6.4.5 Leak test .............................................................................53
6.4.6 Obtaining the Actual Volume ..............................................54
6.4.7 Calculation of accuracy and precision ................................55
6.4.8 Adjusting VIAFLO Electronic Pipettes.................................56
6.5 Equipment disposal ........................................................................58
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................59
7.2 Specification of the device..............................................................59
7.3 Pipetting speed...............................................................................59
7.4 Pipette Specifications .....................................................................60
7.5 Z Correction Factors.......................................................................63
Chapter 8 Accessories
8.1 Accessories ....................................................................................64
8.2 Consumables .................................................................................64
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VIAFLO Pipettes – Operating instructions V05
Imprint
© 2013 INTEGRA Biosciences AG
All rights to this documentation are reserved. In particular the rights of reproduction,
processing, translation and the form of presentation lie with INTEGRA Biosciences AG.
Neither the complete documentation nor parts thereof may be reproduced in any way, or
stored and processed using electronic media or distributed in any other way without the
written consent of INTEGRA Biosciences AG.
This operating instruction manual has part number 900-00002-00, the version is V05. It
applies to firmware version 2.44 or higher until a newer revision is released.
VIAFLO, VOYAGER, VIALINK, and GripTip are trademarks of INTEGRA Biosciences,
Hudson, NH.
Manufacturer
INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland
T +41 81 286 95 30
F +41 81 286 95 33
info@integra-biosciences.com
www.integra-biosciences.com
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA
T +1 603 578 5800
F +1 603 577 5529
Customer service
Please contact your local INTEGRA Biosciences representative.
To find out name and address of your local representative go to
www.integra-biosciences.com
Further information and operating instructions in other languages are available on
www.integra-biosciences.com
6 INTEGRA Biosciences AG
.
or on request info@integra-biosciences.com.
VIAFLO Pipettes – Operating instructions V05 Introduction
1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the VIAFLO Electronic Pipettes. This chapter informs about the
symbols used in these operating instructions, the intended use of the VIAFLO Electronic
Pipettes and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
ARNING
W
This safety symbol warns against hazards that could result in injury. Italso
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
OTE
N
This symbol identifies important notes regardi ng the correct operation of the
device and labour-saving features.
1.2 Intended use
VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven
pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5 5000 µl using GripTip pipette tips.
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VIAFLO Pipettes – Operating instructions V05 Introduction
1.3 Safety notes
VIAFLO Electronic Pipettes comply to the recognized safety regulations and is safe to
operate. VIAFLO Electronic Pipettes can only be operated when in perfect condition and
while observing these operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the VIAFLO Electronic Pipettes must have
read and understood these operating instructions, and particularly, the safety notes, or
must have been instructed by supervisors so that safe operation of the device is
guaranteed.
Use only an INTEGRA battery.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Do not open or modify the VIAFLO Electronic Pipettes in any way. Repairs may only be
performed by INTEGRA Biosciences AG or by an authorised after-sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
ARNING
W
Do not use the VIAFLO Electronic Pipettes near flammable material or in
explosive areas. Also, do not pipette highly flammable liquids such as
acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
Do not immerse the VIAFLO Electronic Pipettes in liquid. The fluid can
damage internal parts. Avoid pipetting of liquids whose vapors could attack
the materials PA (polyamide), POM (polyoxymethylene), FPM (fluor -rubber),
NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive vapors could also
damage metallic parts inside the device.
OTE
N
Prolonged exposure of the VIAFLO Electronic Pipettes to UV-light can cause
discolouration and/or yellowing of the control unit. However, this will not
affect the performance of the device in any way.
8 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Description of the device
2 Description of the device
2.1 Scope of delivery
• VIAFLO pipette
• Rechargeable battery (located inside the pipette, Li-ion, 3.7 V, 1050 mAh)
• Bag of spare O-rings (300 and 1250 µl versions only)
• Accuracy and precision certificate
AUTION
C
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is damaged,
instead contact your local dealer.
2.2 Pipette configurations
VIAFLO single and multichannel pipettes couple an easy-to-use touch wheel controller
with intuitive programming. They are microprocessor controlled and stepper motor driven.
In addition, the VIAFLO VOYAGER adjustable tip spacing pipettes provides you with the
ability to vary tip spacing.
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VIAFLO Pipettes – Operating instructions V05 Description of the device
1
2
5
6
7
8
3
4
9
11
12
10
2.3 Overview of the VIAFLO Electronic Pipettes
2.3.1 VIAFLO pipette parts
1 Display
2 Back button, to navigate backward
3 Touch wheel, spin to scroll and move the cursor
4 OK button, to make a selection
5 Left and right arrow buttons, for selections
6 Purge button, to empty tips
7 Run key, to start operations
8 Tip ejector
9 Finger hook, facilitates easy operation
10 Volume indicator label, color matches GripTip rack
insert.
11 Ejector sleeve
12 Tri-lobe tip interface
10 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Description of the device
13
14
15
16
RUN
Current mode
Active step
OK
2.3.2 Back view
13 Reset button
14 Power connector
15 Charging stand interface
16 Battery
2.3.3 Display
The Display shows all pipetting options.
Battery life indicator
Instruction
Vol umes
Options
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VIAFLO Pipettes – Operating instructions V05 Description of the device
2.3.4 Touch wheel
The Touch wheel is fully operational with only one hand. Rotational finger movements
translate into up and down cursor movement on the display. The Touch wheel is fully
functional with the use of latex gloves.
Move finger on the Touch wheel to choose (and
highlight) an option on the display. Press OK (4
make the selection.
When a setting dial is displayed, spin the Touch wheel
to change the value and press OK.
2.3.5 Left and right arrow buttons
At times, you will see and on a display screen. These buttons
are used to select options.
Press to select the option indicated with the left arrow (HELP, in
the example beside). Press to select the option indicated with the
right arrow (RUN, in the example).
For Voyager models, these buttons are also used for tip spacing adjustments.
2.3.6 Purge button
During pipetting, you can interrupt the current pipetting protocol and purge all remaining
liquid currently in the GripTips. To do so, press Purge (6
).
) to
The pipette will display a prompt:
To proceed, press and release the Run key (7
of the dispense, the first step in the current program will be
displayed.
2.3.7 Run key
Press and release the Run key to initiate aspiration, dispense, mix, purge, and special
pipetting operations. This button is centrally located for left- or right-handed actuation.
During dispense, you can press and hold the Run key to perform a two-step blowout, see
“4.3.2 Blowout modes” on page 21.
12 INTEGRA Biosciences AG
). Upon completion
VIAFLO Pipettes – Operating instructions V05 Description of the device
2.3.8 Tip ejector
The tip ejector easily ejects tips from the tip cones.
The serial number can be found beneath the tip ejector. Press and
hold the tip ejector in the down position to see the 7-digit serial
number.
2.3.9 Reset button
The reset button (13
RAM in the pipette. The programs stored in memory are maintained. Once pressed, the
VIAFLO startup screen will be displayed.
Press any key to continue and allow the instrument to initialize and home the pipette. The
routine ends by displaying the Main Menu.
) is located on the back of the VIAFLO pipettes. It is used to reset the
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VIAFLO Pipettes – Operating instructions V05 Installation
3 Installation
3.1 Operating environment
The VIAFLO Electronic Pipettes have been designed for use in a laboratory. They shall be
operated in a dry and dust-free location with a temperature of 5–40 °C and a maximal
(non-condensing) relative humidity of 80 %.
3.2 Charging the battery
All VIAFLO hand-held pipettes share the same rechargeable, long-life, lithium-ion battery
that has a capacity of 1050 mAmp/hours. The battery should be charged for at least one
hour before pipetting. A full charge takes 2.5 hours and will provide approximately 3500
pipetting cycles.
A battery indicator is provided on the pipette display (2.3.3
• Blinking red icon: Low battery. A recharging message will appear.
• Blinking grey icon: Pipette is recharging.
• Blinking green icon: Pipette is recharging and battery is 90% charged.
• Solid green icon: Battery is fully charged and the pipette is still connected to the mains
adapter.
• Three static grey bars: Battery is fully charged.
AUTION
C
Use only the approved INTEGRA battery, power supply or charging stand.
Use of an incompatible power transformer can damage the pipette.
Battery charging can take place in two ways, using a pipette charging stand or the
Universal Voltage Power Supply (UPS), that allows for pipetting while charging the battery,
see (“8.1
3.2.1 Charging the battery on a stand
Use one of the different charging stands - single position with two- (#4210) or four prong
connector (#4211), or four position with two prong connector (#4215) - to charge the
battery.
Accessories” on page 64).
Place the pipette on the charging stand by fitting the
power receptacle (15, on the top back of the pipette)
over the prong connector on the top of the stand.
Plug the cable of the adequate power supply to the
socket at the base of the stand (see “8.1
on page 64).
) indicating different states:
Accessories”
AUTION
C
Always use the correct power supply for the charging stand.
14 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Installation
The pipette will turn fully on when placed on the stand. When the dimming time is achieved
the startup screen is displayed on the pipette. When the turnoff time is reached, the pipette
turns off. For disconnecting simply lift the pipette up from the stand.
3.2.2 Charging the battery with UPS
With the optional Universal Voltage Power Supply (UPS, #4200), you can use the pipette
while charging through the line cord.
Insert the UPS connector into the receptacle on the top back of the
pipette (14
It will take approximately 30 seconds of charging to store enough
voltage to begin running the motor.
The pipette will turn fully on when the line cord is connected. The current screen remains
displayed. If the pipette is idle while charging, the display may dim (see “4.1 Turn on/off
the device” on page 20), but it will continue to show the battery life indicator. When the
display shut off time is reached, the instrument will shut off.
). Plug the UPS into a wall outlet.
3.3 Toolbox - adapt your VIAFLO Electronic Pipettes
The Toolbox provides options to adapt the device to appropriate applications, setting
personal preferences, calibration, computer connectivity and storing owner information.
Toolbox mode Description
Preferences Customizes the system parameters.
Calibration & Service Sets calibration and service history options.
Communications Enables communication between your VIAFLO Electronic
Pipettes and a PC.
Owner Information View your pipettor’s serial number and set a personal ID.
Help Language Sets language specific help.
Write Protect Protects programs or menu options from modification.
Password Key Displays the encrypted password.
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VIAFLO Pipettes – Operating instructions V05 Installation
3.3.1 Preferences
Preferences customizes your system parameters. Select a preference and press OK to
access.
Preference Description Range
Sound Simple tones indicate completion of operations and
errors. Select an option and press OK to change the
status of the beep tone between On and Off:
• Step complete: at the end of a program step
• Program complete: at program completion
• Purge key: when Purge is pressed.
• Error message: when an error message appears or
when illegal data entry is attempted.
• Spinner: When spinning the Touch w h e el.
• Last dispense: before the last dispense in Repeat
Dispense and Variable Dispense.
Display Customizes your display. Press OK to select an option,
use the Touch wheel to display the desired value.
• Start up display: Select the startup display at one of
the following: VIAFLO Logo, None or Custom (up to
two, uploaded with VIALINK). Press to Save your
selection.
• Display dim time: The display will dim after a set number of minutes. A shorter dim time helps preserve battery life. Press OK to save your selection.
• Turn off time: By default, the pipette will turn off after 5
minutes of idle time. A shorter turn off time helps preserve battery life. You can change this setting. Press
OK to save your selection.
Main Menu Select a function to be hidden from the main menu (Off)
and press OK, e.g. Tip Spacing, Pipet, Repeat Dispense,
Dilute, Pipet/Mix. On/Off
Spinner Adjust your Touch wheel spin sensitivity. Low, Medium,
Pipetting Select an option and press OK.
• Purge key speed: Choose the desired purge speed
and press OK to save your selection.
• Delayed blowin: Choose a timed delay between the
blowout and the blowin (delay before the piston
homes) at the end of a dispense, if no two step blowout
is performed, see “4.3.2 Blowout modes” on page 21
After changing desired settings, press to Save.
On/Off
VIAFLO Logo,
None,
Custom 1 or 2
Never,
1-20 min
5-20 min,
1 hour
High
1-10
0/0.5/1/1.5 s
.
16 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Installation
3.3.2 Calibration & Services
These options enable you to set calibration features and review service history .
Calibration
& Services
Calibration Allows for re-calibration of the VIAFLO Electronic
Calibration
Reminder
Time or
Cycles
Service History
Calibrate Tip
Spacing
After changing desired settings, press to Save.
Description Range
Pipettes to restore accuracy. The calibration factors for
Pipet and Repeat type are displayed.
To edit the calibration volumes, press .
• Target Volume: This is the volume you are interested
in using for the calibration.
• Actual Volume: This is the measured volume obtained
when dispensing the target volume.
• Factory Reset: Resets the correction factor back to
the original factory setting. Press to apply the factory
setting.
• Current Factor: Displays the factor currently in use.
Sets a calibration reminder based on a specified time
frame or number of pipetting cycles. When the calibration
reminder is displayed, press any key to confirm.
However, the reminder will reappear every time the
pipettor is turned on until you change the reminder time
or use the reset option.
• Timer: Press OK to turn the reminder timer On or Off.
• Remind in: Use the Touch wheel to set a reminder
interval for calibration (time in months or in thousands
of cycles).
• Reset: Resets the timer to the defined calibration interval. With this option highlighted, press OK and Save
to enable.
Displays notes of any service that took place on the
VIAFLO Electronic Pipettes listed newest entry first.
Starts calibration of tip spacing of VOYAGER pipettes. -
-
On/Off
1 -12 months
or 10k - 240k
cycles
-
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VIAFLO Pipettes – Operating instructions V05 Installation
3.3.3 Communications
The VIAFLO Electronic Pipettes can be programmed from a PC via a Single Pipette
Programming Stand (#4211) or via wireless Bluetooth connection.
VIALINK is a pipette management software for the PC and is used to manage VIAFLO
Electronic Pipettes. It can be downloaded from the INTEGRA website in the product
section and is free of charge for all VIAFLO Electronic Pipettes customers. A detailed
description of the software, along with the operating instructions, can be found on the
website as well.
Communications
Serial Turn serial communication mode “On” to begin bi-
Bluetooth Each pipette needs its own Blue Tooth chip. Blue Tooth
Press OK to toggle between On and Off.
3.3.4 Owner Information
Owner
Information
VIAFLO The first line is highlighted. Press to enter personal
In addition, information about your VIAFLO Electronic Pipettes, such as owner
identification, serial number (SN), pipette size, indication of single or multi channel, and
version number, are displayed automatically.
Description Range
On/Off
directional communication. To exit the communications
mode follow screen prompt or reset the VIAFLO pipette
). Serial communication further allows (in addition to
(13
the functions of Bluetooth):
• Firmware updates
• Bi-directional communication from PC to pipette.
On/Off
communication has an open field connectivity of about
100 meters. It can be used for:
• Create and manage custom pipetting programs
• Image downloads
• Service history tracking
Description Range
-
information for your VIAFLO Electronic Pipettes.
•Use the Touch wheel to highlight a character and
press OK. You can press to Delete the last character entered. After entering the desired text, press to
Save.
18 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Installation
3.3.5 Help Language
Help
Language
Help
Language
3.3.6 Write protect
Select this option to protect programs and menu options from inadvertent modification.
The pipetting programs can still be used.
Write
protect
Keep the password in a safe place.
Description Range
You can choose the language in which all help screens
are displayed (English, French, German, Spanish,
simplified Chinese, Japanese). Scroll to the desired
language and press to Save.
Description Range
Select an option and press OK to switch protection On or
Off:
• Standard programs
• Custom programs
• Calibration
• Too lbo x
• VOYAGER
• Password protect: Protect the access to the write pro-
tect menu by selecting “On”.
• Edit password, if password protect is “On”. To enter a
password use the Touch wheel to highlight a charac-
ter and press OK. Press to save the password. The
password must be entered before you can access the
write protect menu.
-
On/Off
3.3.7 Password key
This menu displays the encrypted password, if set. Should you lose your password,
contact INTEGRA Biosciences to retrieve your password using these numbers.
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VIAFLO Pipettes – Operating instructions V05 Operation
4 Operation
4.1 Turn on/off the device
Turn on:
Press and release the Run key (7
AUTION
C
Remove hands from the Tri-lobe tip interface (12) at switch on and during
homing. Also do not touch the touch wheel, because it is calibrated during
the start up process.
The pipette flashes the startup screen and performs a full motor homing routine, ensuring
the motor is in the run position. “Home” is the base point for the pipette. Homing is the
process whereby the pipette motor moves the piston(s) to a sensor position. This position
ensures that no liquid remains in the tips. For the VOYAGER, homing also includes the tip
spacing motor. After homing, the tips move to the last position they occupied.
After homing the menu last used is displayed. Press the Back button (2
as necessary to return to the Main menu.
Turn off:
To turn off the pipette, press and hold the Back button (2
OTE
N
The VIAFLO Electronic Pipettes will dim and tu rn off automatically after a
preset duration of inactivity. This duration is 5 minutes, by default, and
configurable with the Toolbox (see “3.3.1 Preferences” on page 16).
) to turn on the pipette.
) as many times
) for 3 seconds.
4.2 Attaching and removing GripTips
CAUTION
To ensure optimal performance of your VIAFLO Electronic Pipettes always
use GripTips which have been designated for the use with the VIAFLO
Electronic Pipettes, see “8.2
The unique tri-lobe design of the VIAFLO Electronic Pipettes reduces attachment and
ejection forces, ensures a perfect fitting to prevent the tips from falling off and to provide
a perfect sealing. On a multichannel pipette all tips sit on the same height.
Tri-lobes minimize contact surface and temporarily deforms the tip
shaft
Shoulder provides a positive stop to prevent over tightening and all
tips seal at the same height
O-ring provides forgiving and robust seal surface for the pipet tip
20 INTEGRA Biosciences AG
Consumables” on page 64.
VIAFLO Pipettes – Operating instructions V05 Operation
Attach the tips:
When loading tip(s), press the pipette into the appropriate GripTip(s) until you hear and
“feel” a click indicating that a seal has been achieved. Once you feel the click, stop
applying pressure.
Discard your used tips:
If liquid is in the tips, empty them by pressing the Purge button (6
removed by pressing the Tip ejector (8
).
). Tips are easily
4.3 Start pipetting
4.3.1 Pipetting
Use the Touch wheel (3
Selected parameters for the action you are about to perform will be displayed on the Run
screen.
) to scroll to your desired pipetting mode and press OK (4).
Insert the tips into the liquid to be transferred. Press
and release the Run key (7
selected in the first step of your protocol (shown on
the Run screen).
To execute subsequent steps, press the Run key.
) to aspirate the volume
For a detailed description see “5.2 Detailed description of pipetting modes” on page 32
You can change the parameters of your pipetting mode at any time, see the following
sections.
4.3.2 Blowout modes
During the last dispense of a program, a blowout is performed automatically. Liquid may
be aspirated back into the tips when the pistons move back to the home position, a
process called blowin. There are two ways to perform the blowin:
• Automatic blowout: Pressing (and releasing) the Run key starts the dispense with
blowout and automatic blowin. You can choose a timed delay between the blowout and
the blowin, see “Pipetting - Delayed blowin” under “3.3.1
• Two-step blowout: Perform a two-step blowout to manually delay the blowin:
- Press and hold
- Remove the tips from the target vessel.
- Release the Run key to start blowin.
www.integra-biosciences.com 21
the Run key to start dispense with blowout.
Preferences” on page 16.
.
VIAFLO Pipettes – Operating instructions V05 Operation
4.3.3 Recommendations for pipetting
INTEGRA Biosciences recommends the following techniques for enhancing pipetting
results. These techniques are consistent with ISO standard 8655-2.
• It is best to immerse the GripTips just enough in liquid (2-3 mm) to allow the desired
volume to be aspirated.
• Always prewet GripTips. After loading tips onto your pipette, aspirate and dispense the
full volume 2-3 times to coat the inside of pipet tips. Pre-wetting ensures that the liquid
and air inside the tips are at equal temperature and the dead air space is humidified.
• VIAFLO Electronic Pipettes is an air displacement pipette. To properly dispense liquids,
ensure that the pipette tip is at a 0-30° angle against the wall of the container or well.
After dispense you must touch GripTips against wall or dip them into the liquid after a
dispense. This process is referred to as “touching off” or “tip touch” and prevents liquid
from clinging to the pipette tips.
• In programs such as Repeat Dispense, a first and last dispense can be programmed.
These two dispenses are not used and are dispensed into the waste as they contain the
accumulated pipetting errors. Using a first and last dispense is recommended if
accuracy and precision are of high importance.
• Viscous samples should be aspirated and dispensed at the slowest speeds to ensure
accurate pipetting. In addition, the pipetting mode “Reverse pipet” can be used to
optimize pipetting results with viscous samples.
• For pipetting liquids with high vapor pressures (such as methanol or ethanol), use
relatively fast pipetting speeds and avoid prolonged pauses after aspiration.
• Calibrate based on fluid type. VIAFLO Electronic Pipettes is tested and calibrated at the
factory for use with distilled water at room temperature. It may be necessary to recalibrate your VIAFLO Electronic Pipettes if the liquid to be used has different physical
properties (specific gravity and vapor pressure) than water. Calibration mode can be
accessed in the Toolbox menu.
ARNING
W
Avoid pipetting for extended periods. To minimize the risk of repetitive strain
injury, include pauses of several minutes.
22 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Operation
4.4 Pipetting options and settings
4.4.1 Edit option
The Edit option is available for each mode. It enables you to access the variables that you
can adjust for a pipetting mode. These variables include Speed, Volume, Pace, Count,
Mix Cycles, Rows and Direction. Additional steps include First dispense, Last dispense,
Air gap, Aspirate speed, Dispense speed, etc.
Select a pipetting mode. Then, select Edit on the list
of options and press OK. A list of associated steps is
displayed. For example, if selecting Edit on the
Repeat Dispense screen, the modifiable steps
associated with Repeat Dispense are displayed.
4.4.2 Volume selection
To change a volume select the Edit option and press OK. The adjustable volumes are
displayed.
Use the Tou c h w heel to highlight the volume you
want to change (Aspirate, Dispense, Mix, or Air Gap).
Press OK and a Volume setting “dial” is displayed.
Use the Touch wheel to change the volume. Press
OK to confirm your volume selection and to save.
NOTE
Use the arrow buttons to change the volume in coarse or fine incre ments.
Select COARSE (with ) to change the volume in larger increments. Select
FINE (with ) to change the volume in smaller increments. The increment
sizes vary based on the pipette volume range, as shown under “7.4
Specifications” on page 60.
Define and select favorite volumes
You can define, save, and select up to ten favorite volumes for quick access. These
volumes can only be within the pipette volume range.
There are two ways to access and customize the list of favorite volumes:
• When in Pipet mode, use the Touch wheel to highlight Favorites and press OK.
• When in other modes, select the Edit option and press OK. The steps with volumes to
be adjusted are displayed. Use the Touch wheel to highlight the desired volume and
press Favorites to display the list of favorite volumes.
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Pipette
VIAFLO Pipettes – Operating instructions V05 Operation
Use the Touch wheel to highlight the desired volume
and press Select. Alternatively, modify a volume
by pressing Edit.
Save your setting .
4.4.3 Speed selection
The speed option controls the speed at which liquid is aspirated, dispensed, or mixed in
each mode. Speed can be set as a value from 1 (slowest) to 10 (fastest), see also “7.3
Pipetting speed” on page 59.
When in in any pipetting mode, use the Touch wheel
to highlight the Speed option and press OK. Select
the speed and press OK to save your setting.
Speed may be changed in most
Edit menus. Scroll to the Speed and
press OK. Choose the speed,
press OK, and press to save
your selection.
The speeds selected in each mode (i.e., Pipet, Repeat Dispense, etc.) are stored for that
mode only.
Speeds can be set independently for each operation (Aspirate, Dispense, Mix).
OTE
N
Viscous samples should be aspirated and dispensed at the slowest speeds
to ensure accurate pipetting.
To dispense liquids with low viscosity and high vapor pressure, such as
alcohol, use relative fast pipetting speeds and avoid prolonged pauses for
aspiration.
24 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Operation
4.4.4 Pace
The Pace option sets the time gap between dispenses in repeat pipetting. Pace is used in
the Repeat Dispense and Variable Dispense modes. While you press and hold the Run
key, the pipettor will dispense multiple programmed volumes with the selected pace.
Release the Run key to stop the paced dispense. Press Run to continue dispensing.
Use the Touch wheel to select the desired Pace
option and press OK.
Select the pace, from None, 1 (slowest) to 9 (fastest).
Press OK to save your setting.
4.4.5 Count, Mix Cycle and Rows
The Count, Mix Cycle, and Rows steps are used in various modes, see “5.2 Detailed
description of pipetting modes” on page 32. Each is accessed with the Edit option. Use
the Touch wheel to highlight the step and press OK.
Count sets the number of dispensing steps. Mix Cycle sets the number of mixes. In serial
dilution mode, Columns sets the number of columns. A column indicator will notify the
number of dilutions performed. Columns (first number) and Mix Cycles (second number)
are tracked on the display. Mix Cycles are shown in red when mixing. A green dot on the
column number indicates the active program step.
Select a desired value. Press OK and then press to save your setting(s).
4.4.6 Help
The Help information available for each mode describes the mode operation. You can
select Help in two ways:
While in the Main menu, highlight a pipet mode, then
press to select the Help option.
While in a pipet mode, find the Help option from the
list and press OK.
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VIAFLO Pipettes – Operating instructions V05 Operation
4.5 VOYAGER tip spacing
The VOYAGER multichannel pipettes provides you with the ability to vary tip spacing. The
tip spacing is expressed in millimeters and represents the distance between adjacent tips.
This spacing can be adjusted in either 0.1 mm or 0.25 mm increments depending on the
instrument selected.
OTE
N
Tip spacing is only displayed if “Tip Spacing” is switched on under Toolbo x Preferences - Main Menu.
4.5.1 Set the number of tip positions and the tip spacing
It is possible to set two or three tip spacing values based on labware types to be used.
Use the touch wheel to highlight Tip Spacing on the
Main Menu. Press OK. The tip spacing menu
displays:
• The number of positions the VOYAGER will move
between (2 or 3).
• The First, Middle, and Last positions.
• The current spacing that the tips are in.
To change the number of positions to move between, use the touch wheel to scroll to
Positions and highlight either 2 or 3.
Press OK to toggle between 2 or 3 positions. Press to Save this selection. If 2 positions
are chosen, the Middle position is grayed out.
To change the tip spacing use the touch wheel to
scroll to the position to be changed. Press OK and
the Tip Spacing menu appears.
Press (Close) to reduce or (Open) to increase
the tip spacing incrementally. The tips will physically
move allowing you to visually match the tip spacing
to the target vessels. When the desired tip spacing is
achieved, press OK.
Make the desired changes to all positions. Upon completion, press to Save all the
selections.
26 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Operation
4.5.2 Tip spacing operation
Select the pipetting function of interest (Pipet, Repeat Dispense, Sample Dilute, etc.).
Then, the bottom of the screen displays the Tip Spacing that the instrument is set at.
The First, Middle, and Last positions are displayed from left to right.
In the adjacent figure, 9.0 mm is the First position, 11.5 mm is the
Middle position, and 14.0 mm is the Last position. The current position
is highlighted in yellow.
Press or to move the tip position to the next value. Pressing or
moves the tip spacing selection in a circular loop.
This process can be executed at any step in the program.
4.5.3 Homing the tips
Homing the tips may be necessary if the tip spacing motor was restricted from achieving
its proper position. Every time the pipette is turned on, the tip spacing motor homes and
returns to the position that it was last in.
You also may home the tip spacing motor as needed.
To do so, press on the Tip Spacing menu to select the Home Tips
option
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VIAFLO Pipettes – Operating instructions V05 Operation
4.6 Troubleshooting/FAQ
4.6.1 General
Problem Probable cause Remedy
Pipette leaks or
does not fill with
liquid after
reassembly.
Leakage. • Tip incorrectly attached.
Dispense results
are inaccurate.
No dispensing/
aspirating.
Droplets on the
tips.
• O-ring was not properly
installed.
• During reassembly, the O-ring
was damaged.
• Foreign particles between tip
and tip cone.
• Foreign particles between the
piston, the O-ring, and the
cylinder.
• Damaged red O-ring.
• Unsuitable calibration.
• Incorrect aspirate and dispense speed.
• Piston stuck or not connected.
• Motor not running.
• O-ring not installed.
• Temperature of liquid differs
from that of air inside the tips.
• Liquid of low viscosity and
high vapor pressure.
• Replace the tip fitting O-ring,
see 6.3.2.
• Attach a new tip.
• Clean tip cones. Attach new
tips.
• Change the red O-ring, see
.
6.3.2
• If leak persists, contact service.
• Recalibrate with the liquids in
question.
• Adjust aspiration and dispense
speed depending on liquid:
- High viscosity liquids may
require calibration.
- High vapor pressure liquids
may require pre-wetting.
• Contact service.
• Pre-wet tips up to 3 times.
• Increase dispensing speed.
28 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Operation
4.6.2 Electronic
Problem Probable cause Remedy
When pressing Run,
a “Battery Low”
message appears
on the Run screen.
Display turns off
completely.
Spinner response is
erratic and uncontrollable.
Displayed characters are
scrambled.
Battery charging
indicator is not pulsing while on the
stand. Unit does not
turn on when placed
on the charging
stand.
Error message is
displayed: “Home
Found” or “Home
Not Found.”
• Low battery. • Re-charge the battery in order to
resume pipetting operation.
• Plug the power cord into the
pipette. Note: It takes 15-30 seconds to store enough voltage to
operate the pipette.
• Dead battery. • Charge the battery with a power
cord or charge stand.
• Sometimes a dead battery can
be saved by unplugging the battery for 5 seconds. Then plug in
battery and try to turn on pipette.
If it powers up, immediately connect to power supply. If pipette
does not turn on after this procedure, replace with new battery.
• A finger was on the spinner
when the pipette was turned
on.
• Sensitivity setting is not set
correctly.
• Unknown. • Reset the pipette.
• Charge stand pins are out of
place.
• Indicates too much friction
was encountered during
operation.
• Indicates possible motor failure.
• Reset the pipette without touching the spinner during reset.
• Adjust the spinner sensitivity with
Toolbox, Preferences, Spinner,
see 3.3.1
• Check that both charge stand
conductor pins are at the same
height.
• Make sure the charger is
plugged in.
• Reset the pipette.
• If problem persists, please contact service for technical assistance.
.
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VIAFLO Pipettes – Operating instructions V05 Operation
Problem Probable cause Remedy
Motor sounds rough
and aspiration is
very slow.
• Pipette has been set to the
slowest speed.
• Verify the speed setting. The
slowest speed setting normally
causes the motor to run slowly
and louder than normal.
30 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5 Pipetting modes
This chapter describes how to program the VIAFLO Electronic Pipettes in two ways:
• Function-based pipetting modes: You can select from ten predefined pipetting
modes that you can quickly and easily edit and execute. They are described in the following sections.
• Custom step-based programming mode: You can create and store up to twenty
multi-stepped pipetting protocols on the pipettor using the five basic functions of “Aspirate, Mix, Dispense, Purge and Prompt” presented in “5.2
pipetting modes” on page 32. The custom programming mode is described in “5.3
Custom step-based programming mode” on page 41
5.1 Overview pipetting modes
The table below provides an overview of the selectable pipetting modes. All modes are
accessed from the Main Menu. Use the Touch wheel to scroll to your desired pipetting
mode.
Pipetting mode Description
Pipet Allows liquid transfers when aspirate and dispense volumes are
equal.
Repeat Dispense Allows dispensing multiple aliquots of the same volume without
refilling the tips after each dispense for fast microplate filling and
processing.
Sample Dilute Allows aspirating of sample and diluent divided by a defined air
gap into one tip, followed by a complete dispense.
Pipet/Mix Allows multiple mixing by aspiration and dispensing of defined
volume for automatic re-suspension of pellets.
Manual Pipet Allows the operator to control the aspiration and dispensing up to
the set volume.
Reverse Pipet Allows liquid transfers of viscous or high vapor pressure liquids
by preventing introduction of any air into the sample. The
aspiration volume is higher than the volume to be dispensed.
Variable Dispense Allows dispensing multiple aliquots of different volumes.
Variable Aspirate Allows aspirating multiple aliquots of different volumes.
Sample Dilute/Mix Allows aspirating two liquids separated by an air gap followed by
a complete dispense and Mix step.
Serial Dilution Allows aspirating a transfer volume followed by a mix. Columns
and Mix Cycles are tracked on the display.
Custom Allows to create and store of up to 20 multi-stepped pipetting
protocols.
Press the OK to access the pipetting mode and to start defining parameters.
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Detailed description of
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2 Detailed description of pipetting modes
The VIAFLO Electronic Pipettes offers ten predefined pipetting modes. Most liquid
handling protocols can be easily accommodated using one or more of these modes. The
options and steps of the different pipetting modes are described in the following
subsections.
5.2.1 Pipet mode
Application: Use this mode for quick transfer of liquid to or from microplates.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is equal to the dispense
volume.
Speed Sets speed for the current pipetting step (1 = low, 10 = fast).
Favorites Defines up to 10 favorite volumes
Help Help information is available for each mode.
Operation:
• With the tip(s) in liquid, press and release the Run key to aspirate.
• With the tip(s) in the destination plate, press and hold the Run key to execute the dispense and perform a two-step blowout, see “4.3.2
• When the tips are removed from the target plate, release the Run key.
Blowout modes” on page 21.
32 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.2 Repeat dispense mode
Application: This mode can be used for fast reagent addition to microplates from one
source container. You can dispense a large aspirated volume of liquid in multiple aliquots
to multiple targets.
Options Steps Description
Edit Dispense Sets the volume for repetitive dispensing. The aspirated
volume is calculated automatically.
First
Dispense
Last Dispense A last-dispense volume can be selected independently to
Count The maximum number of dispenses possible (count) is
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = low, 10 = fast).
Speed Sets speed of the current pipetting step.
Pace Sets the time duration between dispenses, if keeping Run
Help Help information is available for each mode.
Operation:
• With the tip(s) in liquid, press and release the Run key to initiate the aspirate step.
• Press and release the Run key for every dispense. Alternatively, press and hold Run
to execute paced dispenses. The dispense number is shown on the display.
• The pipettor will stop paced dispenses when it reaches the Last Dispense. This aliquot
contains the accumulated error from all prior dispenses. You can choose to use this
Last Dispense or discard it. During the Last dispense, press and hold the Run key to
perform a two-step blowout.
A pre-dispense volume can be selected independently to
improve accuracy and precision. The dispense is discarded.
improve accuracy and precision. The dispense is discarded.
calculated automatically. This count may be reduced to the
desired number.
key pressed.
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VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.3 Sample dilute mode
Application: Accomplish accurate sample dilutions by using diluent to “chase” small
sample volumes from the pipet tips. An air gap keeps liquid separated in the tips and helps
to minimize carryover of diluent when aspirating the sample.
Options Steps Description
Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip.
Air Gap Sets the volume of the air gap to keep both liquids separated.
Aspirate 2 Sets the volume of the sample in the tip.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = low, 10 = fast).
Speed Sets speed of the current pipetting step.
Help Help information is available for each mode.
Operation:
• Press and release the Run key to initiate each aspiration (remove tips from liquid for
air-gap aspiration).
• Press and hold the Run key to perform a two-step blowout. The entire tip contents will
be dispensed together.
5.2.4 Pipet/mix mode
Application: Use this mode when mixing is required immediately after transfer of liquid.
This mode saves a programming step by incorporating the mix option after dispensing.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is equal to the dispense
volume.
Mix Sets the mixing volume after dispensing.
Mix Cycles Sets the number of mix cycles.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Mix Speed Sets speed uniquely for mixing (1 = low, 10 = fast).
Speed Sets speed of the current pipetting step.
Help Help information is available for each mode.
Operation:
• Press and release the Run key to initiate the aspiration.
• Press and release the Run key to dispense. Mixing occurs automatically after the dispense step.
• Upon completing the desired number of mixes, a blowout is initiated automatically
prompting you to remove the tip(s) from the liquid and press Run to complete the
blowout.
34 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.5 Manual pipet mode
Application: This mode can be used when the aspiration volume is not defined or
unknown. You have control over the aspiration and dispense steps and can view the
display to confirm how much liquid has been aspirated or dispensed. Manual control over
the dispense steps is perfect for performing Titrations or for controlling the loading of
samples in gel lanes.
Options Steps Description
Edit Aspirate Sets the aspiration or dispensing volume. Toggle between
Aspirate and Dispense using the Direction menu option.
Speed Sets speed of the current pipetting step (1 = low, 10 = fast).
Direction Changes the direction of pipetting between aspiration and
dispensing.
Favorites Defines up to 10 favorite volumes
Help Help information is available for each mode.
Operation:
• When aspirating, the motor will stop when you release the Run key or when the programmed aspirate volume is reached.
• You can change pipetting direction at any time even if aspiration volume is not
reached. Change the direction of pipetting by pressing OK on the Direction option.
The notation on the display changes between (Aspirate) and (Dispense).
• Titrations can be performed by dispensing in this mode. The volume remaining in the
tip(s) is always actively displayed.
OTE
N
Use slower pipetting speeds (1-5) for better control and resolution.
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VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.6 Reverse pipet mode
Application: Use this mode when performing reagent addition where a blowout is not
desired when dispensing, e.g. for solutions with high viscosity or tendency to foam. The
dispense method used in this mode, prevents introduction of any air into the sample.
Options Steps Description
Edit Aspirate Sets the aspiration and dispense volume.
Last Dispense Sets the volume to leave in the tip until final blowout.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = low, 10 = fast).
Speed Sets speed of the current pipetting step.
Help Help information is available for each mode.
Operation:
• Press and release the Run key to initiate the aspiration. The total volume aspirated is
the sum of desired dispense volume and last dispense volume.
• Depending on whether you press and release or hold the Run key at the start of a dispense step, you can repeat the aspirate and dispense process:
- Press and hold the Run key: Dispense the programmed volume without a blowout,
keeping the Last Dispense volume in the tip. Upon release of the Run key, you can
aspirate the programmed volume again and repeat the dispense process as many
times as needed.
- Press and release the Run key: Finish the aspirate and dispense process. The
programmed volume will be dispensed. Last Dispense is the next active step.
• To purge the Last Dispense volume with a two-step blowout, press and hold the Run
key.
36 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.7 Variable dispense mode
Application: Use this mode when differing dispense volumes are required. This mode
could be used to quickly set up a dilution series in plates or for feeding similar samples to
different assay plates where different sample volumes are needed.
Options Steps Description
Edit Count Sets the total number of dispensing steps.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = low, 10 = fast).
Dispense
1...5
Speed Sets speed of the current pipetting step.
Pace Sets the time interval between dispenses in repeat pipetting
Help Help information is available for each mode.
Operation:
• Press and release the Run key to initiate the aspiration of total volume.
• Press and release the Run key to initiate each subsequent dispense. The pipettor
stops and beeps when ready for the Last Dispense step, i.e. to purge the calculated
waste volume amount.
• Alternatively, press and hold the Run key to execute paced dispenses. The pipettor
stops paced dispensing when it reaches the Last Dispense. This aliquot contains the
accumulated error from all prior dispenses. You can choose to use this Last Dispense
or discard it.
• During the Last dispense, press and hold the Run key to perform a two-step blowout.
Sets up to 5 different volumes for repeated dispensing. The
total volume is automatically calculated.
(1 = long, 9 = short).
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VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.8 Variable aspirate mode
Application: This mode can be used for a variety of collection applications where the
aspiration volume is well known. This mode is also suited for supernatant collection in
microplates.
Options Steps Description
Edit Count Sets the total number of aspirating steps.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = low, 10 = fast).
Aspirate 1...5 Sets up to 5 different volumes used for sequentially aspirating
(in the same tip) followed by a single dispense.
Speed Sets speed of the current pipetting step.
Help Help information is available for each mode.
Operation:
• With the tip(s) in liquid, press and release the Run key to initiate the first aspiration
volume. Again in liquid, press and release the Run key to initiate the second aspiration volume, etc.
• Press and hold the Run key to start Dispense and perform a two-step blowout.
38 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.9 Sample dilute/mix mode
Application: Use this mode to perform sample dilutions where mixing of sample and
diluent is required. This mode could also be used to introduce and mix diluent and sample
to the first column of a serial dilution plate.
Options Steps Description
Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip.
Air Gap Sets the volume of the air gap to keep both liquids separated.
Aspirate 2 Sets the volume of the sample in the tip.
Mix Sets the mixing volume after dispensing.
Mix Cycles Sets the number of mix cycles.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Mix Speed Sets speed uniquely for mixing (1 = low, 10 = fast).
Speed Sets speed of the current pipetting step.
Help Help information is available for each mode.
Operation:
• With the tip(s) in liquid, press and release the Run key to initiate aspiration 1. With the
tip(s) out of the liquid, press and release the Run key for the Air Gap. Again in liquid,
press and release the Run key to initiate aspiration 2.
• Press and release the Run key to dispense the entire tip contents and begin the mixing routine. Upon completing the desired number of mixes, a blowout occurs automatically. Remove tips from liquid and press and release the Run key to complete the
blowout.
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VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.2.10 Serial dilution mode
Application: Use this mode to perform serial dilutions. The Serial Dilution mode enables
aspiration of a specific volume followed by a mix sequence and ending with the original
aspiration volume in the tips.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is identical to the dispense
volume.
Mix Sets the mixing volume after dispensing.
Mix Cycles Sets the number of mix cycles.
Columns Sets the number of columns. A column indicator will notify the
number of dilutions performed.
Asp. Speed Sets speed uniquely for aspirating (1 = low, 10 = fast).
Mix Speed Sets speed uniquely for mixing (1 = low, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = low, 10 = fast).
Speed Sets speed of the current pipetting step.
Help Help information is available for each mode.
Operation:
• With the tip(s) in liquid, press and release the Run key to initiate the aspiration of the
reagent.
• Submerge the GripTips in the liquid located in the first row of the plate. Press and
release the Run key to start the dispense and mix sequence. Proceed with the rest of
the rows.
• Rows (first number) and Mix Cycles (second number) are tracked on the display. Mix
Cycles are shown in red when mixing. A green dot on the row number indicates the
active program step.
40 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.3 Custom step-based programming mode
Application: Use the Custom program mode to create personalized pipetting tasks. Up
to twenty programs can be stored.
5.3.1 Create a custom program
From the Main Menu select “Custom” to create a personalized protocol. Programs can
contain up to 98 individual steps based upon the following basic operations: Aspirate,
Dispense, Mix, Purge, Prompt and Tip spacing.
OTE
N
We recommend creating custom programs on a PC with the VIALINK
software, see also “3.3.3 Communications” on page 18.
Press to create a new program. The program is
assigned a name automatically (e. g. Custom 1). The
custom programs can later be renamed with
alphanumeric characters.
A Custom program must begin with an Aspirate, Mix, Prompt or Tip spacing. Use the
Touch wheel to select the first step, e.g. Aspirate, and press OK.
Set the volume value and press OK.
Set the speed for that step and press OK.
Next, you have the options of adding a new step, editing a step, or deleting a step.
Select “New step” and press OK.
Use the Touch wheel to select the position where the new step
should be inserted, press OK and select an operation.
When starting with an “Aspirate” step followed by a “Mix” step, the tips contain the aspirate
volume after completing the last mix cycle. When starting with a “Mix” step, the tips are
emptied upon completion of the last mixing cycle.
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VIAFLO Pipettes – Operating instructions V05 Pipetting modes
Define step by step a new custom program.
Step Description
Mix Sets the mixing volume after dispensing.
Purge Purges all remaining liquid currently in the GripTips. A “Purge” step
is automatically integrated at the end of a program if the last
programming step leaves liquid in the tips.
Prompt A prompt is any message that is displayed during the program. Use
the Touch wheel to select one of 3 lines and press OK. Highlight a
character that you want to use and press OK.
After you enter the desired text, press to Save.
To save and store a Custom program, press .
5.3.2 Program tip adjustments in a custom program
Select “New step” and select the position where the new step should be inserted. Press
OK.
Scroll down to highlight Tip Spacing and press OK.
Either select one of the three favorite Tip Spacings by
using the touch wheel or press and to set the
desired Tip spacing.
5.3.3 Modify existing programs
At the Custom program display, use the Touch wheel to highlight an
existing program. Press the OK button to select it. Select an option
(View/Edit, Delete, Copy, Name) to modify the program.
Press Back to return to the list of Custom programs. To run the program, press .
42 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Pipetting modes
5.3.4 Example of custom mode
Application: The task is to combine 2 different liquids in a 96 well plate for a kinetic assay
and then mixing it to achieve a homogenous solution. The custom program would be set
up as followed:
Program step Action
1. Aspirate liquid 1: 160 µl (e.g. diluent) With tips in liquid 1 press Run key.
2. Aspirate air: 20 µl Move tips out of liquid and press Run key.
3. Aspirate liquid 2: 50 µl (e.g. reagent) With the tips in liquid 2 press Run key.
4. Dispense: 230 µl Press and hold Run key until liquid is
dispensed and tips are removed from the
liquid (two-step blowout).
5. Mix 3x: 200 µl Press Run key.
Purge does not need to be programmed. The residual liquid is dispensed into the waste
container. Press and hold Run key until liquid is purged and tips are removed from the
liquid (two-step blowout). For a detailed description see “4.3.2
page 21.
Blowout modes” on
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VIAFLO Pipettes – Operating instructions V05 Maintenance
a
b
6 Maintenance
WARNING
Always turn off power and disconnect the VIAFLO Electronic Pipettes from
the mains when carrying out maintenance work.
6.1 Cleaning
The materials used on the exterior of the VIAFLO Electronic Pipettes support regular
cleaning intervals. Clean the external components with a lint-free cloth lightly soaked with
mild soap solution in distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never
use acetone or other solvents.
ARNING
W
Do not immerse the entire pipette into a cleaning so lution or spray cleani ng
solution directly onto the exterior body of the pipette as this can potentially
damage internal electronics.
If liquid ever enters the internals of VIAFLO Electronic Pipettes, please
contact your service technician.
Follow the instructions below for disassembly necessary for trouble shooting and
autoclaving.
6.1.1 Assembly of single channel pipettes
Disassembly
Disassemble the lower portion of the single channel pipette as follows:
1) Ensure the pipette is in the “home” or down
position (at the end of a pipetting cycle).
2) Turn off power and unplug the power supply.
3) Unscrew clockwise to remove the Cylinder
Assembly (a) from the pipette. Slide the Cylinder
Assembly down from the pipette body. This will
expose the Piston Assembly (b)
44 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Maintenance
b
c
d
e
a
f
g
h
4) Slide the following components from the Piston:
• O-Ring (black) and Seal (white) Assembly (e)
• Seal Retainer Spring (d)
• Seal Retainer (c)
Set these components aside or place them in an
autoclave pouch.
5) Separate the Piston Assembly (b) from the upper
portion of the pipette. The Piston is held in place
by a small magnet.
6) With the Cylinder Assembly (a) in hand, turn
counterclockwise to remove the black Sealing
Ring (f) that holds the Ejector Sleeve (h) in place.
7) Remove the Ejector Sleeve (h) from the Cylinder
Assembly.
8) Remove the Ejector Sleeve Spring (inside, g).
9) Set all components aside or place them in an
autoclave pouch.
Reassembly
Before reassembling the pipette, check each component for lint or dust particles. It is
recommended to replace the O-Ring (black) and Seal (white) Assembly (e) before
reassembly. The O-Ring and Seal slide on/off the piston and can be replaced easily.
1) Lightly lubricate the piston and seals (see “6.3.3
Piston (b) at the small magnet to connect the Piston to the upper portion of the pipette.
2) Slide the Seal Retainer (c) onto the Piston and up into the pipette.The end of the Seal
Retainer that has an extended edge (lip) should be closest to the upper portion of the
pipette (over the magnet).
3) Slide the Seal Retainer Spring (d) onto the Piston. It will rest against the Seal Retainer.
4) Slide the lightly lubricated Seal (white) and O-Ring (black) Assembly (c) onto the
Piston. The white Seal will be closest to the Seal Retainer Spring. Be sure the black
O-Ring is securely in place on the white Seal.
5) With the Ejector Sleeve (h) in hand, slide the Ejector Sleeve Spring (g) onto the top of
the Sleeve.
6) Slide the Ejector Sleeve into the Cylinder Assembly (a).
7) Position the black Sealing Ring (f) over the Ejector Sleeve (h) at the top of the Cylinder
Assembly. Turn clockwise until the Sealing Ring snaps into place and secures the
Ejector Sleeve in the Cylinder Assembly.
8) Slide the Cylinder Assembly over the Piston.
Lubrication” on page 50). Position the
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VIAFLO Pipettes – Operating instructions V05 Maintenance
9) Screw counterclockwise to attach the Cylinder Assembly to the body of the pipette.
Check the pipette performance after reassembly.
6.1.2 Assembly of multichannel fixed spacing pipettes
Before reassembling the pipette, check each component for lint or dust particles. It is
recommended to replace the O-Rings, see “6.3.2
page 49.
Step 1
Disassembly:
Rotate counter-clockwise to remove the lower housing.
Reassembly:
Rotate clockwise until a stop is felt; then back off to align.
Step 2
Disassembly:
Gently pull to separate the upper and lower housing to expose the
ball and socket.
Reassembly:
Gently push together the upper and lower assembly.
Step 3
Disassembly:
Disengage the ball and socket to separate the parts.
Reassembly:
Reengage the ball and socket to reconnect.
Changing O-rings of tip fittings” on
Step 4
Disassembly:
Remove the aluminum ring from the upper assembly.
Reassembly:
Slide the aluminum ring back onto the upper assembly. (The
flanged surface goes into the upper assembly.)
46 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Maintenance
a
b
Step 5
Disassembly:
Remove the chrome ring.
Reassembly:
Slide the chrome ring back into place (with the stepped surface up).
Step 6
Disassembly:
Carefully remove the volume label to expose the screw. (Save the
label for reassembly.)
Reassembly:
Reattach the volume label where previously removed.
Step 7
Disassembly:
Remove the two small screws.
Reassembly:
Replace the two small screws on the front housing where the
volume label had been removed (if not completed in the previous
reassembly step).
Step 8
Disassembly:
Remove the two screws from the rear housing.
Reassembly:
Replace the small screws from the rear housing.
Step 9
Disassembly:
Separate the housing from the cylinder/piston assembly.
Reassembly:
Place the rear housing (b) back onto the front housing/piston
assembly (a).
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VIAFLO Pipettes – Operating instructions V05 Maintenance
c
d
Step 10
Disassembly:
Remove the foam tape from the front and rear (carefully note the
position of the tape and save for reassembly in exactly the same
position, c).
Reassembly:
Reattach the foam tape to the front and rear housing where it was
removed previously. Place the piston assembly in the front housing,
registering on the two bosses. Turn over and replace the two small
screws (do not over tighten).
Step 11
Disassembly:
Unscrew the manifold screws one full turn (2 to 6 screws depending
on volume size) and pull the piston plate (d) to the up position.
Reassembly:
Be sure the piston plate is in the up position and tighten the manifold
screws exactly one full turn (you should feel light pressure on the
screws).
6.2 Sterilization
If the surface of the VIAFLO Electronic Pipettes have been in contact with biohazardous
material, they must be decontaminated in accordance to good laboratory practice. Whisk
and wipe the surface with a lint-free cloth, lightly soaked e. g. with the following
disinfectants:
• Ethanol 70 %
• Microcide SQ 1:64
• Glutaraldehyde solution 4%
• Virkon solution 1-3%
Follow the instructions provided with the reagents.
It is not recommended to autoclave the VIAFLO pipettes except for decontamination
before sending them for service and repair. Only the lower assembly of the VIAFLO single
and multi channel pipettes can be autoclaved.
ARNING
W
A service is required after autoclaving the VIAFLO pipettes!
Do not autoclave the entire unit. The extreme heat can damage the di splay
and other electrical components. VOYAGER pipettes can NOT be
autoclaved, they might be damaged!
As-found calibration (measurement report, indicating “before” data) is not
possible after autoclaving!
48 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Maintenance
6.2.1 Autoclaving the disassembled components
Place the disassembled components (see 6.1
) to steam autoclave in an autoclave pouch:
Single channel Multichannel fixed spacing
You may autoclave the components at 121°C, 1 bar overpressure for 20 minutes.
After autoclaving send the pipette for service.
6.3 Servicing
6.3.1 Shipping to INTEGRA Biosciences
For any service or repairs, please contact your local service technician.
ARNING
W
If working with infectious materials, e. g. human pathogens, VIAFLO pipettes
need to be decontaminated before sending them to service and the
declaration on the absence of health hazards must be signed. This is
necessary to protect service personnel.
6.3.2 Changing O-rings of tip fittings
300 µl, 1250 µl and 5000 µl VIAFLO pipettes feature tip fittings with red O-rings. This Oring is used to seal against the inside wall of GripTips and serve to reduce the required tip
loading and ejecting forces.
O-rings are made of durable silicone. If necessary, e.g. in case of a leakage due to
damaged O-ring, you can replace these O-rings. A set of spare O-rings and an O-ring
removal tool are included with the large volume multi-channel pipettes and can be ordered
separately, see “8
Accessories” on page 64.
ARNING
W
Avoid mechanical damage of the tip fittings.
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VIAFLO Pipettes – Operating instructions V05 Maintenance
a
b
b
c
a
d
e
For 300 µl or 1250 µl pipettes choose the side of the O-ring removal
tool corresponding to the size of the pipette cylinder (300 µl or
1250 µl). Slide the O-Ring removal tool sidewards onto the tip fitting
until the O-ring (a) builds a loop. Cut the O-ring with a fine scissor
and remove it.
For 5000 µl pipettes use forceps to remove the O-ring.
Slide a new O-ring over the tip fitting (b).
6.3.3 Lubrication
The internal seals and O-rings are subject of very slow wear out. An undamaged thin
lubricant film is important to keep the seals tight. The lubricant recommended depends on
the VIAFLO pipette size, see “8.2 Consumables” on page 64:
Single channel 12.5 µl, 125 µl, 300 µl:
Use Fluorocarbon Gel (#100-00136-50) to lightly lubricate the
piston (a) without components slided onto. Put a drop of grease
onto your fingers, lubricate the black O-ring (b) at slide it over the
white seal (c).
Single channel 1250 µl and 5000 µl:
Use the Super-O-Lupe (#100-00135-50) to lightly lubricate the
outer ring of the Cup Seals (d, e). For the 5000 µl pipette also
lubricate the bottom of the white Cup Seal.
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VIAFLO Pipettes – Operating instructions V05 Maintenance
6.4 Calibration
The VIAFLO Pipettes are factory tested and calibrated under environmentally controlled
conditions using a gravimetric procedure in accordance with ISO 8655 standards. For the
accuracy and precision specifications, see “7.4 Pipette Specifications” on page 60.
Several conditions that would require recalibration of your pipette include a desire to:
• Optimize your pipette’s accuracy at a specific volume using aqueous liquid.
• Enable accurate displacement of non-aqueous based liquids (with different specific
gravities than water).
• Enhance accuracy when pipetting heated or cooled liquids.
6.4.1 Materials
• Precision balance with 0.01 mg readability (Ohaus AP250D)
• ASTM Class 2 or OIML E2 test weights
• Evaporation trap for balance
• Weighing vessel (optimally the height-to-diameter ratio is at least 3:1)
• Measurement equipment for temperature, humidity and atmospheric pressure (Testo
435-2 Professional)
• Distilled water (Grade 3 according to ISO 3696)
• New non-filtered GripTips matching the pipette volume (see section 8.2
• Single channel pipettor charging stand
• 2 beakers with distilled water
- 1 for pre-wetting
- 1 for calibration procedure
)
6.4.2 Definitions
Blow-out: Discharges any residual liquid from the tip.
Blow-in: After the blow-out, the piston moves back into home position. This causes a
slight intake of air (or liquid if tips remain in the liquid).
Two step blow-out: To avoid an early blow-in, keep the run button pressed while
dispensing, remove the tips from liquid and then release the run button.
Pre-wet: The action of pre-coating the inside of the liquid contacting parts with a thin film
of the same liquid. Additionally, it equilibrates humidity of the air space inside tip and the
pipette.
Touch off: Touching the pipette tip against the surface liquid or side of well-plate to
release any sample liquids that might be on the end of the pipettor tip.
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VIAFLO Pipettes – Operating instructions V05 Maintenance
6.4.3 Test Conditions and Environment
Tests and calibrations should be performed in conditions and environment according to
ISO 8655-6 standard.
• Temperature needs to be between 18–25 °C and remain constant (±0.5 °C) throughout the calibration.
• Optimal relative humidity of the environment is >50% and around the dispensing position 80%.
• GripTips, pipettes and distilled water need to be in the calibration laboratory for at
least 2 hours prior to calibration to reach temperature equilibrium with the environment.
• The balance must be validated using reference weights before and after a measuring
series. A low and high volume weight should be used. E.g.:
100 g (Mettler Toledo, #11119250)
10 g (Mettler Toledo, #11119220)
1 g ( M e t t l e r Toledo, #11119190)
10 m g ( M e t t l e r Toledo, #11119130)
AUTION
C
Always use new, pre-wetted GripTips for leak test and calibration.
6.4.4 Pre-wetting of tips
Pre-wet new GripTips three times prior to starting tests and calibrations. This is required
every time a tip is changed.
1) Attach a new unused GripTip which correspond to the volume range of the pipette
directly from the rack without touching the tip by hand.
2) Use a separate waste container for pre-wet dispenses.
3) In the Pipet mode, program the pipette to aspirate full volume. Set aspirating speed at
6. Aspirate by pressing the Run key.
4) Dispense: Keep the Run key pressed until all liquid is expelled, remove the tip from
the liquid and then release the Run key to perform a two-step blowout, see “4.3.2
Blowout modes” on page 21.
52 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Maintenance
6.4.5 Leak test
It is recommended to perform a leak test every 3 months or when errors occur.
1) Prewet tips as described above.
2) In the Pipet/Mix mode, program the VIAFLO Electronic Pipettes to aspirate full volume
at speed 6 and mix at full volume. Set to mix to 6 cycles and set mixing speed at 6.
3) Aspirate full volume and verify liquid level is not decreasing during 2-3 seconds while
GripTips are still in distilled water.
4) Remove the GripTips from the liquid and hold the pipette at a 30° angle. Wait for 20
seconds.
a) Observe whether liquid droplets are forming at the end of the GripTips.
b) On a multichannel pipette, verify liquid level is even across all channels.
5) Immerse GripTips approx. 2 mm into distilled water and press Run key to start mix
cycle.
a) Observe whether air bubbles are forming when dispensing.
b) On a multichannel pipette, check whether the levels stay approximately on the
same level across all channels.
c) At the end of a last dispense it is normal to have air bubbles because a blow-out is
performed.
For a VOYAGER pipette, carry out steps 1-5 for closed and open tip positions (min. and
max. tip spacing).
Signs indicating a leak
1) During the mix cycle in the leak test, the liquid level of 1 channel is decreasing.
OTE
N
A decreasing liquid level at aspiration could be an indication of a slow leak.
Performing a retest at 10 mixes may help identify a slow leak.
2) Liquid is left in a tip after the last dispense during the leak test.
3) One or more channels show air bubbles during the mix cycle in the leak test.
4) Droplets are forming when holding the pipette in the air for 20 seconds, even if a pre-
wet was performed.
5) The liquid levels are not equal on all channels after aspiration.
If the pipette is leaking, change O-rings (see 6.3.2
) and lubricate pistons (see 6.3.3) or
contact your service technician.
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VIAFLO Pipettes – Operating instructions V05 Maintenance
6.4.6 Obtaining the Actual Volume
INTEGRA certifies the pipettes at 10 %, 50 % and 100% of the nominal value. For each
volume 5 measurements are taken from all channels.
General
1) Always use new, unused GripTips, also when changing the test volume (e.g. from 125
µl to 12.5 µl).
2) Always pre-wet (section 6.4.4
measurement, first select the maximum volume for the pre-wet and then change to the
volume to be measured.
3) After dispensing, perform a touch off to make sure no liquid remains on the tip.
Gravimetric testing
1) Write down the ambient temperature and air pressure.
2) Perform a pre-wet, see 6.4.4
3) In the Pipet mode program the high test volume and set pipettor to dispense at speed
8 for 125 µl and 300 µl and speed 6 for 12.5 µl, 1250 µl and 5000 µl.
4) The first and second dispense to the balance should not be recorded. After each
dispense re-tare the balance.
5) Aspirate the Target volume of water keeping the pipette in a vertical to 30-degree
position while immersing the pipet tip 2–3 mm below the surface of the water. When
withdrawing the tip from the liquid, gently wipe the tip against the side wall of the vessel
to remove any liquid from the outside of the pipet tip.
6) Start the first measurement. Always pipet directly into the liquid of the weigh container
on the balance. During dispense, keep the run button pressed until the pipette is
removed again from the weigh vessel to perform a 2-step blowout. Record the weight
from the balance.
7) After completing the 5 high volume measurements, continue with the mid and low test
measurements by repeating steps 2-6.
) when using a new GripTip. For a low volume
.
54 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Maintenance
Ta rg e t V
s
Accuracy
Precision
V
Actual
VimiZ=
V
1
n
-- -
V
i
i 1=
n
=
6.4.7 Calculation of accuracy and precision
Accuracy
The ability of a pipettor to aspirate or dispense the exact volume desired. It indicates the
proximity of measurement results to the true value. Accuracy is also know as systematic
error and as the name indicates, can be corrected/calibrated.
Precision, Coefficient of variation (CV)
Precision indicates the repeatability or reproducibility of the measurement. It is also called
random error and is therefore an unpredictable error, which can not be corrected/
calibrated.
The following symbols are used throughout this text:
V
= Selected test or target Volume
s
m
= Measured Liquid Weight (g)
i
Z = Z factor, see 7.5
V
= Converted Volume (ml or µl)
i
V = Actual mean Volume (ml or µl)
n = Number of measurements
Conversion of the mass to volume
The values obtained by balance readings are in grams. These values need to be
converted to micro liters using the Z correction factor. It takes into account the water
density and air buoyancy during weighing at the corresponding test temperature. To
determine the correct Z factor, find the intersection between temperature and air pressure
in Table 7.5
Multiply each weight
. Round up temperature and air pressure values.
m
obtained in 6.4.6 with the corresponding Z factor:
i
Add together the volumes
mean volume
Actual Volume:
www.integra-biosciences.com 55
V (in milliliters or micro liters) delivered at the test temperature, which is the
V
delivered, divide the sum by n (e.g. n = 5) to calculate the
i
VIAFLO Pipettes – Operating instructions V05 Maintenance
e
s
VV
s
=
e
s
100 VV
s
V
s
-----------------------------------
=
s
r
ViV–
2
i 1=
n
n 1–
-------------------------------=
CV 100
s
r
V
----
=
Calculation of systematic error (Accuracy)
e
The systematic error
selected test volume:
or in percent:
Calculation of random error (Precision %)
To calculate the random error as the repeatability standard deviation s
equation:
The random error can also be expressed as a percentage, by the coefficient of variation
can be calculated using the following equation with V
s
, use the following
r
being the
s
CV, using equation:
If the pipette is properly calibrated, the Target volume
V within the accuracy specifications of the pipette.
6.4.8 Adjusting VIAFLO Electronic Pipettes
Compare the calculated accuracy and precision values with the corresponding pipette
specifications given in section 7.4
In case a VIAFLO Electronic pipette has not met the calibration specifications it needs to
be adjusted. This can easily be corrected by the pipettor’s software.
There are two modes for calibration of VIAFLO Pipettes, “Pipette Calibration Mode”, for
neat transfers and “Repeat Calibration Mode“ for pipetting aliquots.
The industry standard is to test and present specifications using neat transfers. This is
aspirating and dispensing the same volume. The “pipette” factor of the pipette’s software
determines the performance of neat transfers and is therefore adjusted in case a pipette
performs out of specifications. Adjusting a pipette in “Repeat Calibration Mode” is normally
not required and is not described in this document.
56 INTEGRA Biosciences AG
V
should equal the Actual volume
s
.
VIAFLO Pipettes – Operating instructions V05 Maintenance
The following example is for a 125 µl pipette.
Select Toolbox on the Main Menu. Select the Calibration & Service and then the
Calibration option. Press OK.
Highlight the Pipet Current Factor.
Press to edit the volume.
V
Highlight Target Volume (
menu. Press OK.
Use the touch wheel to enter the Target Volume.
Always use the nominal volume (100%) to adjust a
VIAFLO pipette (125 µl in this example). Press OK to
save your selection.
Move the cursor to the Actual Volume (
The actual volume is the mean of the weights corrected by the Z factor, resulting in V, the
mean volume in micro liters (see section 6.4.7
your selection.
A message will indicate that the pipette recalibration is complete. To check and revalidate,
repeat Steps 1–7 in Section 6.4.6
In case a calibration reminder was set, it can be reset in the Calibration Reminder menu
for either time and/or cycles.
OTE
N
In case an error message appears when setting the new correction factor,
follow these steps:
a) Select the Repeat factor in the calibration menu and enter the target
and actual (measured) volume.
b) Press calibrate.
c) Then select Pipet factor and enter target and actual volume.
d) Press calibrate.
e) Both factors should be the same now.
f) Press Save.
V). Use the touch wheel to enter the Actual Volume.
, 127 µl in this example). Press OK to save
Press to Calibrate and apply the factor universally
to all future Pipet mode motor movements. This will
correct for any measured inaccuracies observed in
the validation process. The Old and New Factors are
displayed. You are returned to the Calibration menu.
Press to Save your settings.
.
) on the Pipet Calib
t
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VIAFLO Pipettes – Operating instructions V05 Maintenance
6.5 Equipment disposal
The VIAFLO Electronic Pipettes must not be disposed of with unsorted
municipal waste.
Dispose of the VIAFLO Electronic Pipettes in accordance with the laws and
regulations in your area governing disposal of devices.
58 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Technical Data
7 Technical Data
7.1 Environmental conditions
Operation
Temperature range 5–40°C
Humidity range Max. rel. humidity 80 % for temperatures up to
31 °C, decreasing linearly to 50 % rel. humidity at
40 °C.
Altitude range < 2000 m
7.2 Specification of the device
Power Requirements 100–240 VAC, 50/60 Hz
Universal Power supply Input: 100–240V, 50/60Hz
Output: 6V, 0–1.75A DC (set at 0.5 A)
Pipetting channels single, 4, 6, 8, 12 or 16
Pipetting speed 10 steps
Pipetting technology Air displacement
User interface Touch wheel, color display
7.3 Pipetting speed
Pipette Size
Pipetting speed (micro liters per second)
Speed 12.5 µl 125 µl 300 µl 1250 µl 5000 µl
1 0.44 4.48 11.26 45.16 174.40
2 0.88 8.86 22.29 89.36 345.07
3 2.12 21.38 53.79 215.69 832.93
4 2.56 25.83 65.00 260.63 1006.46
5 3.00 30.24 76.10 305.12 1178.29
6 3.97 40.00 100.65 403.55 1558.39
7 5.35 53.91 135.65 543.91 2100.43
8 6.83 68.89 173.33 695.00 2683.89
9 7.69 77.50 195.00 781.88 3019.38
10 8.79 88.57 222.86 893.57 3450.71
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VIAFLO Pipettes – Operating instructions V05 Technical Data
7.4 Pipette Specifications
VIAFLO Electronic Pipettes Manufacturer ISO8655 limits
Vol ume
Incre-
ments
(µl)
0.1 12.5 3.00 1.00 12.80 4.80
0.5 30 2.00 0.60 13.33 5.00
1 125 3.00 0.60 12.80 4.80
5 500 3.00 0.75 8.00 3.00
0.1 12.5 3.75 1.50 25.60 9.60
0.5 30 4.00 1.20 26.67 10.00
1 125 6.00 1.10 25.60 9.60
Test
Vol ume
Accuracy
(µl)
6.25 1.50 0.80 3.20 1.60
12.5 1.00 0.40 1.60 0.80
62.5 1.20 0.40 2.56 0.96
125 0.60 0.20 1.28 0.48
150 1.00 0.30 2.67 1.00
300 0.60 0.15 1.33 0.50
625 1.00 0.30 2.56 0.96
1250 0.60 0.17 1.28 0.48
2500 1.20 0.30 1.60 0.60
5000 0.60 0.15 0.80 0.30
6.25 4.00 1.60 6.40 3.20
12.5 2.00 0.80 3.20 1.60
62.5 2.50 0.70 5.12 1.92
125 1.60 0.35 2.56 0.96
150 2.00 0.60 5.33 2.00
300 1.60 0.35 2.67 1.00
625 2.40 0.50 5.12 1.92
1250 1.20 0.30 2.56 0.96
(±%)
Precision
(≤%)
Accuracy
(±%)
Precision
(≤%)
Chan-
nel
140110.5–12.5 0.01 1.25 5.00 4.00 16.00 8.00
14012
14013
14014
14015
840210.5–12.5 0.01 1.25 10.00 6.00 32.00 16.00
84022
84023
84024
Part
No.
Vol ume
Range
(µl)
5.0–
125.0
10.0–
300.0
50.0–
1250.0
100.0–
5000.0
5.0–
125.0
10.0–
300.0
50.0–
1250.0
60 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Technical Data
VIAFLO Electronic Pipettes Manufacturer ISO8655 limits
Vol ume
Incre-
ments
(µl)
0.1 12.5 3.75 1.50 25.60 9.60
0.5 30 4.00 1.20 26.67 10.00
1 125 6.00 1.10 25.60 9.60
0.01 1.25 10.00 6.00 32.00 16.00
0.1 12.5 3.75 1.50 25.60 9.60
Test
Vol ume
Accuracy
(µl)
6.25 4.00 1.60 6.40 3.20
12.5 2.00 0.80 3.20 1.60
62.5 2.50 0.70 5.12 1.92
125 1.60 0.35 2.56 0.96
150 2.00 0.60 5.33 2.00
300 1.60 0.35 2.67 1.00
625 2.40 0.50 5.12 1.92
1250 1.20 0.30 2.56 0.96
6.25 4.00 1.60 6.40 3.20
12.5 2.00 0.80 3.20 1.60
62.5 2.50 0.70 5.12 1.92
125 1.60 0.35 2.56 0.96
(±%)
Precision
(≤%)
Accuracy
(±%)
Precision
(≤%)
Chan-
nel
12 4031 0.5–12.5 0.01 1.25 10.00 6.00 32.00 16.00
12 4032
12 4033
12 4034
16 4041
16 4042
The multichannel and VOYAGER pipettes’ specifications represent the performance of all
channels of the pipettes. Precision = Coefficient of Variation.
Part
No.
Vol ume
Range
(µl)
5.0–
125.0
10.0–
300.0
50.0–
1250.0
0.5–12.5
5.0–
125.0
www.integra-biosciences.com 61
VIAFLO Pipettes – Operating instructions V05 Technical Data
VOYAGER Tip Spacing Pipettes Manufacturer ISO8655 limits
(µl)
10.0–
300.0
50.0–
10.0–
300.0
50.0–
0.5–
12.5
5.0–
125.0
10.0–
300.0
50.0–
0.5–
12.5
5.0–
125.0
Vol ume
Incre-
ments
(µl)
0.5 30 5.00 2.00 26.67 10.00
0.5 30 5.00 2.00 26.67 10.00
0.01 1.25 15.00 10.00 32.00 16.00
0.1 12.5 6.00 3.50 25.60
0.5 30 5.00 2.00 26.67
0.01 1.25 15.00 10.00 32.00
0.1 12.5 6.00 3.50 25.60
Test
Volume
(µl)
1 125 6.00 1.50 12.80 4.80
1250 1.50 0.50 1.28 0.48
1 125 6.00 1.50 12.80 4.80
1250 1.50 0.50 1.28 0.48
1 125 6.00 1.50 12.80
1250 1.50 0.50 1.28
Accu-
300 1.80 0.50 2.67 1.00
300 1.80 0.50 2.67 1.00
12.5 2.50 1.60 3.20
62.5 3.00 1.00 5.12
125 2.00 0.70 2.56
150 2.75 1.00 5.33
300 1.80 0.50 2.67
625 3.00 0.75 2.56
6.25 5.00 3.20 6.40
12.5 2.50 1.60 3.20
62.5 3.00 1.00 5.12
125 2.00 0.70 2.56
racy
(±%)
Precision
(≤%)
Accu-
racy
(±%)
Precision
(≤%)
1.60
9.60
1.92
0.96
10.00
2.00
1.00
4.80
0.96
0.48
16.00
3.20
1.60
9.60
1.92
0.96
Tip
Spacing
(mm)
9.0–
32.5150 2.75 1.00 5.33 2.00
9.0–
32.5625 3.00 0.75 2.56 0.96
9.0–
19.5150 2.75 1.00 5.33 2.00
9.0–
19.5625 3.00 0.75 2.56 0.96
4.5–
14.06.25 5.00 3.20 6.40 3.20
4.5–
14.0
9.0–
14.0
9.0–
14.0
4.5–
9.0
4.5–
9.0
Chan-
nel
4 4143
4 4144
6 4163
6 4164
8 4121
8 4122
8 4123
8 4124
12 4131
12 4132
The multichannel and VOYAGER pipettes’ specifications represent the performance of all
channels of the pipettes. Precision = Coefficient of Variation.
Part
No.
Volume
Range
1250.0
1250.0
1250.0
62 INTEGRA Biosciences AG
VIAFLO Pipettes – Operating instructions V05 Technical Data
7.5 Z Correction Factors
Tem p.
(°C)
18.0
18.5
19.0
19.5
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
Z values in microliters per milligram
80 85 90 95 100 101.3 105
1.0022
1.0023
1.0024
1.0025
1.0026
1.0027
1.0028
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0037
1.0023
1.0024
1.0025
1.0026
1.0027
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0035
1.0036
1.0037
Air Pressure (kPa)
1.0023
1.0024
1.0025
1.0026
1.0027
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0038
1.0024
1.0025
1.0026
1.0027
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
1.0038
1.0025
1.0025
1.0026
1.0027
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0037
1.0039
1.0025
1.0026
1.0027
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0038
1.0039
1.0025
1.0026
1.0027
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
1.0038
1.0039
www.integra-biosciences.com 63
VIAFLO Pipettes – Operating instructions V05 Accessories
8 Accessories
8.1 Accessories
Charging options and Bluetooth Part No.
Universal voltage power supply 4200
Lithium ion battery, 3.7 V 4205
Single-pipettor charging stand 4210
VIALINK update stand 4211
4-pipettor charging stand 4215
Vision Bluetooth module 4220
Bluetooth PC module with PC software 4225
Universal voltage power supply for 4-pipettor charging stand 436-00002-00
General Part No.
O-ring removal tool 130-00731-00
8.2 Consumables
O-rings for tip fittings Part No.
300 μl Replacement kit 24/pack 100-00027-50
1250 μl Replacement kit 24/pack 100-00028-50
5000 μl Replacement kit 24/pack 100-00029-50
Lubricant for VIAFLO pipettes and O-rings Part No.
Lube Parker Super-O-Lube, Silicone based, 50 g / 2 oz, for single
channel 1250 µl and 5000 µl pipettes
Grease Grease Nye Fluorocarbon Gel 807, 5 g / 0.2 oz, for single
channel 12.5 µl, 125 µl and 300 µl pipettes
Reagent Reservoirs Part No.
10 ml Disposable reagent reservoirs, sterile, individually sealed,
30 reservoirs per case
Disposable reagent reservoirs, sterile, four sleeves of
50 reservoirs per case
Reservoir Base, 10 pack 4306
64 INTEGRA Biosciences AG
100-00135-50
100-00136-50
4331
4332
VIAFLO Pipettes – Operating instructions V05 Accessories
Reagent Reservoirs Part No.
25 ml Disposable reagent reservoirs, sterile, individually sealed,
30 reservoirs per case
Disposable reagent reservoirs, sterile, four sleeves of
50 reservoirs per case
Reservoir Base, 10 pack 4304
100 ml Disposable reagent reservoirs, sterile, individually sealed,
30 reservoirs per case
Disposable reagent reservoirs, sterile, four sleeves of
50 reservoirs per case
Reservoir Base, 10 pack 4305
GripTips for VIAFLO Electronic Pipettes Part No.
12.5 μl
LONG
12.5 μl 1 bag of 1000 tips, non-sterile, bulk pack 4411
125 μl 1 bag of 1000 tips, non-sterile, bulk pack 4421
1 bag of 1000 tips, non-sterile, bulk pack, LONG 4401
5 inserts of 384 tips, non-sterile, GREEN CHOICE, LONG 4402
5 boxes of 384 tips, non-sterile, LONG 4403
5 boxes of 384 tips, sterile, LONG 4404
5 boxes of 384 tips, sterile, filter, LONG 4405
5 inserts of 384 tips, pre-sterilized, GREEN CHOICE, LONG 4406
5 inserts of 384 tips, non-sterile, GREEN CHOICE 4412
5 boxes of 384 tips, non-sterile 4413
5 boxes of 384 tips, sterile 4414
5 boxes of 384 tips, sterile, filter 4415
5 inserts of 384 tips, pre-sterilized, GREEN CHOICE 4416
5 inserts of 384 tips, non-sterile, GREEN CHOICE 4422
5 boxes of 384 tips, non-sterile 4423
5 boxes of 384 tips, sterile 4424
5 boxes of 384 tips, sterile, filter 4425
5 inserts of 384 tips, pre-sterilized, GREEN CHOICE 4426
4311
4312
4321
4322
www.integra-biosciences.com 65
VIAFLO Pipettes – Operating instructions V05 Accessories
300 μl 1 bag of 1000 tips, non-sterile, bulk pack 4431
5 inserts of 96 tips, non-sterile, GREEN CHOICE 4432
5 boxes of 96 tips, non-sterile 4433
5 boxes of 96 tips, sterile 4434
5 boxes of 96 tips, sterile, filter 4435
5 inserts of 96 tips, pre-sterilized, GREEN CHOICE 4436
1250 μl 1 bag of 500 tips, non-sterile, bulk pack 4441
5 inserts of 96 tips, non-sterile, GREEN CHOICE 4442
5 boxes of 96 Tips, non-sterile 4443
5 boxes of 96 Tips, sterile 4444
5 boxes of 96 Tips, sterile, filter 4445
5 inserts of 96 tips, pre-sterilized, GREEN CHOICE 4446
5 ml 1 bag of 250 tips, non-sterile, bulk pack 4451
Individually wrapped, 100 tips per case, sterile 4456
66 INTEGRA Biosciences AG
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